Publications

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comparison of assays for mengovirus and reovirus-3. 196113701443
effect of actinomycin d on cellular nucleic acid synthesis and virus production.actinomycin d inhibits the synthesis of ribonucleic acid in l cells and the yield of vaccinia virus containing deoxyribonucleic acid, but it does not inhibit cellular deoxyribonucleic acid synthesis or the multiplication of mengo virus containing ribonucleic acid. these observations serve to distinguish the replication of viral ribonucleic from ribonucleic acid synthesis which is controlled by viral or cellular deoxyribonucleic acid.196113740412
the isolation of three variants of mengo virus differing in plaque morphology and hemagglutinating characteristics. 196113889897
localization of ribonucleic acid synthesis in mengovirus-infected l-cells. 196213894686
a cytochemical description of the multiplication of mengovirus in l-929 cells.a correlation of cytochemical changes with virus production has been studied in l cells infected with mengovirus. after a latent period of about 2 hours, virus was produced rapidly, reaching maximum titers of up to 12,000 particles per cell in 6 to 8 hours. the earliest cytological change was in the nucleus and consisted of a slight condensation of chromatin. there is no evidence, however, for the multiplication of either the viral rna or protein in the nucleus. rna, of high molecular weight, ac ...196213894687
some factors influencing thermal stability of mengo virus. 196213915619
ph and thermal stability of mengo virus. 196213915620
the effect of mengovirus infection on the activity of the dna-dependent rna polymerase of l-cells. 196213864524
a comparison of the changes in fine structure of l cells during single cycles of viral multiplication, following their infection with the viruses of mengo and encephalomyocarditis.the virus of encephalomyocarditis (emc), examined by the negative-contrast method, is indistinguishable from the serologically related mengovirus. the particles are 270 to 280 a in diameter. the surface of emc is composed of an undetermined number of subunits. frequent sampling of infected cells was carried out throughout one-step cycles of viral multiplication to observe cytopathic changes occurring in l cells infected by these two related rna viruses. emc and mengovirus, which multiply at equa ...196213883195
synthesis of rna in l cells infected with mengo virus. 196314064126
a new ribonucleic acid polymerase appearing after mengovirus infection of l-cells. 196314085393
mengovirus-induced inhibition of host ribonucleic acid and protein synthesis. 196314097404
effects of puromycin and p-fluorophenylalanine on mengovirus ribonucleic acid and protein synthesis. 196314097405
mengovirus replication. i. conservation of virus rna. 196414157500
mengovirus replication. ii. isolation of polyribosomes containing the infecting viral genome. 196414157502
studies on the mechanism of interferon action. ii. the effect of interferon on some early events in mengo virus infection in l cells. 196414166118
replication of mengovirus in chinese hamster ovary cells. la-3132-ms. 196414271615
the effect of mengovirus infection on the activity of the dna-dependent rna polymerase of l-cells. ii. preliminary data on the inhibitory factor. 19644284277
the effect of polyanions and polycations on mengo virus--l cell interaction. 19654285585
[viral growth and interferon production in mice on a starvation diet infected by mengo virus]. 19654286985
mengovirus replication. 3. virus reproduction in chinese hamster ovary cells. 19654283990
mengovirus replication. iv. inhibition of chinese hamster ovary cell division as a result of infection with mengovirus. 19654283992
intracellular fate of mengo virus ribonucleic acid.homma, m. (the wistar institute, philadelphia, pa.), and a. f. graham. intracellular fate of mengo virus ribonucleic acid. j. bacteriol. 89:64-73. 1965.-p(32)-labeled, purified preparations of mengo virus adsorbed rapidly and irreversibly to l cells maintained in suspension cultures. at intervals after adsorption of labeled virus, the total ribonucleic acid (rna) of infected cells was extracted by a phenol technique. infectivity titrations on this rna showed that it retained its full biological ...196514255683
symposium on replication of viral nucleic acids. 3. replication of mengovirus ribonucleic acid. 19664287031
replication of mengovirus. i. effect on synthesis of macromolecules by host cell.plagemann, peter g. w. (western reserve university, cleveland, ohio), and h. earle swim. replication of mengovirus. i. effect on synthesis of macromolecules by host cell. j. bacteriol. 91:2317-2326. 1966.-the replication of mengovirus was studied in two strains of novikoff (rat) hepatoma cells propagated in vitro. the replicative cycle in both strains required 6.5 to 7 hr. infection resulted in a marked depression of ribonucleic acid (rna) and protein synthesis by strain n1s1-63. inhibition of r ...19664287585
replication of mengovirus. ii. general properties of the viral-induced ribonucleic acid polymerase.plagemann, peter g. w. (western reserve university, cleveland, ohio), and h. earle swim. replication of mengovirus. ii. general properties of the viral-induced ribonucleic acid polymerase. j. bacteriol. 91:2327-2332. 1966.-mengovirus induces the appearance of a ribonucleic acid (rna) polymerase activity in novikoff hepatoma cells which is readily distinguished from the deoxyribonucleic acid (dna)-dependent rna polymerase since it is not inhibited by actinomycin d or deoxyribonuclease, but is inh ...19664287586
respiration of mengovirus-infected l-929 cells.iglewski, w. j. (the pennsylvania state university, university park), and e. h. ludwig. respiration of mengovirus-infected l-929 cells. j. bacteriol. 92:733-738. 1966.-polarographic techniques were employed to study the oxidative metabolism of l-929 cells during a one-step mengovirus growth cycle. virus maturation began 3.5 hr after infection and was complete with 7 hr. virus maturation was accompanied by a decreased rate of endogenous respiration and an increased rate of oxidation of succinate ...19664288496
effect of interferon on polymerization of single-stranded and double-stranded mengovirus ribonucleic acid.gordon, irving (university of southern california, los angeles), sara s. chenault, douglas stevenson, and jean d. acton. effect of interferon on polymerization of single-stranded and double-stranded mengovirus ribonucleic acid. j. bacteriol. 91:1230-1238. 1966.-the effect of interferon on actinomycin-resistant mengovirus ribonucleic acid (rna) replication in l cells was investigated to determine whether defective or partially polymerized rna products were made and whether synthesis of any specif ...19664286447
effect of cortisone on activation of lysosomal enzymes resulting from mengovirus infection of l-929 cells. 19664285387
inhibition of mengo virus by interferon.gauntt, charles j. (the university of texas, austin), and royce z. lockart, jr. inhibition of mengo virus by interferon. j. bacteriol. 91:176-182. 1966.-the inhibition of mengo virus replication in l cells resulting from interferon was studied quantitatively. interferon was titrated on l cells with western equine encephalomyelitis (wee) virus as the challenge virus. one protective unit (pu) of interferon is the least amount of interferon which prevents cytopathic effects when a large multiplicit ...19664285530
ribosomes: effect of interferon on their interaction with rapidly labeled cellular and viral rna's.rapidly labeled rna of mouse l cells and labeled rna of mengo virus, unlike cellular rna labeled under steady-state conditions, form detectable complexes with l-cell ribosomes. these ribosome-rna complexes formed in vitro appear analogous to those assembled during polysome formation in vivo. when ribosomes are prepared from l cells exposed to homologous interferon, their capacity to associate with cell messenger is preserved, while their ability to interact with viral rna is markedly reduced. th ...19676018644
cytopathology of mengovirus infection. i. relationship between cellular disintegration and virulence. 19674290639
cytopathology of mengovirus infection. ii. proliferation of membranous cisternae. 19674290640
a sensitive assay for infectivity of mengovirus rna in l cells. 19674291297
passage of mengovirus through the zona pellucida of the mouse morula. 19674291335
statolon-induced resistance of mice to mengovirus.mice receiving statolon intraperitoneally were 1,000 times more resistant to intraperitoneal challenge with mengovirus than were untreated controls. protection was afforded when statolon was administered 1 day before or 1 day after intraperitoneal inoculation with the virus. no therapeutic effect was observed when treatment with statolon was delayed for 2 days or more after virus infection. exposure of mice to a simulated space cabin environment did not increase their susceptibility to the letha ...19674291957
isolation of a series of mengo virus variants differing in sensitivity to polyanions. 19674291974
physico-chemical studies of three variants of mengo virus and their constituent ribonucleates. 19674292199
sensitivity to, and production of interferon by three variants of mengo virus. 19674292852
influence of heavy water (d2o) on the multiplication of adeno and mengo virus. 19674294141
treatment of mengovirus infection in mice with statolon.a single intraperitoneal injection of statolon was shown to exert a therapeutic effect on mice previously injected with the large plaque-forming variant of mengovirus. the ld(50) and survival time data demonstrated that such treatment was effective when given 2 to 48 hr after infection. no protective effect was apparent when statolon was administered 3, 4, or 5 days after the viral challenge. it was concluded that statolon, or other similar interferon inducers, may be of therapeutic value in ins ...19674294823
inhibition of rna synthesis in hela and l cells by mengovirus. 19674163399
[characterization of various representatives of the columbia-sk-virus group with fluorescent antibodies. i. the reproduction cycle of mengo virus in l cells]. 19674299732
protein leakage from mengovirus-infected cells. 196715938266
enhancement of mengovirus nucleic acid infectivity by putrescine. 19684300093
destruction of l cells by mengo virus: use of interferon to study the mechanism.interferon, when added to l cells, inhibited the synthesis of infectious mengo viral ribonucleic acid, hemagglutinins, and infectious virus by 85 to 95%. serum-blocking antigens were also reduced by the action of interferon, but threefold excess amounts of these antigens accumulated in interferon-treated cultures above the amounts expected for the quantity of infectious virus that was produced in these cultures. radioautographic analysis showed that 28 to 36% of the cells of an interferon-treate ...19684301505
replication of mengovirus in hela cells preinfected with nonreplicating poliovirus.the replication of mengovirus in hela cells preinfected with poliovirus in the presence of 10(-3) m guanidine was investigated. although host cell protein synthesis is inhibited by the presence of nonreplicating poliovirus, it is found that mengovirus ribonucleic acid (rna) and protein synthesis proceed normally under the same conditions. furthermore, no effects on mengovirus growth by poliovirus can be detected either when mengo protein synthesis is interrupted by acti-dione or when its rna syn ...19684302186
mengovirus replication in novikoff rat hepatoma and mouse l cells: effects on synthesis of host-cell macromolecules and virus-specific synthesis of ribonucleic acid.novikoff cells (strain n1s1-67) and l-67 cells, a nutritional mutant of the common strain of mouse l cells which grows in the same medium as n1s1-67 cells, were infected with mengovirus under identical experimental conditions. the synthesis of host-cell ribonucleic acid (rna) by either type of cell was not affected quantitatively or qualitatively until about 2 hr after infection, when viral rna synthesis rapidly displaced the synthesis of cellular rna. the rate of synthesis of protein by both ty ...19684176992
reversible inhibition of induction of mengovirus rna polymerase and of virus maturation in novikoff rat hepatoma cells by phenethyl alcohol. 19684295916
duration of prophylactic effect of statolon against mengovirus in mice.intraperitoneal injection of statolon into mice is known to exert a protective effect against a subsequent infection with a variety of viruses owing to stimulation of production of interferon. to study the duration of such protection, groups of mice were treated with 350 mug of statolon at 69, 55, 41, 27, 13, 6, or 1 day prior to a challenge with mengovirus. other groups were treated with 760 mug at 1 to 6 days before challenge. ten days after inoculation with the virus, ld(50) determinations we ...19684297000
effect of statolon-induced interferon on antibody formation.the effect of statolon-induced interferon on immune response was investigated. young adult swiss albino mice were immunized with chicken red blood cells or infectious mengovirus. the red blood cells were injected three times and the virus twice at 2-week intervals, with or without treatment with statolon 24 hr before the inoculations. the effect of interferon on antibody formation was measured by determinations of hemagglutinating, hemolytic, and virus-neutralizing antibodies in the sera of trea ...19684298812
delayed death associated with statolon treatment of mice infected with mengovirus. 19685718719
synthesis of ribonucleic acid by mengovirus-induced rna polymerase in vitro: nature of products and of rnase-resistant intermediate. 19684329736
physical and chemical studies of mengo virus variants. ii. chromatographic behavior and chemical composition. 19694304576
the interaction of tritium-labelled mengo virus rna and l cells: the effects of dmso and deae-dextran. 19694305634
utilization of substrate analogs by mengovirus induced rna polymerase. 19694305637
[induction of interferon synthesis by double-stranded rna. i. application to the study of the multiplication cycle of mengo virus]. 19694307590
influence of statolon on resistance of mice to influenza.various interferon inducers are known to elicit protection against lethal or infecting doses of certain viral agents. because of the relatively high morbidity rate of influenza and its seasonal occurrence, we wished to determine whether statolon-induced interferon might be effective in controlling this disease. mice were treated intraperitoneally with statolon and challenged with influenza a(2) virus by the intranasal route. although interferon was present in the serum at the time of virus admin ...19694308934
mengovirus-induced cytopathic effect in l-cells: protective effect of interferon.the effect of interferon on mengovirus-induced cytopathic effect (cpe) in l cells, the cut-off of host-cell protein synthesis, and production of mature virus were found to be dependent on the concentration of interferon. cpe and inhibition of host protein synthesis were not affected until the concentration of interferon was increased 100-fold over that required to reduce viral yields by 90%.19694309883
competitive inhibition of uridine incorporation by 6-azauridine in uninfected and mengovirus-infected novikoff hepatoma cells. 19694311690
host-dependent restriction of mengovirus replication.mengovirus infection of a restrictive cell line, maden's bovine kidney (mdbk), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as l cells or ehrlich ascites tumor cells (eat). cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral ...19694311796
insensitivity of mitochondrial rna synthesis to mengovirus infection in cho cells. 19694311805
[fluorescence serology studies on the effects of antiviral agents on virus replication. i. the action of beta-2-dl-thienyl alanine on the replication of mengo virus]. 19694313030
effect of prehumidification on sampling of selected airborne viruses.studies were undertaken to determine if a prewetting device (humidifier bulb) used in combination with an all glass impinger (agi-30) would increase the recovery of airborne mengovirus-37a, vesicular stomatitis virus (vsv), and the s-13 coliphage. suspensions of t3 coliphage with mengovirus-37a, vsv, or s-13 were aerosolized and collected by using the agi-30-humidifier bulb combination to sample the aerosols before and after shifts in relative humidities (rh). these studies revealed the followin ...19694313171
the structural proteins of mengo virus variants. 19704314506
polyacetal carboxylic acids: a new group of antiviral polyanions.chlorite-oxidized oxypolysaccharides are polyacetal carboxylic acids. they inhibited the cytopathic effect of vesicular stomatitis virus in mouse embryo cell cultures challenged at low input multiplicity. after intraperitoneal injection of these compounds in mice, interferon appeared in the circulation. the compounds also protected mice against lethal mengovirus infection and against the development of experimental pox lesions on the tail. chlorite-oxidized oxyamylose was antiviral only when at ...19704314553
antiviral activity of chlorite-oxidized oxyamylose, a polyacetal carboxylic acid.intraperitoneal injection of chlorite-oxidized oxyamylose (coam) protected mice against mengo, vaccinia, semliki forest, and influenza apr8 viruses. topical administration in the eye of rabbits partially inhibited the development of experimental herpetic keratoconjunctivitis. coam resembled polyacrylic acid in many aspects, but it was markedly less toxic. for systemic administration, the therapeutic index was on the order of magnitude of 1:300 to 1:500. although the in vivo antiviral effect of c ...19704314554
physical and chemical studies of mengo virus variants. 3. absorbance--temperature profiles, sedimentation in dextran sulfate gradients, and total-infectious particle ratios. 19704315866
inhibition of synchronized cellular deoxyribonucleic acid synthesis during newcastle disease virus, mengovirus, or reovirus infection.cultures of l cells were synchronized with respect to deoxyribonucleic acid (dna) synthesis with thymidine and 5-fluoro-2'-deoxyuridine (fudr) and infected with newcastle disease virus (ndv), mengovirus, or reovirus 3. inhibition of incorporation of (3)h-cytidine into the dna of synchronized cells is partially inhibited 2 hr after infection with ndv or mengovirus and nearly completely suppressed 4 hr after infection. with ndv and mengovirus, no evidence was obtained of differences in sensitivity ...19704317111
effect of statolon on aerogenic immunization of mice with mengovirus-37a. 19704317263
circular dichroism studies on mengo virus variants and their constituent ribonucleates. 19704318119
studies of the protein subunit of ph-inactivated mengo virus variants. i. polypeptide composition. 19704318981
mengovirus rna synthesis in productive and restrictive cell lines. 19704318986
influence of blood clearance rates on interferon production and virulence of mengo virus plaque mutants in mice. 19704319089
comparison of agar and agarose preparations for mengovirus plaque formation.an agarose overlay yielded mengovirus plaques earlier and in greater size and number than overlays of chemically undefined agars with or without enhancers. marked variability in plaque-forming efficacy of commercial agarose preparations was noted.19704320349
growth of mouse l cells in shaken culture and mengovirus plaque formation on l cells suspended in agar.procedures are described that require a minimum of equipment and maintenance for growing mouse l cells suspended in liquid medium and for plaquing mengovirus on l cells suspended in agar. viability of l cells during storage for 1 to 2 hr at relatively high concentrations was better in media at 30 c than at 0 c, as measured by viable counts after growth for 24 hr at 35 c. the number and size of plaques increased with increasing concentration of nahco(3) in the agar layers, but the relative differ ...19704320864
abnormal dna patterns in animal mitochondria: ethidium bromide-induced breakdown of closed circular dna and conditions leading to oligomer accumulation.treatment of cultured mouse fibroblasts (l-cells) and hamster kidney cells with ethidium bromide was found to inhibit the incorporation of [(3)h]thymidine into mitochondrial closed-circular dna, but not into nuclear dna. the specific activity of nuclear dna in treated cells was higher than in control cells. treatment also led to breakdown of closed-circular mitochondrial dna, greatly enlarged mitochondrial profiles with few or no cristae, and a higher yield of mitochondrial protein per cell.a si ...19704321352
effect of mengovirus replication on choline metabolism and membrane formation in novikoff hepatoma cells.infection of novikoff rat hepatoma cells (subline nlsl-67) with mengovirus resulted in a two- to threefold increase in the rate of choline incorporation into membrane phosphatidylcholine at about 3 hr after infection, without affecting the rate of transport of choline into the cell or its phosphorylation. the time course of virus-stimulated phosphatidylcholine synthesis was compared with the time courses of other virus-induced processes during a single cycle of replication. the formation of vira ...19704322083
[electron microscopic detection of mengovirus in l cells with ferritin-labeled antibodies]. 19704322839
the step in cellular dna synthesis blocked by newcastle disease or mengovirus infection. 19704312372
[interferon-inducing capacity of double-stranded rna. ii. studies on mengo virus replicatative intermediate]. 19704312964
delay of mengovirus-induced cytopathology in mitotic l-cells.the time course and extent of mengovirus production were the same in metaphase-arrested and interphase l-cells, yet the expression of cytopathology was delayed several hours in metaphase cells.19704194170
the interaction of mengo virus ribonucleic acid with l, hela and human diploid cells: a comparative study. 19705438061
transport as the rate-limiting step in the incorporation of uridine into mengovirus ribonucleic acid in novikoff rat hepatoma cells.the incorporation of uridine into the nucleotide pool of actinomycin-treated, mengovirus-infected novikoff rat hepatoma cells in culture follows simple michaelis-menten kinetics, and the apparent v(max) and k(m) values are similar to those for uridine transport by uninfected cells. incorporation of uridine into mengovirus-specific ribonucleic acid (rna) also follows michaelis-menten kinetics, and the apparent k(m) (about 10 mum) is approximately the same as for uridine transport. inhibition of u ...19715543426
effects of elevated temperatures on mengovirus ribonucleic acid synthesis and virus production in novikoff rat hepatoma cells.the production of mengovirus in novikoff rat hepatoma cells is progressively reduced with an increase in incubation temperature of the cells from 34 to 40 c, in spite of the fact that about the same amounts of single-stranded and double-stranded viral ribonucleic acid (rna) are synthesized at 34, 37, and 40 c; the rate of overall protein synthesis is as high at 40 c as at 37 c. at 40 c, progeny viral rna accumulates in an undegraded form without being incorporated into virus particles. the resul ...19715543427
[antiviral activity of imidazole derivates. 3. autoradiographic studies on the influence of 1-butyl-4(4'-allyloxyphenyl)-imidazole-hydrochloride on rna synthesis in mengovirus infected fl cells]. 19714330137
system of double infection between vaccinia virus and mengovirus.when l cells are simultaneously infected with vaccinia virus and mengovirus, double interference in the replication of both viruses is observed. superinfection of vaccinia virus-infected cells by mengovirus during the first 5 hr of infection reduces vaccinia virus yields to between 1 and 3% of controls. the yields of mengovirus are reduced to between 1 and 16% of controls, depending upon the time of superinfection. the replication of vaccinia deoxyribonucleic acid is not inhibited by mengovirus; ...19714330362
studies of the protein subunit of ph-inactivated mengo virus variants. ii. physicochemical properties. 19714330368
cellular changes attending mengovirus-induced cytolysis of mouse l-cells. 19714108194
immunofluorescent studies on the effect of antiviral substances on virus multiplication. ii. determination of fluorescence intensity in mengo virus infected l cells treated with beta-2-dl-thienylalanine. 19714325975
immunofluorescent studies on the effect of antiviral substances on virus multiplication. 3. effect of actidione on the multiplication of mengo virus. 19714325976
[demonstration of the mengo virus antigen in the mouse brain by help of the immunofluorescence technique]. 19714327126
[antiviral activity of imidazole derivatives. i. inhibition of multiplication of mengovirus in fl cells]. 19714329638
repair replication of hela cell deoxyribonucleic acid in cells infected with newcastle disease virus or mengovirus or treated with puromycin.we examined repair replication of hela cell deoxyribonucleic acid (dna) in cells infected with mengovirus or newcastle disease virus or treated with puromycin. cellular dna was damaged by ultraviolet light and then pulse-labeled with (3)h-thymidine. autoradiographic analysis of non-s-phase dna synthesis (repair replication) showed that there was no inhibition of this process at a time when overall cellular dna synthesis was severely inhibited by either virus infection or puromycin treatment.19714331658
a comparison of the protective effect against mengo virus infection in mice of interferons induced by polyi:c and mengo virus. 19714333910
in vitro translation of cardiovirus ribonucleic acid by mammalian cell-free extracts.cell-free extracts prepared from ehrlich ascites and mouse l cells synthesize viral proteins in response to encephalomyocarditis virus, mouse elberfeld virus, and mengovirus ribonucleic acid. although hela cell extracts are inactive, their ribosomes are functional in the presence of heterologous supernatant fractions. synthesis depends upon the addition of adenosine triphosphate, guanosine triphosphate, an energy-generating system, and 4 mm mg(2+). initiation is completed during the first 10 to ...19724336562
differential inhibition of nuclear rna polymerases in l cells infected with mengovirus. 19724338796
rna polymerase complexes from mengovirus infected cells. 19724341300
capsid polypeptides of mouse elberfeld virus. i. amino acid compositions and molar ratios in the virion.the four major polypeptide chains (alpha, beta, gamma, delta) constituting the capsid protein of mouse elberfeld (me) virus were isolated by preparative electrophoresis on polyacrylamide gels, and the amino acid composition of each chain was determined. in addition, the molecular weights of the smallest chains of me virus, mengovirus, and poliovirus, which had previously been determined by gel electrophoretic methods, were redetermined by gel filtration chromatography in 6 m guanidine hydrochlor ...19724342047
molcular species of interferon induced in mice by poly i:c and mengo virus. 19724343060
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