Publications

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exogenous porcine viruses.porcine organs, cells and tissues provide a viable source of transplants in humans, though there is some concern of public health risk from adaptation of swine infectious agents in humans. limited information is available on the public health risk of many exogenous swine viruses, and reliable and rapid diagnostic tests are available for only a few of these. the ability of several porcine viruses to cause transplacental fetal infection (parvoviruses, circoviruses, and arteriviruses), emergence or ...200312934944
[an intensification of antiviral and interferon effects of ridostin (an interferon inducer) by cationic liposomes in vitro and in intranasal administration].combined application of ridostine with catonic liposomes was shown to essentially enhance the interferon-inducing and antiviral activity of the former in experiments with cell cultures l-929, which is apparently related with an improved efficiency of intracellular delivery of dsrna. a comparative study demonstrated that ridostine, when combined with liposomes, is needed by 10(3)-10(4) times less as when it is used alone. a pretreatment of the cellular monolayer by cationic liposomes contributes ...200312945208
myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2.porcine circovirus 2 (pcv2) is a recently identified agent that has been associated with postweaning multisystemic wasting syndrome (pmws) in swine populations. in this report, the potential spectrum of disease associated with pcv2 is expanded by evidence of vertical transmission and associated reproductive failure. pcv2 was isolated from a litter of aborted piglets from a farm experiencing late-term abortions and stillbirths. severe, diffuse myocarditis was present in 1 piglet associated with e ...199912968736
the susceptibility of mice infected with cryptococcus neoformans to encephalomyocarditis virus. 195313069406
experimental studies on the pathogenesis of encephalomyocarditis virus for mice. i. the factor of sex of animal as affecting pathogenicity of inoculum and susceptibility to infection. 195313069474
encephalomyocarditis virus infection in childhood. 195513273129
transmission studies with encephalomyocarditis virus. 195613295631
recent isolations of encephalomyocarditis virus. 195613297734
[biochemical study of experimental encephalomyocarditis virus infection; behavior of various enzyme activities in the brain; liver and myocardium (adenylpyrophosphatase, succinodehydrogenase, aspartico-ketoglutaric transaminase, rhodanese)]. 195513327894
[behavior of some enzyme activities in the tissues (adenylpyrophosphatase, rhodanese) in various stages of experimental encephalomyocarditis virus infection]. 195513327895
effects of animal viruses on host cell metabolism. i. effects of encephalomyocarditis virus on nucleic acids and phosphorus fractions of ehrlich ascites tumor cells. 195613346122
propagation of mouse encephalomyocarditis virus in ascites tumour cells maintained in vitro. 195713436721
the isolation of encephalomyocarditis virus from aotus monkeys. 195713470204
[culture of the encephalomyocarditis virus mm in rotating tubes]. 195713506871
an infective ribonucleic acid component from tumour cells infected with encephalomyocarditis virus. 195813577895
[dependence of blood titers on the antigen concentration in vitro neutralization tests with encephalomyocarditis virus]. 195813604895
studies of mouse polyoma virus infection. iv. evidence for mucoprotein erythrocyte receptors in polyoma virus hemagglutination.treatment of guinea pig erythrocytes with types a and b influenza viruses rendered them inagglutinable by polyoma virus; also, the inhibitory effect of ovomucin on polyoma virus hemagglutination was destroyed by pretreatment of the ovomucin with various myxoviruses. these results indicate that polyoma virus and myxovirus erythrocyte receptor sites are identical. however, no destruction by polyoma virus of its own or of myxovirus receptors or inhibitors was detected. no serologic relationship was ...195913664870
studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 2. the isolation, crystallization and chemical characterization of mouse encephalomyocarditis virus. 196113698341
plaque-type mutants of murine encephalomyocarditis virus. 196113702427
a survey for antibodies to encephalomyocarditis virus in man and animals. 196013737081
studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 1. encephalomyocarditis virus in krebs ii mouse-ascites-tumour cells. 196113767335
serosurvey of encephalomyocarditis virus neutralizing antibodies in southern louisiana and peruvian indian populations. 196113790625
[demonstration of the existence of the encephalomyocarditis virus in the city of guayaquil]. 195913796498
characteristics of growth of encephalomyocarditis virus in cultures of mouse muscle. 195913809164
crystallization of purified mouse encephalomyocarditis virus particles. 196013821724
infective ribonucleic acid component of cells infected by encephalomyocarditis virus. 196213866887
a study of the mechanisms of dna and thymine degradation in cultured human cells infected with a lipovirus.dna and thymine degradation on cultured human, mouse, and chick cells were studied. significant increase in dna-degrading activity was demonstrated in human embryonic cells killed by freeze-thawing, liver cells killed with mitomycin c, mouse embryonic cells infected with encephalomyocarditis virus, and in all cells killed by the lipovirus. twelve other viral agents, actinomycin d, and 5-fluorodeoxyuridine failed to produce a similar increase. thymidine-2-c(14)-labeled cultures, either live, kill ...196213878103
encephalomyocarditis virus hemagglutination-inhibition test using antigens prepared in hela cell cultures. 196113881978
intracellular sites of synthesis of encephalomyocarditis virus components in krebs-2 ascites tumour cells. 196313970486
[the oncolytic effect on ehrlich's carcinoma of ribonucleic acid extracted from mouse encephalomyocarditis virus]. 196313985624
[action of ribonucleic acid extracted from mouse encephalomyocarditis virus (mm) on mouse ehrlich ascites carcinoma]. 196213985626
effect of "sex" of tissue cultures on growth of encephalomyocarditis virus. 196114005765
sedimentation coefficients of encephalomyocarditis virus and its ribonucleic acid. 196314017133
oral infection of swine with the encephalomyocarditis virus. 196314023638
the basis for the size differences in plaques produced by variants of encephalomyocarditis (emc) virus. 196314059823
facilitation of infection of mouse l-cells with ribonucleic acid from encephalomyocarditis virus. 196314070339
replicative form of encephalomyocarditis virus ribonucleic acid. 196314074552
ribonucleic acids in virus-infected and uninfected krebs ii ascites tumor cells. 196314086733
[encephalomyocarditis (emc) viruses. antibody determinations on healthy subjects in the halle area]. 196314091776
mengovirus-induced inhibition of host ribonucleic acid and protein synthesis. 196314097404
effects of puromycin and p-fluorophenylalanine on mengovirus ribonucleic acid and protein synthesis. 196314097405
reovirus type 3 infection in laboratory mice. 196314100789
demonstration of encephalomyocarditis virus antibody in human serums from panama. 196314104611
possible role of interferon in determining the oncogenic effect of polyoma virus variants.1. infection of newborn c(3)hf/bi mice with both the highly oncogenic s variant and the poorly oncogenic m variant of polyoma virus caused significantly fewer tumors than infection with the s variant alone 2. infection of newborn c(3)hf/bi mice at birth with either m or s variant caused resistance to infection with a heterologous agent, encephalomyocarditis virus, but the m variant caused a somewhat greater degree of resistance. 3. extracts of tissues of animals infected at birth with the m vari ...196414113116
effect of purine analogs on encephalomyocarditis and vaccinia virus growth in analog sensitive and resistant cells. 196414117421
mengovirus replication. i. conservation of virus rna. 196414157500
mengovirus replication. ii. isolation of polyribosomes containing the infecting viral genome. 196414157502
ascites tumours from bhk.21 cells transformed in vitro by polyoma virus. 196414161203
isolation of a virus resembling encephalomyocarditis from a red squirrel. 196414161241
clinical aspects of enterovirus infection. 196414163253
studies on nuclear ribosomes. ii. transfer of ribosomes from nucleus to cytoplasm in the early stages of viral infection. 196414165597
[the action of some physical and chemical agents on the infectivity of nucleic acids isolated from viruses. iv. chromatographic and spectrophotometric investigations of some ribonucleic acids extracted from the mm virus and exposed to the action of heat]. 196414184552
purification of murine encephalomyocarditis virus. 196414187906
propagation of encephalomyocarditis virus in swine embryo kidney cells. 196414194611
[causative agent of ornithosis and other representatives of chlamydiaceae]. 196414198179
the amino acid composition of three plaque-type mutants of encephalomyocarditis virus. 196414201149
biological and biochemical properties of the analogue antibiotic tubercidin. 196414206615
the fate of rapidly labelled ribonucleic acid in the presence of actinomycin in normal and virus-infected animal cells. 196414211634
development of ribonucleic acid polymerase in cells infected with encephalomyocarditis virus and the synthesis of single- and double-stranded rna by the isolated polymerase. 196414212417
protective effect of antibody or elevated temperature on intracerebral infection of mice with encephalomycarditis virus. 196414214388
studies of three variants of mengo encephalomyelitis virus. i. rate of attachment to l cells, and effect of ph on infectivity. 196414227050
inhibition of interferon production in hamster cells transformed in vitro with carcinogenic hydrocarbons. 196414227052
polyoma virus and l cell relationship. ii. a curable carrier system not dependent on interferon. 196414231150
interferon production in vitro by cells infected with the murine salivary gland virus. 196414233484
effect of 5-iodo-2'-deoxyuridine on the pathogenesis of columbia-sk virus in mice. 196414239948
antibodies to viruses of the columbia sk group in sciuridae in britain. 196414240114
intracellular fate of mengo virus ribonucleic acid.homma, m. (the wistar institute, philadelphia, pa.), and a. f. graham. intracellular fate of mengo virus ribonucleic acid. j. bacteriol. 89:64-73. 1965.-p(32)-labeled, purified preparations of mengo virus adsorbed rapidly and irreversibly to l cells maintained in suspension cultures. at intervals after adsorption of labeled virus, the total ribonucleic acid (rna) of infected cells was extracted by a phenol technique. infectivity titrations on this rna showed that it retained its full biological ...196514255683
electrophoretic separation of the proteins of ribosome subunits and of encephalomyocarditis virus. 196414257698
[new light on the mode of action of oral immunization]. 196514267524
replication of mengovirus in chinese hamster ovary cells. la-3132-ms. 196414271615
infection of escherichia coli k-12 with rna of encephalomyocarditis virus. 196514282021
[comparative biological and physico-chemical studies of some nucleic acids exposed to the action of heat]. 196414297947
the role of interferon in tick-borne encephalitis virus-infected l cells. i. acute infection. 196514299351
the induction of mutations in encephalomyocarditis virus with nitrous acid. 196514299357
the role of interferon in tick-borne encephalitis virus-infected l cells. ii. persistent infection. 196514299366
the intracellular site of formation of encephalomyocarditis virus ribonucleic acid. 196514304870
[isolation by column chromatography of infectious ribonucleic acid extracted from the brain of a mouse inoculated with murine encephalomyocarditis virus]. 196414309285
[column fractionation of ribonucleic acid extracted from murine encephalomyocarditis viruses and exposed to the action of heat]. 196414309286
studies on emc viral rna synthesis and its localization in infected krebs ascites cells. 196514319717
a density mutant of encephalomyocarditis virus. 196514322748
studies on the biological properties and classification of sv4 virus. 196514323044
some properties of the encephalomyocarditis, columbia sk and mengo viruses. 196514328905
studies of three variants of mengo encephalomyelitis virus. 3. effect of overlay and polyanions of plaque size. 196514329134
sites of synthesis of encephalomyocarditis virus components in infected l-cells. 196514331701
studies on protein and nucleic acid metabolism in virus-infected mammalian cells. the formation of a virus-specific antigen in krebs ii ascites-tumour cells infected with encephalomyocarditis virus.1. krebs ii mouse ascites-tumour cells infected with encephalomyocarditis virus were found to contain, in addition to mature virus, a virus-specific protein antigen. an assay, based on the ability of this antigen to block the neutralization of purified virus by its specific antiserum, was developed. 2. this antigen was present both in the culture fluid 17 hr. after the infection of cells with virus and intracellularly, where its titre increased at a time when viral capsid protein was being synth ...196514348194
fatal disease of swine due to encephalomyocarditis virus.encephalomyocarditis virus was isolated from the organs of swine dying during an outbreak of an acutely fatal disease occurring on a farm in panama. the outstanding lesion was severe myocarditis. pigs inoculated with the viral isolate developed a systemic infection with myocarditis.196014425363
role of infective nucleic acid in the production of encephalomyocarditis virus. 196014441395
action of adenine and related compounds on replication of encephalomyocarditis virus in tissue culture. 196214466920
studies on protein and nucleic acid metabolism in virus-infected mammalian cells. iv. the localization of metabolic changes within subcellular fractions of krebs ii mouse-ascites-tumour cells infected with encephalomyocarditis virus. 196114470360
studies on protein and nucleic acid metabolism in virus-infected mammalian cells. 5. the kinetics of synthesis of virus protein and of virus ribonucleic acid in krebs ii mouse-ascites-tumour cells infected with encephalomyocarditis virus. 196214470361
[titration of the infectious ribonucleic acid of the mouse encephalomyocarditis virus on ascites krebs ii cells in vitro]. 196214475450
antiviral effects of sulfated exopolysaccharide from the marine microalga gyrodinium impudicum strain kg03.the sulfated exopolysaccharide p-kg03, which is produced by the marine microalga gyrodinium impudicum strain kg03, exhibited impressive antiviral activity in vitro (ec50 = 26.9 micro g/ml) against the encephalomyocarditis virus (emcv). depending on the p-kg03 concentration, the development of cytopathic effects in emcv-infected hela cells was either inhibited completely or slowed. moreover, p-kg03 did not show any cytotoxic effects on hela cells, even at concentrations up to 1000 microg/ml. the ...200414508657
construction of a bicistronic proangiogenic expression vector and its application in experimental angiogenesis in vivo.manipulation of angiogenesis in vivo is an example of successful gene therapy strategies. overexpression of angiogenic genes like vegf, fgf or pdgf causes new vessel formation and improves the clinical state of patients. gene therapy is a very promising procedure but requires large amounts of pharmaceutical-grade plasmid dna. in this regard we have constructed a bicistronic plasmid dna vector encoding two proangiogenic factors, vegf165 and fgf-2. the construct (pvif) contains the internal riboso ...200314515168
susceptibility of primary culture neurons from rats of different ages to encephalomyocarditis (emc) virus infection.the changes in susceptibility of neurons to the d variant of emc virus (emc-d) (10(6) pfu/well) were investigated in developing hippocampal primary cultures from postnatal days of 1, 7, and 56 fischer 344 rats (p1, p7, and p56) for up to 12 h after infection (12 hai). the virus titer of primary culture neurons increased at 1 hai, decreased at 2 hai, increased at 3 hai, peaked at 8 hai, and decreased at 12 hai in all age groups. the titers at 1 and 8 hai were lowest in p56 cultures. the virus tit ...200314516779
analysis of t-cell receptor vbeta gene from infiltrating t cells in insulitis and myocarditis in encephalomyocarditis virus-infected balb/c mice.encephalomyocarditis (emc) virus induces insulin-dependent diabetes and myocarditis in several strains of mice. the t-cell receptor (tcr) vbeta genes of infiltrating t cells in the pancreas and myocardium of balb/c mice infected with emc virus d-variant (emc-d virus) were analyzed. using a nested two-step polymerase chain reaction (pcr), tcr vbeta cdnas were cloned and sequenced. two and four kinds of tcr vbeta clones were obtained from t cells infiltrating into the pancreas and myocardium of ba ...200314550273
assays for antiviral activity. 200414573896
gene expression of cardiac mast cell chymase and tryptase in a murine model of heart failure caused by viral myocarditis.this study examined the gene expression of mouse mast cell proteases to clarify their role in the pathophysiology of viral myocarditis. male dba/2 mice were inoculated intraperitoneally with the encephalomyocarditis virus and the gene expression of mast cell chymase, mouse mast cell protease (mmcp)-4 and -5, and tryptase, mmcp-6, matrix metalloproteinase (mmp)-9 and type-i procollagen was measured by real-time quantitative rt-pcr analysis. the gene expression of mmcp-4, -5 and -6 mrna was increa ...200314578624
conserved nucleotides within the j domain of the encephalomyocarditis virus internal ribosome entry site are required for activity and for interaction with eif4g.the internal ribosome entry site (ires) elements of cardioviruses (e.g., encephalomyocarditis virus [emcv] and foot-and-mouth disease virus) are predicted to have very similar secondary structures. among these complex rna structures there is only rather limited complete sequence conservation. within the j domain of the emcv ires there are four highly conserved nucleotides (a704, c705, g723, and a724)., which are predicted to be unpaired and have been targeted for mutagenesis. using an ires-depen ...200314610168
functionalized alkyl and aryl diselenides as antimicrobial and antiviral agents: synthesis and properties.the different dialkyl and diaryl diselenides with carbamoyl and sulfamoyl moieties 2, 3, 5 and other substituents in the ortho position of benzene ring 4, 7, 8 as well as derivatives of 1,2,4-benzoselenadiazine (6) were designed as antiviral and antimicrobial agents and synthesized. some of them, particularly 8a and 8b, were found in the antiviral assay in vitro to be strong inhibitors of cytopathic activity encephalomyocarditis virus (emcv). the compound 4a and 8a were found to have a broad spe ...200314630233
innate immune response to encephalomyocarditis virus infection mediated by cd1d.cd1d-reactive natural killer t (nkt) cells can rapidly produce t helper type 1 (th1) and/or th2 cytokines, can activate antigen-presenting cell (apc) interleukin-12 (il-12) production, and are implicated in the regulation of adaptive immune responses. the role of the cd1d system was assessed during infection with encephalomyocarditis virus (emcv-d), a picornavirus that causes acute diabetes, paralysis and myocarditis. emcv-d resistance depends on il-12-mediated interferon-gamma (ifn-gamma) produ ...200314632651
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