| thermal and water source effects upon the stability of enteroviruses in surface freshwaters. | the long-term survival of three human enterovirus serotypes, coxsackievirus b3, echovirus 7, and poliovirus 1 was examined in samples of surface freshwater collected from five sites of physically different character. these were an artificial lake created by damming a creek, a small groundwater outlet pond, both a large- and a medium-sized river, and a small suburban creek. survival was studied at temperatures of -20, 1, and 22 degrees c. the average amount of viral inactivation was 6.5-7.0 log10 ... | 1989 | 2545321 |
| coxsackie b3 virus encephalitis in a patient with agammaglobulinaemia. | a 19-month-old boy with agammaglobulinaemia contracted a coxsackie b3 virus infection which caused meningoencephalitis. his neurological status deteriorated over the following 4 years and he died aged 6 years. treatment with gammaglobulin i.m. and igg-fab2 fragments i.v. was not effective. enterovirus can cause life-threatening infections in immunodeficient patients. | 1989 | 2545452 |
| prolonged duck hepatitis b virus replication in duck hepatocytes cocultivated with rat epithelial cells: a useful system for antiviral testing. | duck cultured hepatocytes from pekin ducks naturally infected by duck hepatitis b virus can remain functional twice longer if a coculture system with rat liver epithelial cells is used instead of ordinary primary culture. the use of a selective medium in which ornithine and lactate replaced arginine and glucose, respectively, allowed viral replication initiated in vivo to be maintained in the coculture for 2 months. several antiviral compounds including the pyrophosphate analog (phosphonoformic ... | 1989 | 2545588 |
| characterization of b lymphocytes present in the demyelinating lesions induced by theiler's virus. | theiler's virus, a murine picornavirus, persists in the central nervous system of sjl/j mice and causes inflammation and demyelination in the white matter of spinal cord. we isolated inflammatory cells from the central nervous system of infected animals and studied their functions in vitro. flow microfluorimetry analysis showed the presence of all major lymphocyte subsets, namely cd4+ and cd8+ t cells as well as b lymphocytes. b lymphocytes were activated in vitro and the antigenic specificity o ... | 1989 | 2545781 |
| chimeric picornavirus polyproteins demonstrate a common 3c proteinase substrate specificity. | cross-species proteolytic processing was demonstrated by the 3c proteinases of human rhinovirus 14 and coxsackievirus b3 on poliovirus-specific polypeptide precursors. chimeric picornavirus cdna genomes were constructed in a t7 transcription vector in which the poliovirus 3c coding region was substituted with the corresponding allele from human rhinovirus 14 or coxsackievirus b3. in vitro translation and processing of the polypeptides encoded by the chimeric genomes demonstrated that the proteol ... | 1989 | 2545915 |
| a serologic survey of encephalomyocarditis virus infection in pigs in italy. | neutralizing antibodies to encephalomyocarditis virus (emcv) were found in 69.13% of the serum samples obtained from pigs from representative regions of italy. the antibodies are distributed fairly uniformly throughout the swine populations with all age groups being equally involved. | 1989 | 2546020 |
| viral heart disease. | | 1989 | 2546260 |
| role of natural killer cells in experimental murine myocarditis. | | 1989 | 2546262 |
| role of cytotoxic t cells in experimental myocarditis. | | 1989 | 2546263 |
| anti-viral and anti-myocyte antibodies in experimental myocarditis. | | 1989 | 2546264 |
| hepatitis a virus hemagglutination and a test for hemagglutination inhibition antibodies. | like enteroviruses, hepatitis a virus (hav) hemagglutinated various species of erythrocytes under similar conditions. hav-specific antibodies in both acute- and convalescent-phase sera were found to inhibit hemagglutination. the hav hemagglutination inhibition test can be used for diagnosis, epidemiological surveillance, and vaccine assessment. | 1989 | 2546979 |
| detection of neutralizing igm antibodies in the diagnosis of enterovirus infections. | data are presented of igm detection by a neutralization test used routinely in 1,062 patients. antigens isolated during the period of investigation were ev4, ev7, ev11, ev18, ev21, ev24, ev33, ca9, cb2, cb4, and cb5. no difference was observed in the distribution of igm-positive sera according to age and sex. total antibodies are at higher titres when igm antibodies are present. polytypic igm responses are not frequent (less than 10%). the frequency of the igm-positive sera for a given serotype ... | 1989 | 2547024 |
| new model for the secondary structure of the 5' non-coding rna of poliovirus is supported by biochemical and genetic data that also show that rna secondary structure is important in neurovirulence. | a secondary structure model for the 5' non-coding rna of poliovirus has been derived by comparing computer-generated folding patterns of equivalent sequences from a number of related enteroviruses and rhinoviruses and identifying compensating mutations that suggest conservation of a common secondary structure. although certain elements are similar, the new model differs considerably from a previously published minimal energy structure and is consistent with the observed sensitivity of in vitro r ... | 1989 | 2547075 |
| human infection with encephalomyocarditis virus in new south wales. | | 1989 | 2547141 |
| coxsackie virus and urogenital pathology. | in 43 women (average age 49.6 years), and 70 men (average age 55.2 years) with pathology of the genito-urinary apparatus, seroantibodies to coxsachie virus b were measured using the passive hemoagglutination method and the virus was isolated in the uterine. viral isolation test was negative in all urine samples tested. seropositivity for coxsackie virus was reported in 26 women (60.46%) and in 51 men (72.85%). positivity to b1 was 37.16% (42 cases), b2 in 38.05% (43 cases), and to b4 in 35.39% ( ... | 1989 | 2547535 |
| [an etiological study of acute hemorrhagic conjunctivitis in beijing area in 1984]. | acute hemorrhagic conjunctivitis (ahc) has been widespread in china since 1971. an etiological survey of the sporadic outbreak in beijing was done in 1984. conjunctival swabs and sera were collected at the eye clinic of peking union medical college hospital and 10 strains of virus were isolated from 46 cases by culture on the hela cell line. 8 strains were identified as enterovirus 70 by homologous antiserum j 670/71 obtained from the california state laboratory of viral and rickettsial diseases ... | 1989 | 2547550 |
| [changes in the myocardium in encephalomyocarditis of pigs]. | the myocardial lesions of pigs (age from 2.5 to 3.5 month) with encephalomyocarditis virus infection are presented. the disease is caused by members of the genus cardiovirus (family picornaviridae). in greece it was first observed 1987 in two stables of pig-breeders with a mortality of 80%. the macroscopic changes are characterized by a myocardial degeneration with greyish pale linear or round foci and by an accumulation of a clear red to yellow fluid in the pericardium and the pleural cavity. t ... | 1989 | 2547556 |
| monoclonal anti-idiotypic antibodies regulate the expression of virus-induced murine myocarditis. | a monoclonal anti-idiotypic antibody (anti-id), produced by electrofusion and designated anti-id88, was able to modulate expression of murine autoimmune myocarditis mediated by coxsackievirus b3 (cvb3). the anti-id was characterized as an immunoglobulin g2b species possessing kappa light chains and was able to reduce expression of inflammatory myocarditis in anti-id-pretreated mice challenged with cvb3. anti-id88 was able to stimulate specific cell-mediated immunity against anti-id88, as well as ... | 1989 | 2547722 |
| factors affecting virus plaque confirmation procedures. | direct plaque counts obtained by using the monolayer cell culture assay technique reliably confirmed the number of viruses isolated. analysis revealed some significant differences in the false-positive rate, depending on the test method used or virus samples evaluated. plaques from laboratory stock viruses showed a higher confirmation rate than sewage plaque isolates. test results with laboratory stock viruses suggested that confirmation rates may be affected by virus types present in the sample ... | 1989 | 2547809 |
| enterovirus igm detection: specificity of mu-antibody-capture radioimmunoassays using virions and procapsids of coxsackie b virus. | a predominantly type-specific mu-capture radioimmunoassay (ria) of igm antibodies to coxsackie b1-b5 (cb1-cb5) viruses was previously described (frisk et al., 1984). the present study is concerned with the specificity of this assay, using as antigen different strains of one serotype (cb5) and procapsids of two serotypes (cb3 and cb5). eight strains of cb5 virions were tested against acute and/or convalescent sera from 10 patients from whom cb5 had been isolated. seven patients' sera were tested ... | 1989 | 2547815 |
| a capture antibody elisa for detection of antibodies against bovine enterovirus. | a capture antibody elisa was developed for detecting antibodies against bovine enterovirus. chicken igg f(ab')2 fragments against strain j2129 captured virus effectively. the captured virus was used to detect specific antibodies against the virus in bovine sera. the specificity of the elisa is high. the factors affecting the specificity are discussed. the assay is more sensitive and economic than traditional serum neutralisation. | 1989 | 2547816 |
| fatal rhabdomyolysis and renal failure associated with hand, foot and mouth disease. | fatal severe rhabdomyolysis and anuric renal failure developed in a young man who had contracted hand, foot and mouth disease during an epidemic of this disease. viral studies implicated coxsackievirus a16 as the infecting agent. this appears to be the first reported case of rhabdomyolysis that was associated with hand, foot and mouth disease and infection with coxsackievirus a16. | 1989 | 2548069 |
| enterovirus surveillance--united states, 1989. | | 1989 | 2548074 |
| coxsackievirus b3-induced acute pancreatitis: analysis of histopathological and viral parameters in a mouse model. | coxsackievirus b3 infection in mice was studied histopathologically, by virus isolation and by nucleic acid hybridization after intraperitoneal inoculation of the virus. extensive viraemia was detected for 1-3 days post-infection. all mice developed necrotizing acute pancreatitis and focal myocarditis. pancreatitis eventually lead to complete atrophy of the exocrine pancreas. however, the islets of langerhans and pancreatic ducts remained morphologically intact. virus could be demonstrated in pa ... | 1989 | 2548562 |
| characterization of the immune response to intracerebral inoculation of theiler's murine encephalomyelitis virus: fine protein specificity of the splenic t cell and humoral antibody response. | the spleen t cell proliferative response of sjl/j mice primed by intracerebral inoculation of theiler's murine encephalomyelitis virus (tmev) was characterized in vitro. a dose-response [3h]thymidine incorporation was found with tmev, but not with unrelated viruses. the maximum response was obtained at doses of 5 x 10(5) cells per well with cultures of 96 h duration. viral capsid proteins vp1 and vp2 carried the antigenic determinants for the t cell response, vp3 playing no role in the in vitro ... | 1989 | 2548954 |
| epidemiology and laboratory diagnosis of infection with viral and bacterial pathogens in infants hospitalized for suspected sepsis. | a prospective study was conducted to determine the frequency and distribution of bacterial and viral pathogens in infants hospitalized with suspected sepsis and to evaluate the potential of virus detection for improving patient management. a causative organism was detected in 157 (67%) of 233 previously healthy infants less than 3 months of age, who had been hospitalized for suspected sepsis: 19 (8%) had bacterial infections, 135 (58%) had viral infections, and 3 (1%) had mixed viral-bacterial i ... | 1989 | 2549231 |
| from the centers of disease control. enterovirus surveillance--united states, 1989. | | 1989 | 2549277 |
| use of synthetic oligodeoxyribonucleotides for type-specific identification of coxsackie b viruses. | synthetic oligodeoxyribonucleotides were used for type-specific identification of members of the coxsackie b virus group by nucleic acid hybridization. two pairs of oligonucleotide chains were constructed based on nucleotide sequences in the vp1 regions of coxsackieviruses b3 and b4. each labelled probe had a length of 24 nucleotides. the results showed that the oligonucleotide hybridized in a type-specific manner when assayed with extracts from cells infected with all different coxsackie b viru ... | 1989 | 2549405 |
| outbreak of herpangina associated with coxsackievirus b3 infection. | an outbreak of acute febrile illness was observed in summer, 1987, in a welfare home in which 31 healthy infants were accommodated. within a 5-day period 25 infants (81%) acquired a febrile illness. coxsackievirus b3 was isolated from 16 (64%) of 25 throat swabs. in the patients in whom viral culture was negative or not performed, 6 were serologically identified as having a coxsackievirus b3 infection. among 22 patients identified as having a coxsackievirus b3 infection 7 had typical herpangina ... | 1989 | 2549494 |
| [virologic research on the quality of 2 sources of drinking water from zone "i"]. | enteroviruses were isolated from 4.6% out of the examined tap water samples. no relation could be established between viral pollution and classic quality indexes of water. | 1989 | 2549702 |
| [the morphology of coxsackie b virus infections of the nervous system]. | neuropathological studies were conducted into 20 virologically secured coxsackie-b virus infections of infants and adults, with the nervous system being involved. inflammatory processes affect both gray and white cerebral regions as well as the peripheral nervous system, and in adulthood they may take the form of severe necrotising encephalitis. necroses of that kind may be recordable from various regions of the central nervous system. notwithstanding tempestuous progress in the development of m ... | 1989 | 2549750 |
| evidence for a novel picornavirus in human dermatomyositis. | we prepared rna probes from cloned segments of human and murine enteroviruses (evs) for in situ hybridization of skeletal muscle biopsies from patients with dermatomyositis (dm), polymyositis, other inflammatory myopathies, and noninflammatory muscle diseases, and from normal control subjects. a probe derived from theiler's murine encephalomyelitis virus (tmev) detected viral rna within mononuclear cells of the interstitial connective tissue in 3 of 5 patients with adult-onset dm. none of these ... | 1989 | 2549850 |
| diagnosis of a field outbreak of avian encephalomyelitis by enzyme-linked immunosorbent assay. | | 1989 | 2549931 |
| [effect of astragalus membranaceus on electrical activities of coxsackie b-2 virus-infected rat myocardial cells in culture]. | beating myocardial cell cultures of neonatal rats were prepared in vitro and infected with coxsackie b-2 virus. the cells were evaluated in the post-infected period for changes in beating percentage and cytopathic effect (cpe), alterations in the electrical activities by standard microelectrode techniques, and the protective effect of astragalus membranaceus (am) on coxsackie b-2 virus-infected neonatal rat myocardial cell cultures was observed. the beating percentage began to decrease in the in ... | 1989 | 2550160 |
| enteroviral-related antigen in circulating immune complexes of amyotrophic lateral sclerosis patients. | circulating immune complexes were isolated by polyethylene glycol precipitation from the sera of patients with amyotrophic lateral sclerosis. rabbits immunized with circulating immune complexes from 3 of 5 amyotrophic lateral sclerosis patients induced antisera that specifically reacted with enterovirus-infected cells by immunofluorescence and enzyme-linked immunosorbent assay. these antisera were nonneutralizing and did not react with purified virus. in addition, peripheral lymphocytes of amyot ... | 1989 | 2550387 |
| detection of enteroviruses using cdna and synthetic oligonucleotide probes. | this study compares the detection of enterovirus rna by cdna probes prepared from both the 5' and 3' end of the genome of coxsackie a21 and b4 with the use of synthetic oligonucleotides prepared from short but highly conserved sequences in the 5' end non-coding region of the picornavirus genome. the cdna probes detected enteroviruses with a variable level of sensitivity which presumably depended on the degree of genomic homology with the detecting probes. generally probes from coxsackievirus a21 ... | 1989 | 2550504 |
| coxsackievirus-b3-induced myocarditis: virus receptor antibodies modulate myocarditis. | two variants of coxsackievirus group b, type 3 (cvb3) differ in ability to induce myocarditis in balb/ccum mice. infection with the highly pathogenic variant (cvb3m) stimulates autoimmunity to normal cardiocyte antigens, and tissue injury results primarily from an autoreactive cytolytic t lymphocyte (actl). animals infected with the less pathogenic cvb3o variant do not develop actl, although cvb3o replicates to high titers in the heart and polyclonal neutralizing antisera fail to distinguish bet ... | 1989 | 2550544 |
| acid stability of hepatitis a virus. | the acid stability of unpurified and highly purified hepatitis a virus (hav) was tested and compared with that of poliovirus type 1, coxsackievirus types a9 and b1 and echovirus type 9. only hav had a high residual infectivity after 2 h of exposure to ph 1 at room temperature, remaining infectious for up to 5 h. at 38 degrees c, ph 1, hav remained infectious for 90 min. highly purified hav was found to be infectious for 8 h at ph 1 and room temperature. this indicates that the increased stabilit ... | 1989 | 2550575 |
| immunity in pekin ducks experimentally and naturally infected with duck hepatitis b virus. | the immune response to duck hepatitis b virus (dhbv) had not been elucidated. an assay was therefore established to detect the presence of antibody to dhb surface antigen (anti-dhbs) in serum of experimentally inoculated and naturally infected ducks. anti-dhbs in serum was detected by indirect ria from the percentage inhibition of binding of rabbit anti-dhbs to purified dhbsag. specificity was confirmed by positive and negative controls, infected and noninfected sera, and a mouse monoclonal anti ... | 1989 | 2550583 |
| treatment of encephalomyocarditis virus-induced central nervous system demyelination with monoclonal anti-t-cell antibodies. | infection of balb/c mice with the m variant of encephalomyocarditis virus resulted in the development of a paralytic syndrome in 7 to 10 days. the paralysis was maximal during the period of viral clearance; most of the animals recovered from the initial deficit and showed no delayed recurrences. pathologically, the white matter of brain and spinal cord showed well-demarcated areas of perivascular cuffing, demyelination, and, during recovery, remyelination by oligodendrocytes--all suggestive of p ... | 1989 | 2550666 |
| [use of direct calorimetry for continuously following the course of experimental infections in the mouse]. | to obtain information about early symptoms of infection measurements of heat production were performed in experimentally infected animals. as a model mengo virus-infected mice were used. construction and physical properties of the flow calorimeter device are described. fever was not detected by method used. however, deviations from normal behaviour of the animals were demonstrated as a result of the relationship between motoric activity and heat produced. furthermore, the method may be particula ... | 1989 | 2551106 |
| absence of effects of cyclosporine on myocardial lymphocyte subsets in coxsackievirus b3 myocarditis in the aviremic stage. | to test the therapeutic efficacy of immunosuppression with cyclosporine upon the aviremic stage of coxsackievirus b3 (cb3) myocarditis, 2-week-old balb/c mice were inoculated with 3 x 10(2) plaque-forming units of cb3, and the effects of cyclosporine on peripheral, splenic, and myocardial lymphocyte subsets were investigated. cyclosporine, 25 mg/kg/day, was administered subcutaneously daily on days 10-31 (experiment 1) and days 30-51 (experiment 2). treated groups were compared with infected con ... | 1989 | 2551533 |
| coxsackievirus b3 myocarditis in c3h/hej mice: description of an inbred model and the effect of exercise on virulence. | the effect of forced exercise on the development of coxsackievirus b3 myocarditis in inbred c3h/hej mice was studied. four groups of mice (30 per group) were formed: infected-exercised (group i); infected-unexercised (group ii); uninfected-exercised (group iii); and uninfected-unexercised (group iv). infected mice were inoculated intraperitoneally with 1.0 x 10(2.1) tcid50 coxsackievirus b3. exercised animals were swum daily for 60 minutes on days 1-9. myocardial viral titers were acutely elevat ... | 1989 | 2551723 |
| [behavior of enteroviruses in the soil]. | | 1989 | 2551789 |
| [photosensitive paper as an indicator of the virucidal effect of bactericidal lamps]. | | 1989 | 2551790 |
| [acute idiopathic pleuropericarditis associated with transitory hypoparathyroidism]. | | 1989 | 2552234 |
| fatal coxsackie b1 virus infection in neonates. | | 1989 | 2552390 |
| [macrophage disappearance reaction in mice during an infection caused by coxsackie b3 virus]. | intraperitoneal inoculation of 2 1/2-3-month-old male balb/c mice with coxsackie b3 virus in a dose of 10(7.2) tcd50 was demonstrated to be accompanied by inhibition of the starch-induced exudation of leukocytes into the peritoneal cavity. a decrease in the number of macrophages was observed in 3-day exudates induced at 4 and 21 days but not at 0, 7, and 14 days of infection. the macrophage disappearance reaction showed that the intraperitoneal inoculation of the challenge virus dose (10(7.2) tc ... | 1989 | 2552686 |
| recovery of solid-associated virus: evaluation of different procedures. | three different extraction-concentration methods for virological analysis of sludge, marine and river sediments are studied. enterovirus present in the concentrates were enumerated using the plaque technique with bgm cells. the results show that the indirect extraction technique isolated virus from the largest number of marine sediment samples. the direct extraction method is the best to recover virus from river sediments. among the sludge samples, the number of positive samples did not vary muc ... | 1989 | 2553039 |
| [retinoic acid modification of cell culture used for reproduction of enteropathogenic viruses]. | the 0.001-0.005% retinoic acid injection into the growth cultural medium of prime and continue cell cultures 12-24h before inoculation considerably raised the cell sensitivity to animal entero- and coronaviruses. the entero- and coronaviruses concentrations in cultural medium increased by 10(1.58) and 10(1.68)tcid 50/1.0 respectively. the optimized parameters of the cell culture processing for the enteropathogenic viruses reproduction improvement are proposed. | 1989 | 2553149 |
| end-stage dilated cardiomyopathy. persistence of enterovirus rna in myocardium at cardiac transplantation and lack of immune response. | tissue from the explanted hearts of 21 patients with idiopathic dilated cardiomyopathy or 19 patients with other specific heart muscle diseases were investigated for presence of enterovirus-specific rna with an enterovirus group-specific cdna probe. this was complementary to coxsackievirus b2 rna sequences between nucleotide numbers 6,550 and 7,400, which are highly conserved between enteroviruses. hearts from six patients with dilated cardiomyopathy and one patient with ischemic heart disease w ... | 1989 | 2553297 |
| susceptibility of four species of small rodents to encephalomyocarditis (emc) virus infection. | the d variant of encephalomyocarditis virus (10(1)-10(5) pfu/head) was intraperitoneally inoculated into 4 species of small rodents, rats, mice, syrian hamsters, and mongolian gerbils, and the susceptibility of these animals to emc virus was examined virologically and histopathologically 3 days after infection. viral replication was detected in the brain (mice), in the heart (mice and gerbils), and in the pancreas (mice, hamsters, and gerbils). no viral replication was detected in rats. histopat ... | 1989 | 2553452 |
| relation between aeromonas and faecal coliforms in fresh waters. | a possible correlation between the presence of mesophilic aeromonads and the number of faecal coliforms present in three fresh-water habitats subject to differing levels of faecal pollution was investigated. concentration of aeromonas spp. between 10(2)-10(9) cfu/100 ml and faecal coliforms of between 9-10(7) cfu/100 ml were found in the waters. in water free from faecal pollution there was no correlation but in polluted waters there was a significant relationship between the numbers of aeromona ... | 1989 | 2553653 |
| epidemiological observation of coxsackieviruses group b in sewage. | different types of coxsackieviruses group b were isolated from the sewage consecutively annually from january 1982 to march 1987: type 3 in 1982, type 4 in 1983, type 2, 4 and 5 in 1984, type 3 in 1985, and type 4 in 1986 were found. these viruses caused large epidemics among children aged 1 to 4 and proximities. herald waves for the epidemics were observed six times during this period, and the viruses were detected in a brief period of isolation. with respect to the periodicity of these viruses ... | 1989 | 2553831 |
| intrathecal synthesis of virus-specific oligoclonal antibodies in patients with enterovirus infection of the central nervous system. | cerebrospinal fluid (csf) and serum samples from six patients with enterovirus infections were investigated by isoelectric focusing (ief) and affinity-mediated immunoblot ami) for the clonal distribution of entervirus-specific antibodies. in two patients with either acute meningitis or encephalitis and in one patient with a relapse of multiple sclerosis, oligoclonal igg bands specific for enteroviruses were found predominantly in the csf, revealing intrathecal synthesis of these antibodies. in t ... | 1989 | 2553877 |
| [clinical aspects of viral meningitis in children]. | | 1989 | 2554245 |
| a temperature-sensitive defect of enterovirus 70 is located at the uridylylation of the genome-linked protein vpg in vitro. | the temperature-sensitive phenotype of enterovirus 70 (ev70) was examined by use of an in-vitro rna replication system derived from a membrane fraction (crude replication complex, crc) of ev70-infected hela cells. this system was capable of synthesizing the nucleotidyl proteins vpg-pu and vpg-pupu. formation of these nucleotidyl proteins was completely abolished when the in-vitro reaction was performed at the nonpermissive temperature for virus replication. considering our previous observation t ... | 1989 | 2554583 |
| concentration of giardia lamblia cysts, legionella pneumophila, clostridium perfringens, human enteric viruses, and coliphages from large volumes of drinking water, using a single filtration. | poliovirus, coliphages, giardia lamblia cysts, clostridium perfringens spores, and legionella pneumophila were concentrated simultaneously in a single pass by sequential filtration of large volumes of drinking water through 3- and 1-micron wound electronegative fiberglass cartridge filters (25.4 cm). filtration was performed under acidic conditions (ph 3.5) in the presence of 0.001 m aluminum chloride to enhance adsorption. elution of all the microorganisms entrapped or adsorbed to the filters w ... | 1989 | 2555036 |
| a theoretical study of the acidification of the rhinovirus capsid. | electrostatic calculations for human rhinovirus 14 indicate that histidine-base residue pairs in the region of a beta-strand interaction between pentamers may be involved in a ph-induced process that leads to the release of viral rna. other picornavirus sequences are examined for these residue pairs, a subset of which is present in enteroviruses. foot and mouth disease virus possesses one of the residue pairs, and cardioviruses, which undergo a separate ph and halide ion-induced capsid dissociat ... | 1989 | 2555222 |
| tropism and histopathology of the d, b, k, and mm variants of encephalomyocarditis virus. | variants of encephalomyocarditis virus (emcv), which are immunologically indistinguishable by hyperimmune serum, produce different disease syndromes in mice. for instance, in icr swiss male mice, emcv-b produces no overt illness, emcv-mm produces severe neurological signs followed by death, emcv-d destroys pancreatic beta cells producing a disease syndrome resembling insulin-dependent diabetes mellitus, and emcv-k is lethal but produces no overt signs of infection. the present study was done to ... | 1989 | 2555446 |
| polyprotein processing of theiler's murine encephalomyelitis virus. | to investigate polyprotein processing of theiler's murine encephalomyelitis viruses, we analyzed in vitro translation reactions programmed by in vitro-derived transcripts from an infectious full-length cdna clone of the da strain of theiler's virus. to help identify the proteinases that carried out the processing, we modified the da cdna clone transcription template by linearization with different restriction endonucleases that generate templates of different lengths or by constructing linker in ... | 1989 | 2555559 |
| infectious cdna clones of the da strain of theiler's murine encephalomyelitis virus. | the da strain and other members of the to subgroup of theiler's murine encephalomyelitis viruses cause a persistent demyelinating infection, whereas the gdvii strain and other gdvii subgroup strains cause an acute lethal polioencephalomyelitis. we generated an infectious da cdna clone inserted into a transcription vector. virus derived from transfection of transcripts produced a demyelinating disease indistinguishable from that of wild-type virus. the infectious clone provides a critical reagent ... | 1989 | 2555569 |
| general characteristics and viral susceptibility of a newborn pig kidney (npk) continuous culture. | we have developed a fibroblastic-like continuous culture of newborn pig kidney (npk). the current cell line was serially passaged 160 times and appeared to be well suited for production and assay of a number of viruses affecting pigs, such as pig parvovirus, pseudorabies and transmissible gastroenteritis. the cell line appeared aneuploid, with a modal chromosome number of 36 and induced tumors, classified as fibrosarcoma, in athymic mice. | 1989 | 2555655 |
| unusual development of polyoma virus in the brains of two patients with the acquired immune deficiency syndrome (aids). | two hiv-positive male patients presented with a variety of symptoms including hemiparesis, unsteadiness, progressive loss of vision and poor memory. there were multiple non-enhancing lesions shown by ct scan in the white matter of the cerebral hemispheres. specimens obtained by burr-hole biopsy showed the features of progressive multifocal leucoencephalopathy (pml) in both cases. electron microscopy demonstrated round and rod shaped particles of papovavirus in the nuclei and cytoplasm of oligode ... | 1989 | 2555730 |
| potential use of nonpathogenic enteroviruses for control of human disease. | until recently, it has been generally assumed that all human viruses are causative agents of diseases and should be regarded as harmful pathogenic agents that require control measures. in the early 1950s we began to doubt this view. in the course of experiments on virus isolations from feces of normal children, as well as in studies of isolates from animals and from tissue cultures, data accrued which suggested that some conditionally pathogenic and some completely nonpathogenic strains of enter ... | 1989 | 2555836 |
| an epidemic of acute hemorrhagic conjunctivitis in american samoa caused by coxsackievirus a24 variant. | between may 25 and july 5, 1986, an epidemic of acute hemorrhagic conjunctivitis affected an estimated 47% of the population on american samoa. coxsackievirus a24 variant was isolated from 18 of 22 patients. this is the first documented outbreak of acute hemorrhagic conjunctivitis due to coxsackievirus a24 variant outside of southeast asia and the indian subcontinent. when this outbreak was compared with an outbreak on the island in 1981-1982 caused by enterovirus 70, conjunctival hemorrhage or ... | 1989 | 2556026 |
| the swedish childhood diabetes study iii: igm against coxsackie b viruses in newly diagnosed type 1 (insulin-dependent) diabetic children--no evidence of increased antibody frequency. | sera from essentially all swedish children aged 0-14 years with type 1 (insulin-dependent) diabetes mellitus with onset during an autumn period (october-december 1985) and a late spring period (may-june 1986) were selected. in all, 98 patients were analysed for igm antibodies against coxsackie b virus serotypes 1 through 5 by a mu-antibody capture radio immunoassay technique. sera from 94 referent children matched for age, sex and residential area, collected during the same period, were also ana ... | 1989 | 2556308 |
| [effect of cation flocculants on enterobacteriaceae and enteroviruses]. | | 1989 | 2556332 |
| coxsackie b5 papillitis. | | 1989 | 2556502 |
| recovery of viruses and bacteria in waters off bondi beach: a pilot study. | a pilot study was conducted between february and april, 1989, on the occurrence of sewage-derived viruses and bacteria in the beach and nearshore waters off bondi, sydney. enteroviruses were isolated from 41% of a total of 66 sewage, sea-water, grease and sediment samples. poliovirus vaccine strains accounted for 78% of the isolates. adenoviruses were isolated four times and coxsackievirus b was isolated twice in samples that were collected away from the bathing area. rotavirus and hepatitis a v ... | 1989 | 2556634 |
| enterovirus-specific igm in the diagnosis of meningitis. | samples of serum from 557 patients with a clinical diagnosis of meningitis or encephalitis and referred to the epsom public health laboratory during a period of 3 years were tested for enterovirus-specific igm in a mu capture enzyme-linked immunosorbent assay (elisa). enterovirus-specific igm was detected in 45% samples from all age groups. in the 3-5-year age group, 67% specimens were positive. a notable male predominance (73%) was seen in the age group 0-15 years. as predicted, a seasonal incr ... | 1989 | 2557369 |
| chronic coxsackie b infection mimicking primary fibromyalgia. | we describe a patient with primary fibromyalgia according to smythe's criteria who had evidence of chronic infection with coxsackie virus over a 4-year period. we discuss the association of coxsackie infection and myalgic syndromes and review the literature. | 1989 | 2557447 |
| hemophagocytic syndrome associated with coxsackie virus a 9 infection in a non-immunosuppressed adult. | | 1989 | 2558130 |
| polymerase chain reaction for human picornaviruses. | we have used enzymic amplification of specific nucleic acid sequences followed by hybridization, for the rapid detection and typing of human picornaviruses after cell culture isolation. the test is based on the synthesis of cdna, the polymerase chain reaction and the use of oligonucleotide probes. the primers were selected from the 5' non-coding region of the genome representing highly conserved regions. sequences specific to enteroviruses and rhinoviruses were used as probes. the assay was able ... | 1989 | 2558157 |
| the nucleotide sequence of coxsackievirus a9; implications for receptor binding and enterovirus classification. | the complete nucleotide sequence of the genome of coxsackievirus a9 (cav-9) has been determined from cdna cloned in escherichia coli. excluding the 3' poly(a) stretch, the rna genome is 7452 nucleotides long and encodes a single polyprotein of 2201 amino acids. comparison of the nucleotide and predicted amino acid sequences with those of the coxsackieviruses b1, b3 and b4 reveals a surprising degree of homology, with overall amino acid homologies of 86.9%, 86.2% and 87.0%, respectively. in contr ... | 1989 | 2558158 |
| explants of human oral epithelium exposed to viruses and cancer chemotherapeutics. | cultures of proliferating epithelial cells were established from explants of normal human oral epithelium from healthy young volunteers. the epithelial cells were found permissive for herpes simplex virus type 1 and type 2, coxsackie virus a-4 and a-16, adenovirus type 5, measles vaccine, rubella and influenza type a virus-. medium from deae-pretreated epithelial cultures infected with two subtypes of human immunodeficiency virus-1 showed an increasing content of virusprotein with time by antige ... | 1989 | 2558179 |
| [the hemagglutinating properties and adsorption on erythrocytes of coxsackie b-3 viruses and their selected bentonite variants]. | the paper is devoted to the study of the coxsackie b-3 viruses and their bentonite variants passaged on the primary and transplanted cell cultures for their hemagglutinating (ha) properties and a degree of adsorption on erythrocytes. it is shown possible to detect the ha activity of the coxsackie b-3 viruses which are passaged on the transplanted cell cultures: the variant abent has the hemagglutinating activity relative to human and rabbit erythrocytes. the variant abent+ of the coxsackie b-3 v ... | 1989 | 2558279 |
| [a method for detecting the hemagglutinating activity of enteroviruses]. | optimal conditions to reveal the hemagglutinating activity in the coxsackie viruses of group b and hemagglutination test (hat) performed by the micromethod are studied and determined. the titre of hemagglutinins revealed in hat is established to depend on ph of the phosphate-buffer solution, concentration of human erythrocytes and their group attribution. the viruses passaged at the suboptimal temperature 33 degrees c are shown to possess the maximal hemagglutinating activity. | 1989 | 2558280 |
| [study of the antiviral activity of nonsteroidal anti-inflammatory agents in cell culture]. | | 1989 | 2558448 |
| isolation of marine bacteria with antiviral properties. | we report in this study the isolation of marine bacteria with antiviral properties that have been tentatively classified as moraxella. these bacteria retained their virucidal capacity after prolonged subcultivation in the laboratory. the virus-inactivating agent could not be separated from the viable marine bacteria, indicating that the active agent(s) either remains associated to the microorganisms or has a very short lifetime, or both. the antiviral capacity of the isolated microorganisms was ... | 1989 | 2558789 |
| analysis of virus protein heterogeneity among group b coxsackie viruses using a "mini" two-dimensional gel electrophoresis system. | the application of a small format two-dimensional polyacrylamide gel electrophoresis (2d-page) system to the study of protein heterogeneity among group b coxsackie virus (cvb) isolates is described. under the conditions of electrophoresis developed during this study, protein samples could be processed within 7 h and up to 300 intracellular proteins were resolved from uninfected hep-2 cell lysates. 2d-page was used to characterise the intracellular proteins of clinical cvb isolates of serotypes 4 ... | 1989 | 2558885 |
| [dilated cardiomyopathy: sequela of viral myocarditis?]. | the study was undertaken to examine 100 patients with dilated cardiomyopathy (dcm) and 62 with viral myocarditis (vm). serological studies revealed that 60% of the vm patients and 25% of the dcm patients had diagnostically significant antibody titers to coxsackie b virus. the patients from the two groups displayed nk cell deficiency, quantitative and functional suppressor defect, and severe derangements in the system of interferons. unlike dcm patients, some vm patients showed greater quantities ... | 1989 | 2559232 |
| identification of human picornaviruses by nucleic acid probes. | human picornaviruses include rhinoviruses and enteroviruses which are responsible for both common and severe clinical diseases. rhinoviruses are a frequent cause of respiratory infections while members of enterovirus subgroups, polio, coxsackie and echo viruses are often responsible for infections of the central nervous system, myocarditis, myositis etc. human picornaviruses consist of nearly two hundred serotypes and therefore their specific identification after virus isolation, or the diagnosi ... | 1989 | 2559319 |
| solution hybridization and enzyme immunoassay for biotinylated dna-rna hybrids to detect enteroviral rna in cell culture. | a non-isotopic hybridization assay is described for detection of enteroviral rna in cell culture. two biotin-labelled cdna probes, corresponding to 1 kb from the 5' end and 3.5 kb from the 3' end of the coxsackievirus b3 genome, were hybridized in solution with protease and detergent-treated cell culture suspensions. labelled dna-rna hybrids were captured on microtiter plates coated with anti-biotin antibody and bound hybrids were measured with a beta-galactosidase-labelled monoclonal antibody s ... | 1989 | 2559321 |
| [acute hemorrhagic conjunctivitis caused by a variant of coxsackievirus a24, in belém, pará, brazil, 1987]. | an epidemic of acute haemorrhagic conjunctivitis in belém, pará, brazil, was investigated. from 83 patients, 73 samples of virus was isolated in cultures of hep-2 cells from conjunctival swab and throat swab. the virus isolates were identified in centers for disease control, atlanta, georgia, usa, as an antigenic variant of coxsackievirus a24. neutralization test was done on 56 paired serum samples, using entero virus type 70 (ev70) and virus isolates. serological conversions for virus isolates ... | 1989 | 2559469 |
| in vitro and in vivo activities of win 54954, a new broad-spectrum antipicornavirus drug. | win 54954 (5-[5-[2,6-dichloro-4-(4,5-dihydro-2-oxazolyl)phenoxy]pentyl]-3- methylisoxazole) is a new member of the class of broad-spectrum antipicornavirus compounds known to bind in a hydrophobic pocket within virion capsid protein vp1. in plaque reduction assays, win 54954 reduced plaque formation of 50 of 52 rhinovirus serotypes (mics ranged from 0.007 to 2.2 micrograms/ml). a concentration of 0.28 microgram/ml was effective in inhibiting 80% of the 52 serotypes tested (ec80). win 54954 was a ... | 1989 | 2559655 |
| effect of 3'-azido-3'-deoxythymidine on replication of duck hepatitis b virus in vivo and in vitro. | 3'-azido-3'-deoxythymidine (azt) inhibits the replication of the human immunodeficiency virus (hiv) by blocking the formation of the phosphodiester bond and has been used clinically for the treatment of hiv infection. to assess the effect of azt on the replication of hepadnaviruses, which replicate through reverse transcription, both the liver tissue and primary cultured hepatocytes from ducklings previously infected with duck hepatitis b virus (dhbv) were examined for dhbv dna before and after ... | 1989 | 2559951 |
| pig erythrocyte ghost cells used for concentration of enteric viruses from experimentally contaminated clinical specimens. | a simple, rapid and efficient procedure of virus concentration from urine, cerebrospinal fluid (csf), and feces was developed. pig erythrocyte ghost cells were used to adsorb and elute such viruses as poliovirus lsc-1, echovirus 6, and human rotavirus (clinical isolate). in urine and csf, the adsorption efficiency range was 80-100% and elution was from 85% to greater than 100%. in addition, poliovirus lsc-1 was used as an experimental model to examine this procedure under various clinical condit ... | 1989 | 2559952 |
| molecular cloning of the complete genome of theiler's virus, strain da, and production of infectious transcripts. | we constructed a complete cdna clone of the genome of theiler's virus strain da in a bluescript plasmid. this recombinant plasmid, called ptmda, was used to synthesize full length rna transcripts of the viral insert. the rna was infectious for bhk cells. virus r1-da, obtained from transfected bhk cells, caused the biphasic disease classically observed with this strain of theiler's virus. sjl/j mice did not show clinical symptoms during the first week following intracranial inoculation, although ... | 1989 | 2560113 |
| anti-mouse igm immunoglobulin protects weanling mice from coxsackievirus infection. | mice treated from birth with rabbit anti-mouse igm antiserum (anti-mu), although immunosuppressed in regard to b cell function, may paradoxically be protected when challenged with certain viruses. our early studies indicated that short term treatment of weanling mice protected against semliki forest virus, herpes simplex virus, and coxsackievirus b1. for this study, the murine model of coxsackievirus b1 infection was chosen to determine if specific anti-mu immunoglobulin (anti-mu ig) would prote ... | 1989 | 2560114 |
| severity of disease induced by a pancreatropic coxsackie b4 virus correlates with the h-2kq locus of the major histocompatibility complex. | coxsackie b viruses are known etiological agents of pancreatic diseases, including diabetes. the pathogenesis of these infections is influenced by both host and viral factors. in this report, we examined whether the outcome of coxsackie b4 virus infection is dependent on the genes within the major histocompatibility complex (mhc). we generated a pancreatic variant, cb4-v and established an animal model system of pancreatitis with concurrent hypoglycemia in mice. infection of various b10 h-2 cong ... | 1989 | 2560295 |
| [effect of dexamethasone on coxsackie b-2 virus-infected rat beating heart cells in culture]. | the effects of dexamethasone (dex) on cultured rat beating heart cells infected with 100 tcid-50 coxsackie virus b-2 (cb2v) were observed. the beating % began to decrease in the infected group 2 or 3 d post-challenge. meanwhile, the cytopathic effect (cpe) appeared rapidly from 1+ to 3+. in the infected and dex-treated group 1 h after inoculation, the beating % and cpe in the whole flask were significantly higher and less, respectively, than that in the group infected (p less than 0.05) at the s ... | 1989 | 2560313 |
| acute non-haemorrhagic conjunctivitis due to coxsackievirus a24. | two cases of acute non-haemorrhagic conjunctivitis due to coxsackievirus a24 (ca24) are described. these are the first australian isolates. the presentation was as a severe conjunctivitis in otherwise healthy adults who had not travelled outside australia. the course was of short duration and self-limiting with no long-term sequelae. the isolates could not be neutralised by antiserum prepared against prototype ca24 but were identified by immune electron microscopy and complement fixation. | 1989 | 2560383 |
| viral contamination of some drinking waters in romania: a twenty years survey. | a synthesis is made on the 20 years virological survey of the drinking water from some towns of romania. the sampling of the water was made by the gauze-pad method. the virus concentration method by adsorption-elution with the yeast cells was applied concomitantly, at first with the pe60 method, then, during the last years, either with aluminum hydroxide or with the polymer pv methods, and the concentrates were inoculated both into suckling mice and into cell cultures. various types of coxsackie ... | 1989 | 2560479 |
| coxsackie virus a 24 variant as the etiological agent of the acute haemorrhagic conjunctivitis epidemic at vellore, in 1986. | coxsackie virus a 24 variant (ca24v) was found to be the etiological agent of an epidemic of acute haemorrhagic conjunctivitis (ahc) in vellore during the months of october and november in 1986. ca24v was isolated in 21 of 25 acutely ill patients. in addition, seroconversion was seen in 11 of the 16 patients from whom paired sera were collected. | 1989 | 2560770 |
| sex-dependent, early cytokine production by nk-like spleen cells following infection with the d variant of encephalomyocarditis virus (emcv-d). | spleen cell cultures from diabetes-resistant icr swiss females exhibited an increase in expression of ia antigens 24 hours post-infection (pi) with emcv-d while comparable spleen cell cultures from diabetes-susceptible males of this strain did not exhibit this increase in ia antigens expression. a monoclonal antibody specific for mouse interferon-gamma (ifn gamma) eliminated this increase in ia antigens expression. interferon-gamma (ifn gamma) and interleukin 2 (il-2) production by emcv-d-infect ... | 1989 | 2560916 |
| coxsackie virus and rheumatic fever. a correlative study. | twenty-one patients of whom 13 had acute rheumatic fever and 8 had recurrence of rheumatic fever were studied for the evidence of coxsackie b viral infection using neutralisation test. a significant rise was noted in 17 cases (81%) and two cases had very high initial titre of neutralising antibodies to coxsackie b viruses type 1 to 6. mixed infection with more than one serotype was seen in 11 cases. coxsackie b2 was the commonest type and 14 patients had antistreptolysin 'o' anti-bodies. the hig ... | 1989 | 2561125 |
| complete atrioventricular block associated with regional myocardial scarring in a patient with coxsackie b2 myocarditis. | transient atrioventricular (a-v) conduction abnormalities are often experienced in patients with evolving acute viral myocarditis, but persistent complete a-v block requiring permanent cardiac pacing is rare. we describe a case who developed irreversible complete a-v block during the long-term course of coxsackie b2 myocarditis. the endomyocardial biopsy revealed inflammatory cellular infiltrates and myocyte necrosis. a left ventriculogram and echocardiogram consistently demonstrated an aneurysm ... | 1989 | 2561163 |
| [the role of coxsackie b viruses in the etiology of dilated cardiomyopathy]. | in order to establish the relationship between coxsackie b virus infection and dilated cardiomyopathy (dcmp), 53 patients suffering from dcmp underwent serological tests. the rate of demonstration of virus-neutralizing antibodies at different titres was compared with the respective values in the previously examined 180 patients with myocarditis, 58 patients with myocardial sclerosis and 150 normal persons. in dcmp, antibodies against b2 type virus were detectable more frequently (p less than 0.0 ... | 1989 | 2561232 |
| [evaluation of 30-year-activity of the virology laboratory of the pasteur institute in madagascar (1958-1987)]. | the authors studied the different activities of pasteur institute virology laboratory since it was created. in general virology laboratory, 1.685 viral strains were isolated from 9.533 studied samples (17.6% of positivity). enterovirus represented 90% of the viral strains. from 2.417 taking of rhino-pharynx, 133 myxovirus were isolated: a/h3n2 represented 76% of them. 10.776 patients, from 36.169 antirabic department consultants, were vaccinated and 600 had an antirabic serotherapy. 2.828 suspec ... | 1989 | 2561255 |