| [the effect of 3'-azido-2',3'-dideoxythymidine on experimental viral infections]. | 3'-azido-2',3'-dideoxythymidine (az-t) inhibited effectively the reproduction of some retroviruses; among these viruses were the four serological subgroups of sarcoma raus virus in chicken embryo, avian myeloblastosis virus and erythroblastosis virus in chicken. this inhibition was specific towards retroviruses and practically was not observed in the case of infections dna- and rna-genome model viruses of vaccinia and influenza, at whose reproduction reverse transcriptase is not involved. three ... | 1987 | 2821379 |
| inhibition of retroviral protease activity by an aspartyl proteinase inhibitor. | retrovirus protease is an enzyme that cleaves gag and gag-pol precursor polyproteins into the functional proteins of mature virus particles. the correct processing of precursor polyproteins is necessary for the infectivity of virus particles: in vitro mutagenesis which introduces deletions into the murine leukaemia virus genome produces a protease-defective virus of immature core form and lacking infectivity. a therapeutic drug effective against disease caused by retrovirus proliferation could l ... | 1987 | 2821409 |
| the highly conserved amino-terminal region of the protein encoded by the v-myb oncogene functions as a dna-binding domain. | the retroviral oncogene v-myb encodes a 45,000 mr nuclear protein (p45v-myb) that is predominantly associated with the chromatin of transformed cells. it has previously been shown that p45v-myb, when released from chromatin by salt-treatment, binds to dna. to analyse the biochemical properties of p45v-myb in more detail we have expressed the v-myb coding region in escherichia coli. our results demonstrate that bacterially expressed myb protein has an intrinsic dna-binding activity. using two alt ... | 1987 | 2824190 |
| specific amino acid substitutions are not required for transformation by v-myb of avian myeloblastosis virus. | the protein product of the v-myb oncogene of avian myeloblastosis virus, p48v-myb, differs structurally in several ways from its normal cellular homolog, p75c-myb. we demonstrated that the 11 specific amino acid substitutions found in two independent molecular clones of this virus were not required for the transformation of myeloblasts by v-myb. | 1988 | 2828660 |
| [immunization against avian leukoses]. | | 1987 | 2831834 |
| [induction of tumors of the meningeal membranes in chicks by the avian myeloblastosis virus]. | the meningiomas could be easily produced by intracerebral injection of the avian myeloblastosis virus (0.01 ml of virus suspension with titer 10(9)-10(12) pfu/ml) into highly susceptible (white leghorn anya cross) or randomly bred newborn (24-48 hours) chicken. tumours were observed in 66.7% highly susceptible chickens and in 33.73% of randomly bred chickens. first clinical signs of disease appeared on the 7-9th days after infection. death occurred on the 3-4th days after beginning of the diseas ... | 1988 | 2832019 |
| analysis of hematopoietic and lymphopoietic tissue during a regenerative aplastic crisis induced by avian retrovirus mav-2(o). | infection of 10-day-old chickens with an avian osteopetrosis virus resulted in a severe regenerative aplastic crisis. hematopoietic and lymphopoietic tissues of chickens infected with myeloblastosis-associated virus (of subgroup b, inducing osteopetrosis, mav-2(o] were analyzed for integrated and unintegrated viral dna sequences, cell population shifts, weight changes, and morphological alterations. by 6 days postinfection (p.i.), dna from bone marrow cells and peripheral blood leukocytes (pbl) ... | 1988 | 2833018 |
| structural and functional domains of the myb oncogene: requirements for nuclear transport, myeloid transformation, and colony formation. | the v-myb oncogene of avian myeloblastosis virus causes acute myelomonocytic leukemia in vivo and transforms only myeloid cells in vitro. its product, p48v-myb, is a nuclear protein of unknown function. to determine structure-function relationships for this protein, we constructed a series of deletion mutants of v-myb, expressed them in retroviral vectors, and studied their biochemical and biological properties. we used these mutants to identify two separate domains of p48v-myb which had distinc ... | 1988 | 2835503 |
| immunoblotting with polyclonal and monoclonal antibody to avian myeloblastosis protein p27: studies of liver proteins in chickens with erythroblastosis. | an antigen detected by complement fixation with polyclonal antibody to avian myeloblastosis virus (amv) antigen p27, appears in the livers of chickens inoculated with avian erythroblastosis virus (aev). it can be demonstrated at the 30,000 dalton (30k) molecular weight level by western immunoblotting of electropherograms of aev infected liver extracts. the 30k protein reacted strongly with this polyclonal antibody but only weakly with a monoclonal antibody to the same viral antigen and possible ... | 1987 | 2836998 |
| synthetic peptides as substrates and inhibitors of a retroviral protease. | processing of the gag and pol gene precursor proteins of retroviruses is essential for infectivity and is directed by a viral protease that is itself included in one of these precursors. we demonstrate here that small synthetic peptides can be used as both model substrates and inhibitors to investigate the specificity and molecular parameters of the reaction. the results indicate that a peptide that extends five amino acids but not three amino acids in both directions from a known cleavage site ... | 1988 | 2837759 |
| processed enzymatically active protease (p15gag) of avian retrovirus obtained in an e. coli system expressing a recombinant precursor (pr25lac-delta gag). | processing proteases of avian and mammalian retroviruses cut the polyprotein precursors encoded by the retroviral genes into mature functional proteins. retroviral processing proteases are still a rather poorly characterized group as to their relation to other proteases, specificity, and mechanism of enzymatic action. in avian retroviruses the generation of the processing protease itself comprises a processing cleavage event - the protease p15gag is cut off the carboxy-terminus of a gag polyprot ... | 1988 | 2844587 |
| dna-binding activity associated with the v-myb oncogene product is not sufficient for transformation. | the product of the v-myb oncogene of avian myeloblastosis virus is a nuclear protein with an associated dna-binding activity. we demonstrated that the highly conserved amino-terminal domain of p48v-myb is required for its associated dna-binding activity. this activity is not required for the nuclear localization of p48v-myb. furthermore, the associated dna-binding activity and nuclear localization of p48v-myb together are not sufficient for transformation. | 1988 | 2845150 |
| conserved cysteine and histidine residues of the avian myeloblastosis virus nucleocapsid protein are essential for viral replication but are not "zinc-binding fingers". | the nucleocapsid protein from the rous sarcoma virus has two regions of sequence with the motif cys-xaa-xaa-cys-xaa-xaa-xaa-gly-his-xaa-xaa-xaa-cys. all retrovirus nucleocapsid proteins contain one or two of these motifs, and they represent the only conserved sequences among these proteins. sequence analysis of nucleocapsid from avian myeloblastosis virus shows that it also contains two cys-his sequences and, in fact, differs from the rous sarcoma nucleocapsid protein only in three residues near ... | 1988 | 2845395 |
| retrovirus and periosteal osteogenic cells. morphology study. | a cortical hyperostosis is induced by injection of myeloblastic virus mav 2 (o) in direct contact with periosteum, in chicken just after hatching. it appears an hyperplasia of osteogenic cells of the endoperiosteum. the enchondral ossification develops in normal condition. this lesion is quite different from osteopetrosis. | 1988 | 2849082 |
| avian nephroblastomas induced by a retrovirus (mav-2) lacking oncogene. i. construction of mav-1 and mav-2 proviral restriction maps and preparation of specific proviral molecular subclones. | a 9.8 kb dna fragment containing the complete mav-1 provirus was recloned from the recombinant bacteriophage lambda 311411 (perbal et al., 1985) into the plasmid pat153. a detailed and precise restriction map of the obtained clone (pat-mav-1) was constructed. from compilation of this map and the known sequence of a variable portion of the mav-2 env gene was a restriction map of mav-2 deduced. knowledge of the detailed pat-mav-1 map facilitated the preparation of five specific proviral subclones: ... | 1988 | 2849567 |
| avian nephroblastomas induced by a retrovirus (mav-2) lacking oncogene. ii. search for common sites of proviral integration in tumour dna. | to demonstrate the existence of a common site of integration in independent tumour clones, restriction mapping of the vicinity of integrated mav-2 proviruses in nephroblastoma dna was performed, using southern hybridization with an mav-2 specific probe u3(pat). the results have shown that (1) nephroblastomas are of semiclonal origin. (2) nephroblastoma cells contain an average of 5 clonally located integrated proviruses per diploid genome; they do not contain any detectable amount of non-integra ... | 1988 | 2849568 |
| a point mutation in the dna binding domain of the v-myb oncogene of e26 virus confers temperature sensitivity for transformation of myelomonocytic cells. | we have molecularly cloned a mutant of the v-myb, ets-containing e26 avian leukemia virus which is temperature sensitive for the transformation of myeloid cells. cells infected with this mutant, ts21e26, are immature at 37 degrees c and can be induced to differentiate into resting, macrophage-like cells when shifted to 42 degrees c. the sequence of ts21e26 reveals a single relevant nucleotide alteration resulting in a threonine to arginine change in the highly conserved, putative dna binding v-m ... | 1988 | 2852341 |
| interaction of immunoliposomes with avian myeloblastosis virus and virus transformed cells. | | 1988 | 2855518 |
| a rapid and efficient purification of poly(a)-mrna by oligo(dt)30-latex. | latex particles were covalently linked to the 5'-proximal region of oligo(dt)30. the resultant oligo(dt)30-latex was tested for its hybridizability to poly(a) containing mrna. several advantages were noted as compared to the conventional oligo(dt)30-cellulose column chromatography; (1) a highly efficient (approximately 95%) hybridization occurs in a short reaction period (10min), (2) more than 95% of poly(a) mrna can be recovered from oligo(dt)30-latex by a simple heating followed by brief centr ... | 1988 | 2906428 |
| factor c from rabbit liver. a new poly(dc) and poly[d(g-c)] template-selective stimulatory protein of dna polymerases. | we have undertaken a search for mammalian dna-binding proteins that enhance the activity of dna polymerases in a template sequence-specific fashion. in this paper, we report the extensive purification and characterization of a new dna-binding protein from rabbit liver that selectively stimulates dna polymerases to copy synthetic poly[d(g-c)] and the poly(dc) strand of poly(dc).poly(dg) as well as single-stranded natural dna that contains stretches of oligo(dc). the enhancing protein, a polypepti ... | 1989 | 2925691 |
| determination of the molecular weight of dna-bound protein(s) responsible for gel electrophoretic mobility shift of linear dna fragments examplified with purified viral myb protein. | a protein-dna complex has less gel electrophoretic mobility than the free dna fragment. one parameter for the degree of retardation of a linear dna fragment in a protein-dna complex is the molecular weight of the bound protein(s). the quotient of the migration distances of free dna (m) and protein-dna complex (m') is a function of the molecular weight (mw) of the bound protein(s). based on the evaluation of the lac repressor induced mobility shift of a 203 bp dna fragment containing the lac oper ... | 1988 | 2968540 |
| serum-free conditions for the growth of avian granulocyte and monocyte clones and primary leukemic cells induced by amv, and the apparent conversion of granulocytic progenitors into monocytic cells by a factor in chicken serum. | the supportive activity of chicken serum for the soft-agar growth of chicken granulocyte and monocyte clones could be replaced with defined ingredients [bovine serum albumin (bsa), conalbumin, selenium, linoleic acid and for routine work, a liquid soy lecithin preparation (59% phospholipids, 39% linoleic acid, and less than 2% of inositol and choline)]. the lecithin preparation could be replaced with l-alpha-phosphatidylcholine. the source of colony-stimulating factor was medium conditioned by f ... | 1985 | 2984219 |
| dna-binding activity is associated with purified myb proteins from amv and e26 viruses and is temperature-sensitive for e26 ts mutants. | oncogene protein products from avian myeloblastosis virus, p48v-myb, and from avian leukemia virus e26, p135gag-myb-ets, are located predominantly in the nucleus of nonproducer bone marrow cell clones, as revealed by indirect immunofluorescence. both oncogene proteins were purified by immunoaffinity chromatography using monoclonal antibodies against p19 and immunoglobulins specific for myb, which was expressed in bacteria for antibody production. the purified proteins bind to dna in vitro. in co ... | 1985 | 2985272 |
| cellular transformation by avian viruses. | | 1985 | 2987984 |
| structural characterization of the avian retrovirus reverse transcriptase and endonuclease domains. | the enzymatic domains of the avian retrovirus polymerase (pol) gene have been mapped by the use of peptide antibodies and cooh-terminal amino acid analysis. the processed pol beta polypeptide is cleaved in vivo to yield alpha and pp32. rabbit antibodies were directed against synthetic peptides whose sequence was deduced from the known pol sequence of rous sarcoma virus, prague c (schwartz, d.e., tizard, r., and gilbert, w. (1983) cell 32, 853-869). the rnase h active site of pol was located in t ... | 1985 | 2989284 |
| transformation of brown leghorn chicken embryo fibroblasts by avian myeloblastosis virus proviral dna. | brown leghorn chicken embryo fibroblasts were transfected with a mixture of avian myeloblastosis virus (amv) and myeloblastosis-associated virus type 1 (mav1) proviral dna purified from lambda-charon 4a recombinant clones. a transformed cell line (t1am) able to grow without anchorage in semisolid medium was obtained. the presence of both proviral amv and mav sequences was detected in t1am dna by hybridization with v-myb- and mav1-specific probes. altered amv and mav1 proviral genomes were found ... | 1985 | 2989555 |
| developmental and molecular aspects of nephroblastomas induced by avian myeloblastosis-associated virus 2-o. | avian myeloblastosis-associated virus-induced nephroblastomas are tumors consisting mainly of mesenchymal and epithelial renal elements with variable degrees of differentiation. the spatial distribution of developmental stages reflects a gradient of differentiation from less differential structures in the periphery towards more differentiated structures in the center of the lobules formed in the nephroblastomas. these heterogenic tumors contain discrete virus-cell dna junction fragments and are ... | 1985 | 2989556 |
| retroviral oncogenes and human neoplasia. | | 1985 | 2990442 |
| sites of recombination between the transforming gene of avian myeloblastosis virus and its helper virus. | the sites of recombination between the transforming gene of avian myeloblastosis virus (amv) and its natural helper myeloblastosis-associated virus (mav) have been determined. in amv, the cellular sequence substituting for the viral envelope (env) gene gives rise to a different carboxyl terminus of the dna polymerase. the 5'-recombination site coincides with the rna splice acceptor site for the production of env mrna in mav-infected cells. the 3'-recombination site reveals that the last 11 amino ... | 1985 | 2992154 |
| oncogenes of avian acute leukemia viruses are subsets of normal cellular genes. | | 1985 | 2993114 |
| biologically active proviral clone of myeloblastosis-associated virus type 1: implications for the genesis of avian myeloblastosis virus. | a biologically active myeloblastosis-associated virus (mav) provirus was cloned from a bacteriophage recombinant library constructed from leukemic chicken myeloblast dna. the restriction endonuclease map of this clone was consistent with that of a type 1 mav (mav-1). interference assays of virus recovered from cultured chicken embryo fibroblasts after dna transfection established that the provirus was infectious and confirmed that it belonged to avian retrovirus subgroup a (type 1). antipeptide ... | 1985 | 2993653 |
| structural organization of the mouse proto-myb gene. | proto-myb is a highly conserved cellular gene that is closely related to v-myb, the transforming gene of the avian myeloblastosis virus. we have isolated lambda clones encompassing 21 kbp of mouse dna that contains portions of the proto-myb gene. also, we have isolated a cdna clone containing a 2.5 kbp insert corresponding to mouse proto-myb mrna. by analyzing both the cloned dnas and mouse genome dna, we have constructed a restriction map for mouse proto-myb that extends over 50 kbp. | 1985 | 2998378 |
| poly(dg-dc) in the z-form inhibits e. coli dna polymerase i and amv dna polymerase activity. | the effect of z-conformation of dna on its template activity in dna synthesis reactions in vitro has been studied. normal poly(dg-dc) in the b-form, brominated and unbrominated in the z-form have been compared for their template activity in dna synthesis reactions mediated by amv dna polymerase and e. coli dna polymerase i. the results indicate that poly(dg-dc) in the z-form is totally inactive as a template for dna synthesis and further that it is a strong competitive inhibitor of copying of th ... | 1985 | 2998388 |
| enhanced viral inhibition of lymphocyte mitogenesis in patients with advanced breast cancer. | virus particles are frequently able to non-specifically inhibit the capacity of human peripheral blood lymphocytes to respond to mitogenic or antigenic stimuli. in the case of breast cancer patients with advanced disease, the quantity of virus required to abrogate responsiveness to phytohaemagglutinin (pha) was approximately four-fold less than that found when cells from healthy donors were employed. the results show that such virus co-incubated cultures are deficient with regard to their abilit ... | 1985 | 2998661 |
| nucleotide sequence of cdna clones of the murine myb proto-oncogene. | we have isolated cdna clones of murine c-myb mrna which contain approximately 2.8 kb of the 3.9-kb mrna sequence. nucleotide sequencing has shown that these clones extend both 5' and 3' to sequences homologous to the v-myb oncogenes of avian myeloblastosis virus and avian leukemia virus e26. the sequence contains an open reading frame of 1944 nucleotides, and could encode a protein which is both highly homologous, and of similar size (71 kd), to the chicken c-myb protein. examination of the dedu ... | 1985 | 2998780 |
| detection and localization of the v-myb(amv) gene products of avian myeloblastosis virus by a synthetic peptide antiserum. | an antiserum made against a synthetic peptide from an internal region of the predicted amino acid sequence of the avian myeloblastosis virus (amv) transforming v-myb(amv) gene identified two products, p46v-myb(amv) and p32v-myb(amv), which were localized in the nucleus of amv-transformed myeloblasts. we propose that these proteins are the in vivo products of the v-myb(amv) gene and thus the transforming protein(s) of amv. | 1985 | 2999319 |
| structure and processing of the p2 region of avian sarcoma and leukemia virus gag precursor polyproteins. | we have purified two low-molecular-weight polypeptides from the prague c strain of rous sarcoma virus and have identified these as products of the gag precursor pr76 by protein sequencing and by amino acid analysis. both polypeptides are derived from a stretch of 22 amino acids within pr76 that separates p19 and p10. we refer to this region as p2. together the two cleavage products form the entire p2 region. the junctions of p19 with the amino-terminal fragment of p2 and of p10 with the carboxy- ... | 1986 | 3005658 |
| a cryptic transcription promoter in the myb oncogene of avian myeloblastosis virus. | the potential regulatory signals contained in the v-myb oncogene of avian myeloblastosis virus have been inserted upstream to the herpes simplex type 1 thymidine kinase gene in order to test their promoter activity. the isolation of tk+ transformants after transfection of clone 1d(tk-) mouse cells with the resulting recombinant dnas indicated that the expression of the tk gene was made possible by the myb-derived sequences. analysis of the tk specific rna expressed in different tk+ transformants ... | 1986 | 3006338 |
| nuclease mechanism of the avian retrovirus pp32 endonuclease. | in vivo, the inferred circular retrovirus dna precursor to the provirus contains two long terminal repeats (ltrs) in tandem. we studied the site-specific nicking of supercoiled dna that contains tandem copies of avian retrovirus ltr dna in vitro by using purified avian myeloblastosis virus pp32 endonuclease, mg2+, and viral dna substrates containing different ltr circle junction sequences. the results confirmed our previous observation that the pp32 protein generates two nicks, one in either vir ... | 1986 | 3009900 |
| identification of a provirally activated c-ha-ras oncogene in an avian nephroblastoma via a novel procedure: cdna cloning of a chimaeric viral-host transcript. | retrovirus without oncogenes often exert their neoplastic potential as insertional mutagens of cellular proto-oncogenes. this may be associated with the production of chimaeric viral-host transcripts; in these cases; activated cellular genes can be identified by obtaining cdna clones of bipartite rnas. this approach was used in the analysis of chicken nephroblastomas induced by myeloblastosis-associated virus (mav). one tumor contained a novel mrna species initiated within a mav ltr. cdna clonin ... | 1986 | 3011401 |
| studies of the human c-myb gene and its product in human acute leukemias. | the myb gene is the transforming oncogene of the avian myeloblastosis virus (amv); its normal cellular homolog, c-myb, is conserved across a broad span of evolution. in humans, c-myb is expressed in malignant hematopoietic cell lines and in primary hematopoietic tumors. partial complementary dna clones were generated from blast cells of patients with acute myelogenous leukemia. the sequences of the clones were compared to the c-myb of other species, as well as the v-myb of amv. in addition, the ... | 1986 | 3014652 |
| characterization of monoclonal antibodies to avian leukosis viruses. | hybridoma cell lines secreting monoclonal antibody (mca) to avian leukosis virus (alv) structural proteins p27 and p19 have been established. in an indirect enzyme-linked immunosorbent assay (elisa), mca 6al20 (igg1 isotype) reacted with rpl-40 (alv subgroup a), avian myeloblastosis virus (amv) (a mixture of subgroups a and b), rous-associated virus (rav)-2 (subgroup b), and carr-zilber strain of rous sarcoma virus (cz-rsv) (subgroup d) but not with prague strain of rsv (prc-rsv) (subgroup c) or ... | 1986 | 3015099 |
| expression of molecular clones of v-myb in avian and mammalian cells independently of transformation. | we demonstrated that molecular clones of the v-myb oncogene of avian myeloblastosis virus (amv) can direct the synthesis of p48v-myb both in avian and mammalian cells which are not targets for transformation by amv. to accomplish this, we constructed dominantly selectable avian leukosis virus derivatives which efficiently coexpress the protein products of the tn5 neo gene and the v-myb oncogene. the use of chemically transformed qt6 quail cells for proviral dna transfection or retroviral infecti ... | 1986 | 3016296 |
| structural organization and nucleotide sequence of mouse c-myb oncogene: activation in abpl tumors is due to viral integration in an intron which results in the deletion of the 5' coding sequences. | bacteriophage libraries of mouse dna were screened for sequences homologous to the v-myb oncogene and two overlapping clones containing the v-myb related region were isolated. restriction enzyme mapping, heteroduplex analysis and nucleotide sequence analysis revealed the presence of nine exons. six of these exons are homologous to the v-myb region while the other three exons are derived from the 5' region which is deleted in the viral oncogene. the sequences downstream to the sixth v-myb exon ar ... | 1986 | 3016644 |
| the proto-oncogene c-ets is preferentially expressed in lymphoid cells. | the transforming sequences of the avian acute leukemia virus, e26, contain two distinct oncogenes, v-mybe and v-ets, fused together. by using a probe containing v-ets sequences, polyadenylated transcripts of the c-ets proto-oncogene were detected in avian tissues; they included a major 7.0-kilobase and a minor 2.0-kilobase species. these c-ets mrnas were detected at high levels only in lymphoid organs and in avian t and b lymphoid cell lines. a similar pattern of c-ets transcription was observed ... | 1985 | 3018492 |
| nuclear compartmentalization of the v-myb oncogene product. | nuclei obtained from chicken leukemic myeloblasts transformed by avian myeloblastosis virus were fractionated into various subnuclear compartments, which were then analyzed by specific immunoprecipitation for the presence of the leukemogenic product, p48v-myb, of the viral oncogene. in cells labeled for 30 or 60 min with l-[35s]methionine and in unlabeled exponentially dividing leukemic cells analyzed by western blotting, p48v-myb was detected within the nucleoplasm (29 +/- 9% [standard deviatio ... | 1985 | 3018495 |
| transformation-defective mutant of avian myeloblastosis virus that is temperature sensitive for production of transforming protein p45v-myb. | we have characterized a mutant of avian myeloblastosis virus (strain ga907/7) that shows a reduced capacity to transform myelomonocytic cells at the nonpermissive temperature. myeloblasts transformed by this mutant suffer a substantial decrease in the amount of the transforming protein p45v-myb when shifted from the permissive to the nonpermissive temperature. we presume that the 5- to 10-fold decrease in the amount of p45v-myb causes the loss of the transformed phenotype. the decrease is due to ... | 1985 | 3018515 |
| on the fidelity of dna synthesis. pyrophosphate-induced misincorporation allows detection of two proofreading mechanisms. | the effect of pyrophosphate on the fidelity of in vitro dna synthesis has been examined. pyrophosphate enhances misincorporation by escherichia coli dna polymerase i in copying phi x174 dna. the increased misincorporation is directly proportional to the extent of inhibition of the rate of polymerization. in contrast, pyrophosphate is not detectably mutagenic with avian myeloblastosis virus dna polymerase or dna polymerases alpha and beta from animal cells, which lack associated proofreading acti ... | 1986 | 3020037 |
| covalently linked sequencing primer linkers (splinkers) for sequence analysis of restriction fragments. | a new method for direct sequence analysis of dna restriction fragments uses synthetic covalently linked complementary oligodeoxynucleotides, as universal sequencing primer linkers (splinkers). these splinkers were designed to contain an inverted repeat sequence which forms a double-stranded hairpin structure with a known restriction site. the splinkers were characterized by their ability to be self-ligating (dimerized) and by their restriction digest product analysis of both the monomer and dime ... | 1986 | 3023188 |
| subnuclear localization of proteins encoded by the oncogene v-myb and its cellular homolog c-myb. | the retroviral transforming gene v-myb encodes a 45,000-mr nuclear transforming protein (p45v-myb). p45v-myb is a truncated and mutated version of a 75,000-mr protein encoded by the chicken c-myb gene (p75c-myb). like its viral counterpart, p75c-myb is located in the cell nucleus. as a first step in identifying nuclear targets involved in cellular transformation by v-myb and in c-myb function, we determined the subnuclear locations of p45v-myb and p75c-myb. approximately 80 to 90% of the total p ... | 1986 | 3023837 |
| coordinate regulation of myelomonocytic phenotype by v-myb and v-myc. | both avian myeloblastosis virus (by the action of v-myb) and avian myelocytomatosis virus mc29 (by the action of v-myc) transform cells of the myelomonocytic lineage. whereas avian myeloblastosis virus elicits a relatively immature phenotype, cells transformed by mc29 resemble mature macrophages. when cells previously transformed by v-myb were superinfected with mc29, their phenotype was rapidly altered to that of a more mature cell. these superinfected cells expressed both v-myb (at a level sim ... | 1986 | 3023905 |
| human monoclonal antibodies derived from lymph nodes of a patient with breast carcinoma react with mumtv polypeptides. | nine human hybridoma cell lines were established from a fusion of axillary lymph node lymphocytes of a patient with breast ductal carcinoma with a human lymphoblastoid cell line. the human hybridoma nature of the fusion products was confirmed by chromosomal analysis and hla typing. the hybridomas are stable over a year of growth, and can be frozen, thawed and regrown. the carcinoma cells of the patient harbor mouse mammary tumor virus (mumtv) cross-reacting antigens. the patient's serum and the ... | 1987 | 3024800 |
| structure of the protein encoded by the chicken proto-oncogene c-myb. | the retroviral oncogene v-myb arose by transduction of the chicken proto-oncogene c-myb. we isolated and sequenced cdna that represents the entire coding domain of chicken c-myb. by transcribing the cdna into mrna in vitro and then translating the rna, we were able to document the integrity of the cdna and to identify the codon responsible for initiation of translation from c-myb. two different alleles of v-myb are extant, one in the genome of avian myeloblastosis virus (amv) and the other in th ... | 1986 | 3025608 |
| env-encoded residues are not required for transformation by p48v-myb. | the v-myb oncogene of avian myeloblastosis virus induces acute myeloblastic leukemia in chickens and transforms avian myeloid cells in vitro. the protein product of this oncogene, p48v-myb, is partially encoded by the retroviral gag and env genes. we demonstrated that the env-encoded carboxyl terminus of p48v-myb is not required for transformation. our results showed, in addition, that a coding region of c-myb which is not essential for transformation was transduced by avian myeloblastosis virus ... | 1987 | 3027417 |
| characterization of a virus-specific proteolytic activity processing the gag precursor of the simian sarcoma-associated virus. | the proteolytic processing of the gag precursor polypeptide pr65gag of simian sarcoma-associated virus (ssav) has been studied in vivo and in vitro. in ssav-infected cells (i.e., in vivo) proteins of 52 and 38 kda and the viral protein p30 could be immunoprecipitated with anti-p30 serum. this cleavage pattern is only in part imitated by in vitro cleavage of the isolated pr65gag with avian myeloblastosis virus (amv) protease p15. however, in vitro incubation of isolated pr65gag with detergent-dis ... | 1987 | 3027976 |
| myb-specific probes. | | 1987 | 3029704 |
| expression of a truncated v-myb product in transformed chicken embryo fibroblasts. | transformed cells have been isolated after transfection of chicken embryo fibroblasts (cef) with the dna of a recombinant clone (kxa 3457) in which the v-myb sequences are flanked by the two amv-ltrs. abnormal myb-specific rna species and myb-related polypeptides were found to be expressed in these cells, suggesting that transformation of cef by v-myb might require alterations of the oncogene product. | 1987 | 3032682 |
| two exons specific for the myb proto-oncogene found upstream from the avian myeloblastosis virus-transduced myb sequences. | a partial restriction map of cloned 5.42-kb chicken dna (clone p542, perbal et al. 1983), covering a portion of the c-myb locus, is presented. the 5' end of the v-myb gene (approximately 0.5 kb) is located at the 3' end of p542 dna, the remainder are the cellular sequences not transduced by avian myeloblastosis virus. two non-contiguous dna segments were detected within these cellular sequences which code for the 5' end of c-myb mrna. these two exons, designated e1 and e2, are separated by a app ... | 1987 | 3032698 |
| v-myb does not prevent the expression of c-myb in avian erythroblasts. | the v-myb oncogene of avian myeloblastosis virus transforms myeloid cells exclusively, both in vivo and in vitro. the c-myb proto-oncogene from which v-myb arose is expressed at relatively high levels in immature hematopoietic cells of the lymphoid, erythroid, and myeloid lineages but not in myeloblasts transformed by v-myb. this finding suggested that the nuclear v-myb gene product p48v-myb might act directly to inhibit the normal expression of the c-myb gene. i have therefore used a selectable ... | 1987 | 3041047 |
| structure, expression and alternative splicing of the human c-ets-1 proto-oncogene. | the avian acute leukemia virus e26 induces a mixed erythroid-myeloid leukemia in chicken and carries two distinct oncogenes, v-myb and v-ets. it was shown that there exist two ets loci in humans, c-ets-1 and c-ets-2, which were mapped to human chromosome 11 and 21, respectively. the human c-ets-1 proto-oncogene locus was shown to translocate from chromosome 11 to 9 or 4 in certain patients with acute monocytic leukemia. in order to understand the functional role of c-ets-1 proto-oncogene, we hav ... | 1988 | 3060801 |
| dihydrothymidine and thymidine glycol triphosphates as substrates for dna polymerases: differential recognition of thymine c5-c6 bond saturation and sequence specificity of incorporation. | the ability of dihydrothymidine (dhdttp) and thymidine glycol (dttp-gly) 5'-triphosphates to serve as substrates for different dna polymerases was investigated. dhdttp but not dttp-gly was used as a substrate by e. coli dna polymerase i (pol i). within the detection limit of the assay used, neither t4 dna polymerase nor avian myeloblastosis virus (amv) reverse transcriptase used dhdttp or dttp-gly as substrates. the ability of dhdttp and dttp-gly to undergo enzyme-catalyzed turnover to the monop ... | 1988 | 3060857 |
| antibodies to the evolutionarily conserved amino-terminal region of the v-myb-encoded protein detect the c-myb protein in widely divergent metazoan species. | antibodies directed against a bacterial fusion protein that contains the domain encoded by the highly evolutionarily conserved 5' one-third of the v-myb oncogene of avian myeloblastosis virus (amv) detect the protein products of various members of the myb gene family. immunoprecipitation or immunoblot analyses with these antibodies yielded the following information. first, the products of the v-myb oncogenes of amv (p48v-myb) and of e26 virus (p135gag-myb-ets) contain this highly conserved amino ... | 1986 | 3088565 |
| effects of altered nucleotide concentrations on the fidelity of dna replication. | | 1985 | 3158301 |
| inhibition (in vitro) of replication and of the cytopathic effect of human immunodeficiency virus by an extract of the culture medium of lentinus edodes mycelia. | an extract of culture medium of lentinus edodes mycelia (lem) was prepared. this was further fractionated by 50% ethanol precipitation and both the resulting product, e-p-lem, and lem were studied to evaluate their effect on the activity of human immunodeficiency virus (hiv) in vitro. the experiments were performed using either a cell-free infection system with mt-4 cells, or a cell-to-cell infection system with molt-4 cells, which induces multinucleated giant cells very efficiently. e-p-lem alm ... | 1988 | 3173237 |
| a centrifugation method for separation of plant viral genomic and subgenomic rnas. | using alfalfa mosaic virus (amv) as a model, a simple method for separating plant viral genomic rnas from their subgenomic counterparts was established. the method relies on sucrose gradient fractionation under carefully selected conditions of centrifugation and fraction collection. the rna components are recovered in nearly quantitative yield and have full biological activity as measured by infectivity of the reconstituted rnas in suitable protoplasts and plant hosts. the individual rnas, on th ... | 1988 | 3181424 |
| reduced in vivo nonspecific resistance to listeria monocytogenes infection during avian retrovirus-induced immunosuppression. | ten-day-old chickens infected with an avian osteopetrosis virus [mav-2(o)] were more susceptible to challenge with listeria monocytogenes than virus-free chickens, as demonstrated by reduced bacterial clearance from their spleens. reduced clearance of l. monocytogenes was observed throughout a 26-day period after mav-2(o) infection. | 1988 | 3202762 |
| sequential study of visceral lesions caused by isolates of an avian osteopetrosis virus (myeloblastosis-associated virus). | ten-day-old chicken embryos were inoculated with isolates of myeloblastosis-associated virus that induced osteopetrosis of slow or rapid onset. bursa of fabricius, thymus, spleen, bone marrow, kidney, liver, and lung were examined at 15, 17, and 19 days in ovo and at 7 and 25 days after hatching by histologic and immunoperoxidase techniques. tissues from 19-day-old in ovo embryos also were examined by electron microscopy. the lymphoid organs of embryos inoculated with all isolates manifested cha ... | 1988 | 3223670 |
| proto-oncogene expression in chicken leukemic cells induced by avian myeloblastosis virus. | sixteen proto-oncogenes which have generated retroviral oncogenes were tested for their expression in chicken leukemic cells induced by avian myeloblastosis virus (amv) and five were found to be expressed (c-ets, c-fps, c-mht, c-myc, and c-rel). the size of the c-fps transcript (4.0 kb) was not in good agreement with the size (approximately 3.0 kb) previously reported but was uniform in the leukemic cells from 10 different chickens. the size of the other proto-oncogene transcripts appeared norma ... | 1988 | 3226723 |
| solid-phase synthesis of a nonadecapeptide coded for by the v-myb oncogene. | a nonadecapeptide comprising a predicted b-cell determinant from the v-myb oncoprotein was synthesized by merrifield's solid-phase method. hydrogen chloride in dichloromethane was used for protective t-butyloxycarbonyl group removal; the deprotection was monitored using a new qualitative deprotection test. the nonadecapeptide coupled to a carrier elicited a high titre of protein-reactive antipeptide antibodies. | 1988 | 3259212 |
| characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc. | we have characterized a simple method that uses 65zncl2 to detect zinc-binding proteins that have been immobilized on nitrocellulose. conditions have been identified that permit the detection of as little as 1 microgram of some zinc-binding proteins. the specificity of the binding is indicated by the ability of other divalent metal ions to compete with 65zn(ii) in this assay. we have used this technique to provide evidence that the nucleic acid-binding gag protein of retroviruses also binds zinc ... | 1988 | 3260031 |
| evidence that the 5'-untranslated leader of mrna affects the requirement for wheat germ initiation factors 4a, 4f, and 4g. | the efficiency of translation of alfalfa mosaic virus (amv) rna 4, barley alpha-amylase (b alpha a) mrna, and two chimeric mrnas, amv 4-b alpha a and b alpha a-amv 4 (in which the 5' leader sequences of the two mrnas were interchanged), was measured in an s30 extract from wheat germ and a fractionated system from wheat germ in which translation could be made dependent upon initiation factor (eif) 3, 4a, 4f, or 4g. in the s30 system, amv rna 4 and the chimeric mrna amv 4-b alpha a are translated ... | 1988 | 3260235 |
| fluorescence studies on the coat protein of alfalfa mosaic virus. | the intrinsic luminescence of different forms of the alfalfa mosaic virus (amv) strain 425 coat protein has been studied, both statically and time resolved. it was found that the emission of the protein (mr 24,250), which contains two tryptophans at positions 54 and 190 and four tyrosines, is completely dominated by tryptophan fluorescence. the high fluorescence quantum yield indicates that both tryptophans are emitting. surprisingly, the fluorescence decay is found to be strictly exponential, w ... | 1986 | 3271050 |
| some aspects of dna polymerase functioning. | the group of dna polymerases was studied using some new nucleoside 5'-triphosphate analogs with termination substrate properties. among dna polymerases tested the least specific appeared reverse transcriptases of retroviruses and the most specific were dna polymerases alpha type from high eucaryotes including mammalians. | 1987 | 3320974 |
| mechanism of activation of the myb oncogene in myeloid leukemias. | | 1987 | 3326465 |
| the myb oncogene. | the highly conserved, single copy c-myb gene has been independently transduced by two avian acute leukemia viruses, amv and e26. this gene has also undergone insertional mutagenesis by non-acutely transforming murine leukemia viruses in a number of hematopoietic tumors. the common denominator of these retroviral activations of c-myb appears to be truncation of the normal coding region at either or both ends. the role of point mutations in myb-induced leukemogenesis is currently unknown. the prod ... | 1986 | 3333362 |
| structure and mechanism of activation of the myb oncogene. | the results summarized in this review show that the normal chicken myb gene codes for a protein of 77 kd which appears to play an important role in the control and/or differentiation of hematopoietic cells of myeloid and t lymphoid series. the activation of this gene has been observed in chicken and murine systems. in the avian system, this has been achieved by transduction of the myb oncogene into a retrovirus. such a transduction resulted in the deletion of coding sequences from both the 5' an ... | 1986 | 3333363 |
| pharmacokinetics and chemotherapeutic efficacy of adriamycin encapsulated in immunoliposomes against avian myeloblastosis virus infection. | immunoliposomes were prepared using rabbit anti-amv gp80 igg for the targeted chemotherapy of avian myeloblastosis virus infection. adriamycin was encapsulated into immunoliposomes and used for in vivo studies. comparative pharmacokinetics of free drug, drug encapsulated in free liposomes and of drug encapsulated in immunoliposomes in the virus-infected cells revealed that (i) the drug encapsulated in liposomes was cleared from the plasma slowly, and (ii) the drug encapsulated in immunoliposomes ... | 1988 | 3366852 |
| further implications for the evolutionary relationships between tripartite plant viruses based on cucumber mosaic virus rna 3. | the nucleotide sequence of the rna 3 of the q-strain of cucumber mosaic virus (q-cmv) has been reinvestigated and supporting partial amino acid sequence data obtained for the coat protein. corrections to the previously published sequence of rna 3 [a. r. gould and r. h. symons (1982) eur. j. biochem. 126, 217-226] result in changes to the size and composition of the putative 3a and coat proteins. analysis of the nucleotide sequence revealed a 14-nucleotide sequence present in the intercistronic r ... | 1988 | 3388769 |
| differentiation of an epiperiosteal sheath in avian hyperostosis induced by myeloblastosis associated virus mav-2 (0). | if myeloblastosis associated virus mav-2(0) is injected in chick embryos, it causes a severe, irreversible and generalized hyperostosis which subsequently follows a chronic course. an epiperiosteal sheath forms at the site of the lesion, whose development follows that of the hyperostosis. the sheath contains not only cell infiltrates, blood vessels and myoblasts, but also highly specialized cells such as pacinian corpuscles. the structural lesions are absolutely different from these described in ... | 1988 | 3400973 |
| new antibiotics sf2315a and b produced by an excellospora sp. i. taxonomy of the strain, isolation and characterization of antibiotics. | two new antibiotics sf2315a and b have been isolated from culture filtrate of an actinomycete strain, excellospora viridilutea sf2315. they are weakly active against gram-positive bacteria and inhibited reverse transcriptase of avian myeloblastosis virus. empirical molecular formula of antibiotics sf2315a and b were determined to be c19h16o5 and c19h20o6, respectively. | 1988 | 3417555 |
| [a new terminator of dna biosynthesis--possible conformation analog of the substrate in a dna-synthesizing complex]. | 2',3'-dideoxy-2',3'-dehydrothymidine 5'-triphosphate (dddttp) reveals the termination substrate properties in the dna synthesis catalyzed by e. coli polymerase i (klenow fragment), rat liver dna polymerase beta, calf thymus terminal deoxynucleotidyl transferase, and reverse transcriptase of avian myeloblastosis virus but does not affect calf thymus dna polymerase alpha. for dna polymerase i, dddttp by an order of magnitude is more effective than any known termination substrate. it is supposed th ... | 1987 | 3435558 |
| a sequential study of bone lesions caused by isolates of an avian osteopetrosis virus, mav-2(0). | the pathogenesis of avian osteopetrosis caused by rapid and slow-onset isolates of myeloblastosis associated virus, mav-2(0), was studied by inoculation of 10-day-old chick embryos with virus. femur and calvarium were examined at 15, 17 and 19 days in ovo and 7 and 25 days after hatching by histologic and immunoperoxidase techniques. femur and calvarium were also examined by electron microscopy at 17 and 19 days in ovo and at 7 days after hatching. avian osteopetrotic bone lesions were character ... | 1987 | 3446259 |
| enhanced translation of chimaeric messenger rnas containing a plant viral untranslated leader sequence. | eukaryotic messenger rnas are translated with unequal efficiencies in vivo and in vitro and the molecular basis of this phenomenon is not understood. as an approach to understanding the role of the 5' untranslated leader sequence in regulating mrna translational efficiency, chimaeric mrnas have been generated by joining a heterologous leader to complementary dna (cdna) sequences, followed by in vitro transcription using sp6 rna polymerase and in vitro protein synthesis. we used the untranslated ... | 1987 | 3492677 |
| identification of c-myb (chicken), c-myb (mouse) and v-myb (amv) protein products by immunoprecipitation with antibodies directed against a synthetic peptide. | a synthetic nonadecapeptide (il 19) derived from a sequence of v-myb was covalently bound to haemocyanin and used for immunization. anti-il 19 serum immunoprecipitated a 75 kda protein in the lysate of metabolically labelled chicken and murine thymus cells. presaturation of the serum with il 19 abolished this immunoprecipitation, thus indicating that the product of c-myb in both chicken and murine thymuses is the 75 kda protein (p75c-myb). anti il 19 serum also precipitated p48v-myb in the lysat ... | 1986 | 3527747 |
| identification and characterization of the protein encoded by the human c-myb proto-oncogene. | we have identified the product of the human c-myb proto-oncogene as a 80,000-mr protein, p80c-myb, by using polyclonal and monoclonal antibodies raised against a bacterially synthesized polypeptide from the amino terminus of the viral myb protein. p80c-myb shares at least two distinct antigenic sites with the amino terminal region of the v-myb protein. p80c-myb is found only in hematopoietic cells or in cells that contain amplified c-myb genes. like the chicken myb proteins, p80c-myb is a nuclea ... | 1986 | 3530745 |
| insulin-like growth factor i induces c-fos messenger ribonucleic acid in l6 rat skeletal muscle cells. | insulin-like growth factor i (igf-i) mediates growth promotion of gh on specific target cells. as other growth factor action has been associated with oncogene induction, we examined whether igf-i activated cellular oncogenes in l6 rat muscle cells, known target cells of igf-i stimulatory action. quiescent cells were incubated in serum-free defined medium with igf-i (amgen recombinant analog, thr59) from 5 min to 4 h. 32p-labeled dna was first prepared from igf-i-treated cell poly(a) rna using av ... | 1987 | 3536454 |
| identification in chicken macrophages of a set of proteins related to, but distinct from, the chicken cellular c-ets-encoded protein p54c-ets. | using an antiserum to a bacterially expressed polypeptide corresponding to 56 amino acids of v-ets, we previously identified in chicken tissues a protein of 54 kd (p54c-ets) which shares extensive sequence homology to the v-ets-encoded domain of the e26-transforming protein p135gag-myb-ets and is thus apparently encoded by the c-ets proto-oncogene. we report here that the anti-ets serum specifically identifies in chicken cells a second set of proteins of 60 kd (p60), 62 kd (p62) and 64 kd (p64) ... | 1986 | 3536486 |
| protein product of proto-oncogene c-mil. | using antipeptide antibodies with specificity for the carboxyl termini of v-raf and v-mil protein products, two proteins with apparent molecular weights of approximately 71,000/73,000 and 215,000 were detected in immunoprecipitates from normal uninfected chicken cells. the 71,000/73,000-molecular-weight protein was identified as the product of the c-mil proto-oncogene by the close structural relationship of its 42,000-molecular-weight carboxyl-terminal domain to the v-mil-encoded domain of the h ... | 1986 | 3537695 |
| template-selective stimulation of diverse dna polymerases by the murine dna-binding protein factor d. | factor d, a template-selective dna polymerase-alpha stimulatory protein from mouse liver (fry, m., lapidot, j., and weisman-shomer, p. (1985) biochemistry 24, 7549-7556) is shown here to enhance the activities of diverse dna polymerases with a cognate template specificity. dna synthesis catalyzed by escherichia coli dna polymerase i, avian myeloblastosis virus polymerase, and some mammalian alpha- and gamma-polymerases was increased by factor d. with every enhanced polymerase, factor d increased ... | 1987 | 3597397 |
| [tumors of the brain vascular plexuses caused by the avian myeloblastosis virus]. | a series of experiments on the induction of tumours in the central nervous system with the help of avian myeloblastosis virus was carried out. 142 highly sensitive and inbred chickens, 1 or 2 days of age, were used. the intracerebral virus dose was 0.01-0.02 ml. the titer was 10(9)--10(12) pfu/ml. 25 tumours of choroid plexus were induced during the experiments, 3 tumours developed in the control after intravenous inoculation. out of 33 chickens highly sensitive to amv tumours of choroid plexus ... | 1987 | 3620686 |
| subnuclear associations of the v-myb oncogene product and actin are dependent on ionic strength during nuclear isolation. | the method used to isolate nuclei has a direct effect on the subnuclear association of the v-myb product, p48v-myb, and nuclear actin. analysis of nuclei subjected to various isolation procedures showed that disruption of native nuclear structure during hypotonic treatment resulted in dissociation of p48v-myb from the nuclear matrix. | 1987 | 3670313 |
| sequence-specific dna-binding proteins which interact with (g + c)-rich sequences flanking the chicken c-myc gene. | the interaction of nuclear sequence-specific dna-binding proteins from definitive chicken erythrocytes, thymus and proliferating transformed erythroid precursor (hd3) cells with the 700-base-pair (700-bp) dna 5'-flanking region of the chicken c-myc gene was investigated by in vitro footprint analysis. the major hd3 protein-binding activity binds to a site (site v) 200 bp upstream from the 'cap' site but, after further fractionation, a second distinct binding activity is detected to a site (site ... | 1986 | 3743569 |
| nucleotide sequence of chicken c-myb complementary dna and implications for myb oncogene activation. | avian myeloblastosis virus (amv), like other acute transforming viruses, arose by recombination between its helper virus and host cellular sequences. the latter sequences, termed v-myb, are responsible for the oncogenic properties of the virus. amv causes acute myeloblastic leukaemia in chickens and transforms a specific class of haematopoietic cells in vitro, but does not induce morphological transformation of cultured fibroblasts, suggesting that only a restricted target-cell population is res ... | 1986 | 3753748 |
| avian monocytic leukemia cells release fibroblast growth factor: implications to associated myelofibrosis. | the marrow of chicks with leukemia induced by avian 'myeloblastosis' virus (amv) exhibited a 5-10-fold increase in the number of fibroblast colony-forming cells (cfu-f). the increased cfu-f correlated with a mild fibrosis which can be seen in the marrow of these animals. fibroblast proliferation likely was not simply due to the presence of leukemic cells because addition of formaldehyde-fixed peripheral leukemic cells failed to initiate cfu-f growth. conditioned medium (cm) from day-4 cultures o ... | 1985 | 3866125 |
| molecular basis of human b cell neoplasia. | | 1985 | 3880642 |
| [involvement of bone tissue in chicken infected with the myeloblastic mav 2-0 virus. generalized cortical hyperostosis]. | an affection of osseous tissue appears in chicken have been injected at the embryonic state with retrovirus myeloblastic mav 2-0 in solution. the structural lesions are different from these examined in human or murine osteopetrosis, considered to be an general cortical hyperostosis. | 1985 | 3919885 |
| induction of colony-stimulating factor from quiescent fibroblasts by avian macrophages and monocytic leukemic cells. | confluent, quiescent chick embryo fibroblasts maintained in protein-free medium released csf(s) active on granulocyte and monocyte progenitors and on monocytic leukemia cells induced by avian 'myeloblastosis' virus (amv) when exposed to formaldehyde-fixed amv leukemic cells. the csf was assayed under serum-free conditions. of several normal cell types tested including populations enriched for blast cells, only macrophages exhibited this capacity. | 1985 | 3935882 |
| influence of template primary and secondary structure on the rate and fidelity of dna synthesis. | high resolution gel electrophoresis was used to monitor the successive addition of dnmp residues onto the 3'-oh ends of discrete 5'-32p-primers, during dna synthesis on natural templates. resulting autoradiographic banding patterns revealed considerable variation in the relative rates of incorporation at different positions along the template. the pattern of "pause sites" along the template was unique for each of three different dna polymerases (polymerase i (the "large fragment" form of escheri ... | 1985 | 3972819 |
| antiviral activity of liposome-encapsulated cupric complex of isonicotinic acid hydrazide against avian myeloblastosis virus infection. | | 1985 | 4077135 |
| [the periosteum in experimental avian hyperostosis induced by mav 2-0 myeloblastosis virus. differential diagnosis from osteopetrosis]. | diaphyseal tibial bones of 11-14-17 th day-old hatched chicks infected with retrovirus myeloblastic mav 2-0 were examined by conventional optical microscopy. the characteristic lesion is osteoblastic hyperplasia with the development of spongious bone. the new formed bone contains abnormal chondroid tissue. the relation between osseous and cartilaginous tissues in this experimental model is discussed. as connective tissue took part in the constitution of this hyperostosis, this osteopathy may be ... | 1985 | 4091574 |