Publications

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morphological conversion of cell cultures by avian myeloblastosis virus. 196113864532
biologic response of nephrogenic cells to avian myeloblastosis virus. 196214026087
properties of cells infected with avian myeloblastosis virus. 196214010247
studies on the assay and multiplication of avian myeloblastosis virus. 196313997685
[increase in production of metastases by c-13-labeled bhk21 hamster cells after infection in vitro by the avian myeloblastosis virus]. 196414196162
the nucleotide composition of the rna of the avian myeloblastosis virus (bai, strain a) and of the nucleic acids of leukaemic myeloblasts. (a comparative study). 196414271457
studies on the bai strain a (avian myeloblastosis) virus. i. production and examination of potent virus-specific complement-fixing antisera. 196414227044
studies on the bai strain a (avian myeloblastosis) virus. ii. some properties of viral split products. 196414227045
cytopathogenicity of fowl tumour viruses (rsv and amv) in normal and transformed cells of human origin. 196414189810
[research on the biosynthesis of the ribonucleic acid of the avian myeloblastosis virus]. 19654284721
[demonstration of a nuclease activity associated with the avian myeloblastosis virus during attempted purification of this virus and its ribonucleic acid]. 19654284727
the nucleic acid from avian myeloblastosis virus compared with the rna from the bryan strain of rous sarcoma virus. 19654286563
[demonstration of a ribonucleic acid of very high molecular weight in the avian myeloblastosis virus]. 19654284934
[inhibition of the multiplication of the myeloblastosis virus (amv) in vitro by guanidine]. 19654285130
effect of avian myeloblastosis virus in the japanese quail.moscovici, carlo (university of colorado medical center, denver), and e. h. macintyre. effect of avian myeloblastosis virus in the japanese quail. j. bacteriol. 92:1141-1149. 1966.-avian myeloblastosis virus (amv) induced a spectrum of neoplasms in japanese quail (coturnix coturnix japonica) which was similar to that observed in the chicken, with one exception: the total absence of acute myeloblastic leukemia in quail. studies in vivo as well as in vitro suggested that the cause for this differe ...19664288797
[homology between the genome of avian myeloblastosis virus (amv) and the cellular genome]. 19664291018
high molecular weight rna from avian myeloblastosis virus. 19664287851
avian myeloblastosis virus (amv) biosynthesis in chick embryo cells: studies of the particles released by trypsin from the infected cells. 19664287875
actinomycin d inhibition of avian myeloblastosis virus production by chick-embryo fibroblasts. 19664288052
in vivo replacement of the helper virus in bryan's strain of rous sarcoma virus by amv and rpl12 viruses. 19664288867
[electron microscope examination of rna from avian myeloblastosis virus]. 19664288988
a quantitative assay for avian myeloblastosis virus. 19674292479
zone electrophoresis of the proteins of avian myeloblastosis virus. 19674292644
induction of "avian leucosis group-specific" antigen synthesis in chick embryo fibroblasts by template active rna from avian myeloblastosis virus. 19674295012
transient inhibition of avian myeloblastosis virus reproduction by amethopterin and fluorodeoxyuridine.avian myeloblastosis virus cannot initiate its reproduction in the presence of amethopterin or fluorodeoxyuridine. this inhibition is reversed by thymidine. addition of either inhibitor after virus production has started does not inhibit further virus synthesis. in presence of either inhibitor, deoxyribonucleic acid synthesis is inhibited by over 90%, but ribonucleic acid synthesis is not affected. cells resume their normal growth rate 24 hr after removal of either inhibitor.19674316249
the role of the bursa-dependent lymphoid tissue in oncogenesis by avian myeloblastosis virus. 19674291304
hybridization of avian myeloblastosis virus rna with dna from chick embryo cells. 196718614099
characterization of avian leucosis group-specific antigen from avian myeloblastosis virus. 19684295292
[synthesis of uridilic nucleotides and tmp in bone marrow of chickens infected with avian myeloblastosis virus]. 19684298767
effects of genetic cellular resistance on cell transformation and virus replication in chicken hematopoietic cell cultures infected with avian myeloblastosis virus (bai-a). 19684300361
the n-terminal amino acid of an avian leukosis group-specific antigen from avian myeloblastosis virus. 19694306524
studies on the rna from avian myeloblastosis virus. 19694302800
the oncogenic effects of nontransforming viruses from avian myeloblastosis virus. 19694308011
incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions.freshly explanted leukemic myeloblasts produce avian myeloblastosis virus (amv) at a constant rate without any obvious cytopathic effect; therefore, subviral components are continually synthesized at a steady rate. the incorporation of various radioactive precursors into virions was monitored by determination of radioactivity in purified virus after density equilibrium sedimentation in preformed sucrose gradients. the kinetics of incorporation of (3)h-uridine have shown that there is an average ...19694311791
an improved adenosine triphosphatase assay for physical particles of avian myeloblastosis virus (amv). 19694311838
[demonstration of virus particles of amv type in a transplantable hamster tumor: hp]. 19694318901
[heterotransmission of avian myeloblastoma virus (amv) to the hamster]. 19694306857
transformation of chick fibroblast cultures with avian myeloblastosis virus. 19694307102
deoxyribonucleic acid polymerase associated with rous sarcoma virus and avian myeloblastosis virus: properties of the enzyme and its product.deoxyribonucleic acid (dna) polymerase activity can be elicited in purified preparations of avian myeloblastosis virus and rous sarcoma virus (schmidt-ruppin strain) by treatment with nonionic detergent. the enzyme(s) and its synthetic products appear to be virion-associated. enzymatic activity can be inhibited by pretreatment with either ribonuclease (8- to 10-fold inhibition) or actinomycin d (twofold inhibition). by contrast, rifampin has little, if any effect. the enzyme(s) synthesizes two p ...19704320696
isolation of a second avian leukosis group-specific antigen (gs-b) from avian myeloblastosis virus.gs-b, a second group-specific antigenic protein of the avian leukosis group, has been isolated from tween 80-ether treated avian myeloblastosis virus by sephadex and carboxymethylcellulose chromatography, and compared by immunological and chemical means with the antigen (gs-a) described previously.19704331722
studies on single foci of hematopoietic cells transformed by avian myeloblastosis virus. 19704318985
infection of chick embryo fibroblasts with template active rna from avian myeloblastosis virus. 19704313399
dna complementary to viral rna in leukemic cells induced by avian myeloblastosis virus.nucleic acid hybridization studies were made between 71s-amv-rna and dna from leukemic myeloblasts and from normal chicken cells. there was homology between the viral rna and chicken cell dna and to a greater extent between viral rna and leukemic cell dna. leukemic cell dna hybridized approximately twice as much viral rna as did normal chicken dna. thermal melting studies showed that the viral rna bound to normal and leukemic cell dna consists of long polynucleotides (t(m) = 87 degrees and 92 de ...19704317913
coiled structure of the nucleocapsid of avian myeloblastosis virus. 19704320687
isolation of amino acid acceptor rna from purified avian myeloblastosis virus. 19704320940
mechanism of carcinogenesis by rna tumor viruses. 3. formation of rna, dna complex and duplex dna molecules by the dna polymerase (s) of avian myeloblastosis virus.dna polymerase activity can be unmasked in avian myeloblastosis virus (amv) by treatment with the nonionic detergent nonidet p-40. two products are formed: (1) rna.dna hybrid molecules and (2) duplex dna molecules. the kinetics of dttp incorporation into dna are biphasic: an initial rapid reaction for 4 min at 37 degrees c with a minimal polymerization rate of 10-20 nucleotides per see, and a second reaction at about half the initial rate. viral rna.dna complexes are detected as early as 30 sec ...19704322024
minor rna and other components of host origin intrinsic to avian leukosis virus particles. 19704376356
virological analysis of individual foci induced in hematopoietic cells by avian myeloblastosis in virus. 19704376358
haemagglutination reaction with avian myeloblastosis virus. 19714397346
nucleoside diphosphokinase activity associated with dna polymerases.nucleoside diphosphokinase activity is present in highly purified preparations of dna polymerase from micrococcus luteus and escherichia coli, and in a partially purified dna polymerase from avian myeloblastosis virus. the activity is also observed in the protein fragment of molecular weight 76,000 that is produced by subtilisin cleavage of dna polymerase i from e. coli. the ndp kinase activity in dna polymerase preparations from m. luteus uses various ribo- and deoxyribonucleoside di- and triph ...19714332252
primer requirement and template specificity of the dna polymerase of rna tumor viruses.polyribonucleotides will act as efficient templates for the dna polymerases found in the virions of avian myeloblastosis virus and mouse leukemia virus if a short complementary oligodeoxyribonucleotide primer is added. synthesis of the complementary polydeoxyribonucleotide continues until an amount of polymer equal to the amount of initial template has been produced. the two viruses show slightly different specificities toward the four homoribopolymers. polydeoxyribonucleotides are generally muc ...19714327004
[avian myeloblastosis virus. model for study of leukemia due to virus]. 19714335902
association of 4s ribonucleic acid with oncornavirus ribonucleic acids.oncornavirus 60 to 70s ribonucleic acids (rna), such as those from avian myeloblastosis virus, schmidt-ruppin virus, or mouse sarcoma-mouse leukemia viruses, isolated by conventional techniques, contain 4s transferlike rna molecules that are released upon dissociation of the 60 to 70s rna with heat. the 4s rna represents 2.5 to 3.0% of the rna in the 65s aggregate or 4 to 5 molecules per molecule of 35s rna formed.19714329970
specific rna methylase associated with avian myeloblastosis virus. 19714331547
base composition differences between avian myeloblastosis virus transfer rna and transfer rna isolated from host cells.using a novel chemical tritium derivative method, we have determined the base composition of 4s rna isolated from an rna tumor virus, the avian myeloblastosis virus, and from normal and neoplastic host cells. extensive differences were detected, particularly with respect to the amount of methylated bases in the viral rna. the viral 4s rna, which fulfills the criteria for designation as transfer rna, appears to be derived from a precursor pool that is different from the precursor population of ho ...19714332019
ultrastructural comparison of a virus from a rhesus-monkey mammary carcinoma with four oncogenic rna viruses.the ultrastructure and morphogenesis of mason-pfizer monkey virus, isolated from a mammary carcinoma in a rhesus monkey, was compared with those of murine mammary tumor virus, murine leukemia virus, l1210 leukemia-associated virus, and avian myeloblastosis virus. the simian virus resembled murine mammary tumor virus and the l1210 virus in that it produced intracytoplasmic particles that were enveloped during budding. it resembled l1210 virus and murine leukemia virus in budding with smooth envel ...19714327008
the identification of the 3'-hydroxyl nucleoside terminus of avian myeloblastosis virus rna. 19714328813
direction of polymerization by the avian myeloblastosis virus deoxyribonucleic acid polymerase. 19714332562
purification of the dna polymerase of avian myeloblastosis virus. 19714334983
mitochondrial dna from cells transformed by avian myeloblastosis virus. 19714324717
helical nucleocapsid structure of the oncogenic ribonucleic acid viruses (oncornaviruses).negative staining of virions and isolated nucleoids from avian myeloblastosis virus, murine leukemia virus, murine mammary tumor virus, and feline leukemia virus reveals common internal structures. the majority of virions that are penetrated by phosphotungstate show spherical nucleoids with no apparent symmetry. in a small percentage of virions, two distinctive structures are found: (i) single strands (3 to 5 nm in diameter) which are presumed to be the nucleoprotein and are found randomly orien ...19714108573
deoxyribonucleic acid polymerase associated with avian tumor viruses: secondary structure of the deoxyribonucleic acid product.the products of the deoxyribonucleic acid (dna) polymerase associated with rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses with equilibrium centrifugation and stepwise elution from hydroxyapatite. the initial enzymatic product consists of nascent dna chains which are hydrogen-bonded to 70s viral ribonucleic acid (rna), whereas the final enzymatic product is double-stranded dna. appreciable amounts of free single-stranded dna were not detected at any p ...19714322606
[avian myeloblastosis virus and erythroblastosis virus: existence of common nucleotide sequences]. 19724127089
absence of ribonucleic acid methylase in the avian myeloblastosis virus core.n(2)-guanine-ribonucleic acid-methyltransferase, which is associated with avian myeloblastosis virus, is not a component of the viral core.19724113886
reverse transcriptase activity of human acute leukaemic cells: purification of the enzyme, response to amv 70s rna, and characterization of the dna product. 19724117890
surface-active agents for isolation of the core component of avian myeloblastosis virus.sixty-one surface-active agents were evaluated in a procedure designed to assess their ability to remove the envelope from the core component of avian myeloblastosis virus (amv). the procedure consisted of centrifugation of intact amv through a series of sucrose gradients each containing an upper layer of agent at one of eight concentrations between 0.01 and 10%. the effectiveness of an agent in producing amv cores was indicated by (i) the appearance of light-scattering bands in the region of co ...19724112071
viral and cellular dna polymerase: comparison of activities with synthetic and natural rna templates.two dna polymerases purified from normal human lymphocytes are distinguishable from the viral reverse transcriptases of avian myeloblastosis virus and mason-pfizer monkey virus by their relative affinity for select templates. in this respect, the activity of the two normal human lymphocyte polymerases closely resembles the activity of escherichia coli dna polymerase 1. the viral and cellular dna polymerases are equally active with the nonspecific template, poly(ra) . poly(dt). criteria for disti ...19724113240
serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. i. preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase.monospecific antiserum was prepared against purified deoxyribonucleic acid (dna) polymerase from avian myeloblastosis virus (amv). immunodiffusion assay with purified dna polymerase revealed that the anti-dna polymerase serum formed one precipitation band, whereas no reaction with any of the seven major structural proteins of amv was observed. the antiserum also demonstrated enzyme-neutralizing antibody activity that was associated with the immunoglobulin g fraction. there was no difference in t ...19724117965
serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. ii. comparison of avian deoxyribonucleic acid polymerases.monospecific antiserum prepared against the isolated deoxyribonucleic acid (dna) polymerase of avian myeloblastosis virus (amv) neutralized the endogenous ribonucleic acid-instructed dna polymerase activity of detergent-disrupted virus. the viral polymerase was serologically unrelated to the seven major structural polypeptides of amv. furthermore, the viral enzyme was distinguished from normal cellular dna polymerases by serological criteria; thus, antiserum against the viral enzyme neutralized ...19724117966
comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, escherichia coli, and micrococcus luteus. 19724334908
nucleotides at the rna-dna covalent bonds formed in the endogenous reaction by the avian myeloblastosis virus dna polymerase. 19724337028
translation of avian myeloblastosis virus rna in a cell-free lysate of escherichia coli.in a cell-free extract of e. coli, rna from avian myeloblastosis virus directs the synthesis of a protein that is antigenically identical with the group-specific antigen 4, and other proteins, three of which correspond in molecular weight to group-specific antigens 1-3.19724337245
[immunological study of rna of the avian myeloblastosis virus]. 19724338944
early stimulation of dna synthesis in chicken fibroblasts infected by avian myeloblastosis virus. 19724333571
terminal nucleotides of avian myeloblastosis virus rna and of ribosomal rna from chicken leukemic myeloblasts. 19724347181
isolation and characterization of a protein that stimulates dna synthesis from avian myeloblastosis virus.a protein has been isolated from avian myeloblastosis virus that stimulates the rate and yield of dna synthesis primed by viral rna with purified viral polymerase. it specifically affects the viral polymerase and does not stimulate other dna polymerases under the conditions tested. the viral polymerase, in conjunction with this protein, transcribes extended single-stranded regions of dna, and permits the enzyme to initiate synthesis from single-strand breaks in dna.19724340754
distribution of deoxyribonucleic acid complementary to the ribonucleic acid of avian myeloblastosis virus in tissues of normal and tumor-bearing chickens.(3)h-labeled 70s ribonucleic acid (rna) from purified avian myeloblastosis virus (amv) was used as a probe in deoxyribonucleic acid (dna)-rna hybridization experiments to detect the presence of dna complementary to the amv genome in various tissues from noninfected normal chickens and from chickens infected with amv. there was a remarkable constancy in the average cellular concentration of virus-specific dna found in every tissue from the same uninfected chicken, and even in different chickens f ...19724344249
association of an endoribonuclease with the avian myeloblastosis virus deoxyribonucleic acid polymerase. 19724344646
[immunological study of avian myeloblastosis virus rna]. 19724344954
transfer ribonucleic acid synthetase activity associated with avian myeloblastosis virus.avian myeloblastosis virions purified by conventional techniques were shown to be associated with or to contain transfer ribonucleic acid synthetase activity. arginine, tryptophan, cystine, and lysine synthetase activities were observed.19724335517
widespread presence, in chickens, of dna complementary to the rna genome of avian leukosis viruses.dna-rna hybridization experiments have demonstrated the widespread presence in chickens of dna complementary to the rna of avian myeloblastosis virus. all apparently normal chicken embryos, or adult chickens that were tested, contained viral dna in amounts ranging from 1.7 to 4.6 viral genome equivalents per cell. embryos that were negative or positive for the group-specific antigen of avian leukosis viruses contained the same amount of viral dna. embryos from a strain of chickens free of leukos ...19724335067
the genome of rna tumor viruses contains polyadenylic acid sequences.the 70s genome of two rna tumor viruses, murine sarcoma virus and avian myeloblastosis virus, binds to millipore filters in buffer with high salt concentration and to glass fiber filters containing poly(u). these observations suggest that 70s rna contains adenylic acid-rich sequences. when digested by pancreatic rnase, 70s rna of murine sarcoma virus yielded poly(a) sequences that contain 91% adenylic acid. these poly(a) sequences sedimented as a relatively homogenous peak in sucrose gradients w ...19724337237
the 3'-terminal nucleosides of the high molecular weight rna of avian myeloblastosis virus.the rna isolated from avian myeloblastosis virus was fractionated by sucrose density gradient centrifugation. the 3'-oh terminal nucleosides of various fractions were determined by periodate oxidation followed by tritiated borohydride reduction. the 60-70s fraction and the 35s rna derived from it by heating both have adenosine as the major terminal nucleoside, with cytidine as the next most frequent terminal. control samples of trna(met) (f. coli) and 28s ribosomal rna from mouse ascites tumor c ...19724338584
antigenic variation of the avian myeloblastosis virus obtained from chick embryo fibroblasts. 19724343879
reverse transciptase from mason-pfizer monkey tumor virus, avian myeloblastosis virus, and rauscher leukemia virus and its response to rifamycin derivatives. 19724336883
endogenous rna-directed dna polymerase activity in uninfected chicken embryos.early chicken embryos that are either positive or negative for group-specific antigens of avian leukosis viruses contained endogenous rna-directed dna polymerase activity. this endogenous dna polymerase activity was not increased after mixture of soluble dna polymerases isolated from chicken embryos with disrupted chicken embryo cells. the endogenous activity was resistant to treatment with deoxyribonuclease, and the initial rate of dna synthesis was partially resistant to actinomycin d. in cont ...19724338597
rna-primed dna synthesis in vitro.in vitro dna synthesis on single-stranded circular dna can be initiated by rna primers. rna chains are covalently extended by dna polymerase ii from kb cells and dna polymerase i from micrococcus luteus, but not by an rna-dependent dna polymerase from avian myeloblastosis virus. the reaction product consists of dna chains with a piece of rna at their 5'-ends, hydrogen bonded to the template dna. the primer rna is linked to the product dna via a 3':5'-phosphodiester bond, and can be specifically ...19724338598
degradation of dna rna hybrids by ribonuclease h and dna polymerases of cellular and viral origin.ribonuclease h from human kb cells, chick embryos, calf thymus, avian myeloblastosis virus, and rous associated virus specifically degrades the rna of dna.rna hybrids, producing mono- and oligoribonucleotides terminated in 5'-phosphates. the cellular rnase h is an endonuclease, whereas the viral enzyme appears to be an exonuclease. viral dna polymerase and rnase h copurify through all separation steps. therefore, rnase h activity is an intrinsic part of the viral dna polymerase. dna.rna hybrids ...19724343966
[detection of avian myeloblastosis virus in chicken fibroblast cultures treated with dna from virus-producing leukemic cells]. 19724339978
structural studies on avian myeloblastosis virus: rapid purification and quantitation. 19724343190
vsv pseudotype particles with the coat of avian myeloblastosis virus. 19724345003
on the presence of ribosome-like particles in avian myeloblastosis virions (amv) and unusual ultrastructure of their nucleoid. 19724345211
[fractions of 70 s rna from avian myeloblastosis virus, enriched in rapidly hybridizable sequences with cellular dna and associated with poly a]. 19724347535
rna-dependent dna polymerase activity of rna tumor viruses. ii. directing influence of rna in the reaction.the role of ribonucleic acid (rna) in deoxyribonucleic acid (dna) synthesis with the purified dna polymerase from the avian myeloblastosis virus has been studied. the polymerase catalyzes the synthesis of dna in the presence of four deoxynucleoside triphosphates, mg(2+), and a variety of rna templates including those isolated from avian myeloblastosis, rous sarcoma, and rauscher leukemia viruses; phages f2, ms2, and qbeta; and synthetic homopolymers such as polyadenylate.polyuridylic acid. the e ...19724333539
covalent linkage between ribonucleic acid primer and deoxyribonucleic acid product of the avian myeloblastosis virus deoxyribonucleic acid polymerase.initiation of deoxyribonucleic acid (dna) synthesis by the avian myeloblastosis virus dna polymerase was previously suggested to involve a ribonucleic acid (rna) primer, the initial product being a dna molecule joined by a phosphodiester bond to the rna primer. the existence and nature of such an rna-dna joint was investigated by assaying for transfer of a (32)p atom from an alpha-(32)p-deoxyribonucleotide to a 2'(3')-ribonucleotide after alkaline hydrolysis of the polymerase product. such a tra ...197216789134
dna and rna from avian myeloblastosis virus as templates for viral dna polymerase. 197211946381
avian myeloblastosis virus dna polymerase: initiation of dna synthesis and an associated ribonuclease. 19734369800
[resistance of german chicken lines against avian myeloblastosis virus (amv)]. 19734354748
methionine transfer ribonucleic acids of avian myeloblastosis virus.chick-embryo cells contain four isoaccepting species of methionine transfer rna (i-iv). one species (i) is the initiator, trna(f) (met), and the others (ii, iii, and iv) are the donors of internal methionyl residues (trna(m) (met)). over 85% of the trna(met) in purified avian myeloblastosis virus consists of one trna(m) (met) species, which resembles host-cell trna(met) iv with respect to chromatographic properties on rpc-5, electrophoretic mobility of the terminal methionyl-oligonucleotide, and ...19734355368
separation of dna sequences complementary to the rna of avian myeloblastosis virus from chicken dna by alkaline cesium chloride density sedimentation.density gradient sedimentation in alkaline cesium chloride of dna from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13s revealed that the dna sequences complementary to 70s avian myeloblastosis virus rna sedimented in the high guanine plus cytosine region ahead of the main peak of cellular dna. when the dna was fragmented into pieces of 6.6s there was a broader distribution of the dna sequences complementary to the viral rna. this technique could be employed as a step t ...19734355853
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