Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| hybridoma cell lines secreting monoclonal antibodies against equine infectious anemia virus. | a monoclonal anti-equine infectious anemia virus (anti-eiav) antibody (1b15) has been generated by fusion of x63 ag 8.653 myeloma cells and spleen cells from mice hypersensitized with viral antigen p29. ouchterlony double-diffusion analysis indicated that antibody 1b15 is of the igg class. the specificity of the immune reaction for p29 was confirmed by cross-over immunoelectrophoresis and disc-gel electrophoresis. mab 1b15 was used to devise a solid-phase 'capture' ria for eiav-p29 antigen. the ... | 1987 | 2435751 |
| lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus. | the nucleotide sequence of the envelope (env) gene region of equine infectious anemia virus (eiav), a member of the lentivirus subfamily of retroviruses, has been determined from a clone of integrated proviral dna for which the gag and pol sequences have been reported previously. the env gene is 859 codons in length and the sequence reported here is consistent with the published biochemical properties of eiav glycoproteins. the env gene region of eiav shares considerable structural similarities ... | 1986 | 2431539 |
| lav/htlv-iii gag gene product p24 shares antigenic determinants with equine infectious anemia virus but not with visna virus or caprine arthritis encephalitis virus. | antigenic cross-reactivity of the human acquired immunodeficiency disease syndrome virus lav/htlv-iii with the lentiviruses visna virus, caprine arthritis encephalitis virus (caev), and equine infectious anemia virus (eiav) was determined with indirect enzyme-linked immunosorbent assays, immunoblot analysis, and virus-specific polyclonal antisera. nonreciprocal cross-reactivity was seen between the gag gene products p24 of lav/htlv-iii and p28 of eiav. reciprocal cross-reactivity was seen betwee ... | 1986 | 2438251 |
| reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis. | we describe a staining technique, using ponceau s in very mild conditions, by which proteins can be visualized on nitrocellulose replicas without being permanently fixed to the membrane itself, thus allowing subsequent procedures such as immunoblotting or preparative elution of the proteins to be performed. this staining technique can detect 250 to 500 ng protein, which is essentially the same sensitivity seen for coomassie blue staining of proteins on nitrocellulose. the ponceau s staining tech ... | 1986 | 2429581 |
| shedding and interspecies type sero-reactivity of the envelope glycopolypeptide gp120 of the human immunodeficiency virus. | two glycopolypeptides with molecular weights 160,000 and 120,000 (gp120) are regularly recognized by human immunodeficiency virus (hiv)-specific antisera in lysates of cells persistently infected with hiv. in the present study, gp120 was characterized as the major envelope glycopolypeptide of hiv. gp120 was identified as the external viral glycoprotein by radiosequencing and by its presence in purified virus. however gp120 was predominantly shed as a soluble protein into the culture fluid. furth ... | 1986 | 2431105 |
| isolation of a lentivirus from a macaque with lymphoma: comparison with htlv-iii/lav and other lentiviruses. | a retrovirus has been isolated on the human t-cell line hut 78 after cocultivation of a lymph node from a pig-tailed macaque (macaca nemestrina) that had died with malignant lymphoma in 1982 at the university of washington primate center. this isolate, designated mniv (wprc-1) (m. nemestrina immunodeficiency virus, washington primate research center) shows the characteristic morphology of a lentivirus and replicates to high titers in various lymphocyte lines of human and primate origin. sodium d ... | 1986 | 3021982 |
| molecular cloning and physical characterization of integrated equine infectious anemia virus: molecular and immunologic evidence of its close relationship to ovine and caprine lentiviruses. | molecular clones of the integrated form of the genome of equine infectious anemia virus (eiav), the etiologic agent of a naturally occurring, worldwide disease of horses, were obtained. the restriction map of a full-length genome was determined. additional evidence for the close evolutionary relationship between eiav and a prototype lentivirus (caprine arthritis encephalitis virus) was acquired by southern blotting and immunological analyses. an interspecies radioimmunoassay was developed in whi ... | 1986 | 3750842 |
| equine infectious anemia virus gag and pol genes: relatedness to visna and aids virus. | comparison of htlv-iii, the putative aids virus, with other related viruses, may help to reveal more about the origin of aids in humans. in this study, the nucleotide sequence of the gag and pol genes of an equine infectious anemia virus (eiav) proviral dna clone was determined. the sequence was compared with that of htlv-iii and of visna, a pathogenic lentivirus of sheep. the results show that these viruses constitute a family clearly distinct from that of the type c viruses or the blv-htlv-i a ... | 1986 | 3003905 |
| rapid emergence of novel antigenic and genetic variants of equine infectious anemia virus during persistent infection. | previous results from our laboratory have demonstrated that equine infectious anemia virus displays structural variations in its surface glycoproteins and rna genome during passage and chronic infections in experimentally infected shetland ponies (montelaro et al., j. biol. chem. 259:10539-10544, 1984; payne et al., j. gen. virol. 65:1395-1399, 1984). the present study was undertaken to obtain an antigenic and biochemical characterization of equine infectious anemia virus isolates recovered from ... | 1986 | 3001367 |
| the persistent infection of a canine thymus cell line by equine infectious anaemia virus and preliminary data on the production of viral antigens. | equine infectious anaemia virus (eiav) was adapted to the cf2th cell line, a heterologous malignant line from canine thymus. a persistent infection was monitored for 100 serial passages by demonstrating the presence of virus and viral antigens at each 10th passage by electron-microscopy, immunodiffusion and immunofluorescence. chromosome analysis of eiav-infected cells indicated they had a karyotype resembling the control cells of similar passage history. virus-infected cells, grown in roller cu ... | 1986 | 3018020 |
| comparison of glycoproteins by two-dimensional mapping of glycosylated peptides. | we describe here a two-dimensional mapping procedure which is capable of resolving glycopeptides isolated by lectin affinity chromatography from radioiodinated tryptic digests of glycoproteins. glycopeptide maps were successfully produced for the model proteins alpha 1-acid glycoprotein and fetuin, as well as for the two surface glycoproteins gp90 and gp45 from equine infectious anemia virus (eiav). differences were detected in the glycopeptide maps obtained for the gp90 and gp45 components from ... | 1986 | 3021020 |
| nucleotide sequence and transcriptional activity of the caprine arthritis-encephalitis virus long terminal repeat. | caprine arthritis-encephalitis virus (caev) and visna virus are pathogenic lentiviruses of goats and sheep which share morphologic features and sequence homology with human t-cell lymphotropic virus type iii (htlv-iii), the etiologic agent of the acquired immune deficiency syndrome. the nucleotide sequence of the caev long terminal repeat (ltr) was determined, and it was found to be 450 base pairs long, with u3, r, and u5 regions of 287, 85, and 78 base pairs, respectively. portions of the caev ... | 1986 | 3021973 |
| rapid solid-phase radioimmunoassay for detection of equine infectious anemia viral antigen and antibodies: parameters involved in standardization. | solid-phase radioimmunoassays (spria) are described for the detection of equine infectious anemia (eia) viral antigen and antibodies. protein-antigen p29 currently used in the agar-gel immunodiffusion (agid) test was used as antigen in the spria. rabbit sera selected from positive agid test data were used to standardize the method. briefly, wells of flexible microtitre plates coated with antigen were incubated with antiserum followed by a secondary labelled antibody. the radioactivity remaining ... | 1985 | 3001113 |
| equine infectious anemia virus: immunopathogenesis and persistence. | equine infectious anemia (eia) is a chronic, relapsing infectious disease of horses caused by a nononcogenic retrovirus. virus persists in infected animals for life and can be reliably detected by serologic tests that measure levels of antibody to the major structural protein of the virus. periodic virus replication in macrophages leads to an immunologically mediated acute disease characterized primarily by severe anemia. recrudescence of acute eia is the result of antigenic variation of the sur ... | 1985 | 2984759 |
| prospective study of progeny of inapparent equine carriers of equine infectious anemia virus. | progeny of a band of horses, positive by the agar-gel immunodiffusion (agid) test for equine infectious anemia (eia) antibody, were observed through their weaning over a 4-year period. sentinels (agid test-negative) were allowed to mingle with eia-infected mares and their foals in pasture situations in an area with high populations of potential vectors. of 27 adult sentinels, 8 (30%) seroconverted in annual rates ranging from 0% to 75%. in contrast, only 2 of 31 (6%) foals weaned became infected ... | 1985 | 2988379 |
| nucleotide sequence evidence for relationship of aids retrovirus to lentiviruses. | lentiviruses are a subfamily of retroviruses which have been aetiologically linked to the induction of arthritis, encephalitis, progressive pneumonia and slow neurological diseases in certain species. relatively little is known about their genome structure, mechanisms of pathogenesis or evolutionary relationships with other retroviral subfamilies. in an effort to understand better the mechanisms by which these viruses induce such a variety of chronic diseases, we have molecularly cloned and phys ... | 1985 | 2995822 |
| lymphadenopathy-associated virus: from molecular biology to pathogenicity. | recent data indicate that the lymphadenopathy-associated virus (lav) is morphologically similar to animal lentiviruses, such as equine infectious anemia and visna viruses. this finding, together with the cross-reactivity of the core proteins of lav with those of the equine infectious anemia virus and a similarity in genome structure and biological properties, allows lav to be placed in the retroviral subfamily of lentivirinae. molecular data indicate a high degree of genetic variation of the vir ... | 1985 | 2996400 |
| observations of tabanid feeding on mares and foals. | the occurrence of tabanid feeding between mares and foals was observed. when mares and foals were observed freely moving within a pasture situation, foals had 2.43% (4 flies in 77 observations vs 297 flies in 139 observations) of the tabanid feeding occurrences of the mares. this difference in tabanid burden varied due to herd size, herd location, and tabanid species. lower tabanid burden of foals was indicated as a practical protective mechanism against pathogenic agents mechanically transmitte ... | 1985 | 4003887 |
| human t-cell lymphotropic virus type iii: immunologic characterization and primary structure analysis of the major internal protein, p24. | the major internal structural protein of human t-cell lymphotropic virus type iii (htlv-iii), a virus etiologically implicated in acquired immunodeficiency syndrome (aids), was purified to homogeneity. this 24,000-molecular-weight protein (p24) was shown to lack immunologic cross-reacting antigenic determinants shared by other known retroviruses, including htlv-i and htlv-ii, with the exception of equine infectious anemia virus (eiav). a broadly reactive competition immunoassay was developed in ... | 1985 | 2410630 |
| enzyme-linked immunosorbent assay for detection of equine infectious anemia antibody to purified p26 viral protein. | an indirect enzyme-linked immunosorbent assay (elisa) was developed for the detection of equine infectious anemia (eia) antibody in horse sera. purified p26 viral protein was the antigen; alkaline phosphatase linked to rabbit anti-horse immunoglobulin g was the conjugate. the elisa detected eia antibodies in horse sera as early as 11 to 14 days after experimental inoculations. there was full agreement between the results of elisa and the agar-gel immunodiffusion tests on eia proficiency test ser ... | 1984 | 6089620 |
| antigenic reactivity of the major glycoprotein of equine infectious anemia virus, a retrovirus. | the immunogenic contributions of the carbohydrate and peptide portions of the major envelope glycoprotein of equine infections anemia virus, eiav gp90, were analyzed by measuring the effects of specific glycosidase and protease digestions on the reactivity of the glycoprotein with immune sera from infected horses. the results of both direct and competitive radioimmunoassay demonstrated that immune sera contained antibodies reactive with both the carbohydrate and protein moieties of eiav gp90, wi ... | 1984 | 6205503 |
| genomic alterations associated with persistent infections by equine infectious anaemia virus, a retrovirus. | the unique periodic nature of equine infectious anaemia (eia) is believed to result from the ability of the infecting virus. eiav, to undergo relatively rapid antigenic variations which circumvent host immune responses resulting in distinct virus populations in sequential clinical episodes in the persistently infected horse. this model was examined by oligonucleotide mapping comparisons of the rna genomes of selected isolates of eiav. variations in oligonucleotide maps could be reproducibly demo ... | 1984 | 6086822 |
| cytoplasmic inclusions in cells infected with the virus of equine infectious anemia (eiav). | after infection with equine infectious anemia virus (eiav) equine dermal (ed) cells revealed fluorescent spots in their cytoplasm which were detected with an indirect immunofluorescence technique. to characterize the nature of these cytoplasmic inclusions, comparative light and electron microscopic studies were performed. the cytoplasmic location of the spots observed in the indirect immunofluorescence assay coincided with the location of structures detected with the electron microscope. in ultr ... | 1984 | 6325195 |
| detection of equine infectious anemia virus in horse leukocyte cultures derived from horses in various stages of equine infectious anemia viral infection. | the enzyme-linked immunosorbent assay (elisa) antigen-positive and agar-gel immunodiffusion test (agid)-negative horses do not have infective equine infectious anemia (eia) virus. the elisa testing of horse leukocyte culture (hlc) supernatants did detect eia virus in a hlc that was infected with the wyoming strain of eia virus and in hlc derived from horses in febrile, acute, or subacute stages of eia infection. in supernatants of hlc derived from chronic and inapparent carrier horses, eia virus ... | 1984 | 6322623 |
| antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus. | the recurrent nature of equine infectious anemia has been attributed to relatively rapid antigenic variations in equine infectious anemia virus (eiav) during persistent infection under selective immune pressures. this model was tested by serological and biochemical analysis of virus isolates recovered from separate febrile episodes in two experimentally infected ponies. neutralization assays employing immune sera from the experimentally infected ponies demonstrated that distinct antigenic strain ... | 1984 | 6206055 |
| transmission and clinical evaluation of an equine infectious anemia herd and their offspring over a 13-year period. | 1984 | 6321418 | |
| an overview of equine infectious anemia control and regulation in the united states. | 1984 | 6321419 | |
| structural proteins of equine infectious anemia virus and their antigenic activity. | using purified equine infectious anemia (eia) virus labeled with 3h-glucosamine or 14c-protein hydrolysate, structural proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. as a result, 2 glycoproteins and 10 proteins with molecular weights (mol wt) ranging from 12,000 to 115,000 daltons were demonstrated. of 12 structural proteins, 3 proteins, namely a glycoprotein with mol wt of 76,000 (gp76) and 2 proteins with mol wt of 25,000 (p25) and 12,000 (p12), respective ... | 1984 | 6322625 |
| enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody. | a sensitive specific enzyme-linked immunosorbent assay utilizing purified p26 antigen was developed for the detection of antibodies to equine infectious anemia virus in naturally and experimentally infected horses. generally, antibodies to the virus could be detected by the enzyme-linked immunosorbent assay 3 to 4 days earlier than by the standard agar gel immunodiffusion test, and they could be detected more reliably in horses with weak or equivocal agar gel immunodiffusion test reactions. the ... | 1984 | 6325488 |
| characterization of monoclonal antibodies directed against the envelope proteins of feline leukemia virus. | monoclonal antibodies directed against the feline leukemia virus (felv) envelope proteins, gp70 and p15e, were identified by radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. six of these monoclonal antibodies were specific for the gp70; two for the p15e. enzyme-linked immunosorbent assay binding assays against felv subtypes a, b, and c showed that most of the monoclonal antibodies bound to more than one subtype but have a greater affinity for subtype b. one ... | 1984 | 6331649 |
| carriers of equine infectious anemia virus. | presently available data continue to support the idea that once a horse is infected with equine infectious anemia virus it remains infected indefinitely. infection may not always be demonstrated by inoculation of plasma, serum, or whole blood transfusions into susceptible recipients, but transfusions of fresh whole blood will be infective in at least 95% of the horses testing positive in the agar gel immunodiffusion test. for detection of infectivity in a small percentage of inapparent carriers, ... | 1984 | 6421787 |
| studies on equine infectious anemia virus transmission by insects. | there are several factors involved in the mechanical transmission of equine infectious anemia (eia) virus by insects. large hematophagous insects, especially tabanids, which feed from extravascular sites (ie, pool feeding) appear to be the most efficient vectors. the biology of the host-seeking and blood-feeding behavior of the vectors are important variables that have been overlooked in the mechanical transmission of pathogens like eia virus. the biology, population levels, and diversity of the ... | 1984 | 6321420 |
| mechanical transmission of equine infectious anemia virus by deer flies (chrysops flavidus) and stable flies (stomoxys calcitrans). | 1983 | 6297339 | |
| isolation of equine infectious anemia virus glycoproteins. lectin affinity chromatography procedures for high avidity glycoproteins. | lectin affinity chromatography procedures were evaluated for the isolation of enveloped virus glycoproteins. the major glycoprotein of equine infectious anemia virus (eiav) bound to concanavalin a (con a)-sepharose through interactions which could not be reversed by alpha-methylglucoside, but elution could be accomplished with buffers containing guanidine hydrochloride or sodium dodecyl sulfate. these denaturants, however, also released about one-half of the con a protein from the sepharose matr ... | 1983 | 6309879 |
| effects of common radioiodination procedures on the binding of glycoproteins to immobilized lectins. | representative glycoproteins including fetuin, protein a, ovalbumin, alpha 1 acid glycoprotein, and the major glycoprotein of equine infectious anemia virus were labelled with 125i by the chloramine-t or bolton-hunter procedure and their binding to immobilized con a or lentil lectin compared to untreated samples of each glycoprotein. glycoprotein modification was no greater than one substituted residue per protein molecule. yet the radioiodinated glycoproteins typically displayed only 0-50% of t ... | 1983 | 6838504 |
| persistent infection by equine infectious anemia virus: asymmetry of nucleotide sequence reiteration in the integrated provirus of persistently infected cells. | 1982 | 6281971 | |
| transmission of equine infectious anemia virus from horses without clinical signs of disease. | twenty seven adult horses positive to the agar gel immunodiffusion (agid) test for equine infectious anemia (eia), but with no history of clinical eia, were used in transfusion studies to determine whether infectious eia virus was present in 1 to 5 ml of their blood. of 27 recipients, 21 (78%) became agid test-positive at an average of 24 days after inoculation. two horses that were initially negative when screened were retested and found to carry infectious virus in 5-300 ml of whole blood; the ... | 1982 | 6276353 |
| detection of equine infectious anemia virus in a horse with an equivocal agar gel immunodiffusion test reaction. | a horse whose serum reacted equivocally in the agar gel immunodiffusion (agid) test for equine infectious anemia was studied over a 3-year period. the horse remained afebrile and virus was detected in only 1 of 6 horse inoculation tests. the intensity of agid test reactions increased temporarily following this evidence for virus. although the agid test reaction was equivocal and 5 of the 6 transmission attempts failed, the 1 successful transmission proved the horse was infected. | 1982 | 6276354 |
| indirect hemagglutination test in equine infectious anemia. | an indirect hemagglutination was developed for the diagnosis of equine infectious anemia using sheep red blood cells coated with group specific virus antigen which had been highly purified by affinity chromatography. the presence of indirect hemagglutination antibodies was demonstrated in horses with equine infectious anemia since the cells were specifically agglutinated by all the serum samples obtained from experimentally infected horses. antibodies appeared within 35 days after inoculation, a ... | 1982 | 6280821 |
| antigenic stimulation of t lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia. | equine infectious anemia is a chronic disease of horses caused by a nononcogenic retrovirus. studies were undertaken to determine the types of cells involved in the in vitro lymphoproliferative response to viral antigens and the dynamics of this reaction. it was observed that reactive lymphocytes were present at unpredictable times in the peripheral blood of infected horses. this reaction was shown to be specific for the interaction of equine infectious anemia virus and t lymphocytes. enriched b ... | 1982 | 6281191 |
| dna sequence relationship of the baboon endogenous virus genome to the genomes of other type c and type d retroviruses. | baboon endogenous virus (baev) is a type c retrovirus present in multiple proviral copies in the dna of baboons. although interspecies antigenic determinants present on reverse transcriptase and gag proteins are shared among all mammalian type c viruses, no nucleic acid homology between baev and other type c viruses (except rd-114) has been found in conventional liquid hybridization experiments. in this study, we used restriction fragments of cloned baev dna immobilized on nitrocellulose to test ... | 1982 | 6284972 |
| virulence and in vitro growth of a cell-adapted strain of equine infectious anemia virus after serial passage in ponies. | five serial passages of a cell-adapted strain of equine infectious anemia (eia) virus were conducted in shetland ponies. the 13 recipient ponies became agar-gel immunodiffusion test-positive by 25 days after they were inoculated. the virulence of the cell-adapted strain of eia virus markedly increased through 3 serial passages, although individual variation within passages was high. the 1st serial-passage recipient remained afebrile through 200 days, whereas a febrile episode occurred about ever ... | 1982 | 6293349 |
| equine infectious anaemia: detection of antibodies using an immunofluorescence test. | an indirect immunofluorescence test for detecting antibodies to equine infectious anaemia virus is presented. using monolayers of equine dermal cells within a defined period after infection, discrete fluorescent spots were observed in the cytoplasm of as many as 95 per cent of the cells. these inclusions appeared as ring-like structures when high titred sera were employed but became spots when the sera were diluted. cells showing optimal antigen fluorescence were used immediately or after storag ... | 1982 | 6296954 |
| isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus. | we describe procedures for the large-scale production of equine infectious anemia virus (eiav) and for the isolation of the four major non-glycosylated virion proteins, designated p26, p15, p11, and p9. comparisons of the purified proteins by peptide mapping procedures and by enzyme-linked immunosorbent assays demonstrated the unrelatedness of the four proteins. the characteristic properties of each purified protein were examined by determining isoelectric points and amino acid compositions. we ... | 1982 | 6178843 |
| in vitro host range of equine infectious anemia virus. | equine infectious anemia virus (eiav) was successfully inoculated onto cell cultures of canine and feline origin, resulting in chronic infections in these cultures. infection of equine cell cultures, which were the previous sole in vitro source demonstrated for virus production, was also performed for comparative purposes. determination of the nature of the virus produced in the heterologous as well as the equine cells was accomplished in several ways. sds-page of purified virus from the differe ... | 1981 | 6177659 |
| hemagglutination-inhibition tests with different strains of equine infectious anemia virus. | the serologic relationships between 6 strains of equine infectious anemia (eia) viruses were investigated by hemagglutination-inhibition (hi) tests. cross hi tests, using sera from horses in the early stage of infection, revealed that all strains were inhibited only by homologous strain antisera and that hi antibody was always detectable before virus-neutralizing antibody. in the later stages of infection, both homologous and heterologous hi antibodies were detected in a sera of most of the hors ... | 1981 | 6175255 |
| transmission of equine infectious anaemia virus from a horse negative to agar gel immunodiffusion testing. | 1981 | 6265206 | |
| the immune system in slow disease due to persistent submicrobial (viral) infection. | 1981 | 6270625 | |
| prevalence of equine infectious anaemia (swamp fever) in guyana. | 1981 | 6272928 | |
| hemagglutination of several strains of equine infectious anemia virus. | six strains of equine infectious anemia (eia) virus propagated in equine leukocyte cultures were found to agglutinate horse erythrocytes. concentrated virus material containing about 20 units of complement fixation (cf) titer showed hemagglutinating (ha) titers ranging from 4 to 8 units. the ha activity remained stable after ether treatment and was reduced by trypsin, formaldehyde and kio4. cesium chloride equilibrium density gradient centrifugation revealed two populations of hemagglutinin, one ... | 1981 | 6263225 |
| propagation of equine infectious anemia virus in horse cell cultures. | 1981 | 6272480 | |
| high-performance gel permeation chromatography of proteins in denaturing solvents and its application to the analysis of enveloped virus polypeptides. | 1981 | 6272599 | |
| serological survey for equine infectious anaemia. | 1981 | 6275830 | |
| propagation of equine infectious anemia virus in horse cell cultures. | the wyoming strain of equine infectious anemia virus was adapted to cell cultures by 7 passages in horse leukocytes and 14 passages in fetal equine dermal and kidney cells. the virus was made evident by electron microscopy and immunodiffusion tests with antigens prepared from culture fluids. | 1981 | 6306910 |
| studies with equine infectious anemia virus: transmission attempts by mosquitoes and survival of virus on vector mouthparts and hypodermic needles, and in mosquito tissue culture. | biological and mechanical transmission trials with psorophora columbiae (dyar and knab) and aedes sollicitans (walker) and ponies acutely infected with equine infectious anemia virus (eiav) were negative. the eiav antigen was detected by radioimmunoassay in ae sollicitans immediately after the mosquitoes had fed on an acutely ill pony, but not 14 days after feeding. psorophora columbiae mosquitoes had detectable eiav antigen as determined by radioimmunoassay 24 hours after they fed on an acutely ... | 1981 | 6119953 |
| [comparative investigations on the serological diagnosis of the equine infectious anemia (author's transl)]. | 1981 | 6262037 | |
| the role of stable flies and mosquitoes in the transmission of equine infectious anemia virus. | 1980 | 6118874 | |
| equine infectious anemia virus, a putative lentivirus, contains polypeptides analogous to prototype-c oncornaviruses. | 1980 | 6256947 | |
| orientation of structural polypeptides of equine infectious anemia virus. | lactoperoxidase iodination of intact and disrupted equine infectious anemia virus revealed that glycopeptide gp79 is the major surface component of this virus, whereas glycopeptides gp64 and gp40 as well as the principle nonglycosylated structural polypeptides p29 and p13 are internal. virus 'envelope' particles, banding in isopycnic centrifugation at approximately 1.10 g/cm3, contained the glycopeptides but no internal nonglycosylated proteins. glycopeptides gp79, gp64 and gp40 and core polypep ... | 1980 | 6259082 |
| isolation and characterization of low-molecular-weight dna-binding proteins from retroviruses. | 1980 | 6249039 | |
| equine infectious anemia: current knowledge. | 1979 | 218920 | |
| equine infectious anemia virus: development of a simple reproducible method for titrating infectivity of the cell-adapted strain. | an equine dermal cell line between the 14th and 30th subpassages was used to develop a reproducible method of titrating the infectivity of the cell-adapted strain of equine infectious anemia virus (eiav). cells inoculated with eiav were subcultured or fed once each week and were monitored for the production of p26 antigen of eiav in supernatant fluids. ultrathin sections were prepared once each week and were examined for the detection of budding virus-like particles (vlp). the vlp could not be d ... | 1979 | 223479 |
| leukocyte cytotoxicity in a persistent virus infection: presence of direct cytotoxicity but absence of antibody-dependent cellular cytotoxicity in horses infected with equine infectious anemia virus. | antibody-dependent cellular cytotoxicity and direct cytotoxicity assays were performed with equine infectious anemia virus-infected target cells, equine leukocytes, and equine anti-equine infectious anemia virus antibody to determine whether these mechanisms play a role in controlling viral replication in equine infectious anemia. direct cytotoxicity was observed by using peripheral blood mononuclear cells from 7 of 10 infected horses. antibody-dependent cellular cytotoxicity was not observed. t ... | 1979 | 223981 |
| responses in horses infected with equine infectious anemia virus adapted to tissue culture. | 1979 | 228570 | |
| characterization of the infection of equine fibroblasts by equine infectious anemia virus. | equine dermal fibroblasts persistently infected with equine infectious anemia virus (eiav) show no alterations in cell morphology or growth kinetics when compared to uninfected cells. the percentage of cells immunofluorescent positive for viral proteins fluctuated, depending upon the stage of the cell cycle, while production of extracellular virus was uniform throughout the cell cycle, increasing only as the cell number increased. this was shown in log versus stationary phase cultures as well as ... | 1979 | 228638 |
| synthesis of long complementary dna in the endogenous reaction by equine infectious anemia virus. | in the endogenous reverse transcriptase reaction, equine infectious anemia virus is able to synthesize complementary dna (cdna) of 8,000 nucleotides in high yield. after 2 h in 50 mum dntp, about 2.8 mug of cdna per mg of protein is produced, almost 30% of which is long cdna. the system thus compares favorably with the other two well-characterized endogenous reaction systems, moloney murine leukemia virus and avian sarcoma virus. elongation rates of 100 to 150 nucleotides per min have been obser ... | 1979 | 87522 |
| immunology of a persistent retrovirus infection--equine infectious anemia. | 1979 | 95154 | |
| prevalence of antibodies to equine viruses in the netherlands. | the prevalence of antibodies to various viruses was investigated in a series of serum samples collected from horses in the netherlands between 1963 and 1966 and from 1972 onwards. neutralizing antibodies to equine rhinopneumonitis virus, equine arteritis virus and to equine rhinovirus types 1 and 2 were detected in respectively 76%, 14%, 66% and 59% of the equine serum samples tested. the observed incidence of serum samples positive to equine adenovirus in the complement fixation test was 39%. p ... | 1979 | 219560 |
| serologic survey for equine infectious anemia virus in louisiana. | in 1975, a survey was conducted in east baton rouge parish, louisiana, to determine the prevalence of equine infectious anemia. using the agar gel immunodiffusion test, 94 of 1,398 horses (6.7%) were found to be infected. infection rates were especially high in areas where clinical cases of equine infectious anemia had been diagnosed. clinical signs compatible with the disease were noted in 1 of the 94 seropositive horses. the sample set of 1,398 horses represented 22% of the census population o ... | 1979 | 221447 |
| specificity of response to viral proteins in horses infected with equine infectious anemia virus. | three structural proteins of equine infectious anemia virus were purified, labeled with 125i, and utilized in radioimmunoassays with horse sera and antisera to heterologous retroviruses. whereas radioimmunoassay titers for the major protein, p25, were 500- to 1,000-fold higher than titers in immunodiffusion, for clinical purposes these two procedures were equivalent. antibodies to two low-molecular-weight proteins, p12 and p10, were also found in infected horses, but with a lower frequency and l ... | 1979 | 217831 |
| scanning and transmission electron microscopic study of equine infectious anemia virus. | scanning and transmission electron microscopy were used to study in detail the morphogenesis and replication of equine infectious anemia virus (eiav) in cultured, persistently infected equine fetal kidney fibroblasts. the eiav was shown by thin-section electron microscopy to resemble morphologically more closely the members of the genus lenti-virus in the family retroviridae than other genera. scanning electron microscopy demonstrated budding virus on only about 5% of the equine fetal kidney fib ... | 1978 | 215061 |
| induction of a cell membrane antigen by equine infectious anemia virus. | equine fibroblasts persistently infected with equine infectious anemia virus acquire a new cell membrane antigen demonstrable by indirect radioimmunoassay, using infected horse serum as an antibody source. | 1978 | 205145 |
| the structural polypeptides of equine infections anemia virus. | the structural polypeptides and glycoproteins of equine infectious anemia virus were identified following electrophoresis in sds-page. the major non-glycosylated polypeptides had molecular weights of 25,000, 14,000 and 11,000 daltons. two glycoproteins of 80,000 and 40,000 daltons were also detected. the relationship of these components to the structural elements of mammalian c-type oncoviruses is discussed. | 1978 | 202575 |
| preparation of hemagglutinating antigen of equine infectious anemia virus from infected equine leukocyte cultures. | 1978 | 206841 | |
| [cytomorphological aspects of the blood experimentally induced with inoculation of equine infectious anemia virus]. | 1978 | 208135 | |
| detection of proviral dna in horse cells infected with equine infectious anemia virus. | equine infectious anemia virus (eiav) recently has been shown to possess a high-molecular-weight rna genome and a virion reverse transcriptase. we completed the demonstration that eiav is a retrovirus by showing the presence of proviral dna in equine cells infected in vitro, but not in normal horse dna. these studies were performed by using a highly representative cdna probe synthesized by the virion polymerase. it was found that this cdna reassociated extensively, and with high thermal stabilit ... | 1978 | 209211 |
| simple method for preparation of specific antisera against viral proteins: rabbit antisera against equine infectious anemia virus proteins p26 and p16. | a simple method for preparation of highly specific antisera against equine infectious anemia virus proteins p26 and p16 is described. viral proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the motility of the viral proteins in the gel was compared with standards. unstained portions of the slab gel were sliced into 5-mm bands, emulsified with freund's complete adjuvant, and injected into rabbits to produce specific antisera. | 1978 | 216292 |
| role of horse flies in transmission of wquine infectious anemia from carrier ponies. | equine infectious anemia virus was transmitted from an acutely ill and an inapparently infected pony to uninfected ponies by the interrupted feeding of horse flies (tabanids). transmission from acutely ill ponies was not accomplished following: (1) the interrupted feeding of a single horse fly, (2) bites of horse flies that had fed on an acutely affected pony 24 hours earlier, (3) bites of horse flies that had oviposited after feeding on an acutely affected pony, or (4) the inoculation of larval ... | 1978 | 621184 |
| structural proteins of equine infectious anemia virus. | equine infectious anemia virus was found to be comprised of fourteen polypeptides of molecular weight ranging from 10,000 to 79,000. eighty percent of the virion protein was accounted for by five polypeptides, including two non-glycosylated components (p29 and p13) comprising one-half of the virion protein and three glycoproteins (gp77/79, gp64, and gp40). | 1978 | 215790 |
| failure to propagate equine infectious anemia virus in mosquitoes and culicoides variipennis. | laboratory-colonized mosquitoes, culex tarsalis, aedes aegypti, culiseta inornata, and anopheles free-borni, and the biting gnat, culicoides variipennis, were exposed to equine infectious anemia virus. exposure to the virus was by intrathoracic inoculation for mosquitoes and by oral ingestion of an infective blood meal through a membrane for c variipennis. after various intervals, groups of 15 to 20 insects were homogenized and inoculated into susceptible ponies. positive immunodiffusion test r ... | 1978 | 31831 |
| inactivation of equine infectious anemia virus by chemical disinfectants. | twelve chemicals and commercial disinfectants were tested for inactivation of equine infectious anemia virus. in the presence of 10% bovine serum, all chemicals inactivated 4 log10 (based on 0.1 ml) of the virus within 5 minutes at 23 c. a reduction of at least 4 log10 was observed when the virus was exposed for 1 minute to substituted phenolic disinfectants (3 commercial preparations and sodium orthophenylphenate), halogen derivatives (iodophor and sodium hypochlorite), chlorhexidine, and 70% e ... | 1977 | 199094 |
| immunoglobulin g subclass [igg and igg(t)] interaction with the p26 group specific antigen of equine infectious anemia virus: immunodiffusion and complement-fixation reactions. | isolated equine immunoglobulin (ig)g(t) antibodies to equine infectious anemia virus p26 antigen did not precipitate with antigen when the ratio of antibody to antigen was high. however, at lower ratios of antibody to antigen precipitation occurred. in addition, complement-fixation by igg and p26 antigen was inhibited by high concentrations of igg(t). the unusual reaction pattern noted with igg(t) antibodies was still detectable by the immunodiffusion test for equine infectious anemia virus. in ... | 1977 | 195493 |
| equine antibody to bovine serum induced by several equine vaccines as a source of extraneous precipitin lines in the agar gel immunodiffusion test for equine infectious anemia. | precipitin lines not associated with equine infectious anemia (eia) were observed in routine agar gel immunodiffusion (agid) testing for the infection. the serums which produced these lines were obtained from horses which had been given multiple vaccinations with commercially available cell culture-origin equine virus vaccines as part of a comprehensive herd health program. the lines formed against cell culture-derived, but not spleen-derived eia viral antigens. investigation revealed that bovin ... | 1977 | 192111 |
| characterization of a retravirus isolated from squirrel monkeys. | a new retravirus (smrv) isolated from a squirrel monkey, saimiri sciureus, has an mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride, similar to the mouse mammary tumor virus and the mason-pfizer monkey virus. the polypeptide patter of smrv as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was distinct from the reported polypeptide patterns of known retraviruses. four major polypeptides of molecular weight ... | 1977 | 69728 |
| purification of equine infectious anemia virus antigen by affinity chromatography. | affinity chromatography was performed to obtain highly purified antigen from equine infectious anemia (eia) virus. after crude antigen was concentrated by polyethylene glycol precipitation of culture fluids from equine dermal cells persistently infected with eia virus, and after the virus was disrupted with ether, it was added to a column of cyanogen bromide-activated sepharose 4b to which eia-specific antibody had been conjugated. the antigen was effectively released from the column with 5m mgc ... | 1977 | 69631 |
| rna-dependent dna polymerase associated with equine infectious anemia virus. | equine infectious anemia (eiav) is shown to have an associated rna-instructed dna polymerase similar in its cofactor requirements and reaction conditions to the rna tumor virus dna polymerases. demonstrating this dna polymerase activity requires a critical concentration of a nonionic detergent, all four deoxyribonucleoside triphosphates, and a divalent metal ion. the reaction is sensitive to rnase, and a substantial fraction of the fna synthesized is complementary to viral rna. the detection of ... | 1977 | 67219 |
| characterization of rna from equine infectious anemia virus. | the genome of equine infectious anemia virus, a nononcogenic retrovirus, has been characterized by velocity sedimentation, electrophoresis in polyacrylamide gels, buoyant density in cs2so4, and susceptibility to nuclease digestion. the nucleic acid of purified virus was resolved by sedimentation analysis into a fast-sedimenting genome component, which comprises about two-thirds of the virion rna, and a slow-sedimenting rna, which is probably comprised of host-derived trna and a trace amount of 5 ... | 1977 | 199735 |
| eia research. | 1977 | 189175 | |
| demonstration of equine infectious anemia virus in primary leukocyte cultures by electron microscopy. | electron microscopy was used to demonstrate the presence of viral particles in primary cultures of leukocytes taken from a horse after sc inoculation with the wyoming strain of equine infectious anemia virus. unlike previous studies, the exposure virus was not passaged through cell culture prior to horse inoculation. cultures were begun approximately 1 week before and 1 week after the 1st pyrexic period after inoculation. in both samples, viral particles and cytoplasmic alterations were observed ... | 1977 | 202180 |
| electron microscopic studies on equine infectious anemia virus (eiav). brief report. | morphological studies of eiav reveal knobs on the surface of the particles, conically and tubularly shaped cores, budding particles with dense crescents directly underlying the plasma membrane, and distinct intracytoplasmic structures in infected cells. | 1977 | 202230 |
| viral antigen production in horse kidney cell cultures infected persistently with equine infectious anemia virus. | 1976 | 177893 | |
| recrudescence of equine infectious anemia by treatment with immunosuppressive drugs. | horses which had passed a few months to a few years asymptomatically after the last recurrence of equine infectious anemia (eia) showed a typical febrile response after treatment with the immunosuppressive agent, dexamethasone (dm) or cyclophosphamide (cy). in horses showing a febrile response, eia virus which had not been neutralized by neutralizing antibody previously produced was propagated. in dm-treated horses it disappeared from the blood soon after pyretolysis and antibody against the vir ... | 1976 | 177894 |
| apparent propagation of the equine infectious anemia virus in a mosquito (culex pipiens quinquefasciatus say) ovarian cell line. | a tissue culture of culex pipiens quinquefasciatus say ovarian cells appeared to support the growth of equine infectious anemia (eia) virus. shetland ponies inoculated with 2nd, 7th, 9th, and 11th passages of mediums harvested from infected tissue culture had clinical signs of the disease and became eia positive on 11, 19, 23, and 43 days after inoculation, respectively. | 1976 | 183574 |
| equine infectious anaemia. | 1976 | 181892 | |
| equine infectious anemia virus: evidence favoring classification as a retravirus. | equine infectious anemia virus (eiav) has a density of 1.154 g/cm3 in sucrose a high-molecular-weight rna similar in size to rauscher murine leukemia virus, and an internal virion reverse transcriptase that utilizes the synthetic rna template poly(ra) but not the synthetic dna template poly(da), both with (dt)12 as primer. although capable of utilizing manganese at low concentrations (approximately 0.1 mm), eiav reverse transcriptase showed highest activity in the presence of 9 mm magnesium. the ... | 1976 | 61283 |
| hemagglutination by equine infectious anemia virus. | equine infectious anemia (eia) virus which was propagated on an equine dermal cell line agglutinated guinea pig erythrocytes. viral fluids containing about 10(7.5) mean tissue culture infective doses/ml showed hemagglutinating (ha) titers ranging from 16 to 32 units/0.05 ml. results of cesium chloride equilibrium density gradient centrifugation revealed that the hemagglutinin was inseparable from the virus particles. the hemagglutination reaction persisted over a wide range of temperature and ph ... | 1976 | 9361 |
| purification and characterization of equine infectious anemia virus. | eia virus was purified from equine fetal kidney cell cultures by peg-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. purified eia virus had a density (20 degrees c) of 1.162 and a sedimentation constant of s20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. the dense core may be at various locations inside t ... | 1976 | 183628 |
| transmission of equine infectious anemia virus by tabanus fuscicostatus. | the mechanical transmission of equine infectious anemia (eia) virus by tabanus fuscicostatus was investigated. in 1 of 7 transmission trials, a single horsefly transmitted eia virus from an acutely infected pony to a susceptible pony. groups of horseflies isolated for 3, 10, or 30 minutes before refeeding transmitted eia virus, whereas those isolated for 4 or 24 hours did not. data from field studies indicate that the home range or flight distance of horseflies may exceed 4 miles. that informati ... | 1976 | 942712 |
| mechanism of viral persistence in equine infectious anemia. | 1975 | 165036 | |
| [purification and study of equine infectious anemia virus antigens, produced in vivo or in cultured cells]. | the g and c antigens of equine infectious anemia virus have been produced in vivo and in infected cell culture, then they have been purified. their physico-chemical properties have been determined, particularly their molecular weight, their sedimentation coefficient, their density and the iso electric point. | 1975 | 174831 |