| complement fixation test of equine infectious anemia. ii. relationship between cf antibody response and the disease. | | 1966 | 4292232 |
| detection of equine infectious anemia virus in vitro by immunofluorescence. | | 1971 | 4330258 |
| serial passages of equine infectious anemia virus in horse leukocyte culture. | | 1967 | 4293211 |
| complement-mediated hemolysis of horse erythrocytes treated with equine infectious anemia virus. | horse erythrocytes treated with equine infectious anemia virus hemagglutinin were found to be lysed after incubation with fresh horse serum at 37 degrees c. fresh guinea pig serum induced more efficient hemolysis than horse serum. direct immunofluorescence test revealed the adsorption of complement factors on the surface of the erythrocytes. calcium and magnesium ions were necessary for the hemolysis to take place. antibody against equine infectious anemia virus enhanced the virus-induced comple ... | 1987 | 3036045 |
| phagocytosis of horse erythrocytes treated with equine infectious anemia virus by cultivated horse leukocytes. | horse erythrocytes treated with equine infectious anemia virus hemagglutinin were phagocytized by cultivated horse leukocytes (mainly macrophage-like cells and partly polymorphonuclear cells) after incubation with fresh horse serum but not with inactivated horse serum. the phagocytosis began as soon as the erythrocytes were added to the leukocyte cultures, and the majority of the reaction proceeded within 30 minutes. addition of antiserum showed a slightly suppressing but no enhancing effect on ... | 1987 | 3036046 |
| viremia and immunological responses in horses infected with equine infectious anemia virus. | | 1969 | 4306393 |
| attempts to cultivate the equine infectious anemia virus in various types of cells. | | 1968 | 4307837 |
| growth characteristics of equine infectious anemia virus in horse leukocyte cultures. brief report. | | 1970 | 4318580 |
| growth of the equine infectious anemia virus in a continuous-passage horse leukocyte culture. | | 1970 | 4319261 |
| [animal viral anemia: equine infectious anemia]. | | 1970 | 4320458 |
| changes in pathogenicity of equine infectious anemia virus during passages in horse leukocyte cultures. | | 1970 | 4320651 |
| immunization of horses against equine infectious anemia (eia) with an attenuated eia virus. | | 1970 | 4320652 |
| physicochemical studies of equine infectious anemia virus. iv. determination of the nucleic acid type in the virus. | | 1970 | 4321449 |
| growth of equine infectious anemia in a new cell system. | | 1969 | 4323215 |
| molecular cloning and physical characterization of integrated equine infectious anemia virus: molecular and immunologic evidence of its close relationship to ovine and caprine lentiviruses. | molecular clones of the integrated form of the genome of equine infectious anemia virus (eiav), the etiologic agent of a naturally occurring, worldwide disease of horses, were obtained. the restriction map of a full-length genome was determined. additional evidence for the close evolutionary relationship between eiav and a prototype lentivirus (caprine arthritis encephalitis virus) was acquired by southern blotting and immunological analyses. an interspecies radioimmunoassay was developed in whi ... | 1986 | 3750842 |
| distribution of equine infectious anemia virus in horses infected with the virus. | | 1971 | 4325551 |
| comparison of glycoproteins by two-dimensional mapping of glycosylated peptides. | we describe here a two-dimensional mapping procedure which is capable of resolving glycopeptides isolated by lectin affinity chromatography from radioiodinated tryptic digests of glycoproteins. glycopeptide maps were successfully produced for the model proteins alpha 1-acid glycoprotein and fetuin, as well as for the two surface glycoproteins gp90 and gp45 from equine infectious anemia virus (eiav). differences were detected in the glycopeptide maps obtained for the gp90 and gp45 components from ... | 1986 | 3021020 |
| immunopathology of equine infectious anemia. | | 1971 | 4328671 |
| enzymes of equine erythrocytes: changes during equine infectious anemia. | | 1969 | 4304330 |
| [recent virological and immunological findings in infectious anemia in horses]. | | 1971 | 4329836 |
| equine infectious anemia: preliminary investigation of the complement-fixation test for the demonstration of antibodies and antigen. | clinical field cases of equine infectious anemia were studied and the disease was reproduced experimentally in horses. attempts were made to adapt the complement-fixation test to the detection of antibodies in the serum of infected animals and to the demonstration of antigens in tissue extracts.a moderate complement-fixing antibody response was demonstrated in the serum of horses shortly after primary exposure to the infectious agent. however, this reactivity was of short duration and occurred w ... | 1969 | 4305760 |
| characteristics of the complement-fixing antigen of equine infectious anemia virus. | | 1968 | 4305805 |
| serological comparison among various strains of equine infectious anemia virus. | | 1971 | 4330134 |
| titration of equine infectious anemia virus. effect of dosage on incubation time and clinical signs. | | 1971 | 4330637 |
| nucleotide sequence analysis of equine infectious anemia virus proviral dna. | the nucleotide sequence of the integrated form of the genome of the equine infectious anemia virus was determined. by comparison with ltr sequences of other retroviruses, signals for the control of viral gene transcription and translation could be identified in the eiav ltr. open reading frames for gag and pol genes were identified and their sequences matched very closely to those determined previously by others. however, in the present study, the pol gene reading frame was open throughout its e ... | 1987 | 3035786 |
| quantitation of immunoglobulin-bearing lymphocytes and lymphocyte response to mitogens in horses persistently infected by equine infectious anemia virus. | a defect in lymphocyte function could be responsible for persistent infection by the equine infectious anemia virus. the number of lymphocytes bearing surface immunoglobulin, as detected by immunofluorescence, and lymphocyte response to mitogens were the same in uninfected and equine infectious anemia-infected animals. a defect in t or b lymphocyte numbers or ability to respond to stimuli was not detected in this chronic virus disease. | 1973 | 4582640 |
| [outbreak of infectious anemia among horses on a farm--an epidemiological experiment]. | | 1967 | 4306358 |
| physicochemical studies of equine infectious anemia virus. i. buoyant density of the virus. | | 1969 | 4306374 |
| physicochemical studies of equine infectious anemia virus. ii. sensitivity of the virus to trypsin. | | 1969 | 4306375 |
| detection of precipitating antibody in equine infectious anemia by concentrated virus antigen. | | 1972 | 4340682 |
| [cultivation of equine infectious anemia virus]. | | 1970 | 4325782 |
| extraction of equine infectious anemia immunodiffusion antigen with the aid of the chaotropic agent, thiocyanate. | immunodiffusion antigen from spleens of horses infected with equine infectious anemia virus was prepared by methods employing freeze-thaw cycles and thiocyanate treatment. thiocyanate (0.5 m) permitted the recovery of the greatest amount of antigen. furthermore, it was most effective for recovery of immunodiffusion antigen from spleens which yielded unsatisfactory concentrations of antigen by the conventional freeze-thaw or water-extraction methods. the reactivity of the antigen did not appear t ... | 1973 | 4348466 |
| physicochemical studies of equine infectious anemia virus. v. effect of ultraviolet irradiation on virus infectivity. | | 1973 | 4352062 |
| passive immunity in the foal: measurement of immunoglobulin classes and specific antibody. | | 1973 | 4355952 |
| [equine infectious anemia--recent researches and prospect of the study (author's transl)]. | | 1973 | 4356439 |
| diagnosis of equine infectious anemia by immunodiffusion test. | | 1972 | 4333633 |
| preparation of equine infectious anemia virus antigen for immunodiffusion test. | | 1973 | 4358258 |
| production of equine infectious anemia antigen in a persistently infected cell line. | | 1973 | 4358259 |
| equine infectious anemia: a retrospective study of an epizootic. | | 1974 | 4358831 |
| equine infectious anemia. | | 1974 | 4359480 |
| [indication of virus of equine infectious anemia]. | | 1970 | 4324050 |
| excretion of equine infectious anemia virus from horses infected with the virus. | | 1973 | 4366013 |
| spontaneous autoimmune diseases of domestic animals. | | 1974 | 4367527 |
| equine infectious anemia. | | 1974 | 4371283 |
| equine infectious anemia virus from infected horse serum. | equine infectious anemia virus was purified from infected horse serum samples. electron microscope observation on negatively stained preparations of purified virus showed roughly spherical particles sized between 100 and 200 nm in diameter. in disrupted particles, an envelope was visible but no internal structure could be resolved. since the purified virus fraction had a strong antigenic activity to antiserum in immunodiffusion reaction, these particles are thought to be the causative virus of e ... | 1974 | 4372175 |
| studies on complement-fixation reaction in equine infectious anemia. i. development and activities of complement-fixing and complement fixation-inhibiting antibodies. | | 1974 | 4374566 |
| role of horse fly (tabanus fuscicostatus hine) and stable fly (stomoxys calcitrans l.) in transmission of equine infectious anemia to ponies in louisiana. | | 1973 | 4357708 |
| prevalence of antibodies to herpesvirus types 1 and 2, arteritis and infectious anemia viral antigens in equine serum. | | 1974 | 4360338 |
| demonstration of antigenic identity between purified equine infectious anemia virus and an antigen extracted from infected horse spleen. | antigenic relationship between purified equine infectious anemia (eia) virus and spleen-derived antigen from eia-infected horses was examined by immunodiffusion. identical antigenicity of these two antigens has been proven because precipitation lines formed between the two antigens and eia antiserum connected with each other. the results indicate that the antigenic substance derived from infected spleen is a component of eia virus. | 1972 | 4629262 |
| immunologically mediated glomerulitis of horses. i. pathogenesis in persistent infection by equine infectious anemia virus. | | 1972 | 4337973 |
| study of the one-step growth curve of equine infectious anemia virus by immunofluorescence. | primary horse leukocyte cultures were inoculated with 2 or 10 50% tissue culture infective doses (tcid(50)) of equine infectious anemia (eia) virus per cell, and the titer of cell-associated and fluid-phase virus was determined from 1 to 72 hr postinoculation (pi). cover slips were collected from 4 to 72 hr pi and stained for eia viral antigen by the indirect immunofluorescent (fa) technique. viral replication was detected after a latent period of approximately 18 to 24 hr and reached peak titer ... | 1972 | 4344094 |
| enzyme-linked immunosorbent assay for detection of equine infectious anemia antibody to purified p26 viral protein. | an indirect enzyme-linked immunosorbent assay (elisa) was developed for the detection of equine infectious anemia (eia) antibody in horse sera. purified p26 viral protein was the antigen; alkaline phosphatase linked to rabbit anti-horse immunoglobulin g was the conjugate. the elisa detected eia antibodies in horse sera as early as 11 to 14 days after experimental inoculations. there was full agreement between the results of elisa and the agar-gel immunodiffusion tests on eia proficiency test ser ... | 1984 | 6089620 |
| unsuccessful attempts to propagate equine infectious anemia virus in lambs and small experimental animals. | | 1974 | 4375794 |
| filtrability of equine infectious anemia virus. | | 1972 | 4339550 |
| propagation of equine infectious anemia virus in horse kidney cell cultures. | | 1974 | 4375791 |
| the role of stable flies and mosquitoes in the transmission of equine infectious anemia virus. | | 1980 | 6118874 |
| studies with equine infectious anemia virus: transmission attempts by mosquitoes and survival of virus on vector mouthparts and hypodermic needles, and in mosquito tissue culture. | biological and mechanical transmission trials with psorophora columbiae (dyar and knab) and aedes sollicitans (walker) and ponies acutely infected with equine infectious anemia virus (eiav) were negative. the eiav antigen was detected by radioimmunoassay in ae sollicitans immediately after the mosquitoes had fed on an acutely ill pony, but not 14 days after feeding. psorophora columbiae mosquitoes had detectable eiav antigen as determined by radioimmunoassay 24 hours after they fed on an acutely ... | 1981 | 6119953 |
| antigenic reactivity of the major glycoprotein of equine infectious anemia virus, a retrovirus. | the immunogenic contributions of the carbohydrate and peptide portions of the major envelope glycoprotein of equine infections anemia virus, eiav gp90, were analyzed by measuring the effects of specific glycosidase and protease digestions on the reactivity of the glycoprotein with immune sera from infected horses. the results of both direct and competitive radioimmunoassay demonstrated that immune sera contained antibodies reactive with both the carbohydrate and protein moieties of eiav gp90, wi ... | 1984 | 6205503 |
| antigenic variation during persistent infection by equine infectious anemia virus, a retrovirus. | the recurrent nature of equine infectious anemia has been attributed to relatively rapid antigenic variations in equine infectious anemia virus (eiav) during persistent infection under selective immune pressures. this model was tested by serological and biochemical analysis of virus isolates recovered from separate febrile episodes in two experimentally infected ponies. neutralization assays employing immune sera from the experimentally infected ponies demonstrated that distinct antigenic strain ... | 1984 | 6206055 |
| isolation and characterization of low-molecular-weight dna-binding proteins from retroviruses. | | 1980 | 6249039 |
| hemagglutination-inhibition tests with different strains of equine infectious anemia virus. | the serologic relationships between 6 strains of equine infectious anemia (eia) viruses were investigated by hemagglutination-inhibition (hi) tests. cross hi tests, using sera from horses in the early stage of infection, revealed that all strains were inhibited only by homologous strain antisera and that hi antibody was always detectable before virus-neutralizing antibody. in the later stages of infection, both homologous and heterologous hi antibodies were detected in a sera of most of the hors ... | 1981 | 6175255 |
| isolation and comparative biochemical properties of the major internal polypeptides of equine infectious anemia virus. | we describe procedures for the large-scale production of equine infectious anemia virus (eiav) and for the isolation of the four major non-glycosylated virion proteins, designated p26, p15, p11, and p9. comparisons of the purified proteins by peptide mapping procedures and by enzyme-linked immunosorbent assays demonstrated the unrelatedness of the four proteins. the characteristic properties of each purified protein were examined by determining isoelectric points and amino acid compositions. we ... | 1982 | 6178843 |
| [comparative investigations on the serological diagnosis of the equine infectious anemia (author's transl)]. | | 1981 | 6262037 |
| hemagglutination of several strains of equine infectious anemia virus. | six strains of equine infectious anemia (eia) virus propagated in equine leukocyte cultures were found to agglutinate horse erythrocytes. concentrated virus material containing about 20 units of complement fixation (cf) titer showed hemagglutinating (ha) titers ranging from 4 to 8 units. the ha activity remained stable after ether treatment and was reduced by trypsin, formaldehyde and kio4. cesium chloride equilibrium density gradient centrifugation revealed two populations of hemagglutinin, one ... | 1981 | 6263225 |
| transmission of equine infectious anaemia virus from a horse negative to agar gel immunodiffusion testing. | | 1981 | 6265206 |
| the immune system in slow disease due to persistent submicrobial (viral) infection. | | 1981 | 6270625 |
| propagation of equine infectious anemia virus in horse cell cultures. | | 1981 | 6272480 |
| high-performance gel permeation chromatography of proteins in denaturing solvents and its application to the analysis of enveloped virus polypeptides. | | 1981 | 6272599 |
| prevalence of equine infectious anaemia (swamp fever) in guyana. | | 1981 | 6272928 |
| in vitro host range of equine infectious anemia virus. | equine infectious anemia virus (eiav) was successfully inoculated onto cell cultures of canine and feline origin, resulting in chronic infections in these cultures. infection of equine cell cultures, which were the previous sole in vitro source demonstrated for virus production, was also performed for comparative purposes. determination of the nature of the virus produced in the heterologous as well as the equine cells was accomplished in several ways. sds-page of purified virus from the differe ... | 1981 | 6177659 |
| equine infectious anemia virus, a putative lentivirus, contains polypeptides analogous to prototype-c oncornaviruses. | | 1980 | 6256947 |
| orientation of structural polypeptides of equine infectious anemia virus. | lactoperoxidase iodination of intact and disrupted equine infectious anemia virus revealed that glycopeptide gp79 is the major surface component of this virus, whereas glycopeptides gp64 and gp40 as well as the principle nonglycosylated structural polypeptides p29 and p13 are internal. virus 'envelope' particles, banding in isopycnic centrifugation at approximately 1.10 g/cm3, contained the glycopeptides but no internal nonglycosylated proteins. glycopeptides gp79, gp64 and gp40 and core polypep ... | 1980 | 6259082 |
| resistance of horses infected chronically with equine infectious anemia virus against reinfection. | | 1973 | 4792269 |
| [studies on tissue culture of equine infectious anemia virus. vii. evaluation of bovine serum used for equine leukocyte culture with special reference to the relationship between the serum protein fraction pattern and the culture growth]. | | 1966 | 4960853 |
| genomic alterations associated with persistent infections by equine infectious anaemia virus, a retrovirus. | the unique periodic nature of equine infectious anaemia (eia) is believed to result from the ability of the infecting virus. eiav, to undergo relatively rapid antigenic variations which circumvent host immune responses resulting in distinct virus populations in sequential clinical episodes in the persistently infected horse. this model was examined by oligonucleotide mapping comparisons of the rna genomes of selected isolates of eiav. variations in oligonucleotide maps could be reproducibly demo ... | 1984 | 6086822 |
| antigenic stimulation of t lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia. | equine infectious anemia is a chronic disease of horses caused by a nononcogenic retrovirus. studies were undertaken to determine the types of cells involved in the in vitro lymphoproliferative response to viral antigens and the dynamics of this reaction. it was observed that reactive lymphocytes were present at unpredictable times in the peripheral blood of infected horses. this reaction was shown to be specific for the interaction of equine infectious anemia virus and t lymphocytes. enriched b ... | 1982 | 6281191 |
| persistent infection by equine infectious anemia virus: asymmetry of nucleotide sequence reiteration in the integrated provirus of persistently infected cells. | | 1982 | 6281971 |
| dna sequence relationship of the baboon endogenous virus genome to the genomes of other type c and type d retroviruses. | baboon endogenous virus (baev) is a type c retrovirus present in multiple proviral copies in the dna of baboons. although interspecies antigenic determinants present on reverse transcriptase and gag proteins are shared among all mammalian type c viruses, no nucleic acid homology between baev and other type c viruses (except rd-114) has been found in conventional liquid hybridization experiments. in this study, we used restriction fragments of cloned baev dna immobilized on nitrocellulose to test ... | 1982 | 6284972 |
| virulence and in vitro growth of a cell-adapted strain of equine infectious anemia virus after serial passage in ponies. | five serial passages of a cell-adapted strain of equine infectious anemia (eia) virus were conducted in shetland ponies. the 13 recipient ponies became agar-gel immunodiffusion test-positive by 25 days after they were inoculated. the virulence of the cell-adapted strain of eia virus markedly increased through 3 serial passages, although individual variation within passages was high. the 1st serial-passage recipient remained afebrile through 200 days, whereas a febrile episode occurred about ever ... | 1982 | 6293349 |
| detection of equine infectious anemia virus in a horse with an equivocal agar gel immunodiffusion test reaction. | a horse whose serum reacted equivocally in the agar gel immunodiffusion (agid) test for equine infectious anemia was studied over a 3-year period. the horse remained afebrile and virus was detected in only 1 of 6 horse inoculation tests. the intensity of agid test reactions increased temporarily following this evidence for virus. although the agid test reaction was equivocal and 5 of the 6 transmission attempts failed, the 1 successful transmission proved the horse was infected. | 1982 | 6276354 |
| serological survey for equine infectious anaemia. | | 1981 | 6275830 |
| transmission and clinical evaluation of an equine infectious anemia herd and their offspring over a 13-year period. | | 1984 | 6321418 |
| an overview of equine infectious anemia control and regulation in the united states. | | 1984 | 6321419 |
| studies on equine infectious anemia virus transmission by insects. | there are several factors involved in the mechanical transmission of equine infectious anemia (eia) virus by insects. large hematophagous insects, especially tabanids, which feed from extravascular sites (ie, pool feeding) appear to be the most efficient vectors. the biology of the host-seeking and blood-feeding behavior of the vectors are important variables that have been overlooked in the mechanical transmission of pathogens like eia virus. the biology, population levels, and diversity of the ... | 1984 | 6321420 |
| detection of equine infectious anemia virus in horse leukocyte cultures derived from horses in various stages of equine infectious anemia viral infection. | the enzyme-linked immunosorbent assay (elisa) antigen-positive and agar-gel immunodiffusion test (agid)-negative horses do not have infective equine infectious anemia (eia) virus. the elisa testing of horse leukocyte culture (hlc) supernatants did detect eia virus in a hlc that was infected with the wyoming strain of eia virus and in hlc derived from horses in febrile, acute, or subacute stages of eia infection. in supernatants of hlc derived from chronic and inapparent carrier horses, eia virus ... | 1984 | 6322623 |
| structural proteins of equine infectious anemia virus and their antigenic activity. | using purified equine infectious anemia (eia) virus labeled with 3h-glucosamine or 14c-protein hydrolysate, structural proteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. as a result, 2 glycoproteins and 10 proteins with molecular weights (mol wt) ranging from 12,000 to 115,000 daltons were demonstrated. of 12 structural proteins, 3 proteins, namely a glycoprotein with mol wt of 76,000 (gp76) and 2 proteins with mol wt of 25,000 (p25) and 12,000 (p12), respective ... | 1984 | 6322625 |
| transmission of equine infectious anemia virus from horses without clinical signs of disease. | twenty seven adult horses positive to the agar gel immunodiffusion (agid) test for equine infectious anemia (eia), but with no history of clinical eia, were used in transfusion studies to determine whether infectious eia virus was present in 1 to 5 ml of their blood. of 27 recipients, 21 (78%) became agid test-positive at an average of 24 days after inoculation. two horses that were initially negative when screened were retested and found to carry infectious virus in 5-300 ml of whole blood; the ... | 1982 | 6276353 |
| isolation of equine infectious anemia virus glycoproteins. lectin affinity chromatography procedures for high avidity glycoproteins. | lectin affinity chromatography procedures were evaluated for the isolation of enveloped virus glycoproteins. the major glycoprotein of equine infectious anemia virus (eiav) bound to concanavalin a (con a)-sepharose through interactions which could not be reversed by alpha-methylglucoside, but elution could be accomplished with buffers containing guanidine hydrochloride or sodium dodecyl sulfate. these denaturants, however, also released about one-half of the con a protein from the sepharose matr ... | 1983 | 6309879 |
| propagation of equine infectious anemia virus in horse cell cultures. | the wyoming strain of equine infectious anemia virus was adapted to cell cultures by 7 passages in horse leukocytes and 14 passages in fetal equine dermal and kidney cells. the virus was made evident by electron microscopy and immunodiffusion tests with antigens prepared from culture fluids. | 1981 | 6306910 |
| carriers of equine infectious anemia virus. | presently available data continue to support the idea that once a horse is infected with equine infectious anemia virus it remains infected indefinitely. infection may not always be demonstrated by inoculation of plasma, serum, or whole blood transfusions into susceptible recipients, but transfusions of fresh whole blood will be infective in at least 95% of the horses testing positive in the agar gel immunodiffusion test. for detection of infectivity in a small percentage of inapparent carriers, ... | 1984 | 6421787 |
| cytoplasmic inclusions in cells infected with the virus of equine infectious anemia (eiav). | after infection with equine infectious anemia virus (eiav) equine dermal (ed) cells revealed fluorescent spots in their cytoplasm which were detected with an indirect immunofluorescence technique. to characterize the nature of these cytoplasmic inclusions, comparative light and electron microscopic studies were performed. the cytoplasmic location of the spots observed in the indirect immunofluorescence assay coincided with the location of structures detected with the electron microscope. in ultr ... | 1984 | 6325195 |
| equine infectious anemia virus tat is a predominantly helical protein. | nuclear magnetic resonance (nmr) spectroscopy revealed features of the secondary structure of the equine infectious anemia virus (eiav) tat protein in solution. we could show that this protein, which is required in the replication cycle of lentiviruses, forms a predominantly helical structure in trifluoroethanol/water (40% by vol.) solution. in particular, the basic rna-binding region and the adjacent core domain, which are highly conserved among lentiviral tat proteins, show helix-type secondar ... | 1993 | 7506657 |
| indirect hemagglutination test in equine infectious anemia. | an indirect hemagglutination was developed for the diagnosis of equine infectious anemia using sheep red blood cells coated with group specific virus antigen which had been highly purified by affinity chromatography. the presence of indirect hemagglutination antibodies was demonstrated in horses with equine infectious anemia since the cells were specifically agglutinated by all the serum samples obtained from experimentally infected horses. antibodies appeared within 35 days after inoculation, a ... | 1982 | 6280821 |
| the aspartic proteinase of equine infectious anaemia virus. | | 1994 | 7821563 |
| enzyme-linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody. | a sensitive specific enzyme-linked immunosorbent assay utilizing purified p26 antigen was developed for the detection of antibodies to equine infectious anemia virus in naturally and experimentally infected horses. generally, antibodies to the virus could be detected by the enzyme-linked immunosorbent assay 3 to 4 days earlier than by the standard agar gel immunodiffusion test, and they could be detected more reliably in horses with weak or equivocal agar gel immunodiffusion test reactions. the ... | 1984 | 6325488 |
| screening anti-hiv chinese materia medica with hiv and equine infectious anemic virus reverse transcriptase. | the inhibitory activities of human immunodeficiency virus reverse transcriptase (hiv-rt) inhibitors pfa and suramin against hiv-rt and equine infectious anemic virus reverse transcriptase (eiav-rt) were studied in this paper. the 50% inhibitory concentration (ic50) of hiv-rt and eiav-rt treated by pfa and suramin were 0.2 mumol, 9.8 mumol and 17 mumol, 19.9 mumol, respectively. more than thirty chinese medicines, including recipes, herbs, extracts of traditional materia medica and isolated compo ... | 1994 | 7515133 |
| characterization of monoclonal antibodies directed against the envelope proteins of feline leukemia virus. | monoclonal antibodies directed against the feline leukemia virus (felv) envelope proteins, gp70 and p15e, were identified by radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. six of these monoclonal antibodies were specific for the gp70; two for the p15e. enzyme-linked immunosorbent assay binding assays against felv subtypes a, b, and c showed that most of the monoclonal antibodies bound to more than one subtype but have a greater affinity for subtype b. one ... | 1984 | 6331649 |
| envelope sequence variation, neutralizing antibodies, and primate lentivirus persistence. | studies in ungulate lentivirus systems clearly indicate that neutralization escape variants emerge over time in chronically infected animals. studies in the eiav system, in particular, have provided strong evidence that the humoral branch of the immune system is at least one selective force acting on an array of viral variants. in previous studies with the ungulate lentiviruses, molecularly cloned virus was never used, and plaque-purified virus was only sometimes used; the genetic determinants r ... | 1994 | 7523031 |
| nmr analysis of the trans-activation response (tar) rna element of equine infectious anemia virus. | transcription of lentiviral dna in the host cell is regulated by an interaction between the viral tar rna stem-loop and the viral tat protein. here we present a model of the three-dimensional structure of the tar rna stem-loop of the equine infectious anemia virus (eiav), derived from two- and three-dimensional nmr data. this 25 nucleotide rna consists of an a-form helical stem capped by two u-g base pairs and a four-nucleotide loop. two loop cytidines are stacked into the loop interior and like ... | 1995 | 7479065 |
| lentivirus cross-reactive determinants present in the capsid protein of equine infectious anaemia virus. | in this study we used immune sera from equine infectious anaemia virus (eiav)-infected horses which uniquely display broad reactivity with different lentivirus capsid proteins (ca) to characterize the cross-reactive determinants of lentivirus ca proteins. in particular, the role of the major homology region (mhr) of lentivirus ca proteins in this serological cross-reactivity was evaluated using both equine immune serum and murine monoclonal antibodies (mabs) directed against the mhr segment of d ... | 1994 | 7510329 |