Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| immunoprecipitation of a 50-kda protein: a candidate receptor component for tomato spotted wilt tospovirus (bunyaviridae) in its main vector, frankliniella occidentalis. | a 50-kda protein that binds to viral particles in solid-phase assays and that is recognized by anti-idiotypic antibodies made against anti-viral glycoproteins g1/g2 (anti-ids) has been proposed as a receptor candidate for tomato spotted wilt tospovirus (tswv) in its main thrips vector, frankliniella occidentalis pergande (bandla et al., 1998. phytopathology 88, 98-104). here we show the immunoprecipitation of the 50-kda protein by anti-ids and by an anti-g1/g2-tswv conjugate - a new immunoprecip ... | 2000 | 10867190 |
| real-time rt-pcr fluorescent detection of tomato spotted wilt virus. | a real-time reverse transcription-polymerase chain reaction assay based on taqman chemistry was developed for the detection and quantification of tomato spotted wilt virus (tswv). this method enabled sensitive, reproducible and specific detection of tswv in 'leaf soak' and total rna extracts from infected plants. taqman reliably detected tswv in as little as 500 fg total rna. the assay was 10-fold more sensitive than visualisation of ethidium bromide-stained bands following agarose gel electroph ... | 2000 | 10921836 |
| comparative genetics of disease resistance within the solanaceae. | genomic positions of phenotypically defined disease resistance genes (r genes) and r gene homologues were analyzed in three solanaceous crop genera, lycopersicon (tomato), solanum (potato), and capsicum (pepper). r genes occurred at corresponding positions in two or more genomes more frequently than expected by chance; however, in only two cases, both involving phytophthora spp., did genes at corresponding positions have specificity for closely related pathogen taxa. in contrast, resistances to ... | 2000 | 10835406 |
| genetic mapping of the tsw locus for resistance to the tospovirus tomato spotted wilt virus in capsicum spp. and its relationship to the sw-5 gene for resistance to the same pathogen in tomato. | the tsw gene conferring dominant resistance to the tospovirus tomato spotted wilt virus (tswv) in capsicum spp. has been tagged with a random amplified polymorphic dna marker and mapped to the distal portion of chromosome 10. no mapped homologues of sw-5, a phenotypically similar dominant tswv resistance gene in tomato, map to this region in c. annuum, although a number of sw-5 homologues are found at corresponding positions in pepper and tomato. the relationship between tsw and sw-5 was also ex ... | 2000 | 10830267 |
| simplified, rapid method for cloning of virus-binding polypeptides (putative receptors) via the far-western screening of a cdna expression library using purified virus particles. | a simplified, alternative method for cloning virus-binding polypeptides (receptor candidates) is described. the method is based on a far-western assay using purified tomato spotted wilt tospovirus (tswv, bunyaviridae) for screening a lambda-phage cdna expression library. the western flower thrips, frankliniella occidentalis pergande, the principal vector of tswv, in which the virus replicates, was used for library construction. using this method several virus-binding polypeptides were identified ... | 2000 | 10785290 |
| a minimum length of n gene sequence in transgenic plants is required for rna-mediated tospovirus resistance. | we showed previously that transgenic plants with the green fluorescent protein (gfp) gene fused to segments of the nucleocapsid (n) gene of tomato spotted wilt virus (tswv) displayed post-transcriptional gene silencing of the gfp and n gene segments and resistance to tswv. these results suggested that a chimeric transgene composed of viral gene segments might confer multiple virus resistance in transgenic plants. to test this hypothesis and to determine the minimum length of the n gene that coul ... | 2000 | 10640563 |
| a caps marker to assist selection of tomato spotted wilt virus (tswv) resistance in pepper. | the hypersensitive resistance to tomato spotted wilt virus (tswv) in pepper is determined by a single dominant gene (resistant allele: tsw) in several capsicum chinense genotypes. in order to facilitate the selection for this resistance, four rapd (among 250 10-mer primers tested) were found linked to the tsw locus using the bulked segregant analysis and 153 f2 individuals. a close rapd marker was converted into a codominant cleaved amplified polymorphic sequence (caps) using specific pcr primer ... | 2000 | 10701122 |
| tomato spotted wilt virus glycoproteins exhibit trafficking and localization signals that are functional in mammalian cells. | the glycoprotein precursor (g1/g2) gene of tomato spotted wilt virus (tswv) was expressed in bhk cells using the semliki forest virus expression system. the results reveal that in this cell system, the precursor is efficiently cleaved and the resulting g1 and g2 glycoproteins are transported from the endoplasmic reticulum (er) to the golgi complex, where they are retained, a process that could be blocked by tunicamycin. expression of g2 alone resulted in transport to and retention in the golgi c ... | 2001 | 11134314 |
| gst fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies. | glutathione s-transferase (gst) fusion proteins are used frequently for investigating protein-protein and protein-dna interactions. the present study demonstrates that the use of gst fusion proteins caused false positives during selection of phage-displayed single-chain antibody fragments (scfvs) specific for three domains of the movement protein (ns(m)) of tomato spotted wilt virus (tswv). to identify and exclude the false positives when using gst as a fusion partner linked to the antigen of in ... | 2001 | 11164495 |
| variability of the n-protein and the intergenic region of the s rna of tomato spotted wilt tospovirus (tswv). | the genetic heterogeneity of the n protein gene and the intergenic region (igr) of the s rna from tomato spotted wilt virus (tswv) isolates, collected in bulgaria, were compared with isolates from other parts of the world. the results substantiated the highly conserved nature of the n protein. twenty six independent sequences revealed only seven variable amino acid positions, common to all isolates. the type of amino acids present in these positions seems to be independent of the geographical or ... | 2001 | 11346302 |
| visual representation by atomic force microscopy (afm) of tomato spotted wilt virus ribonucleoproteins. | atomic force microscopy (afm) allows the observation of biological material without fixation procedures. here we present afm images of ribonucleoproteins (nucleocapsids) derived from a plant infecting rna virus (tomato spotted wilt virus, tswv), which have been recorded in contact mode. the nucleocapsids, prepared from systemically infected leaves of tobacco, were spreaded on a glass surface and dried in air, and appeared as regularly formed rings, resembling the proposed pseudocircular and panh ... | 2001 | 11767945 |
| overcoming host- and pathogen-mediated resistance in tomato and tobacco maps to the m rna of tomato spotted wilt virus. | a viral genetic system was used to map the determinants of the ability of tomato spotted wilt virus (tswv) to overcome the r gene (sw-5) in tomato and the resistance conferred by the nucleocapsid gene of tswv (n gene) in tobacco. a complete set of reassortant genotypes was generated from tswv isolates a and d. tswv-a was able to overcome the sw-5 gene in tomato and the tswv n gene in tobacco, whereas tswv-d was repressed by both forms of resistance. the ability to overcome both forms of resistan ... | 2001 | 11204788 |
| in vivo analysis of the tswv cap-snatching mechanism: single base complementarity and primer length requirements. | requirements for capped leader sequences for use during transcription initiation by tomato spotted wilt virus (tswv) were tested using mutant alfalfa mosaic virus (amv) rnas as specific cap donors in transgenic nicotiana tabacum plants expressing the amv replicase proteins. using a series of amv rna3 mutants modified in either the 5'-non-translated region or in the subgenomic rna4 leader, sequence analysis revealed that cleaved leader lengths could vary between 13 and 18 nucleotides. cleavage oc ... | 2001 | 11350944 |
| rt-pcr for detecting five distinct tospovirus species using degenerate primers and dsrna template. | rt-pcr procedures for detection of multiple species of tospovirus from plant tissues were investigated. downstream primers were designated from the 3' untranslated sequences of the s rna. an upstream primer was designated from the degenerated sequences of the nucleocapsid protein. approximately 450 bp dna fragments were detected when tomato spotted wilt virus (tswv)- or impatiens necrotic spot virus (insv)- infected tissues were examined. approximately 350 bp dna fragments were detected when wat ... | 2001 | 11445145 |
| overwintering of frankliniella fusca (thysanoptera: thripidae) on winter annual weeds infected with tomato spotted wilt virus and patterns of virus movement between susceptible weed hosts. | abstract overwintering of tobacco thrips, frankliniella fusca, was investigated on common winter annual host plants infected with tomato spotted wilt virus (tswv). populations of tobacco thrips produced on tswv-infected plants did not differ from those produced on healthy plants, whereas populations varied greatly among host plant species. the mean per plant populations of f. fusca averaged 401, 162, and 10 thrips per plant on stellaria media, scleranthus annuus, and sonchus asper, respectively, ... | 2001 | 18944235 |
| serological and molecular characterization of peanut chlorotic fan-spot virus, a new species of the genus tospovirus. | abstract to clarify the serological relationship of peanut chlorotic fan-spot virus (pcfv) with other tospoviruses, antisera were produced against the nucleocapsid (n) proteins of this virus and tospoviruses from four serogroups including tomato spotted wilt virus (tswv), impatiens necrotic spot virus (insv), groundnut ringspot virus (grsv), and watermelon silver mottle virus (wsmov). in immunodiffusion tests, the antisera only reacted with their homologous antigens. similar results were noticed ... | 2001 | 18944231 |
| completion of the genome sequence of watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. | abstract the nucleotide sequence of the l rna of watermelon silver mottle virus (wsmov) was determined. combined with the previous work on m and s rnas, the whole genomic sequence of this member of the genus tospovirus was completed. the l rna is 8,917 nucleotides in length, with one large open reading frame encoding a translation product of 2,878 amino acids (331.8 kda) on the viral complementary strand. the l protein shares amino acid identities of only 44.3 and 46.5% with tomato spotted wilt ... | 2001 | 18943848 |
| replication of tomato spotted wilt virus after ingestion by adult thrips setosus is restricted to midgut epithelial cells. | abstract if acquisition access feeding (aaf) is first given after adult eclosion, none of the nine thrips species able to serve as tospovirus vectors can become infective. the previous cellular investigations of this phenomenon, carried out only in frankliniella occidentalis, suggested that infectivity was prevented because the type member of the tospoviruses, tomato spotted wilt virus (tswv), was unable to enter the midgut of adult thrips. the present study extends a cellular view of tospovirus ... | 2001 | 18943329 |
| dynamics of tomato spotted wilt virus replication in the alimentary canal of two thrips species. | abstract transmission of tomato spotted wilt virus (tswv) is dependent on virus uptake in the midgut prior to virus movement to the salivary glands. replication of tswv in the alimentary canal of tobacco thrips (tt, frankliniella fusca) and western flower thrips (wft, f. occidentalis) was investigated by immunolocalization of the nonstructural protein (nss) encoded by the small rna of tswv and fluorescence microscopy. analysis of cohorts during development from larva to adults following virus ac ... | 2002 | 18943268 |
| the efficiency by which thrips tabaci populations transmit tomato spotted wilt virus depends on their host preference and reproductive strategy. | abstract arrhenotokous and thelytokous populations of thrips tabaci from tobacco or leek plants were evaluated for their ability to transmit tomato spotted wilt virus (tswv) and for their host preference. transmission efficiencies were comparatively studied using leaf disks of petunia hybrida, datura stramonium, and nicotiana tabacum cv. basmas. adults of arrhenotokous populations collected on infected tobacco plants in the field were efficient transmitters (up to 48.5% transmission) and remaine ... | 2002 | 18944256 |
| identification of a novel rna silencing suppressor, nss protein of tomato spotted wilt virus. | rna silencing or post-transcriptional gene silencing (ptgs) in plants is known as a defense system against virus infection. several plant viruses have been shown to encode an rna silencing suppressor. using a green fluorescent protein-based transient suppression assay, we show that nss protein of tomato spotted wilt virus (tswv) has rna silencing suppressor activity. tswv nss protein suppressed sense transgene-induced ptgs but did not suppress inverted repeat transgene-induced ptgs. tswv nss pro ... | 2002 | 12459466 |
| purified tomato spotted wilt virus particles support both genome replication and transcription in vitro. | purified tomato spotted wilt virus particles were shown to support either genome replication or transcription in vitro, depending on the conditions chosen. transcriptional activity was observed only upon addition of rabbit reticulocyte lysate, indicating a dependence on translation. under these conditions rna molecules of subgenomic length were synthesized that hybridized to strand-specific probes for the n and nss genes. cloning of these transcripts demonstrated the presence of nonviral leader ... | 2002 | 12490389 |
| expression of the movement protein of tomato spotted wilt virus in its insect vector frankliniella occidentalis. | tomato spotted wilt virus (tswv) is able to infect both its botanical hosts and its insect vector (thrips). in plant tissue the ns(m) protein of tswv functions as viral movement protein (mp), aggregating into plasmodesma-penetrating tubules to establish cell-to-cell movement. as upon heterologous expression ns(m) was able to form similar tubules on the surface of insect (spodoptera frugiperda) cells, we have now investigated the expression and cellular manifestation of this protein in infected t ... | 2002 | 12038691 |
| induction of phenylpropanoid metabolism by pseudomonas fluorescens against tomato spotted wilt virus in tomato. | pseudomonas fluorescens (two native strains, one collection strain and their strain mixtures in all possible combinations) when applied through seed, seedling dip, soil and on leaf significantly reduced the tomato spotted wilt virus (tswv) disease. in p. fluorescens-treated plants, the peroxidase and phenylalanine ammonia-lyase activity increased. accumulation of phenolic compounds and lignin were shown to be increased in the p. fluorescens-treated plants. isoperoxidase native page indicated tha ... | 2002 | 12058389 |
| evaluation of metabolic detoxifying enzyme activities and insecticide resistance in frankliniella occidentalis. | the western flower thrips frankliniella occidentalis (pergande) is a very significant pest of a number of different agricultural crops in the south-east of spain. the importance of thrips as a pest is not due mainly to the direct damage inflicted on the plant, but to the loss in commercial value which occurs as a consequence of the development of dark spots caused by the tomato spotted wilt virus (tswv) which they transmit. the economic threshold is therefore almost zero, which enhances the prob ... | 2002 | 12233183 |
| the route of tomato spotted wilt virus inside the thrips body in relation to transmission efficiency. | the route of tomato spotted wilt virus (tswv) in the body of its vectors, frankliniella occidentalis and thrips tabaci (thysanoptera: thripidae) was studied during their development. first instar larvae were allowed, immediately upon hatching, to acquire virus from mechanically infected datura stramonium plants for 24 h. the rate of transmission by adults was determined in inoculation access feeding test on emilia sonchifolia leaf disks. thrips tissues were analysed for infection at 24 h interva ... | 2002 | 12417949 |
| factors determining vector competence and specificity for transmission of tomato spotted wilt virus. | the competence of a frankliniella occidentalis and a thrips tabaci population to transmit tomato spotted wilt virus (tswv) was analysed. adults of the f. occidentalis population transmitted this virus efficiently, whereas those of the thelytokous t. tabaci population failed to transmit. tswv replicated in the midgut of the larvae of both populations after ingestion of virus; however, lower amounts accumulated in t. tabaci larvae than in f. occidentalis larvae. the virus was almost undetectable i ... | 2002 | 11842261 |
| the detection of tomato spotted wilt virus (tswv) in individual thrips using real time fluorescent rt-pcr (taqman). | tomato spotted wilt virus (tswv) is an important virus, economically in the uk, causing damaging disease in ornamental and vegetable crops. the virus is vectored by several species of thrips, most importantly the western flower thrips (frankliniella occidentalis pergande [thysanoptera: thripidae]). the vector thrips themselves constitute a damaging pest and are difficult to control completely. monitoring thrips numbers is an important part of the control of virus, but does not give information o ... | 2002 | 11849682 |
| cross-talk between jasmonate and salicylate plant defense pathways: effects on several plant parasites. | plants are often attacked by many herbivorous insects and pathogens at the same time. two important suites of responses to attack are mediated by plant hormones, jasmonate and salicylate, which independently provide resistance to herbivorous insects and pathogens, respectively. several lines of evidence suggest that there is negative cross-talk between the jasmonate and salicylate response pathways. this biochemical link between general plant defense strategies means that deploying defenses agai ... | 2002 | 28547690 |
| [changes in ultrastructure and protein metabolism of tobacco plants infected by tomato spotted wilt virus]. | as the result of electron microscope investigation of ultra-thin sections of the tissues infected by tomato spotted wilt virus it was shown that ultrastructural changes in the cells depend on the virus virulence. the isolate with low virulence induces mostly virus-specific changes (virus particles and virus inclusion bodies); the isolate with high virulence besides the virus-specific changes causes essential non-specific violation of cell organelle structure that could be the consequence of path ... | 2003 | 12945179 |
| association of l protein and in vitro tomato spotted wilt virus rna-dependent rna polymerase activity. | we have previously described an in vitro assay for rna-dependent rna polymerase activity in virions of tomato spotted wilt virus (tswv). here we report antibody inhibition of virion-associated rna synthesis in vitro with an l-protein-specific polyclonal antibody raised against the carboxy-terminus of the l protein. in contrast, rna synthesis was not inhibited by a heterologous antiserum and was unaffected by antiserum raised against an internal portion of the l protein. our results directly asso ... | 2003 | 12867756 |
| negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. | posttranscriptional silencing of a green fluorescent protein (gfp) transgene in nicotiana benthamiana plants was suppressed when these plants were infected with tomato spotted wilt virus (tswv), a plant-infecting member of the bunyaviridae: infection with tswv resulted in complete reactivation of gfp expression, similar to the case for potato virus y, but distinct from that for cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. agrobacterium-based lea ... | 2003 | 12502849 |
| an unusual large intergenic region in the s-rna of a bulgarian tomato spotted wilt virus isolate. | the complete s-rna sequences of four bulgarian and one german isolate of tomato spotted wilt tospovirus were determined. all isolates show a high conservation in their n proteins, while the nss proteins and the intergenic regions (igr) are more variable. the bulgarian isolate 10hk96 has the largest s-rna (3364 nucleotides) among tomato spotted wilt viruses reported so far. the enlargement is based on an insertion of 365 nts in the igr that may have resulted from stuttering of the viral polymeras ... | 2003 | 12536305 |
| seasonal dispersal patterns of frankliniella fusca (thysanoptera: thripidae) and tomato spotted wilt virus occurrence in central and eastern north carolina. | the seasonal abundance and temporal pattern of frankliniella fusca hinds dispersal were monitored from 1996 to 2000 at 12 locations in central and eastern north carolina. the predominant vector species of tomato spotted wilt virus (tswv) captured across all locations was f. fusca (98%). the temporal patterns of f. fusca dispersal observed during spring seasons varied among locations in all years except 2000. regression analysis estimated that times of first flight in the spring seasons varied am ... | 2003 | 12650337 |
| detection of tomato spotted wilt virus in its vector frankliniella occidentalis by reverse transcription-polymerase chain reaction. | a method for rapid and reliable detection of tomato spotted wilt virus (tswv) (tospovirus, bunyaviridae) in its vector frankliniella occidentalis (thysanoptera thripidae) would be a useful tool for studying the epidemiology of this virus. a rt-pcr method developed for this purpose is reported. the method was tested on thrips involved in laboratory transmission trials and on thrips collected in the field, whose capability to transmit tswv was checked previously by leaf disk assays. the rt-pcr res ... | 2003 | 12668270 |
| peptide-mediated broad-spectrum plant resistance to tospoviruses. | plant viruses have a significant impact on agronomic losses worldwide. a new strategy for engineering virus-resistant plants by transgenic expression of a dominant interfering peptide is presented here. this peptide of 29 aa strongly interacts with the nucleocapsid proteins (n) of different tospoviruses. transgenic nicotiana benthamiana lines expressing the peptide fused to a carrier protein were challenged with five different tospoviruses that have a nucleocapsid protein interacting with the pe ... | 2003 | 12682295 |
| epidemiology and management of tomato spotted wilt in peanut. | tomato spotted wilt caused by thrips-vectored tomato spotted wilt virus (tswv) is a very serious problem in peanut (arachis hypogaea l.) production. tswv and the thrips frankliniella fusca and frankliniella occidentalis, which vector the virus, present a difficult and complicated challenge from the perspectives of both epidemiology and disease management. simply controlling the vector typically has not resulted in control of spotted wilt. no single measure can currently provide adequate control ... | 2003 | 12704217 |
| characterisation of the feeding behaviour of western flower thrips in terms of electrical penetration graph (epg) waveforms. | western flower thrips, frankliniella occidentalis (pergande) causes damage to plants when they are feeding. also, this thrips species transmits tomato spotted wilt virus (tswv) during stylet penetration. we investigated the penetration behaviour (probing) of thrips on pepper leaves and on liquid diet by electrical penetration graph (epg, dc-system) recording. in addition, we used high-magnification video observations to correlate epg waveforms with the insect's posture, head movements, and muscl ... | 2003 | 12769993 |
| integrated management tactics for frankliniella thrips (thysanoptera: thripidae) in field-grown pepper. | in a 2-yr study, the impacts of different plastic soil mulches, insecticides, and predator releases on frankliniella thrips and their natural enemies were investigated in field-grown peppers. ultraviolet light (uv)-reflective mulch significantly reduced early season abundance of adult thrips compared with standard black plastic mulch. this difference diminished as the growing seasons progressed. late season abundance of thrips larvae was higher in uv reflective mulch compared with black mulch pl ... | 2003 | 14503592 |
| selection of tomato plants resistant to a local polish isolate of tomato spotted wilt virus (tswv). | we found that the sw-5 gene confers resistance to one of the polish isolates of tomato spotted wilt virus (tswv). a series of tomato breeding accessions was analysed along with standards of resistance and susceptibility to tswv. the presence of the sw-5 gene was determined using the available pcr marker. subsequently plants from these accessions were grown in the presence of the tswv isolate from poland. some of them developed severe symptoms of the tswv disease. expression of the virus proteins ... | 2003 | 14617826 |
| thrips resistance in pepper and its consequences for the acquisition and inoculation of tomato spotted wilt virus by the western flower thrips. | abstract different levels of thrips resistance were found in seven capsicum accessions. based on the level of feeding damage, host preference, and host suitability for reproduction, a thrips susceptible and a resistant accession were selected to study their performance as tomato spotted wilt virus (tswv) sources and targets during thrips-mediated virus transmission. vector resistance did not affect the virus acquisition efficiency in a broad range of acquisition access periods. inoculation effic ... | 2003 | 18944162 |
| restricted spread of tomato spotted wilt virus in thrips-resistant pepper. | abstract spread of tomato spotted wilt virus (tswv) and population development of its vector frankliniella occidentalis were studied on the pepper accessions cpro-1 and pikante reuzen, which are resistant and susceptible to thrips, respectively. viruliferous thrips were released on plants of each accession (nonchoice tests) or on plants in a 1:1 mixture of both accessions (choice tests) in small cages containing 8 or 16 plants. significantly fewer cpro-1 plants became infected in the primary inf ... | 2003 | 18944320 |
| acquisition of tomato spotted wilt virus by adults of two thrips species. | abstract only larval thrips that acquire tomato spotted wilt virus (tswv), or adults derived from such larvae, transmit the virus. nonviruliferous adults can ingest virus particles while feeding on tswv-infected plants, but such adult thrips have not been shown to transmit tswv. immunofluorescence microscopy was used to show that thrips 1, 5, 10, and 20 days after adult emergence (dae) fed on tswv-infected plants acquired tswv with virus replication and accumulation occurring in both epithelial ... | 2004 | 18944108 |
| interaction between tomato spotted wilt virus n protein monomers involves nonelectrostatic forces governed by multiple distinct regions in the primary structure. | abstract the ambisense rna genome of tomato spotted wilt virus (tswv) isby interaction with numerous copies of the virus encoded nucleocapsid (n) protein to form a subvirion structure called a ribonucleo-protein (rnp). rnps are central to the viral replication cycle because they, and not free viral rna, serve as templates for viral gene expression and genome replication. n protein monomers bind to viral rna molecules in a cooperative manner. we have examined regions of the n protein that are inv ... | 2004 | 18943909 |
| occurrence and distribution of lettuce mosaic disease in tehran province from iran. | lettuce mosaic virus (lmv) cucumber mosaic virus (cmv) and tomato spotted wilt virus (tswv) were identified in fields of tehran province. in this study 452 leaf samples were collected from the fields throughout the tehran province during 2002 and 2003. distribution of lettuce mosaic virus (lmv), cucumber mosaic virus (cmv), tomato spotted wilt virus (tswv)and arabis mosaic virus (armv) was determined with das-elisa. percentage of single infection to lmv. cmv or tswv was 20.58, 15.93 and 9.96% re ... | 2004 | 15756832 |
| experimental and natural weed host-virus relations. | weeds, as alternative hosts of plant viruses and nutrient plants of virus vectors play important role in virus ecology and epidemiology. the aim of our study was to discover new weed-virus relations. therefore some weed species were mechanically inoculated with 28 viruses (strains or isolates) maintained in our glasshouse. different weed species with and without visible symptoms were collected from agro-, water ecosystems and wastelands of hungary between 1997 and 2003. virus infections were eva ... | 2004 | 15759395 |
| expression of a modified nucleocapsid-protein of tomato spotted wilt virus (tswv) confers resistance against tswv and groundnut ringspot virus (grsv) by blocking systemic spread. | summary tobacco transformed with a construct comprising the nucleocapsid-protein (n) gene of tomato spotted wilt virus (tswv) and the 5' non-translated leader sequence of plum pox virus (ppv) as a translation enhancer displays unusually broad resistance against tospoviruses. contrary to expectations, the transgenic plants did not express a higher level of tswv n protein than control plants harbouring the n gene without the ppv enhancer. instead, expression of an n-terminally elongated n protein, ... | 2004 | 20565598 |
| tomato spotted wilt virus infection improves host suitability for its vector frankliniella occidentalis. | abstract the effect of tomato spotted wilt virus (tswv) infection on plant attractiveness for the western flower thrips (frankliniella occidentalis) was studied. significantly more thrips were recovered on infected than were recovered on noninfected pepper (capsicum annuum) plants in different preference tests. in addition, more offspring were produced on the virus-infected pepper plants, and this effect also was found for tswv-infected datura stramonium. thrips behavior was minimally influenced ... | 2004 | 18943902 |
| tactics for management of thrips (thysanoptera: thripidae) and tomato spotted wilt virus in tomato. | four studies were conducted in georgia during spring 1999, 2000, 2001, and 2002 to evaluate various management tactics for reducing thrips and thrips-vectored tomato spotted wilt virus (tswv) in tomato and their interactions relative to fruit yield. populations of thrips vectors of tswv, frankliniella occidentalis (pergande) and frankliniella fusca (hinds), were determined using flower and sticky trap samples. the management practices evaluated were host plant resistance, insecticide treatments, ... | 2004 | 15568355 |
| using epidemiological information to develop effective integrated virus disease management strategies. | virus diseases cause serious losses in yield and quality of cultivated plants worldwide. these losses and the resulting financial damage can be limited by controlling epidemics using measures that minimise virus infection sources or suppress virus spread. for each combination of virus, cultivated plant and production system, there is an 'economic threshold' above which the financial damage is sufficient to justify using such measures. however, individual measures used alone may bring only small ... | 2004 | 15036832 |
| [pathogenesis and induced virus resistance in tobacco plants affected by tomato spotted wilt virus]. | pathogenecity and virulence of tomato spotted wilt virus isolated from tobacco were studied under the field conditions of the crimea in the tobacco plants of immunny 580 variety and datura stramonium. as to their virulence the obtained 26 isolates were divided into three groups: strongly virulent (severe), middle-virulent and weakly virulent ("mosaic"). it has been shown that strongly virulent isolates interfere with weakly virulent ones and induce the development of nonspecific resistance in to ... | 2004 | 15208858 |
| the two envelope membrane glycoproteins of tomato spotted wilt virus show differences in lectin-binding properties and sensitivities to glycosidases. | tomato spotted wilt virus (tswv, genus: tospovirus, family: bunyaviridae) is a major constraint to the production of several different crops of agronomic and horticultural importance worldwide. the amino acid sequence of the two envelope membrane glycoproteins, designated as g(n) (n-terminal) and g(c) (c-terminal), of tswv contain several tripeptide sequences, asn-xaa-ser/thr, suggesting that the proteins are n-glycosylated. in this study, the lectin-binding properties of the viral glycoproteins ... | 2004 | 14967492 |
| in vitro transcription of tomato spotted wilt virus is independent of translation. | ongoing transcription in vitro of tomato spotted wilt virus (tswv) has previously been demonstrated to require the presence of reticulocyte lysate. this dependence was further investigated by testing the occurrence of transcription in the presence of two translation inhibitors: edeine, an inhibitor that still allows scanning of nascent mrnas by the 40s ribosomal subunit, and cycloheximide, an inhibitor that completely blocks translation including ribosome scanning. neither of these inhibitors bl ... | 2004 | 15105551 |
| host-associated genetic differentiation in thrips tabaci (insecta; thysanoptera), as determined from mtdna sequence data. | we tested for host-associated genetic differentiation in 22 populations of thrips tabaci collected from tobacco and leek, respectively. clustering analyses and haplotype networks based on sequence variation at a fragment of the mitochondrial cytochrome oxidase i gene yielded three major evolutionary lineages; two were clearly associated with leek and the third with tobacco. these genetic findings corroborated recent experimental observations on the heterogeneity of t. tabaci populations with reg ... | 2004 | 15241445 |
| trapping of tomato yellow leaf curl virus (tylcv) and other plant viruses with a groel homologue from the whitefly bemisia tabaci. | to avoid destruction in the haemolymph of their vector, many plant circulative viruses interact with groel homologues produced by insect endosymbiotic bacteria. we have exploited this phenomenon to devise tools allowing trapping of plant viruses by either groel purified from the whitefly bemisia tabaci or by whitefly groel over-expressed in e. coli. pcr tubes or 96-well plates coated with a groel preparation were incubated with cleared sap of virus infected plant leaves or insect vectors. groel- ... | 2004 | 15290375 |
| development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum. | an immunocapture reverse transcription loop-mediated isothermal amplification (ic/rt-lamp) was developed for the detection of tomato spotted wilt virus (tswv) from chrysanthemum. this method enabled sensitive, reproducible and specific detection of tswv from chrysanthemum plants. in the rt-lamp method, tswv genomic rna could be amplified under isothermal (65 degrees c) conditions within 1 h. the resulting amplicons were detected by the measurement or observation of the turbidity of the reaction ... | 2004 | 15350732 |
| specificity of accumulation and transmission of tomato spotted wilt virus (tswv) in two genera, frankliniella and thrips (thysanoptera: thripidae). | the accumulation and transmission of tomato spotted wilt virus (tswv) was examined in second instar larvae and adults of two thrips genera, frankliniella and thrips. the species tested were f. occidentalis (pergande), f. intonsa (trybom), t. tabacilindeman, t. setosus moulton, t. palmi karny and t. hawaiiensis (morgan). in a standard petunia leaf disc assay, the efficiencies of tswv transmission by two species of frankliniella were higher than those of any thrips species in the adult stage. a tr ... | 2004 | 15541189 |
| expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein gn. | tomato spotted wilt virus (tswv), a member of the tospovirus genus within the bunyaviridae, is an economically important plant pathogen with a worldwide distribution. tswv is transmitted to plants via thrips (thysanoptera: thripidae), which transmit the virus in a persistent propagative manner. the envelope glycoproteins, g(n) and g(c), are critical for the infection of thrips, but they are not required for the initial infection of plants. thus, it is assumed that the envelope glycoproteins play ... | 2004 | 15542672 |
| sequence analysis of the glycoproteins of tomato chlorotic spot virus and groundnut ringspot virus and comparison with other tospoviruses. | the tospoviruses tomato chlorotic spot virus (tcsv) and groundnut ringspot virus (grsv) cause high economic losses in several vegetable crops in brazil. the glycoprotein precursor coding sequence was still not available for these two viruses. in this study, the 3' 4 kb m rna of tcsv and grsv genome was cloned and sequenced. the sequences were compiled with the available 5' region sequence (nsm gene and 5' utr) of the same isolates. the m rna of tcsv was deduced as formed by 4,882 nucleotides, wh ... | 2004 | 15550772 |
| the plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, frankliniella occidentalis. | tospoviruses have the ability to infect plants and their insect vectors. tomato spotted wilt virus (tswv), the type species in the tospovirus genus, infects its most important insect vector, frankliniella occidentalis, the western flower thrips (wft). however, no detrimental effects on the life cycle or cytopathological changes have been reported in the wft after tswv infection, and relatively few viral particles can be observed even several days after infection. we hypothesized that tswv infect ... | 2004 | 15113877 |
| la crosse virus nonstructural protein nss counteracts the effects of short interfering rna. | through a process known as rna interference (rnai), double-stranded short interfering rnas (sirnas) silence gene expression in a sequence-specific manner. recently, several viral proteins, including the nonstructural protein nss of tomato spotted wilt virus (a plant-infecting bunyavirus), the interferon antagonist protein ns1 of influenza virus, and the e3l protein of vaccinia virus, have been shown to function as suppressors of rnai, presumably as a counterdefense against cellular mechanisms th ... | 2005 | 15596819 |
| tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementarity to the viral template. | transcription of segmented negative-strand rna viruses is initiated by cap snatching: a host mrna is cleaved generally at 10-20 nt from its 5' capped end and the resulting capped leader used to prime viral transcription. for tomato spotted wilt virus (tswv), type species of the plant-infecting tospovirus genus within the bunyaviridae, cap donors were previously shown to require a single base complementarity to the ultimate or penultimate viral template sequence. more recently, the occurrence in ... | 2005 | 15823611 |
| the use of fluorescence microscopy to visualise homotypic interactions of tomato spotted wilt virus nucleocapsid protein in living cells. | fluorescence resonance energy transfer (fret) and fluorescence lifetime imaging microscopy (flim) were employed to study homotypic protein-protein interactions in living cells. to this end, the nucleocapsid (n) protein of tomato spotted wilt virus (tswv) was expressed as a fusion protein with either cyan fluorescent protein (cfp) or yellow fluorescent protein (yfp). co-expression experiments of the two fusion proteins were carried out in baby hamster kidney (bhk21) cells. both the wild type and ... | 2005 | 15737412 |
| viral genetic determinants for thrips transmission of tomato spotted wilt virus. | tomato spotted wilt virus (tswv) is transmitted exclusively by thrips in nature. a reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (m) rna of tswv. to locate determinants of thrips transmission in the m rna, 30 single-lesion isolates (slis) were generated from a single tswv isolate that was inefficiently transmitted by thrips. three of the 30 slis were transmitted by thrips, and 27 were not. sequence analysis of the m rna, thrips transmissibility ... | 2005 | 15753307 |
| the molecular population genetics of the tomato spotted wilt virus (tswv) genome. | rna viruses are characterized by high genetic variability resulting in rapid adaptation to new or resistant hosts. research for plant rna virus genetic structure and its variability has been relatively scarce compared to abundant research done for human and animal rna viruses. here, we utilized a molecular population genetic framework to characterize the evolution of a highly pathogenic plant rna virus [tomato spotted wilt virus (tswv), tospovirus, bunyaviridae]. data from genes encoding five vi ... | 2005 | 15643950 |
| tomato spotted wilt virus s-segment mrnas have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif. | the tomato spotted wilt virus ambisense m- and s-rna segments contain an a/u-rich intergenic region predicted to form a stable hairpin structure. the site of transcription termination of s-segment encoded n and nss mrnas synthesised in an in vitro transcription system was roughly mapped to the 3'-end of the intergenic hairpin, i.e. position 1568-1574 for n and position 1852-1839 for nss, as determined by rt-pcr cloning and size estimation on northern blots. this suggests that these viral transcr ... | 2005 | 15845263 |
| tomato spotted wilt virus glycoprotein g(c) is cleaved at acidic ph. | tomato spotted wilt virus (tswv) is a plant-infecting member of the family bunyaviridae. tswv encodes two envelope glycoproteins, g(n) and g(c), which are required for virus infection of the arthropod vector. other members of the bunyaviridae enter host cells by ph-dependent endocytosis. during this process, the glycoproteins are exposed to conditions of acidic ph within endocytic vesicles causing the g(c) protein to change conformation. this conformational change renders g(c) more sensitive to ... | 2005 | 15845270 |
| expression of a viral polymerase-bound host factor turns human cell lines permissive to a plant- and insect-infecting virus. | tospoviruses are the only plant-infecting members of the bunyaviridae family of ambisense ssrna viruses. tomato spotted wilt tospovirus (tswv), the type-member, also causes mild infection on its main insect vector, frankliniella occidentalis. herein, we identified an f. occidentalis putative transcription factor (fotf) that binds to the tswv rna-dependent rna polymerase and to viral rna. using in vitro rna synthesis assays, we show that addition of purified fotf improves viral replication, but n ... | 2005 | 15657123 |
| the tubule-forming nsm protein from tomato spotted wilt virus complements cell-to-cell and long-distance movement of tobacco mosaic virus hybrids. | a florida isolate of tomato spotted wilt virus (tswv) was able to complement cell-to-cell movement of a movement-defective tobacco mosaic virus (tmv) vector expressing the jellyfish green fluorescent protein (gfp). to test for complementation of movement in the absence of other tswv proteins, the open reading frame for the nsm protein was expressed from tmv constructs encoding only the tmv replicase proteins. nsm was expressed from either the coat protein or movement protein subgenomic promoter, ... | 2005 | 16112159 |
| phage display-selected single-chain antibodies confer high levels of resistance against tomato spotted wilt virus. | rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. high cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were e ... | 2005 | 15958689 |
| matrix attachment regions increase the efficiency and stability of rna-mediated resistance to tomato spotted wilt virus in transgenic tobacco. | matrix attachment regions (mars) are dna elements that can increase and stabilize transgene expression. we investigated the effect of the rb7 mar on transgenic virus resistance. constructs for resistance to tomato spotted wilt virus (tswv) with and without flanking rb7 mars were used to transform tobacco and produce homozygous lines. the population with the mar construct had a significantly higher percentage of tswv resistant plants in the r1 generation than the nonmar population. each resistant ... | 2005 | 16022390 |
| management of spotted wilt vectored by frankliniella fusca (thysanoptera: thripidae) in virginia market-type peanut. | field tests were conducted during 2001 and 2002 in northeastern north carolina to evaluate the impact of cultural practices and in-furrow insecticides on the incidence of tomato spotted wilt virus (genus tospovirus, family bunyaviridae, tswv), which is transmitted to peanut, arachis hypogaea l., primarily by tobacco thrips, frankliniella fusca hinds (thysanoptera: thripidae). treatments included in row plant populations of 7, 13, and 17 plants per meter; the virginia market-type 'nc v-11' and 'p ... | 2005 | 16334308 |
| impact of early-season thrips management on reducing the risks of spotted wilt virus and suppressing aphid populations in flue-cured tobacco. | the influence of tray drench (td) treatments, with and without foliar applications of the plant activator acibenzolar-s-methyl (actigard), was examined in replicated field plots in 2000--2002. td treatments of actigard, imidacloprid (admire), and these two products combined had little effect on seasonal mean thrips populations; however, thrips densities were lower in the admire-treated plots at 4 and 5 wk after transplanting. actigard and admire td treatments significantly reduced the seasonal i ... | 2005 | 15765674 |
| tobacco plants respond to the constitutive expression of the tospovirus movement protein ns(m) with a heat-reversible sealing of plasmodesmata that impairs development. | viral infection often results in typical symptoms, the biological background of which has remained elusive. we show that constitutive expression of the nsm viral movement protein (mp) of tomato spotted wilt virus in nicotiana tabacum is sufficient to induce severe, infection-like symptoms, including pronounced deficiencies in root and shoot development. leaves failed to expand and were arranged in a rosette due to the absence of internode elongation. following the sink-source transition they acc ... | 2005 | 16115066 |
| purification and serological analyses of tospoviral nucleocapsid proteins expressed by zucchini yellow mosaic virus vector in squash. | a plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus (zymv) was used to express the nucleocapsid proteins (nps) of tospoviruses in planta. the open reading frames (orfs) of nps of different serogroups of tospoviruses, including tomato spotted wilt virus, impatiens necrotic spot virus, watermelon silver mottle virus, peanut bud necrosis virus, and watermelon bud necrosis virus (wbnv), were in frame inserted in between the p1 and hc-pro genes of the zymv ... | 2005 | 15992936 |
| field evaluation of tomato hybrids engineered with tomato spotted wilt virus sequences for virus resistance, agronomic performance, and pollen-mediated transgene flow. | abstract tomato hybrids obtained from homozygous progeny of line 30-4, engineered for tomato spotted wilt virus (tswv) resistance, were tested under field conditions in two locations with their corresponding nontransgenic hybrids. no transgenic hybrid became infected, but 33 to 50% of plants of each nontransgenic hybrid became infected with a severe reduction of marketable fruit production. the transgenic hybrids conformed to the standard agronomic characteristics of the corresponding nontransge ... | 2005 | 18943013 |
| tomato spotted wilt virus particle assembly and the prospects of fluorescence microscopy to study protein-protein interactions involved. | 2005 | 16387194 | |
| [some biologic characteristics of two tomato spotted wilt virus isolates]. | biological properties of two tomato spotted wilt virus (tswv) isolates from the tomatoes, grown on plantations of the crimean tobacco-plant experimental stations have been investigated. these isolates were slightly differentiated by thermal inactivation point, by terms of virus storage in juice and by dilution end point. as a result the growth of the induced resistance effective with respect to tmv was observed in tobacco-plants of immunny-580 and n. sanderae varieties affected by tswv. | 2005 | 16396117 |
| host plant, temperature, and photoperiod effects on ovipositional preference of frankliniella occidentalis and frankliniella fusca (thysanoptera: thripidae). | host plant effects of tomato, lycopersicon esculentum mill., and chickweed, stellaria media (l.) vill., foliage infected and uninfected with tomato spotted wilt virus (family bunyaviridae, genus tospovirus, tswv) on the ovipositional preferences of western flower thrips, frankliniella occidentalis (pergande), and tobacco thrips, frankliniella fusca (hinds), were investigated for whole plants in the greenhouse. in addition, the preference for leaf disks from the same host plants was investigated ... | 2005 | 16539139 |
| first report of occurrence of two viruses on pea field in iran. | an intensive survey was conducted to identify virus diseases affecting pea crops in tehran province of iran. a total of 270 pea samples were collected randomly from pea fields. samples were tested by double antibody sandwich enzyme linked immunosorbent assay (das-elisa) using polyclonal antisera prepared against psbmv (as-0129, dsmz, braunschweig, germany) and tswv (as-0580, dsmz, braunschweig, germany). virus disease incidence in pea samples was followed by psbmv (33%) tswv (24.4%) and psbmv+ts ... | 2005 | 16637207 |
| a one-step reverse transcription-polymerase chain reaction system for the simultaneous detection and identification of multiple tospovirus infections. | abstract a one-step reverse transcription-polymerase chain reaction (rt-pcr) method has been developed for the simultaneous detection and identification of multiple tospoviruses that infect plants. the rt-pcr system is composed of six primers in a single tube: a universal degenerate primer and five virus species-specific primers. amplifications resulted in an 848-bp pcr product for watermelon silver mottle virus, 709-bp for tomato spotted wilt virus, 589-bp for impatiens necrotic spot virus, 511 ... | 2005 | 18943986 |
| synergistic interaction between tomato chlorosis virus and tomato spotted wilt virus results in breakdown of resistance in tomato. | abstract multiple viral infections frequently are found in single plants of cultivated and wild hosts in nature, with unpredictable pathological consequences. synergistic reactions were observed in mixed infections in tomato plants doubly infected with the positive-sense and phloem-limited single-stranded rna (ssrna) crinivirus tomato chlorosis virus (tocv) and the negative-sense ssrna tospovirus tomato spotted wilt virus (tswv). synergism in a tomato cultivar susceptible to both viruses resulte ... | 2006 | 18943964 |
| viral suppressors of rna interference impair rna silencing induced by a semliki forest virus replicon in tick cells. | it was recently shown that infection of ise6 tick cells by a recombinant semliki forest virus (sfv) expressing a heterologous gene induced small interfering rnas (sirnas) and silencing of the gene. to gain information on rna interference (rnai) in ticks, three known viral inhibitors that act in different ways, the ns1 protein of influenza virus, nss of tospovirus tomato spotted wilt virus and hc-pro of zucchini yellow mosaic virus were expressed and investigated to determine if they antagonize i ... | 2006 | 16760400 |
| at-4/1, an interactor of the tomato spotted wilt virus movement protein, belongs to a new family of plant proteins capable of directed intra- and intercellular trafficking. | the tomato spotted wilt virus (tswv) encoded nsm movement protein facilitates cell-to-cell spread of the viral genome through structurally modified plasmodesmata. nsm has been utilized as bait in yeast two-hybrid interaction trap screenings. as a result, a protein of unknown function, called at-4/1, was isolated from an arabidopsis thaliana gal4 activation domain-tagged cdna library. using polyclonal antibodies against bacterially expressed at-4/1, western blot analysis of protein extracts isola ... | 2006 | 16903353 |
| relationship between esterase activity and acrinathrin and methiocarb resistance in field populations of western flower thrips, frankliniella occidentalis. | the western flower thrips, frankliniella occidentalis (pergande), is a serious pest in the south-east of spain owing to its direct feeding on crops, transmission of the tomato spotted wilt virus and its very high level of resistance to insecticides. mechanisms of resistance were examined using field populations of f. occidentalis with different susceptibilities to acrinathrin, methiocarb (selective insecticides), endosulfan, metamidophos and deltamethrin (broad-spectrum insecticides). esterase a ... | 2006 | 16929461 |
| high incidence of tobacco streak virus in the tobacco fields of iran. | currently the northern provinces of iran (mazandaran, golestan and gilan) are the main tobacco growing regions in the country and this crop has an special importance in the national economy. three tobacco types including flue-cured (mainly coker 347 and some virginia e1), burley (burley 21) and oriental (basma 178-2) are presently grown in these regions. epidemics of viral diseases have occurred during the recent years in many tobacco fields in these areas. the quality of tobacco products which ... | 2006 | 17390881 |
| occurrence of viruses infecting pea in iran. | a survey was conducted to determine the incidence of alfalfa mosaic virus (amv), bean yellow mosaic virus (bymv), broad bean wilt virus-1 (bbwv), pea leafroll virus (plrv), pea enation mosaic virus (pemv), pea seed borne mosaic virus (psbmv), potato virus x(pvx), tomato mosaic virus (tomv), tomato spotted wilt virus (tswv) on pea (pisum sativum) in iran. a total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in tehran province of iran, whe ... | 2006 | 17390891 |
| role of insecticides in reducing thrips injury to plants and incidence of tomato spotted wilt virus in virginia market-type peanut. | tomato spotted wilt virus (family bunyaviridae, genus tospovirus, tswv), transmitted by many thrips species, is a devastating pathogen of peanut, arachis hypogaea l. tswv has become a serious problem in the virginia/carolina peanut-growing region of the united states. during 2002, tswv was present in 47% of the north carolina hectarage and caused a 5% yield reduction in virginia. factors influencing levels of tswv in runner market-type peanut cultivars, which are primarily grown in alabama, flor ... | 2007 | 17849876 |
| tomato spotted wilt virus gc and n proteins interact in vivo. | tomato spotted wilt virus (tswv) virions consist of a nucleocapsid core surrounded by a membrane containing glycoproteins gn and gc. to unravel the protein interactions involved in the membrane acquisition of rnps, tswv nucleocapsid protein (n), gn and gc were expressed and analyzed in bhk21 cells. upon coexpression of gn, gc and n, a partial colocalization of n with both glycoproteins was observed in the golgi region. in contrast, upon coexpression of gc and n in the absence of gn, both protein ... | 2007 | 16963098 |
| interrelationship of phytoalexin production and disease resistance in selected peanut genotypes. | in peanuts, a mechanism of resistance to fungal infection is reportedly due to the synthesis of stilbene phytoalexins, which are antibiotic, low molecular weight metabolites. the phytoalexin-associated response of different peanut genotypes to exogenous invasion in the field has not been investigated and may be useful for breeding resistant peanut cultivars. five peanut genotypes, georgia green, tifton 8, c-99r, gk-7 high oleic, and marc i, which differ in resistance to major peanut diseases, we ... | 2007 | 17326657 |
| evidence for plant viruses in the region of argentina islands, antarctica. | this work focused on the assessment of plant virus occurrence among primitive and higher plants in the antarctic region. sampling occurred during two seasons (2004/5 and 2005/6) at the ukrainian antarctic station 'academician vernadskiy' positioned on argentina islands. collected plant samples of four moss genera (polytrichum, plagiatecium, sanionia and barbilophozia) and one higher monocot plant species, deschampsia antarctica, were further subjected to enzyme-linked immunosorbent assay to test ... | 2007 | 17328120 |
| the cytoplasmic domain of tomato spotted wilt virus gn glycoprotein is required for golgi localisation and interaction with gc. | envelopment of tomato spotted wilt virus nucleocapsids occurs at the golgi stacks of infected cells. this is also the place where the two membrane glycoproteins gn and gc accumulate upon coexpression. the required golgi retention signal has previously been demonstrated to reside within gn. using a series of truncated gn proteins, the golgi retention signal was mapped to a stretch of 10 amino acids on this protein's cytoplasmic tail, 20 residues downstream the transmembrane domain. studies on the ... | 2007 | 17331557 |
| the glycoprotein gene of chrysanthemum stem necrosis virus and zucchini lethal chlorosis virus and molecular relationship with other tospoviruses. | two tospoviruses, chrysanthemum stem necrosis virus (csnv) and zucchini lethal chlorosis virus (zlcv), cause economical losses in several ornamental and vegetable crops in brazil. the nucleocapsid gene and movement protein sequences had already been reported for both viruses, though the glycoprotein precursor gene sequence was not available. in this study, cdna fragments (ca. 4 kb) of the m rna 3' portion of csnv (isolate chry-1) and zlcv (isolate 1038), including the complete glycoprotein precu ... | 2007 | 17570049 |
| the nucleoprotein of tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plants. | upon infection, tomato spotted wilt virus (tswv) forms ribonucleoprotein particles (rnps) that consist of nucleoprotein (n) and viral rna. these aggregates result from the homopolymerization of the n protein, and are highly stable in plant cells. these properties feature the n protein as a potentially useful protein fusion partner. to evaluate this potential, the n protein was fused to the aequorea victoria green fluorescent protein (gfp), either at the amino or carboxy terminus, and expressed i ... | 2007 | 17532227 |
| evidence that the nonstructural protein of tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the tsw gene. | all known pepper cultivars resistant to tomato spotted wilt virus (tswv) possess a single dominant resistance gene, tsw. recently, naturally occurring resistance-breaking (rb) tswv strains have been identified, causing major concerns. we used a collection of such strains to identify the specific genetic determinant that allows the virus to overcome the tsw gene in capsicum spp. a reverse genetic approach is still not feasible for tswv; therefore, we analyzed reassortants between wild-type (wt) a ... | 2007 | 17506332 |
| a predictive model for spotted wilt epidemics in peanut based on local weather conditions and the tomato spotted wilt virus risk index. | tomato spotted wilt virus (tswv), a member of the genus tospovirus (family bunyaviridae), is an important plant virus that causes severe damage to peanut (arachis hypogaea) in the southeastern united states. disease severity has been extremely variable in individual fields in georgia, due to several factors including variability in weather patterns. a tswv risk index has been developed by the university of georgia to aid peanut growers with the assessment and avoidance of high risk situations. t ... | 2008 | 18943452 |
| herbivore benefits from vectoring plant virus through reduction of period of vulnerability to predation. | herbivores can profit from vectoring plant pathogens because the induced defence of plants against pathogens sometimes interferes with the induced defence of plants against herbivores. plants can also defend themselves indirectly by the action of the natural enemies of the herbivores. it is unknown whether the defence against pathogens induced in the plant also interferes with the indirect defence against herbivores mediated via the third trophic level. we previously showed that infection of pla ... | 2008 | 18392858 |
| a soluble form of the tomato spotted wilt virus (tswv) glycoprotein g(n) (g(n)-s) inhibits transmission of tswv by frankliniella occidentalis. | tomato spotted wilt virus (tswv) is an economically important virus that is transmitted in a persistent propagative manner by its thrips vector, frankliniella occidentalis. previously, we found that a soluble form of the envelope glycoprotein g(n) (g(n)-s) specifically bound thrips midguts and reduced the amount of detectable virus inside midgut tissues. the aim of this research was to (i) determine if g(n)-s alters tswv transmission by thrips and, if so, (ii) determine the duration of this effe ... | 2008 | 18943237 |
| biological and molecular analyses of the acibenzolar-s-methyl-induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus. | tomato spotted wilt virus (tswv) is an economically important virus of flue-cured tobacco. activation of systemic acquired resistance (sar) by acibenzolar-s-methyl (asm) in flue-cured tobacco was studied under greenhouse conditions by challenge inoculation with a severe isolate of tswv. asm restricted virus replication and movement, and as a result reduced systemic infection. activation of resistance was observed within 2 days after treatment with asm and a high level of resistance was observed ... | 2008 | 18943196 |
| investigating recombinant protein exudation from roots of transgenic tobacco. | it is widely acknowledged that plant-made pharmaceuticals (pmps) offer numerous benefits, including inexpensive production, biological safety and the facility for production at agricultural scale. at the same time, it is important to minimize any potential risk associated with this new technology, including the potential release of bioactive proteins into the environment. to address this issue, we studied transgenic nicotiana benthamiana and nicotiana tabacum plants expressing two recombinant si ... | 2008 | 19081009 |