| electron microscopy of vira-cabeca virus (brazilian tomato spotted wilt virus) within the host cell. | | 1965 | 14293317 |
| evaluation of metabolic detoxifying enzyme activities and insecticide resistance in frankliniella occidentalis. | the western flower thrips frankliniella occidentalis (pergande) is a very significant pest of a number of different agricultural crops in the south-east of spain. the importance of thrips as a pest is not due mainly to the direct damage inflicted on the plant, but to the loss in commercial value which occurs as a consequence of the development of dark spots caused by the tomato spotted wilt virus (tswv) which they transmit. the economic threshold is therefore almost zero, which enhances the prob ... | 2002 | 12233183 |
| the route of tomato spotted wilt virus inside the thrips body in relation to transmission efficiency. | the route of tomato spotted wilt virus (tswv) in the body of its vectors, frankliniella occidentalis and thrips tabaci (thysanoptera: thripidae) was studied during their development. first instar larvae were allowed, immediately upon hatching, to acquire virus from mechanically infected datura stramonium plants for 24 h. the rate of transmission by adults was determined in inoculation access feeding test on emilia sonchifolia leaf disks. thrips tissues were analysed for infection at 24 h interva ... | 2002 | 12417949 |
| induction of phenylpropanoid metabolism by pseudomonas fluorescens against tomato spotted wilt virus in tomato. | pseudomonas fluorescens (two native strains, one collection strain and their strain mixtures in all possible combinations) when applied through seed, seedling dip, soil and on leaf significantly reduced the tomato spotted wilt virus (tswv) disease. in p. fluorescens-treated plants, the peroxidase and phenylalanine ammonia-lyase activity increased. accumulation of phenolic compounds and lignin were shown to be increased in the p. fluorescens-treated plants. isoperoxidase native page indicated tha ... | 2002 | 12058389 |
| epidemiology and management of tomato spotted wilt in peanut. | tomato spotted wilt caused by thrips-vectored tomato spotted wilt virus (tswv) is a very serious problem in peanut (arachis hypogaea l.) production. tswv and the thrips frankliniella fusca and frankliniella occidentalis, which vector the virus, present a difficult and complicated challenge from the perspectives of both epidemiology and disease management. simply controlling the vector typically has not resulted in control of spotted wilt. no single measure can currently provide adequate control ... | 2003 | 12704217 |
| characterisation of the feeding behaviour of western flower thrips in terms of electrical penetration graph (epg) waveforms. | western flower thrips, frankliniella occidentalis (pergande) causes damage to plants when they are feeding. also, this thrips species transmits tomato spotted wilt virus (tswv) during stylet penetration. we investigated the penetration behaviour (probing) of thrips on pepper leaves and on liquid diet by electrical penetration graph (epg, dc-system) recording. in addition, we used high-magnification video observations to correlate epg waveforms with the insect's posture, head movements, and muscl ... | 2003 | 12769993 |
| association of l protein and in vitro tomato spotted wilt virus rna-dependent rna polymerase activity. | we have previously described an in vitro assay for rna-dependent rna polymerase activity in virions of tomato spotted wilt virus (tswv). here we report antibody inhibition of virion-associated rna synthesis in vitro with an l-protein-specific polyclonal antibody raised against the carboxy-terminus of the l protein. in contrast, rna synthesis was not inhibited by a heterologous antiserum and was unaffected by antiserum raised against an internal portion of the l protein. our results directly asso ... | 2003 | 12867756 |
| identification of a novel rna silencing suppressor, nss protein of tomato spotted wilt virus. | rna silencing or post-transcriptional gene silencing (ptgs) in plants is known as a defense system against virus infection. several plant viruses have been shown to encode an rna silencing suppressor. using a green fluorescent protein-based transient suppression assay, we show that nss protein of tomato spotted wilt virus (tswv) has rna silencing suppressor activity. tswv nss protein suppressed sense transgene-induced ptgs but did not suppress inverted repeat transgene-induced ptgs. tswv nss pro ... | 2002 | 12459466 |
| physiological studies on thrips in relation to transmission of tomato spotted wilt virus. | | 1954 | 13219035 |
| cross protection by strains of tomato spotted wilt virus and a new theory to explain it. | | 1954 | 13229839 |
| further evidence for the transfer of character-determinants (recombination) between strains of tomato spotted wilt virus. | | 1955 | 13317855 |
| purification and electron microscopy of tomato spotted-wilt virus. | | 1963 | 13971368 |
| development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum. | an immunocapture reverse transcription loop-mediated isothermal amplification (ic/rt-lamp) was developed for the detection of tomato spotted wilt virus (tswv) from chrysanthemum. this method enabled sensitive, reproducible and specific detection of tswv from chrysanthemum plants. in the rt-lamp method, tswv genomic rna could be amplified under isothermal (65 degrees c) conditions within 1 h. the resulting amplicons were detected by the measurement or observation of the turbidity of the reaction ... | 2004 | 15350732 |
| tomato spotted wilt virus glycoprotein g(c) is cleaved at acidic ph. | tomato spotted wilt virus (tswv) is a plant-infecting member of the family bunyaviridae. tswv encodes two envelope glycoproteins, g(n) and g(c), which are required for virus infection of the arthropod vector. other members of the bunyaviridae enter host cells by ph-dependent endocytosis. during this process, the glycoproteins are exposed to conditions of acidic ph within endocytic vesicles causing the g(c) protein to change conformation. this conformational change renders g(c) more sensitive to ... | 2005 | 15845270 |
| specificity of accumulation and transmission of tomato spotted wilt virus (tswv) in two genera, frankliniella and thrips (thysanoptera: thripidae). | the accumulation and transmission of tomato spotted wilt virus (tswv) was examined in second instar larvae and adults of two thrips genera, frankliniella and thrips. the species tested were f. occidentalis (pergande), f. intonsa (trybom), t. tabacilindeman, t. setosus moulton, t. palmi karny and t. hawaiiensis (morgan). in a standard petunia leaf disc assay, the efficiencies of tswv transmission by two species of frankliniella were higher than those of any thrips species in the adult stage. a tr ... | 2004 | 15541189 |
| expression and characterization of a soluble form of tomato spotted wilt virus glycoprotein gn. | tomato spotted wilt virus (tswv), a member of the tospovirus genus within the bunyaviridae, is an economically important plant pathogen with a worldwide distribution. tswv is transmitted to plants via thrips (thysanoptera: thripidae), which transmit the virus in a persistent propagative manner. the envelope glycoproteins, g(n) and g(c), are critical for the infection of thrips, but they are not required for the initial infection of plants. thus, it is assumed that the envelope glycoproteins play ... | 2004 | 15542672 |
| sequence analysis of the glycoproteins of tomato chlorotic spot virus and groundnut ringspot virus and comparison with other tospoviruses. | the tospoviruses tomato chlorotic spot virus (tcsv) and groundnut ringspot virus (grsv) cause high economic losses in several vegetable crops in brazil. the glycoprotein precursor coding sequence was still not available for these two viruses. in this study, the 3' 4 kb m rna of tcsv and grsv genome was cloned and sequenced. the sequences were compiled with the available 5' region sequence (nsm gene and 5' utr) of the same isolates. the m rna of tcsv was deduced as formed by 4,882 nucleotides, wh ... | 2004 | 15550772 |
| peptide-mediated broad-spectrum plant resistance to tospoviruses. | plant viruses have a significant impact on agronomic losses worldwide. a new strategy for engineering virus-resistant plants by transgenic expression of a dominant interfering peptide is presented here. this peptide of 29 aa strongly interacts with the nucleocapsid proteins (n) of different tospoviruses. transgenic nicotiana benthamiana lines expressing the peptide fused to a carrier protein were challenged with five different tospoviruses that have a nucleocapsid protein interacting with the pe ... | 2003 | 12682295 |
| detection of tomato spotted wilt virus in its vector frankliniella occidentalis by reverse transcription-polymerase chain reaction. | a method for rapid and reliable detection of tomato spotted wilt virus (tswv) (tospovirus, bunyaviridae) in its vector frankliniella occidentalis (thysanoptera thripidae) would be a useful tool for studying the epidemiology of this virus. a rt-pcr method developed for this purpose is reported. the method was tested on thrips involved in laboratory transmission trials and on thrips collected in the field, whose capability to transmit tswv was checked previously by leaf disk assays. the rt-pcr res ... | 2003 | 12668270 |
| [changes in ultrastructure and protein metabolism of tobacco plants infected by tomato spotted wilt virus]. | as the result of electron microscope investigation of ultra-thin sections of the tissues infected by tomato spotted wilt virus it was shown that ultrastructural changes in the cells depend on the virus virulence. the isolate with low virulence induces mostly virus-specific changes (virus particles and virus inclusion bodies); the isolate with high virulence besides the virus-specific changes causes essential non-specific violation of cell organelle structure that could be the consequence of path ... | 2003 | 12945179 |
| tomato spotted wilt virus particle assembly and the prospects of fluorescence microscopy to study protein-protein interactions involved. | | 2005 | 16387194 |
| [some biologic characteristics of two tomato spotted wilt virus isolates]. | biological properties of two tomato spotted wilt virus (tswv) isolates from the tomatoes, grown on plantations of the crimean tobacco-plant experimental stations have been investigated. these isolates were slightly differentiated by thermal inactivation point, by terms of virus storage in juice and by dilution end point. as a result the growth of the induced resistance effective with respect to tmv was observed in tobacco-plants of immunny-580 and n. sanderae varieties affected by tswv. | 2005 | 16396117 |
| selection of tomato plants resistant to a local polish isolate of tomato spotted wilt virus (tswv). | we found that the sw-5 gene confers resistance to one of the polish isolates of tomato spotted wilt virus (tswv). a series of tomato breeding accessions was analysed along with standards of resistance and susceptibility to tswv. the presence of the sw-5 gene was determined using the available pcr marker. subsequently plants from these accessions were grown in the presence of the tswv isolate from poland. some of them developed severe symptoms of the tswv disease. expression of the virus proteins ... | 2003 | 14617826 |
| using epidemiological information to develop effective integrated virus disease management strategies. | virus diseases cause serious losses in yield and quality of cultivated plants worldwide. these losses and the resulting financial damage can be limited by controlling epidemics using measures that minimise virus infection sources or suppress virus spread. for each combination of virus, cultivated plant and production system, there is an 'economic threshold' above which the financial damage is sufficient to justify using such measures. however, individual measures used alone may bring only small ... | 2004 | 15036832 |
| tactics for management of thrips (thysanoptera: thripidae) and tomato spotted wilt virus in tomato. | four studies were conducted in georgia during spring 1999, 2000, 2001, and 2002 to evaluate various management tactics for reducing thrips and thrips-vectored tomato spotted wilt virus (tswv) in tomato and their interactions relative to fruit yield. populations of thrips vectors of tswv, frankliniella occidentalis (pergande) and frankliniella fusca (hinds), were determined using flower and sticky trap samples. the management practices evaluated were host plant resistance, insecticide treatments, ... | 2004 | 15568355 |
| integrated management tactics for frankliniella thrips (thysanoptera: thripidae) in field-grown pepper. | in a 2-yr study, the impacts of different plastic soil mulches, insecticides, and predator releases on frankliniella thrips and their natural enemies were investigated in field-grown peppers. ultraviolet light (uv)-reflective mulch significantly reduced early season abundance of adult thrips compared with standard black plastic mulch. this difference diminished as the growing seasons progressed. late season abundance of thrips larvae was higher in uv reflective mulch compared with black mulch pl ... | 2003 | 14503592 |
| in vitro transcription of tomato spotted wilt virus is independent of translation. | ongoing transcription in vitro of tomato spotted wilt virus (tswv) has previously been demonstrated to require the presence of reticulocyte lysate. this dependence was further investigated by testing the occurrence of transcription in the presence of two translation inhibitors: edeine, an inhibitor that still allows scanning of nascent mrnas by the 40s ribosomal subunit, and cycloheximide, an inhibitor that completely blocks translation including ribosome scanning. neither of these inhibitors bl ... | 2004 | 15105551 |
| the two envelope membrane glycoproteins of tomato spotted wilt virus show differences in lectin-binding properties and sensitivities to glycosidases. | tomato spotted wilt virus (tswv, genus: tospovirus, family: bunyaviridae) is a major constraint to the production of several different crops of agronomic and horticultural importance worldwide. the amino acid sequence of the two envelope membrane glycoproteins, designated as g(n) (n-terminal) and g(c) (c-terminal), of tswv contain several tripeptide sequences, asn-xaa-ser/thr, suggesting that the proteins are n-glycosylated. in this study, the lectin-binding properties of the viral glycoproteins ... | 2004 | 14967492 |
| the molecular population genetics of the tomato spotted wilt virus (tswv) genome. | rna viruses are characterized by high genetic variability resulting in rapid adaptation to new or resistant hosts. research for plant rna virus genetic structure and its variability has been relatively scarce compared to abundant research done for human and animal rna viruses. here, we utilized a molecular population genetic framework to characterize the evolution of a highly pathogenic plant rna virus [tomato spotted wilt virus (tswv), tospovirus, bunyaviridae]. data from genes encoding five vi ... | 2005 | 15643950 |
| [pathogenesis and induced virus resistance in tobacco plants affected by tomato spotted wilt virus]. | pathogenecity and virulence of tomato spotted wilt virus isolated from tobacco were studied under the field conditions of the crimea in the tobacco plants of immunny 580 variety and datura stramonium. as to their virulence the obtained 26 isolates were divided into three groups: strongly virulent (severe), middle-virulent and weakly virulent ("mosaic"). it has been shown that strongly virulent isolates interfere with weakly virulent ones and induce the development of nonspecific resistance in to ... | 2004 | 15208858 |
| la crosse virus nonstructural protein nss counteracts the effects of short interfering rna. | through a process known as rna interference (rnai), double-stranded short interfering rnas (sirnas) silence gene expression in a sequence-specific manner. recently, several viral proteins, including the nonstructural protein nss of tomato spotted wilt virus (a plant-infecting bunyavirus), the interferon antagonist protein ns1 of influenza virus, and the e3l protein of vaccinia virus, have been shown to function as suppressors of rnai, presumably as a counterdefense against cellular mechanisms th ... | 2005 | 15596819 |
| viral genetic determinants for thrips transmission of tomato spotted wilt virus. | tomato spotted wilt virus (tswv) is transmitted exclusively by thrips in nature. a reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (m) rna of tswv. to locate determinants of thrips transmission in the m rna, 30 single-lesion isolates (slis) were generated from a single tswv isolate that was inefficiently transmitted by thrips. three of the 30 slis were transmitted by thrips, and 27 were not. sequence analysis of the m rna, thrips transmissibility ... | 2005 | 15753307 |
| occurrence and distribution of lettuce mosaic disease in tehran province from iran. | lettuce mosaic virus (lmv) cucumber mosaic virus (cmv) and tomato spotted wilt virus (tswv) were identified in fields of tehran province. in this study 452 leaf samples were collected from the fields throughout the tehran province during 2002 and 2003. distribution of lettuce mosaic virus (lmv), cucumber mosaic virus (cmv), tomato spotted wilt virus (tswv)and arabis mosaic virus (armv) was determined with das-elisa. percentage of single infection to lmv. cmv or tswv was 20.58, 15.93 and 9.96% re ... | 2004 | 15756832 |
| experimental and natural weed host-virus relations. | weeds, as alternative hosts of plant viruses and nutrient plants of virus vectors play important role in virus ecology and epidemiology. the aim of our study was to discover new weed-virus relations. therefore some weed species were mechanically inoculated with 28 viruses (strains or isolates) maintained in our glasshouse. different weed species with and without visible symptoms were collected from agro-, water ecosystems and wastelands of hungary between 1997 and 2003. virus infections were eva ... | 2004 | 15759395 |
| the plant virus tomato spotted wilt tospovirus activates the immune system of its main insect vector, frankliniella occidentalis. | tospoviruses have the ability to infect plants and their insect vectors. tomato spotted wilt virus (tswv), the type species in the tospovirus genus, infects its most important insect vector, frankliniella occidentalis, the western flower thrips (wft). however, no detrimental effects on the life cycle or cytopathological changes have been reported in the wft after tswv infection, and relatively few viral particles can be observed even several days after infection. we hypothesized that tswv infect ... | 2004 | 15113877 |
| the use of fluorescence microscopy to visualise homotypic interactions of tomato spotted wilt virus nucleocapsid protein in living cells. | fluorescence resonance energy transfer (fret) and fluorescence lifetime imaging microscopy (flim) were employed to study homotypic protein-protein interactions in living cells. to this end, the nucleocapsid (n) protein of tomato spotted wilt virus (tswv) was expressed as a fusion protein with either cyan fluorescent protein (cfp) or yellow fluorescent protein (yfp). co-expression experiments of the two fusion proteins were carried out in baby hamster kidney (bhk21) cells. both the wild type and ... | 2005 | 15737412 |
| expression of a viral polymerase-bound host factor turns human cell lines permissive to a plant- and insect-infecting virus. | tospoviruses are the only plant-infecting members of the bunyaviridae family of ambisense ssrna viruses. tomato spotted wilt tospovirus (tswv), the type-member, also causes mild infection on its main insect vector, frankliniella occidentalis. herein, we identified an f. occidentalis putative transcription factor (fotf) that binds to the tswv rna-dependent rna polymerase and to viral rna. using in vitro rna synthesis assays, we show that addition of purified fotf improves viral replication, but n ... | 2005 | 15657123 |
| purification and serological analyses of tospoviral nucleocapsid proteins expressed by zucchini yellow mosaic virus vector in squash. | a plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus (zymv) was used to express the nucleocapsid proteins (nps) of tospoviruses in planta. the open reading frames (orfs) of nps of different serogroups of tospoviruses, including tomato spotted wilt virus, impatiens necrotic spot virus, watermelon silver mottle virus, peanut bud necrosis virus, and watermelon bud necrosis virus (wbnv), were in frame inserted in between the p1 and hc-pro genes of the zymv ... | 2005 | 15992936 |
| phage display-selected single-chain antibodies confer high levels of resistance against tomato spotted wilt virus. | rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. high cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were e ... | 2005 | 15958689 |
| host-associated genetic differentiation in thrips tabaci (insecta; thysanoptera), as determined from mtdna sequence data. | we tested for host-associated genetic differentiation in 22 populations of thrips tabaci collected from tobacco and leek, respectively. clustering analyses and haplotype networks based on sequence variation at a fragment of the mitochondrial cytochrome oxidase i gene yielded three major evolutionary lineages; two were clearly associated with leek and the third with tobacco. these genetic findings corroborated recent experimental observations on the heterogeneity of t. tabaci populations with reg ... | 2004 | 15241445 |
| trapping of tomato yellow leaf curl virus (tylcv) and other plant viruses with a groel homologue from the whitefly bemisia tabaci. | to avoid destruction in the haemolymph of their vector, many plant circulative viruses interact with groel homologues produced by insect endosymbiotic bacteria. we have exploited this phenomenon to devise tools allowing trapping of plant viruses by either groel purified from the whitefly bemisia tabaci or by whitefly groel over-expressed in e. coli. pcr tubes or 96-well plates coated with a groel preparation were incubated with cleared sap of virus infected plant leaves or insect vectors. groel- ... | 2004 | 15290375 |
| tobacco plants respond to the constitutive expression of the tospovirus movement protein ns(m) with a heat-reversible sealing of plasmodesmata that impairs development. | viral infection often results in typical symptoms, the biological background of which has remained elusive. we show that constitutive expression of the nsm viral movement protein (mp) of tomato spotted wilt virus in nicotiana tabacum is sufficient to induce severe, infection-like symptoms, including pronounced deficiencies in root and shoot development. leaves failed to expand and were arranged in a rosette due to the absence of internode elongation. following the sink-source transition they acc ... | 2005 | 16115066 |
| management of spotted wilt vectored by frankliniella fusca (thysanoptera: thripidae) in virginia market-type peanut. | field tests were conducted during 2001 and 2002 in northeastern north carolina to evaluate the impact of cultural practices and in-furrow insecticides on the incidence of tomato spotted wilt virus (genus tospovirus, family bunyaviridae, tswv), which is transmitted to peanut, arachis hypogaea l., primarily by tobacco thrips, frankliniella fusca hinds (thysanoptera: thripidae). treatments included in row plant populations of 7, 13, and 17 plants per meter; the virginia market-type 'nc v-11' and 'p ... | 2005 | 16334308 |
| host plant, temperature, and photoperiod effects on ovipositional preference of frankliniella occidentalis and frankliniella fusca (thysanoptera: thripidae). | host plant effects of tomato, lycopersicon esculentum mill., and chickweed, stellaria media (l.) vill., foliage infected and uninfected with tomato spotted wilt virus (family bunyaviridae, genus tospovirus, tswv) on the ovipositional preferences of western flower thrips, frankliniella occidentalis (pergande), and tobacco thrips, frankliniella fusca (hinds), were investigated for whole plants in the greenhouse. in addition, the preference for leaf disks from the same host plants was investigated ... | 2005 | 16539139 |
| viral suppressors of rna interference impair rna silencing induced by a semliki forest virus replicon in tick cells. | it was recently shown that infection of ise6 tick cells by a recombinant semliki forest virus (sfv) expressing a heterologous gene induced small interfering rnas (sirnas) and silencing of the gene. to gain information on rna interference (rnai) in ticks, three known viral inhibitors that act in different ways, the ns1 protein of influenza virus, nss of tospovirus tomato spotted wilt virus and hc-pro of zucchini yellow mosaic virus were expressed and investigated to determine if they antagonize i ... | 2006 | 16760400 |
| at-4/1, an interactor of the tomato spotted wilt virus movement protein, belongs to a new family of plant proteins capable of directed intra- and intercellular trafficking. | the tomato spotted wilt virus (tswv) encoded nsm movement protein facilitates cell-to-cell spread of the viral genome through structurally modified plasmodesmata. nsm has been utilized as bait in yeast two-hybrid interaction trap screenings. as a result, a protein of unknown function, called at-4/1, was isolated from an arabidopsis thaliana gal4 activation domain-tagged cdna library. using polyclonal antibodies against bacterially expressed at-4/1, western blot analysis of protein extracts isola ... | 2006 | 16903353 |
| relationship between esterase activity and acrinathrin and methiocarb resistance in field populations of western flower thrips, frankliniella occidentalis. | the western flower thrips, frankliniella occidentalis (pergande), is a serious pest in the south-east of spain owing to its direct feeding on crops, transmission of the tomato spotted wilt virus and its very high level of resistance to insecticides. mechanisms of resistance were examined using field populations of f. occidentalis with different susceptibilities to acrinathrin, methiocarb (selective insecticides), endosulfan, metamidophos and deltamethrin (broad-spectrum insecticides). esterase a ... | 2006 | 16929461 |
| tomato spotted wilt virus gc and n proteins interact in vivo. | tomato spotted wilt virus (tswv) virions consist of a nucleocapsid core surrounded by a membrane containing glycoproteins gn and gc. to unravel the protein interactions involved in the membrane acquisition of rnps, tswv nucleocapsid protein (n), gn and gc were expressed and analyzed in bhk21 cells. upon coexpression of gn, gc and n, a partial colocalization of n with both glycoproteins was observed in the golgi region. in contrast, upon coexpression of gc and n in the absence of gn, both protein ... | 2007 | 16963098 |
| matrix attachment regions increase the efficiency and stability of rna-mediated resistance to tomato spotted wilt virus in transgenic tobacco. | matrix attachment regions (mars) are dna elements that can increase and stabilize transgene expression. we investigated the effect of the rb7 mar on transgenic virus resistance. constructs for resistance to tomato spotted wilt virus (tswv) with and without flanking rb7 mars were used to transform tobacco and produce homozygous lines. the population with the mar construct had a significantly higher percentage of tswv resistant plants in the r1 generation than the nonmar population. each resistant ... | 2005 | 16022390 |
| evidence for plant viruses in the region of argentina islands, antarctica. | this work focused on the assessment of plant virus occurrence among primitive and higher plants in the antarctic region. sampling occurred during two seasons (2004/5 and 2005/6) at the ukrainian antarctic station 'academician vernadskiy' positioned on argentina islands. collected plant samples of four moss genera (polytrichum, plagiatecium, sanionia and barbilophozia) and one higher monocot plant species, deschampsia antarctica, were further subjected to enzyme-linked immunosorbent assay to test ... | 2007 | 17328120 |
| the cytoplasmic domain of tomato spotted wilt virus gn glycoprotein is required for golgi localisation and interaction with gc. | envelopment of tomato spotted wilt virus nucleocapsids occurs at the golgi stacks of infected cells. this is also the place where the two membrane glycoproteins gn and gc accumulate upon coexpression. the required golgi retention signal has previously been demonstrated to reside within gn. using a series of truncated gn proteins, the golgi retention signal was mapped to a stretch of 10 amino acids on this protein's cytoplasmic tail, 20 residues downstream the transmembrane domain. studies on the ... | 2007 | 17331557 |
| the tubule-forming nsm protein from tomato spotted wilt virus complements cell-to-cell and long-distance movement of tobacco mosaic virus hybrids. | a florida isolate of tomato spotted wilt virus (tswv) was able to complement cell-to-cell movement of a movement-defective tobacco mosaic virus (tmv) vector expressing the jellyfish green fluorescent protein (gfp). to test for complementation of movement in the absence of other tswv proteins, the open reading frame for the nsm protein was expressed from tmv constructs encoding only the tmv replicase proteins. nsm was expressed from either the coat protein or movement protein subgenomic promoter, ... | 2005 | 16112159 |
| characterization of the large (l) rna of peanut bud necrosis tospovirus. | the nucleocapsids purified from peanut plants systemically infected with peanut bud necrosis virus (pbnv), a member of the genus tospovirus, contained both viral(v) and viral complementary(vc) sense l rnas. defective forms of l rna containing 'core polymerase region' were observed. the full length l rna of pbnv was sequenced using overlapping cdna clones. the 8911 nucleotide l rna contains a single open reading frame (orf) in the vc strand, and encodes a protein of 330 kda. at the 5' and 3' term ... | 1998 | 9930194 |
| interrelationship of phytoalexin production and disease resistance in selected peanut genotypes. | in peanuts, a mechanism of resistance to fungal infection is reportedly due to the synthesis of stilbene phytoalexins, which are antibiotic, low molecular weight metabolites. the phytoalexin-associated response of different peanut genotypes to exogenous invasion in the field has not been investigated and may be useful for breeding resistant peanut cultivars. five peanut genotypes, georgia green, tifton 8, c-99r, gk-7 high oleic, and marc i, which differ in resistance to major peanut diseases, we ... | 2007 | 17326657 |
| high incidence of tobacco streak virus in the tobacco fields of iran. | currently the northern provinces of iran (mazandaran, golestan and gilan) are the main tobacco growing regions in the country and this crop has an special importance in the national economy. three tobacco types including flue-cured (mainly coker 347 and some virginia e1), burley (burley 21) and oriental (basma 178-2) are presently grown in these regions. epidemics of viral diseases have occurred during the recent years in many tobacco fields in these areas. the quality of tobacco products which ... | 2006 | 17390881 |
| impact of early-season thrips management on reducing the risks of spotted wilt virus and suppressing aphid populations in flue-cured tobacco. | the influence of tray drench (td) treatments, with and without foliar applications of the plant activator acibenzolar-s-methyl (actigard), was examined in replicated field plots in 2000--2002. td treatments of actigard, imidacloprid (admire), and these two products combined had little effect on seasonal mean thrips populations; however, thrips densities were lower in the admire-treated plots at 4 and 5 wk after transplanting. actigard and admire td treatments significantly reduced the seasonal i ... | 2005 | 15765674 |
| occurrence of viruses infecting pea in iran. | a survey was conducted to determine the incidence of alfalfa mosaic virus (amv), bean yellow mosaic virus (bymv), broad bean wilt virus-1 (bbwv), pea leafroll virus (plrv), pea enation mosaic virus (pemv), pea seed borne mosaic virus (psbmv), potato virus x(pvx), tomato mosaic virus (tomv), tomato spotted wilt virus (tswv) on pea (pisum sativum) in iran. a total of 1276 random and 684 symptomatic pea samples were collected during the spring and summer of 2002-2004 in tehran province of iran, whe ... | 2006 | 17390891 |
| tomato spotted wilt virus s-segment mrnas have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif. | the tomato spotted wilt virus ambisense m- and s-rna segments contain an a/u-rich intergenic region predicted to form a stable hairpin structure. the site of transcription termination of s-segment encoded n and nss mrnas synthesised in an in vitro transcription system was roughly mapped to the 3'-end of the intergenic hairpin, i.e. position 1568-1574 for n and position 1852-1839 for nss, as determined by rt-pcr cloning and size estimation on northern blots. this suggests that these viral transcr ... | 2005 | 15845263 |
| a rapid and efficient inoculation method for tomato spotted wilt tospovirus. | a rapid and efficient method of inoculation for tomato spotted wilt tospovirus (tswv) was achieved by applying the inoculum with a device consisting of a spray gun, an atomizer and a co2-powered sprayer. the inoculum contained infected leaf sap prepared in 0.1m phosphate buffer, ph 7.0, 0.2% sodium sulfite and 0.01 m 2-mercaptoethanol (1g: 10 ml) and 1% each of celite 545 and carborundum 320 grit. the spray application of chilled inoculum at the rate of 1.1 ml/plant and at an air pressure of 4.1 ... | 2008 | 18272238 |
| the n protein of tomato spotted wilt virus (tswv) is associated with the induction of programmed cell death (pcd) in capsicum chinense plants, a hypersensitive host to tswv infection. | in sweet pepper, the tsw gene, originally described in capsicum chinense, has been widely used as an efficient gene for inducing a hypersensitivity response (hr) derived tomato spotted wilt virus (tswv) resistance. since previously reported studies suggested that the tswv-s rna mutation(s) are associated with the breakdown of tsw mediated tswv resistance in peppers, the tswv genes n (structural nucleocapsid protein) and ns(s) (non-structural silencing suppressor protein) were cloned into a potat ... | 2008 | 18722487 |
| tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementarity to the viral template. | transcription of segmented negative-strand rna viruses is initiated by cap snatching: a host mrna is cleaved generally at 10-20 nt from its 5' capped end and the resulting capped leader used to prime viral transcription. for tomato spotted wilt virus (tswv), type species of the plant-infecting tospovirus genus within the bunyaviridae, cap donors were previously shown to require a single base complementarity to the ultimate or penultimate viral template sequence. more recently, the occurrence in ... | 2005 | 15823611 |
| evidence that the nonstructural protein of tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the tsw gene. | all known pepper cultivars resistant to tomato spotted wilt virus (tswv) possess a single dominant resistance gene, tsw. recently, naturally occurring resistance-breaking (rb) tswv strains have been identified, causing major concerns. we used a collection of such strains to identify the specific genetic determinant that allows the virus to overcome the tsw gene in capsicum spp. a reverse genetic approach is still not feasible for tswv; therefore, we analyzed reassortants between wild-type (wt) a ... | 2007 | 17506332 |
| the nucleoprotein of tomato spotted wilt virus as protein tag for easy purification and enhanced production of recombinant proteins in plants. | upon infection, tomato spotted wilt virus (tswv) forms ribonucleoprotein particles (rnps) that consist of nucleoprotein (n) and viral rna. these aggregates result from the homopolymerization of the n protein, and are highly stable in plant cells. these properties feature the n protein as a potentially useful protein fusion partner. to evaluate this potential, the n protein was fused to the aequorea victoria green fluorescent protein (gfp), either at the amino or carboxy terminus, and expressed i ... | 2007 | 17532227 |
| dynamics of tomato spotted wilt virus replication in the alimentary canal of two thrips species. | abstract transmission of tomato spotted wilt virus (tswv) is dependent on virus uptake in the midgut prior to virus movement to the salivary glands. replication of tswv in the alimentary canal of tobacco thrips (tt, frankliniella fusca) and western flower thrips (wft, f. occidentalis) was investigated by immunolocalization of the nonstructural protein (nss) encoded by the small rna of tswv and fluorescence microscopy. analysis of cohorts during development from larva to adults following virus ac ... | 2002 | 18943268 |
| biological and molecular analyses of the acibenzolar-s-methyl-induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus. | tomato spotted wilt virus (tswv) is an economically important virus of flue-cured tobacco. activation of systemic acquired resistance (sar) by acibenzolar-s-methyl (asm) in flue-cured tobacco was studied under greenhouse conditions by challenge inoculation with a severe isolate of tswv. asm restricted virus replication and movement, and as a result reduced systemic infection. activation of resistance was observed within 2 days after treatment with asm and a high level of resistance was observed ... | 2008 | 18943196 |
| field evaluation of tomato hybrids engineered with tomato spotted wilt virus sequences for virus resistance, agronomic performance, and pollen-mediated transgene flow. | abstract tomato hybrids obtained from homozygous progeny of line 30-4, engineered for tomato spotted wilt virus (tswv) resistance, were tested under field conditions in two locations with their corresponding nontransgenic hybrids. no transgenic hybrid became infected, but 33 to 50% of plants of each nontransgenic hybrid became infected with a severe reduction of marketable fruit production. the transgenic hybrids conformed to the standard agronomic characteristics of the corresponding nontransge ... | 2005 | 18943013 |
| a soluble form of the tomato spotted wilt virus (tswv) glycoprotein g(n) (g(n)-s) inhibits transmission of tswv by frankliniella occidentalis. | tomato spotted wilt virus (tswv) is an economically important virus that is transmitted in a persistent propagative manner by its thrips vector, frankliniella occidentalis. previously, we found that a soluble form of the envelope glycoprotein g(n) (g(n)-s) specifically bound thrips midguts and reduced the amount of detectable virus inside midgut tissues. the aim of this research was to (i) determine if g(n)-s alters tswv transmission by thrips and, if so, (ii) determine the duration of this effe ... | 2008 | 18943237 |
| completion of the genome sequence of watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. | abstract the nucleotide sequence of the l rna of watermelon silver mottle virus (wsmov) was determined. combined with the previous work on m and s rnas, the whole genomic sequence of this member of the genus tospovirus was completed. the l rna is 8,917 nucleotides in length, with one large open reading frame encoding a translation product of 2,878 amino acids (331.8 kda) on the viral complementary strand. the l protein shares amino acid identities of only 44.3 and 46.5% with tomato spotted wilt ... | 2001 | 18943848 |
| tomato spotted wilt virus infection improves host suitability for its vector frankliniella occidentalis. | abstract the effect of tomato spotted wilt virus (tswv) infection on plant attractiveness for the western flower thrips (frankliniella occidentalis) was studied. significantly more thrips were recovered on infected than were recovered on noninfected pepper (capsicum annuum) plants in different preference tests. in addition, more offspring were produced on the virus-infected pepper plants, and this effect also was found for tswv-infected datura stramonium. thrips behavior was minimally influenced ... | 2004 | 18943902 |
| interaction between tomato spotted wilt virus n protein monomers involves nonelectrostatic forces governed by multiple distinct regions in the primary structure. | abstract the ambisense rna genome of tomato spotted wilt virus (tswv) isby interaction with numerous copies of the virus encoded nucleocapsid (n) protein to form a subvirion structure called a ribonucleo-protein (rnp). rnps are central to the viral replication cycle because they, and not free viral rna, serve as templates for viral gene expression and genome replication. n protein monomers bind to viral rna molecules in a cooperative manner. we have examined regions of the n protein that are inv ... | 2004 | 18943909 |
| replication of tomato spotted wilt virus after ingestion by adult thrips setosus is restricted to midgut epithelial cells. | abstract if acquisition access feeding (aaf) is first given after adult eclosion, none of the nine thrips species able to serve as tospovirus vectors can become infective. the previous cellular investigations of this phenomenon, carried out only in frankliniella occidentalis, suggested that infectivity was prevented because the type member of the tospoviruses, tomato spotted wilt virus (tswv), was unable to enter the midgut of adult thrips. the present study extends a cellular view of tospovirus ... | 2001 | 18943329 |
| a predictive model for spotted wilt epidemics in peanut based on local weather conditions and the tomato spotted wilt virus risk index. | tomato spotted wilt virus (tswv), a member of the genus tospovirus (family bunyaviridae), is an important plant virus that causes severe damage to peanut (arachis hypogaea) in the southeastern united states. disease severity has been extremely variable in individual fields in georgia, due to several factors including variability in weather patterns. a tswv risk index has been developed by the university of georgia to aid peanut growers with the assessment and avoidance of high risk situations. t ... | 2008 | 18943452 |
| first report of occurrence of two viruses on pea field in iran. | an intensive survey was conducted to identify virus diseases affecting pea crops in tehran province of iran. a total of 270 pea samples were collected randomly from pea fields. samples were tested by double antibody sandwich enzyme linked immunosorbent assay (das-elisa) using polyclonal antisera prepared against psbmv (as-0129, dsmz, braunschweig, germany) and tswv (as-0580, dsmz, braunschweig, germany). virus disease incidence in pea samples was followed by psbmv (33%) tswv (24.4%) and psbmv+ts ... | 2005 | 16637207 |
| a one-step reverse transcription-polymerase chain reaction system for the simultaneous detection and identification of multiple tospovirus infections. | abstract a one-step reverse transcription-polymerase chain reaction (rt-pcr) method has been developed for the simultaneous detection and identification of multiple tospoviruses that infect plants. the rt-pcr system is composed of six primers in a single tube: a universal degenerate primer and five virus species-specific primers. amplifications resulted in an 848-bp pcr product for watermelon silver mottle virus, 709-bp for tomato spotted wilt virus, 589-bp for impatiens necrotic spot virus, 511 ... | 2005 | 18943986 |
| synergistic interaction between tomato chlorosis virus and tomato spotted wilt virus results in breakdown of resistance in tomato. | abstract multiple viral infections frequently are found in single plants of cultivated and wild hosts in nature, with unpredictable pathological consequences. synergistic reactions were observed in mixed infections in tomato plants doubly infected with the positive-sense and phloem-limited single-stranded rna (ssrna) crinivirus tomato chlorosis virus (tocv) and the negative-sense ssrna tospovirus tomato spotted wilt virus (tswv). synergism in a tomato cultivar susceptible to both viruses resulte ... | 2006 | 18943964 |
| overwintering of frankliniella fusca (thysanoptera: thripidae) on winter annual weeds infected with tomato spotted wilt virus and patterns of virus movement between susceptible weed hosts. | abstract overwintering of tobacco thrips, frankliniella fusca, was investigated on common winter annual host plants infected with tomato spotted wilt virus (tswv). populations of tobacco thrips produced on tswv-infected plants did not differ from those produced on healthy plants, whereas populations varied greatly among host plant species. the mean per plant populations of f. fusca averaged 401, 162, and 10 thrips per plant on stellaria media, scleranthus annuus, and sonchus asper, respectively, ... | 2001 | 18944235 |
| serological and molecular characterization of peanut chlorotic fan-spot virus, a new species of the genus tospovirus. | abstract to clarify the serological relationship of peanut chlorotic fan-spot virus (pcfv) with other tospoviruses, antisera were produced against the nucleocapsid (n) proteins of this virus and tospoviruses from four serogroups including tomato spotted wilt virus (tswv), impatiens necrotic spot virus (insv), groundnut ringspot virus (grsv), and watermelon silver mottle virus (wsmov). in immunodiffusion tests, the antisera only reacted with their homologous antigens. similar results were noticed ... | 2001 | 18944231 |
| role of insecticides in reducing thrips injury to plants and incidence of tomato spotted wilt virus in virginia market-type peanut. | tomato spotted wilt virus (family bunyaviridae, genus tospovirus, tswv), transmitted by many thrips species, is a devastating pathogen of peanut, arachis hypogaea l. tswv has become a serious problem in the virginia/carolina peanut-growing region of the united states. during 2002, tswv was present in 47% of the north carolina hectarage and caused a 5% yield reduction in virginia. factors influencing levels of tswv in runner market-type peanut cultivars, which are primarily grown in alabama, flor ... | 2007 | 17849876 |
| the efficiency by which thrips tabaci populations transmit tomato spotted wilt virus depends on their host preference and reproductive strategy. | abstract arrhenotokous and thelytokous populations of thrips tabaci from tobacco or leek plants were evaluated for their ability to transmit tomato spotted wilt virus (tswv) and for their host preference. transmission efficiencies were comparatively studied using leaf disks of petunia hybrida, datura stramonium, and nicotiana tabacum cv. basmas. adults of arrhenotokous populations collected on infected tobacco plants in the field were efficient transmitters (up to 48.5% transmission) and remaine ... | 2002 | 18944256 |
| acquisition of tomato spotted wilt virus by adults of two thrips species. | abstract only larval thrips that acquire tomato spotted wilt virus (tswv), or adults derived from such larvae, transmit the virus. nonviruliferous adults can ingest virus particles while feeding on tswv-infected plants, but such adult thrips have not been shown to transmit tswv. immunofluorescence microscopy was used to show that thrips 1, 5, 10, and 20 days after adult emergence (dae) fed on tswv-infected plants acquired tswv with virus replication and accumulation occurring in both epithelial ... | 2004 | 18944108 |
| thrips resistance in pepper and its consequences for the acquisition and inoculation of tomato spotted wilt virus by the western flower thrips. | abstract different levels of thrips resistance were found in seven capsicum accessions. based on the level of feeding damage, host preference, and host suitability for reproduction, a thrips susceptible and a resistant accession were selected to study their performance as tomato spotted wilt virus (tswv) sources and targets during thrips-mediated virus transmission. vector resistance did not affect the virus acquisition efficiency in a broad range of acquisition access periods. inoculation effic ... | 2003 | 18944162 |
| herbivore benefits from vectoring plant virus through reduction of period of vulnerability to predation. | herbivores can profit from vectoring plant pathogens because the induced defence of plants against pathogens sometimes interferes with the induced defence of plants against herbivores. plants can also defend themselves indirectly by the action of the natural enemies of the herbivores. it is unknown whether the defence against pathogens induced in the plant also interferes with the indirect defence against herbivores mediated via the third trophic level. we previously showed that infection of pla ... | 2008 | 18392858 |
| the glycoprotein gene of chrysanthemum stem necrosis virus and zucchini lethal chlorosis virus and molecular relationship with other tospoviruses. | two tospoviruses, chrysanthemum stem necrosis virus (csnv) and zucchini lethal chlorosis virus (zlcv), cause economical losses in several ornamental and vegetable crops in brazil. the nucleocapsid gene and movement protein sequences had already been reported for both viruses, though the glycoprotein precursor gene sequence was not available. in this study, cdna fragments (ca. 4 kb) of the m rna 3' portion of csnv (isolate chry-1) and zlcv (isolate 1038), including the complete glycoprotein precu ... | 2007 | 17570049 |
| tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and golgi-derived pleomorphic membrane structures in plant cells. | tomato spotted wilt virus (tswv) particles are spherical and enveloped, an uncommon feature among plant infecting viruses. previous studies have shown that virus particle formation involves the enwrapment of ribonucleoproteins with viral glycoprotein containing golgi stacks. in this study, the localization and behaviour of the viral glycoproteins gn and gc were analysed, upon transient expression in plant protoplasts. when separately expressed, gc was solely observed in the endoplasmic reticulum ... | 2008 | 18632951 |
| leaf proteome analysis of transgenic plants expressing antiviral antibodies. | the expression of exogenous antibodies in plant is an effective strategy to confer protection against viral infection or to produce molecules with pharmaceutical interest. however, the acceptance of the transgenic technology to obtain self-protecting plants depends on the assessment of their substantial equivalence compared to non-modified crops with an established history of safe use. in fact, the possibility exists that the introduction of transgenes in plants may alter expression of endogenou ... | 2009 | 19099506 |
| virus diseases in the tobacco fields of guilan and western azerbaijan provinces of iran. | tobacco (nicotiana tabacum l.) is one of the important industrial plants in iran. viruses as an important group of plant pathogens cause many losses on the quality and quantity of tobacco crop. there was few information on the types of plant viruses infecting the tobacco fields of guilan and almost no information for western azerbaijan province. during 2005-2007, leaf samples were taken from symptomatic plants in the growing areas of these two provinces. the observed symptoms on plants in the fi ... | 2008 | 19226768 |
| tomato spotted wilt virus nucleocapsid protein interacts with both viral glycoproteins gn and gc in planta. | recently, the tomato spotted wilt virus (tswv) gn and gc glycoproteins were shown to induce the formation of (pseudo-) circular and pleomorphic membrane structures upon transient expression in plant cells. furthermore, when singly expressed, gc retains in the er, while gn is able to further migrate to the golgi. upon co-expression, gn rescues gc and co-migrates to the golgi complex. here, we have studied the behavior of the glycoproteins in the presence of the viral nucleocapsid (n) protein and ... | 2009 | 18973913 |
| variation in tomato spotted wilt virus titer in frankliniella occidentalis and its association with frequency of transmission. | tomato spotted wilt virus (tswv) is transmitted in a persistent propagative manner by frankliniella occidentalis, the western flower thrips. while it is well established that vector competence depends on tswv acquisition by young larvae and virus replication within the insect, the biological factors associated with frequency of transmission have not been well characterized. we hypothesized that the number of transmission events by a single adult thrips is determined, in part, by the amount of vi ... | 2009 | 19271982 |
| investigating recombinant protein exudation from roots of transgenic tobacco. | it is widely acknowledged that plant-made pharmaceuticals (pmps) offer numerous benefits, including inexpensive production, biological safety and the facility for production at agricultural scale. at the same time, it is important to minimize any potential risk associated with this new technology, including the potential release of bioactive proteins into the environment. to address this issue, we studied transgenic nicotiana benthamiana and nicotiana tabacum plants expressing two recombinant si ... | 2008 | 19081009 |
| effects of temperature and host on the generation of tomato spotted wilt virus defective interfering rnas. | abstract the generation of defective interfering (di) rna molecules of tomato spotted wilt tospovirus (tswv) was studied by serially passaging in-ocula from plant to plant under different controlled conditions. di rnas were generated at higher rates in plants at 16 degrees c than in plants incubated at higher temperatures. another factor promoting the tswv di rna generation was the use of high virus concentrations in the inocula. the solanaceous species capsicum annuum, datura stramonium, lycope ... | 1997 | 18945014 |
| requirements for er-arrest and sequential exit to the golgi of tomato spotted wilt virus glycoproteins. | the envelope glycoproteins gn and gc are major determinants in the assembly of tomato spotted wilt virus (tswv) particles at the golgi complex. in this article, the er-arrest of singly expressed gc and the transport of both glycoproteins to the golgi upon coexpression have been analyzed.while preliminary results suggest that the arrest of gc at the er (endoplasmic reticulum) did not appear to result from improper folding, transient expression of chimeric gc, in which the transmembrane domain (tm ... | 2009 | 19302268 |
| bell and banana pepper exhibit mature-plant resistance to tomato spotted wilt tospovirus transmitted by frankliniella fusca (thysanoptera: thripidae). | tomato spotted wilt virus (family bunyaviridae, genus tospovirus, tswv) causes annual economic losses in pepper, capsicum annuum l., across the southern united states and is transmitted by several species of thrips, including the tobacco thrips, frankliniella fusca (hinds). reduced virus transmission and symptom severity as plant age increases is known as mature-plant resistance. tswv transmission to pepper plants was examined in three and four age classes in field and greenhouse trials, respect ... | 2009 | 19253614 |
| comparative analysis of expression profiles in shoots and roots of tomato systemically infected by tomato spotted wilt virus reveals organ-specific transcriptional responses. | tomato (solanum lycopersicon), a model species for the family solanaceae, is severely affected by tomato spotted wilt virus (tswv) worldwide. to elucidate the systemic transcriptional response of plants to tswv infection, microarray experiments were performed on tomato. parallel analysis of both shoots and roots revealed organ-specific responses, although the virus was present in similar concentration. in the shoots, genes related to defense and to signal transduction were induced, while there w ... | 2009 | 19888816 |
| restricted spread of tomato spotted wilt virus in thrips-resistant pepper. | abstract spread of tomato spotted wilt virus (tswv) and population development of its vector frankliniella occidentalis were studied on the pepper accessions cpro-1 and pikante reuzen, which are resistant and susceptible to thrips, respectively. viruliferous thrips were released on plants of each accession (nonchoice tests) or on plants in a 1:1 mixture of both accessions (choice tests) in small cages containing 8 or 16 plants. significantly fewer cpro-1 plants became infected in the primary inf ... | 2003 | 18944320 |
| identification of domains of the tomato spotted wilt virus nsm protein involved in tubule formation, movement and symptomatology. | deletion and alanine-substitution mutants of the tomato spotted wilt virus nsm protein were generated to identify domains involved in tubule formation, movement and symptomatology using a heterologous tobacco mosaic virus expression system. two regions of nsm, g(19)-s(159) and g(209)-v(283), were required for both tubule formation in protoplasts and cell-to-cell movement in plants, indicating a correlation between these activities. three amino acid groups, d(154), eykk(205-208) and eeeee(284-288 ... | 2009 | 19481775 |
| comparative post-harvest behaviour of traditional and virus-resistant muchamiel tomatoes. | nowadays, organoleptic quality is the primary objective for almost all tomato breeding programmes. in this study, post-harvest behaviour of a breeding line with genetic resistance to important viruses (tomato mosaic virus, tomato spotted wilt virus and tomato yellow leaf curl virus) has been compared with the original traditional landrace (muchamiel). the breeding line has been obtained by backcrossing, introgressing three resistance genes but aiming to keep the quality characteristics of the tr ... | 2010 | 20355147 |
| analysis of gene expression profiles in leaf tissues of cultivated peanuts and development of est-ssr markers and gene discovery. | peanut is vulnerable to a range of foliar diseases such as spotted wilt caused by tomato spotted wilt virus (tswv), early (cercospora arachidicola) and late (cercosporidium personatum) leaf spots, southern stem rot (sclerotium rolfsii), and sclerotinia blight (sclerotinia minor). in this study, we report the generation of 17,376 peanut expressed sequence tags (ests) from leaf tissues of a peanut cultivar (tifrunner, resistant to tswv and leaf spots) and a breeding line (gt-c20, susceptible to ts ... | 2009 | 19584933 |
| application of phage display in selecting tomato spotted wilt virus-specific single-chain antibodies (scfvs) for sensitive diagnosis in elisa. | abstract a panel of recombinant single-chain antibodies (scfvs) against structural proteins of tomato spotted wilt virus (tswv) was retrieved from a human combinatorial scfv antibody library using the novel phage display technique. after subcloning the encoding dna sequences in the expression vector pskap/s, which allowed the scfvs to be expressed as alkaline phosphatase fusion proteins, 17 different scfv antibodies were obtained. of these, 12 scfvs were directed against the nucleoprotein (n) an ... | 2000 | 18944607 |
| increase of tospoviral diversity in brazil with the identification of two new tospovirus species, one from chrysanthemum and one from zucchini. | abstract during a survey conducted in several different regions of brazil, two unique tospoviruses were isolated and characterized, one from chrysanthemum and the other from zucchini. the chrysanthemum virus displayed a broad host range, whereas the virus from zucchini was restricted mainly to the family cucurbitaceae. double-antibody sandwich-enzyme-linked immunosorbent assay and western immunoblot analyses demonstrated that both viruses were serologically distinct from all reported tospovirus ... | 1999 | 18944712 |
| comparison of ambisense m rna of watermelon silver mottle virus with other tospoviruses. | abstract double-stranded genomic rnas (dsrnas) extracted from chenopodium quinoa infected with watermelon silver mottle virus (wsmv) were similar to those of tomato spotted wilt virus (tswv, serogroup i) and impatiens necrotic spot virus (insv, serogroup iii), except that the s dsrna of wsmv is 0.75 and 0.6 kbp longer than those of tswv and insv, respectively. the complete nucleotide sequence of the genomic m rna of wsmv was determined from cdna clones generated from separated m dsrna. the m rna ... | 1998 | 18944959 |