Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| the functional analysis of distinct tospovirus movement proteins (nsm) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species. | the lack of infectious tospovirus clones to address reverse genetic experiments has compromised the functional analysis of viral proteins. in the present study we have performed a functional analysis of the movement proteins (nsm) of four tospovirus species bean necrotic mosaic virus (benmv), chrysanthemum stem necrosis virus (csnv), tomato chlorotic spot virus (tcsv) and tomato spotted wilt virus (tswv), which differ biologically and molecularly, by using the alfalfa mosaic virus (amv) model sy ... | 2017 | 27697453 |
| genetic dissection of novel qtls for resistance to leaf spots and tomato spotted wilt virus in peanut (arachis hypogaea l.). | peanut is an important crop, economically and nutritiously, but high production cost is a serious challenge to peanut farmers as exemplified by chemical spray to control foliar diseases such as leaf spots and thrips, the vectors of tomato spotted wilt virus (tswv). the objective of this research was to map the quantitative trait loci (qtls) for resistance to leaf spots and tswv in one recombinant inbred line (ril) mapping population of "tifrunner × gt-c20" for identification of linked markers fo ... | 2017 | 28197153 |
| development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to tomato spotted wilt virus. | tospoviruses, the plant-infecting genus in the family bunyaviridae, are thrips borne and cause severe agricultural losses worldwide. based on the serological relationships of the structural nucleocapsid protein (np), the current tospoviruses are divided into six serogroups. the use of np-antisera is convenient for virus detection, but it is insufficient to identify virus species grouped in a serogroup due to the serological cross-reaction. alternatively, virus species can be identified by the n ... | 2017 | 28081705 |
| odor, not performance, dictates bemisia tabaci's selection between healthy and virus infected plants. | although, insect herbivores are generally thought to select hosts that favor the fitness of their progeny, this "mother-knows-best" hypothesis may be challenged by the presence of a plant virus. our previous study showed that the whitefly, bemisia tabaci, the obligate vector for transmitting tomato yellow leaf curl virus (tylcv), preferred to settle and oviposit on tylcv-infected rather than healthy host plant, datura stramonium. the performances of b. tabaci larvae and adults were indeed improv ... | 2017 | 28360861 |
| distribution of tomato spotted wilt virus in dahlia plants. | tomato spotted wilt virus (tswv) causes significant losses in the production of the ornamental plant dahlia variabilis in japan. the purpose of this study was to examine the distribution of tswv in dahlia plants and identify plant parts that can be used in the selection of tswv-free plants. the distribution of tswv was investigated using reverse transcriptional polymerase chain reaction (rt-pcr) and tissue blot immunoassay. the detection rate of tswv in latent infected compound leaves was the hi ... | 2017 | 28129432 |
| erratum to: complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato sw-5 resistance-breaking and pepper tsw resistance-breaking) from spain. | 2017 | 27981408 | |
| thrips developmental stage-specific transcriptome response to tomato spotted wilt virus during the virus infection cycle in frankliniella occidentalis, the primary vector. | tomato spotted wilt virus (tswv) is transmitted by frankliniella occidentalis in a circulative-propagative manner. little is known about thrips vector response to tswv during the infection process from larval acquisition to adult inoculation of plants. whole-body transcriptome response to virus infection was determined for first-instar larval, pre-pupal and adult thrips using rna-seq. tswv responsive genes were identified using preliminary sequence of a draft genome of f. occidentalis as a refer ... | 2017 | 27835811 |
| differential proteomics analysis of frankliniella occidentalis immune response after infection with tomato spotted wilt virus (tospovirus). | tomato spotted wilt virus (tswv) is mainly vectored by frankliniella occidentalis pergande, and it potentially activates the vector's immune response. however, molecular background of the altered immune response is not clearly understood. therefore, using a proteomic approach, we investigated the immune pathways that are activated in f. occidentalis larvae after 24 h exposure to tswv. two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2d-ief/sds/page) ... | 2017 | 27810283 |
| divergent evolution of multiple virus-resistance genes from a progenitor in capsicum spp. | plants have evolved hundreds of nucleotide-binding and leucine-rich domain proteins (nlrs) as potential intracellular immune receptors, but the evolutionary mechanism leading to the ability to recognize specific pathogen effectors is elusive. here, we cloned pvr4 (a potyvirus resistance gene in capsicum annuum) and tsw (a tomato spotted wilt virus resistance gene in capsicum chinense) via a genome-based approach using independent segregating populations. the genes both encode typical nlrs and ar ... | 2017 | 27612097 |
| development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species. | a luminex xtag-based assay for plant-infecting tospoviruses was developed. the test enables the detection of tospoviruses in general and the differentiation of the four important member species of this genus: tomato spotted wilt virus, impatiens necrotic spot virus, the proposed 'capsicum chlorosis virus' and watermelon silver mottle virus. the generic tospovirus primers used in this method are also applicable for detection of tospoviruses by basic rt-pcr. we also describe an economic alternativ ... | 2017 | 28190200 |
| an efficient and high fidelity method for amplification, cloning and sequencing of complete tospovirus genomic rna segments. | tospoviruses (genus tospovirus, family bunyaviridae) are responsible for major losses in an extensive range of crops worldwide. new species of these single-stranded, ambisense rna viruses regularly emerge and have been shown to maintain heterogeneous populations with individual isolates having quite variable biological and virulence characteristics. most tospovirus phylogenetic studies have focused on analysis of a single gene, most often the nucleocapsid protein gene. complete genomic rna segme ... | 2017 | 28082165 |
| transcriptome-wide identification of host genes targeted by tomato spotted wilt virus-derived small interfering rnas. | rna silencing mechanism functions as a major defense against invading viruses. the caveat in the rna silencing mechanism is that the effector small interfering rnas (sirnas) act on any rna transcripts with sequence complementarity irrespective of target's origin. a subset of highly expressed viral small interfering rnas (vsirnas) derived from the tomato spotted wilt virus (tswv; tospovirus: bunyaviridae) genome was analyzed for their propensity to downregulate the tomato transcriptome. a total o ... | 2017 | 28545854 |
| a single point mutation in tomato spotted wilt virus nss protein is sufficient to overcome tsw-gene-mediated resistance in pepper. | the nonstructural protein (nss) of tomato spotted wilt virus (tswv) was previously identified as an avirulence determinant for tsw-based resistance on pepper. the nss of wild-type (wt) and resistance-breaking (rb) tswv strains isolated in hungary had only two amino acid substitutions (104, 461). we have analysed the ability of the nss and their point mutant variants to trigger tsw-mediated hypersensitive responses and rna silencing suppressor (rss) activity in patch assays. we identified a singl ... | 2017 | 28631603 |
| three decades of managing tomato spotted wilt virus in peanut in southeastern united states. | southeastern states namely georgia, florida, and alabama produce two-thirds of the peanuts in the united states. thrips-transmitted tomato spotted wilt virus (tswv), which causes spotted wilt disease, has been a major impediment to peanut production for the past three decades. the cultivars grown in the 1980s were extremely susceptible to tswv. early yield losses extended to tens of millions of dollars each year (up to 100% loss in many fields). this situation led to the creation of an interdisc ... | 2017 | 28549856 |
| the nsm proteins of phylogenetically related tospoviruses trigger sw-5b-mediated resistance dissociated of their cell-to-cell movement function. | the cell-to-cell movement protein (nsm) of tomato spotted wilt virus (tswv) has been recently identified as the effector of the single dominant sw-5b resistance gene from tomato (solanum lycopersicum l.). although most tswv isolates shows a resistance-inducing (ri) phenotype, regular reports have appeared on the emergence of resistance-breaking (rb) isolates in tomato fields, and suggested a strong association with two point mutations (c118y and t120n) in the nsm protein. in this study the sw-5b ... | 2017 | 28754561 |
| towards area wide management of insect vectored viruses of tomatoes in the bowen district. | the bowen region of northern queensland is an important winter production area for tomatoes in australia. there are three economically important viruses in the region that affect tomato, tomato yellow leaf curl virus (tylcv), tomato spotted wilt virus (tswv) and potato leafroll virus (plrv), which are vectored by whiteflies, thrips and aphids, respectively. an area wide management approach is required to lower the primary inoculum throughout the district. to this end, we undertook investigations ... | 2017 | 28625668 |
| genome-wide discovery of microsatellite markers from diploid progenitor species, arachis duranensis and a. ipaensis, and their application in cultivated peanut (a. hypogaea). | despite several efforts in the last decade toward development of simple sequence repeat (ssr) markers in peanut, there is still a need for more markers for conducting different genetic and breeding studies. with the effort of the international peanut genome initiative, the availability of reference genome for both the diploid progenitors of cultivated peanut allowed us to identify 135,529 and 199,957 ssrs from the a (arachis duranensis) and b genomes (arachis ipaensis), respectively. genome sequ ... | 2017 | 28769940 |
| the asymmetric trimeric ring structure of the nucleocapsid protein of tospovirus. | tomato spotted wilt virus (tswv), belonging to the genus tospovirus of the family bunyaviridae, causes significant economic damage in several vegetables and ornamental plants worldwide. similar to that in all other negative-strand rna viruses, the nucleocapsid (n) protein plays very important roles in their viral life cycle. n proteins protect genomic rnas by encapsidation and form a viral ribonucleoprotein complex (vrnp) with some rna-dependent rna polymerases. here we show the crystal structur ... | 2017 | 28768868 |
| receiver operating characteristic curve analysis determines association of individual potato foliage volatiles with onion thrips preference, cultivar and plant age. | tomato spotted wilt virus (tswv) causes sporadic but serious disease in australian potato crops. tswv is naturally spread to potato by thrips of which thrips tabaci is the most important. prior studies indicated possible non-preference of potato cultivars to t. tabaci. select potato cultivars were assessed for non-preference to t. tabaci in paired and group choice trials. cultivars 'bismark', 'tasman' and 'king edward' were less preferred than 'atlantic', 'russet burbank' and 'shepody'. green le ... | 2017 | 28746359 |
| transcriptome changes associated with tomato spotted wilt virus infection in various life stages of its thrips vector, frankliniella fusca (hinds). | persistent propagative viruses maintain intricate interactions with their arthropod vectors. in this study, we investigated the transcriptome-level responses associated with a persistent propagative phytovirus infection in various life stages of its vector using an illumina hiseq sequencing platform. the pathosystem components included a tospovirus, tomato spotted wilt virus (tswv), its insect vector, frankliniella fusca (hinds), and a plant host, arachis hypogaea (l.). we assembled (de novo) re ... | 2017 | 28741996 |
| cell death triggering and effector recognition by sw-5 sd-cnl proteins from resistant and susceptible tomato isolines to tomato spotted wilt virus. | only a limited number of dominant resistance genes acting against plant viruses have been cloned, and further functional studies of these have been almost entirely limited to the resistance genes rx against potato virus x (pvx) and n against tobacco mosaic virus (tmv). recently, the cell-to-cell movement protein (nsm ) of tomato spotted wilt virus (tswv) has been identified as the avirulence determinant (avr) of sw-5b-mediated resistance, a dominant resistance gene which belongs to the class of ... | 2016 | 27271212 |
| manipulation of frankliniella occidentalis (thysanoptera: thripidae) by tomato spotted wilt virus (tospovirus) via the host plant nutrients to enhance its transmission and spread. | earlier studies have shown that tomato spotted wilt virus (tswv) influences the biology, performance, and behavioral patterns of its vector frankliniella occidentalis pergande. in this study, using capsicum annuum l. as the host plant, we aimed to determine the manipulation of f. occidentalis by tswv through switching of the diet (+ or -tswv) during vector's development. behavioral patterns, fitness, as well as vector performance were evaluated. the specific parameters investigated included long ... | 2016 | 27566527 |
| evaluation of alternatives to carbamate and organophosphate insecticides against thrips and tomato spotted wilt virus in peanut production. | thrips are important pests of peanut. they cause severe feeding injuries on peanut foliage in the early season. they also transmit tomato spotted wilt virus (tswv), which causes spotted wilt disease. at-plant insecticides and cultivars that exhibit field resistance to tswv are often used to manage thrips and spotted wilt disease. historically, peanut growers used the broad-spectrum insecticides aldicarb (irac class 1a; temik) and phorate (irac class 1b; thimet) for managing thrips and thereby re ... | 2016 | 26637534 |
| mapping quantitative trait loci of resistance to tomato spotted wilt virus and leaf spots in a recombinant inbred line population of peanut (arachis hypogaea l.) from sunoleic 97r and nc94022. | peanut is vulnerable to a range of diseases, such as tomato spotted wilt virus (tswv) and leaf spots which will cause significant yield loss. the most sustainable, economical and eco-friendly solution for managing peanut diseases is development of improved cultivars with high level of resistance. we developed a recombinant inbred line population from the cross between sunoleic 97r and nc94022, named as the s-population. an improved genetic linkage map was developed for the s-population with 248 ... | 2016 | 27427980 |
| seehabitat: a server on bioinformatics applications for tospoviruses and other species. | plant viruses are important limiting factors in agricultural productivity. tospovirus is one of the severe plant pathogens, causing damage to economically important food and ornamental crops worldwide through thrips as vectors. database application resources exclusively on this virus would help to design better control measures, which aren't available. seehabitat is a unique and exclusive web based server providing work bench to perform computational research on tospoviruses and its species. see ... | 2016 | 27354938 |
| antiviral rna silencing suppression activity of tomato spotted wilt virus nss protein. | in addition to regulating gene expression, rna silencing is an essential antiviral defense system in plants. triggered by double-stranded rna, silencing results in degradation or translational repression of target transcripts. viruses are inducers and targets of rna silencing. to condition susceptibility, most plant viruses encode silencing suppressors that interfere with this process, such as the tomato spotted wilt virus (tswv) nss protein. the mechanism by which nss suppresses rna silencing a ... | 2016 | 27323202 |
| resistance to tospoviruses in vegetable crops: epidemiological and molecular aspects. | during the past three decades, the economic impact of tospoviruses has increased, causing high yield losses in a variety of crops and ornamentals. owing to the difficulty in combating thrips vectors with insecticides, the best way to limit/prevent tospovirus-induced diseases involves a management strategy that includes virus resistance. this review briefly presents current tospovirus taxonomy, diversity, molecular biology, and cytopathology as an introduction to a more extensive description of t ... | 2016 | 27296139 |
| predictive models for tomato spotted wilt virus spread dynamics, considering frankliniella occidentalis specific life processes as influenced by the virus. | several models have been studied on predictive epidemics of arthropod vectored plant viruses in an attempt to bring understanding to the complex but specific relationship between the three cornered pathosystem (virus, vector and host plant), as well as their interactions with the environment. a large body of studies mainly focuses on weather based models as management tool for monitoring pests and diseases, with very few incorporating the contribution of vector's life processes in the disease dy ... | 2016 | 27159134 |
| sequence characterization, molecular phylogeny reconstruction and recombination analysis of the large rna of tomato spotted wilt virus (tospovirus: bunyaviridae) from the united states. | tomato spotted wilt virus (tswv; tospovirus: bunyaviridae) has been an economically important virus in the usa for over 30 years. however the complete sequence of only one tswv isolate pa01 characterized from pepper in pennsylvania is available. | 2016 | 27038777 |
| salivary gland morphology, tissue tropism and the progression of tospovirus infection in frankliniella occidentalis. | tomato spotted wilt virus (tswv) is transmitted by thrips in a propagative manner; however, progression of virus infection in the insect is not fully understood. the goal of this work was to study the morphology and infection of thrips salivary glands. the primary salivary glands (psg) are complex, with three distinct regions that may have unique functions. analysis of tswv progression in thrips revealed the presence of viral proteins in the foregut, midgut, ligaments, tubular salivary glands (t ... | 2016 | 26999025 |
| evaluation and identification of candidate genes for artificial microrna-mediated resistance to tomato spotted wilt virus. | tomato spotted wilt virus (tswv) is an economically important viral pathogen of a wide range of field and horticultural crops. we developed an artificial microrna (amirna) strategy against tswv, targeting the nucleoprotein (n) and silencing suppressor (nss) genes. the amirna constructs replaced the natural mirna in a shortened arabidopsis 173-nucleotide (nt) mir159a precursor backbone (athmir159a) without the stem base extending beyond the mir/mir* duplex. further, each amirna was modified to co ... | 2016 | 26454192 |
| comparison of small rna profiles in nicotiana benthamiana and solanum lycopersicum infected by polygonum ringspot tospovirus reveals host-specific responses to viral infection. | viral small rnas (vsrnas) are one of the key elements involved in rna silencing-based defense against viruses in plants. we analyzed the vsrna profiles in nicotiana benthamiana and solanum lycopersicum infected by polygonum ringspot virus (polrsv) (tospovirus, bunyaviridae). vsrnas were abundant in both hosts, but a different size profile was observed, with an abundance peak at 21 in n. benthamiana and at 22 nt in tomato. vsrnas mapping to the polrsv l genomic segment were under-represented in b ... | 2016 | 26432447 |
| genome-wide identification and expression profiling of tomato hsp20 gene family in response to biotic and abiotic stresses. | the hsp20 genes are involved in the response of plants to environment stresses including heat shock and also play a vital role in plant growth and development. they represent the most abundant small heat shock proteins (shsps) in plants, but little is known about this family in tomato (solanum lycopersicum), an important vegetable crop in the world. here, we characterized heat shock protein 20 (slhsp20) gene family in tomato through integration of gene structure, chromosome location, phylogeneti ... | 2016 | 27582749 |
| identification of major qtls underlying tomato spotted wilt virus resistance in peanut cultivar florida-ep(tm) '113'. | spotted wilt caused by tomato spotted wilt virus (tswv) is one of the major peanut (arachis hypogaea l.) diseases in the southeastern united states. occurrence, severity, and symptoms of spotted wilt disease are highly variable from season to season, making it difficult to efficiently evaluate breeding populations for resistance. molecular markers linked to spotted wilt resistance could overcome this problem and allow selection of resistant lines regardless of environmental conditions. florida-e ... | 2016 | 27600750 |
| host-specific accumulation and temperature effects on the generation of dimeric viral rna species derived from the s-rna of members of the tospovirus genus. | polygonum ringspot virus (polrsv) is a recently characterized tospovirus reported in italy. northern blot analyses of polrsv infections in nicotiana benthamiana and tomato plants showed that a viral rna species with nearly twice the length of the small genomic rna (s-rna) accumulated abundantly in the former host, but was not detected in the latter. additional assays confirmed that biogenesis of this novel rna species was common to all polrsv isolates tested and also to an isolate of tomato spot ... | 2016 | 27600541 |
| clustering and cellular distribution characteristics of virus particles of tomato spotted wilt virus and tomato zonate spot virus in different plant hosts. | tomato spotted wilt virus (tswv) and tomato zonate spot virus (tzsv) are the two dominant species of thrip-transmitted tospoviruses, cause significant losses in crop yield in yunnan and its neighboring provinces in china. tswv and tzsv belong to different serogroup of tospoviruses but induce similar symptoms in the same host plant species, which makes diagnostic difficult. we used different electron microscopy preparing methods to investigate clustering and cellular distribution of tswv and tzsv ... | 2016 | 26786326 |
| identification of three new isolates of tomato spotted wilt virus from different hosts in china: molecular diversity, phylogenetic and recombination analyses. | destructive diseases caused by tomato spotted wilt virus (tswv) have been reported associated with many important plants worldwide. recently, tswv was reported to infect different hosts in china. it is of value to clone tswv isolates from different hosts and examine diversity and evolution among different tswv isolates in china as well as worldwide. | 2016 | 26762153 |
| new mexico and the southwestern us are affected by a unique population of tomato spotted wilt virus (tswv) strains. | tomato spotted wilt virus (tswv) is an important pathogen of many ornamental, greenhouse and agronomic crops worldwide. tswv also causes sporadic problems in a number of crops in new mexico (nm). nucleocapsid gene sequences obtained from six different crop species across the state over four different years were used to characterize the nm tswv population. this analysis shows that nm is affected by a unique tswv population that is part of larger independent population present in the southwestern ... | 2016 | 26721573 |
| the genus tospovirus: emerging bunyaviruses that threaten food security. | the genus tospovirus is unique within the family bunyaviridae in that it is made up of viruses that infect plants. initially documented over 100 years ago, tospoviruses have become increasingly important worldwide since the 1980s due to the spread of the important insect vector frankliniella occidentalis and the discovery of new viruses. as a result, tospoviruses are now recognized globally as emerging agricultural diseases. tospoviruses and their vectors, thrips species in the order thysanopter ... | 2016 | 27578436 |
| effect of spinosad resistance on transmission of tomato spotted wilt virus by the western flower thrips (thysanoptera: thripidae). | tomato spotted wilt virus (tswv) is transmitted by frankliniella occidentalis (pergande) in a persistent-propagative manner. we previously observed significant results in terms of feeding behavior of spinosad-susceptible (ivf03) and -resistant (spin-r) strains of f. occidentalis using electrical penetration graph. tswv transmission by the two strains was compared in the present study. the results showed that the titer of tswv-n rna (a part of s rna of tswv and encoding the nucleocapsid protein) ... | 2016 | 26377766 |
| homology modeling and molecular dynamics provide structural insights into tospovirus nucleoprotein. | tospovirus is a plant-infecting genus within the family bunyaviridae, which also includes four animal-infecting genera: hantavirus, nairovirus, phlebovirus and orthobunyavirus. compared to these members, the structures of tospovirus proteins still are poorly understood. despite multiple studies have attempted to identify candidate n protein regions involved in rna binding and protein multimerization for tospovirus using yeast two-hybrid systems (y2hs) and site-directed mutagenesis, the tospoviru ... | 2016 | 28105914 |
| morphological and chemical characteristics of doubled haploids of flue-cured tobacco combining resistance to thielaviopsis basicola and tswv. | thielaviopsis basicola and tomato spotted wilt virus (tswv) are the most important problems in a moderate climate zone. previously obtained doubled haploids (dh) of f1 hybrids of the flue-cured line wgl3 resistant to th. basicola and the dark-cured line pw-834 carrying rtsw-al gene provided the research material. biological tests and scar markers linked with tswv were applied to confirm resistance of dh. lines combining resistance to tswv and th. basicola were evaluated for morphological and che ... | 2016 | 27162500 |
| salicylic acid is involved in the basal resistance of tomato plants to citrus exocortis viroid and tomato spotted wilt virus. | tomato plants expressing the nahg transgene, which prevents accumulation of endogenous salicylic acid (sa), were used to study the importance of the sa signalling pathway in basal defence against citrus exocortis viroid (cevd) or tomato spotted wilt virus (tswv). the lack of sa accumulation in the cevd- or tswv-infected nahg tomato plants led to an early and dramatic disease phenotype, as compared to that observed in the corresponding parental money maker. addition of acibenzolar-s-methyl, a ben ... | 2016 | 27893781 |
| identification of leonurus sibiricus as a weed reservoir for three pepper-infecting viruses. | in plant virus ecology, weeds are regarded as wild reservoirs of viruses and as potential sources for insect-mediated transmission of viruses. during field surveys in 2013-2014, three leonurus sibiricus plants showing virus-like symptoms were collected from pepper fields in daegu, seosan, and danyang in korea. molecular diagnosis assays showed that the collected l. sibiricus samples were infected with either tomato spotted wilt virus (tswv), pepper mild mottle virus (pmmov), or beet western yell ... | 2016 | 26889117 |
| development of reverse transcription thermostable helicase-dependent dna amplification for the detection of tomato spotted wilt virus. | a protocol for the reverse transcription-helicase-dependent amplification (rt-hda) of isothermal dna was developed for the detection of tomato spotted wilt virus (tswv). specific primers, which were based on the highly conserved region of the n gene sequence in tswv, were used for the amplification of virus's rna. the lod of rt-hda, reverse transcriptase-loop-mediated isothermal amplification (rt-lamp), and reverse transcriptase-polymerase chain reaction (rt-pcr) assays were conducted using 10-f ... | 2016 | 27538747 |
| the tomato spotted wilt virus genome is processed differentially in its plant host arachis hypogaea and its thrips vector frankliniella fusca. | thrips-transmitted tospoviruses are economically important viruses affecting a wide range of field and horticultural crops worldwide. tomato spotted wilt virus (tswv) is the type member of the tospovirus genus with a broad host range of more than 900 plant species. interactions between these viruses and their plant hosts and insect vectors via rnai pathways are likely a key determinant of pathogenicity. the current investigation, for the first time, compares biogenesis of small rnas between the ... | 2016 | 27656190 |
| simultaneous detection of tomato spotted wilt virus, dahlia mosaic virus and chrysanthemum stunt viroid by multiplex rt-pcr in dahlias and their distribution in japanese dahlias. | tomato spotted wilt virus (tswv), dahlia mosaic virus (dmv) and chrysanthemum stunt viroid (csvd) are economically important viruses and viroid that infect cultivated dahlias. prior to this investigation, no multiplex rt-pcr assay for the detection of dahlia virus and viroid infections existed. in this study, we report the development of a multiplex rt-pcr that simultaneously detects tswv, dmv and csvd infections in dahlias. in addition, a simple rt-pcr method that does not require rna extractio ... | 2015 | 25976592 |
| analysis of tospovirus nss proteins in suppression of systemic silencing. | rna silencing is a sequence-specific gene regulation mechanism that in plants also acts antiviral. in order to counteract antiviral rna silencing, viruses have evolved rna silencing suppressors (rss). in the case of tospoviruses, the non-structural nss protein has been identified as the rss. although the tomato spotted wilt virus (tswv) tospovirus nss protein has been shown to exhibit affinity to long and small dsrna molecules, its ability to suppress the non-cell autonomous part of rna silencin ... | 2015 | 26275304 |
| genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus tospovirus. | chrysanthemum stem necrosis virus (csnv) is a member of a tentative tospovirus species. in this study, the complete genomic sequence of the japanese csnv isolate tcch07a was determined. the l rna is 8960 nt long and encodes the 331.0-kda rna-dependent rna polymerase. the m rna is 4828 nt long and encodes the 34.1-kda movement protein (nsm) and the 127.7-kda glycoprotein precursor (gn/gc). the s rna is 2949 nt long and encodes the 52.4-kda silencing suppressor protein (nss) and the 29.3-kda nucle ... | 2015 | 25427981 |
| comparative analysis of chrysanthemum transcriptome in response to three rna viruses: cucumber mosaic virus, tomato spotted wilt virus and potato virus x. | the chrysanthemum is one of popular flowers in the world and a host for several viruses. so far, molecular interaction studies between the chrysanthemum and viruses are limited. in this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including cucumber mosaic virus (cmv), tomato spotted wilt virus (tswv) and potato virus x (pvx). a chrysanthemum 135k microarray derived from expressed sequence tags was successfully applied for the expression ... | 2015 | 25904110 |
| movement and nucleocapsid proteins coded by two tospovirus species interact through multiple binding regions in mixed infections. | negative-stranded tospoviruses (family: bunyaviridae) are among the most agronomically important viruses. some of the tospoviruses are known to exist as mixed infections in the same host plant. iris yellow spot virus (iysv) and tomato spotted wilt virus (tswv) were used to study virus-virus interaction in dually infected host plants. viral genes of both viruses were separately cloned into binary psite-bifc vectors. bifc results showed that the n and nsm proteins of iysv interact with their count ... | 2015 | 25666522 |
| thrips transmission of tospoviruses. | one hundred years ago, the disease tomato spotted wilt was first described in australia. since that time, knowledge of this disease caused by tomato spotted wilt virus (tswv) and transmitted by thrips (insects in the order thysanoptera) has revealed a complex relationship between the virus, vector, plant host, and environment. numerous tospoviruses and thrips vectors have been described, revealing diversity in plant host range and geographical distributions. advances in characterization of the t ... | 2015 | 26340723 |
| first complete genome sequence of a tomato spotted wilt virus isolate from the united states and its relationship to other tswv isolates of different geographic origin. | we report the first complete nucleotide sequence of a tomato spotted wilt virus (genus tospovirus, family bunyaviridae) isolate from the united states. the tripartite genome of pa01 consisted of l, m and s rnas of 8914, 4765 and 2984 nt, respectively. similarity percentages in nucleotide and amino acid sequence among pa01 and previously characterized tswv isolates are provided here. phylogenetic analysis on the rna-dependent rna polymerase (rdrp) gene placed pa01 in a different clade from an iso ... | 2015 | 26329831 |
| the silencing suppressor (nss) protein of the plant virus tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects. | in this work, we showed that cell death induced by a recombinant (vacnss) autographa californica multiple nucleopolyhedrovirus (acmnpv) expressing the silencing suppressor (nss) protein of tomato spotted wilt virus (tswv) was enhanced on permissive and semipermissive cell lines. the expression of a heterologous gene (firefly luciferase) during co-infection of insect cells with vacnss and a second recombinant baculovirus (vagppolhfluc) was shown to increase when compared to single vagppolhfluc in ... | 2015 | 26323262 |
| emergence of groundnut ringspot virus and tomato chlorotic spot virus in vegetables in florida and the southeastern united states. | groundnut ringspot virus (grsv) and tomato chlorotic spot virus (tcsv) are two emerging tospoviruses in florida. in a survey of the southeastern united states, grsv and tcsv were frequently detected in solanaceous crops and weeds with tospovirus-like symptoms in south florida, and occurred sympatrically with tomato spotted wilt virus (tswv) in tomato and pepper in south florida. tswv was the only tospovirus detected in other survey locations, with the exceptions of grsv from tomato (solanum lyco ... | 2015 | 25317844 |
| the movement proteins (nsm) of distinct tospoviruses peripherally associate with cellular membranes and interact with homologous and heterologous nsm and nucleocapsid proteins. | tospovirus is the only genus containing virus species which infect plants in the bunyaviridae family. the aims of this study were to understand the in vivo membrane association of the movement protein (nsm) of the tospovirus species bean necrotic mosaic virus, chrysanthemum stem necrosis virus, tomato chlorotic spot virus and tomato spotted wilt virus and the homologous and heterologous interactions among nsm and nucleocapsid protein (n). the results obtained by bimolecular fluorescence compleme ... | 2015 | 25705793 |
| stable reference gene selection for rt-qpcr analysis in nonviruliferous and viruliferous frankliniella occidentalis. | reverse transcriptase-quantitative polymerase chain reaction (rt-qpcr) is a reliable technique for measuring and evaluating gene expression during variable biological processes. to facilitate gene expression studies, normalization of genes of interest relative to stable reference genes is crucial. the western flower thrips frankliniella occidentalis (pergande) (thysanoptera: thripidae), the main vector of tomato spotted wilt virus (tswv), is a destructive invasive species. in this study, the exp ... | 2015 | 26244556 |
| small rna profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected nicotiana benthamiana. | tospoviruses are plant-infecting viruses belonging to the family bunyaviridae. we used a collection of wild-type, phylogenetically distinct tomato spotted wilt virus isolates and related silencing-suppressor defective mutants to study the effects on the small rna (srna) accumulation during infection of nicotiana benthamiana. our data showed that absence of a functional silencing suppressor determined a marked increase of the total amount of viral srnas (vsrnas), and specifically of the 21 nt cla ... | 2015 | 26047586 |
| phylogenetic analysis of tomato spotted wilt virus (tswv) nss protein demonstrates the isolated emergence of resistance-breaking strains in pepper. | resurgence of tomato spotted wilt virus (tswv) worldwide as well as in hungary causing heavy economic losses directed the attention to the factors contributing to the outbreak of this serious epidemics. the introgression of tsw resistance gene into various pepper cultivars seemed to solve tswv control, but widely used resistant pepper cultivars bearing the same, unique resistance locus evoked the rapid emergence of resistance-breaking (rb) tswv strains. in hungary, the sporadic appearance of rb ... | 2015 | 25331341 |
| the first complete genome sequences of two distinct european tomato spotted wilt virus isolates. | tomato spotted wilt virus (tswv) represents a major constraint to the production of important vegetable and ornamental crops in several countries around the world, including those in europe. in spite of their economic importance, european tswv isolates have only been partially characterized, and a complete genome sequence has not been determined yet. in this study, we completed the whole genome sequence of two distinct tswv isolates from italy, p105 and p202/3wt. the sequences of the l and m seg ... | 2015 | 25326756 |
| grafting on a non-transgenic tolerant tomato variety confers resistance to the infection of a sw5-breaking strain of tomato spotted wilt virus via rna silencing. | rna silencing controls endogenous gene expression and drives defensive reactions against invasive nucleic acids like viruses. in plants, it has been demonstrated that rna silencing can be transmitted through grafting between scions and silenced rootstocks to attenuate virus and viroid accumulation in the scions. this has been obtained mostly using transgenic plants, which may be a drawback in current agriculture. in the present study, we examined the dynamics of infection of a resistance-breakin ... | 2015 | 26496695 |
| diversity of thrips species and vectors of tomato spotted wilt virus in tomato production systems in kenya. | thrips have been recognized as primary vectors of tomato spotted wilt virus (tswv) with frankliniella occidentalis (pergande) reported as the most important and efficient vector, while other species such as thrips tabaci lindeman also include populations that can vector the virus. a study was undertaken to establish the diversity of thrips and presence of vectors for tswv in four major tomato production areas in kenya. the cytochrome oxidase 1 (co1) gene was used to generate sequences from thrip ... | 2015 | 26470099 |
| complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato sw-5 resistance-breaking and pepper tsw resistance-breaking) from spain. | tomato spotted wilt virus (tswv) occurs worldwide and causes production losses in many important horticultural crops such as tomato and pepper. breeding resistant cultivars has been the most successful method so far for tswv disease control, but only two genes have been found to confer resistance against a wide spectrum of tswv isolates: sw-5 in tomato and tsw in pepper. however, tswv resistance-breaking isolates have emerged in different countries a few years after using resistant cultivars. in ... | 2015 | 26026956 |
| untranslatable tospoviral nss fragment coupled with l conserved region enhances transgenic resistance against the homologous virus and a serologically unrelated tospovirus. | tospoviruses cause severe damages to important crops worldwide. in this study, nicotiana benthamiana transgenic lines carrying individual untranslatable constructs comprised of the conserved region of the l gene (denoted as l), the 5' half of nss coding sequence (nss) or the antisense fragment of whole n coding sequence (n) of watermelon silver mottle virus (wsmov), individually or in combination, were generated. a total of 15-17 transgenic n. benthamiana lines carrying individual transgenes wer ... | 2015 | 25721329 |
| development and utilization of indel markers to identify peanut (arachis hypogaea) disease resistance. | peanut diseases, such as leaf spot and spotted wilt caused by tomato spotted wilt virus, can significantly reduce yield and quality. application of marker assisted plant breeding requires the development and validation of different types of dna molecular markers. nearly 10,000 ssr-based molecular markers have been identified by various research groups around the world, but less than 14.5% showed polymorphism in peanut and only 6.4% have been mapped. low levels of polymorphism limit the applicati ... | 2015 | 26617627 |
| effects of thrips density, mode of inoculation, and plant age on tomato spotted wilt virus transmission in peanut plants. | spotted wilt caused by tomato spotted wilt virus (tswv; family bunyaviridae; genus tospovirus) is a serious disease of peanut (arachis hypogaea l.) in the southeastern united states. peanut genotypes with field resistance to tswv are effective in suppressing spotted wilt. all commercially available genotypes with field resistance to tswv were developed through conventional breeding. as a part of the breeding process, peanut genotypes are regularly screened under field situations. despite numerou ... | 2015 | 26308816 |
| structure and function analysis of nucleocapsid protein of tomato spotted wilt virus interacting with rna using homology modeling. | the nucleocapsid (n) protein of tomato spotted wilt virus (tswv) plays key roles in assembling genomic rna into ribonucleoprotein (rnp), which serves as a template for both viral gene transcription and genome replication. however, little is known about the molecular mechanism of how tswv n interacts with genomic rna. in this study, we demonstrated that tswv n protein forms a range of higher ordered oligomers. analysis of the rna binding behavior of n protein revealed that no specific oligomer bi ... | 2015 | 25540203 |
| evidence of a tomato spotted wilt virus resistance-breaking strain originated through natural reassortment between two evolutionary-distinct isolates. | a tsw resistance-breaking tomato spotted wilt virus field isolate (tswv-p331) found in northern italy originated via reassortment from two evolutionary distinct tswv strains, as revealed by recombination and phylogenetic analysis. compared to the closest isolate present in the database, p331 nss protein carries an unusually high number of amino acid substitutions, but no differences in the nucleocapsid protein. despite these substitutions, p331 nss is a potent silencing suppressor. as shown by p ... | 2015 | 25433286 |
| small rna profiles from virus-infected fresh market vegetables. | functional small rnas, such as short interfering rnas (sirnas) and micrornas (mirnas), exist in freshly consumed fruits and vegetables. these sirnas can be derived either from endogenous sequences or from viruses that infect them. symptomatic tomatoes, watermelons, zucchini, and onions were purchased from grocery stores and investigated by small rna sequencing. by aligning the obtained small rna sequences to sequences of known viruses, four different viruses were identified as infecting these fr ... | 2014 | 25389086 |
| host plant resistance against tomato spotted wilt virus in peanut (arachis hypogaea) and its impact on susceptibility to the virus, virus population genetics, and vector feeding behavior and survival. | tomato spotted wilt virus (tswv) severely affects peanut production in the southeastern united states. breeding efforts over the last three decades resulted in the release of numerous peanut genotypes with field resistance to tswv. the degree of field resistance in these genotypes has steadily increased over time, with recently released genotypes exhibiting a higher degree of field resistance than older genotypes. however, most new genotypes have never been evaluated in the greenhouse or laborat ... | 2014 | 24025049 |
| supervised learning and prediction of spatial epidemics. | parameter estimation for mechanistic models of infectious disease can be computationally intensive. nsoesie et al. (2011) introduced an approach for inference on infectious disease data based on the idea of supervised learning. their method involves simulating epidemics from various infectious disease models, and using classifiers built from the epidemic curve data to predict which model were most likely to have generated observed epidemic curves. they showed that the classification approach cou ... | 2014 | 25457597 |
| winter weeds as inoculum sources of tomato spotted wilt virus and as reservoirs for its vector, frankliniella fusca (thysanoptera: thripidae) in farmscapes of georgia. | thrips-transmitted tomato spotted wilt virus (tswv) has a broad host range including crops and weeds. in georgia, tswv is known to consistently affect peanut, tomato, pepper, and tobacco production. these crops are grown from march through november. in the crop-free period, weeds are presumed to serve as a green bridge for thrips and tswv. previous studies have identified several winter weeds as tswv and thrips hosts. however, their ability to influence tswv transmission in crops is still not co ... | 2014 | 24612539 |
| effect of watermelon silver mottle virus on the life history and feeding preference of thrips palmi. | thrips-borne tospoviruses cause numerous plant diseases that produce severe economic losses worldwide. in the disease system, thrips not only damage plants through feeding but also transmit causative agents of epidemics. in addition, thrips are infected with tospoviruses in the course of virus transmission. most studies on the effect of tospoviruses on vector thrips have focused on the tomato spotted wilt virus-frankliniella occidentalis system. thus, we focused on another thrips-borne tospoviru ... | 2014 | 25010157 |
| analysis of the a-u rich hairpin from the intergenic region of tospovirus s rna as target and inducer of rna silencing. | earlier work indicated that tomato spotted wilt virus (tswv) messenger transcripts, and not the (anti)genomic rnas, are targeted by the rna silencing machinery. here, the predicted au-rich hairpin (hp) structure encoded by the intergenic region (igr) of the tswv s rna, and present at the 3' end of viral mrnas, was analyzed as a target and inducer for rna silencing. virus-derived sirnas (vsirnas) purified from virus infected plants were found to derive from all three genomic rna segments but pred ... | 2014 | 25268120 |
| possible involvement of eef1a in tomato spotted wilt virus rna synthesis. | tomato spotted wilt virus (tswv) is a negative-strand rna virus in the family bunyaviridae and propagates in both insects and plants. although tswv can infect a wide range of plant species, host factors involved in viral rna synthesis of tswv in plants have not been characterized. in this report, we demonstrate that the cell-free extract derived from one of the host plants can activate mrna transcriptional activity of tswv. based on activity-guided fractionation of the cell-free extract, we iden ... | 2014 | 25151062 |
| disruption of vector transmission by a plant-expressed viral glycoprotein. | vector-borne viruses are a threat to human, animal, and plant health worldwide, requiring the development of novel strategies for their control. tomato spotted wilt virus (tswv) is one of the 10 most economically significant plant viruses and, together with other tospoviruses, is a threat to global food security. tswv is transmitted by thrips, including the western flower thrips, frankliniella occidentalis. previously, we demonstrated that the tswv glycoprotein gn binds to thrips vector midguts. ... | 2014 | 24405031 |
| control of viral diseases transmitted in a persistent manner by thrips in pepper (tomato spotted wilt virus). | tomato spotted wilt disease is caused by tomato spotted wilt virus (tswv) (tospovirus, bunyaviridae), a virus that severely damages and reduces the yield of many economically important plants worldwide and actually it is a major disease affecting the production of tomato and pepper in italy. due to the non-predictive nature of its outbreaks combined with the lack of forecasting, adoption of preventive measures have not always been practical, in fact the disease cycle has proven to be extremely d ... | 2014 | 26080477 |
| mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus tospovirus, family bunyaviridae). | tospoviruses cause serious economic losses to a wide range of field and horticultural crops on a global scale. the nss gene encoded by tospoviruses acts as a suppressor of host plant defense. we identified amino acid motifs that are conserved in all of the nss proteins of tospoviruses for which the sequence is known. using tomato spotted wilt virus (tswv) as a model, the role of these motifs in suppressor activity of nss was investigated. using site-directed point mutations in two conserved moti ... | 2014 | 24363189 |
| phylogenetic analysis of new zealand tomato spotted wilt virus isolates suggests likely incursion history scenarios and mechanisms for population evolution. | tomato spotted wilt virus (tswv) is an internationally significant pathogen with a wide host range, vectored by thrips. we have studied the sequence variation and evolutionary mechanisms at play in parts of the l, m and s subgenomes of 23 new zealand tswv isolates collected between 1992 and 2009, aiming to identify the possible geographic origins of isolates. maximum-likelihood-based phylogenetic analyses of new zealand and overseas tswv isolates placed the l and m subgenome sequences of two iso ... | 2014 | 24232914 |
| differential identification of three species of curtovirus using loop-mediated isothermal amplification. | rapid and sensitive detection methods for three species of curtovirus were developed using a loop-mediated isothermal amplification (lamp) technique. a universal primer set for detecting the three main species of curtovirus at the same time, and three kinds of species-specific primer sets were designed and used for lamp reactions. results from the lamp reactions were visualized both by color changes after adding sybr green i staining dye and by dna laddering on agarose gel electrophoresis. the o ... | 2014 | 24957721 |
| analysis of tomato spotted wilt virus nss protein indicates the importance of the n-terminal domain for avirulence and rna silencing suppression. | recently, tomato spotted wilt virus (tswv) nonstructural protein nss has been identified unambiguously as an avirulence (avr) determinant for tomato spotted wilt (tsw)-based resistance. the observation that nss from two natural resistance-breaking isolates had lost rna silencing suppressor (rss) activity and avr suggested a link between the two functions. to test this, a large set of nss mutants was generated by alanine substitutions in nss from resistance-inducing wild-type strains (nss(ri) ), ... | 2014 | 24103150 |
| broad-spectrum transgenic resistance against distinct tospovirus species at the genus level. | thrips-borne tospoviruses cause severe damage to crops worldwide. in this investigation, tobacco lines transgenic for individual wlm constructs containing the conserved motifs of the l rna-encoded rna-dependent rna polymerase (l) gene of watermelon silver mottle virus (wsmov) were generated by agrobacterium-mediated transformation. the wlm constructs included: (i) translatable wlm in a sense orientation; (ii) untranslatable wlmt with two stop codons; (iii) untranslatable wlmts with stop codons a ... | 2014 | 24811071 |
| the tomato spotted wilt virus cell-to-cell movement protein (nsm ) triggers a hypersensitive response in sw-5-containing resistant tomato lines and in nicotiana benthamiana transformed with the functional sw-5b resistance gene copy. | although the sw-5 gene cluster has been cloned, and sw-5b has been identified as the functional gene copy that confers resistance to tomato spotted wilt virus (tswv), its avirulence (avr) determinant has not been identified to date. nicotiana tabacum 'sr1' plants transformed with a copy of the sw-5b gene are immune without producing a clear visual response on challenge with tswv, whereas it is shown here that n. benthamiana transformed with sw-5b gives a rapid and conspicuous hypersensitive resp ... | 2014 | 24720811 |
| plant 4/1 protein: potential player in intracellular, cell-to-cell and long-distance signaling. | originally isolated as a result of its ability to interact with the movement protein of tomato spotted wilt virus in a yeast two-hybrid system, the 4/1 protein is proving to be an excellent tool for studying intracellular protein trafficking and intercellular communication. expression of 4/1 in vivo is tightly regulated, first appearing in the veins of the cotyledon and later in the vasculature of the leaf and stem in association with the xylem parenchyma and phloem parenchyma. structural studie ... | 2014 | 24611067 |
| coat protein mutations in an attenuated cucumber mosaic virus encoding mutant 2b protein that lacks rna silencing suppressor activity induces chlorosis with photosynthesis gene repression and chloroplast abnormalities in infected tobacco plants. | in tobacco plants, the cucumber mosaic virus (cmv) pepo strain induces mosaic symptoms, including pale green chlorosis and malformed tissues. here, we characterized the involvement of 2b protein and coat protein (cp) in the development of mosaic symptoms. a 2b mutant (r46c) that lacks viral suppressor of rna silencing (vsr) activity showed an asymptomatic phenotype with low levels of virus accumulation. tomato spotted wilt virus nss protein did not complement the virulence of the r46c, although ... | 2014 | 24889248 |
| the nss protein of tomato spotted wilt virus is required for persistent infection and transmission by frankliniella occidentalis. | tomato spotted wilt virus (tswv) is the type member of tospoviruses (genus tospovirus), plant-infecting viruses that cause severe damage to ornamental and vegetable crops. tospoviruses are transmitted by thrips in the circulative propagative mode. we generated a collection of nss-defective tswv isolates and showed that tswv coding for truncated nss protein could not be transmitted by frankliniella occidentalis. quantitative reverse transcription (rt)-pcr and immunostaining of individual insects ... | 2014 | 24623427 |
| the movement protein (nsm) of tomato spotted wilt virus is the avirulence determinant in the tomato sw-5 gene-based resistance. | the avirulence determinant triggering the resistance conferred by the tomato gene sw-5 against tomato spotted wilt virus (tswv) is still unresolved. sequence comparison showed two substitutions (c118y and t120n) in the movement protein nsm present only in tswv resistance-breaking (rb) isolates. in this work, transient expression of nsm of three tswv isolates [rb1 (t120n), rb2 (c118y) and non-resistance-breaking (nrb)] in nicotiana benthamiana expressing sw-5 showed a hypersensitive response (hr) ... | 2014 | 24690181 |
| non-structural proteins of arthropod-borne bunyaviruses: roles and functions. | viruses within the bunyaviridae family are tri-segmented, negative-stranded rna viruses. the family includes several emerging and re-emerging viruses of humans, animals and plants, such as rift valley fever virus, crimean-congo hemorrhagic fever virus, la crosse virus, schmallenberg virus and tomato spotted wilt virus. many bunyaviruses are arthropod-borne, so-called arboviruses. depending on the genus, bunyaviruses encode, in addition to the rna-dependent rna polymerase and the different struct ... | 2013 | 24100888 |
| oxylipin biosynthesis genes positively regulate programmed cell death during compatible infections with the synergistic pair potato virus x-potato virus y and tomato spotted wilt virus. | one of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis ... | 2013 | 23487466 |
| tsw gene-based resistance is triggered by a functional rna silencing suppressor protein of the tomato spotted wilt virus. | as a result of contradictory reports, the avirulence (avr) determinant that triggers tsw gene-based resistance in capsicum annuum against the tomato spotted wilt virus (tswv) is still unresolved. here, the n and nss genes of resistance-inducing (ri) and resistance-breaking (rb) isolates were cloned and transiently expressed in resistant capsicum plants to determine the identity of the avr protein. it was shown that the nss(ri) protein triggered a hypersensitive response (hr) in tsw-containing ca ... | 2013 | 23360130 |
| resistance breaking tomato spotted wilt virus isolates on resistant pepper varieties in italy. | in spring 2012, resistance breaking (rb) isolates of tomato spotted wilt virus (tswv) that overcome the resistance conferred by the tsw gene in different pepper hybrids have been recovered in different locations in southern italy (campania and apulia regions) in protected cultivation, about one month after transplant. the percentage of symptomatic plants was 5-10% and, only in particular cases of advanced stage of cultivation, it reached 30-50% at the end of cycle. all tswv isolates induced simi ... | 2013 | 25151838 |
| development of apple latent spherical virus-based vaccines against three tospoviruses. | apple latent spherical virus (alsv) is characterized by its relatively broad host range, latency in most host plants, and ability to induce gene silencing in host plants. herein, we focus on the above characteristic of alsv and describe our development of alsv vector vaccines against three tospoviruses, namely, impatiens necrotic spot virus (insv), iris yellow spot virus (iysv), and tomato spotted wilt virus (tswv). dna fragments of 201 nt of three tospovirus s-rnas (silencing suppressor (nss) a ... | 2013 | 23850843 |
| development of a real-time fluorescent quantitative pcr assay for detection of impatiens necrotic spot virus. | impatiens necrotic spot virus (insv) is an important plant virus that can cause severe disease in various ornamental and agricultural crops. several species of thrips transmit insv, of which the western flower thrip (frankliniella occidentalis) is the most important. in this study, primers and taqman probes based on insv non-structural protein gene sequences were designed, and a technique was developed for detecting insv using fluorescent quantitative rt-pcr. the reproducibility, specificity and ... | 2013 | 23466630 |
| differential expression of tomato spotted wilt virus-derived viral small rnas in infected commercial and experimental host plants. | viral small rnas (vsirnas) in the infected host can be generated from viral double-stranded rna replicative intermediates, self-complementary regions of the viral genome or from the action of host rna-dependent rna polymerases on viral templates. the vsirna abundance and profile as well as the endogenous small rna population can vary between different hosts infected by the same virus influencing viral pathogenicity and host response. there are no reports on the analysis of vsirnas of tomato spot ... | 2013 | 24143182 |
| interacting virus abundance and transmission intensity underlie tomato spotted wilt virus incidence: an example weather-based model for cultivated tobacco. | through a modeling approach, we investigated weather factors that affect the summer incidence of tomato spotted wilt virus (tswv), a virus vectored exclusively by thrips, in cultivated tobacco. aspects of thrips and plant biology that affect disease spread were treated as functions of weather, leading to a model of disease incidence informed by thrips and plant biology, and dependent on weather input variables. we found that disease incidence during the summer was influenced by weather affecting ... | 2013 | 23977384 |
| tomato spotted wilt virus infection reduces the fitness of a nonvector herbivore on pepper. | plant pathogens and insect herbivores often share hosts under natural conditions. hence, pathogen-induced changes in a host plant can affect the herbivore and vice versa. even though plant viruses are ubiquitous in the field, little is known about plant-mediated interactions between viruses and nonvector herbivores. here we tested whether the performance of the sweet potato whitefly, bemisia tabaci (gennadius) biotype q, was altered when raised on pepper infected with tomato spotted wilt virus ( ... | 2013 | 23786083 |
| thrips tabaci population genetic structure and polyploidy in relation to competency as a vector of tomato spotted wilt virus. | knowledge of population-level genetic differences can help explain variation among populations of insect vectors in their role in the epidemiology of specific viruses. variation in competency to transmit tomato spotted wilt virus (tswv) that exists among populations of thrips tabaci has been associated with the presence of cryptic species that exhibit different modes of reproduction and host ranges. however, recent findings suggest that vector competency of t. tabaci at any given location depend ... | 2013 | 23365671 |
| specific insect-virus interactions are responsible for variation in competency of different thrips tabaci isolines to transmit different tomato spotted wilt virus isolates. | local adaptation between sympatric host and parasite populations driven by vector genetics appears to be a factor that influences dynamics of disease epidemics and evolution of insect-vectored viruses. although t. tabaci is the primary vector of tomato spotted wilt virus (tswv) in some areas of the world, it is not an important vector of this economically important plant virus in many areas where it occurs. previous studies suggest that genetic variation of thrips populations, virus isolates, or ... | 2013 | 23358707 |
| second generation peanut genotypes resistant to thrips-transmitted tomato spotted wilt virus exhibit tolerance rather than true resistance and differentially affect thrips fitness. | spotted wilt disease caused by tomato spotted wilt virus (tswv) (family bunyaviridae; genus tospovirus) is a major constraint to peanut (arachis hypogaea l.) production in the southeastern united states. reducing yield losses to tswv has heavily relied on planting genotypes that reduce the incidence of spotted wilt disease. however, mechanisms conferring resistance to tswv have not been identified in these genotypes. furthermore, no information is available on how these genotypes influence thrip ... | 2013 | 23786043 |
| from immunity to susceptibility: virus resistance induced in tomato by a silenced transgene is lost as tgs overcomes ptgs. | tomato line 30.4 was obtained engineering the nucleocapsid (n) gene of tomato spotted wilt virus into plant genome, and immunity to tomato spotted wilt virus infection of its self-pollinated homozygous progeny was observed. despite the presence of a high amount of transgenic transcripts, transgenic proteins have not been detected, suggesting a mechanism of resistance mediated by rna. in the present study, we identify post-transcriptional gene silencing as the main mechanism of resistance, which ... | 2013 | 23738576 |