[study on natural infection of epidemic haemorrhagic fever virus in leptotrombidium scutellare].leptotrombidium (l.) scutellare was considered to be the suspected vector of epidemic haemorrhagic fever (ehf). in order to clarify the relationship between this mite and ehf, from oct to nov 1988 and oct of 1989, the larvae of this mite were collected from rats in areas with constantly high incidence of ehf in shanxi province and larvae with only small quantity of meal were selected and for more than 15 days before isolation of ehfv. six strains of virus were isolated. the viruses isolated were ...19902124535
molecular characterization of the rna s segment of nephropathia epidemica virus strain hällnäs b1.the s segment rna of nephropathia epidemica virus (nev) strain hällnäs b1 was isolated by molecular cloning of the corresponding cdna. the rna is 1785 nucleotides long with the 3' and 5' termini being complementary for 23 bases. the viral messenger-sense rna contains one major open reading frame (orf) with a coding capacity of 433 amino acids encoding a 49-kda polypeptide. compared to the hantaan s segment cdna sequence there is a nucleotide homology of 60 and 61% at the amino acid level. many o ...19902104684
[virus-specific proteins and rna of the virus of hemorrhagic fever with renal syndrome].virus-specific proteins g1, g2, and n with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in vero e-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (hfrs) isolated in the european ussr from a patient with hfrs, a fatal human case of hfrs (the strains k-27 and p-360) and from a bank vole (strain cg-1820). the sera from human convalescents after hfrs in the european ussr and rat sera prepared with the cg ...19902114054
nucleotide sequence of the m genome segment of hemorrhagic fever with renal syndrome virus strain b-1. 19902118626
coding strategy of the s rna segment of dugbe virus (nairovirus; bunyaviridae).the s rna segment of dugbe (dug) virus (nairovirus; bunyaviridae) was sequenced from three overlapping cdna clones and by primer extension. the s rna is 1712 nucleotides in length and contains one large open reading frame (orf) of 1326 nucleotides coding for a 49.4-kda protein on viral complementary (vc) rna. this protein in size corresponds to the dug nucleocapsid (n) protein (p. cash, 1985, j. gen. virol. 66, 141-148). the 49.4-kda product was expressed as a fusion protein with beta-galactosid ...19902327076
distinction between bunyaviridae genera by surface structure and comparison with hantaan virus using negative stain electron microscopy.ultrastructural studies of glutaraldehyde-fixed viruses of the bunyaviridae were performed by negative-stain electron microscopy. the surface structure of viruses of each genus was compared with that of the other genera and with hantaan virus, the prototype of a proposed new genus of bunyaviridae. viruses of each genus had a surface structure distinct for that genus. in addition, hantaan virus had a surface structure composed of a grid-like pattern of morphologic subunits not previously describe ...19852412528
[circulation of the virus of hemorrhagic fever with renal syndrome among small mammals in the territory of the ussr].over 55,000 small mammals of 72 species trapped in 62 administrative territories practically in all landscape-geographical zones of the ussr were examined in 1980-1985. the use of current laboratory methods demonstrated a wide spread of hfrs virus in the territory of this country, involvement in the epizootic process of most species of forest and steppe murine rodents and insectivora. in each landscape zone, the main carriers of the hfrs agent were established, represented, as a rule, by the bas ...19872451354
[antigenic components of the epidemic hemorrhagic fever virus]. 19872443227
effects of human and murine interferons against hemorrhagic fever with renal syndrome (hfrs) virus (hantaan virus).the effects of human interferons (ifn-alpha, ifn-beta, ifn-gamma) on the replication of hantaan virus (hv) in vero e6 cells were examined. pretreatment of cells with human ifns resulted in dose-dependent inhibition of hv plaque formation. of the 3 human ifns, ifn-beta inhibited virus replication most effectively. pretreatment of murine macrophage cells with mouse ifn-beta also resulted in an inhibition of viral growth and then the effect of murine ifn-beta in newborn icr mice infected with hv wa ...19872451468
characterization of hantaan virus envelope glycoprotein antigenic determinants defined by monoclonal antibodies.a panel of 24 monoclonal antibodies (mabs) to the g1 or g2 envelope glycoproteins of hantaan virus were used to determine the surface topography and functional properties of antigenic sites. nine distinct, partially overlapping antigenic sites, two on g1 and seven on g2, were demonstrated by competitive binding assays. analyses of the antigenic sites by haemagglutination (ha) inhibition and plaque-reduction neutralization tests showed that all of the sites, except one on g1, were related to vira ...19892471792
new isolates of hfrs virus in sichuan, china and characterisation of antigenic differences by monoclonal antibodies. 19862423827
virion proteins of viruses causing hemorrhagic fever with renal syndrome; monoclonal antibody analysis.sds-polyacrylamide gel electrophoresis and western blotting analysis by using 9 clones of monoclonal antibodies specific for hemorrhagic fever with renal syndrome (hfrs) viruses were carried out on the virion proteins of 17 strains of hfrs viruses isolated from different areas in asia and different hosts such as apodemus agrarius, rattus norvegicus, domestic cat and hfrs patients. polypeptide with apparent molecular weight about 50 kitodalton (kd) could be detected in all 17 strains of hfrs viru ...19892474668
incidence of antibodies against human immunodeficiency virus, human t-cell lymphotropic virus type 1, hepatitis b virus, hemorrhagic fever with renal syndrome virus and chlamydia in tonga and western samoa.among the populations of tonga and western samoa, serum antibodies against human immunodeficiency virus or hemorrhagic fever with renal syndrome virus were not detected (0/904 and 0/192). no serum samples were considered to be positive for antibody against human t-cell lymphotropic virus type 1 (0/527). hepatitis b antigen and antibody were found in 4% (8/192) and 47% (90/192), respectively. chlamydia trachomatis igg and c. psittaci igg antibodies were detected in 39% (75/192) and 47% (91/192), ...19902126048
[preliminary study on the virulent difference between viral strains isolated from rattus norvegicus and apodemus agrarius in fujian province].virulence between virus strains of hemorrhagic fever with renal syndrome (hfrs) from r. norvegicus and a. agrarius were different, besides the diversity in their epidemiological feature, clinical symptoms and antigenicity. virulence of the virus strains from r. norvegicus, especially those from nonepidemic areas, were weak than those from a. agrarius. sucking mice might be used for determining virulence of the virus strains from various source. foci of hfrs had been in southern fujian, but human ...19892567628
prevalence of antibody to hantavirus in some cat populations in britain. 19902177930
[antigenicity analysis of hemorrhagic fever with renal syndrome viruses by mcabs i]. 19872450686
[preliminary study on epidemic hemorrhagic fever (ehf) in guizhou province].during 1962-1986, ehf cases have been reported in 50 out of 82 counties in guizhou province. it was high in northern guizhou province. however, serological survey of the population showed that endemic area extended to 51 counties. the inapparent infection rate ranged from 0 to 4.04%. in 76 out of 82 counties the rodents were found to carry hantavirus. apodemus agrarius of zunyi county of guizhou and rattus norvegicus of the eastern and south guizhou are important reservoirs of ehf. hantavirus ha ...19892567626
[isolation of strains of the virus of hemorrhagic fever with renal syndrome in a cell culture from human patients and rodents].the virus of hemorrhagic fever with renal syndrome serologically similar to hantaan virus was isolated from three rodent species, ap. peninsulae, cl. rutilus, cl. rufocanus, and from human patients in vero e6 cell culture.19862425494
[studies on inapparent infection of epidemic hemorrhagic fever in population of shanxi province].detection for igg antibody to virus of epidemic hemorrhagic fever (ehf) in sera from healthy people in shanxi province were carried out by ifat. two hundred thirty eight of 4873 serum samples were antibody-positive. total positive rate was 4.88%. the inapparent infection rate of ehf in residents of endemic areas was 5.48%. there was a statistically significant difference of epidemic strength among different counties. based on the analysis of antibody titers of positive sera, value of upper limit ...19892567627
[study on geographic epidemiology of epidemic hemorrhagic fever (ehf) in china].the distribution, epidemic intensity and the focus type of ehf at county's level in china was studied by the method of discriptive, serological and geographic epidemiology. the ehf foci distributed in 1,257 counties within 25 provinces (cities, autonomous regions) by 1986, 633 of them were found before 1980 and the other 624 after it. the epidemic area have extended nearly twice in the last years. the extension of rattus type focus was most significant. the number of focus of rattus type increas ...19892567630
[classification of hfrs viruses]. 19892566698
experimental labyrinthitis in rats caused by infecting with virus causing hemorrhagic fever with renal syndrome (hfrs).acute and persistent viral infection of the inner ear of rats was induced with hemorrhagic fever with renal syndrome (hfrs) virus following intraperitoneal inoculation. newborn (within 24 h after birth) rats were inoculated intraperitoneally (i.p.) with hfrs virus (b-1 strain), and the cochlea was examined using an immunohistochemical technique for the localization of viral antigens 1 week (acute infection) and 1 year (persistent infection) after infection. virus-specific antigens were detected ...19892504018
comparison of virulence between two strains of rattus serotype hemorrhagic fever with renal syndrome (hfrs) virus in newborn rats.two strains of hemorrhagic fever with renal syndrome (hfrs) virus from rattus, sr-11 and ki-262, showed virtually identical antigenicity but differed from prototype strain hantaan 76-118 (apodemus origin) in a neutralization test. wistar newborn rats inoculated intraperitoneally (i.p.) with sr-11, which was isolated from a laboratory rat associated with an outbreak of hfrs, developed clinical signs such as ataxia and limb paralysis and died at about 18 days after inoculation. the ld50 of sr-11 i ...19892566896
[area and natural reservoirs of hemorrhagic fever virus with kidney syndrome in the far east of the ussr].lungs of 9127 small mammals of 17 species trapped in khabarovsk region, magadan, amur, and sakhalin regions in 1982-1987 were examined, among them 11 species are reservoirs of hfrs virus. most frequently infected are striped field mice and japanese field mice, red voles and large-toothed red-backed voles which are the dominant species of the appropriate landscape formations. circulation of two hfrs virus serotypes among small mammals was demonstrated. the main epidemiological role belongs to the ...19892514490
[hemorrhagic fever with renal syndrome]. 19892568275
rapid detection of genomic variations in different strains of hantaviruses by polymerase chain reaction techniques and nucleotide sequence analysis.the polymerase chain reaction (pcr) with subsequent nucleotide sequence analysis was employed to rapidly detect genomic variations among different hantavirus strains. using synthetic oligonucleotide primers derived from the m and s segment rnas of nephropathia epidemica virus strain hällnäs b1 (nev) we succeeded in amplifying the corresponding sequences of hantaan and puumala viruses. the nucleotide sequences of the cdnas derived from the puumala m and s rna segments were analyzed. it was found ...19902385957
diagnostic complementary dna probes for genome segments 2 and 3 of epizootic hemorrhagic disease virus serotype 1.potential diagnostic complementary dna (cdna) clones of gene segments 2 and 3 from epizootic hemorrhagic disease virus serotype 1 (ehdv-1) have been produced. individual segments of ehdv-1 were isolated, denatured with methylmercury hydroxide, and polyadenylated. the polyadenylated rna was reverse-transcribed and self-hybridized into duplex structures, and the incomplete ends were repaired. the resulting product was then cloned into the plasmid vector pbr322, using the complementary tailing meth ...19902164331
[clinical and epidemiologic characteristics of hemorrhagic fever with renal syndrome].clinical and epidemiological data on 16 patients with hfrs admitted to the university hospital of infectious diseases in zagreb during the past 10 years (1977-1986) are reported. in 13 of them, the diagnosis was confirmed serologically by indirect fluorescent method. all but two were men between 20 and 45 years of age (80% of patients). the disease appeared sporadically only. according to the domicile, way of living and working all the patients but three mentioned the contact with rodents in the ...19892568568
serological studies of haemorrhagic fever with renal syndrome (hfrs) in poland. preliminary report.serological studies of hantaan virus infections among--blood donors (control group)--patients: chronic dialysed, leptospirosis suspected, hospitalised for various diseases,--risk group population: farmers, laboratory staff (contact with laboratory animals), as well as laboratory and wild animals (rodents) were carried out. surveys were done by the indirect immunofluorescent antibody (ifa) procedure with 5 hantaan virus strains as antigens.19892476002
[hemorrhagic fever with renal syndrome (hfrs) in japan]. 19892566697
immunoblot analysis of the serological response in hantavirus infections.sera from patients with nephropathia epidemica (ne) or korean hemorrhagic fever (khf) were tested for specific antibody response to antigens of hällnäs virus and hantaan virus strain 76-118. a vero e6 derived cell line persistently infected with hällnäs virus strain b1, and vero e6 cells freshly infected with hantaan virus type strain 76-118 were used as antigens in the immunofluorescence assay (ifa) and the immunoblot. blots were prepared from whole cell lysates. the convalescent-phase sera of ...19892566644
[study on human monoclonal antibodies in hemorrhagic fever with renal syndrome virus]. 19892550116
comparison of immunofluorescence and enzyme-linked immunosorbent assays for the serology of hantaan virus infections.three enzyme-linked immunosorbent assay (elisa) systems based upon different principles were developed for the serology of hantaan virus infections and compared with an indirect immunofluorescence assay (ifa). the indirect ifa was carried out with gamma-irradiated hantaan virus-infected and uninfected vero e6 cells fixed with ethanol (-70 degrees c) or acetone (20 degrees c) on drop slides and a fitc-coupled sheep anti-human ig preparation. atypical staining in the ifa was avoided by using ethan ...19892566619
characterization of glycoproteins of viruses causing hemorrhagic fever with renal syndrome (hfrs) using monoclonal antibodies.viruses causing hemorrhagic fever with renal syndrome (hfrs) encode two glycoproteins, g1 and g2. for determination of the biological functions of these glycoproteins, we isolated 15 hybridomas secreting monoclonal antibodies directed against the glycoproteins of the b-1 and hantaan viruses (hv). from results of neutralizing and hemagglutination inhibition (hi) tests, and studies on the antigenic reactivities of the antibodies with other hv-related viruses by immunofluorescence, we classified th ...19862422813
[reservoirs of the causative agent of hemorrhagic fever with renal syndrome in maritime territory foci].examinations of 6362 small mammals of 18 species revealed the main reservoirs of the causative agent of hemorrhagic fever with the renal syndrome (hfrs) in foci of the primorsky krai, and demonstrated the importance of individual species in the epidemiology of the infection. data on the circulation in the region of antigenically different serotypes of hfrs virus are presented.19852408384
cross-reactive immunity among different serotypes of virus causing haemorrhagic fever with renal syndrome.spleen cells primed by prospect hill (ph) or puumala (pu) virus could cross-react with hantaan virus (hv) 76-118 strain-infected target cells after in vitro stimulation with hv-infected cells, although anti-ph or anti-pu immune serum showed no cross-reactive neutralizing (nt) activity to hv without complement. these results and our previous findings with cross-reactive cytotoxic t lymphocytes (ctls) suggest that some epitopes recognized by ctls might be common among the hantavirus genus, while t ...19892567343
[extraction of effective parts of alternanthera philoxeroides (mart.) griseb. and its antiviral effect].several parts of alternanthera philoxeroides were extracted by lead-salt sedimentation and double phase solvent extractive technique and their antiviral effects were studied. the results showed that extracts of petroleum ether, ether, and ethyl acetate have inhibitory effect on epidemic hemorrhagic fever virus (ehfv). the antiviral effective part is coumarin analogue.19892508673
plasma concentration of atrial natriuretic peptide in different phases of korean hemorrhagic fever.korean hemorrhagic fever (khf) is an epidemic viral disease characterized by high fever, hemorrhagic tendency and renal failure, and by hemorrhages of right atrium and renal medulla as well as necrosis of anterior hypophysis. plasma immunoreactive atrial natriuretic peptide (iranp) levels of 15 patients in the oliguric phase was 94.8 +/- 8.4 pg/ml (mean +/- sem), 80% higher than of the normal control group (53.0 +/- 4.7 pg/ml; n = 28). in the diuretic phase it declined to 63.7 +/- 5.3 pg/ml (n = ...19892563575
characterization of hantaviruses using monoclonal antibodies.the antigenic relations among nine hantaviruses from seven different geographic areas were examined in indirect fluorescent antibody test with 12 monoclonal antibodies. ten monoclonals were prepared against hantaan virus strain 76-118 and two against hantavirus b-1, a virus isolated from a tumour in a laboratory rat. four different serotypes have been differentiated: hällnäs, hantaan, prospect hill and rat strain like. two monoclonal antibodies were able to react with all nine hantaviruses studi ...19872449811
determination of the coding capacity of the m genome segment of nephropathia epidemica virus strain hällnäs b1 by molecular cloning and nucleotide sequence analysis.the m genome rna segment of nephropathia epidemica virus (nev) strain hällnäs b1 was characterized by molecular cloning and dna nucleotide sequencing of the corresponding cdna clones. the size of the m rna segment is 3682 nucleotides. the 3' and 5' terminal sequences are complementary for 21 bases and their predicted secondary structure is very stable. the viral complementary messenger rna possesses a single long open reading frame with a coding capacity of 1148 amino acids (polypeptide of 126 k ...19892508317
hemorrhagic fever with renal syndrome: four decades of research.hemorrhagic fever with renal syndrome is an acute infectious illness characterized by fever, hemorrhage, and renal failure. research over the last 40 years has led to the discovery and characterization of the causative viruses, detailed knowledge of the epidemiology of the disease, development of sensitive diagnostic assays, and improvements in patient management, which in turn have led to significant reductions in mortality. considerable progress has also been made in elucidating the pathophysi ...19892563323
antibody prevalence against haemorrhagic fever viruses in randomized representative central african populations.between 1985 and 1987, 5,070 randomly selected persons living in 6 central african countries (cameroon, central african republic, chad, congo, equatorial guinea and gabon) were checked for serological evidence of haemorrhagic fever. rural and urban areas were studied, including ecoclimatic zones ranging from dry savana to tropical rain forest. virus-reactive antibodies were found with all antigens tested, and the global prevalence of positive sera was distributed as follows: crimean-congo haemor ...19892505350
[antibodies against hantaan virus and leptospira in subjects at risk in rome].a survey on the prevalence of hantaan and leptospiral antibodies on mammalogists and rodent control personnel was performed. none of the 66 trappers studied (using ifi ) had detectable hantaan antibody, while only 2 out of 20 mammalogists presented antibody at low titer (1:32). for leptospiral antibody the microagglutination test (mat) using live leptospires as antigen was performed. 14 out of 66 trappers, or 21.2 per cent, had antibodies, at titer of 1:50 or more, to various leptospiral serovar ...19892518821
serologic evidence of hantaviral infections within small mammal communities of baltimore, maryland: spatial and temporal patterns and host range.serologic evidence was used to investigate the spatial and temporal distribution and host range of hantaviruses in small mammal communities in baltimore, md. immunofluorescent antibody (ifa) reactive to a baltimore rat isolate of seoul virus was detected in 44% of 404 norway rats captured at 4 park or residential locations; 21% of 121 meadow voles captured at 4 park locations possessed significant ifa titers to prospect hill virus. evidence from plaque neutralization assay of rodent sera suggest ...19892570533
tonic pupillary reaction after epidemic nephropathy and transient myopia. 19892569273
characteristics of passive immunity against hantavirus infection in rats.the protective effects of passively administered antibodies against hantavirus infection were studied in newborn rats. death as well as infection were completely prevented from intraperitoneal challenge of strain sr-11 (sr) (2 x 10(3) ffu, 10(2.1) ld50), in newborn rats which received 0.1 ml of anti-sr rat serum (neutralizing antibody titer, 1:640) 4 hr before the virus challenge. in these rats, no virus was detected in the peritoneal macrophages, lung, kidney, and brain. the immune serum infusi ...19892568827
hantavirus nephropathy in netherlands. 19892569143
a sporadic case of acute hantavirus nephropathy in the netherlands.a 32-yr-old man presented with acute renal failure preceded by a viral-like disease with high fever, bilateral loin pain, nausea, headache and slight thrombocytopenia. renal biopsy revealed only minor tubulointerstitial abnormalities. renal function completely normalised within 10 days after hospitalisation. the diagnosis of acute hantavirus nephropathy was proved by serological examinations.19892571092
the duration of viremia patients with epidemic hemorrhagic fever.the duration of viremia in patients with epidemic hemorrhagic fever (ehf) was studied using immunofluorescence technique and cell culture assays. the duration of virus in plasma of ehf patients was about 1 week, which was concomitant with the febrile phase of the disease. comparatively, the separation rate of viruses in peripheral blood mononuclear cells (pbmc) was greater (1.8 times) than in plasma, while the detected peak of ehf (in 4-7 days after onset of the disease) was found to be 2 or 3 d ...19892570679
[the immune structure of the population of the bashkir assr in connection with hemorrhagic fever with renal syndrome and the relation of indices of collective immunity to morbidity and the nature of the landscape].the study of 9,176 serum samples obtained from all regions and 15 cities of bashkiria, carried out by the method of radioimmunoassay with the use of the antigen of hemorrhagic fever with the renal syndrome (hfrs), has revealed considerable differences in the size of the natural immune stratum of the population (43.9-0.8%). the size of such immune stratum characterizes the activity of natural foci: the largest immune stratum (25.4%) exists among the population of regions with broad-leaved forests ...19892573985
[features of a natural focus of hemorrhagic fever with a kidney syndrome in various regions of the crimea]. 19892573971
clinical characteristics of nephropathia epidemica in sweden: prospective study of 74 cases.clinical symptoms and laboratory measures of renal and liver function, coagulation, and inflammatory parameters were prospectively studied in 74 hospitalized patients (14-74 years of age) with serologic evidence of nephropathia epidemica. the most common clinical findings were acute onset of symptoms, fever (greater than or equal to 38 degrees c), thirst, headache, nausea, back pain, vomiting, myalgia, and abdominal pain. twenty-seven patients (37%) had hemorrhagic manifestations, i.e., epistaxi ...19892574903
hemorrhagic fever with renal syndrome in yugoslavia: antigenic characterization of hantaviruses isolated from apodemus flavicollis and clethrionomys glareolus.hantavirus antigens were detected in lung tissues of 8/113 apodemus flavicollis and 2/17 clethrionomys glareolus captured in 1984 in fojnica, a region of yugoslavia endemic for hemorrhagic fever with renal syndrome; hantavirus antigens were not detected in lung tissues from 126 other mammals collected in fojnica. three hantaviruses, 2 from a. flavicollis and 1 from c. glareolus, were isolated directly in vero e6 cells and were partially characterized. the isolates from a. flavicollis, designated ...19892569846
the distribution and duration of hantaan virus in the body fluids of patients with hemorrhagic fever with renal syndrome.the distribution and duration of hantaan virus (htnv) in the body fluids of patients were studied by immunofluorescence, reverse passive hemagglutination, and cell culture assays. virus antigen of hemorrhagic fever with renal syndrome in peripheral blood mononuclear cells (pbmcs) was usually present before day 11 of the disease, especially from days 4-7. virus isolates were more readily recovered from plasma early in the course of the illness and less frequently after day 7. the use of pbmcs rat ...19892569493
renal involvement in hantavirus disease. 19892571242
epidemic hemorrhagic fever with renal syndrome in yugoslavia, epidemic of hemorrhagic fever with renal syndrome (hfrs) occurred in yugoslavia may-november 1986; all republics and provinces were involved. serum samples were received from 260 of 276 persons with symptoms clinically compatible with a diagnosis of hfrs. presumptive infection with a hantavirus was determined serologically for 161 of these. many patients with serious clinical pictures, including severe renal insufficiency and shock, were hospitalized; 11 died. indirect fluorescent antibody te ...19892569845
evidence for hantavirus disease in slovenia, slovenia, north-western part of yugoslavia, 17 clinically documented hantavirus disease cases (hvd) were serologically confirmed so far. previously hvd was reported in the southern part of yugoslavia. by the indirect fluorescent antibody test (ifa), the prevalence of igg class antibodies against different hantaviral antigens was demonstrated in human sera collected in slovenia. three different reactivity patterns were observed. majority of the ifa-positive human sera were confirmed by the imm ...19892574939
[the incidence and clinical picture of hemorrhagic fever with renal syndrome in east slovakia: the eastern and western type].hemorrhagic fever with renal syndrome (hfrs) is endemic in east slovakia. the paper presents the clinical picture of 4 serologically confirmed cases. two of the 4 patients had serological evidence of recent hantaan virus infection. positive immunofluorescent reactions with puumala virus (cg 18-20) were detected in the sera of the other two patients. the obtained results suggest the circulation of two serotypes of hfrs in slovakia.19892574625
[epidemic hemorrhagic fever during pregnancy]. 19892575026
hemorrhagic fever with renal syndrome in korea.several clinical variants of hemorrhagic fever with renal syndrome (hfrs) are caused by hantaan and related viruses. since 1951, 500-900 patients with hfrs have been hospitalized annually in korea. although hfrs is associated primarily with rural areas, it is now being recognized as an urban problem and a particular hazard to laboratory staff using rodents for research. recently, epidemic outbreaks of leptospirosis and scrub typhus have occurred during the hfrs season, leading to confusion in di ...19892568676
[another case of hantaan virus infection in the netherlands].in a serological survey among dutch patients suspected of leptospirosis, using a recently developed enzyme-linked immunosorbent assay, a patient was traced with a high antibody titre to hantaan virus. no anti-leptospira antibodies were detected in this 27-year-old man. shortly before he had been admitted to the hospital with progressive dyspnoea and coughing, accompanied with high fever. an interstitial pneumonia was diagnosed. he subsequently developed a progressive renal failure with proteinur ...19892572978
what is new and where are the problems in research on hemorrhagic fever with renal syndrome? 19892573193
the prevalence of hantaan viruses among rattus norvegicus in tokyo in the 1960's.during the 1960's, 15 out of 89 rats (16.8%) captured in the port area and 6 out of 59 rats (10.2%) in the suburb of tokyo were seropositive against hantaan viruses.19892573224
pathogenesis of hantaan virus in mice.the virulence of two virus clones (hv cl-1 and hv cl-2) of hantaan virus, which were plaque-purified on vero e6 cells, were compared in suckling mice infected by the intraperitoneal or intracerebral route. hv cl-1 increased the mortality of the mice whereas hv cl-2 did not. furthermore, virus of high titre was isolated from various organs of mice infected with hv cl-1 and high titres were maintained, whereas after infection with hv cl-2, virus was isolated from various organs only in low titre a ...19892573685
hemorrhagic fever with renal syndrome.hantaviruses, the causative agents of hfrs, have become more widely recognized. epidemiologic evidence indicates that these pathogens are distributed worldwide. people who come into close contact with infected rodents in urban, rural and laboratory environments are at particular risk. transmission to man occurs mainly via the respiratory tract. the epidemiology of the hantaviruses is intimately linked to the ecology of their principal vertebrate hosts. four distinct viruses are now recognized wi ...19892573914
[infection of human endothelial cells by the virus causing hemorrhagic fever with renal syndrome]. 19892575927
[tubulointerstitial changes in patients with a history of hemorrhagic fever with renal syndrome].altogether 85 patients with a history of hemorrhagic fever and the nephrotic syndrome (hfns) were examined at different stages after the disease, characterizing the status of the tubulointerstitial apparatus of the kidneys. almost half of the patients who suffered this disease manifested a decrease in the capacity of the kidneys for osmotic concentration of urine under the conditions of deprivation for 36 hours, and in acid-secretory function of the kidneys under induced acidosis and clearance o ...19892576473
effects of hantaviral infection on survival, growth and fertility in wild rat (rattus norvegicus) populations of baltimore, maryland.survival, growth rates, body size and fertility of wild caught norway rats (rattus norvegicus), infected and uninfected with a hantavirus (antigenically related to seoul virus), were compared. no differences were found in the survival of seronegative versus seropositive rats, as measured by mark-recapture experiments. growth rates, as measured by weight gain but not by increased body length, were slower in seropositive, sexually mature (greater than 200 g) rats, although no differences in the ul ...19892572705
serological survey of hantavirus infection in french polynesia. 19892575294
hantavirus-related acute interstitial nephritis in western europe. expansion of a world-wide zoonosis.we report the clinical, biological and serological characteristics of 76 cases of hantavirus-induced acute interstitial nephritis diagnosed in belgium and france between 1977 and 1986. the disease is characterized by a fever of sudden onset promptly accompanied by pain in the loin or abdomen, nausea and vomiting, myalgias and occasionally with acute myopia and conjunctival injection. within a week acute renal failure develops, necessitating haemo- or peritoneal dialysis in up to 10 per cent of p ...19892576470
hantavirus infections in humans and commensal rodents in determine the extent of hantavirus infection in singapore, serological studies using the indirect immunofluorescent antibody (ifa) test were conducted on commensal rodents and on human patients in 4 diagnostic groups. virus isolation using a vero e6 cell line was performed on hantaviral antigen-positive rodent lung tissue. of 142 rodents and 3 insectivores studied, 37 (26%) were seropositive for ifa. rattus norvegicus was the predominant species captured, with the highest species-specific ser ...19892575293
[differentiation of the type of ehf antibodies by rphi].this article reports the use of for differentiation of the type of ehf antibodies. a total of 58 convalescent-phase sera from ehf patients in natural foci of predominant apodemus type of ehf and in those of rattus type of efh were examined. in the former 34/36 (94.44%) belonged to apodemus type, and in the latter 22/22 (100%) belonged to rattus type. both rphi and hi obtained the same result in differentiation of the type of ehf antibodies. rphi possesses sensitivity and specificity in ehf diagn ...19892572325
[an electron microscopic study of viral infected peripheral mononuclear cells in epidemic hemorrhagic fever].ultrathin sections of peripheral blood cells from 7 patients of early stage epidemic hemorrhagic fever (ehf) were reexamined under transmission electron microscope. virus particles were found in the cytoplasmic vacuoles of mononuclear cells from two patients. the morphology and morphogenesis of the matured virus were identical with those of hantaan virus of the bunyaviridae family. additionally, abnormal aggregations of tubuloreticular structure (trs) were sometimes encountered in the dilated ci ...19892576865
efficacy of inactivated vaccine containing cyto-hemagglutinin against epidemic hemorrhagic fever in rabbits.using the z-10 strain of epidemic hemorrhagic fever virus (ehfv) as seed, and the primary cell of meriones unguiculatus kidney tissue as incubation cell, a propiolactone inactivated epidemic hemorrhagic fever (ehf) vaccine was prepared, according to a similar procedure required for the production of biological products such as the japanese b encephalitis vaccine. besides the ehfv antigen detected by elisa or reversed passive hemagglutination test (rpha) as were used for the formalin inactivated ...19892576866
[interruption of early viremia in patients with epidemic hemorrhagic fever].kinetic changes of viremia were observed in 287 patients with epidemic hemorrhagic fever (ehf) in whom ribavirin was administered with a double blind random controlled study by means of virus isolation, indirect immunofluorescence technique and elisa. the positive rate of viremia was 79.7%, and the positive rate of ehf igm was 85.0% before treatment. viremia could be interrupted by ribavirin. in the ribavirin group, the positive rate of viremia decreased, duration of viremia was shortened, viral ...19892576861
first sporadic case of hantavirus nephropathy, recognised in the netherlands. 19892576828
serologic survey of selected pathogens in white-tailed and mule deer in western nebraska.exposure of free-ranging white-tailed deer (odocoileus virginianus) and mule deer (odocoileus hemionus) in western nebraska to selected livestock pathogens was determined by serology and attempted virus isolation. antibodies to bluetongue virus, epizootic hemorrhagic disease virus, and bovine respiratory syncytial virus were present in both species of deer. no serologic reactors to brucella or anaplasma were found. attempts to isolate bluetongue virus were negative.19862845156
[endemic foci of hemorrhagic fever with renal syndrome in the north-western region of the european part of the ussr].for the detection of specific antigens of hemorrhagic fever with renal syndrome (hfrs) virus, examinations were carried out covering 8.061 animals of 23 species trapped in the murmansk, archangelsk, vologda, novgorod, pskov, leningrad regions, the komi assr, estonian ssr, latvian ssr, and lithuanian ssr in 1984-1987. different activity and variable species-specific composition of animal virus carriers in the north, south, and west of the north-western region were demonstrated. circulation of hfr ...19892576692
antiviral neutralizing antibody to hantaan virus as determined by plaque reduction technique.the 76--118 strain of hantaan virus was titrated in e6 (vero) cells by the plaque method using agarose overlay medium. visible plaques, formed 10 days post-infection, were uniformly 2--3 mm in diameter. dose-response experiments showed that a single infectious particle initiated the formation of a plaque. infectivity titers by the plaque method were equivalent to those obtained by the endpoint method (tcid50) using the immunofluorescence antibody technique (ifa) for antigen detection. the single ...19852859845
epidemiological study of hemorrhagic fever with renal syndrome related virus infection among urban rats in two islands in tokyo bay, japan.a seroepidemiological survey of hemorrhagic fever with renal syndrome (hfrs) virus infection in 403 urban rats (rattus norvegicus) captured on two different islands in tokyo bay was carried out using the indirect immunofluorescent antibody (ifa) test with the sr-11 strain of hfrs virus. antibody positive sera were detected in 99 out of 355 (27.9%) rats from the island with reclaimed ground area, but none were detected in 48 rats from the other islands. ifa titers of the positive sera varied from ...19852859764
[cases of hemorrhagic fever renal syndrome in 3 counties of the guang xi zhuang tribe autonomous region for the first time with the recovery of hemorrhagic fever viral agents from infected rodents]. 19852859926
[main reservoirs of infection of the hemorrhagic fever renal syndrome in endemic areas of anhui province]. 19852862997
[epidemiological study of epidemic hemorrhagic fever in china]. 19852862996
[hantaan viruses. pathogenic agents in zoonoses]. 19852861673
[hemorrhagic fever with renal syndrome in penza province].the evidence of the existence of natural foci of hfrs in penza region has first been presented on the basis of serological examinations of patients and normal population as well as hfrs virus antigen demonstration in the lung tissue of small mammals. an outbreak of hfrs among children in a pioneer camp is described. aerogenic infection of the children contracted from hfrs virus-infected bank voles (clethrionomys glareolus) living near the camp is assumed. the detection of hfrs antigen in 5 speci ...19852860753
[relationship between hosts and hemorrhagic fever renal syndrome in chang-an county, shan-xi province]. 19852862998
hantaan virus. 19852861238
serological and virological evidence of a hantaan virus-related enzootic in the united states.the first isolation of a hantaan-related virus from a feral rat in the united states was made from a rattus norvegicus caught in new orleans. the strain, designated tchoupitoulas virus, is antigenically related to, but distinct from, the prototype strain 76-118 of hantaan virus and is the first hantaan-like virus isolated from the pancreas of a naturally infected animal. serosurveys of wild rodents from urban and rural areas in the united states indicated that hantaan-related viruses infected ur ...19852861241
experimental infection with puumala virus, the etiologic agent of nephropathia epidemica, in bank voles (clethrionomys glareolus).subclinical chronic infections characterized by transient viremia, prolonged virus shedding in oropharyngeal secretions and feces, and virus persistence in tissues (particularly lung) developed in laboratory-bred weanling bank voles (clethrionomys glareolus) inoculated intramuscularly with puumala virus (strain hällnäs), the etiologic agent of nephropathia epidemica. viral antigen, as evidence by granular fluorescence, was detected in the lungs, liver, spleen, pancreas, salivary glands, and smal ...19852861296
hemorrhagic fever with renal syndrome: separation of human peripheral blood t and b cells and detection of viral lymphocytes in peripheral blood were prepared from patients with hemorrhagic fever with renal syndrome by a density gradient on ficoll-hypaque, and then t and b cells purified by passing the lymphocytes over a nylon column with modifications. also, after modification of cell fixation and use of a spinner for smear drying, the number of cells were increased and the duration for slide preparation was shortened, thus resulting in quality slides. detection of viral antigen by immunofluorescenc ...19892577361
isolation of a hantaan-related virus from brazilian rats and serologic evidence of its widespread distribution in south america.a serosurvey of domestic rats was conducted in several south american cities between september 1982 and march 1983 for evidence of hantavirus infection. antibody-positive rats were found in belem, são paulo and recife-olinda, brazil and in buenos aires, argentina, with the highest antibody prevalence rate detected in belem (30 positive of 54 tested, 56%). a virus isolated from tissues of a rattus norvegicus captured in belem, was shown to be antigenically similar to girard point viruses isolated ...19852862803
a scottish case of nephropathy due to hantaan virus illness characterised by acute renal failure, fever, conjunctivitis and a macular erythematous centrifugal rash is described in a scot who had not recently travelled abroad. there was serological evidence that the illness was due to hantaan or a closely related virus.19852863308
bunyaviridae. serological relationships.the family bunyaviridae comprises 5 genera of lipid-enveloped viruses with trisegmented rna genomes. one of the genera, bunyavirus, comprises over a quarter of the known arboviruses. the members of the hantavirus genus are apparently the exception in that they are not arthropod-borne. the genera are sorted largely on serological grounds. the nairoviruses, in addition to the presence of the physical marker of a higher molecular weight nucleoprotein, include congo-crimean haemorrhagic fever, an ap ...19892685515
the s rna segment of sandfly fever sicilian virus: evidence for an ambisense genome.the complete nucleotide sequence of the s rna segment of sandfly fever sicilian (sfs) virus (phlebovirus, bunyaviridae) was determined from overlapping cdna clones and by primer extension. the rna is 1746 nucleotides in length and has two large open reading frames (orf), one of which (24.8 kda) is viral-complementary in sense, and the other (30.4 kda) is in the viral sense. this ambisense genome arrangement has been seen in another member of the phlebovirus genus, punta toro (pt) virus (t. ihara ...19892705301
differential susceptibility and resistance of immunocompetent and immunodeficient mice to fatal hantaan virus infection.susceptibility and resistance to fatal hantaan virus meningoencephalitis were studied in immunocompetent (nu/+) and congenitally t-cell deficient (nu/nu) cd-1 mice of different ages. susceptibility of nu/+ mice to fatal infection was age-dependent, as evidenced by 100 percent mortality in mice inoculated intracerebrally with hantaan virus (strain 76-118) during the first week of life, 60 percent mortality in mice inoculated at 9 days of age, and no disease or death in mice inoculated at 14 to 42 ...19852864037
antigenic and genetic properties of viruses linked to hemorrhagic fever with renal syndrome.hemorrhagic fever with renal syndrome (hfrs) comprises a variety of clinically similar diseases of viral etiology that are endemic to and sporadically epidemic throughout the eurasian continent and japan. although hfrs has not been reported in north america, viruses that are antigenically similar to hfrs agents were recently isolated from rodents in the united states. examination and comparison of eight representative isolates from endemic disease areas and from regions with no known associated ...19852858126
outbreak of haemorrhagic fever with renal syndrome in outbreak of eight cases of haemorrhagic fever with renal syndrome in north-west greece is presented. the major clinical manifestation was fever and all patients subsequently developed decreased renal function and proteinuria. the disease was diagnosed by rising antibody titers to the hantaan virus.19852861091
isolation of haemorrhagic fever with renal syndrome virus from suncus murinus, an insectivore. 19852857871
serological evidence of hantaan virus infection in the united states.we found low titers of fluorescent antibodies against hantaan virus, the etiologic agent of korean hemorrhagic fever, in sera from 7 of 1,035 patients with febrile illnesses of unknown origin and from 6 of 664 blood donors in the united states. all but 1 of these individuals possessed neutralizing antibodies against hantaan virus. this was a 31-year-old research technician who had worked with laboratory rodents with virus-induced tumors, but had not traveled abroad, suggesting that infection wit ...19852858988
pathogenesis of hantaan virus infection in suckling mice: clinical, virologic, and serologic observations.hemorrhagic fever with renal syndrome (hfrs) is a debilitating disease of humans caused by hantaan virus (hv), the prototype member of a newly proposed genus of bunyaviridae. studies of hv pathogenesis have been limited by the absence of a well defined model for a virus-induced disease state. in an attempt to devise a model for hv pathogenesis in laboratory rodents, newborn outbred suckling icr mice were shown to be uniformly susceptible to lethal infection with non-mouse adapted hv by intracere ...19852858987
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