Publications

[hantavirus pulmonary syndrome in southern chile].		1999	10436725
isolation and genetic characterization of a hantavirus (bunyaviridae: hantavirus) from a rodent, oligoryzomys microtis (muridae), collected in northeastern peru.	this paper describes the isolation and partial genetic characterization of a hantavirus from a pygmy rice rat, oligoryzomys microtis, collected within the urban area of iquitos, loreto department, peru. the virus, designated htn-007, exhibited the highest degree of genetic similarity to rio mamore virus, which was originally described from the same rodent species in eastern bolivia. comparison of small and medium segment nucleotide sequence data from htn-007 and rio mamore virus revealed 87% and ...	1999	10432063
antibody responses in humans to an inactivated hantavirus vaccine (hantavax).	hantaviruses cause haemorrhagic fever with renal syndrome (hfrs) and result in severe human morbidity and mortality. safe and effective vaccines are needed urgently in order to reduce the incidence of human illness. hitherto studies of hantavirus vaccine efficiency have been limited to individuals at low risk of infection. in this study the immune response to an inactivated hantavirus vaccine was measured in 64 human volunteers at high risk of infection by virtue of residence and occupation. 30 ...	1999	10418904
hantavirus pulmonary syndrome (hps) in guariba, sp, brazil. report of 2 cases.	human infections caused by a hantavirus were reported in different regions of the state of são paulo (sp), brazil during the first six months of 1998. two cases of fatal pulmonary syndrome occurred in may of 1998 in the city of guariba, located in the northeastern region of sp. both patients worked in a corn storage barn infested by rodents. these patients, after 2 or 3 days of non-specific febrile illness, developed a severe interstitial pneumonia spreading widely in both lungs, causing respira ...	1999	10413962
[increased travelling--increased risk of imported viruses. a review of the diagnostic capacity of the swedish institute for infectious disease control].	the increase in global travel, especially by air, has facilitated the spread of infectious agents from one part of the world to another. returning travellers are often vectors for viral disease import to sweden. while dengue fever has been quite frequently diagnosed during the past five years, especially in travellers returning from thailand, only isolated cases of sandfly fever, japanese encephalitis and ross river fever have been seen. dobrava virus was recently shown to have caused a severe f ...	1999	10405531
update: hantavirus pulmonary syndrome--united states, 1999.	hantavirus pulmonary syndrome (hps) is a rodentborne viral disease characterized by severe pulmonary illness and a case-fatality ratio of 43%. sin nombre virus is the primary hantavirus that causes hps in the united states, and the deer mouse (peromyscus maniculatus) is its predominant carrier. cdc-sponsored studies of rodent populations since 1994 have yielded data that suggest an increased risk for infection for humans in some areas of the southwestern united states during the summer of 1999. ...	1999	10401910
pathogenesis of puumala and other hantavirus infections.	hantaviruses are rodent/insectivore-borne negative-stranded rna viruses which belong to the bunyaviridae family. they do not cause any symptomatic disease in their adult carrier rodents, but in humans they are aetiologic agents of haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps), both associated with a significant mortality. in cell culture hantaviruses do not cause cytopathic effects and the mechanisms of disease in man are not well understood. increased cap ...	1998	10398496
hantavirus in the americas.		1999	10382376
rodent control may help prevent human hantavirus infection		1999	10381750
risk factors for human hantavirus infection: franco-belgian collaborative case-control study during 1995-6 epidemic.		1999	10381709
[an outdoor person with high fever and flank pain].		1999	10380593
puumala virus infections in finland: increased occupational risk for farmers.	puumala hantavirus, transmitted by bank voles (clethrionomys glareolus), causes a mild-type hemorrhagic fever with renal syndrome. the disease is common in finland and is considered an occupational hazard for farmers, but the actual risk has not been assessed by analytical studies. data on 5,132 serologically confirmed puumala virus infections during 1989-1994 were analyzed, and cases among farmers and the population living in similar conditions were compared. the farmers contracted the disease ...	1999	10369509
isolation and characterization of a hantavirus from lemmus sibiricus: evidence for host switch during hantavirus evolution.	a novel hantavirus, first detected in siberian lemmings (lemmus sibiricus) collected near the topografov river in the taymyr peninsula, siberia (a. plyusnin et al., lancet 347:1835-1836, 1996), was isolated in vero e6 cells and in laboratory-bred norwegian lemmings (lemmus lemmus). the virus, named topografov virus (top), was most closely related to khabarovsk virus (kbr) and puumala viruses (puu). in a cross focus reduction neutralization test, anti-top lemmus antisera showed titers at least fo ...	1999	10364307
human memory cytotoxic t-lymphocyte (ctl) responses to hantaan virus infection: identification of virus-specific and cross-reactive cd8(+) ctl epitopes on nucleocapsid protein.	hantaan virus, the prototypic member of the hantavirus genus, causes hemorrhagic fever with renal syndrome in humans. we examined the human memory t-lymphocyte responses of three donors who had previous laboratory-acquired infections with hantaan virus. we demonstrated virus-specific responses in bulk cultures of peripheral blood mononuclear cells (pbmc) from all donors. bulk t-cell responses were directed against either hantaan virus nucleocapsid (n) or g1 protein, and these responses varied be ...	1999	10364276
were hantaviruses eventually responsible for the lost anasazi culture?		1998	10358738
[new aspects of the pathogenesis of hantavirus infections. a systemic disease with general vascular changes].		1999	10354671
genetic evidence of dobrava virus in apodemus agrarius in hungary.	using nested polymerase chain reaction, we sequenced dobrava virus (dob) from the rodent apodemus agrarius in hungary. the samples we isolated group with dob samples previously isolated from a. flavicollis. this grouping may indicate host switching.	1999	10341190
chronic renal dysfunction after hantavirus infection.		1999	10328510
hantavirus nephropathy in a child.		1999	10328509
new world hantaviruses.	since the initial description in 1993 of hantavirus pulmonary syndrome and its novel aetiological agent, sin nombre virus, our knowledge of the epidemiology of new world hantaviruses has continued to evolve. after the identifying outbreak in the southwestern us, four hantaviruses have been identified in north america with specific rodent hosts and associated with a number of sporadic cases. this stability of case recognition in north america is in contrast to the multiple outbreaks and endemic c ...	1998	10326292
low prevalence of antibodies against the zoonotic agents brucella abortus, leptospira spp., streptococcus suis serotype ii, hantavirus, and lymphocytic choriomeningitis virus among veterinarians and pig farmers in the southern part of the netherlands.	serum samples from 102 veterinarians and 191 pig farmers from the southern part of the netherlands were investigated for antibodies against brucella abortus, leptospira spp, streptococcus suis serotype ii, hantavirus (hv), and lymphocytic choriomeningitis virus (lcmv). all samples were collected in 1993 and stored until this study was performed. the prevalence of antibodies against b.abortus in veterinarians (4.5%) was significantly higher (p = 0.01) than in pig farmers (0%). none of the veterin ...	1999	10321013
emerging infectious diseases and pathogens.	emerging infectious diseases are caused by old, new, and mutant microorganisms. emergence of these pathogens can be attributed to changes in the characteristics and risk factors of patients, the widespread use of antibiotics, changes in the environment, the role of xenotransplantation, and international travel. in the united states, the incidences of c. difficile, cyclosporiasis, enterohemorrhagic e. coli gastroenteritis, hantavirus, hepatitis c virus infection, and lyme disease have increased s ...	1999	10318733
relationships of deer mouse movement, vegetative structure, and prevalence of infection with sin nombre virus.	the effects of vegetative structure on movement of deer mice (peromyscus maniculatus) were examined in two distinct vegetation associations, one near hesperus and the other near molina in western colorado (usa) from june-october 1994 to october 1998. we monitored movement by live-trapping small mammals within gambel's oak/mixed-grass (hesperus) and sage brush/juniper (molina) vegetation types. vegetative structure differed between the sites with molina having more cover provided by shrubs and he ...	1999	10231758
polymerase chain reaction detection of puumala virus rna in formaldehyde-fixed biopsy material.	infections with hantaviruses, mainly clethrionomys-derived puumala viruses, are known causes of acute renal failure [hemorrhagic fever with renal syndrome (hfrs)] in western europe. laboratory diagnosis is primarily based on serology. at the time of clinical symptoms, viral rna can hardly be detected in the blood or urine, indicating that polymerase chain reaction (pcr) is of little diagnostic value for these infections. biopsy material is usually formaldehyde-fixed and, thus, regarded as poor q ...	1999	10231472
hbv core particles allow the insertion and surface exposure of the entire potentially protective region of puumala hantavirus nucleocapsid protein.	core particles of the hepatitis b virus (hbv) potentiate the immune response against foreign epitopes presented on their surface. potential insertion sites in the monomeric subunit of the hbv core protein were previously identified at the n- and c-terminus and in the immunodominant c/e1 region. in a c-terminally truncated core protein these sites were used to introduce the entire 120 amino acid (aa)-long potentially immunoprotective region of the hantavirus (serotype puumala) nucleocapsid protei ...	1999	10223335
cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins.	hantaviruses replicate primarily in the vascular endothelium and cause two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). in this report, we demonstrate that the cellular entry of hfrs-associated hantaviruses is facilitated by specific integrins expressed on platelets, endothelial cells, and macrophages. infection of human umbilical vein endothelial cells and vero e6 cells by the hfrs-causing hantaviruses hantaan (htn), seoul (seo), and puum ...	1999	10196290
hantavirus-specific igg, igm, and iga in acute and chronic renal disease versus congenital renal disease in the united states.	in a blinded fashion, 165 serum samples from patients with biopsy-characterized acute and chronic renal diseases (acrds), 34 serum samples from patients with congenital renal diseases (crds), and 100 serum samples from healthy adults were assayed for immunoglobulin g (igg), igm, and iga antibodies to hantaan and puumala viruses by enzyme immunoassay (eia). twenty-six percent (44 of 165) of acrds, 3% (1 of 34) of crds, and none (0 of 100) of the healthy serum samples were positive for hantavirus- ...	1999	10196017
enhancing laboratory detection of disease. part 3: detecting a hantavirus disease event: a case study.	a purposive syndromic classification diagnostic system, with known means to ends, has been developed that extracts bits of structural information from endogenous laboratory data. an edifice of structural information for organization, regulation, and decision-making was built for determining future pathophysiological events by asking the fewest questions to define the next event uniquely. the structural information is based on laboratory ordering patterns of physicians for patient populations the ...	1997	10173024
enhancing laboratory detection of disease. part 2: amplifying information and requisite variety.		1997	10172959
plagues. guerilla warfare.		1996	10161475
new and emerging pathogens, part 4. new and emerging viral diseases--the ultimate parasites.		1996	10157594
hantaviruses: an overview and radiographic correlation.	this article describes hantaviruses and the infections they cause. routes of transmission, disease phases and radiographic manifestations are discussed, along with recommendations for prevention and the radiologic technologist's role in prompt diagnosis.	1999	10101753
[hantavirus and pulmonary pathology].	the hantavirus pulmonary syndrome recognized in the united states in 1993 quickly brought the formerly little known virus into the limelight. contrary to what has been written almost everywhere, this is not a "new" virus causing new "emerging" disease. hantaviruses have been harbored in their natural hosts, three subfamilies of murine rodents, for millions of years. the phylogenetic classifications of these viruses follows that of their hosts, proving close adaptation and contradicting short-ter ...	1998	10100354
maternal transfer of hantavirus antibodies in rats.		1998	10090051
[hantavirus infection].		1999	10088345
genetic diversities of hantaviruses among rodents in hokkaido, japan and far east russia.	seroepizootiologic surveys among wild rodents were carried out in japan and far east russia in 1995 and 1996. seropositive animals were only identified in clethrionomys rufocanus (23/134) in hokkaido, japan. on the other hand, seropositives were identified in c. rufocanus (1/8), apodemus agrarius (2/66), apodemus spp. (2/26) and microtus fortis (3/22) in vladivostok, far east russia. total rna was isolated from lungs of seropositive animals and the s genome segments were amplified by pcr, cloned ...	1999	10082393
navigational instinct: a reason not to live trap deer mice in residences.		1999	10081690
a midcourse assessment of hantavirus pulmonary syndrome.		1999	10081689
long-term studies of hantavirus reservoir populations in the southwestern united states: a synthesis.	a series of intensive, longitudinal, mark-recapture studies of hantavirus infection dynamics in reservoir populations in the southwestern united states indicates consistent patterns as well as important differences among sites and host-virus associations. all studies found a higher prevalence of infection in older (particularly male) mice; one study associated wounds with seropositivity. these findings are consistent with horizontal transmission and transmission through fighting between adult ma ...	1999	10081681
natural history of sin nombre virus in western colorado.	a mark-recapture longitudinal study of immunoglobulin g (igg) antibody to sin nombre virus (snv) in rodent populations in western colorado (1994-results summarized to october 1997) indicates the presence of snv or a closely related hantavirus at two sites. most rodents (principally deer mice, peromyscus maniculatus, and pinyon mice, p. truei) did not persist on the trapping webs much beyond 1 month after first capture. some persisted more than 1 year, which suggests that even a few infected deer ...	1999	10081680
statistical sensitivity for detection of spatial and temporal patterns in rodent population densities.	a long-term monitoring program begun 1 year after the epidemic of hantavirus pulmonary syndrome in the u.s. southwest tracked rodent density changes through time and among sites and related these changes to hantavirus infection rates in various small-mammal reservoir species and human disease outbreaks. we assessed the statistical sensitivity of the program's field design and tested for potential biases in population estimates due to unintended deaths of rodents. analyzing data from two sites in ...	1999	10081679
a longitudinal study of sin nombre virus prevalence in rodents, southeastern arizona.	we determined the prevalence of sin nombre virus antibodies in small mammals in southeastern arizona. of 1,234 rodents (from 13 species) captured each month from may through december 1995, only mice in the genus peromyscus were seropositive. antibody prevalence was 14.3% in 21 white-footed mice (p. leucopus), 13.3% in 98 brush mice (p. boylii), 0.8% in 118 cactus mice (p. eremicus), and 0% in 2 deer mice (p. maniculatus). most antibody-positive mice were adult male peromyscus captured close to o ...	1999	10081678
long-term hantavirus persistence in rodent populations in central arizona.	for 35 months, we monitored hantavirus activity in rodent populations in central arizona. the most frequently captured hantavirus antibody-positive rodents were peromyscus boylii and p. truei. antibody-positive p. boylii were more frequently male (84%), older, and heavier, and they survived longer on trapping web sites than antibody-negative mice. the number of antibody-positive p. boylii was greater during high population densities than during low densities, while antibody prevalence was greate ...	1999	10081677
long-term studies of hantavirus reservoir populations in the southwestern united states: rationale, potential, and methods.	hantaviruses are rodent-borne zoonotic agents that cause hemorrhagic fever with renal syndrome in asia and europe and hantavirus pulmonary syndrome (hps) in north and south america. the epidemiology of human diseases caused by these viruses is tied to the ecology of the rodent hosts, and effective control and prevention relies on a through understanding of host ecology. after the 1993 hps outbreak in the southwestern united states, the centers for disease control and prevention initiated long-te ...	1999	10081676
climatic and environmental patterns associated with hantavirus pulmonary syndrome, four corners region, united states.	to investigate climatic, spatial, temporal, and environmental patterns associated with hantavirus pulmonary syndrome (hps) cases in the four corners region, we collected exposure site data for hps cases that occurred in 1993 to 1995. cases clustered seasonally and temporally by biome type and geographic location, and exposure sites were most often found in pinyon-juniper woodlands, grasslands, and great basin desert scrub lands, at elevations of 1,800 m to 2,500 m. environmental factors (e.g., t ...	1999	10081675
genetic diversity and distribution of peromyscus-borne hantaviruses in north america.	the 1993 outbreak of hantavirus pulmonary syndrome (hps) in the southwestern united states was associated with sin nombre virus, a rodent-borne hantavirus; the virus' primary reservoir is the deer mouse (peromyscus maniculatus). hantavirus-infected rodents were identified in various regions of north america. an extensive nucleotide sequence database of an 139 bp fragment amplified from virus m genomic segments was generated. phylogenetic analysis confirmed that snv-like hantaviruses are widely d ...	1999	10081674
[acute hantavirus infection caused by a genetically newly identified viral strain. severe and complicated course of hemorrhagic fever with renal syndrome (nephropathia epidemica)].	a 49-year-old patient, a hobby hunter, fell ill acutely with joint and limb pain, abdominal pain, nausea and subfebrile temperatures. at hospitalization, the patient was in bad general condition, showing ascites and lid edema, and acute renal failure was diagnosed.	1999	10081288
emerging viral infections.		1999	10079847
isolation and characterization of dobrava hantavirus carried by the striped field mouse (apodemus agrarius) in estonia.	dobrava hantavirus (dob) was isolated from the striped field mouse (apodemus agrarius) trapped on saaremaa island, estonia, and its genetic and antigenic characteristics were subsequently analysed. phylogenetic analysis showed that the estonian dob strain, together with several wild strains carried by apodemus agrarius, forms a well-supported lineage within the dob clade. the topography of the trees calculated for the s, m and l nucleotide sequences of the estonian dob suggests a similar evoluti ...	1999	10073697
spectrum of hantavirus infection: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome.	hantaviruses chronically infect rodents without apparent disease, but when they are spread by aerosolized excreta to humans, two major clinical syndromes result: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). both diseases appear to be immunopathologic, and inflammatory mediators are important in causing the clinical manifestations. in hps, t cells act on heavily infected pulmonary endothelium, and it is suspected that gamma interferon and tumor necrosis fa ...	1999	10073292
hantavirus pulmonary syndrome.	we present the first case of hantavirus pulmonary syndrome in the dallas/fort worth area. the patient was a 50-year-old previously healthy white man, who had adult respiratory distress syndrome (ards) and hypotensive shock after 1 week of nonspecific "viral" symptoms. despite supportive care, the patient died within several hours of presentation. this case illustrates several of the classic hallmarks of hantavirus infection such as hemoconcentration, thrombocytopenia, ards, and shock.	1999	10071675
dna vaccination with hantavirus m segment elicits neutralizing antibodies and protects against seoul virus infection.	seoul virus (seov) is one of four known hantaviruses causing hemorrhagic fever with renal syndrome (hfrs). candidate naked dna vaccines for hfrs were constructed by subcloning cdna representing the medium (m; encoding the g1 and g2 glycoproteins) or small (s; encoding the nucleocapsid protein) genome segment of seov into the dna expression vector pwrg7077. we vaccinated balb/c mice with three doses of the m or s dna vaccine at 4-week intervals by either gene gun inoculation of the epidermis or n ...	1999	10069952
high-producer allele of tumour necrosis factor-alpha is part of the susceptibility mhc haplotype in severe puumala virus-induced nephropathia epidemica.	the biallelic polymorphisms in the tumour necrosis factor-alpha (tnf-alpha) gene promoter region were studied in patients with nephropathia epidemica (ne). the rarer tnf2 allele, associated with a high tnf-alpha producer phenotype, was more frequently found in hospitalized ne patients (42%) than in healthy controls (15%; p=0.0064). the putative role of tnf2 as an independent risk factor for ne is discussed.	1998	10066063
first case of infection with hantavirus dobrava in germany.		1998	10052557
hantavirus (bunyaviridae) infections in rodents from orange and san diego counties, california.	during a screening program to determine the extent of hantavirus activity in orange and san diego counties, california, serum samples from 2,365 rodents representing nine genera and 15 species were tested for hantavirus antibodies. a reverse transcription-polymerase chain reaction on selected seropositive rodents was used to identify the specific hantavirus. rodents positive for sin nombre virus (snv) antibodies by western blot included 86 (9.1%) of 948 deer mice (peromyscus maniculatus), four ( ...	1999	9988327
dobrava hantavirus in russia.		1999	9923881
["emerging" viral zoonoses in native animals who can be the host in the netherlands].	viral zoonotic diseases can be defined as 'viral diseases transmitted from vertebrate animals to man'. viral zoonoses have been emerging and re-emerging throughout history and can cause serious diseases and disease outbreaks in human populations. this paper deals with emerging viral zoonotic infections that have hosts in native species and can be a hazard for the human population in the netherlands. rabies virus, hantavirus, influenza virus tick-borne encephalitis virus, borna disease virus and ...	1999	9921612
[diseases caused by hantaviruses].	hantaviruses are rna viruses that belong to the genus hantavirus, family bunyaviridae. the main reservoir of hantaviruses are rodents. humans become infected by inhaling particles of dust contaminated with faeces of rodents, that are found in europe and asia hantaviruses named: hantaan, seoul and dobrava-belgrade causing haemorrhagic fever with renal syndrome (hfrs) are prevalent. typical clinical picture of hfrs consists of renal insufficiency (primarily anuria and then polyuria with disturbanc ...	1998	9919918
[hantaviruses--a global problem].		1998	9885644
development of humoral cross-reactivity to the nucleocapsid protein of heterologous hantaviruses in nephropathia epidemica.	a hantavirus infection is followed by a prominent antibody response to the viral nucleocapsid protein. antibodies from patients infected with one hantavirus cross-react to varying degrees with the nucleocapsid protein of other viruses of the genus. we studied the cross-reactivity in serially obtained blood samples from 17 patients with nephropathia epidemica, a european form of hemorrhagic fever with renal syndrome caused by puumala virus. recombinant truncated nucleocapsid protein (aa 1-117) of ...	1998	9879922
high levels of cytokine-producing cells in the lung tissues of patients with fatal hantavirus pulmonary syndrome.	hantavirus pulmonary syndrome (hps) is characterized by the rapid onset of pulmonary edema and a high case-fatality rate. hantavirus antigens have been demonstrated in pulmonary capillary endothelial cells, but the mechanisms causing capillary leakage remain unclear. immunohistochemical staining was used to enumerate cytokine-producing cells (monokines: interleukin [il]-1alpha, il-1beta, il-6, and tumor necrosis factor [tnf]-alpha; lymphokines: interferon-gamma, il-2, il-4, and tnf-beta) in tiss ...	1999	9878011
the muskrat (ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in europe.	we have used an indirect immunofluorescent assay (ifa) and reverse transcription-pcr (rt-pcr) to screen the sera and tissues of muskrats (ondatra zibethica) caught in the northwest of brandenburg and in the northeast of saxony-anhalt, germany, for hantavirus infection. kidney and/or lung tissue from 6 (3.1%, ci = 1.1-6.5%) out of 197 muskrats were found to be positive for genomic sequences of hantavirus by rt-pcr. we could also demonstrate that 14 (5%, ci = 2.9-8.7%) out of 266 muskrat's sera av ...	1998	9870582
assessment of person-to-person transmission of hantavirus pulmonary syndrome in a chilean hospital setting.	person-to-person transmission of andes hantavirus among healthcare workers was reported in argentina for the first time in 1996. to determine whether such transmission of the virus occurred during a 1997 outbreak of hantavirus pulmonary syndrome (hps) in southern chile due to andes virus, we conducted a serological and epidemiological study in the coyhaique regional hospital, where the majority of hps patients were admitted. workers in every department of the hospital were evaluated for immunogl ...	1998	9868619
update on emerging infections from the centers for disease control and prevention. hantavirus pulmonary syndrome--colorado and new mexico, 1998.		1999	9867901
an outbreak of hantavirus pulmonary syndrome, chile, 1997.	an outbreak of 25 cases of andes virus-associated hantavirus pulmonary syndrome (hps) was recognized in southern chile from july 1997 through january 1998. in addition to the hps patients, three persons with mild hantaviral disease and one person with asymptomatic acute infection were identified. epidemiologic studies suggested person-to-person transmission in two of three family clusters. ecologic studies showed very high densities of several species of sigmodontine rodents in the area.	1998	9866751
ecologic studies of rodent reservoirs: their relevance for human health.	within the past few years, the number of "new" human diseases associated with small-mammal reservoirs has increased dramatically, stimulating renewed interest in reservoir ecology research. a consistent, integrative approach to such research allows direct comparisons between studies, contributes to the efficient use of resources and data, and increases investigator safety. we outline steps directed toward understanding vertebrate host ecology as it relates to human disease and illustrate the rel ...	1998	9866729
stable expression of nucleocapsid proteins of puumala and hantaan virus in mammalian cells.	the development of an in vitro-system for the stable expression and the analysis of native hantavirus proteins using hantaviral cdna is of particular interest. as a first step the expression of the hantavirus nucleocapsid (n) proteins in mammalian cells was studied in more detail. the cdna of the s-rna segment of puumala virus strain cg-1820 and hantaan virus strain 76-118 was used for the construction of eucaryotic expression vectors that allow the generation and selection of mammalian cells ha ...	1998	9857992
new york 1 and sin nombre viruses are serotypically distinct viruses associated with hantavirus pulmonary syndrome.	new york 1 virus (ny-1) and sin nombre virus (sn) are associated with hantavirus pulmonary syndrome (hps). ny-1 and sn are derived from unique mammalian hosts and geographic locations but have similar g1 and g2 surface proteins (93 and 97% identical, respectively). focus reduction neutralization assays were used to define the serotypic relationship between ny-1 and sn. sera from ny-1-positive peromyscus leucopus neutralized ny-1 and sn at titers of >/=1/3,200 and </=1/400, respectively (n = 12). ...	1999	9854075
recombination in tula hantavirus evolution: analysis of genetic lineages from slovakia.	to examine the evolution of tula hantavirus (tul), carried by the european common vole (microtus arvalis and m. rossiaemeridionalis), we have analyzed genetic variants from slovakia, the country where the virus is endemic. phylogenetic analysis (phylip) based on either partial (nucleotides [nt] 441 to 898) or complete n-protein-encoding sequences divided slovakian tul variants into two main lineages: (i) strains from eastern slovakia, which clustered with russian strains, and (ii) strains from w ...	1999	9847372
sin nombre virus pathogenesis in peromyscus maniculatus.	sin nombre virus (snv), a member of the hantavirus genus, causes acute viral pneumonia in humans and is thought to persistently infect mice. the deer mouse, peromyscus maniculatus, has been identified as the primary reservoir host for snv. to understand snv infection of p. maniculatus, we examined wild deer mice for localization of viral antigens and nucleic acid. morphologic examination consistently revealed septal edema within lung tissue and mononuclear cell infiltrates in portal areas of the ...	1999	9847363
black creek canal virus infection in sigmodon hispidus in southern florida.	a total of 1,500 small mammals were collected and tested for antibodies cross-reactive to sin nombre virus (hantavirus: bunyaviridae) at 89 sites in a 1,600 km2 study area of southern florida. more than 95% of the 123 seropositive animals were cotton rats (sigmodon hispidus), suggesting infection by black creek canal virus, although seroreactive rattus rattus (5 of 294) and peromyscus gossypinus (1 of 39) also were captured. crude seroprevalence in s. hispidus was 11%. seroprevalence increased w ...	1998	9840584
hantavirus infection--new threats by an old virus.		1998	9829468
epidemiology of hantavirus infections in europe.		1998	9829467
a minority of seropositive wild bank voles (clethrionomys glareolus) show evidence of current puumala virus infection.	bank voles (clethrionomys glareolus) serve as the reservoir for puumala (puu) virus, the aetiologic agent of nephropathia epidemica. the animals are believed to be persistently infected and the occurrence of serum antibodies is usually taken as an evidence of active infection. we found serum antibodies to puu virus in 42 of 299 wild bank voles captured in a puu virus endemic area. puu virus rna was demonstrated in lung specimens of 11 of these 42 animals and in 2 of them antigen was also found. ...	1998	9825795
completion of the tula hantavirus genome sequence: properties of the l segment and heterogeneity found in the 3' termini of s and l genome rnas.	in this study the l segment and the 5' and 3' termini of the s, m and l segments of the prototype tula hantavirus (tul) were sequenced, thus completing the first determination of the genome sequence of a hantavirus that has not been linked to any human disease. the tul l segment comprises 6541 nt with one orf of 6459 nt in the antigenome sense. this orf potentially encodes a 2153 aa protein with a predicted molecular mass of 247 kda. the amino acid sequence includes all the motifs conserved in r ...	1998	9820136
isolation and characterization of puumala hantavirus from norway: evidence for a distinct phylogenetic sublineage.	puumala (puu) hantavirus is the aetiological agent of nephropathia epidemica (ne), a mild form of haemorrhagic fever with renal syndrome, which occurs in fennoscandia, central europe and russia. in norway, ne-like disease has been reported since 1946 and about 50 cases are diagnosed annually; however, the causative agent has not been characterized. in this study, a virus originating from bank voles (clethrionomys glareolus) trapped near the town of eidsvoll (akershus county) was isolated and pas ...	1998	9820135
hantavirus infection in the four corners region of usa in 1998.		1998	9802275
seroprevalence of antibodies to hantavirus in health care workers and other residents of southern argentina.		1998	9798052
a major outbreak of hemorrhagic fever with renal syndrome in the samara region, european russia.		1998	9795799
[anti-hantavirus antibodies in school children in salvador, bahia].	hantavirosis are emerging diseases in the americas. although considered rare in children, among the five cases diagnosed in brazil, one was in this age group. to know the serum-prevalence of hantavirosis in the pediatric group (schoolchildren) of low social economic level in salvador, bahia state, and to associate demographic, socio-economic and environmental factors to the serologic results, a standard questionnaire was applied and blood samples were collected from 379 students of two public sc ...	1998	9789441
nucleotide sequence and phylogenetic analysis of the medium (m) genomic rna segments of three hantaviruses isolated in china.	the medium (m) genome segment of hantaviruses (family bunyaviridae) encodes the two virion glycoproteins. g1 and g2, as a precursor protein in the complementary sense rna. we determined the nucleotide sequences of the m genome segments of three chinese hantavirus isolates, a hantaan-type (htn) virus designated a9 and two seoul-type (seo) viruses designated l99 and hb55, and compared them to those of other htn or seo viruses isolated in eastern asia. the m segment of a9 is 3616 nucleotides in len ...	1998	9784066
infectious agents and the etiology of chronic idiopathic diseases.	at the end of the nineteenth century, the field of microbiology was born, and the infectious nature of many previously unexplained diseases was illuminated as powerful new technology was applied. at the end of the twentieth century, the etiology of myriad chronic diseases remains unexplained. we have argued that many of these diseases have clinical, epidemiological, and pathological features that suggest a role for microbes in their pathogenesis. although definitive evidence of microbial disease ...	1998	9779355
[in the month of may, do whatever you please...].		1998	9775088
hantavirus seropositivity in israeli patients with renal failure.	the hantavirus is known to cause hemorraghic fever with renal syndrome (hfrs), which is widely spread in europe and asia. several reports have shown an association of hantavirus antibody titers and the occurrence of renal dysfunction. from these observations, it appears that the virus is widely distributed, and different strains prevail in various areas. in the present work we studied 81 patients with end-stage renal-failure under hemodialysis (hd) treatment, 55 with mild to moderate renal failu ...	1998	9765032
first reported case of hantavirus pulmonary syndrome in oklahoma.	hantavirus pulmonary syndrome (hps) is a condition of rapidly progressive pulmonary failure with a case-fatality rate of almost 50 percent. rodents serve as the reservoir for hantaviruses and human infection occurs primarily via aerosolized virus in rodent excreta. the rodent reservoir for the disease is widespread across oklahoma and the first case of hps has recently been confirmed in an oklahoma resident. physicians should suspect hps in a previously healthy person who develops a febrile illn ...	1998	9763765
ecology and demographics of hantavirus infections in rodent populations in the walker river basin of nevada and california.	to study the ecologic correlates of hantavirus in deer mice (peromyscus maniculatus), we sampled 114 sites in the walker river basin of nevada and california in 1995-1996. blood samples were tested for antibody to hantavirus, and a subset of samples was also tested for virus rna by reverse transcription-polymerase chain reaction. average prevalence of antibody-positive mice was 17%, with heavier males the most likely to be infected. antibody prevalence varied within repeatedly sampled sites from ...	1998	9749642
high prevalence of hantavirus infection in indian communities of the paraguayan and argentinean gran chaco.	serologic evidence of past infection with a sin nombre-like hantavirus(es) was demonstrated in 78 (40.4%) of 193 indians living in western paraguay and in 38 (17.1%) of 222 indians inhabiting the salta province of northern argentina. in both populations seroprevalence increased with age, with the most striking increase occurring at 18 years of age in the paraguayan population and at 35 years of age in the salta population. the peak prevalences in both populations (66.6% and 44.0%, respectively) ...	1998	9749641
hantaviruses: an emerging threat to human health?		1998	9742991
zoonoses and haemorrhagic fever.	virus zoonoses causing haemorrhagic fever have been recognized in three major families: arenaviridae, bunyaviridae and filoviridae. all are negative-stranded rna viruses, with genomes in two segments, three segments, or non-segmented, respectively. acquisition of haemorrhagic fever in man generally requires close contact with a vertebrate vector species, usually rodents, for the arenaviruses and bunyaviruses. in the case of filoviruses, the vector is currently unknown, but these viruses may infe ...	1998	9737374
lack of serologic evidence for an association between hantavirus infection and end-stage renal disease.		1998	9730717
hantavirus pulmonary syndrome treated with inhaled nitric oxide.		1998	9726354
isolated abducens nerve palsy as a complication of haemorrhagic fever with renal syndrome.		1998	9719177
[america hantaviruses: new data and implications].		1998	9718552
human dobrava hantavirus infections in estonia.	outbreaks of severe hemorrhagic fever with renal syndrome (hfrs) caused by dobrava hantavirus (dob) have been reported in albania, greece, and bosnia-herzegovina. a seroepidemiologic study conducted by the authors in estonia provided the first evidence of human dob infection in a baltic country. hantavirus infection was first confirmed in estonia in 1986 and believed related to puumala hantavirus (puu). to enable precise serotyping, serum samples were collected from 1728 healthy people in 11 ...	1998	9717928
host genes and infectious diseases.		1998	9716963
southwest cautious over hantavirus outbreak.		1998	9713524
from the centers for disease control and prevention. hantavirus pulmonary syndrome--colorado and new mexico, 1998.		1998	9707130
antigenic characterization of hantaan and seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen.	hantaan virus (htn) and seoul virus (seo) are members of the genus hantavirus in the family bunyaviridae and are causative agents of hemorrhagic fever with renal syndrome. the complete and truncated nucleocapsid proteins (np) of htn and seo were expressed by a recombinant baculovirus system. antigenic characterization of the np using monoclonal antibodies (mabs) indicated that the binding sites for the serotype-specific mabs were located between amino acids (aa) 155 and 429. a western blot assay ...	1998	9705385
characterization of the nucleic acid binding properties of tomato spotted wilt virus nucleocapsid protein.	tomato spotted wilt tospovirus (tswv) is the type member of the plant-infecting viruses of the genus tospovirus in the family bunyaviridae. the three tswv rnas are encapsidated with nucleocapsid (n) protein to form ribonucleoprotein (rnp) which serves as the template for viral transcription and replication. regions of the open reading frame coding for the n protein on the small (s) rna were subcloned into pet protein expression vectors and expressed in escherichia coli bl21 (de3) cells. full-len ...	1998	9705250
automatic detection of conserved rna structure elements in complete rna virus genomes.	we propose a new method for detecting conserved rna secondary structures in a family of related rna sequences. our method is based on a combination of thermodynamic structure prediction and phylogenetic comparison. in contrast to purely phylogenetic methods, our algorithm can be used for small data sets of approximately 10 sequences, efficiently exploiting the information contained in the sequence variability. the procedure constructs a prediction only for those parts of sequences that are consi ...	1998	9685502