Publications

[an epidemic of hemorrhagic fever with renal syndrome at the therapeutic community for treatment of psychoactive drug dependence in ivanovac].	the outbreak of hemorrhagic fever with renal syndrome (hfrs) at the ivanovac drug therapeutic community near nova gradiska was analyzed. the major epidemiologic, clinical and serologic findings are presented.	2003	15011461
[is permanent renal function damage possible after hemorrhagic fever with renal syndrome?].	to investigate the possibility of permanent renal function impairment and other organ lesions following hemorrhagic fever with renal syndrome (hfrs).	2003	15011462
[puumala and dobrava viruses in the northeastern and central regions of bosnia].	bosnia and herzegovina has been known as a highly endemic region for hantavirus infections for more than 50 years. previous studies have shown that at least two different hantaviruses, the murine dobrava (dob) and avricoline puumala (puu) viruses, each carried by a different rodent species, have been circulating in the area. however, there is little information on rodent population density fluctuations in bosnia over the past years as well as on the ratio of puumala to dobrava infection in human ...	2003	15011464
[serologic diagnosis of hemorrhagic fever with renal syndrome in bosnia and herzegovina in 2002].	results of the serologic confirmation of hemorrhagic fever with renal syndrome (hfrs) in patients with clinical picture and overt symptoms of the disease from particular areas in bosnia and herzegovina during 2002 are presented.	2003	15011465
[what is new in the epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia?].	the aim was to describe epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia.	2003	15011468
[immunopathogenesis of hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome].	hantaviruses (htv) cause two serious human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps), posing a considerable public health problem worldwide. immunopathogenesis has been suggested to be involved in both hfrs and hps. a common feature of hantavirus diseases is an increased microvascular bed permeability, suggesting that vascular endothelium is a prime target of virus infection. although htv infect numerous cell lines and primary cells, it was co ...	2003	15011469
[ocular changes in hemorrhagic fever with renal syndrome].	hemorrhagic fever with renal syndrome (hfrs) is a rodent-originating zoonotic disease caused by puumala and dobrava viruses, which belong to the genus hantavirus. the most prominent ocular change in hfrs is transient myopia, which lasts for up to one week and is due to a forward movement of the ocular anterior diaphragm and thickening of the lens. other ophthalmic manifestations of hfrs are eyelid edema, conjunctival injection with or without hemorrhages, chemosis, anterior uveitis, retinal edem ...	2003	15011470
[hemorrhagic fever with renal syndrome: imported or native disease in the zadar region].	the aim of the study is to warn of the need of clinical hemorrhagic fever with renal syndrome (hfrs) recognition and determination, and of the risk of its spread from a new focus of this natural zoonosis near zadar (north dalmatia), i.e., in the northern part (lika) of the zadar county.	2003	15011474
the seroprevalences of anti-hantavirus igg antibodies among selected venezuelan populations.	in venezuela, the isolation of hantaviruses from rodents and the detection, in 1999, of a clinically confirmed human case of hantavirus infection led to increased interest in these viruses. in an attempt to estimate the problem posed by such viruses in venezuela, elisa based on purified, recombinant, nucleoprotein were used to check 1380 human serum samples for the presence of igg antibodies to hantavirus. the elisa results, as confirmed by indirect immunofluorescence and western-blot assays, in ...	2003	12662423
hantaviruses: a tale of two hemispheres.	infectious febrile nephropathies, initially described as epidemics during military campaigns in europe and asia at the turn of the 20(th) century, were eventually all unified under the rubric of hemorrhagic fever with renal syndrome (hfrs) and ascribed to infection from rodent-borne hantaviruses. at the end of the century, hantavirus pulmonary syndrome (hps) was described throughout the americas as a consequence of infection with the new world hantaviruses. the epidemiology and clinical manifest ...	2003	12682619
viral zoonoses - a threat under control?	despite intensive research and considerable effort to eradicate infectious diseases, modern medicine has failed to control many infectious diseases which have been thought to be easy to overcome with advances in medical science and technology. in fact, infectious diseases remain a dominant feature in public health considerations for the 21st century. some infectious agents already known to be pathogenic have gained increasing importance in recent decades due to changes in disease patterns. furth ...	2003	12684545
hantavirus infection in an active duty u.s. army soldier stationed in seoul, korea.	hemorrhagic fever with renal syndrome has been described among the u.s. and the u.n. troops assigned to the republic of korea since the korean war. however, this appears to be the first case in which infection was acquired during garrison duty in an urban environment.	2003	12685690
[new data for the public health importance of hantaviruses in hungary].	the authors present recent results in the hungarian hantavirus ecology and epidemiology. most of the research was done between 1992-2000.	2003	12687796
sin nombre virus glycoprotein trafficking.	sin nombre virus (snv) is a major representative of the new world hantaviruses and the most common cause of hantavirus pulmonary syndrome (hps) with high mortality in north america. unlike other members of the family bunyaviridae which mature in the golgi complex, new world hantaviruses have been previously reported to mature at the cell surface. for family bunyaviridae viruses, retention of the viral glycoproteins at the golgi complex is thought to be responsible for their golgi maturation. in ...	2003	12706089
human recombinant neutralizing antibodies against hantaan virus g2 protein.	old world hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs), still present a public health problem in asia and eastern europe. the majority of cases has been recorded in china. the aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. to preserve the structural identity of viral protein, the panning procedure was performed on native hantaan (htn) (76-118) virus propagated in vero-e6 cells. in total, five complete ...	2003	12706090
[hantavirus pulmonary syndrome: current situation among rodent reservoirs and human population in the 10th region, chile].	in chile, three hantavirus seropositive rodent species (o longicaudatus, a olivaceus and a longipilis) are distributed from the pacific coast to the andes mountains and represent nearly 90% of the rodents captured in the xth region.	2003	12708255
[hantaviruses].	hantaviruses are zoonotic diseases that affect humans and have a worldwide distribution. the hemorrhagic fever associated with renal syndrome occurs endemically in the asian and european continents affecting housauds of people every year. hantavirus cardiopulmonary syndrome, recognized as a clinical entity since 1993, represents the prototype of emerging diseases and is distributed in countries of the american continent, including brazil. both diseases are transmitted to man through the inhalati ...	2003	12715067
lymphocyte apoptosis during hemorragic fever with renal syndrome.	programmed cell death (pcd) is a fundamental process regulating immune homeostasis. during acute viral infections pcd of lymphocytes by apoptosis is necessary for removing the excess of activated antigen-reactive t cells and down-regulation of the immune response. on the other hand, apoptosis is the key mechanism of elimination of viral-infected cells. at the present time there is virtually no data on mechanisms of immune regulation during human hantavirus infections. in this study we examined t ...	2003	12717553
[hantavirus].		2003	12718024
[hantavirus pulmonary syndrome].		2003	12722234
human tula virus infection or rat-bite fever?		2003	12736795
traveling waves of infection in the hantavirus epidemics.	traveling waves are analyzed in a model of the hantavirus infection in deer mice. the existence of two kinds of wave phenomena is predicted. an environmental parameter governs a transition between two regimes of propagation. in one of them the front of infection lags behind at a constant rate. in the other, fronts of susceptible and infected mice travel at the same speed, separated by a constant delay. the dependence of the delay on system parameters is analyzed numerically and through a piecewi ...	2003	12749537
acute respiratory failure with thrombocytopenia in a 47-year-old woman after hiking in the sierras.		2003	12907570
rapid and simple method for screening wild rodents for antibodies to sin nombre hantavirus.	sin nombre hantavirus (snv) is the primary etiologic agent of hantavirus cardiopulmonary syndrome (hcps) in the united states and canada. hantavirus cardiopulmonary syndrome is a zoonotic disease. the most common reservoir is the deer mouse (peromyscus maniculatus), although numerous other species of wild rodent can carry the viruses that cause hcps throughout the americas. infected rodents show no signs of clinical disease but they develop persistent infection. sin nombre virus can be contracte ...	2003	12910753
analyses of gene flow among populations of deer mice (peromyscus maniculatus) at sites near hantavirus pulmonary syndrome case-patient residences.	gene flow and potential for sin nombre virus (snv) trafficking of the deer mouse (peromyscus maniculatus) was studied in delta and mesa counties of western colorado (usa). the study areas included grand mesa and surrounding grazing and agricultural areas. this area has several natural potential barriers to rodent gene flow, including rivers, cliffs, and mountains. ten study sites were utilized in a spatially nested design ranging from 0.65-81 km apart; four of these sites were at or near human h ...	2003	12910755
hantavirus antibody occurrence in bank voles (clethrionomys glareolus) during a vole population cycle.	puumala virus, genus hantavirus, is the etiologic agent of nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. the bank vole (clethrionomys glareolus) is the natural reservoir species of this hantavirus. we initiated sampling of bank voles at sites of recently identified human nephropathia epidemica cases and paired control sites in the fall of 1995 in coastal areas of northern sweden. sites were trapped annually in spring and fall until 1999. prevalence of antibody to ...	2003	12910756
active and passive vaccination against hantavirus pulmonary syndrome with andes virus m genome segment-based dna vaccine.	hantavirus pulmonary syndrome (hps) is a rapidly progressing human disease with one of the highest case fatality rates (30 to 50%) of any acute viral disease known. there are no vaccines, effective antiviral drugs, or immunologics to prevent or treat hps. in an attempt to develop hps medical countermeasures, we constructed an expression plasmid, pwrg/and-m, that contains the full-length m genome segment of andes virus (andv), a south american hantavirus. transfection experiments in cell culture ...	2003	12941899
serological survey of hantavirus in jardinopolis county, brazil.	hantaviruses are the causative agents of severe human diseases such as cardiopulmonary syndrome (cps), described for the first time in 1993. approximately 200 cases of this emergent disease were reported in brazil until august 2002, with a 40% fatality rate. in may 2001, a hantavirus serologic survey was carried out among 15-70-year-old inhabitants of jardinopolis county, state of são paulo, brazil. blood samples of 818 inhabitants from urban and rural areas were collected by digitopuncture and ...	2003	12966548
hemorrhagic fever with renal syndrome presenting with intrauterine fetal death. a case report.	hantavirus infection in pregnancy is rare. only 2 cases of hantavirus pulmonary syndrome have been reported in the english-language literature. we report a case of hemorrhagic fever with renal syndrome (hfrs) complicating pregnancy to alert clinicians to this rare possibility.	2003	12971153
synthesis of seoul virus rna and structural proteins in cultured cells.	seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). the virion has a tripartite (s, m, and l) negative-stranded rna genome, which is characteristic of the family bunyaviridae. however, the molecular basis of virus replication is not well known. we established a northern blot hybridization (nb) procedure using digoxygenin-labeled rna probes, to quantitate the hantaviral plus- and minus-strand rnas separately. virus rna replication was analyzed in infected vero e6 ...	2003	14505081
tyrosine residues direct the ubiquitination and degradation of the ny-1 hantavirus g1 cytoplasmic tail.	the hantavirus g1 protein contains a long c-terminal cytoplasmic tail of 142 residues. hantavirus pulmonary syndrome-associated hantaviruses contain conserved tyrosine residues near the c terminus of g1 which form an immunoreceptor tyrosine activation motif (itam) and interact with src and syk family kinases. during studies of the g1 itam we observed that fusion proteins containing the g1 cytoplasmic tail were poorly expressed. expression of g1 cytoplasmic tail constructs were dramatically enhan ...	2003	14512526
mapping of the regions involved in homotypic interactions of tula hantavirus n protein.	hantavirus nucleocapsid (n) protein has been suggested to form homodimers and homotrimers that are further integrated into the nucleocapsid filaments around the viral rna. here we report detailed mapping of the regions involved in the homotypic n protein interactions in tula hantavirus (tulv). peptide scan screening was used to define the interaction regions, and the mammalian two-hybrid assay was used for the functional analysis of n protein mutants. to study linear regions responsible for n pr ...	2003	14512541
complete sequence of the genome of the human isolate of andes virus chi-7913: comparative sequence and protein structure analysis.	we report here the complete genomic sequence of the chilean human isolate of andes virus chi-7913. the s, m, and l genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall gc content of 38.7%. these genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a rna-dependent rna polymerase of 2,152 amino acids. in addition, the genome also has other orfs coding for putative proteins of 34 ...	2003	14513715
prevalence of antibodies against hantaviruses among the adult population of the czech republic.	at least three hantavirus genospecies are circulating among wild rodents in europe: puumala, dobrava and tula, the two first being of clinical significance for humans as possible causative agents of haemorrhagic fever with renal syndrome. as many as 710 adult individuals randomly selected from 12 regions of the czech republic were screened for the presence of antibodies against hantaviruses. commercial sets with hantaan and puumala antigens were used for this purpose. five subjects showed igg re ...	2003	14514172
hantavirus infection in humans and rodents, northwestern argentina.	we initiated a study to elucidate the ecology and epidemiology of hantavirus infections in northern argentina. the northwestern hantavirus pulmonary syndrome (hps)-endemic area of argentina comprises salta and jujuy provinces. between 1997 and 2000, 30 hps cases were diagnosed in jujuy province (population 512,329). most patients had a mild clinical course, and the death rate (13.3%) was low. we performed a serologic and epidemiologic survey in residents of the area, in conjunction with a serolo ...	2003	14519242
quality control measures for the serological diagnosis of hantavirus infections.	with society's rapidly increasing mobility, patients infected with severe viral infections can become seriously ill at any place in europe and elsewhere. improving the diagnostics of these infections is the most important step in detecting the pathogens and dealing with them, and for this purpose, quality control measures are essential tools.	2003	14522063
hantavirus infections in europe.	hantaviruses are enveloped rna viruses each carried by a specific rodent species. three hantaviruses, puumala, dobrava, and saaremaa viruses, are known to cause haemorrhagic fever with renal syndrome. in europe. puumala causes a generally mild disease, nephropathia epidemica, which presents most commonly with fever, headache, gastrointestinal symptoms, impaired renal function, and blurred vision, whereas dobrava infections often also have haemorrhagic complications. there are few available data ...	2003	14522264
urinary neopterin concentrations in patients with balkan endemic nephropathy (ben).	balkan endemic nephropathy (ben) is of great clinical importance in restricted areas of bulgaria, serbia, croatia, bosnia, herzegovina, and romania, since the etiology of ben is still unknown.	2003	14531816
occurrence of renal and pulmonary syndrome in a region of northeast germany where tula hantavirus circulates.	hantavirus species tula (tulv) is carried by european common voles (microtus spp.). its pathogenic potential for humans is unknown. in a rural region of northeast germany, a 43-year-old man became ill with fever, renal syndrome, and pneumonia. typing of late acute- and convalescent-phase sera by focus reduction neutralization assay revealed the presence of neutralizing antibodies against tulv. moreover, we detected tulv genetic material in microtus arvalis animals that were trapped at places onl ...	2003	14532254
detection of antibody for the serodiagnosis of hantavirus infection in different rodent species.	peroxidase-labeled staphylococcal protein a, streptococcal protein g, and antibodies directed against mus musculus (mouse), rattus norvegicus (rat), mesocretus auratus (hamster), and peromyscus leucopus were examined for their reactivity with immunoglobulin g (igg) from various rodent species. the purpose of this study was to identify the optimal secondary antibodies or reagents for specific serodiagnosis of hantavirus infection in various rodent species. using elisa, a total of 65 sera from 29 ...	2003	14551813
[hantavirus infection as a rodent borne zoonosis].		2003	14556503
generation of a recombinant cytomegalovirus for expression of a hantavirus glycoprotein.	a cytomegalovirus (cmv) was isolated from its natural host, peromyscus maniculatus, and was designated peromyscus cmv (pcmv). a recombinant pcmv was constructed that contained sin nombre virus glycoprotein g1 (snv-g1) fused in frame to the enhanced green fluorescent protein (egfp) gene inserted into a site homologous to the human cmv ul33 (p33) gene. the recombinant cmv was used for expression and immunization of deer mice against snv-g1. the results of the study indicate that p. maniculatus cou ...	2003	14581557
the dynamics of two viral infections in a single host population with applications to hantavirus.	an si epidemic model for a host with two viral infections circulating within the population is developed, analyzed, and numerically simulated. the model is a system of four differential equations which includes a state for susceptible individuals, two states for individuals infected with a single virus, one which is vertically transmitted and the other which is horizontally transmitted, and a fourth state for individuals infected with both viruses. a general growth function with density-dependen ...	2003	14583172
the changing face of infectious disease. new threats, new science.		2003	14593993
prevalence of infection with hantavirus in rodent populations of central argentina.	we studied hantavirus seroprevalence and virus variability in rodent populations in diego gaynor, northwest of buenos aires province, argentina. rodent samplings were conducted in railroads and cropfield borders in march and july 1999, september and december 2000, and march 2001. antibody detection was performed by an enzyme link immunosorbent assay (elisa), using the recombinant nucleoprotein of andes (and) virus as antigen. tissue samples were taken from positive antibody individuals in order ...	2003	14595446
[hantavirus-induced acute renal failure. a case report].	a non-ischemic acute renal failure may indicate a hantavirus nephropathy, so that appropriate serological examinations should be done. a 19-year-old man had acutely fallen ill with fever, rhinitis, and arthralgia. clinically, he presented with a polyuric renal failure as well as hypertension, proteinuria, erythro- and leucocyturia. serologically, igg- and igm-hantavirus antibodies were found against the central european variant of hantavirus dobrava. at renal biopsy, tubular epithelial swelling, ...	2003	14605850
association of the nucleocapsid protein of the seoul and hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules.	we performed yeast two-hybrid screening of a human kidney cell cdna library to study the biological role of the hantavirus nucleocapsid protein (np). we found that seoul virus (seov) and hantaan virus (htnv) nps were associated with small ubiquitin-like modifier (sumo)-1-interacting proteins pias1, piasxbeta, hipk2, chd3, and ttrap, which interacted with the sumo-1 conjugating enzyme (ubc-9) and sumo-1 in the yeast two-hybrid assay. interactions between the hipk2, chd3, and ttrap proteins and se ...	2003	14609633
the complex ecology of hantavirus in paraguay.	following an outbreak of hantavirus pulmonary syndrome (hps) in the paraguayan chaco in 1995, calomys laucha was identified as the rodent host for the hantavirus associated with these cases. to explore the possibility of additional hantaviruses in paraguay, we collected 636 mammals from 10 of the 17 departments. plasma from 27 animals in alto paraguay and boquer6n in the chaco and neembucú and itapúa in the eastern region had antibody to andes virus antigens. of these 27, five individuals (among ...	2003	14628942
genetic similarity of puumala viruses found in finland and western siberia and of the mitochondrial dna of their rodent hosts suggests a common evolutionary origin.	a total of 678 small mammals representing eight species were trapped in western siberia in 1999-2000 and assayed for the presence of hantaviruses. eighteen animals, all clethrionomys species, were antigen positive by enzyme-linked immunosorbent assay (elisa). small and medium genome segments were recovered by rt-pcr from six samples from clethrionomys glareolus and three from clethrionomys rufocanus. sequence comparison and phylogenetic analysis revealed that these hantaviruses were puumala viru ...	2003	14636686
tropical dermatology: viral tropical diseases.	viruses are important pathogens in tropical areas; most of them, especially the tropical hemorrhagic fevers, produce mucocutaneous manifestations. more than any other kind of pathogen, viruses have the possibility for being widespread, since they have a greater probability of mutation than do bacteria, can cross species barriers easily, and infect both human beings and animals in habitats with a great biodiversity. tropical habitats also have been subject to major ecologic changes in the last fe ...	2003	14639375
hantavirus infections in europe.		2003	14652198
[using recombinant antigens of hantavirus to study the kinetics of serum iga, igg, igm antibodies in the acute-phase of hemorrhagic fever renal syndrome].	to observe the features of serum specific iga, igg, igm antibodies in the acute phase of hemorrhagic fever renal syndrome (hfrs).	2003	15340571
[mechanism of viral infection of macrophages by an agent of hemorrhagic fever with renal syndrome: ultrastructural aspects].	monocytes/macrophages are one of the first cells subjected to the infectious effect of viruses. the present paper analyses for the first time the ultrastructural changes in macrophages caused by an agent of hemorrhagic fever with renal syndrome (hers)--hantavirus (hv). after a local fusion with the host cell plasmalemma and its adsorption on the cell surface, the hv penetrates through the macrophage membrane. this process occurred without destruction of cell plasmalemma. hv viral particles were ...	2003	15216629
seoul hantavirus infection mimicking dengue fever.		2003	15190673
[hantavirus cardiopulmonary syndrome: utility of the picco(pulse contour cardiac output) system for monitoring].	we report a 68 years old man, farmer from the metropolitan region, admitted with a hantavirus cardiopulmonary syndrome. the diagnosis was made using serologic test and was later confirmed by the public health institute. he evolved to an early multiple organ failure, requiring high concentrations of oxygen and invasive ventilatory assistance, vasopressor drugs and renal replacement therapy. swan ganz and picco were used simultaneously for hemodynamic monitoring. treatment consisted in global supp ...	2003	14692308
prevalence of rabies virus and hantaan virus infections in commensal rodents and shrews trapped in bangkok.	five hundred rodents and shrews (rattus norvegicus: 458, rattus rattus: 28, rattus exulans: 5, mus musculus: 4 and suncus murine: 5) trapped from the fresh food markets around bangkok area were investigated for rabies virus and hantaan virus infections. no rabies viral antigens in the animals' brains were detected by direct immunofluorescence. on the other hand, antibodies to hantaan virus were demonstrated in the sera of 7 (1.53%) r. norvegicus caught in various markets using a particle aggluti ...	2003	14696782
human hantavirus infections, sweden.	the prevalent human hantavirus disease in sweden is nephropathia epidemica, which is caused by puumala virus and shed by infected bank voles (clethrionomys glareolus). to evaluate temporal and spatial patterns of this disease, we studied 2,468 reported cases from a highly disease-endemic region in northern sweden. we found that, in particular, middle-aged men living in rural dwellings near coastal areas were overrepresented. the case-patients were most often infected in late autumn, when engaged ...	2003	14718081
hantavirus pulmonary syndrome, southern chile.	we analyzed data from 25 consecutive patients with hantavirus pulmonary syndrome (hps) admitted to the puerto montt and osorno regional hospitals, southern chile, from 1997 to 2001, emphasizing epidemiologic, clinical, radiographic, treatment, and laboratory aspects. hemorrhage was frequent (64%), and 48% of patients showed alterations in renal function. ten patients died (40%). we identified three groups of patients, which included the following: 1) those with the least severe form who had prod ...	2003	14718088
non-covalent interaction between nucleocapsid protein of tula hantavirus and small ubiquitin-related modifier-1, sumo-1.	to find cellular binding counterparts for the nucleocapsid protein (n) of tula hantavirus (tulv), two cdna libraries were screened using yeast two-hybrid systems based on lexa and gal4 transcription factors. five cdna clones encoding sumo-1 (small ubiquitin-related modifier, also known as sentrin) were selected in the lexa system. confocal microscopy revealed that, in infected cells, tulv n protein and sumo-1 colocalize at the perinuclear area providing further evidence for interaction between t ...	2003	12606074
hantavirus.	when hantaviruses hit the headlines with the advent in may 1993 of a new disease in the usa, and later in the new world from canada to south argentina, called "hantavirus pulmonary syndrome" (hps), speculations in the lay press rose from the very beginning around the possibilities of a biological warfare (bw) weapon. indeed, the responsible agent of hps, hantavirus, was almost unknown at that moment in the new world, was airborne, seemed to target preferentially young adults, and induced a devas ...	2003	12615308
[sero-epidemiological studies of zoonotic infections in hunters--comparative analysis with veterinarians, farmers, and abattoir workers].	the aim of this study was to investigate seroprevalences to zoonotic pathogens in hunters, to compare the results with other predisposed occupational groups already investigated and to propose preventive measures. blood samples were taken from 146 male and 3 female hunters from the provinces of styria and burgenland in the south-east of austria and anamnestic data were obtained using a questionnaire. the serological investigations included the following bacterial, viral and parasitic zoonotic ag ...	2003	15508783
generation of an hfrs patient-derived neutralizing recombinant antibody to hantaan virus g1 protein and definition of the neutralizing domain.	hantaan virus (htnv) in the hantavirus genus, family bunyaviridae, is the major cause of severe hemorrhagic fever with renal syndrome (hfrs). we prepared a combinatorial phage display library of human fabs to htnv from rna extracted from the blood lymphocytes of a convalescent hfrs patient. we selected two g1 glycoprotein-specific clones and one nucleocapsid protein (n)-specific clone from the fab library for further studies. the human fab antibodies were converted to igg form in baculovirus/ins ...	2003	12436484
ribavirin causes error catastrophe during hantaan virus replication.	except for ribavirin, no other antiviral drugs for treating hantaviral diseases have been identified. it is well established that ribavirin will inhibit the production of infectious hantaan virus (htnv); however, its mechanism of action is unknown. to characterize the inhibitory effect of ribavirin on htnv, the levels of viral rnas, proteins, and infectious particles were measured for 3 days posttreatment of htnv-infected vero e6 cells. htnv-infected cells treated with ribavirin showed a slight ...	2003	12477853
genetic interaction between distinct dobrava hantavirus subtypes in apodemus agrarius and a. flavicollis in nature.	dobrava virus (dobv) occurs in two different rodent species, apodemus flavicollis (dobv-af) and a. agrarius (dobv-aa). we sequenced the s and m genomic segments from sympatric dobv-af and dobv-aa strains which fell into two distinct genetic lineages. molecular phylogenetic analyses gave evidence for genetic reassortment between s and m segments of dobv-af and dobv-aa and indicated homologous recombination events in dobv evolution. dobv-af and dobv-aa are distinct but also subject to genetic exch ...	2003	12477889
the multimerization of hantavirus nucleocapsid protein depends on type-specific epitopes.	multimerization of the hantaan virus nucleocapsid protein (np) in hantaan virus-infected vero e6 cells was observed in a competitive enzyme-linked immunosorbent assay (elisa). recombinant and truncated nps of hantaan, seoul, and dobrava viruses lacking the n-terminal 49 amino acids were also detected as multimers. although truncated nps of hantaan virus lacking the n-terminal 154 amino acids existed as a monomer, those of seoul and dobrava formed multimers. the multimerized truncated np antigens ...	2003	12502810
persistent sin nombre virus infection in the deer mouse (peromyscus maniculatus) model: sites of replication and strand-specific expression.	to address sin nombre (sn) virus persistence in deer mice, we sacrificed experimentally infected deer mice at eight time points from day 21 to day 217 postinoculation (p.i.) and examined their tissues for viral nucleocapsid (n) antigen expression and both negative-strand (genomic) and positive-strand (replicative/mrna) viral s segment rna titers. all the animals that we inoculated developed persistent infections, and sn virus could be isolated from tissues throughout the course of infection. the ...	2003	12502867
hantavirus pulmonary syndrome-associated hantaviruses contain conserved and functional itam signaling elements.	hantaviruses infect human endothelial and immune cells, causing two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we have identified key signaling elements termed immunoreceptor tyrosine-based activation motifs (itams) within the g1 cytoplasmic tail of all hps-causing hantaviruses. itams direct receptor signaling within immune and endothelial cells and the presence of itams in all hps-causing hantaviruses provides a means for altering norma ...	2003	12502882
chilean research throws light on hantavirus transmission.		2003	12505021
hantaviruses. a short review.	hantaviruses are rodent viruses that have been identified as etiologic agents of 2 diseases of humans: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). this article presents a concise review of hantavirus biology, the medical features of hfrs and hps, and tests for the detection of hantavirus infections in humans.	2003	12521363
use of vesicular stomatitis virus pseudotypes bearing hantaan or seoul virus envelope proteins in a rapid and safe neutralization test.	a vesicular stomatitis virus (vsv) pseudotype bearing hantavirus envelope glycoproteins was produced and used in a neutralization test as a substitute for native hantavirus. the recombinant vsv, in which the enveloped protein gene (g) was replaced by the green fluorescent protein gene and complemented with g protein expressed in trans (vsvdeltag*g), was kindly provided by m. a. whitt. 293t cells were transfected with plasmids for the expression of envelope glycoproteins (g1 and g2) of htnv or se ...	2003	12522053
genetic characterization of new dobrava hantavirus isolate from greece.	the first complete genome sequence of dobrava hantavirus isolated from yellow-necked mouse apodemus flavicollis trapped in the northeastern greece is described. the s, m, and l segments of the greek isolate of dobrava virus are 1673, 3635, and 6532 nucleotides (nt) long, respectively, and encode the nucleocapsid (n) protein of 429 amino acids (aa), glycoprotein precursor of 1135 aa, and the l protein of 2151 aa. n protein contains three cysteine residues conserved in all known hantaviruses, as w ...	2003	12526053
examining unmet needs in infectious disease.	in the past 30 years, more than 30 new aetiological agents of infectious disease have been identified. some of these are responsible for entirely novel and life-threatening disorders, such as aids, ebola fever, hantavirus pulmonary syndrome and nipah virus encephalitis. during the same period, some longstanding infectious diseases (such as tuberculosis) have became resurgent, as a result of a combination of complacency, increased travel and social dislocation, and also increasing drug resistance ...	2003	12546988
hantaviruses: etiologic agents of rare, but potentially life-threatening zoonotic diseases.		2003	12555978
diagnostic rapid tests for acute hantavirus infections: specific tests for hantaan, dobrava and puumala viruses versus a hantavirus combination test.	hantaviruses infecting humans in eurasia include hantaan, seoul, puumala and the closely related dobrava and saaremaa viruses. these viruses are causative agents of hemorrhagic fever with renal syndrome (hfrs), which is recognized as a severe health care problem in several countries. diagnostics of hantavirus infections relies on serology, performed principally with enzyme immunoassay (eia) or immunofluorescence assay (ifa). we developed four 5-min immunochromatographic igm-antibody tests for di ...	2003	12565162
hantavirus.	sin nombre virus (snv), an emerging pathogen in the united states, was identified in 1993. this hantavirus, a member of the bunyaviridae family of rna viruses, is transmitted by its reservoir host peromyscus maniculatus, the deer mouse. transmission is by inhalation of aerosolized feces, urine, or saliva from the infected mice. the illness that pursues, hantavirus pulmonary syndrome (hps), is characterized initially by mild flu-like symptoms, followed by rapid progression to respiratory distress ...	2003	12587657
patterns of puumala virus infection in finland.	puumala hantavirus infection is prevalent throughout most of europe, and in endemic areas it may be the most common cause of acute renal failure. to evaluate trends in incidence of puumala virus infections in finland, we analysed national surveillance data in 12-month periods from march 1995 to february 2002. during this time, 8184 laboratory-confirmed cases were notified to the national infectious disease register. three epidemic periods were identified, for which the number of cases was more t ...	2003	12631978
the potential role of viral pathogens as agents of bioterrorism.		2003	12632851
establishment of an enzyme-linked immunosorbent assay for detection of hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in escherichia coli.	a recombinant nucleocapsid protein of hantaan virus (htn) 76-118 strain expressed in e. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rhtn-elisa) for detection of hantavirus antibody in rat sera. the sensitivity and specificity of the rhtn-elisa were compared with those of the indirect immunofluoresent assay (ifa) using virus-infected cells. the sensitivity of rhtn-elisa was similar to that of the ifa both in experimentally sr-11 infected rat and naturally ...	2003	12638233
rescue of hantaan virus minigenomes.	hantavirus infections are a major public health concern worldwide. their widespread geographical distribution and their ability to produce serious, often fatal, human disease underline the need for a system that allows manipulation of these viruses. we describe here the first successful establishment of a reverse genetics technology for hantaan virus, the prototype of the genus hantavirus. the system offers a unique opportunity to study the biology of hantaviruses, the pathogenesis of the diseas ...	2003	12642095
removing deer mice from buildings and the risk for human exposure to sin nombre virus.	trapping and removing deer mice from ranch buildings resulted in an increased number of mice, including sin nombre virus antibody-positive mice, entering ranch buildings. mouse removal without mouse proofing will not reduce and may even increase human exposure to sin nombre hantavirus.	2003	12643840
[detection of hantavirus antigen in rodents in the czech republic].	the work is based on the hitherto implemented examinations of lungs of rodents from the territory of the czech republic for the presence of hantavirus antigen. for detection of a species specific antigen the elisa set hantagnost from the poliomyelitis institute in moscow was used. a total of 1494 micromammalia of 15 species caught in 1991-2001 were examined. the hantavirus antigen was detected in the lungs of 101 individuals (6.8%) whereby representatives of the species microtus arvalis predomin ...	2003	12647557
genetic interaction between dobrava and saaremaa hantaviruses: now or millions of years ago?		2003	12768038
hantaviruses in the czech republic.		2003	12781027
design of oligonucleotide arrays to detect point mutations: molecular typing of antibiotic resistant strains of neisseria gonorrhoeae and hantavirus infected deer mice.	microarrays are promising tools for use in molecular diagnostics due to their ability to perform a multitude of tests simultaneously. in the case of genotyping many such tests will require discrimination of sequence at the single nucleotide level. a number of challenges exist including binding of optimal quantities of probe to the chip surface, the use of uniform hybridization conditions across the chip and the generation of labeled target. we investigated two model systems to test out the effic ...	2003	12788028
quantitation of antibody-bound and unbound sin nombre virus in the plasma of patient with hantavirus pulmonary syndrome.	hantavirus pulmonary syndrome (hps) is a rare but acute fulminant disease caused by sin nombre virus and other related hantaviruses. we reported earlier that hps patients had high levels of viremia and the levels of viremia correlated with the severity of the disease. to determine whether the viruses are free in plasma or bound to antibodies to form immune complexes, we established a method to quantitate viral genomic rna in antibody-bound viruses and in unbound viruses separately. to isolate im ...	2003	12798243
hantavirus infection in people inhabiting a highly endemic region of the gran chaco territory, paraguay: association with trypanosoma cruzi infection, epidemiological features and haematological characteristics.	the seroprevalences of anti-hantavirus antibodies were determined in 712 individuals (551 indians, 140 mennonites of german ancestry, and 21 paraguayans of spanish ancestry) inhabiting a region of western paraguay in the gran chaco territory of south america. the overall seroprevalence of hantavirus infection among the 712 subjects, who were aged 2-80 years, was 42.7% (45.2% in the indians and 34.2% in the non-indians). of the 672 subjects also checked for antibodies against trypanosoma cruzi, 2 ...	2003	12803858
evidence of the presence of seoul virus in cambodia.	a study was conducted in agricultural and urban areas in cambodia to assess the presence of hantaviruses in rodent populations. in 1998, rodents were trapped in two villages and in phnom penh city around market places and a rubbish dump. igg antibodies to hantaan virus were detected in 54 (8.2%) rodents among 660 tested: 6.4% (13/203) among roof rats (rattus rattus), 20.9% (39/187) among norway rats (r. norvegicus), 16.7% (2/12) among unidentified rattus species and none in 183 polynesian rats ( ...	2003	12850202
expression of the nucleoprotein of the puumala virus from the recombinant semliki forest virus replicon: characterization and use as a potential diagnostic tool.	puumala virus (bunyaviridae family, hantavirus genus) causes a mild form of hemorrhagic fever with renal syndrome (hfrs) called nephropathia epidemica in northern and central europe. serological tests are used for diagnosis, but antigen production is difficult because the virus grows poorly in tissue culture. we expressed the n protein (nucleoprotein) of puumala virus via the semliki forest virus (sfv) replicon in mammalian cells and compared its antigenic properties with those of the native ant ...	2003	12853401
hantavirus-specific cd8(+)-t-cell responses in newborn mice persistently infected with hantaan virus.	the relationship between virus-specific cd8(+)-t-cell responses and viral persistence was studied in mice by using hantaan virus (htnv). we first established a simple method for measuring levels of virus-specific cd8(+) t cells by flow cytometry. next, to produce a mouse model of persistent htnv infection, newborn mice were inoculated subcutaneously within 24 h of birth with 1 or 0.1 50% newborn mouse lethal dose of htnv. all mice that escaped lethal infection were persistently infected with htn ...	2003	12857910
genetic characterisation of a hantavirus isolated from a laboratory-acquired infection.	seoul (seo) viruses belong to the hantavirus genus (family bunyaviridae), cause the moderate form of haemorrhagic fever with renal syndrome, and have been associated with laboratory-acquired infections in many countries. to investigate the pedigree of an isolate, designated ir461, which was obtained from a laboratory-acquired infection in a uk research institute, we determined the nucleotide sequences of the small (s) and medium (m) genome segments. in addition, we determined the sequences of th ...	2003	12858415
hantaviruses induce cytopathic effects and apoptosis in continuous human embryonic kidney cells.	hantaviruses are maintained in nature in persistently infected rodents and can also persistently infect cultured mammalian cells, causing little or no cytopathology. an unexpected outcome of this study was the observation of cytopathic effects (cpe) in the hantavirus-infected human embryonic kidney cell line hek293. it was confirmed that hantaviruses induce apoptosis in hek293 cells, although apoptosis appeared mostly in uninfected, bystander cells and rarely in infected hek293 cells. although s ...	2003	12867652
[molecular characterization of hantavirus shandong isolate jnl virus strain].	to understand the molecular epidemiologic characteristics of hantavirus shandong isolate jnl virus strain.	2003	12869988
[construction and stable expression of intracellular antibodies to glycoprotein of hantavirus].	to understand the molecular mechanisms of hantavirus assembly and maturation by stably expressing the intracellular antibodies to hantavirus glycoprotein g1 and g2 in endoplasmic reticulum (er) and cytoplasm (cyto) of vero e6 cell.	2003	12869989
[molecular analysis of hantavirus isolated from shandong province].	to compare the molecular characteristics of hantaviruses isolated from shandong province by using pcr typing and nucleotide sequencing.	2003	12869990
[serum antibody response and hantavirus rna sequencing in patients with hemorrhagic fever renal syndrome in yantai areas].	to understand antibody responses to and rna sequences of hantavirus in patients with hemorrhagic fever renal syndrome (hfrs) in yantai areas and to demonstrate the type of the prevalent viruses caused hfrs.	2003	12869991
[study of hantavirus seoul in a human and rodent population from a marginal area in buenos aires city].	a sero-epidemiological survey was conducted to detect evidence of the circulation of hantavirus seoul. this virus of worldwide distribution is associated with hemorrhagic fever with renal syndrome. a total of 106 samples from people who live in a marginal area in buenos aires city and 29 rattus norvegicus captured in the surroundings of their houses were tested for specific antibodies. thirty five samples from hypertensive patients undergoing a follow up health program, living in the same neighb ...	2003	12876901
hantavirus infections in spain: analysis of sera from the general population and from patients with pneumonia, renal disease and hepatitis.	hantaviruses are rodent borne viruses in the family bunyaviridae that cause significant morbidity in large areas of europe. there are only a few reports available on hantavirus infections from spain. although the results of these earlier studies indicated the presence of hantavirus infections, no confirmative or serotype-specific analyses have been performed.	2003	12878094
adaptation of puumala hantavirus to cell culture is associated with point mutations in the coding region of the l segment and in the noncoding regions of the s segment.	we previously developed a model for studies on hantavirus host adaptation and initiated genetic analysis of puumala virus variants passaged in colonized bank voles and in cultured vero e6 cells. with the data presented in this paper, the sequence comparison of the wild-type and vero e6-adapted variants of puumala virus, strain kazan, has been completed. the only amino acid substitution that distinguished the two virus variants was found in the l protein, ser versus phe at position 2053. another ...	2003	12885898
hantavirus prevalence in the ix region of chile.	an epidemiologic and seroprevalence survey was conducted (n=830) to assess the proportion of persons exposed to hantavirus in ix region chile, which accounts for 25% of reported cases of hantavirus cardiopulmonary syndrome. this region has three geographic areas with different disease incidences and a high proportion of aboriginals. serum samples were tested for immunoglobulin (ig) g antibodies by enzyme-linked immunosorbent assay against sin nombre virus n antigen by strip immunoblot assay agai ...	2003	12890323
yellow pigmy rice rat (oligoryzomys flavescens) and hantavirus pulmonary syndrome in uruguay.	during 5,230 trapping nights, 672 small mammals were trapped in the areas where most hantavirus pulmonary syndrome (hps) cases occur in uruguay. yellow pygmy rice rats (oligoryzomys flavescens) were the only rodents that showed evidence of antibodies to hantavirus, with a seroprevalence of 2.6%. the rodents were trapped in all the explored environments, and most of the seropositive rodents were found in habitats frequented by humans. nucleotide sequences were obtained from four hps case-patients ...	2003	12890326