Publications

complement activation in puumala hantavirus infection correlates with disease severity.	abstract introduction. hantaviruses are important human pathogens that cause clinical diseases characterized by renal and cardiopulmonary manifestations. their pathogenesis is currently poorly understood. we have studied the role of the complement system in the pathogenesis of puumala (puuv) hantavirus infection. material and methods. we studied the activation of complement by measuring the terminal complement complex sc5b-9 and complement component c3 and c4 levels in patients with acute puuv i ...	2011	21495786
a new subtype of thottapalayam virus carried by the asian house shrew (suncus murinus) in china.	recently, many novel hantavirus species carried by insectivore had been found. in order to know whether the insectivore-associated hantaviruses exist in china, asian house shrew (suncus murinus) were analyzed for the presence of hantaviruses. 362 asian house shrews were captured in wenzhou of zhejiang province. hantavirus-specific rna was detected by rt-pcr in lung tissue samples of six shrews. the complete hantavirus s and m segment sequences and also partial l segment sequences were successful ...	2011	21801855
viral infections in workers in hospital and research laboratory settings: a comparative review of infection modes and respective biosafety aspects.	to compare modes and sources of infection and clinical and biosafety aspects of accidental viral infections in hospital workers and research laboratory staff reported in scientific articles.	2011	21497126
structural characterization of the crimean-congo hemorrhagic fever virus gn tail provides insight into virus assembly.	the rna virus that causes the crimean congo hemorrhagic fever (cchf) is a tick-borne pathogen of the nairovirus genus, family bunyaviridae. unlike many zoonotic viruses that are only passed between animals and humans, the cchf virus can also be transmitted from human to human with an overall mortality rate approaching 30%. currently, there are no atomic structures for any cchf virus proteins nor for any nairovirus proteins. a critical component of the virus is the envelope gn glycoprotein, which ...	2011	21507948
biogeographic and ecological regulation of disease: prevalence of sin nombre virus in island mice is related to island area, precipitation, and predator richness.	the relative roles of top-down and bottom-up forces in affecting disease prevalence in wild hosts is important for understanding disease dynamics and human disease risk. we found that the prevalence of sin nombre virus (snv), the agent of a severe disease in humans (hantavirus pulmonary syndrome), in island deer mice from the eight california channel islands was greater with increased precipitation (a measure of productivity), greater island area, and fewer species of rodent predators. in findin ...	2011	21508614
human pathogenic hantaviruses and prevention of infection.	hantaviruses are emerging viruses which are hosted by small mammals. when transmitted to humans, they can cause two clinical syndromes, hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome. the review compiles the current list of hantaviruses which are thought to be pathogenic in humans on the basis of molecular or at least serological evidence. whereas induction of a neutralizing humoral immune response is considered to be protective against infection, the dual role of c ...	2011	21508676
platelet ligands and adamts13 during puumala hantavirus infection and associated thrombocytopenia.	we aimed here to elucidate the role of adhesive platelet ligands and endothelial involvement during the acute phase of puumala hantavirus (puuv) infection. nineteen hospital-treated patients with serologically confirmed diagnosis of acute puuv infection were included. patient charts were reviewed for clinical and basic laboratory data. plasma levels of von willebrand factor antigen (vwf: ag), ristocetin cofactor (vwf: rco), factor viii (fviii: c) and a disintegrin and metalloproteinase with a th ...	2011	21508829
presence of activated airway t lymphocytes in human puumala hantavirus disease.	hantaviruses cause two clinical syndromes: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the clinical spectrum in hfrs also often involves respiratory symptoms. as information about the pulmonary pathogenesis in hfrs is limited, we aimed to further study the local airway immune response in the lower airways.	2011	21436245
the delicate pygmy rice rat (oligoryzomys delicatus) is the principal host of maporal virus (family bunyaviridae, genus hantavirus).	abstract choclo virus (chov) and maporal virus (mapv) are enzootic in panama and western venezuela, respectively. the results of previous studies suggested that the fulvous pygmy rice rat (oligoryzomys fulvescens) is the principal host of both viruses. the results of an analysis of nucleotide sequence data in this study indicated that the rodent associated with chov is the costa rican pygmy rice rat (oligoryzomys costaricensis) and that the rodent associated with mapv is the delicate pygmy rice ...	2011	21548760
maporal virus as a surrogate for pathogenic new world hantaviruses and its inhibition by favipiravir.	pathogenic hantaviruses geographically distributed in the old world cause haemorrhagic fever with renal syndrome (hfrs), whereas new world hantaviruses are the aetiological agents of hantavirus cardiopulmonary syndrome (hcps). ribavirin, a drug associated with toxicities, is presently indicated for treatment of hfrs, whereas treatment of the more frequently lethal hcps is limited to supportive care. because of the need for safe and effective antivirals to treat severe hantaviral infections, we e ...	2011	21566265
detection of hantaviruses in brazilian rodents by sybr-green-based real-time rt-pcr.	current knowledge of the pathogenic hantavirus indicates that wild rodents are its primary natural reservoir. specific primers to detect the presence of viral genomes were developed using an sybr-green-based real-time rt-pcr protocol. one hundred sixty-four rodents native to the atlantic forest biome were captured in são paulo state, brazil, and their tissues were tested. the presence of hantavirus rna was detected in sixteen rodents: three specimens of akodon montensis, three of akodon cursor, ...	2011	21442233
expression of recombinant araraquara hantavirus nucleoprotein in insect cells and its use as an antigen for immunodetection compared to the same antigen expressed in escherichia coli.	abstract: background: antigens for hantavirus serological tests have been produced using dna recombinant technology for more than twenty years. several different strategies have been used for that purpose. all of them avoid the risks and difficulties involved in multiplying hantavirus in the laboratory. in brazil, the araraquara virus is one of the main causes of hantavirus cardio-pulmonary syndrome (hcps). methods. in this investigation, we report the expression of the n protein of the araraqua ...	2011	21569341
glomerulonephritis emerging shortly after puumala hantavirus infection: a report on 7 patients.	aims: nephropathia epidemica (ne) is mild type of hemorrhagic fever caused by puumala (puu) hantavirus. renal biopsy typically shows acute tubulointerstitial nephritis and complete recovery is the usual outcome. we previously described 5 patients with membranoproliferative glomerulonephritis (mpgn) after acute ne. we now report on 7 more patients who developed biopsy-confirmed glomerulonephritis (gn) during the convalescent phase of ne. material and methods: we present case histories of 7 patien ...	2011	21612760
detection of dobrava-belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and sybr green-based real-time reverse transcriptase-polymerase chain reaction.	dobrava (dobv) hantaviruses belong to the genus hantavirus, family bunyaviridae, and are carried by yellow-necked and striped field mice. the goal of this study was to detect dobv using serological and genetic methods in apodemus rodents in hungary and in northern croatia. during the study period, a total of 125 apodemus sp. (67 a. agrarius, 58 a. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rrt-pcr and/or elisa. we conclude that the prevalence ...	2011	21573689
phylogenetic analysis of puumala virus subtype bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein.	puumala virus (puuv) is the predominant hantavirus species in germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (hfrs). during an outbreak in south-east germany in 2004 a novel puuv subtype designated bavaria was identified as the causative agent of hfrs in humans [1]. here we present a molecular characterization of this puuv strain by investigating novel partial and almost entire nucleocapsid (n) protein-encoding small (s-) segment sequences and p ...	2011	21598005
recent advances in hantavirus molecular biology and disease.	hantaviruses are emerging zoonotic pathogens that belong to the bunyaviridae family. they have been classified as category a pathogens by cdc (centers for disease control and prevention). hantaviruses pose a serious threat to human health because their infection causes two highly fatal diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps). these pathogens are transmitted to humans through aerosolized excreta of their infected rodent hosts. hantavir ...	2011	21459193
genetic diversity of imjin virus in the ussuri white-toothed shrew (crocidura lasiura) in the republic of korea, 2004-2010.	recently, imjin virus (mjnv), a genetically distinct hantavirus, was isolated from lung tissues of the ussuri white-toothed shrew (crocidura lasiura) captured near the demilitarized zone in the republic of korea. to clarify the genetic diversity of mjnv, partial m- and l-segment sequences were amplified from lung tissues of 12 of 37 (32.4%) anti-mjnv igg antibody-positive ussuri white-toothed shrews captured between 2004 and 2010. a 531-nucleotide region of the m segment (coordinates 2,255 to 2, ...	2011	21303516
procalcitonin in hantavirus infections.	in hantavirus infections levels of serum leukocytes or c-reactive protein are usually elevated to levels found in serious bacterial infections. however, procalcitonin in patients infected with hantavirus has not yet been discussed in the literature. a total of 29 adult patients with hantavirus infection, 30 with sepsis, and 19 with tick-borne encephalitis were included in this observational retrospective study. the median procalcitonin level in patients with hantavirus infection was 0.53 µg/l (r ...	2011	21344982
hantavirus protein interactions regulate cellular functions and signaling responses.	rodent-borne pathogenic hantaviruses cause two severe and often lethal zoonotic diseases: hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus cardiopulmonary syndrome (hcps) in the americas. currently, no us fda-approved therapeutics or vaccines are available for hfrs/hcps. infections with hantaviruses are not lytic, and it is currently not known exactly why infections in humans cause disease. a better understanding of how hantaviruses interfere with normal cell functions and ...	2011	21171876
using geographic information system-based ecologic niche models to forecast the risk of hantavirus infection in shandong province, china.	hemorrhagic fever with renal syndrome (hfrs) is an important public health problem in shandong province, china. in this study, we combined ecologic niche modeling with geographic information systems (gis) and remote sensing techniques to identify the risk factors and affected areas of hantavirus infections in rodent hosts. land cover and elevation were found to be closely associated with the presence of hantavirus-infected rodent hosts. the averaged area under the receiver operating characterist ...	2011	21363991
genetic evidence of hantavirus infections in wild rodents from northwestern colombia.	this report builds on recent serological evidence for the presence of hantavirus in northern colombia by providing sequence-specific and phylogenetic data of hantavirus infections in wild rodents. from august 2007 to august 2008, 354 rodent specimens representing four families were collected in the northwestern antioquia region of colombia. antibodies reactive to sin nombre virus and maciel virus antigens by igg enzyme-linked immunosorbent assay were found in 15 of 109 (14%) cherries cane rats ( ...	2011	21284514
time to revise the paradigm of hantavirus syndromes? hantavirus pulmonary syndrome caused by european hantavirus.	hantaviruses have previously been recognised to cause two separate syndromes: hemorrhagic fever with renal syndrome in eurasia, and hantavirus pulmonary syndrome (hps) in the americas. however, increasing evidence suggests that this dichotomy is no longer fruitful when recognising human hantavirus disease and understanding the pathogenesis. herein are presented three cases of severe european puumala hantavirus infection that meet the hps case definition. the clinical and pathological findings we ...	2011	21234633
characterization of the interaction between hantavirus nucleocapsid protein (n) and ribosomal protein s19 (rps19).	hantaviruses, members of the bunyaviridae family, are negative-stranded emerging rna viruses and category a pathogens that cause serious illness when transmitted to humans through aerosolized excreta of infected rodent hosts. hantaviruses have evolved a novel translation initiation mechanism, operated by nucleocapsid protein (n), which preferentially facilitates the translation of viral mrnas. n binds to the ribosomal protein s19 (rps19), a structural component of the 40 s ribosomal subunit. in ...	2011	21296889
hantavirus pulmonary syndrome: encephalitis caused by virus andes.	hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome (hps) are rodent-borne emerging diseases caused by members of the genus hantavirus, family bunyaviridae. some species of hantavirus may cause encephalitis, but this is the first report in andes virus associated to hps.	2011	21240581
interview with pedro fernando da costa vasconcelos, md, phd. interview by vicki glaser.		2011	21261512
inactivation of hantaviruses by n-ethylmaleimide preserves virion integrity.	thiol groups of cysteine residues are crucial for the infectivity of various enveloped viruses, but their role in the infectivity of viruses of the family bunyaviridae has thus far not been studied. this report shows that thiol groups are essential to the infectivity of hantaviruses. alkylation of the thiol functional groups using the membrane-permeable compound n-ethylmaleimide (nem) and membrane-impermeable compound 5,5'-dithio-bis-(2-nitrobenzoic acid) (dtnb) showed nem to be a highly effecti ...	2011	21289161
high activity of indoleamine 2,3-dioxygenase is associated with renal insufficiency in puumala hantavirus induced nephropathia epidemica.	nephropathia epidemica (ne) is a hemorrhagic fever with renal syndrome caused by puumala hantavirus. the severity of ne varies greatly. the aim of the present study was to evaluate whether serum indoleamine 2,3-dioxygenase (ido) activity is associated with the severity of ne. a prospectively collected cohort of 102 consecutive patients with acute serologically confirmed ne was examined. serum kynurenine, tryptophan, creatinine, crp, and blood cell count were measured for up to 5 consecutive days ...	2011	21328391
rodent community structure and andes virus infection in sylvan and peridomestic habitats in northwestern patagonia, argentina.	modifications of natural habitat in peridomestic rural areas could affect original rodent community composition, diversity, and evenness. in zoonoses such as hantavirus pulmonary syndrome, the presence of a diverse community can dilute the impact of the principal reservoir, reducing risk to humans. the goal of this study was to examine rodent community composition, abundance of andes virus (andv) host (oligoryzomys longicaudatus), andv prevalence, and temporal variability associated with rural p ...	2011	21332352
development and application of a flow cytometric potency assay for dna vaccines.	we have developed a rapid, reliable, and sensitive quantitative flow cytometric assay to measure the in vitro potency and stability of dna vaccines to be delivered either by particle-mediated epidermal delivery (pmed) or by electroporation. the method involves transfecting cells with test dna and comparing the measured antigen expression to that generated with expression from known quantities of reference material dna. the assay was adapted for performance under good laboratory practice (glp) gu ...	2011	21219978
specific humoral reaction of hemorrhagic fever with renal syndrome (hfrs) patients in china to recombinant nucleocapsid proteins from european hantaviruses.	hemorrhagic fever with renal syndrome (hfrs) is endemic in east asia and europe. this study was initiated to investigate the reactivity of antibodies in sera of chinese hfrs patients with the recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), dobrava-belgrade virus (dobv), and puumala virus (puuv), which are the prevalent hantavirus strains in europe. forty-eight pairs of acute and convalescent sera were collected from hfrs patients in hubei, china (1985-2002) and tested by indire ...	2011	21222012
hantavirus infection in istanbul, turkey.		2011	21291612
genetic detection of dobrava/belgrade virus, bulgaria.		2011	21291615
arenaviruses and hantaviruses: from epidemiology and genomics to antivirals.	the arenaviruses and hantaviruses are segmented genome rna viruses that are hosted by rodents. due to their association with rodents, they are globally widespread and can infect humans via direct or indirect routes of transmission, causing considerable human morbidity and mortality. nevertheless, despite their obvious and emerging importance as pathogens, there are currently no effective antiviral drugs (except ribavirin which proved effective against lassa virus) with which to treat humans infe ...	2011	21356244
concomitant influence of helminth infection and landscape on the distribution of puumala hantavirus in its reservoir, myodes glareolus.	puumala virus, the agent of nephropathia epidemica (ne), is the most prevalent hantavirus in europe. the risk for human infection seems to be strongly correlated with the prevalence of puumala virus (puuv) in populations of its reservoir host species, the bank vole myodes glareolus. in humans, the infection risks of major viral diseases are affected by the presence of helminth infections. we therefore proposed to analyse the influence of both helminth community and landscape on the prevalence of ...	2011	21303497
infection of human monocyte-derived dendritic cells by andes hantavirus enhances pro-inflammatory state, the secretion of active mmp-9 and indirectly enhances endothelial permeability.	abstract:	2011	21569520
the c-terminal 42 residues of the tula virus gn protein regulate interferon induction.	hantaviruses primarily infect the endothelial cell lining of capillaries and cause two vascular permeability-based diseases. the ability of pathogenic hantaviruses to regulate the early induction of interferon determines whether hantaviruses replicate in endothelial cells. tula virus (tulv) and prospect hill virus (phv) are hantaviruses which infect human endothelial cells but fail to cause human disease. phv is unable to inhibit early interferon (ifn) responses and fails to replicate within hum ...	2011	21367904
[bilateral acute angle-closure glaucoma due to an infection with hantavirus].	a poor general condition of patients with hemorrhagic fever and elevated creatinine should raise suspicion of a systemic infection with hantavirus. this can often also cause ocular changes. above all, changes of intraocular pressure, in our case bilateral acute angle-closure glaucoma, due to edema and hemorrhage in the ciliary body result in anterolateral rotation of the iris-lens diaphragm with closure of the chamber angle. it can also cause a transient myopia, and intraretinal hemorrhages can ...	2011	21327947
sero-epidemiological study of the presence of hantaviruses in domestic dogs and cats from belgium.	hantaviruses are worldwide rodent-borne pathogens infecting humans and other animals mainly through inhalation of aerosols contaminated with rodent excreta. few data are available on hantavirus serology and geographical distribution in dogs and cats. we therefore screened sera from pet dogs (n=410) and cats (n=124) in two regions of belgium, using igg elisa and ifa. we analysed the effect of the owner's address as well as pet gender and age on hantavirus status. hantavirus antibodies were found ...	2011	21388647
seroepidemiological survey of hantavirus infection in healthy people in vallès occidental, barcelona.	hantaviruses are the etiological agents of hemorrhagic fever with renal syndrome in europe and asia, and hantavirus pulmonary syndrome in america. approximately 150,000 cases are reported annually worldwide. in spain, some hantavirus infection cases have been described. besides, rodents that have been described as hantavirus reservoirs are present. the aim of the present study was to determinate the seroprevalence of hantavirus in humans in the northeast of spain.	2011	21417923
comparison of hantaan and seoul viral infections among patients with hemorrhagic fever with renal syndrome (hfrs) in heilongjiang, china.	hemorrhagic fever with renal syndrome (hfrs) is a serious public health problem in china, and is primarily caused by either the hantaan virus (htnv) or seoul virus (seov) strains. however, the causative hantavirus has only been definitively identified in a few hfrs cases, and detailed comparisons of patient data for the 2 strains are limited.	2011	21428852
hantavirus-specific iga in saliva and viral antigen in the parotid gland in patients with hemorrhagic fever with renal syndrome.	the hantavirus genus comprises rodent borne, zoonotic viruses of the bunyaviridae family that cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus cardiopulmonary syndrome (hcps) in the americas. rodent saliva contains infectious hantavirus and evidence suggests that hantavirus is also shed in human saliva, but person-to-person transmission is rare. in saliva, immunoglobulin (ig) a is the predominant immunoglobulin class. secretory iga serves as an important first line of ...	2011	21360546
hantavirus outbreak in western europe: reservoir host infection dynamics related to human disease patterns.	within europe, puumala virus (puuv) is the causal agent of nephropathia epidemica (ne) in humans, a zoonotic disease with increasing significance in recent years. in a region of belgium with a historically high incidence of ne, bank voles (the puuv reservoir hosts), were monitored for puuv igg antibody prevalence in nine study sites before, during, and after the highest ne outbreak recorded in belgium in 2005. we found that the highest numbers of puuv igg-positive voles coincided with the peak o ...	2011	20450527
a possible case of hantavirus infection in a borneo orangutan and its conservation implication.	natural infection of hantavirus in orangutans has never been reported.	2011	20722771
non-human primates in outdoor enclosures: risk for infection with rodent-borne hantaviruses.	different species of non-human primates have been exploited as animal disease models for human hantavirus infections. to study the potential risk of natural hantavirus infection of non-human primates, we investigated serum samples from non-human primates of three species living in outdoor enclosures of the german primate center (gpc), göttingen, located in a hantavirus endemic region of central germany. for that purpose we used serological assays based on recombinant antigens of the bank vole (m ...	2011	20727685
prospective study on ocular findings in acute puumala hantavirus infection in hospitalised patients.	to appraise the ocular manifestations of puumala hantavirus evoked haemorrhagic fever with renal syndrome nephropathia epidemica (ne) and to clarify the mechanisms of ocular changes in the largest series of patients examined to date.	2011	20679079
puumala virus infection: radiologic findings.	a 33-year-old male patient was admitted to our nephrology department with rapidly deteriorating general health, fever, respiratory difficulties, and acute renal failure. computed tomography of the thorax revealed interstitial edema with thickening of the interlobular septa, peribronchial cuffing, ground-glass opacities, and small pleural and pericardial effusions. polymerase chain reaction tests verified puumala virus infection. the patient recovered with supportive treatment. hantavirus infecti ...	2011	20736852
detection of crimean-congo hemorrhagic fever, hanta, and sandfly fever viruses by real-time rt-pcr.	the development of sensitive and specific nucleic acid diagnostic assays for viral pathogens is essential for proper medical intervention. this chapter describes four fluorescence-based pcr assays to detect the crimean-congo hemorrhagic fever (cchfv), andes (andv), hantaan (hanv), and sandfly fever sicilian (sfsv) viruses. these assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for cchfv and sfsv and the glycoprotein gene for andv and hanv. all four a ...	2011	21116810
environmental risk factors for haemorrhagic fever with renal syndrome in a french new epidemic area.	summaryin france, haemorrhagic fever with renal syndrome (hfrs) is endemic along the belgian border. however, this rodent-borne zoonosis caused by the puumala virus has recently spread south to the franche-comté region. we investigated the space-time distribution of hfrs and evaluated the influence of environmental factors that drive the hantavirus reservoir abundance and/or the disease transmission in this area. a scan test clearly indicated space-time clustering, highlighting a single-year (20 ...	2011	20822577
genetic evidence for the presence of two distinct hantaviruses associated with apodemus mice in croatia and analysis of local strains.	in europe, dobrava-belgrade (dobv), saaremaa (saav), and puumala (puuv) viruses are known to cause hemorrhagic fever with renal syndrome (hfrs). all three hantaviruses are now found in croatia. lung tissue samples of 315 apodemus mice trapped in 2003-2004 were screened for the presence of hantaviral n-ag and 20 mice (6.3%) were found either strongly positive or weak/suspected-positive. partial sequences of hantavirus m and s segments were recovered by rt-pcr from six mice and subjected to (phylo ...	2011	21108346
impacts of biodiversity on the emergence and transmission of infectious diseases.	current unprecedented declines in biodiversity reduce the ability of ecological communities to provide many fundamental ecosystem services. here we evaluate evidence that reduced biodiversity affects the transmission of infectious diseases of humans, other animals and plants. in principle, loss of biodiversity could either increase or decrease disease transmission. however, mounting evidence indicates that biodiversity loss frequently increases disease transmission. in contrast, areas of natural ...	2010	21124449
temporal variation in individual factors associated with hantavirus infection in bank voles during an epizootic: implications for puumala virus transmission dynamics.	abstract puumala virus (puuv), the causal agent of nephropathia epidemica in humans, is one of the many hantaviruses included in the list of emerging pathogens. hantavirus infection is not distributed evenly among puuv reservoir hosts (i.e., bank voles [myodes glareolus]). besides environmental factors and local population features, individual characteristics play an important role in vole puuv infection risk. identifying the relative importance of these individual characteristics can provide cr ...	2010	21142469
[construction and immunogenic study of recombinant adenovirus containing chimeric gene g2s0.7 and ctl epitopes of hantaan virus].	to express g2 fragment of m segment and 0.7 kb fragment of s segment and several ctl epitopes of s segment in adenovirus expression system and investigate the immunological properties of hantaan virus chimeric gene.	2010	21055343
circulation of hantaviruses in the influence area of the cuiabá-santarém highway.	we describe evidence of circulation of hantaviruses in the influence area of the santarém-cuiabá highway (br-163) in the brazilian amazon through the prevalence of specific antibodies against hantaviruses in inhabitants living in four municipalities of this area: novo progresso (2.16%) and trairão (4.37%), in state of pará (pa), and gua-rantã do norte (4.74%) and marcelândia (9.43%), in state of mato grosso. we also demonstrate the ongoing association between castelo dos sonhos virus (casv) and ...	2010	20835614
pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus).	the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ...	2010	20455779
electron cryotomography of tula hantavirus suggests a unique assembly paradigm for enveloped viruses.	hantaviruses (family bunyaviridae) are rodent-borne emerging viruses that cause a serious, worldwide threat to human health. hantavirus diseases include hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. virions are enveloped and contain a tripartite single-stranded negative-sense rna genome. two types of glycoproteins, g(n) and g(c), are embedded in the viral membrane and form protrusions, or "spikes." the membrane encloses a ribonucleoprotein core, which consists of ...	2010	20219926
small molecule inhibitors of hantavirus infection.	hantaviruses use α(v)β(3) integrins on the surface of human host cells as a gateway to invasion, hence compounds that target this receptor may be used as antiviral agents. to accomplish this aim, new peptidomimetic compounds were selected based on similarity to a cyclic peptide known to bind the α(v)β(3) receptor. this first round of biological screening identified peptidomimetic molecules which were effective hantavirus inhibitors in the low micromolar range, two thousand times more potent than ...	2010	20951038
structural studies of hantaan virus.	hantaan virus is the prototypic member of the hantavirus genus within the family bunyaviridae and is a causative agent of the potentially fatal hemorrhagic fever with renal syndrome. the bunyaviridae are a family of negative-sense rna viruses with three-part segmented genomes. virions are enveloped and decorated with spikes derived from a pair of glycoproteins (gn and gc). here, we present cryo-electron tomography and single-particle cryo-electron microscopy studies of hantaan virus virions. we ...	2010	21068243
the relative abundance of deer mice with antibody to sin nombre virus corresponds to the occurrence of hantavirus pulmonary syndrome in nearby humans.	abstract sin nombre virus (snv) is the principal cause of hantavirus pulmonary syndrome (hps) in the united states and deer mice (peromyscus maniculatus) are its principal rodent host, and thus the natural cycle of the virus is related to the occurrence of hps. prevalence of rodent infection appears to be associated with fluctuations in deer mouse populations and, indirectly, with timing and amount of precipitation, a complex of biologic events. given that rodent population abundances fluctuate, ...	2010	20954865
in vivo kinetics and biodistribution of a hantaan virus dna vaccine after intramuscular injection in mice.	to study the kinetics in vivo of a hantaan virus dna vaccine, we constructed a fusion dna vaccine, pegfp/s, by cloning the s segment of hantavirus into the vector, pegfp-c1, which encodes green fluorescent protein egfp. in this report, we provide evidence that pegfp/s was distributed and persistently expressed for more than 60 days in several organs after inoculation. our findings suggest that the persistent immune responses induced by a hantaan virus dna vaccine are likely due to the plasmid pe ...	2010	20960291
interaction of hantavirus nucleocapsid protein with ribosomal protein s19.	hantaviruses, members of the bunyaviridae family, are emerging category a pathogens that initiate the translation of their capped mrnas by a novel mechanism mediated by viral nucleocapsid protein (n). n specifically binds to the mrna 5' m7g cap and 40s ribosomal subunit, a complex of 18s rrna and multiple ribosomal proteins. here, we show that n specifically interacts with the ribosomal protein s19 (rps19), located at the head region of the 40s subunit. we suggest that this n-rps19 interaction f ...	2010	20844026
pathogenicity and virulence of the present hantaviruses in bosnia and herzegovina: the impact on renal function.	dobrava (dobv) and puumala (puuv) viruses are endemic throughout the balkans and cause haemorrhagic fever with renal syndrome (hfrs). the aim of this study was to assess the impact of two different hantaviruses on renal function in hfrs patients during the acute stage of illness. we also aimed to assess the dobv and puuv distribution between symptomatic hfrs patients and asymptomatic hantavirus antibody-positive subjects. the study included 264 symptomatic hfrs patients and 63 asymptomatic hanta ...	2010	20972691
characterization of a natural focus of puumala hantavirus infection in the czech republic.	hantaviruses are rna viruses of the bunyaviridae family, represented in the czech republic by three genospecies: dobrava-belgrade, puumala and tula. they persist in natural foci of infection. in 2004 to 2009, a local outbreak with 18 reported cases of nephropathia epidemica caused by puumala hantavirus occurred in the sumava mountains and foothills and was spacially associated with another outbreak in lower bavaria, germany. in the jeleni locality in the sumava mountains at 880 m above sea level ...	2010	20939264
pathogenic landscapes: interactions between land, people, disease vectors, and their animal hosts.	landscape attributes influence spatial variations in disease risk or incidence. we present a review of the key findings from eight case studies that we conducted in europe and west africa on the impact of land changes on emerging or re-emerging vector-borne diseases and/or zoonoses. the case studies concern west nile virus transmission in senegal, tick-borne encephalitis incidence in latvia, sandfly abundance in the french pyrenees, rift valley fever in the ferlo (senegal), west nile fever and t ...	2010	20979609
syndromic surveillance: etiologic study of acute febrile illness in dengue suspicious cases with negative serology. brazil, federal district, 2008.	with the aim of identifying the etiology of acute febrile illness in patients suspected of having dengue, yet with non reagent serum, a descriptive study was conducted with 144 people using secondary serum samples collected during convalescence. the study was conducted between january and may of 2008. all the exams were re-tested for dengue, which was confirmed in 11.8% (n = 17); the samples that remained negative for dengue (n = 127) were tested for rubella, with 3.9% (n = 5) positive results. ...	2010	21049226
smoking and other risk factors for hantavirus infections: the whole story.		2010	20875215
[morphological findings in fatal cases of hantavirus cardiopulmonary syndrome. report of 7 autopsies].	hantavirus cardiopulmonary syndrome (hcps) is an acute infectious disease characterized by sudden cardiorespiratory failure and high mortality, caused by a rna virus of the genus hantavirus, family bunyaviridae, 15% of chilean cases have been detected in the araucania region.	2010	21186503
dobrava-belgrade hantavirus infection mimics acute appendicitis.	dobrava-belgrade hantavirus infection mimicked acute appendicitis in a patient suffering from hemorrhagic fever with renal syndrome in hungary. the 27-year-old man was admitted to the local hospital with severe abdominal pain localized mainly at the right lower quadrant of the abdomen and with fever, nausea, vomiting and bloody diarrhea. based on these findings supported by computerized tomography acute perforated appendicitis was suspected and an explorative laparatomy was performed, which did ...	2010	21075678
tracking hantavirus nucleocapsid protein using intracellular antibodies.	hantavirus nucleocapsid (n) protein is a multifunctional viral macromolecule involved in multiple stages of the viral replication cycle. the intracellular trafficking of n protein during virus assembly remains unclear.	2010	21092325
seroprevalence of hantavirus antibodies among chronic hemodialysis patients in the czech republic.	a total of 348 serum samples were collected from 301 hemodialysis patients with chronic renal failure of four healthcare settings in western bohemia. the sera were screened for the presence of hantavirus antibodies using elisa kits (progen biotechnik gmbh) with hantaan and puumala antigens. specific anti-puumala antibodies were detected in five patients (1.7%). although hantaviruses are known to cause primarily acute renal damage (interstitial nephritis) in eurasia, chronic effects of hantavirus ...	2010	21105569
characterization of two substrains of puumala virus that show phenotypes that are different from each other and from the original strain.	hantaviruses, the causative agents of two emerging diseases, are negative-stranded rna viruses with a tripartite genome. we isolated two substrains from a parental strain of puumala hantavirus (puuv-pa), puuv-small (puuv-sm) and puuv-large (puuv-la), named after their focus size when titrated. the two isolates were sequenced; this revealed differences at two positions in the nucleocapsid protein and two positions in the rna-dependent rna polymerase, but the glycoproteins were identical. we also ...	2010	21106742
hantavirus infection: a neglected diagnosis in thrombocytopenia and fever?	thrombocytopenia, fever, and acute renal failure are characteristic features of nephropathia epidemica, the predominant hantavirus infection in europe. however, clinical presentation and blood cell counts may point to other disorders, such as a hematologic disease, particularly when impairment of renal function is not evident. this differential diagnosis often results in further extensive and unnecessary testing. we describe 3 patients with hantavirus infection with no renal failure, in whom a h ...	2010	21037045
[hemorrhagic fever with renal syndrome during pregnancy: case report].	hemorrhagic fever with renal syndrome (hfwrs) is an acute infectious disease with abrupt onset, high fever, renal failure and frequent hemorragies. hfwrs during the pregnancy has relatively low occurrence, with only few described cases mostly in the region of the former soviet union. although, according to the data from the literature, the disease is less severe during the pregnancy, the eclampsy, stillbirth and fetal infection were also described.	2010	21053473
andes virus regulation of cellular micrornas contributes to hantavirus-induced endothelial cell permeability.	hantaviruses infect human endothelial cells (ecs) and cause two diseases marked by vascular permeability defects, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). vascular permeability occurs in the absence of ec lysis, suggesting that hantaviruses alter normal ec fluid barrier functions. ecs infected by pathogenic hantaviruses are hyperresponsive to vascular endothelial growth factor (vegf), and this alters the fluid barrier function of ec adherens junctions ...	2010	20844033
aromatic and polar residues spanning the candidate fusion peptide of the andes virus gc protein are essential for membrane fusion and infection.	hantaviruses infect human cells through cell attachment and subsequent fusion of viral and cellular membranes at low ph. this largely unknown entry process is mediated by the gn and gc glycoproteins, anchored at the viral envelope membrane. performing bioinformatic analysis and peptide-liposome-binding assays we suggested in a former report that gc of andes virus (andv) and other hantaviruses corresponds to the viral fusion protein sharing characteristics with class ii fusion proteins. to gain i ...	2010	21123541
[hantavirus infection: two case reports from a province in the eastern blacksea region, turkey.]	hantaviruses which are the members of bunyaviridae, differ from other members of this family since they are transmitted to humans by rodents. more than 200.000 cases of hantavirus infections are reported annually worldwide. hantaviruses can lead to two different types of infection in humans, namely, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hfrs is the most common type of hantavirus infection in europe and asia and the most common virus types are dobra ...	2010	21063999
genetic characterization of hantaviruses associated with sigmodontine rodents in an endemic area for hantavirus pulmonary syndrome in southern brazil.	an ecological assessment of reservoir species was conducted in a rural area (jaborá) in the mid-west of the state of santa catarina in southern brazil, where hantavirus pulmonary syndrome is endemic, to evaluate the prevalence of hantavirus infection in wild rodents. blood and tissue samples were collected from 507 rodents during seven field trips from march 2004 to april 2006. some of the animals were karyotyped to confirm morphological identification. phylogenetic reconstructions of rodent spe ...	2010	21138380
evidence of human hantavirus infection and zoonotic investigation of hantavirus prevalence in rodents in western java, indonesia.	abstract during febrile surveillance in the western java city of bandung, indonesia, a patient with clinical symptoms consistent with hantavirus infection was found to have elevated titers of hantavirus-specific immunoglobulin m (igm) and igg antibodies. a subsequent epizoological investigation demonstrated a higher prevalence of hantavirus igg antibodies in rodents trapped in the vicinity of the patient's home compared with rodents from a control area (13.2% vs. 4.7%, p = 0.036). the old world ...	2010	21142967
[nephropathia epidemica caused by puumala hantavirus infection: a case report from the outpatient sector].	a 42-year-old man without pre-existing health problems presented to his family practitioner in baden-württemberg with fever (up to 39˚ c for two days), headache, abdominal pain, back pain and aching limbs. ten days before, he had done heavy gardening work. he felt diffuse pain on abdominal palpation. the physical examination of the skin, lymph nodes, the heart, the lungs and the oral mucosa was unremarkable.	2010	21140328
characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples.	monoclonal antibodies are important tools for various applications in hantavirus diagnostics. recently, we generated puumala virus (puuv)-reactive monoclonal antibodies (mabs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (vlps) harbouring the 120-amino-acid-long amino-terminal region of the puuv nucleocapsid (n) protein. here, we describe the generation of two mabs by co-immunisation of mice with hexahistidine-tagged full-length n proteins of sin nombre virus ( ...	2010	21161552
small mammal-associated zoonoses.		2010	20795915
hantaviruses and their hosts in europe: reservoirs here and there, but not everywhere?	five hantaviruses are known to circulate among rodents in europe, and at least two among insectivores. four (dobrava, saaremaa, seoul, and puumala [puuv] viruses) are clearly associated with hemorrhagic fever with renal syndrome (hfrs). puuv, the most common etiological agent of hfrs in europe, is carried by the bank vole (myodes glareolus), one of the most widespread and abundant mammal species in europe. this host-virus system is among hantaviruses also the most studied one in europe. however, ...	2010	20795916
diagnosis of hantavirus infection in humans and rodents in ribeirão preto, state of são paulo, brazil.	hantavirus pulmonary and cardiovascular syndrome (hpcs) is an emerging serious disease in the americas. hantaviruses (bunyaviridae) are the causative agents of this syndrome and are mainly transmitted through inhalation of aerosols containing the excreta of wild rodents. in the ribeirão preto region (state of são paulo, brazil), hpcs has been reported since 1998, caused by the araraquara virus (arav), for which necromys lasiurus is the rodent reservoir. this study aimed to show diagnostic result ...	2010	20802928
long-term patterns of immune investment by wild deer mice infected with sin nombre virus.	immunocompetence of animals fluctuates seasonally, however, there is little consensus on the cause of these fluctuations. some studies have suggested that these patterns are influenced by changes in reproductive condition, whereas others have suggested that differences result from seasonal variations in energy expenditures. the objective of our study was to examine these contrasting views of immunity by evaluating seasonal patterns of immune response and reproduction in wild populations of deer ...	2010	20695811
genetic reassortment between high-virulent and low-virulent dobrava-belgrade virus strains.	the tri-segmented rna genome of hantaviruses facilitates genetic reassortment by segment swapping when cells are co-infected with different virus strains. we found efficient in vitro reassortment between members of two different genetic lineages of the dobrava-belgrade virus species, the weakly virulent dobv-aa and highly virulent dobv-af. in all reassortants, s and l segments originated from the same parental strain, and only the m segment was exchanged. to identify functional differences betwe ...	2010	20734125
the 3' untranslated region of the andes hantavirus small mrna functionally replaces the poly(a) tail and stimulates cap-dependent translation initiation from the viral mrna.	in the process of translation of eukaryotic mrnas, the 5' cap and the 3' poly(a) tail interact synergistically to stimulate protein synthesis. unlike its cellular counterparts, the small mrna (smrna) of andes hantavirus (andv), a member of the bunyaviridae, lacks a 3' poly(a) tail. here we report that the 3' untranslated region (3'utr) of the andv smrna functionally replaces a poly(a) tail and synergistically stimulates cap-dependent translation initiation from the viral mrna. stimulation of tra ...	2010	20660206
andes virus disrupts the endothelial cell barrier by induction of vascular endothelial growth factor and downregulation of ve-cadherin.	hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs) are severe diseases associated with hantavirus infection. high levels of virus replication occur in microvascular endothelial cells but without a virus-induced cytopathic effect. however, virus infection results in microvascular leakage, which is the hallmark of these diseases. ve-cadherin is a major component of adherens junctions, and its interaction with the vascular endothelial growth factor (vegf) receptor, ...	2010	20810734
extinction of refugia of hantavirus infection in a spatially heterogeneous environment.	we predict an abrupt observable transition, on the basis of numerical studies, of hantavirus infection in terrain characterized by spatially dependent environmental resources. the underlying framework of the analysis is that of fisher equations with an internal degree of freedom, the state of infection. the unexpected prediction is of the sudden disappearance of refugia of infection in spite of the existence of supercritical (favorable) food resources, brought about by reduction of their spatial ...	2010	20866661
hantavirus pulmonary syndrome in argentina, 1995-2008.	we report a large case series of hantavirus pulmonary syndrome (hps) in argentina that was confirmed by laboratory results from 1995 through 2008. the geographic and temporal distribution of cases by age, sex, fatality rate, and risk factors for hps was analyzed. a total of 710 cases were unequally distributed among 4 of the 5 argentine regions. different case-fatality rates were observed for each affected region, with a maximum rate of 40.5%. the male-to-female ratio for hps case-patients was 3 ...	2010	21122213
hantaviruses and hantavirus pulmonary syndrome, maranhao, brazil.	to confirm circulation of anajatuba virus in maranhao, brazil, we conducted a serologic survey (immunoglobulin g elisa) and phylogenetic studies (nucleocapsid gene sequences) of hantaviruses from wild rodents and persons with hantavirus pulmonary syndrome. this virus is transmitted by oligoryzomys fornesi rodents and is responsible for hantavirus pulmonary syndrome in this region.	2010	21122229
antagonism of type i interferon responses by new world hantaviruses.	evasion of interferon (ifn)-mediated antiviral signaling is a common defense strategy for pathogenic rna viruses. to date, research on ifn antagonism by hantaviruses is limited and has focused on only a subset of the numerous recognized hantavirus species. the host ifn response has two phases, an initiation phase, resulting in the induction of alpha/beta ifn (ifn-α/β), and an amplification phase, whereby ifn-α/β signals through the jak/stat pathway, resulting in the establishment of the cellular ...	2010	20844031
acute undifferentiated febrile illness in adult hospitalized patients: the disease spectrum and diagnostic predictors - an experience from a tertiary care hospital in south india.	local prevalences of individual diseases influence the prioritization of the differential diagnoses of a clinical syndrome of acute undifferentiated febrile illness (afi). this study was conducted in order to delineate the aetiology of afi that present to a tertiary hospital in southern india and to describe disease-specific clinical profiles. an 1-year prospective, observational study was conducted in adults (age >16 years) who presented with an undifferentiated febrile illness of duration 5-21 ...	2010	20870680
early diagnosis of hantavirus infection by family doctors can reduce inappropriate antibiotic use and hospitalization.	hantavirus infections are emerging infections that cause either hantavirus pulmonary syndrome or haemorrhagic fever with renal syndrome (hfrs). a recent swedish outbreak of nephropathia epidemica, a european hfrs, was analysed to study the patient flow and clinical picture and to investigate the value of an early diagnosis in general practice. design. in a retrospective design, medical records of verified cases of hantavirus infection were studied.	2010	20642397
confirmation of choclo virus as the cause of hantavirus cardiopulmonary syndrome and high serum antibody prevalence in panama.	choclo virus (chov) was described in sigmodontine rodents, oligoryzomys fulvescens, and humans during an outbreak of hantavirus cardiopulmonary syndrome (hcps) in 1999-2000 in western panama. although hcps is rare, hantavirus-specific serum antibody prevalence among the general population is high suggesting that chov may cause many mild or asymptomatic infections. the goals of this study were to confirm the role of chov in hcps and in the frequently detected serum antibody and to establish the p ...	2010	20648614
temporal and spatial host abundance and prevalence of andes hantavirus in southern argentina.	andes virus (and) is a hantavirus hosted by the sigmodontine rodent oligoryzomys longicaudatus in southern argentina, where it is responsible for most cases of hantavirus pulmonary syndrome (hps). our study provides data about the spatial variation in abundance of the rodent host of and hantavirus. we report results of a longitudinal study performed in a locality of the andean region of chubut province. from november 2003 (spring) to july 2006 (winter), o. longicaudatus was the most common speci ...	2010	20645121
switch to high-level virus replication and hla class i upregulation in differentiating megakaryocytic cells after infection with pathogenic hantavirus.	hantaan virus (htnv), the prototype member of the hantavirus genus in the family bunyaviridae, causes hemorrhagic fever with renal syndrome (hfrs) in humans. hemorrhage is due to endothelial barrier damage and a sharp decrease in platelet counts. the mechanisms underlying htnv-associated acute thrombocytopenia have not been elucidated so far. platelets are produced by mature megakaryocytes that develop during megakaryopoiesis. in this study, we show that htnv targets megakaryocytic cells whereas ...	2010	20673746
occupation-related respiratory infections revisited.	occupational pulmonary infectious diseases include tuberculosis (tb) and many viral pathogens, including influenza, coronavirus (severe acute respiratory syndrome or sars), varicella, respiratory syncytial virus, and hantavirus. this review focuses on tb, influenza, and sars, because the published literature is extensive for these 3 infections. the lessons from these 3 are relevant for all nosocomial pulmonary infectious diseases.	2010	20674797
tnf-α expression and promoter sequences reflect the balance of tolerance/resistance to puumala hantavirus infection in european bank vole populations.	the tumor necrosis factor-alpha (tnf-α) influences the ability to limit parasite infection but its over-production might result in inflammatory disorders. the level of tnf-α gene expression could thus mediate a balance of tolerance/resistance to infections. this study focused on puumala hantavirus (puuv) infection in its rodent host, the bank vole (myodes glareolus). in humans, puuv is responsible of a mild form of hemorrhagic fever with renal syndrome, nephropathia epidemica (ne). the severity ...	2010	20691810
development of a lentiviral vector system to study the role of the andes virus glycoproteins.	to infect target cells, enveloped viruses use their virion surface proteins to direct cell attachment and subsequent entry via virus-cell membrane fusion. how hantaviruses enter cells has been largely unexplored. to study early steps of andes virus (andv) cell infection, a lentiviral vector system was developed based on a simian immunodeficiency virus (siv) vector pseudotyped with the andv-gn/gc envelope glycoproteins. the incorporation of gn and gc onto siv-derived vector particles was assessed ...	2010	20619306
a case report of crescentic glomerulonephritis associated with hantaan virus infection.	although various glomerular diseases in hantavirus infection have been reported, an association between hantavirus infection and crescentic glomerulonephritis has not been described. herein, we report a case of immune complex-mediated crescentic glomerulonephritis in a 70-year-old man with hantaan virus infection.	2010	20466689
generation of e3-deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences.	seoul virus (seov) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. the medium genome segment encodes the gn/gc glycoproteins of seov, which form the major structural part of the virus envelope. gc and/or gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. in this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gc protein of seov w ...	2010	20181748