| [pathogenesis of hemorrhagic fever]. | | 2001 | 11496775 |
| [hantavirus-related hemorrhagic fever in europe]. | | 2001 | 11496776 |
| [detection for anti-hantavirus igm in patient serum with silver enhanced dot immunogold filtration assay]. | to establish a more rapid and simple method for detecting anti-hantavirus igm in patient' s serum. | 2000 | 11498694 |
| hantavirus pulmonary syndrome due to andes virus in temuco, chile: clinical experience with 16 adults. | to describe the clinical features and laboratory abnormalities of 16 adults with confirmed hantavirus pulmonary syndrome (hps) due to andes virus in temuco, chile. | 2001 | 11502657 |
| [the partial nucleotide sequence of l segment of hantavirus strain]. | to study the nucleotide sequence of l segment of hantavirus strain a9. | 2000 | 11503036 |
| hantavirus update on an emerging--and lethal--infectious disease. | | 2000 | 11503410 |
| longitudinal studies of sin nombre virus in deer mouse-dominated ecosystems of montana. | sin nombre virus (snv), hosted by the deer mouse (peromyscus maniculatus), is the primary etiologic agent of hantavirus pulmonary syndrome (hps) in north america. to improve our understanding of the epidemiology of hps in the western united states, we conducted studies of population dynamics and snv antibody prevalence in deer mouse populations for 6 years on 12 mark-recapture grids in montana. monthly numbers of deer mice ranged from zero to over 170 on 1-hectare grids. snv antibody prevalence ... | 2001 | 11504405 |
| sex differences in immune responses and viral shedding following seoul virus infection in norway rats. | in the field, male rodents are more frequently infected with hantaviruses than females. this study examined whether patterns of immune responses against hantavirus differed between the sexes. male and female long evans rats (rattus norvegicus) were inoculated with seoul virus, and antibody and cytokine responses, as well as virus shedding were assessed. males were more likely to shed virus in saliva, to shed virus through multiple routes (saliva, urine, and feces), and to have viral rna in the s ... | 2001 | 11504409 |
| dna vaccination with the hantaan virus m gene protects hamsters against three of four hfrs hantaviruses and elicits a high-titer neutralizing antibody response in rhesus monkeys. | four hantaviruses-hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv) and puumala virus-are known to cause hemorrhagic fever with renal syndrome (hfrs) in europe and asia. htnv causes the most severe form of hfrs (5 to 15% case-fatality rate) and afflicts tens of thousands of people annually. previously, we demonstrated that dna vaccination with a plasmid expressing the seov m gene elicited neutralizing antibodies and protected hamsters against infection with seov and htnv. here, we r ... | 2001 | 11507192 |
| hbv core particles as a carrier for b cell/t cell epitopes. | in the middle 80s, recombinant hepatitis b virus cores (hbc) gave onset to icosahedral virus-like particles (vlps) as a basic class of non-infectious carriers of foreign immunological epitopes. the recombinant hbc particles were used to display immunodominant epitopes of hepatitis b, c, and e virus, human rhinovirus, papillomavirus, hantavirus, and influenza virus, human and simian immunodeficiency virus, bovine and feline leukemia virus, foot-and-mouth disease virus, murine cytomegalovirus and ... | 2001 | 11509871 |
| a chemiluminescence detection method of hantaviral antigens in neutralisation assays and inhibitor studies. | a protocol for chemiluminescence detection of hantaviral antigens in infected cell foci is described. this focus detection is based on the conversion of a substrate into a luminescent product by peroxidase-antibody conjugates; the emitted light of infected cell foci can easily be recorded by autoradiography or video imaging providing a hard copy for documentation. the main advantage of this method as compared to conventional immunochemical staining is a higher detection sensitivity due to the in ... | 2001 | 11516485 |
| virology. finally, a handle on the hantaviruses. | | 2001 | 11520958 |
| hemorrhagic fever with renal syndrome: diagnostic problems with a known disease. | hemorrhagic fever with renal syndrome (hfrs), caused by different hantaviruses, is a distinct clinical syndrome endemic in several parts of asia and europe. however, the clinical picture can sometimes be indistinguishable from that of other infectious or noninfectious diseases. in this report we describe a clinical case, which is a rare occurrence but is a prime example of the difficulties in the diagnosis of hfrs in areas with a low prevalence of the disease. | 2001 | 11526193 |
| from the centers for disease control and prevention. hantavirus pulmonary syndrome--vermont, 2000. | | 2001 | 11529243 |
| epidemiology and epizootiology of hantavirus infection in japan. | hantaviruses cause two severe human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). various rodent species act as animal reservoirs for hantavirus. in japan, urban rat- (rattus norvegicus) and laboratory rat-derived human infections were reported during the 1960s and 1970s-1984, respectively. although no human cases of infection have been reported since 1984, infected urban rats have been found throughout japan, and infected grey red-backed voles ( ... | 2001 | 11544398 |
| hantavirus infection complicated with arf and spontaneous perinephric haemorrhage. | | 2001 | 11549914 |
| hantavirus pulmonary syndrome in anajatuba, maranhão, brazil. | the authors report a confirmed case of hantavirus pulmonary syndrome in the rural area of the municipality of anajatuba, state of maranhão. two other suspected cases from the same region are also described. the confirmed case involved a previously healthy young woman who died with signs and symptoms of acute respiratory insufficiency 5 days after presenting fever, myalgia and a dry cough. the patient was a student who was helping her parents with work in the fields; it was a habit of the family ... | 2001 | 11558007 |
| purification and characterization of the sin nombre virus nucleocapsid protein expressed in escherichia coli. | sin nombre virus is a member of the hantavirus genus, family bunyaviridae, and is an etiologic agent of hantavirus pulmonary syndrome. the hantavirus nucleocapsid (n) protein plays an important role in the encapsidation and assembly of the viral negative-sense genomic rna. the sin nombre n protein was expressed as a c-terminal hexahistidine fusion in escherichia coli and initially purified by nickel-affinity chromatography. we developed methods to extract the soluble fraction and to solubilize t ... | 2001 | 11570855 |
| epizootiological survey of hantavirus among rodent species in ningxia hui autonomous province, china. | hantaviral antibodies were detected in the sera from apodemus (a.) agrarius and a. peninsulae captured in ningxia province, china by several different serological diagnostic methods. a total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (ifa). among them, 19 of 191 (9.9%) sera of a. agrarius and 1 of 13 (7.7%) sera of a. peninsulae were positive for hantaviral antibodies. the other species (rattus norvegicus, mus ... | 2001 | 11590918 |
| epidemiological progresses of hemorrhagic fever with renal syndrome in china. | to summarize the major achievements of chinese work on the epidemiology and prevention of hemorrhagic fever with renal syndrome (hfrs) in recent years, and to give a general review on the present situation of hfrs in china. | 1999 | 11593522 |
| comparison of a new immunochromatographic rapid test with a commercial eia for the detection of puumala virus specific igm antibodies. | hantaviruses are associated with two human diseases: haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). puumala virus (puuv), which is one of the hantaviruses, is a causative agent of nephropathia epidemica (ne), a mild form of hfrs. | 2001 | 11595586 |
| human immune response to puumala virus glycoproteins and nucleocapsid protein expressed in mammalian cells. | puumala hantavirus (puuv) glycoproteins g1 and g2 and nucleocapsid protein (n) were expressed in bhk-21 cells by transfection of a plasmid producing a recombinant alphavirus replicon. coexpression of g1 and g2 from separate constructs seemed to be important for the optimal folding of the glycoproteins, as evaluated by a panel of mabs detecting conformational epitopes. to evaluate the human antibody response against recombinant g1, g2 and n, several panels of sera were tested by immunofluorescenc ... | 2001 | 11596100 |
| hantavirus infections in greece--an update. | hantaviruses are emerging viruses spread worldwide and cause hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). two pathogenic hantaviruses are present in greece: dobrava/belgrade virus (dobv) and puumala virus (puuv). the predominant one is dobv, carried in greece by the rodent apodemus flavicollis. most human infections are sporadic, but epidemic outbreaks were also described. the severity of the disease ranges from a mild or moderate form to severe and fatal ... | 2001 | 11599695 |
| a lethal disease model for hantavirus pulmonary syndrome. | hantaviruses are associated with two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). development of vaccines and therapies to prevent and treat hfrs and hps have been hampered by the absence of a practical animal model. here we report that andes virus (andv), a south american hantavirus, is highly lethal in adult syrian hamsters. the characteristics of the disease in hamsters, including the incubation period, symptoms of rapidly progressing r ... | 2001 | 11601912 |
| anne boleyn, the sweating sickness, and the hantavirus: a review of an old disease with a modern interpretation. | | 1998 | 11619876 |
| expression of integrin alphavbeta3 in pig, dog and cattle. | the alphavbeta3 integrin, also known as vitronectin receptor, is an adhesive glycoprotein that promotes angiogenesis in the embryo and tumors such as melanoma. integrin alphavbeta3 is one of the receptors for adenovirus and hantavirus. there is little information on the constitutive expression of this integrin especially in animal species that are used for biomedical research. we used light and electron microscope immunocytochemistry and western blots to determine integrin alphavbeta3 expression ... | 2001 | 11642723 |
| genotyping and preparation of the recombinant nucleocapsid protein antigen of hantavirus. | to identify new recombinant antigens with potential for diagnosis of hemorrhagic fever with renal syndrome (hfrs) and establish reverse transcription-polymerase chain reaction-restriction fragments length polymorphism (rt-pcr-rflp) for genotyping of hantavirus. | 2001 | 11677760 |
| seasonal variation in prevalence of antibody to hantaviruses in rodents from southern argentina. | we conducted a small mammal trapping study to investigate temporal variation in prevalence of infection in hantavirus reservoir populations in the patagonian andes mountain range, rio negro province, argentina. rodent blood samples collected in natural and periurban habitats and at the home of an hantavirus pulmonary syndrome (hps) case patient were analysed by enzyme-linked immunosorbent assay. organ tissue samples were tested by polymerase chain reaction (pcr) and nucleotide sequence analysis. ... | 2001 | 11679129 |
| [hantavirus infection in bank voles in the natural reservoir. part 1. characteristics of an infection process in bank voles]. | the specific features of hantavirus infection in naturally infected bank voles (clethrionomys glareolus), the principal host of hantavirus of the serotype puumala, were studied during long-term observation of individually marked animals in the active focus of hemorrhagic fever with renal syndrome (hfrs) in the south of udmurtia. the infection time in the bank voles was defined by paired serum seroconversion tests. in the natural focus, hantavirus was shown to cause asymptomatic persistent infect ... | 2001 | 11680366 |
| hantavirus infections in europe. | | 2001 | 11680383 |
| the hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide waasa. | the medium segment of the tripartite negative-stranded rna genome of hantaviruses encodes for the predicted glycoprotein precursor gpc. we have demonstrated here the expression of the glycoprotein precursor of hantaan virus following transfection of mammalian cells. the cleavage of the precursor into the glycoproteins g1 and g2 followed the rules for signal peptides and seemed to occur directly at the pentapeptide motif "waasa." our data indicate that the signal peptidase complex is responsible ... | 2001 | 11689045 |
| molecular evolution of puumala hantavirus. | puumala virus (puuv) is a negative-stranded rna virus in the genus hantavirus, family bunyaviridae. in this study, detailed phylogenetic analysis was performed on 42 complete s segment sequences of puuv originated from several european countries, russia, and japan, the largest set available thus far for hantaviruses. the results show that puuv sequences form seven distinct and well-supported genetic lineages; within these lineages, geographical clustering of genetic variants is observed. the ove ... | 2001 | 11689661 |
| [hantavirus infection epidemiology in belgium]. | in europe, puumala (puu) is a hantavirus responsible for a human disease called nephropathia epidemica and its natural reservoir is the red bank vole, (clethrionomys glareolus). although the population densities and the prevalence rates of infection were high in red bank voles in southern belgium during the 1996 and 1999 epidemic years, the percentages of infected rodents were low in 1997 and 1998, when only a few positive sites were found. antibodies against puu virus were mainly detected in th ... | 2001 | 11697188 |
| hantavirus infections and their prevention. | hantaviruses are rodent-borne bunyaviruses which cause haemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome in humans. this review covers the host interactions of the viruses, including the rodent reservoirs, the clinical outcome of human infections as well as the pathogenesis and laboratory diagnosis of infections. the current stage in prophylaxis and therapy of hantaviral diseases is described and different approaches in vaccine development are discussed. | 2001 | 11709294 |
| rapid presumptive diagnosis of hantavirus cardiopulmonary syndrome by peripheral blood smear review. | hantavirus cardiopulmonary syndrome (hcps) is a rare but frequently lethal acute zoonotic viral infection in rural north america. the rapidity of progression from febrile prodrome to cardiogenic shock and noncardiogenic pulmonary edema requiring intensive care creates high diagnostic urgency and a need for a rapid screening tool. in this retrospective cohort study, 2 pathologists scored blinded peripheral blood smears from 52 patients with hcps and 128 seronegative patients referred for diagnosi ... | 2001 | 11710682 |
| [hemorrhagic fever with renal syndrome]. | puumala hantavirus is the most common hantavirus infection in western europe. the causative agent, puumala virus, is a member of the hantavirus genus in the bunyaviridae family. the natural hosts of hantaviruses are chronically, but asymptomatic infected rodents, which transmit the virus to human in their excretions. puumala virus is carried by the bank vole, clethrionomys glareolus. hemorrhagic fever with renal syndrome (hfrs) caused by puumala virus in france or belgium is very similar to the ... | 2001 | 11715607 |
| estimated incubation period for hantavirus pulmonary syndrome. | | 2001 | 11716089 |
| mesangiocapillary glomerulonephritis caused by puumala hantavirus infection. | nephropathia epidemica induced by puumala hantavirus typically causes acute reversible renal function impairment. a typical renal biopsy finding is acute tubulointerstitial nephritis with slight glomerular mesangial changes. we describe here 5 patients who developed the nephrotic syndrome during the convalescent phase of an otherwise typical acute febrile nephropathia epidemica. renal biopsy of all patients disclosed type i mesangiocapillary glomerulonephritis (mcgn). a clinical remission of the ... | 2001 | 11721157 |
| first molecular evidence for puumala hantavirus in slovakia. | we report on the first puumala hantavirus nucleotide sequence (strain opina-916) amplified from a bank vole trapped in slovakia, central europe. phylogenetic analysis of the s-segment sequence grouped the virus within the western/central european sublineage of puumala virus. in the neighborhood of the rodent trapping site two cases of human infection by the puumala virus were verified. | 2001 | 11724269 |
| wild-type puumala hantavirus infection induces cytokines, c-reactive protein, creatinine, and nitric oxide in cynomolgus macaques. | hantaviruses cause two severe human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). approximately 200,000 cases are reported annually, and there is to date no specific treatment available. a major obstacle in studying the medical aspects of hfrs and hps has been the lack of an adequate animal model. here we show that infection of cynomolgus macaques by wild-type puumala hantavirus resulted in typical signs of hfrs including lethargy, anorexia, prot ... | 2002 | 11739712 |
| a newly discovered variant of a hantavirus in apodemus peninsulae, far eastern russia. | | 2001 | 11747715 |
| first reported case of imported hantavirus pulmonary syndrome in europe. | | 2002 | 11749764 |
| radiographic findings in 20 patients with hantavirus pulmonary syndrome correlated with clinical outcome. | hantavirus is a rare rodent-borne pathogen responsible for the hantavirus pulmonary syndrome. the objective of this study was to review the clinical and radiographic findings of patients presenting with hantavirus pulmonary syndrome in northern alberta, canada. | 2002 | 11756112 |
| [nephropathia epidemica and other hanta virus infections: how rodents' viruses cause disease in human?]. | | 2000 | 11764460 |
| discriminators between hantavirus-infected and -uninfected persons enrolled in a trial of intravenous ribavirin for presumptive hantavirus pulmonary syndrome. | to provide a potentially therapeutic intervention and to collect clinical and laboratory data during an outbreak of hantavirus pulmonary syndrome (hps), 140 patients from the united states with suspected hps were enrolled for investigational intravenous ribavirin treatment. hps was subsequently laboratory confirmed in 30 persons and not confirmed in 105 persons with adequate specimens. patients with hps were significantly more likely than were hantavirus-negative patients to report myalgias from ... | 2002 | 11774075 |
| [microbiological surveillance: viral hemorrhagic fever in central african republic: current serological data in man]. | an investigation was conducted between 1994 and 1997 in forested areas of the central african republic (car) to determine the seroprevalence of igg antibodies against several haemorrhagic fever viruses present in the region. sera were obtained from 1762 individuals in two groups (pygmy and bantu locuted populations) living in 4 forested areas in the south of the country. sera were tested for igg antibodies against ebola, marburg, rift valley fever (rvf), yellow fever (yf) and hantaviruses by enz ... | 2000 | 11775321 |
| serologic survey for hantavirus infections among wild animals in rural areas of são paulo state, brazil. | a serosurvey was conducted in wild animals captured close to two areas where hantavirus cardiopulmonary syndrome (hcps) occurred in são paulo state, brazil. serum samples from a total of 43 mammals were tested for antibodies reactive with sin nombre (sn) hantavirus using a strip immunoblot assay. rnas from the blood clots of the positive samples were submitted to reverse transcriptase-polymerase chain reaction (rt-pcr). two rodents of the genus oligoryzomys were positive for hantavirus antibodie ... | 2001 | 11781602 |
| antibodies anti-chlamydia pneumoniae and anti-mycoplasma pneumoniae in patients with negative serology for hantavirus. retrospective study. | the seroprevalence of chlamydia pneumoniae and mycoplasma pneumoniae in hantavirus seronegative patients, who had symptoms and signs compatible with pneumonia was established. for this purpose we used the indirect fluorescent antibody test. titers > or = 1:16 for c. pneumoniae and m. pneumoniae were found in 8.6% and 17.1% of the serum, respectively, showing evidence of recent or current infection. | 2001 | 11784935 |
| patterns of infection with laguna negra virus in wild populations of calomys laucha in the central paraguayan chaco. | in 1995, an outbreak of hantavirus pulmonary syndrome occurred in the central paraguayan chaco. the primary reservoir of the virus, laguna negra virus, was identified as the vesper mouse, calomys laucha. over a 15-month period, we collected 1,090 small mammals at 12 locations representing 4 habitats common in the central paraguayan chaco. calomys laucha was common in agricultural habitats and uncommon in the native forest habitat. populations of c. laucha were greater during the dry season month ... | 2001 | 11791973 |
| the assessment of human health risks from rodent-borne diseases by means of ecological studies of rodent reservoirs. | zoonoses in general, and more specifically rodent-borne diseases, have proven to be of increasing importance in recent decades. the study of vector biology, therefore, is the foundation for understanding the infection mechanisms for humans. military operations on the european and asian continent have been substantially affected by hantavirus infections during world war i and world war ii, the korean war, and the more recent events in bosnia. the recently discovered hantavirus serotypes with high ... | 2002 | 11799818 |
| [treatment refractory fever... und then also the kidneys fail]. | | 2001 | 11808449 |
| increased glomerular permeability in patients with nephropathia epidemica caused by puumala hantavirus. | nephropathia epidemica (ne) is the scandinavian type of haemorrhagic fever with renal syndrome. usually heavy transient proteinuria is present at the onset of the disease. | 2002 | 11812874 |
| [analysis on the genetics of hantavirus s85-46 isolated from sichuan province]. | to understand the molecular and epidemic characteristics of hantavirus s85-46 isolated from sichuan province. | 2001 | 11851062 |
| characteristics of puumala and dobrava infections in croatia. | in this study, two different hantaviruses, puumala virus (puuv) and dobrava virus (dobv), were demonstrated for the first time to coexist and cause hemorrhagic fever with renal syndrome (hfrs) in croatia. phylogenetic analysis showed some differences among the nucleotide sequences of puuv originating from dinara mountain, which was more closely related to austrian puuv than other croatian puuv from mala kapela mountain. more consistency was found among the croatian dobv. hfrs was verified in 85 ... | 2002 | 11857535 |
| [study on the genetic difference of seo type hantaviruses]. | to understand the genetic type of hantaviruses and the difference between them caused by rodents in beijing and to furhter explore the source of the infectious factors. | 2000 | 11860814 |
| [safety and immunogenicity of inactivated bivalent ehf vaccine in humans]. | safety and immunogenicity of inactivated bivalent ehf vaccine in humans were evaluated in the epidemic area of zhejiang province, china. | 2000 | 11860832 |
| atypical respiratory pathogens. | the main atypical pathogens in respiratory tract infections are classified on the basis of their ability to cause atypical pneumonia. this is not a well-defined clinical entity, and it is evident that atypical pathogens can sometimes cause 'typical' pneumonias and vice versa. this emphasizes the need for microbiological diagnosis, since it affects the selection of proper treatment, in which beta-lactam antibiotics and aminoglycosides are not effective. moreover, mixed infections caused by atypic ... | 1997 | 11864199 |
| haemorrhagic viruses as bioweapons. | | 2001 | 11871792 |
| cdc's strategic plan for bioterrorism preparedness and response. | the department of health and human services (dhhs) has played a critical lead role over the past two years in fostering activities associated with the medical and public health response to bioterrorism. based on a charge from secretary donna shalala in 1998, the centers for disease control and prevention (cdc) is leading public health efforts to strengthen the nation's capacity to detect and respond to a bioterrorist event. as a result of our efforts, federal, state, and local communities are im ... | 2001 | 11880662 |
| the rna binding domain of the hantaan virus n protein maps to a central, conserved region. | the nucleocapsid (n) protein of hantaviruses encapsidates both viral genomic and antigenomic rnas, although only the genomic viral rna (vrna) is packaged into virions. to define the domain within the hantaan virus (htnv) n protein that mediates these interactions, 14 n- and c-terminal deletion constructs were cloned into a bacterial expression vector, expressed, and purified to homogeneity. each protein was examined for its ability to bind the htnv s segment vrna with filter binding and gel elec ... | 2002 | 11884555 |
| transfection-mediated generation of functionally competent tula hantavirus with recombinant s rna segment. | since the discovery of rna recombination in polioviruses, there has been a general belief that this mechanism operates only in positive-sense rna viruses. recently, studying wild-type tula hantavirus, we observed a mosaic-like structure of the s rna segment that was consistent with generation by recombination between viruses from two genetic lineages. here we show transfection-mediated rescue of tula virus carrying recombinant s rna segment. independent attempts yielded s rna molecules of simila ... | 2002 | 11889055 |
| [puumala virus infection (nephropathia epidemica) as different diagnosis of acute renal failure]. | puumala virus infection (nephropathia epidemica) as different diagnosis of acute renal failure. | 2002 | 11894176 |
| serological divergence of dobrava and saaremaa hantaviruses: evidence for two distinct serotypes. | in order to investigate the serological relationship of dobrava hantavirus (dobv, originating from slovenia) and the dobrava-like saaremaa virus (saav, recently discovered in estonia) we analysed 37 human serum samples, 24 from estonia and 13 from the balkans, by focus reduction neutralization test (frnt). most of the estonian sera (19), including all sera from saaremaa island (12), reacted with higher frnt end-point titres to the local saav; the majority of them (15 and 11, respectively), with ... | 2002 | 11895097 |
| analysis of hantavirus genetic diversity in argentina: s segment-derived phylogeny. | nucleotide sequences were determined for the complete s genome segments of the six distinct hantavirus genotypes from argentina and for two cell culture-isolated andes virus strains from chile. phylogenetic analysis indicates that, although divergent from each other, all argentinian hantavirus genotypes group together and form a novel phylogenetic clade with the andes virus. the previously characterized south american hantaviruses laguna negra virus and rio mamore virus make up another clade tha ... | 2002 | 11907216 |
| hantavirus nucleocapsid protein interacts with the fas-mediated apoptosis enhancer daxx. | hantaviruses cause two severe diseases, haemorrhagic fever with renal syndrome in eurasia and hantavirus pulmonary syndrome in the americas. to understand more about the molecular mechanisms that lead to these diseases, the associations of puumala virus nucleocapsid protein (puuv-n) with cellular proteins were studied by yeast two-hybrid screening. daxx, known as an apoptosis enhancer, was identified from a hela cdna library and its interaction with puuv-n was confirmed by gst pull-down assay, c ... | 2002 | 11907324 |
| identification of a cell surface 30 kda protein as a candidate receptor for hantaan virus. | cellular receptors play an important role in virus pathogenesis. as a first step in virus infection, viruses attach to specific receptors on the surface of cells; hantaan virus infects susceptible cells by attaching to a receptor located on the cell surface. to date, the identity of the hantaan virus host cell receptor remains unknown. to determine the protein on the cell surface to which hantaan virus binds, a virus overlay protein-binding assay was performed with radiolabelled virus. a 30 kda ... | 2002 | 11907325 |
| mechanisms of integrin-mediated virus attachment and internalization process. | viruses that propagate within vertebrate hosts have adapted many strategies to infect host cells. one of the first steps in a viral infection is the binding of the virus to cell surface molecules. this interaction between a virus and its receptors plays a key role in the multiplication cycle. entry of viruses into cells is a complex, multistep process, and for several viruses, cell attachment and internalization are distinct steps. a number of virus receptors have been identified; a common famil ... | 2001 | 11922076 |
| establishment of a deer mouse (peromyscus maniculatus rufinus) breeding colony from wild-caught founders: comparison of reproductive performance of wild-caught and laboratory-reared pairs. | the deer mouse (peromyscus maniculatus) is a natural reservoir for several human pathogens, but little is known about the mechanisms by which such pathogens are maintained in nature. as a first step toward developing a colony of deer mice that were permissive for infection with sin nombre (sn) hantavirus, we collected 68 wild p. maniculatus rufinus from central new mexico. mice from this cohort were used to establish 26 breeding pairs, of which 85% were fertile. in subsequent generations, fertil ... | 2001 | 11924788 |
| acute disseminated encephalomyelitis following nephropathia epidemica. | acute disseminated encephalomyelitis (adem) is an acute monophasic inflammatory and demyelinating disease of the central nervous system (cns) occurring days to weeks after a virus infection or vaccination. nephropathia epidemica (ne) is a haemorrhagic fever with renal syndrome caused by puumala virus, with endemic regions in europe, especially scandinavia and western russia. we describe a case of severe nephropathia epidemica requiring dialysis, followed by severe cns symptoms caused by adem. to ... | 2002 | 11939950 |
| hantavirus pulmonary syndrome: the new american hemorrhagic fever. | the recognition of hantavirus pulmonary syndrome (hps) after the investigation of a cluster of unexplained respiratory deaths in the southwestern united states during the spring of 1993 showcased our ability to recognize new and emerging diseases, given the correct juxtaposition of a new clinical entity with circumscribed epidemiologic features that are analyzed with novel diagnostic methods. in less than a decade, hps has become established as a pan-american zoonosis due to numerous viruses mai ... | 2002 | 11941549 |
| herpesvirus hijacks an integrin. | | 2002 | 11944033 |
| mapping of b-cell epitopes in the nucleocapsid protein of puumala hantavirus. | hantavirus nucleocapsid protein (n) has been proven to induce highly protective immune responses in animal models. the knowledge on the mechanisms behind n-induced protection is still limited, although recent data suggest that both cellular and humoral immune responses are of importance. for a detailed b-cell epitope mapping of puumala hantavirus (puuv) n, we used recombinant n derivatives of the russian strain cg18-20 and the swedish strain vranica/hällnäs, as well as overlapping synthetic pept ... | 2002 | 11952140 |
| emerging viral infections. | the past decade has witnessed the emergence of several significant viral pathogens and the further evolution of additional viral pathogens. transmitted by a variety of differing routes, these organisms have presented substantial intellectual challenges to medicine of the 20th and 21st centuries. as perhaps the benchmark pathogen of the past decade, hiv has provided medicine and society with a most formidable opponent, and one that has yet to be fully conquered. nonetheless, a variety of addition ... | 2001 | 11964867 |
| [hantavirus in human and rodent population in an endemic area for hantavirus pulmonary syndrome in argentina]. | this paper analyzed the prevalence and distribution of serological reactivity to hantavirus (antibody against andes virus) of human population exposed to hantavirus and rodents trapped in the studied area. this study was developed in salta (orán and san martín departments), area with the highest incidence for hantavirus pulmonary syndrome (hps) in argentina. in december 1997, 453 healthy people were studied by serology and 39 rodents by serology and pcr. the studied individuals were distributed ... | 2002 | 11965841 |
| andes virus and first case report of bermejo virus causing fatal pulmonary syndrome. | two suspected hantavirus pulmonary syndrome (hps) cases from bolivia occurred in may and july 2000 and were confirmed by enzyme-linked immunosorbent assay (elisa)-andes using n-andes recombinant antigen serology. clot rnas from the two patients were subjected to reverse transcription-polymerase chain reaction (pcr) amplification and sequencing. we describe two characterized cases of hps. one was caused by infection with bermejo virus and the other with andes nort viral lineage, both previously o ... | 2002 | 11971782 |
| emerging infectious diseases in wildlife. | the processes which give rise to emerging infectious diseases of wildlife can be categorised as follows: ecosystem alterations of anthropogenic or natural origin; movement of pathogens or vectors, via human or natural agency; and changes in microbes or in the recognition of emerging pathogens due to advances in the techniques of epidemiology. these are simplistic divisions because factors influencing the emergence of diseases of wild animals generally fall into more than one category. mycoplasmo ... | 2002 | 11974625 |
| hantavirus pulmonary syndrome: at the crossroads. | the initial identification in 1993 of hantavirus pulmonary syndrome as a novel, highly fatal respiratory illness among american indians in the southwestern usa in 1993 opened the window to the recognition of a well-established pan-american zoonosis with a myriad of causative viruses and rodent vectors, although all are new world hantaviruses among new world sigmodontine rodents. the clinical spectrum of symptoms has also been expanded to include asymptomatic infection through to fulminant hemorr ... | 2001 | 11979134 |
| [cloning and nucleotide sequencing of full length l segment cdna of hantavirus strain a9]. | cloning and nucleotide sequencing of full length cdna sequence of l segment of hantavirus strain a9. | 2001 | 11986701 |
| [detection of the expression of alpha3-integrin on hantavirus permissive cells]. | to express and purify human alpha3-integrin to serve as the antigen to prepare its antibody and to separate the vero cell clones without expression of alpha3-integrin. | 2002 | 11986744 |
| [molecular cloning,nucleotides sequence and transient expression of s and m genome segment of hantavirus strain 84fli]. | to sequence, analyze and express the nucleotide sequences of s and m segments of hantavirus strain 84fli. | 2002 | 11986746 |
| [serologic detection of hantavirus antibodies]. | within the framework of diagnosis of highly dangerous infections in the army of the czech republic serological assessment of hantaviral antibodies was introduced. because military units in the field can be in contact with sources of hantaviral infections, the authors investigated this population sample. in 1999-2000 a total of 542 healthy subjects were examined (age 20-40 years). hantaviral antibodies were detected in 9. in three antibodies against the serotype hantaan were found, in two antibod ... | 2002 | 11987584 |
| sequence and expression analysis of deer mouse interferon-gamma, interleukin-10, tumor necrosis factor, and lymphotoxin-alpha. | deer mice (peromyscus maniculatus) are the principal host species of sin nombre (sn) virus, the primary etiologic agent of hantavirus cardiopulmonary syndrome in north america. the disease is a cytokine-mediated immunopathology characterized by pulmonary mononuclear infiltrates without discernible viral pathology. infected deer mice remain life-long carriers and virus is found in many organs, including the lungs, but without pathology. it is unclear how deer mice respond to sn virus because no t ... | 2002 | 11991673 |
| [a new case of hantavirus disease in spain?]. | | 2002 | 11996751 |
| tula hantavirus in belgium. | european common voles (microtus arvalis), captured in belgium in 1999, were proven by molecular as well as by serological techniques to be infected with tula hantavirus (tulv). this is the first evidence for the presence of tulv in this country. no indication of spill-over infections of puumala virus, known to be highly endemic among bank voles (clethrionomys glareolus) within the same geographical regions as the trapped tulv-infected common voles, was observed. together with previous reports on ... | 2002 | 12002543 |
| hantavirus nucleocapsid protein coiled-coil domains. | the nucleocapsid (n) proteins of hantaviruses such as the sin nombre virus (snv) bind to membranes and viral rnas, associate with transcription and replication complexes, and oligomerize during the process of virus assembly. n proteins trimerize in vitro and in vivo, and associate via assembly domains at their amino- and carboxyl-terminal ends. because structure prediction algorithms suggested that n protein residues 3-75 form two coiled-coil motifs separated by an intervening kink or turn seque ... | 2002 | 12019266 |
| hantaviruses: an overview. | hantaviruses are a newly emerging group of rodent-borne viruses that have significant zoonotic potential. human infection by hantaviruses can result in profound morbidity and mortality, with death rates as high as 50%, and potentially long-term cardiovascular consequences. hantaviruses are carried by peridomestic and wild rodents worldwide and have occasionally been linked to infections in laboratory rodents. because these viruses have been associated with significant human disease, they have be ... | 2002 | 12022401 |
| hantavirus infection with marked sinus bradycardia, taiwan. | | 2002 | 12023927 |
| double trouble: hemorrhagic fever with renal syndrome and leptospirosis. | the clinical picture of hemorrhagic fever with renal syndrome can closely mimic that of unicteric leptospirosis and vice versa. this is the first description of dual infection with dobrava virus and leptospira and alteration of immune parameters in a croatian soldier. | 2002 | 12030403 |
| assessment of ecologic and biologic factors leading to hantavirus pulmonary syndrome, colorado, u.s.a. | to understand the ecologic parameters of sin nombre virus (snv; family bunyaviridae, genus hantavirus) infections in the deer mouse (peromyscus maniculatus), environmental variables impacting the rodent populations, and the conditions under which snv is amplified. this may help us understand the antecedents of human risk for developing hantavirus pulmonary syndrome (hps) as a consequence of snv infection. | 2002 | 12035141 |
| hunting down hantavirus. | | 2002 | 12035655 |
| genetics of hantaviruses: implications to taxonomy. | hantaviruses (genus hantavirus, family bunyaviridae) represent a prime example of emerging viruses. since isolation of the prototype hantaan virus in the late 70s more than 20 new species have been described and the number is increasing fast thus demanding for a more refined classification. taking into account that hantaviruses are difficult to isolate in cell culture, one should not be surprised that most of the "newcomers" were first described as distinct hantavirus genotypes. moreover, the on ... | 2002 | 12038679 |
| serological evidence of hantavirus infections in malaysia. | hantaviruses are primarily rodent-borne and transmission is by inhalation of virus-contaminated aerosols of rodent excreta, especially urine and saliva. the genus hantavirus, family bunyaviridae, comprises at least 14 serotypes and the symptoms of clinical illness range from mild fever to severe hemorrhagic manifestations with renal complications. many countries in southeast asia are unaware of the importance of hantavirus infections and give them low priority. malaysia, like other countries in ... | 2001 | 12041558 |
| [seroprevalence of antibodies against hantavirus in 10 communities of the ix region of chile where hantavirus infection were diagnosed]. | thirty six cases of clinical hantavirus cardiopulmonary syndrome occurred in the ix region of chile. most of these patients were young males, farm or timber workers, who lived near the andes mountain chain. | 2002 | 12043366 |
| identification of tula hantavirus in pitymys subterraneus captured in the cacak region of serbia-yugoslavia. | atypical serum neutralizing antibody responses to prototype strains of puumala viruses in some patients with hemorrhagic fever with renal syndrome (hfrs) have long suggested the existence of other hantaviruses in the balkans. | 2002 | 12044299 |
| cross-protection against challenge with puumala virus after immunization with nucleocapsid proteins from different hantaviruses. | hantaviruses are rodent-borne agents that cause hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome in humans. the nucleocapsid protein (n) is relatively conserved among hantaviruses and highly immunogenic in both laboratory animals and humans, and it has been shown to induce efficient protective immunity in animal models. to investigate the ability of recombinant n (rn) from different hantaviruses to elicit cross-protection, we immunized bank voles with rn from puumala (puuv) ... | 2002 | 12050380 |
| hantavirus-specific antibodies in rodents and humans living in kuwait. | hantaviruses are found in widely scattered areas of the world and are transmitted by inhalation of virus-contaminated aerosols of rodent excreta. the present study was undertaken in kuwait to investigate the serological evidence for hantavirus infection in rodents and humans. sera were collected from 283 wild rodents and 183 human subjects (46 kuwaitis and 137 non-kuwaitis). the rodent sera were investigated for the presence of antibodies against the seoul and puumala strains of the hantaviruses ... | 2002 | 12052569 |
| acute renal failure caused by leptospirosis and hantavirus infection in an urban hospital. | background: leptospirosis (ls) and hantavirus (hv) infection have many common clinical manifestations, including acute renal failure. in as much as clinical experience with these diseases is quite limited in urban centers, we identified cases of leptospira and hv-induced acute renal failure and compared the clinical course and evolution of these diseases. methods: patients hospitalized with acute renal failure, suspected to be caused by infection, were retrospectively studied over a 13-year peri ... | 2002 | 12067823 |
| tula virus infection associated with fever and exanthema after a wild rodent bite. | reported here is the first case of human acute infection with tula virus, which occurred in a 12-year-old boy in switzerland. this hantavirus had been considered apathogenic to humans, and in switzerland only tulv-genome sequences have been demonstrated in wild rodents to date. in this case, paronychia, fever and exanthema occurred after the patient was bitten by a wild rodent, indicating an unusual route of hantavirus transmission. thus, tula virus infection should be taken into account in pati ... | 2002 | 12072943 |
| viral agents as biological weapons and agents of bioterrorism. | multiple viral agents have been classified by the cdc as potential weapons of mass destruction or agents for biologic terrorism. agents such as smallpox, viral hemorrhagic fever viruses, agents of viral encephalitis, and others are of concern because they are highly infectious and relatively easy to produce. although dispersion might be difficult, the risk is magnified by the fact that large populations are susceptible to these agents and only limited treatment and vaccination strategies exist. ... | 2002 | 12074486 |
| genetic vaccines protect against sin nombre hantavirus challenge in the deer mouse (peromyscus maniculatus). | we used a deer mouse (peromyscus maniculatus) infection model to test the protective efficacy of genetic vaccine candidates for sin nombre (sn) virus that were known to provoke immunological responses in balb/c mice (bharadwaj et al., vaccine 17, 2836-2843, 1999 ). protective epitopes were localized in each of four overlapping cdna fragments that encoded portions of the sn virus g1 glycoprotein antigen; the nucleocapsid gene also was protective. the protective efficacy of glycoprotein gene fragm ... | 2002 | 12075094 |