Publications

antigenic properties and diagnostic potential of recombinant dobrava virus nucleocapsid protein.	dobrava hantavirus (dobv) causes severe hemorrhagic fever with renal syndrome in the balkan region and has been detected recently also in russia, estonia, and germany. dobv nucleocapsid protein (n) was produced in insect cells, using the baculovirus expression system (bac-dobv-n), and in e. coli as a truncated (aa 1-165) glutathione-s transferase fusion protein (dobv-dn-gst). the antigenic properties of bac-dobv-n were found identical to native dobv-n when examined by a panel of hantavirus-speci ...	2000	10797384
[hantavirus infections].		2000	10943219
seroepidemiology of hantavirus in the philippines.	this study was undertaken to determine the seroepidemiology of hantavirus infection in the philippines.	2000	10737848
rodent-borne emerging viral zoonosis. hemorrhagic fevers and hantavirus infections in south america.	hantaviruses and arenaviruses are naturally occurring viruses of rodents. four south american hemorrhagic fevers caused by arenaviruses have emerged in the last 5 decades. all have similar clinical manifestations, with a case-fatality rate as high as 15% to 30%. hantavirus infections have been increasingly recognized in south america since the description in 1993 of hantavirus pulmonary syndrome. given the diversity of rodent species in the region, it can be foreseen that many other viruses will ...	2000	10738678
hantavirus pulmonary syndrome--panama, 1999-2000.	hantavirus pulmonary syndrome (hps) is an acute viral rodentborne zoonosis characterized by severe cardiopulmonary illness with a 40%-60% case-fatality rate. since its identification in the united states in 1993, the recognized clinical spectrum of illnesses associated with human hantavirus infection has expanded to include mild illness, and case-patients have been identified in canada and south america. this report describes the first confirmed hps cases from central america and summarizes prel ...	2000	10738839
[simultaneous leptospirosis and hantavirus infection in the same patient].	the leptospirosis and the hantaviral infections are worldwide distributed zoonoses having the similar epidemiology and clinical symptoms. both in europe and asia those hantaviral serotypes are common which are responsible for the haemorrhagic fever with renal syndrome, while on the american continent the hantaviral pulmonary syndrome has also been diagnosed. authors describe a patient who had simultaneous leptospira and hantaviral infections with haemorrhagic fever as well as with mild, transien ...	2000	10750403
serologic evidence of puumala virus infection in wild moose in northern sweden.	puumala (puu) virus is the causative agent of nephropathia epidemica, the scandinavian form of hemorrhagic fever with renal syndrome. the infection is acquired by airborne transmission of puu virus from its rodent reservoir, the bank vole. besides serologic data indicating that the virus may spread also to heterologous rodents, there is little information on the susceptibility of wild living animals to puu virus. we studied the occurrence of antibodies to puu virus in serum samples from 427 wild ...	2000	10761733
[serologic study of the prevalence and course of hantavirus infections in mecklenburg-vorpommern].	694 sera obtained from 1994 till 1998 in the general population were examined with a recombinant enzyme immunoassay for hantavirus antibodies against the serotypes hantaan and puumala. positive samples were retested for confirmation with an indirect immunofluorescence assay and a recombinant immunoblot. the observed antibody prevalence of 0.9% signities that mecklenburg-vorpommern is not an endemic area. 12 out of 196 patients with a suspected hantavirus infection were confirmed. all proved anti ...	2000	10740354
development and evaluation of a solid-phase enzyme immunoassay based on andes hantavirus recombinant nucleoprotein.	hantavirus pulmonary syndrome (hps) with high mortality rate has been reported in five countries in south america. rapid accurate methods are important both for monitoring acute infections and for epidemiological studies. the andes virus nucleoprotein amino acid sequence has a high identity percentage compared with other sequences of this region and has been chosen for the development of diagnostic reagents. andes nucleoprotein expressed in escherichia coli was applied as antigen in igg, iga and ...	2000	10670565
hantavirus pulmonary syndrome in brazil: clinical aspects of three new cases.	hantavirus pulmonary syndrome (hps) has been recognized recently in brazil, where 28 cases have been reported as of september 1999. we report here the clinical and laboratory findings of three cases whose diagnoses were confirmed serologically. all the patients were adults who presented a febrile illness with respiratory symptoms that progressed to respiratory failure that required artificial ventilation in two of them. laboratory findings were most of the time consistent with those reported in ...	2000	10742726
pulmonary haemorrhage as a predominant cause of death in leptospirosis in seychelles.	we examined the cause of death during a 12-month period (1995/96) in all consecutive patients admitted to hospital with leptospiral infection in seychelles (indian ocean), where the disease is endemic. leptospirosis was diagnosed by use of the microscopic agglutination test and a specific polymerase chain reaction assay on serum samples. seventy-five cases were diagnosed and 6 patients died, a case fatality of 8%. all 6 patients died within 9 days of onset of symptoms and within 2 days of admiss ...	2000	10748905
sin nombre hantavirus disease in a ten-year-old boy and his mother.		2000	10749469
sin nombre virus (snv) ig isotype antibody response during acute and convalescent phases of hantavirus pulmonary syndrome.	serum samples from 22 hantavirus pulmonary syndrome (hps) patients were tested for sin nombre virus (snv)-reactive antibodies. in the acute phase of hps, 100% and 67% of the samples tested positive for snv-specific immunoglobulin (ig) m and iga, respectively. among the virus-specific igg antibodies, the most prevalent were igg3 (in 97% of samples), followed by igg1 (70%), igg2 (30%), and igg4 (3%).	2000	10756154
[clinical case of the month. a new case of nephropathia epidemica due to puumala virus].	nephropathia epidemica due to puumala virus has already been described in belgium. its evolution is usually favourable. the main symptoms are fever, a decrease platelet count, proteinuria and acute renal insufficiency, which, in an epidemiological context, quickly point the correct diagnosis. acute and transitory myopia is typical. nevertheless, the clinical presentation, including multisystemic symptoms may lead to the false diagnosis of an acute bacterial or immune disease.	2000	10769579
annual update of systemic disease--1999: emerging and re-emerging infections (part ii).		2000	10770510
spatial and temporal dynamics of puumala hantavirus infection in red bank vole (clethrionomys glareolus) populations in belgium.	dynamics of hantavirus infection and population densities in rodents were investigated from 1996 to 1999 in southern belgium. evidence of puumala infection was restricted to clethrionomys glareolus. although the serotype was not determined, antibodies against hantavirus were also found in eight apodemus sylvaticus. in fall 1996, the seroprevalence in c. glareolus was high (20.1%, 37 of 184) and the infection was widely distributed in the area studied whereas a focal occurrence of positive rodent ...	2000	10773322
epidemiology of community-acquired pneumonia in adults: a population-based study.	in this prospective study, the authors assessed the incidence, aetiology, and outcome of patients with community-acquired pneumonia in the general population. from december 1993 to november 1995, a study was performed in a mixed residential-industrial urban population of the "maresme" region in barcelona, spain. all subjects > or =14 yrs of age (annual average population size 74,368 inhabitants) with clinically suspected community-acquired pneumonia were registered. all cases were re-evaluated b ...	2000	10780770
renal failure and hantavirus infection in europe.		2000	10831620
diagnostic potential of puumala virus nucleocapsid protein expressed in drosophila melanogaster cells.	puumala virus (puu) nucleocapsid protein (n) was expressed in insect cells by using the drosophila expression system (des; invitrogen bv, groningen, the netherlands). stable transfectants were established by hygromycin b selection and showed continuous expression of the recombinant protein (des-puu-n) for at least 5 months. the antigenic property of des-puu-n was shown to be identical to that of native puu n when examined with a panel of hantavirus-specific monoclonal antibodies. enzyme-linked i ...	2000	10834996
renal function and blood pressure five years after puumala virus-induced nephropathy.	nephropathia epidemica (ne) is a mild form of hemorrhagic fever with renal syndrome caused by puumala hantavirus. its long-term prognosis is considered favorable. some reports suggest, however, that a previous hantavirus infection increases the risk of hypertension.	2000	11012905
pronounced monocytosis in a case of nephropathia epidemica.	nephropathia epidemica is a mild form of haemorrhagic fever with renal syndrome. thrombocytopenia is common and characteristic. we report here a case of a young man with nephropathia epidemica and marked transient monocytosis.	2000	10959653
evolutionary diversification of protein-coding genes of hantaviruses.	phylogenetic analyses of the s:, m, and l: genes of the hantaviruses (bunyaviridae: hantavirus) revealed three well-differentiated clades corresponding to viruses parasitic on three subfamilies (murinae, arvicolinae, and sigmodontinae) of the rodent family muridae. in rooted trees of m: and l: genes, the viruses with hosts belonging to murinae formed an outgroup to those with hosts in arvicolinae and sigmodontinae. this phylogeny corresponded with a phylogeny of the murid subfamilies based on mi ...	2000	11018161
setting a trap for hantavirus.		2000	11022538
[serological diagnosis and nasopharyngeal washings in pediatric infections].	in the course of respiratory infections, the efficacy of microbiologic diagnosis has increased years after years, in term of specificity, sensitivity and rapidity. new pathogenic agents have been described such as: legionella pneumophila, chlamydia pneumoniae, hantavirus. some viruses have been well characterized as responsible for seasonal outbreaks using rapid tools for identification. needs for efficient diagnostic tools became more obvious when specific antiviral drugs appeared on the market ...	2000	11028183
[hantavirus: recent data and review of the literature].	hantavirus (bunyaviridae family) are present worldwide and are the causative agent of haemorrhagic fever with renal or pulmonary syndrome. these viruses are transmitted by rodents with asymptomatic infection which thus act as an excellent reservoir. molecular epidemiology indicates that hantavirus may have evolved in three ways: mutations within the genome, reassortment of the segmented genome between two closely related hantavirus, and genomic recombination, a relatively rare phenomenon among n ...	2000	11030053
hantavirus pulmonary syndrome is distinguishable from acute interstitial pneumonia.	hantavirus pulmonary syndrome (hps) and acute interstitial pneumonia (aip) can share similar clinical presentations. aip is an acute, diffuse lung disease that has some clinical features suggesting a viral infection, although causative agent(s) have not been identified.	2000	11035576
lack of evidence for an association between hantavirus infections and wegener's granulomatosis, microscopic polyangiitis, churg-strauss syndrome and giant cell arteritis.		2000	11136889
[hantavirus disease during pregnancy].	hantavirus infection is known to occur in mice and rats without symptoms. in contrast, an infection with hantavirus in humans gives influenzalike symptoms and acute renal failure. the treatment is symptomatic, but treatment with dialysis can be necessary for a while. a case of hantavirus infection in denmark in a 30 week pregnant woman is reported.	2000	11043050
hantavirus pulmonary syndrome in northern alberta, canada: clinical and laboratory findings for 19 cases.	we reviewed the clinical and laboratory findings for 19 cases of hantavirus pulmonary syndrome (hps) identified either serologically or by immunohistochemical testing of archival tissue at our tertiary care center. fever (95%), cough (89%), and dyspnea (89%) were the most common presenting symptoms. the most prevalent presenting signs were respiratory abnormalities (95%) and tachycardia (84%). common laboratory findings included thrombocytopenia (95%) and leukocytosis (79%). elevated aspartate a ...	2000	11049774
expression of a human, neutralizing monoclonal antibody specific to puumala virus g2-protein in stably-transformed insect cells.	we cloned the heavy- and light-chain antibody genes of a human x (humanxmouse) trioma secreting a neutralizing, igg monoclonal antibody to the g2-protein of puumala virus. the antibody genes were inserted separately into plasmid transfer vector piei-4 such that the genes were under control of the baculovirus immediate early gene promoter, iei. trichoplusia ni (tn) cells were co-transfected with these constructs and a selection plasmid containing a neomycin-resistance gene. cloned transformants e ...	2000	11121551
[zoonoses among immigrants].	during the last ten years denmark has received an increasing number of immigrants, especially from the balkans, the middle east, and somalia. some of these may suffer from the zoonoses occurring in their country of origin. but apart from echinococcosis, zoonoses in these immigrants do not seem to pose a quantitatively greater problem than in danes who have visited these areas. however it is important to have a knowledge of the symptoms and mode of transmission of zoonoses occurring in areas wher ...	2000	11107966
serological study of hantavirus in the rodent population of nakhon pathom and nakhon ratchasima provinces thailand.	a serological survey has been carried out to detect evidence of hantavirus infection in rodents from two provinces of thailand. this study aimed to examine virus antibody in 354 rodents trapped among 6 different villages of nakhon pathom province (february-march, 1998) and in 326 rodents trapped among 14 villages of nakhon ratchasima province (august-october, 1998). seroprevalence among rodents from nakhon pathom province (2.3%), was mostly find in rattus norvegicus (3.8%) and bandicota indica ( ...	2000	11127326
in vitro antiviral activity of lactoferrin and ribavirin upon hantavirus.	bovine lactoferrin (lf) and ribavirin (rbv) were tested as antiviral agents against seoul type hantavirus (sr-11 strain) in vitro. hantaviral foci number in vero e6 cells infected with sr-11 was reduced with lf treatment by 5 days post infection to obtain a 50% effective dose (ed50) of 2500 microg/ml, while pretreatment with lf was highly efficacious having an ed50 of 39 microg/ml. conversely, 1 h pretreatment with rbv revealed no inhibition of viral focus formation but could significantly reduc ...	2000	11003470
genetic analysis of wild-type dobrava hantavirus in slovenia: co-existence of two distinct genetic lineages within the same natural focus.	genetic analysis was performed of wild-type (wt) dobrava hantavirus (dob) strains from slovenia, the country where the virus was first discovered and where it was found to cause haemorrhagic fever with renal syndrome (hfrs), with a fatality rate of 12%. two hundred and sixty mice of the genus apodemus:, trapped in five natural foci of dob-associated hfrs during 1990-1996, were screened for the presence of anti-hantavirus antibodies and 49 apodemus: flavicollis and four apodemus: agrarius were fo ...	2000	10859380
hantavirus infections among mammalogists studied by focus reduction neutralisation test.		2000	11117649
puumala (puu) hantavirus strain differences and insertion positions in the hepatitis b virus core antigen influence b-cell immunogenicity and protective potential of core-derived particles.	hepatitis b virus (hbv) core-derived chimeric particles carrying a puumala (puu) hantavirus (strain vranica/hällnäs) nucleocapsid (n) protein sequence (aa 1-45), alternatively inserted at three distinct positions (n-, c-terminus, or the internal region), and mosaic particles consisting of hbv core as well as core/puu (vranica/hällnäs) n (aa 1-45) readthrough protein were generated. chimeric particles carrying the insert at the n-terminus or the internal region of core induced some protective imm ...	2000	11040127
[epidemiologic problems in hantavirus infections].	the objective of the submitted paper was to present some information pertaining to epidemiological problems of hantavirus infection. in the czech republic the prevalence of these infections is not alarming but in the world cases of haemorrhagic fever with renal syndrome (hfrs) as well as hantavirus pulmonary syndrome (hps) are reported. on the territory of the czech republic there are foci of hantaviruses and in conjunction with summer tourism or working activities abroad the import of this infe ...	2000	10802945
detection of hantaan and seoul viruses by reverse transcriptase-polymerase chain reaction (rt-pcr) and restriction fragment length polymorphism (rflp) in renal syndrome patients with hemorrhagic fever.	hemorrhagic fever with renal syndrome (hfrs), also called korean hemorrhagic fever (khf), is the most common cause of acute renal failure in the far east. two serotypes of hantavirus, hantaan and seoul viruses are known pathogens for hfrs in korea.	2000	10711409
scientific community. controversy claims cdc lab chief.		2000	10712145
clinical aspects of nephropathia epidemica (puumala virus infection) in europe: a review.	nephropathia epidemica (ne) is a prevalent zoonosis throughout europe and is caused by the puumala type of hantavirus. the incidence of ne varies in a cyclic fashion, with peaks occurring every 3rd to 4th year, coinciding with peaks in vole populations. the clinical course of ne is generally milder than haemorrhagic fever with renal syndrome caused by hantaviruses in other parts of the world. typically, ne has a sudden onset with fever, headache, backpain and gastrointestinal symptoms. however, ...	2000	10826895
using remotely sensed data to identify areas at risk for hantavirus pulmonary syndrome.	the 1993 u.s. hantavirus pulmonary syndrome (hps) outbreak was attributed to environmental conditions and increased rodent populations caused by unusual weather in 1991- 92. in a case-control study to test this hypothesis, we estimated precipitation at 28 hps and 170 control sites during the springs of 1992 and 1993 and compared it with precipitation during the previous 6 years by using rainfall patterns at 196 weather stations. we also used elevation data and landsat thematic mapper satellite i ...	2000	10827113
remote sensing and geographic information systems: charting sin nombre virus infections in deer mice.	we tested environmental data from remote sensing and geographic information system maps as indicators of sin nombre virus (snv) infections in deer mouse (peromyscus maniculatus) populations in the walker river basin, nevada and california. we determined by serologic testing the presence of snv infections in deer mice from 144 field sites. we used remote sensing and geographic information systems data to characterize the vegetation type and density, elevation, slope, and hydrologic features of ea ...	2000	10827114
[acute renal failure in hantavirus infections].	background: the acute renal failure remains a diagnostic challenge for the clinician. case reports: between 1991 and 1996, acute renal failure caused by hantavirus infection was diagnosed in 4 previously healthy male patients. main symptoms consisted of fever, headache, arthralgia, lumbar and abdominal pain as well as a decline in diuresis. the ultrasonography showed a slight splenomegaly in 2 patients. the clinical chemistry showed elevated serum creatinine from 2.2 mg/dl to 6.7 mg/dl and throm ...	2000	10808304
hantavirus pulmonary syndrome in canada, 1989-1999.		2000	10851764
hantavirus infection in taiwan: the experience of a geographically unique area.	hantaviruses are rodent-borne viruses, and they, mainly the hantaan (htn) serotype, are the causative agents of a group of febrile nephropathies known as "hemorrhagic fever with renal syndrome (hfrs). " despite the fact that hfrs is frequently reported in china, with an annual incidence of 50,000-100,000 cases, one puzzling observation that no local case of hfrs has been confirmed in taiwan has yet to be explained. we hypothesized that the hantavirus strain prevailing in taiwan mainly belongs to ...	2000	10596027
of mice, men, and microbes-hantavirus		2000	10613808
persistent hantavirus infections: characteristics and mechanisms.	hantaviruses include serious human pathogens that are maintained in nature in persistently infected rodents and that can also persistently infect cultured mammalian cells, causing little or no cytopathology. the mechanisms of hantavirus persistence are only beginning to be explored. recent data point to subtle changes in the viral genome that might result in the differential regulation of replication and lead to persistence.	2000	10664598
role of maternal antibody in natural infection of peromyscus maniculatus with sin nombre virus.	data from naturally infected deer mice (peromyscus maniculatus) were used to investigate vertical transmission of sin nombre virus (snv) and snv-specific antibody. the antibody prevalence in juvenile mice (14 g or less) was inversely proportional to the mass of the animal, with juvenile deer mice weighing less than 11 g most likely to be antibody positive (26.9%) and juvenile mice weighing between 13 and 14 g least likely to be antibody positive (12.9%). although a significant sex bias in seropo ...	2000	10666274
from the centers for disease control and prevention. hantavirus pulmonary syndrome--panama, 1999-2000.		2000	10807372
accumulation of terminally deleted rnas may play a role in seoul virus persistence.	two independent, long-term infections were analyzed to determine whether changes in viral replication could contribute to the establishment and/or maintenance of persistent seoul virus infections. infected cell cultures initially contained high levels of infectious virus and intracellular viral rna that peaked between approximately 7 to 16 days postinfection and then gradually declined until day 26. after day 26, the viral titers and the levels of the small (s), medium (m), and large (l) viral r ...	2000	10627543
a neutralizing recombinant human antibody fab fragment against puumala hantavirus.	a combinatorial human antibody fab pcomb3h library, generated from splenic lymphocytes of a puumala hantavirus (puuv) immune individual, was selected against puuv using the phage display technique. panning was carried out with antigens immobilized by mabs directed to the two puuv envelope glycoproteins g1 and g2. thirteen fabs, with reactivity directed to puuv and specifically the g2 protein, as assessed by immunofluorescence and elisa respectively, were isolated in crude preparations. by a focu ...	2000	10686029
transmission of black creek canal virus between cotton rats.	black creek canal (bcc) virus is a hantavirus associated with hantavirus pulmonary syndrome in southeastern north america. the virus was isolated from the spleen of a cotton rat (sigmodon hispidus) trapped in southern florida. our previous studies have shown that we could consistently infect male cotton rats with bcc virus in the laboratory. these animals became persistently infected and virus could be detected in salivary glands, urine, and feces. in this report we show: (1) female and male cot ...	2000	10568766
hantavirus nucleocapsid protein oligomerization.	hantaviruses are enveloped, negative-strand rna viruses which can be lethal to humans, causing either a hemorrhagic fever with renal syndrome or a hantaviral pulmonary syndrome. the viral genomes consist of three rna segments: the l segment encodes the viral polymerase, the m segment encodes the viral surface glycoproteins g1 and g2, and the s segment encodes the nucleocapsid (n) protein. the n protein is a 420- to 430-residue, 50-kda protein which appears to direct hantavirus assembly, although ...	2001	11160704
dobrava hantavirus causes hemorrhagic fever with renal syndrome in central europe and is carried by two different apodemus mice species.	in central europe, hemorrhagic fevers with renal syndrome (hfrs) in humans are caused by the hantavirus species puumala (transmitted by voles) and a second, hantaan-related species (transmitted by mice). the second virus could be identified as dobrava virus. to date, 19 clinical cases of dobrava infection have been found in germany and slovakia. all patients exhibited a mild/moderate clinical course and no case fatality occurred. screening for infected rodents revealed that the striped field mou ...	2001	11170053
dna vaccination of mice with a plasmid encoding puumala hantavirus nucleocapsid protein mimics the b-cell response induced by virus infection.	inoculation of naked dna has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. to study the humoral immune response induced by dna vaccination we cloned the entire nucleocapsid protein-encoding sequence of the puumala hantavirus strain vranica/hällnäs into the cmv promoter-driven expression unit of the plasmid pcdna3, generating pcdna3-vr1. a single dose injection of 50 microg of plasmid dna into each m. tibialis anterior of balb/c mice ...	2001	11035190
hantavirus nucleocapsid protein is expressed as a membrane-associated protein in the perinuclear region.	black creek canal virus (bccv) is a new world hantavirus which is associated with hantavirus pulmonary syndrome. we have examined the site of expression of the bccv nucleocapsid protein (nbccv) in the absence of bccv glycoproteins and found that the majority of the protein is localized to the golgi region. immunofluorescence analysis of bhk21 cells expressing the nbccv and la crosse virus nucleocapsid protein (nlacv) showed different intracellular localization patterns of these proteins within t ...	2001	11160679
hantavirus pulmonary syndrome--vermont, 2000.	in 1993, an outbreak of an unexplained pulmonary illness occurred in the southwestern united states. this outbreak led to the first description of hantavirus pulmonary syndrome (hps), a rodentborne hantaviral infection. hantaviruses have been found in rodents in rural areas throughout the united states, but most infection has occurred in the southwest. this report describes the first hps case in vermont and underscores the importance of preventing exposure to peridomestic rodents and recognizing ...	2001	11476530
infection with sin nombre hantavirus: clinical presentation and outcome in children and adolescents.	sin nombre hantavirus (snv) is the leading causative agent of hantavirus cardiopulmonary syndrome (hcps) in the united states and canada. relatively few cases of hcps have involved children. this report describes the clinical characteristics of a series of pediatric cases of snv infection in the united states and canada from 1993 through march 2000.	2001	11483837
molecular cloning and high-level expression of g2 protein of hantaan (htn) virus 76-118 strain in the yeast pichia pastoris km71.	hantaan viral g2 envelope gene, which is known to be one of major antigens and induce neutralizing antibodies, was cloned into expression vector phil-s1 which consists of aox1 promoter, pho1 signal sequence, his4 gene and other components. the recombined plasmid was transformed into methylotropic yeast, pichia pastoris of km71 and recombinant strains harboring multi-copy of g2 gene were selected. expression of the cloned g2 gene was confirmed with western blot analysis using anti-sera of guinea ...	2001	11324753
highly sensitive taqman pcr detection of puumala hantavirus.	an increasing number of clinical cases of hantavirus infections have been reported from various regions in asia, europe and north america. hantaviruses (family bunyaviridae, genus hantavirus) are enveloped and possess a single-stranded trisegmented rna genome of negative polarity. rodents or insectivores are natural hosts of hantaviruses and transmit the virus to humans chiefly by aerosolisation. these viruses are the causative agents of haemorrhagic fever with renal and pulmonary syndromes. in ...	2001	11489422
[pathogenesis of hemorrhagic fever].		2001	11496775
[hantavirus-related hemorrhagic fever in europe].		2001	11496776
a lethal disease model for hantavirus pulmonary syndrome.	hantaviruses are associated with two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). development of vaccines and therapies to prevent and treat hfrs and hps have been hampered by the absence of a practical animal model. here we report that andes virus (andv), a south american hantavirus, is highly lethal in adult syrian hamsters. the characteristics of the disease in hamsters, including the incubation period, symptoms of rapidly progressing r ...	2001	11601912
physicians' database searches as a tool for early detection of epidemics.	we analyzed retrospectively the use of physician desk reference database searches to identify epidemics of tularemia, nephropathy, pogosta disease, and lyme disease and compared the searches with mandatory laboratory reports to the national infectious diseases register in finland during 1995. continuous recording of such searches may be a tool for early detection of epidemics.	2001	11384534
high prevalence of sin nombre virus in rodent populations, central utah: a consequence of human disturbance?		2001	11384538
hantavirus pulmonary syndrome due to andes virus in temuco, chile: clinical experience with 16 adults.	to describe the clinical features and laboratory abnormalities of 16 adults with confirmed hantavirus pulmonary syndrome (hps) due to andes virus in temuco, chile.	2001	11502657
hantavirus seroconversion of wild-caught peromyscus during quarantine.		2001	11384539
hantavirus pulmonary syndrome in the united states.	since the first outbreak of hantavirus pulmonary syndrome (hps) in 1993, understanding of the vast distribution and potential impact of hantaviruses has grown. at least 277 cases of hps have been documented in the united states. the full clinical spectrum has yet to be elucidated, and one outbreak suggested the possibility of person-to-person transmission. new research has identified the b-3 integrins as cellular receptors for hantaviruses and has determined the pivotal role of the immune system ...	2001	11384556
clinical characterization of dobrava hantavirus infections in germany.	there is increasing evidence that dobrava (dobv) but not hantaan (htnv) hantavirus is a hemorrhagic fever with renal syndrome (hfrs) causing agent in central europe. however, only single clinical cases of hfrs due to acute dobv infection have been described so far. we report on three male patients from a non-endemic hantavirus focus in northern germany who suffered from mild to moderate hfrs strongly resembling nephropathia epidemica. serotyping by detection of hantavirus species-specific neutra ...	2001	11393382
[regional differences and hantavirus pulmonary syndrome].	factors related to the characteristics of hantavirus pulmonary syndrome in argentina are described. factors from different scientific fields converge to form the syndrome's analytical framework. some of these factors are the history of spatial occupation, work and production structures, human migration patterns, ethnic composition, reservoir dynamics and its relationship to the different circulating viruses, and human behavior. furthermore, the multiple factors are expressed in three ecological ...	2001	11426265
molecular epidemiology of viral pathogens and tracing of transmission routes: hepatitis-, calici- and hantaviruses.	background: the need to rapidly identify new therapeutic drugs and vaccines for clinically important viral infections has resulted in intensive study of the molecular properties of viruses. modern molecular techniques have provided tools for tracing infections and studying the evolution of viruses. objective study and design: two examples illustrating how modern molecular techniques can be used in clinical virology and molecular epidemiology (hepatitis and caliciviruses), and one example documen ...	2001	11397654
polymorphism of the cytokine genes in hospitalized patients with puumala hantavirus infection.	nephropathia epidemica (ne) is a mild type of haemorrhagic fever with renal syndrome caused by puumala (puu) hantavirus. the clinical course of ne varies from asymptomatic to fatal. the aim of this study was to establish whether polymorphisms in the cytokine genes are associated with susceptibility to and outcome of ne.	2001	11427627
[complete genome sequence analysis of the hantavirus z10 strain].	study on the complete genome sequence of hantavirus z10 strain which has been applied for inactivated vaccine production in china, to assess its molecular characteristics and the diversity with other hantaviruses.	2001	11436636
hbv core particles as a carrier for b cell/t cell epitopes.	in the middle 80s, recombinant hepatitis b virus cores (hbc) gave onset to icosahedral virus-like particles (vlps) as a basic class of non-infectious carriers of foreign immunological epitopes. the recombinant hbc particles were used to display immunodominant epitopes of hepatitis b, c, and e virus, human rhinovirus, papillomavirus, hantavirus, and influenza virus, human and simian immunodeficiency virus, bovine and feline leukemia virus, foot-and-mouth disease virus, murine cytomegalovirus and ...	2001	11509871
antibody to sin nombre virus in rodents associated with peridomestic habitats in west central montana.	most human cases of hantavirus pulmonary syndrome are acquired in the peridomestic environment, yet studies of the ecology and infection dynamics in the reservoir host, the deer mouse (peromyscus maniculatus), have focused on sylvan populations. we describe a 2.5-year study of hantavirus infection in rodents associated with peridomestic habitats in west central montana. antibodies reactive with sin nombre virus (snv) were found in five species. overall snv antibody prevalence was highest among d ...	2001	11442208
genetic and serotypic characterization of sin nombre-like viruses in canadian peromyscus maniculatus mice.	in canada, hantavirus infected deer mice (peromyscus maniculatus) have been collected from british columbia to newfoundland. partial sequencing of g1 and n protein encoding regions from canadian peromyscus maniculatus-borne hantaviruses demonstrated the existence of significant genotypic divergence among strains. phylogenetic analysis showed that sin nombre (sn)-like viruses from eastern and western canadian deer mice can be divided into at least two broad-based genogroups. sequencing of mitocho ...	2001	11311430
incidence of hantavirus infections in belgium.	over the last two decades, and from the moment that serological detection was possible, human hantavirus infections have been documented in most european countries. this paper summarises the available data on hantavirus cases in belgium. these data enable the demonstration of the existence of a 3-year epidemic cycle in belgium, which is apparently linked to rodent population dynamics.	2001	11451489
interaction between molecules of hantavirus nucleocapsid protein.	intermolecular interactions of tula hantavirus n (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. using chemical cross-linking and immunoblotting it was shown that the n protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of forming dimers, trimers and multimers, thus confirming the capacity of the protein molecules to interact wit ...	2001	11457990
mystery siliguri fever solved?		2001	11467154
[the worldwide challenges of "new" or reemerging communicable diseases at the dawn of the 21st century].	in the first part of this review, aids, prion diseases, hantavirus and arbovirus infections, ebola hemorrhagic fever, legionellosis, hepatitis c, enterotoxigenic escherichia coli infections, lyme disease, tuberculosis have provided alarming examples of emerging or reemerging infectious diseases. in this second part, the stress is placed on the reemergence of diphtheria and of serious streptococcal infections, on bartonelloses, chlamydia infections, fungal infections, while malaria and cholera ar ...	2001	11468579
do unusual site-specific population dynamics of rodent reservoirs provide clues to the natural history of hantaviruses?	between january 1995 and november 1997, longitudinal mark-recapture studies of rodent hosts of hantaviruses in a disturbed microhabitat within a shortgrass prairie ecosystem in southeastern colorado (usa) were conducted. the site was distinguished by edaphic and floristic characteristics unique to this area and associated with historical land use patterns, as well as the year-around availability of water from a functioning windmill. populations of two common rodent species that are hosts for han ...	2001	11310878
hantavirus infections: clinical presentation in the emergency room.	we present a retrospective review of hantavirus infection in the emergency department. thirteen cases of hantavirus infections with renal syndrome from july 1989 to august 1999 were analysed. the diagnosis was confirmed by detection of hantavirus antibodies in all cases. fever, chills and headaches were universally present. intense back pain was associated in 77% of the patients. thrombocytopenia, abnormal urinalysis, hypertransaminasaemia, increased lactate dehydrogenase were the principal biol ...	2001	11314815
[hantavirus pulmonary and cardiovascular syndrome: epidemiology, clinical presentation, laboratory diagnosis and treatment].	the hantavirus pulmonary and cardiovascular syndrome (hpcvs) is an emerging disease in brazil. in this study, eight confirmed cases of hpcvs were studied. all the patients presented fever and dyspnea as well as thrombocytopenia and hypoxemia. tachycardia, malaise, hypotension and lung rales occurred in 75 to 87.5% of the cases. hemoconcentration, blood cell count increased and immature neutrophils, and high levels of creatinine were observed in 75 to 87.5%. intravenous liquid infusion, the use o ...	2001	11340492
[respiratory manifestations of hemorrhagic fever with renal syndrome. retrospective study of 129 cases in champagne-ardenne and lorraine].	the contradiction between airborne transmission of puumala virus and the low rate of airway manifestations reported in hemorrhagic fever with renal syndrome (hfrs) caused by this virus led us to conduct this study to check whether the incidence of respiratory manifestations may have been underestimated.	2001	11244810
evaluation of a pan-reactive hantavirus enzyme immunoassay and of a hantavirus immunoblot for the diagnosis of nephropathia epidemica.	nephropathia epidemica (ne) caused by the hantavirus serotype puumala (puuv) is endemic in large parts of europe. the prognosis of this disease is usually good. however, a rapid serological diagnosis is important to differentiate ne from potentially more severe renal conditions.	2001	11255099
a serological survey of rural dogs and cats on the southwestern canadian prairie for zoonotic pathogens.	a survey for antibodies against agents of plague, tularemia, and rocky mountain spotted fever (rmsf), and against sin nombre hantavirus (snv), bartonella henselae and b. clarridgeiae was conducted in the summer of 1995 using serum from rural dogs and cats living in the vicinity of four public parks in southeastern alberta and southwestern saskatchewan. antibodies to all pathogens were detected in all survey areas. overall prevalence rates were 0.075 for yersinia pestis, 0.089 for francisella tul ...	2001	11257996
truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections.	truncated recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), seoul virus (seov), and dobrava virus (dobv) were expressed by a baculovirus system. the truncated rnps, which lacked 49 (rnp50) or 154 (rnp155) n-terminal amino acids of the nps of htnv, seov, and dobv, were able to differentiate htnv-, seov-, and dobv-specific immune sera. recombinant np50s retained higher reactivities than rnp155s and were proven useful for enzyme-linked immunosorbent assay (elisa). the elisas based o ...	2001	11427545
the english 'sweate' (sudor anglicus) and hantavirus pulmonary syndrome.	a rapidly fatal viral infectious disease appeared in england in 1485, persisted for the summer months and disappeared as winter approached. this pattern of infection re-appeared in 1508, 1517, 1528, and finally 1551. the epidemic never returned. it had no respect for wealth or rank, and predominantly attacked males between the ages of 15 and 45 years. the incubation period was frighteningly short and the outcome normally fatal. the symptoms of acute respiratory disease and copious sweating were ...	2001	11284216
serologic evidence of hantavirus infection in sigmodontine rodents in mexico.	antibodies to hantaviruses in two species of sigmodontine rodents (peromyscus maniculatus and reithrodontomys sumichrasti) collected in central mexico are reported. peromyscus maniculatus, a common species throughout much of mexico, is the reservoir of sin nombre virus (snv), the etiologic agent of the great majority of cases of hantavirus pulmonary syndrome (hps) in north america. although the identity of the virus detected in p. maniculatus in mexico could not be determined by these serologic ...	2001	11310896
prevalence of antibodies to sin nombre virus in humans living in rural areas of southern new mexico and western texas.		2001	11226584
modifying the cellular transport of dna-based vaccines alters the immune response to hantavirus nucleocapsid protein.	puumala virus is a member of the hantavirus genus (family bunyaviridae) and is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) in europe. a genetic vaccination approach was conducted to investigate if the immune response could be modulated using different cellular secretion and/or localisation signals, and the immune responses were analysed in balb/c mice and in a bank vole infectious model. rodents vaccinated with dna constructs encoding the antigen fused to an amino ...	2001	11427253
[the worldwide challenges of "new" or reemerging communicable diseases at the dawn of the 21st century].	in spite of the very significant advances made during the 20 th century in the prevention and the treatment of communicable diseases, infections are still today, even in developed countries, a major cause of morbidity and mortality. new infectious diseases have emerged (aids, legionellosis, exterotoxigenic e. coli, ebola fever), others have significantly reemerged (tuberculosis, diphtheria, bartonella infections) or have seen their geographic distribution widen considerably (dengue, hantavirus, ...	2001	11427818
characterization of in vitro and in vivo antiviral activity of lactoferrin and ribavirin upon hantavirus.	mechanisms of anti-hantaviral activities of bovine lactoferrin (lf) and ribavirin (rbv) were investigated. hantavirus focus formation at 48 hr was 15% of the control in cells treated with 400 microg/ml lf for 1 hr at 37 degrees c prior to viral infection. post infection treatment with 100 microg/ml rbv also inhibited the focus formation to 2.5% of the control. combined lf pre- and rbv post-infection treatment completely inhibited focus formation. viral glycoprotein (g2) and nucleocapsid protein ...	2001	11459009
health assessment of u.s. military personnel deployed to bosnia-herzegovina for operation joint endeavor.	in anticipation of u.s. forces deploying to bosnia-herzegovina, plans were established to conduct medical surveillance of all military service members. this surveillance would provide the department of defense with an overview of the hospitalization and outpatient morbidity experience of u.s. forces. standardized collection of medical data from all u.s. camps using 14 diagnostic categories based on international classification of diseases, 9th revision, codes began in march 1996. special assessm ...	2001	11413721
[epidemiology of hantaviruses in baden-wurttemberg].	hantavirus, originally named after the hantaan river in korea, is the aetiologic agent for the hemorrhagic fever with renal syndrome (hfrs) in the asian region, in the americas for the hantavirus pulmonary syndrome (hps). in middle europe hantaviruses are responsible for the "nephropathia epidemica" (ne), a mild form of hfrs. hantaviruses belong to the family of bunyaviridae. like other members of this family their genome consists of three segments of single stranded rna (ss-rna) leading to var ...	2001	11285748
geographical distribution of rodent-associated hantaviruses in texas.	the purpose of this study was to increase our knowledge of the geographic distribution and natural host range of hantaviruses in texas, southeastern new mexico, and mexico. blood samples from 3,225 wild rodents, representing 34 species, were tested for hantavirus antibody (igg), using an enzyme-linked immunosorbent assay. hantavirus antibody was found in one or more rodents from each of 13 counties in texas, otero county in southeastern new mexico, and mexico state (central mexico). the 133 anti ...	2001	11469186
hantavirus maturation.		2001	11217405
virus evolution and genetic diversity of hantaviruses and their rodent hosts.		2001	11217406
[hantavirus pulmonary syndrome in buenos aires province].	in argentina the first hantavirus pulmonary syndrome (hps) cases were characterized in 1995. since then, argentina is the country with the highest number of notified cases in south america. the disease is distributed in northern, southern and central regions of the country, being buenos aires the most affected province from the central region. in this study, we present seasonal and geographical distribution of hps cases in buenos aires province, the association with diverse viral lineages and th ...	2001	11374136
modeling hantavirus maintenance and transmission in rodent communities.		2001	11217407