| setting a trap for hantavirus. | | 2000 | 11022538 |
| [serological diagnosis and nasopharyngeal washings in pediatric infections]. | in the course of respiratory infections, the efficacy of microbiologic diagnosis has increased years after years, in term of specificity, sensitivity and rapidity. new pathogenic agents have been described such as: legionella pneumophila, chlamydia pneumoniae, hantavirus. some viruses have been well characterized as responsible for seasonal outbreaks using rapid tools for identification. needs for efficient diagnostic tools became more obvious when specific antiviral drugs appeared on the market ... | 2000 | 11028183 |
| [hantavirus: recent data and review of the literature]. | hantavirus (bunyaviridae family) are present worldwide and are the causative agent of haemorrhagic fever with renal or pulmonary syndrome. these viruses are transmitted by rodents with asymptomatic infection which thus act as an excellent reservoir. molecular epidemiology indicates that hantavirus may have evolved in three ways: mutations within the genome, reassortment of the segmented genome between two closely related hantavirus, and genomic recombination, a relatively rare phenomenon among n ... | 2000 | 11030053 |
| dna vaccination of mice with a plasmid encoding puumala hantavirus nucleocapsid protein mimics the b-cell response induced by virus infection. | inoculation of naked dna has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. to study the humoral immune response induced by dna vaccination we cloned the entire nucleocapsid protein-encoding sequence of the puumala hantavirus strain vranica/hällnäs into the cmv promoter-driven expression unit of the plasmid pcdna3, generating pcdna3-vr1. a single dose injection of 50 microg of plasmid dna into each m. tibialis anterior of balb/c mice ... | 2001 | 11035190 |
| hantavirus pulmonary syndrome is distinguishable from acute interstitial pneumonia. | hantavirus pulmonary syndrome (hps) and acute interstitial pneumonia (aip) can share similar clinical presentations. aip is an acute, diffuse lung disease that has some clinical features suggesting a viral infection, although causative agent(s) have not been identified. | 2000 | 11035576 |
| emerging viral diseases. | | 2000 | 11035785 |
| puumala (puu) hantavirus strain differences and insertion positions in the hepatitis b virus core antigen influence b-cell immunogenicity and protective potential of core-derived particles. | hepatitis b virus (hbv) core-derived chimeric particles carrying a puumala (puu) hantavirus (strain vranica/hällnäs) nucleocapsid (n) protein sequence (aa 1-45), alternatively inserted at three distinct positions (n-, c-terminus, or the internal region), and mosaic particles consisting of hbv core as well as core/puu (vranica/hällnäs) n (aa 1-45) readthrough protein were generated. chimeric particles carrying the insert at the n-terminus or the internal region of core induced some protective imm ... | 2000 | 11040127 |
| [hantavirus disease during pregnancy]. | hantavirus infection is known to occur in mice and rats without symptoms. in contrast, an infection with hantavirus in humans gives influenzalike symptoms and acute renal failure. the treatment is symptomatic, but treatment with dialysis can be necessary for a while. a case of hantavirus infection in denmark in a 30 week pregnant woman is reported. | 2000 | 11043050 |
| hantavirus pulmonary syndrome in northern alberta, canada: clinical and laboratory findings for 19 cases. | we reviewed the clinical and laboratory findings for 19 cases of hantavirus pulmonary syndrome (hps) identified either serologically or by immunohistochemical testing of archival tissue at our tertiary care center. fever (95%), cough (89%), and dyspnea (89%) were the most common presenting symptoms. the most prevalent presenting signs were respiratory abnormalities (95%) and tachycardia (84%). common laboratory findings included thrombocytopenia (95%) and leukocytosis (79%). elevated aspartate a ... | 2000 | 11049774 |
| [hantavirus pulmonary syndrome in southern argentina]. | andes virus was identified in 1995 as the etiologic agent of hantavirus pulmonary syndrome (hps) in southern argentina. we describe herein the main clinical characteristics of 25 hps confirmed cases acquired in this area between 1993 and september 1999. the mean age was 34 years (range 11-70), with 72% males. clinical characteristics were similar to those previously reported for sin nombre virus (snv) cases. however, in this group of patients we also observed conjuntival injection in 10/25 (42%) ... | 2000 | 11050803 |
| [prevalence of anti-hantavirus antibodies in health care personnel in direct contact with patients with hantavirus pulmonary syndrome in temuco, chile 1997 to 1999]. | an outbreak of hantavirus cardiopulmonary syndrome (hvcs) caused by the andes virus, affected chile since 1995. antibodies to hantavirus in health care workers who had cared patients with hvcs in coybaique, argentinean reports and familial clustering of bantaviral illness, raised the possibility of person to person transmission. familial clustering could occur secondary to a similar exposure to a common infected environment of more than one member of the family. moreover, the prevalence of antib ... | 2000 | 11050834 |
| hantavirus pulmonary syndrome in panama: identification of novel hantaviruses and their likely reservoirs. | hantavirus pulmonary syndrome (hps), a severe respiratory disease with high mortality caused by rodent-borne hantaviruses, has previously been identified in the united states and canada as well as central and southern south america. in late 1999 and early 2000, an outbreak of acute illness compatible with hps was reported in los santos, panama, with the death of 3 of the 12 (25%) suspected cases. hantavirus-specific antibodies were detected in patient sera, and virus rna was detected by reverse ... | 2000 | 11062031 |
| first case of puumala virus infection in greece. | | 2000 | 11073146 |
| genetic diversity of hantaviruses associated with hemorrhagic fever with renal syndrome in the far east of russia. | to identify the hantaviruses causing hemorrhagic fever with renal syndrome (hfrs) in the far east of russia, blood samples collected from hfrs patients in 1994-1998, were examined by reverse transcription-polymerase chain reaction. in addition, 36 sera were tested by an immunofluorescence assay for antibodies against hantaan, seoul, puumala, and khabarovsk viruses, and 54 samples were tested by plaque reduction neutralization test. with both serological assays, the highest antibody titers were t ... | 2000 | 11074123 |
| hantavirus pulmonary syndrome associated with monongahela virus, pennsylvania. | the first two recognized cases of rapidly fatal hantavirus pulmonary syndrome in pennsylvania occurred within an 8-month period in 1997. illness in the two patients was confirmed by immunohistochemical techniques on autopsy material. reverse transcription-polymerase chain reaction analysis of tissue from one patient and environmentally associated peromyscus leucopus (white-footed mouse) identified the monongahela virus variant. physicians should be vigilant for such monongahela virus-associated ... | 2000 | 11076720 |
| preliminary characterization and natural history of hantaviruses in rodents in northern greece. | | 2000 | 11076727 |
| evidence of puumala hantavirus infection in red foxes (vulpes vulpes) in belgium. | | 2000 | 11083049 |
| hantavirus pulmonary syndrome: an alert to latin american countries. | approximately 400 cases of hantavirus pulmonary syndrome (hps) have been reported on the north american continent since the first epidemic in 1993, in new mexico, usa. in brazil, 12 cases were confirmed before 1998. infection results from inhalation of rodent blood, saliva or waste. clinical findings of the disease are characterized by a nonspecific febrile illness, followed by rapid progression to pulmonary edema which may lead to a shock-like state or renal failure. mortality is high and emerg ... | 1999 | 11084670 |
| molecular evolution of puumala hantavirus in fennoscandia: phylogenetic analysis of strains from two recolonization routes, karelia and denmark. | like other members of the genus hantavirus: in the family bunyaviridae:, puumala virus (puuv) is thought to be co-evolving with its natural host, the bank vole clethrionomys glareolus. to gain insight into the evolutionary history of puuv in northern europe during the last post-glacial period, we have studied wild-type puuv strains originating from areas along two postulated immigration routes of bank voles to fennoscandia. full-length sequences of the s rna segment and partial sequences (nt 216 ... | 2000 | 11086113 |
| effects of tumor necrosis factor alpha on sin nombre virus infection in vitro. | previous data indicate that immune mechanisms may be involved in developing capillary leakage during sin nombre virus (snv) infection. therefore, we investigated production of tumor necrosis factor alpha (tnf-alpha) by human alveolar macrophages and human umbilical vein endothelial cells (huvec) after infection with snv. in addition, we examined the effect of tnf-alpha on huvec monolayer leakage. our results reveal that although tnf-alpha decreases accumulation of viral nucleoproteins, tnf-alpha ... | 2000 | 11090198 |
| [use of kinetic hemagglutination inhibition test in study of antigenic relationships of hantavirus strains circulating in foci of hemorrhagic fever with renal syndrome in southern far east of russia]. | the kinetics of reaction between hemagglutinating antigens and immune sera to 10 hantavirus strains (7 isolated in the primorye territory and 3 in other regions) was studied in the cross kinetic hemagglutination inhibition (khai) test. an a parameter is suggested for evaluation of the similarity and differences between the studied strains. this parameter helped detect antigenic differences between hantaan and seoul viruses and the inter-type differences between hantaan-like strains. khai is more ... | 2000 | 11107654 |
| [zoonoses among immigrants]. | during the last ten years denmark has received an increasing number of immigrants, especially from the balkans, the middle east, and somalia. some of these may suffer from the zoonoses occurring in their country of origin. but apart from echinococcosis, zoonoses in these immigrants do not seem to pose a quantitatively greater problem than in danes who have visited these areas. however it is important to have a knowledge of the symptoms and mode of transmission of zoonoses occurring in areas wher ... | 2000 | 11107966 |
| hantavirus infections among mammalogists studied by focus reduction neutralisation test. | | 2000 | 11117649 |
| genetic diversity of hantaviruses isolated in china and characterization of novel hantaviruses isolated from niviventer confucianus and rattus rattus. | the antigenic and genetic properties of 46 hantaviruses from china, 13 from patients, 23 from rodents, and 10 from unknown hosts, were compared with those of other hantaviruses. the viruses were classified as either hantaan (htn) or seoul (seo) viruses. a phylogenetic analysis of the partial m (300 bp) and s (around 485 bp) genomes of htn viruses identified nine distinct genetic subtypes, one consisting of isolates from korea. the seo viruses were divided into five genetic subtypes, although the ... | 2000 | 11118357 |
| expression of a human, neutralizing monoclonal antibody specific to puumala virus g2-protein in stably-transformed insect cells. | we cloned the heavy- and light-chain antibody genes of a human x (humanxmouse) trioma secreting a neutralizing, igg monoclonal antibody to the g2-protein of puumala virus. the antibody genes were inserted separately into plasmid transfer vector piei-4 such that the genes were under control of the baculovirus immediate early gene promoter, iei. trichoplusia ni (tn) cells were co-transfected with these constructs and a selection plasmid containing a neomycin-resistance gene. cloned transformants e ... | 2000 | 11121551 |
| t-helper and humoral responses to puumala hantavirus nucleocapsid protein: identification of t-helper epitopes in a mouse model. | puumala hantavirus (puuv) is a rodent-borne agent causing nephropathia epidemica in humans, a milder form of haemorrhagic fever with renal syndrome occurring in fennoscandia, central europe and western russia. in this study we characterized the immunogenicity of an e. coli-expressed nucleocapsid (n) protein of puuv (strain kazan-e6) in inbred mice (balb/c, cba and c57/bl6). the recombinant n (rn) protein raised puuv-specific antibodies in all three tested murine haplotypes, and all igg subclasse ... | 2001 | 11125166 |
| serological study of hantavirus in the rodent population of nakhon pathom and nakhon ratchasima provinces thailand. | a serological survey has been carried out to detect evidence of hantavirus infection in rodents from two provinces of thailand. this study aimed to examine virus antibody in 354 rodents trapped among 6 different villages of nakhon pathom province (february-march, 1998) and in 326 rodents trapped among 14 villages of nakhon ratchasima province (august-october, 1998). seroprevalence among rodents from nakhon pathom province (2.3%), was mostly find in rattus norvegicus (3.8%) and bandicota indica ( ... | 2000 | 11127326 |
| genetic diversity of apodemus agrarius-borne hantaan virus in korea. | hantaan (htn) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (hfrs), was first isolated in 1976 from lung tissues of striped field mice (apodemus agrarius) captured in songnae-ri, kyungki-do, korea. to clarify the genetic diversity and phylogenetic relationship among korean strains of htn virus, viral sequences of the partial s and m segments were amplified from lung tissues of 24 seropositive striped field mice captured between 1989 and 1998 at 11 sites in ... | 2000 | 11129640 |
| lack of evidence for an association between hantavirus infections and wegener's granulomatosis, microscopic polyangiitis, churg-strauss syndrome and giant cell arteritis. | | 2000 | 11136889 |
| hantavirus nucleocapsid protein is expressed as a membrane-associated protein in the perinuclear region. | black creek canal virus (bccv) is a new world hantavirus which is associated with hantavirus pulmonary syndrome. we have examined the site of expression of the bccv nucleocapsid protein (nbccv) in the absence of bccv glycoproteins and found that the majority of the protein is localized to the golgi region. immunofluorescence analysis of bhk21 cells expressing the nbccv and la crosse virus nucleocapsid protein (nlacv) showed different intracellular localization patterns of these proteins within t ... | 2001 | 11160679 |
| hantavirus nucleocapsid protein oligomerization. | hantaviruses are enveloped, negative-strand rna viruses which can be lethal to humans, causing either a hemorrhagic fever with renal syndrome or a hantaviral pulmonary syndrome. the viral genomes consist of three rna segments: the l segment encodes the viral polymerase, the m segment encodes the viral surface glycoproteins g1 and g2, and the s segment encodes the nucleocapsid (n) protein. the n protein is a 420- to 430-residue, 50-kda protein which appears to direct hantavirus assembly, although ... | 2001 | 11160704 |
| dobrava hantavirus causes hemorrhagic fever with renal syndrome in central europe and is carried by two different apodemus mice species. | in central europe, hemorrhagic fevers with renal syndrome (hfrs) in humans are caused by the hantavirus species puumala (transmitted by voles) and a second, hantaan-related species (transmitted by mice). the second virus could be identified as dobrava virus. to date, 19 clinical cases of dobrava infection have been found in germany and slovakia. all patients exhibited a mild/moderate clinical course and no case fatality occurred. screening for infected rodents revealed that the striped field mou ... | 2001 | 11170053 |
| risks and prevention of nosocomial transmission of rare zoonotic diseases. | americans are increasingly exposed to exotic zoonotic diseases through travel, contact with exotic pets, occupational exposure, and leisure pursuits. appropriate isolation precautions are required to prevent nosocomial transmission of rare zoonotic diseases for which person-to-person transmission has been documented. this minireview provides guidelines for the isolation of patients and management of staff exposed to the following infectious diseases with documented person-to-person transmission: ... | 2001 | 11170953 |
| [febrile cytolysis disclosing hemorrhagic fever with renal syndrome]. | we report the case of a 40-year-old-man who developed febrile cytolysis as the presenting sign of hemorragic fever with renal syndrome (hfrs). on admission, he had fever (40 degrees c) and epigastric pain. the ast level was at 2n, the alt at 3n. there was a thrombocytopenia (61 000/mm(3)) without anemia or hyperleukocytosis. three days after admission, the platelet count decreased to 40 000/mm(3), serum urea and creatinine increased from normal rate to 10.8mmol/l, 204.0 micromol/l, respectively. ... | 2000 | 11173712 |
| hantavirus infections. | hantaviruses are the causative agents of the zoonotic diseases known as haemorrhagic fever with renal syndrome (hfrs) in europe and asia, and hantavirus pulmonary syndrome (hps) in the americas. these pathogens are maintained in the wild by rodent reservoirs and are mainly transmitted via the aerosol route. the infection is chronic and apparently asymptomatic in host animals. whilst hfrs is caused by hantaan, seoul, dobrava and puumala hantaviruses, hps is associated with sin nombre-like viruses ... | 2000 | 11189727 |
| puumala and dobrava hantaviruses causing hemorrhagic fever with renal syndrome in estonia. | | 2000 | 11205639 |
| [adaptive property of hantaviruses during their isolation from cultured vero-e6 cells in relation to the type of the ecological host]. | as a result of virological studies, 185 lung tissue specimens from 4 rodent species caught near khabarovsk were isolated and fixed in the passages of cultured vero-6 cells of 68 hantavirus strains. the capacity of the strains to adapt to the cells was assessed by using the adaptive index involving the mean rates of successful isolation, its duration, hantavirus antigen titer in the material used for infection. the strains of hantavirus serotypes were noted for the highest adaptive properties, wh ... | 2000 | 11210409 |
| expression of immunogenic puumala virus nucleocapsid protein in transgenic tobacco and potato plants. | transgenic plants, expressing recombinant proteins, are suitable alternatives for the production of relevant immunogens. in the present study, the expression of puumala virus nucleocapsid protein in tobacco and potato plants (nicotiana tabacum and solanum tuberosum) and its immunogenicity was investigated. after infection of leaf discs of sr1 tobacco and tuber discs of potato cv. "desiree" with the agrobacterium strain lba4404 (pal4404, pbinar-puu-s) containing the 1302 bp cdna sequence of s-rna ... | 2001 | 11210942 |
| hantaviruses: history and overview. | | 2001 | 11217399 |
| clinical manifestations of new world hantaviruses. | | 2001 | 11217400 |
| clinical characteristics of hantavirus infections on the eurasian continent. | | 2001 | 11217401 |
| replication of hantaviruses. | | 2001 | 11217402 |
| human immune response, host genetics, and severity of disease. | | 2001 | 11217403 |
| vaccines against hantaviruses. | | 2001 | 11217404 |
| hantavirus maturation. | | 2001 | 11217405 |
| virus evolution and genetic diversity of hantaviruses and their rodent hosts. | | 2001 | 11217406 |
| modeling hantavirus maintenance and transmission in rodent communities. | | 2001 | 11217407 |
| cellular receptors and hantavirus pathogenesis. | hantaviruses cause two potentially lethal diseases, hps and hfrs, and both diseases result in defects in vascular permeability and platelet function. human beta 3 integrins confer cellular susceptibility to hps- and hfrs-causing hantaviruses, a fact directly linking platelets, endothelial cells, and hantavirus diseases to the use of [figure: see text] cellular receptors that maintain capillary integrity and regulate platelet function. the role of vitronectin, pai-1, upar, and complement cascades ... | 2001 | 11217408 |
| new and emerging pathogens. | | 2000 | 11219303 |
| genetic characterization of puumala hantavirus strains from belgium: evidence for a distinct phylogenetic lineage. | puumala hantavirus (puuv) sequences were recovered from red bank voles (clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern belgium: thuin, montbliart, momignies and couvin. in addition, three puuv isolates originating from bank voles trapped in the 1980s in southern (montbliart) and northern (turnhout) belgium were genetically characterized. analysis of the complete s and partial m segment sequences showed that the belgian puuv strains constitute a genetic line ... | 2001 | 11226569 |
| prevalence of antibodies to sin nombre virus in humans living in rural areas of southern new mexico and western texas. | | 2001 | 11226584 |
| [analysis of the primary structure of hantavirus rna, detected in organs of patients that died from hfks in the republic of bashkortostan]. | fragments of the minor s and middle m segments of hantavirus genome rna were isolated by reverse transcription-polymerase chain reaction (rt-pcr) from the organs of patients who died from hemorrhagic fever with the renal syndrome (hfrs) during an epidemic outbreak of this infection in the bashkortostan republic in 1997-1998. the sizes of the resultant pcr fragments were 317 p. n. for s segment and 416 p. n. for m segment. their primary structures (genbank registration numbers aj133581 and aj1335 ... | 2001 | 11236741 |
| exotic diseases. are they spreading to a town near you? | | 2001 | 11242844 |
| [respiratory manifestations of hemorrhagic fever with renal syndrome. retrospective study of 129 cases in champagne-ardenne and lorraine]. | the contradiction between airborne transmission of puumala virus and the low rate of airway manifestations reported in hemorrhagic fever with renal syndrome (hfrs) caused by this virus led us to conduct this study to check whether the incidence of respiratory manifestations may have been underestimated. | 2001 | 11244810 |
| hantavirus. | although hantaviruses have been known to exist for over 40 years, they were thought only to be the cause of hemorrhagic fever and renal syndrome (hfrs) in europe and asia. rodents are the primary hosts of hantaviruses, and humans become infected mainly by inhalation of viral particles in the rodent excreta. the united states was thought to be spared from pathologic hantaviruses until the summer of 1993, when an outbreak of acute respiratory distress syndrome occurred in the four corners region o ... | 2001 | 11246028 |
| evaluation of a pan-reactive hantavirus enzyme immunoassay and of a hantavirus immunoblot for the diagnosis of nephropathia epidemica. | nephropathia epidemica (ne) caused by the hantavirus serotype puumala (puuv) is endemic in large parts of europe. the prognosis of this disease is usually good. however, a rapid serological diagnosis is important to differentiate ne from potentially more severe renal conditions. | 2001 | 11255099 |
| a serological survey of rural dogs and cats on the southwestern canadian prairie for zoonotic pathogens. | a survey for antibodies against agents of plague, tularemia, and rocky mountain spotted fever (rmsf), and against sin nombre hantavirus (snv), bartonella henselae and b. clarridgeiae was conducted in the summer of 1995 using serum from rural dogs and cats living in the vicinity of four public parks in southeastern alberta and southwestern saskatchewan. antibodies to all pathogens were detected in all survey areas. overall prevalence rates were 0.075 for yersinia pestis, 0.089 for francisella tul ... | 2001 | 11257996 |
| mouse mx2 protein inhibits hantavirus but not influenza virus replication. | the antiviral potential of mx2 protein remains unknown, because the mx2 gene in commonly used strains of laboratory mice is nonfunctional. our previous study showed that functional mx2 protein in some feral-origin strains was induced upon interferon treatment, was localized in the cytoplasm, and inhibited vesicular stomatitis virus replication. in the present study, we have demonstrated that the embryonic fibroblastic cells from a feral-origin strain (spr) expressed 74 kda mx2 protein, which pre ... | 2001 | 11266216 |
| the english 'sweate' (sudor anglicus) and hantavirus pulmonary syndrome. | a rapidly fatal viral infectious disease appeared in england in 1485, persisted for the summer months and disappeared as winter approached. this pattern of infection re-appeared in 1508, 1517, 1528, and finally 1551. the epidemic never returned. it had no respect for wealth or rank, and predominantly attacked males between the ages of 15 and 45 years. the incubation period was frighteningly short and the outcome normally fatal. the symptoms of acute respiratory disease and copious sweating were ... | 2001 | 11284216 |
| [epidemiology of hantaviruses in baden-wurttemberg]. | hantavirus, originally named after the hantaan river in korea, is the aetiologic agent for the hemorrhagic fever with renal syndrome (hfrs) in the asian region, in the americas for the hantavirus pulmonary syndrome (hps). in middle europe hantaviruses are responsible for the "nephropathia epidemica" (ne), a mild form of hfrs. hantaviruses belong to the family of bunyaviridae. like other members of this family their genome consists of three segments of single stranded rna (ss-rna) leading to var ... | 2001 | 11285748 |
| the incubation period of hantavirus pulmonary syndrome. | in 1993 sin nombre virus was recognized as the cause of hantavirus pulmonary syndrome (hps) and the deer mouse (peromyscus maniculatus) was identified as the reservoir host. surveillance by the centers for disease control and prevention and state health departments includes investigation to determine the likely site(s) and activities that led to infection, an environmental assessment of the home and workplace, and possibly rodent trappings at these sites. as of december 31, 1998, there were 200 ... | 2000 | 11304061 |
| do unusual site-specific population dynamics of rodent reservoirs provide clues to the natural history of hantaviruses? | between january 1995 and november 1997, longitudinal mark-recapture studies of rodent hosts of hantaviruses in a disturbed microhabitat within a shortgrass prairie ecosystem in southeastern colorado (usa) were conducted. the site was distinguished by edaphic and floristic characteristics unique to this area and associated with historical land use patterns, as well as the year-around availability of water from a functioning windmill. populations of two common rodent species that are hosts for han ... | 2001 | 11310878 |
| serologic evidence of hantavirus infection in sigmodontine rodents in mexico. | antibodies to hantaviruses in two species of sigmodontine rodents (peromyscus maniculatus and reithrodontomys sumichrasti) collected in central mexico are reported. peromyscus maniculatus, a common species throughout much of mexico, is the reservoir of sin nombre virus (snv), the etiologic agent of the great majority of cases of hantavirus pulmonary syndrome (hps) in north america. although the identity of the virus detected in p. maniculatus in mexico could not be determined by these serologic ... | 2001 | 11310896 |
| genetic and serotypic characterization of sin nombre-like viruses in canadian peromyscus maniculatus mice. | in canada, hantavirus infected deer mice (peromyscus maniculatus) have been collected from british columbia to newfoundland. partial sequencing of g1 and n protein encoding regions from canadian peromyscus maniculatus-borne hantaviruses demonstrated the existence of significant genotypic divergence among strains. phylogenetic analysis showed that sin nombre (sn)-like viruses from eastern and western canadian deer mice can be divided into at least two broad-based genogroups. sequencing of mitocho ... | 2001 | 11311430 |
| hantavirus infections: clinical presentation in the emergency room. | we present a retrospective review of hantavirus infection in the emergency department. thirteen cases of hantavirus infections with renal syndrome from july 1989 to august 1999 were analysed. the diagnosis was confirmed by detection of hantavirus antibodies in all cases. fever, chills and headaches were universally present. intense back pain was associated in 77% of the patients. thrombocytopenia, abnormal urinalysis, hypertransaminasaemia, increased lactate dehydrogenase were the principal biol ... | 2001 | 11314815 |
| molecular cloning and high-level expression of g2 protein of hantaan (htn) virus 76-118 strain in the yeast pichia pastoris km71. | hantaan viral g2 envelope gene, which is known to be one of major antigens and induce neutralizing antibodies, was cloned into expression vector phil-s1 which consists of aox1 promoter, pho1 signal sequence, his4 gene and other components. the recombined plasmid was transformed into methylotropic yeast, pichia pastoris of km71 and recombinant strains harboring multi-copy of g2 gene were selected. expression of the cloned g2 gene was confirmed with western blot analysis using anti-sera of guinea ... | 2001 | 11324753 |
| [hantavirus pulmonary and cardiovascular syndrome: epidemiology, clinical presentation, laboratory diagnosis and treatment]. | the hantavirus pulmonary and cardiovascular syndrome (hpcvs) is an emerging disease in brazil. in this study, eight confirmed cases of hpcvs were studied. all the patients presented fever and dyspnea as well as thrombocytopenia and hypoxemia. tachycardia, malaise, hypotension and lung rales occurred in 75 to 87.5% of the cases. hemoconcentration, blood cell count increased and immature neutrophils, and high levels of creatinine were observed in 75 to 87.5%. intravenous liquid infusion, the use o ... | 2001 | 11340492 |
| emerging infectious diseases: a cause for concern. | as the twenty-first century begins it becomes increasingly apparent that the twentieth century, which opened with the promise of the eradication of most infectious diseases, closed with the specter of the reemergence of many deadly infectious diseases that have a rapidly increasing incidence and geographic range. equally if not more alarming is the appearance of new infectious diseases that have become major sources of morbidity and mortality. among recent examples are hiv/aids, hantavirus pulmo ... | 2000 | 11344776 |
| climate variability and change in the united states: potential impacts on vector- and rodent-borne diseases. | diseases such as plague, typhus, malaria, yellow fever, and dengue fever, transmitted between humans by blood-feeding arthropods, were once common in the united states. many of these diseases are no longer present, mainly because of changes in land use, agricultural methods, residential patterns, human behavior, and vector control. however, diseases that may be transmitted to humans from wild birds or mammals (zoonoses) continue to circulate in nature in many parts of the country. most vector-bo ... | 2001 | 11359689 |
| [hantavirus pulmonary syndrome in buenos aires province]. | in argentina the first hantavirus pulmonary syndrome (hps) cases were characterized in 1995. since then, argentina is the country with the highest number of notified cases in south america. the disease is distributed in northern, southern and central regions of the country, being buenos aires the most affected province from the central region. in this study, we present seasonal and geographical distribution of hps cases in buenos aires province, the association with diverse viral lineages and th ... | 2001 | 11374136 |
| new immunochromatographic rapid test for diagnosis of acute puumala virus infection. | a new immunochromatographic rapid test, poc puumala (erilab ltd., kuopio, finland), for detection of acute-phase puumala virus (puuv) infection was developed based on a highly purified baculovirus-expressed puuv nucleocapsid protein antigen and lateral immunodiffusion techniques. after addition of sample (5 microl of serum, plasma, or fingertip blood) and buffer, puuv-specific immunoglobulin m (igm) antibodies, if present, together with the gold-conjugated anti-human igm, formed a specific color ... | 2001 | 11376049 |
| isolation of dobrava virus from apodemus flavicollis in greece. | dobrava virus (dobv) carried by apodemus flavicollis is the causative agent of severe hemorrhagic fever with renal syndrome (hfrs). dobv was isolated from an a. flavicollis mouse trapped in northeastern greece. this is the third dobv cell culture isolate in the world, clustering together with other greek dobv sequences from hfrs patients and rodents. | 2001 | 11376073 |
| physicians' database searches as a tool for early detection of epidemics. | we analyzed retrospectively the use of physician desk reference database searches to identify epidemics of tularemia, nephropathy, pogosta disease, and lyme disease and compared the searches with mandatory laboratory reports to the national infectious diseases register in finland during 1995. continuous recording of such searches may be a tool for early detection of epidemics. | 2001 | 11384534 |
| high prevalence of sin nombre virus in rodent populations, central utah: a consequence of human disturbance? | | 2001 | 11384538 |
| hantavirus seroconversion of wild-caught peromyscus during quarantine. | | 2001 | 11384539 |
| hantavirus pulmonary syndrome in the united states. | since the first outbreak of hantavirus pulmonary syndrome (hps) in 1993, understanding of the vast distribution and potential impact of hantaviruses has grown. at least 277 cases of hps have been documented in the united states. the full clinical spectrum has yet to be elucidated, and one outbreak suggested the possibility of person-to-person transmission. new research has identified the b-3 integrins as cellular receptors for hantaviruses and has determined the pivotal role of the immune system ... | 2001 | 11384556 |
| hantavirus infection induces the expression of rantes and ip-10 without causing increased permeability in human lung microvascular endothelial cells. | sin nombre virus (snv) and hantaan virus (htn) infect endothelial cells and are associated with different patterns of increased vascular permeability during human disease. it is thought that such patterns of increased vascular permeability are a consequence of endothelial activation and subsequent dysfunction mediated by differential immune responses to hantavirus infection. in this study, the ability of hantavirus to directly induce activation of human lung microvascular endothelial cells (hmve ... | 2001 | 11390609 |
| clinical characterization of dobrava hantavirus infections in germany. | there is increasing evidence that dobrava (dobv) but not hantaan (htnv) hantavirus is a hemorrhagic fever with renal syndrome (hfrs) causing agent in central europe. however, only single clinical cases of hfrs due to acute dobv infection have been described so far. we report on three male patients from a non-endemic hantavirus focus in northern germany who suffered from mild to moderate hfrs strongly resembling nephropathia epidemica. serotyping by detection of hantavirus species-specific neutra ... | 2001 | 11393382 |
| molecular epidemiology of viral pathogens and tracing of transmission routes: hepatitis-, calici- and hantaviruses. | background: the need to rapidly identify new therapeutic drugs and vaccines for clinically important viral infections has resulted in intensive study of the molecular properties of viruses. modern molecular techniques have provided tools for tracing infections and studying the evolution of viruses. objective study and design: two examples illustrating how modern molecular techniques can be used in clinical virology and molecular epidemiology (hepatitis and caliciviruses), and one example documen ... | 2001 | 11397654 |
| health assessment of u.s. military personnel deployed to bosnia-herzegovina for operation joint endeavor. | in anticipation of u.s. forces deploying to bosnia-herzegovina, plans were established to conduct medical surveillance of all military service members. this surveillance would provide the department of defense with an overview of the hospitalization and outpatient morbidity experience of u.s. forces. standardized collection of medical data from all u.s. camps using 14 diagnostic categories based on international classification of diseases, 9th revision, codes began in march 1996. special assessm ... | 2001 | 11413721 |
| predominant kidney involvement in a fatal case of hantavirus pulmonary syndrome caused by sin nombre virus. | a 27-year-old woman presented to a hospital with symptoms resembling pyelonephritis; respiratory distress did not develop until nearly a day after admission and she subsequently died. the unexplained deaths and critical illnesses project of the centers for disease control and prevention confirmed sin nombre virus infection by the results of serological testing and sequencing of the viral genome; staining of sin nombre virus antigen in the pulmonary capillaries was relatively weak. | 2001 | 11418889 |
| [regional differences and hantavirus pulmonary syndrome]. | factors related to the characteristics of hantavirus pulmonary syndrome in argentina are described. factors from different scientific fields converge to form the syndrome's analytical framework. some of these factors are the history of spatial occupation, work and production structures, human migration patterns, ethnic composition, reservoir dynamics and its relationship to the different circulating viruses, and human behavior. furthermore, the multiple factors are expressed in three ecological ... | 2001 | 11426265 |
| modifying the cellular transport of dna-based vaccines alters the immune response to hantavirus nucleocapsid protein. | puumala virus is a member of the hantavirus genus (family bunyaviridae) and is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) in europe. a genetic vaccination approach was conducted to investigate if the immune response could be modulated using different cellular secretion and/or localisation signals, and the immune responses were analysed in balb/c mice and in a bank vole infectious model. rodents vaccinated with dna constructs encoding the antigen fused to an amino ... | 2001 | 11427253 |
| truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections. | truncated recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), seoul virus (seov), and dobrava virus (dobv) were expressed by a baculovirus system. the truncated rnps, which lacked 49 (rnp50) or 154 (rnp155) n-terminal amino acids of the nps of htnv, seov, and dobv, were able to differentiate htnv-, seov-, and dobv-specific immune sera. recombinant np50s retained higher reactivities than rnp155s and were proven useful for enzyme-linked immunosorbent assay (elisa). the elisas based o ... | 2001 | 11427545 |
| polymorphism of the cytokine genes in hospitalized patients with puumala hantavirus infection. | nephropathia epidemica (ne) is a mild type of haemorrhagic fever with renal syndrome caused by puumala (puu) hantavirus. the clinical course of ne varies from asymptomatic to fatal. the aim of this study was to establish whether polymorphisms in the cytokine genes are associated with susceptibility to and outcome of ne. | 2001 | 11427627 |
| [the worldwide challenges of "new" or reemerging communicable diseases at the dawn of the 21st century]. | in spite of the very significant advances made during the 20 th century in the prevention and the treatment of communicable diseases, infections are still today, even in developed countries, a major cause of morbidity and mortality. new infectious diseases have emerged (aids, legionellosis, exterotoxigenic e. coli, ebola fever), others have significantly reemerged (tuberculosis, diphtheria, bartonella infections) or have seen their geographic distribution widen considerably (dengue, hantavirus, ... | 2001 | 11427818 |
| [complete genome sequence analysis of the hantavirus z10 strain]. | study on the complete genome sequence of hantavirus z10 strain which has been applied for inactivated vaccine production in china, to assess its molecular characteristics and the diversity with other hantaviruses. | 2001 | 11436636 |
| antibody to sin nombre virus in rodents associated with peridomestic habitats in west central montana. | most human cases of hantavirus pulmonary syndrome are acquired in the peridomestic environment, yet studies of the ecology and infection dynamics in the reservoir host, the deer mouse (peromyscus maniculatus), have focused on sylvan populations. we describe a 2.5-year study of hantavirus infection in rodents associated with peridomestic habitats in west central montana. antibodies reactive with sin nombre virus (snv) were found in five species. overall snv antibody prevalence was highest among d ... | 2001 | 11442208 |
| incidence of hantavirus infections in belgium. | over the last two decades, and from the moment that serological detection was possible, human hantavirus infections have been documented in most european countries. this paper summarises the available data on hantavirus cases in belgium. these data enable the demonstration of the existence of a 3-year epidemic cycle in belgium, which is apparently linked to rodent population dynamics. | 2001 | 11451489 |
| interaction between molecules of hantavirus nucleocapsid protein. | intermolecular interactions of tula hantavirus n (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. using chemical cross-linking and immunoblotting it was shown that the n protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of forming dimers, trimers and multimers, thus confirming the capacity of the protein molecules to interact wit ... | 2001 | 11457990 |
| characterization of in vitro and in vivo antiviral activity of lactoferrin and ribavirin upon hantavirus. | mechanisms of anti-hantaviral activities of bovine lactoferrin (lf) and ribavirin (rbv) were investigated. hantavirus focus formation at 48 hr was 15% of the control in cells treated with 400 microg/ml lf for 1 hr at 37 degrees c prior to viral infection. post infection treatment with 100 microg/ml rbv also inhibited the focus formation to 2.5% of the control. combined lf pre- and rbv post-infection treatment completely inhibited focus formation. viral glycoprotein (g2) and nucleocapsid protein ... | 2001 | 11459009 |
| mystery siliguri fever solved? | | 2001 | 11467154 |
| [the worldwide challenges of "new" or reemerging communicable diseases at the dawn of the 21st century]. | in the first part of this review, aids, prion diseases, hantavirus and arbovirus infections, ebola hemorrhagic fever, legionellosis, hepatitis c, enterotoxigenic escherichia coli infections, lyme disease, tuberculosis have provided alarming examples of emerging or reemerging infectious diseases. in this second part, the stress is placed on the reemergence of diphtheria and of serious streptococcal infections, on bartonelloses, chlamydia infections, fungal infections, while malaria and cholera ar ... | 2001 | 11468579 |
| geographical distribution of rodent-associated hantaviruses in texas. | the purpose of this study was to increase our knowledge of the geographic distribution and natural host range of hantaviruses in texas, southeastern new mexico, and mexico. blood samples from 3,225 wild rodents, representing 34 species, were tested for hantavirus antibody (igg), using an enzyme-linked immunosorbent assay. hantavirus antibody was found in one or more rodents from each of 13 counties in texas, otero county in southeastern new mexico, and mexico state (central mexico). the 133 anti ... | 2001 | 11469186 |
| [discovery of a new subtype of seo virus]. | the m and s segments of cou3, a hantavirus strain isolated from rattus norvegieus were partially reverse transcripted, pcr amplified and sequenced with abi 373 dna sequencer to observe the differences from other seo type viruses. | 2000 | 11471015 |
| unexplained deaths and critical illnesses project (unex). | | 2001 | 11474630 |
| hantavirus pulmonary syndrome--vermont, 2000. | in 1993, an outbreak of an unexplained pulmonary illness occurred in the southwestern united states. this outbreak led to the first description of hantavirus pulmonary syndrome (hps), a rodentborne hantaviral infection. hantaviruses have been found in rodents in rural areas throughout the united states, but most infection has occurred in the southwest. this report describes the first hps case in vermont and underscores the importance of preventing exposure to peridomestic rodents and recognizing ... | 2001 | 11476530 |
| infection with sin nombre hantavirus: clinical presentation and outcome in children and adolescents. | sin nombre hantavirus (snv) is the leading causative agent of hantavirus cardiopulmonary syndrome (hcps) in the united states and canada. relatively few cases of hcps have involved children. this report describes the clinical characteristics of a series of pediatric cases of snv infection in the united states and canada from 1993 through march 2000. | 2001 | 11483837 |
| genetic identification and characterization of limestone canyon virus, a unique peromyscus-borne hantavirus. | hantaviruses, family bunyaviridae, are rodent-borne rna viruses that can cause hantavirus pulmonary syndrome (hps) in various regions of the americas. a coevolutionary relationship exists between hantaviruses and their specific rodent reservoir hosts; the phylogeny of the viruses generally matches that of the rodents. there are several peromyscus-borne hantaviruses, including sin nombre virus, the most common cause of hps in north america. this report describes the genetic detection and characte ... | 2001 | 11485402 |
| highly sensitive taqman pcr detection of puumala hantavirus. | an increasing number of clinical cases of hantavirus infections have been reported from various regions in asia, europe and north america. hantaviruses (family bunyaviridae, genus hantavirus) are enveloped and possess a single-stranded trisegmented rna genome of negative polarity. rodents or insectivores are natural hosts of hantaviruses and transmit the virus to humans chiefly by aerosolisation. these viruses are the causative agents of haemorrhagic fever with renal and pulmonary syndromes. in ... | 2001 | 11489422 |