| the first human isolate of puumala virus in scandinavia as cultured from phytohemagglutinin stimulated leucocytes. | a virus isolate was recovered from blood leucocytes of a patient with nephropathia epidemica (ne). leucocytes were isolated from edta-blood by dextran sedimentation and cultured on monolayers of vero e6 cells in the presence of phytohemagglutinin (pha) in roller tubes during the first 72 hours of incubation followed by rolling culture for three weeks in total. thereafter the first subculture was done in a plastic flask and afterward at at least 6 week intervals. antigen was first detected after ... | 1997 | 9334926 |
| puumala virus and two genetic variants of tula virus are present in austrian rodents. | puumala and tula viruses are hantaviruses found in europe and are associated with the rodents clethrionomys glareolus and microtus arvalis, respectively. puumala virus is associated with the human disease nephropathia epidemica. in austria, ten clinically diagnosed cases of nephropathia epidemica, presumably caused by puumala virus infection, have been reported but not virologically confirmed [leschinskaya et al., 1991; aberle et al., 1996]. to identify the hantaviruses that are present in austr ... | 1997 | 9334930 |
| effectiveness of recorded messages to communicate the risk of acquiring hantavirus pulmonary syndrome. | to determine the effectiveness of a recorded information line in communicating health risk during the emergence of a new disease, hantavirus pulmonary syndrome (hps), in the edmonton area and to study the accuracy of recall of information about hantavirus among the general public. | 1997 | 9336097 |
| hantavirus pulmonary syndrome -- chile, 1997. | hantavirus pulmonary syndrome (hps) was first recognized in chile in october 1995; through july 1997, nine cases had been identified in the country. however, during august 1-october 8, 1997, a total of 12 persons with hps, including two family clusters, were recognized. a collaborative investigation is under way to determine the magnitude of this outbreak, the associated rodent reservoir for hantaviruses, and the major risk factors for human infection. this report summarizes the preliminary resu ... | 1997 | 9338456 |
| hantavirus pulmonary syndrome in the americas. | | 1997 | 9341305 |
| glomerular basement membrane antibodies in hantavirus disease (hemorrhagic fever with renal syndrome). | the prevalence of antibodies against glomerular basement membrane (gbm) antigens in sera from 47 patients with serologically verified hantavirus (puumala serotype) infection were investigated. antibodies were measured by immunoassays using as antigen a crude human gbm preparation and the nc1 portion of type iv collagen (goodpasture antigen), respectively. seventy-seven percent of the patients had igm antibodies against the non-goodpasture glomerular basement membrane (non-gp gbm) in their acute ... | 1997 | 9342483 |
| isolation, genetic diversity, and geographic distribution of bayou virus (bunyaviridae: hantavirus). | bayou hantavirus, previously implicated in human hantavirus pulmonary syndrome in louisiana, was isolated from a rice rat (oryzomys palustris) captured in georgia. the presence of antibody among rice rats captured throughout the southeastern united states and the extent of diversity among the genetic variants of bayou viruses suggest that the rice rat is the most likely natural reservoir of the virus and that both virus and host have probably co-evolved for some years. | 1997 | 9347961 |
| nephropathia epidemica infection during first trimester of pregnancy--normal fetal outcome. | | 1997 | 9351417 |
| papillary necrosis: a late complication of nephropathia epidemica? | in the following we describe a case of nephropathia epidemica, which exhibited, a few years after acute infection, arterial hypertension and multiple papillary necrosis. papillary necrosis was diagnosed by i.v. pyelography and ct scan. a complete evaluation of the patient failed to show any other etiology for medullary necrosis. if arterial hypertension has already been reported as a complication of nephropathia epidemica, papillary necrosis is exceptional, but may be explained by the severe vas ... | 1997 | 9352163 |
| prevalence of serum antibodies to hantaviruses in northern sweden as measured by recombinant nucleocapsid proteins. | an enzyme-linked immunosorbent assay (elisa) based on recombinant nucleocapsid protein (rn delta) (aa 1-117) of hantaan, seoul, dobrava, sin nombre and puumala hantaviruses was used to determine the prevalence of antibodies among randomized and stratified individuals from northern sweden. in total, 137/1533 individuals (8.9%) had specific serum igg antibodies to puumala virus, the only hantavirus known to occur in the region. the prevalence of antibodies to puumala virus (8.9%) was determined to ... | 1997 | 9360248 |
| hantavirus infection in scid mice. | severe combined immunodeficiency (scid) mice were inoculated with hantaan virus strain 76-118 (htn) or seoul virus strain sr-11 (sr) of hantaviruses. susceptibility of scid mice was compared with those of immunocompetent adult mice, newborn mice and nude mice. scid mice inoculated with htn or sr died 32 to 35 days after infection. unlike newborn mice which also died of hantavirus infection, scid mice survived longer than newborn mice and showed typical wasting symptoms rather than nervous sympto ... | 1997 | 9362032 |
| diseases of the pleura. | in this review, three aspects of pleural disease are discussed. although it was thought for many years that the origin of pleural fluid was the capillaries in the parietal or visceral pleura, recent evidence suggests that in many cases the origin of pleural fluid is the interstitial space of the lung. the interstitial space of the lung appears to be the source of the pleural fluid in patients who have congestive heart failure, parapneumonic effusions, pulmonary embolism, and lung transplants. th ... | 1995 | 9363070 |
| new pathogens for respiratory infections. | the emergence of new pathogens and the increasing antimicrobial resistance patterns of old pathogens are contributing factors to the high epidemiologic impact of lower respiratory tract infections. hantaviruses, chlamydia pneumoniae, and penicillin-resistant streptococcus pneumoniae have recently gained most of the attention of international researchers. hantavirus pulmonary syndrome has been confirmed in 100 cases with a very high mortality (52%). risk factors for infection are peridomestic cle ... | 1996 | 9363136 |
| cell culture adaptation of puumala hantavirus changes the infectivity for its natural reservoir, clethrionomys glareolus, and leads to accumulation of mutants with altered genomic rna s segment. | this paper reports the establishment of a model for hantavirus host adaptation. wild-type (wt) (bank vole-passaged) and vero e6 cell-cultured variants of puumala virus strain kazan were analyzed for their virologic and genetic properties. the wt variant was well adapted for reproduction in bank voles but not in cell culture, while the vero e6 strains replicated to much higher efficiency in cell culture but did not reproducibly infect bank voles. comparison of the consensus sequences of the respe ... | 1997 | 9371614 |
| laguna negra virus associated with hps in western paraguay and bolivia. | a large outbreak of hantavirus pulmonary syndrome (hps) recently occurred in the chaco region of paraguay. using pcr approaches, partial virus genome sequences were obtained from 5 human sera, and spleens from 5 calomys laucha rodents from the outbreak area. genetic analysis revealed a newly discovered hantavirus, laguna negra (ln) virus, to be associated with the hps outbreak and established a direct genetic link between the virus detected in the hps cases and in the c. laucha rodents, implicat ... | 1997 | 9375015 |
| pleural fluid characteristics in hantavirus pulmonary syndrome. | hantavirus pulmonary syndrome (hps), is a rodent-borne, acute, often fulminant cardiorespiratory illness. noncardiogenic pulmonary edema is prominent in hps as is cardiac dysfunction. pleural effusions are commonly noted in patients with hps and have been thought to be exudative. this report describes the prevalence and characteristics of pleural effusions by an assessment of chest radiographs for the presence of pleural fluid and reviews all pleural fluid specimens obtained from patients with h ... | 1997 | 9377934 |
| [epidemic hantavirus nephropathy]. | | 1997 | 9381000 |
| sequence analysis of the puumala hantavirus sotkamo strain l segment. | sequence of the puumala virus (puu) sotkamo strain l segment is provided, completing the total genome of this prototype puu virus strain. the l segment is 6530 nucleotides long and it can encode 2156 amino-acids-long l protein, rna-dependent rna polymerase. the strain sotkamo, originally isolated in finland, showed for the l genome segment nucleotide (84.6%) and amino acid (97.3%) homology to a previously sequenced puu russian isolate, strain bashkiria/cg1820 (b1820) and the l genome segment app ... | 1997 | 9381791 |
| a small-scale survey of hantavirus in mammals from indiana. | in order to determine if hantaviruses were present in mice and other small mammals in indiana (usa), small mammals were trapped in brown, laporte, tippecanoe and whitley counties. sixty-seven small mammals were trapped during august and september 1994. sixty-three peromyscus leucopus, one microtus pennsylvanicus, one zapus hudsonius and two blarina brevicauda were captured and tested for hantaviruses. six p. leucopus were found to have antibody to sin nombre virus (sn) by igg elisa, and a 139 bp ... | 1997 | 9391967 |
| evaluation of serological methods for diagnosis of puumala hantavirus infection (nephropathia epidemica). | nephropathia epidemica (ne), puumala (puu) virus infection, is a febrile disease which is commonly associated with acute renal impairment. to differentiate ne from other acute febrile illnesses, a rapid and reliable serological diagnosis is important, and a number of different protocols have recently been introduced. in the present report we describe a comparative evaluation of six puu virus immunoglobulin m (igm) and seven igg enzyme-linked immunosorbent assay (elisa) protocols based on native, ... | 1997 | 9399531 |
| hantavirus pulmonary syndrome: cd8+ and cd4+ cytotoxic t lymphocytes to epitopes on sin nombre virus nucleocapsid protein isolated during acute illness. | in 1993 a number of cases of unexplained adult respiratory syndrome occurred in the southwestern united states. the illness was characterized by a prodrome of fever, myalgia, and other symptoms followed by the rapid onset of a capillary leak syndrome with hemoconcentration, thrombocytopenia, and pulmonary edema. viral rna sequences in the lungs identified a new member of the hantavirus genus, sin nombre virus (snv), unique to north america. pulmonary endothelial cells were heavily infected but w ... | 1997 | 9400611 |
| impaired pulmonary function in patients with hemorrhagic fever with renal syndrome. | pulmonary and cardiac functions were investigated in 13 patients hospitalized with nephropathia epidemica, a european form of hemorrhagic fever with renal syndrome. as compared with reference values, the patients' diffusion capacity for carbon monoxide was decreased (p = .002) and pulmonary clearance of inhaled technetium-99m-labeled diethylenetriamine pentaacetic acid was increased (p = .002). in four of 11 patients, arterial blood gas analysis disclosed a reduction in partial pressure of o2 (< ... | 1997 | 9402362 |
| [serologic studies of domestic cats for potential human pathogenic virus infections from wild rodents]. | for several viral infections a reservoir in wild rodents has been demonstrated. some of the agents are known or suspected to be pathogenic for humans. because improvements in hygiene have reduced direct human contact with rodents, domestic cats could be acting as active transmitters of these viruses from rodents to man. we selected 4 such pathogens--ortho- and parapox-, hanta- and encephalomyocarditis viruses--which, in different ways, may lead to serious human illness: ortho- and parapoxvirus i ... | 1996 | 9409901 |
| the investigation of emerging and re-emerging viral diseases: a paradign. | emerging virus infections are defined as previously nonthreatening viruses that can decimate new populations by finding fresh hosts and vectors--often with the help of humans who introduce new species into virgen environment, several etiologic agents of these diseases, some of the interacting factors that contribute to their development and the role of molecular medicine in their understanding is discussed. | 1997 | 9419931 |
| genetic analysis of the diversity and origin of hantaviruses in peromyscus leucopus mice in north america. | nucleotide sequences were determined for the complete m genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and pcr-positive white-footed mice (peromyscus leucopus) from indiana and oklahoma. phylogenetic analyses indicated that although divergent from each other, the virus lineages in indiana and oklahoma were monophyletic and formed a newly identified unique ancestral branch within the clade of sin nombre-like viruses found in peromyscus mice ... | 1998 | 9420200 |
| severe thrombocytopenia and response to corticosteroids in a case of nephropathia epidemica. | nine days after working in the woods, a previously healthy 32-year-old man fell seriously ill. his symptoms included high fever, chills, diffuse myalgia, severe headache, and back pain. on the fifth day of onset of symptoms, blood tests showed creatinine levels of 5.4 mg/dl accompanied by marked proteinuria. after admission to the hospital, a diagnosis of nephropathia epidemica (ne) caused by puumala virus was made using solid-phase enzyme-linked immunosorbent assay (elisa). the patient graduall ... | 1998 | 9428461 |
| hantavirus pulmonary syndrome in a chilean patient with recent travel in bolivia. | a case of hantavirus pulmonary syndrome (hps) was serologically confirmed in a critically ill patient in santiago, chile. the patient's clinical course had many similarities to that of other hps patients in north and south america but was complicated by acute severe renal failure. the patient's history included self-reported urban and probable rural rodent exposure during travel in bolivia. comparison of a viral sequence from an acute-phase serum sample with other known hantaviruses showed that ... | 1998 | 9452401 |
| bayou virus-associated hantavirus pulmonary syndrome in eastern texas: identification of the rice rat, oryzomys palustris, as reservoir host. | we describe the third known case of hantavirus pulmonary syndrome (hps) due to bayou virus, from jefferson county, texas. by using molecular epidemiologic methods, we show that rice rats (oryzomys palustris) are frequently infected with bayou virus and that viral rna sequences from hps patients are similar to those from nearby rice rats. bayou virus is associated with o. palustris; this rodent appears to be its predominant reservoir host. | 1998 | 9452404 |
| hantavirus infection in children in argentina. | clinical hantavirus infection was diagnosed in five argentine children ages 5 to 11 years by immunoglobulin m (igm)- capture enzyme-linked immunosorbent assay using sin nombre virus (snv) antigens. death in three of the children was associated with absence of detectable igg to snv antigens. an additional two cases in healthy children were studied: one, a breast-fed 15-month-old whose mother died of suspected hantavirus pulmonary syndrome (hps) 8 months previously, had hantavirus igg (> 1:6400); ... | 1998 | 9454562 |
| oligoryzomys flavescens (rodentia, muridae): gene flow among populations from central-eastern argentina. | in species acting as hosts of infectious agents, the extent of gene flow between populations is of particular interest because the expansion of different infectious diseases is usually related to the dispersal of the host. we have estimated levels of gene flow among populations of the sigmodontine rodent oligoryzomys flavescens, in which high titers of antibodies have been detected for a hantavirus in argentina that produces a severe pulmonary syndrome. enzyme polymorphism was studied by means o ... | 1997 | 9465403 |
| severe hantavirus pulmonary syndrome: a new indication for extracorporeal life support? | | 1998 | 9468156 |
| successful treatment of adults with severe hantavirus pulmonary syndrome with extracorporeal membrane oxygenation. | to describe our experience with the use of extracorporeal membrane oxygenation (ecmo) as a rescue therapy in adult patients with severe cardiopulmonary failure from hantavirus pulmonary syndrome. | 1998 | 9468181 |
| small mammal survival and trapability in mark-recapture monitoring programs for hantavirus. | following the 1993 hantavirus pulmonary syndrome (hps) epidemic in the south-western united states, mammalogists and epidemiologists instituted long-term studies to monitor population density and prevalence of infection in rodents which constitute the reservoir for sin nombre virus (snv). in this study, field techniques used in sampling small mammals for snv infection were evaluated to determine if trapping and handling protocols were having significant effects on future trapability or mortality ... | 1998 | 9476220 |
| [emerging infectious diseases--hantavirus infections]. | | 1997 | 9480304 |
| surveillance of anti-hantavirus antibodies among certain high-risk groups in taiwan. | hemorrhagic fever with renal syndrome is a serious health concern in neighboring countries of taiwan, such as mainland china and korea. in taiwan, only two suspected cases were recorded before 1994. the first confirmed case was reported in 1995, but this proved to be imported. to study hantavirus infection in taiwan, we tested blood collected from garbage collectors, animal handlers, patients with febrile illness of unknown origin, and field rats, the host of hantavirus, for the presence of anti ... | 1998 | 9481070 |
| hantavirus pulmonary syndrome. | | 1998 | 9493883 |
| viral hemorrhagic fevers and hantavirus infections in the americas. | several arenaviruses and hantaviruses have been isolated in the americas during the last 4 decades. these are rodent-borne viruses responsible for the south american hemorrhagic fevers (sahf) and hantavirus pulmonary syndrome (hps). although rare, sahf and hps are serious illnesses with high mortality rates. most viral isolates found in the americas represent new world lineages of their respective viral families. their presence in the western hemisphere is likely ancient, their relationship with ... | 1998 | 9494832 |
| emerging infectious diseases and travel medicine. | international movement of individuals, populations, and products is one of the major factors associated with the emergence and reemergence of infectious diseases as the pace of global travel and commerce increases rapidly. travel can be associated with disease emergence because (1) the disease arises in an area of heavy tourism, (2) tourists may be at heightened risk because of their activities, or (3) because they can act as vectors to transport the agent to new areas. examples of recently reco ... | 1998 | 9494841 |
| the enigmatic haemorrhagic fevers. | | 1997 | 9496275 |
| genetic diversity and epidemiology of hantaviruses in argentina. | phylogenetic analysis of a 292-nucleotide (nt) fragment of the hantavirus m genome segment from 36 rodent and 13 human samples from three known foci of hantavirus infection in argentina was conducted. a 1654-nt fragment of the m genome segment was analyzed for 1 representative of 7 genetically distinct hantavirus lineages identified. additionally, the nt sequence of the complete m genome segments of lechiguanas, oran, and hu39694 hantavirus genotypes was determined. nt sequence comparisons revea ... | 1998 | 9498428 |
| isolation, characterization and geographic distribution of caño delgadito virus, a newly discovered south american hantavirus (family bunyaviridae). | rodents collected from the venezuelan llanos (plains) during field studies of viral hemorrhagic fever were tested for evidence of hantavirus infection. hantavirus antibody was found in one (7.7%) of 13 oryzomys bicolor, one (3.4%) of 29 rattus rattus, 10 (6.0%) of 166 sigmodon alstoni and one (2.2%) of 45 zygodontomys brevicauda. hantavirus-specific rna was detected in lung tissues from four antibody-positive rodents: two s. alstoni from portuguesa state and one s. alstoni each from cojedes and ... | 1997 | 9498614 |
| hantavirus pulmonary syndrome outbreak in argentina: molecular evidence for person-to-person transmission of andes virus. | an increase of hantavirus pulmonary syndrome (hps) cases around a southwestern argentina town and in persons living 1400 km away but in contact with those cases was detected during the spring of 1996. in order to evaluate person-to-person transmission we compared the homology of pcr-amplified viral sequences of 26 argentine and chilean cases. sixteen of them were epidemiologically linked cases and had the same sequence (epilink/96) in the s segment 3' noncoding region and in the m segment partia ... | 1998 | 9499807 |
| genetic reassortment among viruses causing hantavirus pulmonary syndrome. | in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ... | 1998 | 9501041 |
| urine-associated horizontal transmission of seoul virus among rats. | to understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with the ki-83-262 strain of seoul virus, s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ... | 1998 | 9505963 |
| a miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers. | in this paper, we describe a miniature analytical thermal cycling instrument (matci) to amplify and detect dna via the polymerase chain reaction in real-time. the matci is an integrated, miniaturized analytical system that uses silicon-based, high-efficiency reaction chambers with integrated heaters and simple, inexpensive electronics to precisely control the reaction temperatures. optical windows in the silicon and solid-state, diode-based detection components are employed to perform real-time ... | 1998 | 9511467 |
| hantavirus pulmonary syndrome: implications for critical care nurses. | | 1998 | 9515465 |
| association of hla b27 with benign clinical course of nephropathia epidemica caused by puumala hantavirus. | both the severe course of nephropathia epidemica (ne) caused by puumala hantavirus, and the fast progression of human immunodeficiency virus (hiv) disease, are associated with the hla b8 drb1*0301 haplotype. as hla b27, on the contrary, is associated with the slow progression of hiv disease, we wanted to test whether the same is true for ne. only six (8%) ne patients, half the figure expected, had the hla b27 allele in 74 randomly selected hospital-treated patients. all six had a benign overall ... | 1998 | 9519867 |
| hantavirus infection. | | 1998 | 9520902 |
| role of actin microfilaments in black creek canal virus morphogenesis. | we have investigated the involvement of cytoskeletal proteins in the morphogenesis of black creek canal virus (bccv), a new world hantavirus. immunofluorescent staining of bccv-infected cells revealed a filamentous pattern of virus antigen, the appearance of which was sensitive to treatment with cytochalasin d, an actin microfilament-depolymerizing drug. double immunofluorescence staining of bccv-infected vero cells with anti-bccv nucleocapsid (n) monoclonal antibody and phalloidin revealed a co ... | 1998 | 9525606 |
| modes of seoul virus infections: persistency in newborn rats and transiency in adult rats. | to understand the mode of persistent infection of seoul virus in rodents, we examined the distribution of the virus genome and antibody production in infected rats. when 1-day-old rats were inoculated with the ki-83-262 strain, the s segment of viral genome was detected in sera, clots, lungs and kidneys from 3 to 184 days post inoculation (d.p.i.) by nested reverse transcriptase pcr. on the other hand, when 7-week-old rats were infected with this virus, viral genome was detected only in the lung ... | 1996 | 9526540 |
| neuropsychological impairments following hantavirus pulmonary syndrome. | recently an outbreak of acute respiratory infection associated with the hantavirus occurred in the southwestern united states. hantavirus pulmonary syndrome (hps) is a life threatening illness that carries with it a high mortality rate. patients with hps experience prolonged periods of hypoxemia requiring mechanical ventilation and treatment in intensive care units. we have recently seen 2 survivors of hps. a neuropsychological test battery was administered immediately following their acute hosp ... | 1998 | 9529829 |
| serologic survey for hantavirus infection in domestic animals and coyotes from new mexico and northeastern arizona. | to determine whether animals had serologic evidence of infection with sin nombre virus (snv). | 1998 | 9540866 |
| urine-associated horizontal transmission of seoul virus among rats. | to understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with ki-83-262 strain of seoul virus, the s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ... | 1998 | 9541619 |
| of mice and mostly men--hantavirus pulmonary syndrome. | | 1998 | 9559018 |
| human recombinant puumala virus antibodies: cross-reaction with other hantaviruses and use in diagnostics. | a panel of seven human monoclonal fabs against puumala virus (puu) nucleocapsid protein (n) was obtained by panning an antibody phage-display library prepared from the spleen of a puu-immune individual. three antibodies reacted in immunoblotting and cross-reacted strongly with tula and sin nombre virus recombinant n proteins. these antibodies mapped to the amino terminus of the n protein. one puu glycoprotein 2 (g2)-specific fab obtained against a novel epitope (g2c) cross-reacted with khabarovs ... | 1998 | 9568958 |
| central nervous system and ophthalmic involvement in nephropathia epidemica (european type of haemorrhagic fever with renal syndrome). | central nervous system (cns) - related symptoms occur in haemorrhagic fever with renal syndrome (hfrs). to study the cns and ophthalmic involvement in nephropathia epidemica (ne), the european type of hfrs, we included 26 patients in a prospective study. most common cns-related symptoms were headache (96%), insomnia (83%), vertigo (79%), nausea (79%), and vomiting (71%). ophthalmic symptoms were reported by 82% of patients; 41% had photophobia and 50% had impaired vision. a transient loss of vis ... | 1998 | 9570645 |
| a survey of hantavirus antibody in small-mammal populations in selected united states national parks. | hantavirus activity in 39 national parks in the eastern and central united states was surveyed by testing 1,815 small mammals of 38 species for antibody reactive to sin nombre virus. antibody-positive rodents were found throughout the area sampled, and in most biotic communities. antibody was detected in 7% of 647 deer mice (peromyscus maniculatus), 2% of 590 white-footed mice (p. leucopus), 17% of 12 rice rats (oryzomys palustris), 3% of 31 cotton rats (sigmodon hispidus), and 33% of 18 western ... | 1998 | 9574803 |
| hantavirus infection: a possible cause of delayed sensorineural hearing loss. | | 1998 | 9591876 |
| emerging pathogens: the hong kong experience. | | 1997 | 9594216 |
| [serologic verification of epidemic hemorrhagic fever during the lifting of the blockade in sarajevo]. | after four years of sarajevo siege, the deblocade started on july 1995. many soldiers involved in the deblocade developed a clinical symptoms of hemorrhagic fever indicating a possible epidemic. | 1996 | 9601761 |
| adjuvant activity of muramyl dipeptide derivatives to enhance immunogenicity of a hantavirus-inactivated vaccine. | the adjuvant effect of two lipophilic derivatives of muramyl dipeptide (mdp), b30-mdp and mdp-lys(l18), on the ability of an inactivated vaccine of b-1 virus (b-1 vaccine) to induce immune response against hantavirus causing hemorrhagic fever with renal syndrome (hfrs) was examined. when mice were immunized subcutaneously (s.c.) twice at 2-week intervals with b-1 vaccine admixed with or without 100 micrograms mouse-1 of b30-mdp (b-1/b30-mdp) or mdp-lys(l18) [b-1/mdp-lys(l18)], mice immunized wit ... | 1998 | 9607033 |
| chimaeric hbv core particles carrying a defined segment of puumala hantavirus nucleocapsid protein evoke protective immunity in an animal model. | hantaviruses are rodent-born agents which are pathogenic in humans causing haemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. to induce a protective immunity against a european hantavirus (puumala) we constructed chimaeric hepatitis b virus (hbv) core particles carrying defined fragments of the puumala virus nucleocapsid protein. after immunisation of bank voles, the natural host of puumala virus, with core particles possessing an insertion of the n-terminal part of puumala ... | 1998 | 9607042 |
| tissue distribution of hantavirus antigen in naturally infected humans and deer mice. | the sin nombre virus (snv) is the etiologic agent of hantavirus pulmonary syndrome in humans but does not cause disease in chronically infected deer mice (peromyscus maniculatus), the natural host. in this study, murine antiserum raised against recombinant snv nucleocapsid protein was utilized to localize viral antigen immunohistochemically in tissues from both humans (n = 20; 11 positive, 9 negative) and deer mice (n = 6; 4 positive, 2 negative). viral infection status was confirmed by western ... | 1998 | 9607851 |
| haemorrhagic fever with renal syndrome caused by puumala virus: evaluation of the risk for cataract formation. | | 1998 | 9614533 |
| glomerular basement membrane antibodies in hantavirus disease: any pathogenetic implication? | | 1998 | 9617503 |
| beta3 integrins mediate the cellular entry of hantaviruses that cause respiratory failure. | newly emerged hantaviruses replicate primarily in the pulmonary endothelium, cause acute platelet loss, and result in hantavirus pulmonary syndrome (hps). we now report that specific integrins expressed on platelets and endothelial cells permit the cellular entry of hps-associated hantaviruses. infection with hps-associated hantaviruses, ny-1 and sin nombre virus (snv), is inhibited by antibodies to beta3 integrins and by the beta3-integrin ligand, vitronectin. in contrast, infection with the no ... | 1998 | 9618541 |
| prevalence of antibodies specific to puumala virus among farmers in sweden. | the puumala virus is the causative agent of nephropathia epidemica, a european form of hemorrhagic fever with a renal syndrome. from its reservoir in bank voles, the virus is spread by airborne transmission to humans. occupational risks for the acquisition of nephropathia epidemica are not well defined. the prevalence of serum antibodies to puumala virus was determined for swedish farmers. from a comparison of the prevalence among farmers from various parts of the country, the assumption that pu ... | 1998 | 9630057 |
| hantavirus pulmonary syndrome--colorado and new mexico, 1998. | hantavirus pulmonary syndrome (hps) is a severe cardiopulmonary illness resulting in death in approximately 45% of cases. the most frequently recognized etiologic agent of hps in north america, sin nombre virus (snv), is transmitted to humans from its primary rodent reservoir, peromyscus maniculatus (deer mouse), by direct contact with infected rodents, rodent droppings, or nests or through inhalation of aerosolized virus particles from mouse urine and feces. sporadic cases occur throughout the ... | 1998 | 9639364 |
| hantavirus pulmonary syndrome in the province of rio negro, argentina, 1993-1996. | early in 1995 the first case of hantavirus pulmonary syndrome was serologically confirmed in el bolsón (province of río negro, argentina), corresponding to the third outbreak reported in argentina. a total of 26 cases of hps related to the andean region of rio negro province, were reported from 1993 to 1996, 17 in el bolsón, 4 in san carlos de bariloche, and 5 in buenos aires. the incidence rate was 5.03 x 100,000 with a mortality rate of 51.85 x 100. the occurrence of cases was mainly seasonal, ... | 1997 | 9640780 |
| hantavirus pulmonary syndrome. report of the first three cases in são paulo, brazil. | the hantavirus pulmonary syndrome was first recognized in cases that occurred in the u.s. in 1993, which served as an alert not only for american physicians but also for physicians in other countries for the identification of the disease. in the city of são paulo, brazil, 3 cases of the syndrome were recorded in 1993. the patients were young brothers residing in the mata atlântica (atlantic forest) region submitted to recent deforestation. two of the patients died of acute respiratory insufficie ... | 1997 | 9640788 |
| hantavirus pulmonary syndrome in the rural area of juquitiba, são paulo metropolitan area, brazil. | | 1997 | 9640790 |
| evaluation of puumala virus igg and igm enzyme immunoassays based on recombinant baculovirus-expressed nucleocapsid protein for early nephropathia epidemica diagnosis. | puumala virus (puu), a member of hantavirus genus, is the causative agent of nephropathia epidemica (ne), a milder form of hemorrhagic fever with renal syndrome (hfrs). rapid diagnosis is essential for clinical management of ne. | 1998 | 9646005 |
| [the pulmonary syndrome produced by hantaviruses]. | | 1997 | 9654969 |
| short report: decrease in seroprevalence of antibodies to hantavirus in rodents from 1993-1994 hantavirus pulmonary syndrome case sites. | rodent trapping was conducted at seven hantavirus pulmonary syndrome (hps) case sites from june 1993 to march 1994 during the hps outbreak in the southwestern united states. to determine if there were changes in the rodent population or the hantavirus seroprevalence in rodents since the hps outbreak, rodents were trapped at the same sites three years later using the same trapping protocol. the trap success decreased from the numbers trapped during the outbreak, however, the number of peromyscus, ... | 1998 | 9660455 |
| retrospective serological and genetic study of the distribution of hantaviruses in greece. | a retrospective serological and genetic study of hantaviruses responsible for hemorrhagic fever with renal syndrome (hfrs) in greece during the last 17 years is presented. fifty-one serum samples taken from 30 hfrs cases previously diagnosed by immunofluorescence assay were tested by elisa for igg (hantaan, dobrava, and puumala) and igm antibodies (hantaan and puumala). results were compatible with the majority of infections being related to hantaviruses carried by rodents of the subfamily murin ... | 1998 | 9661842 |
| acute exacerbation of autoimmune liver disease associated with hantaviral infection. | hantavirus is known to cause haemorrhagic fever with renal syndrome (hfrs). although liver dysfunction has been reported in hfrs, hepatic manifestations of hantaviral infection have not been well described. we describe a case of autoimmune cholangitis in which an exacerbation of hepatitis was associated with hantaviral infection. seroconversion of both igg- and igm-class antibodies to hantavirus was noted coincident with acute exacerbation of hepatitis, which was resolved promptly by treatment w ... | 1998 | 9670364 |
| distinguishing hantavirus pulmonary syndrome from acute respiratory distress syndrome by chest radiography: are there different radiographic manifestations of increased alveolar permeability? | hantavirus infection may cause diffuse air space disease, termed hantavirus pulmonary syndrome (hps). the authors sought to determine if chest radiographs could differentiate hps from typical acute respiratory distress syndrome (ards). the authors identified patients with either hps (n = 11) or acute ards (n = 32) and selected the earliest chest radiograph showing diffuse airspace disease, and a chest radiograph taken 24 to 48 hours previously. thoracic and general radiologists first viewed the ... | 1998 | 9671418 |
| [the history of the identification of pulmonary syndrome due to hantavirus in the argentina southern andes]. | | 1997 | 9674235 |
| [hantavirus emergence in the americas and in argentina]. | | 1998 | 9674240 |
| [pathogenesis, physiopathology, clinical manifestations and treatment of hantavirus pulmonary syndrome]. | | 1998 | 9674241 |
| [epidemiology of hantavirus pulmonary syndrome in argentina (1991-1997)]. | | 1998 | 9674242 |
| [epidemic outbreak of hantavirus pulmonary syndrome in argentina. molecular evidence of person to person transmission of andes virus]. | | 1998 | 9674243 |
| [reservoirs and genetic viral diversity]. | | 1998 | 9674244 |
| [hantavirus pulmonary syndrome. summary of the findings in argentina]. | | 1998 | 9674245 |
| [aids and the challenge of the emergent diseases. closing remarks]. | | 1998 | 9674258 |
| pathogenesis of a north american hantavirus, black creek canal virus, in experimentally infected sigmodon hispidus. | this report describes the first detailed analysis of the replication, persistence, and excretion of a north american hantavirus in its natural rodent reservoir. black creek canal virus was isolated from sigmodon hispidus (cotton rat) shortly after the identification of a hantavirus pulmonary syndrome (hps) case occurring in southern florida. six-week-old male cotton rats were inoculated subcutaneously with 1,000 tissue culture infectious doses. viral complementary rna (vcrna) was quantified as a ... | 1998 | 9684629 |
| automatic detection of conserved rna structure elements in complete rna virus genomes. | we propose a new method for detecting conserved rna secondary structures in a family of related rna sequences. our method is based on a combination of thermodynamic structure prediction and phylogenetic comparison. in contrast to purely phylogenetic methods, our algorithm can be used for small data sets of approximately 10 sequences, efficiently exploiting the information contained in the sequence variability. the procedure constructs a prediction only for those parts of sequences that are consi ... | 1998 | 9685502 |
| characterization of the nucleic acid binding properties of tomato spotted wilt virus nucleocapsid protein. | tomato spotted wilt tospovirus (tswv) is the type member of the plant-infecting viruses of the genus tospovirus in the family bunyaviridae. the three tswv rnas are encapsidated with nucleocapsid (n) protein to form ribonucleoprotein (rnp) which serves as the template for viral transcription and replication. regions of the open reading frame coding for the n protein on the small (s) rna were subcloned into pet protein expression vectors and expressed in escherichia coli bl21 (de3) cells. full-len ... | 1998 | 9705250 |
| antigenic characterization of hantaan and seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen. | hantaan virus (htn) and seoul virus (seo) are members of the genus hantavirus in the family bunyaviridae and are causative agents of hemorrhagic fever with renal syndrome. the complete and truncated nucleocapsid proteins (np) of htn and seo were expressed by a recombinant baculovirus system. antigenic characterization of the np using monoclonal antibodies (mabs) indicated that the binding sites for the serotype-specific mabs were located between amino acids (aa) 155 and 429. a western blot assay ... | 1998 | 9705385 |
| from the centers for disease control and prevention. hantavirus pulmonary syndrome--colorado and new mexico, 1998. | | 1998 | 9707130 |
| southwest cautious over hantavirus outbreak. | | 1998 | 9713524 |
| host genes and infectious diseases. | | 1998 | 9716963 |
| human dobrava hantavirus infections in estonia. | outbreaks of severe hemorrhagic fever with renal syndrome (hfrs) caused by dobrava hantavirus (dob) have been reported in albania, greece, and bosnia-herzegovina. a seroepidemiologic study conducted by the authors in estonia provided the first evidence of human dob infection in a baltic country. hantavirus infection was first confirmed in estonia in 1986 and believed related to puumala hantavirus (puu). to enable precise serotyping, serum samples were collected from 1728 healthy people in 11 ... | 1998 | 9717928 |
| [america hantaviruses: new data and implications]. | | 1998 | 9718552 |
| isolated abducens nerve palsy as a complication of haemorrhagic fever with renal syndrome. | | 1998 | 9719177 |
| hantavirus pulmonary syndrome treated with inhaled nitric oxide. | | 1998 | 9726354 |
| lack of serologic evidence for an association between hantavirus infection and end-stage renal disease. | | 1998 | 9730717 |
| zoonoses and haemorrhagic fever. | virus zoonoses causing haemorrhagic fever have been recognized in three major families: arenaviridae, bunyaviridae and filoviridae. all are negative-stranded rna viruses, with genomes in two segments, three segments, or non-segmented, respectively. acquisition of haemorrhagic fever in man generally requires close contact with a vertebrate vector species, usually rodents, for the arenaviruses and bunyaviruses. in the case of filoviruses, the vector is currently unknown, but these viruses may infe ... | 1998 | 9737374 |
| hantaviruses: an emerging threat to human health? | | 1998 | 9742991 |
| high prevalence of hantavirus infection in indian communities of the paraguayan and argentinean gran chaco. | serologic evidence of past infection with a sin nombre-like hantavirus(es) was demonstrated in 78 (40.4%) of 193 indians living in western paraguay and in 38 (17.1%) of 222 indians inhabiting the salta province of northern argentina. in both populations seroprevalence increased with age, with the most striking increase occurring at 18 years of age in the paraguayan population and at 35 years of age in the salta population. the peak prevalences in both populations (66.6% and 44.0%, respectively) ... | 1998 | 9749641 |