Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| [hantavirosis: clinical and epidemiological characteristics of pediatric patients in chile]. | the first cases of hantavirus cardiopulmonary syndrome in children were described in the united states and represented 8% of the total reported cases; in chile, the proportion of pediatric cases represents 15% of all national cases. | 2010 | 20140316 |
| case-control study on puumala virus infection: smoking is a risk factor. | puumala hantavirus (puuv) is apparently transmitted to humans by inhalation of aerosolized secretions of carrier rodents (bank voles). the means of transmission and the associated risk factors are poorly defined. an epidemiological study during the peak of an epidemic season in finland was conducted based on 282 acute clinical puuv infections and 204 controls without puuv infection or immunity. the main risk factors adjusted by age, sex and living environment were cigarette smoking [odds ratio ( ... | 2010 | 19781114 |
| hantavirus transmission in sylvan and peridomestic environments. | we developed a compartmental model for hantavirus infection in deer mice (peromyscus maniculatus) with the goal of comparing relative importance of direct and indirect transmission in sylvan and peridomestic environments. a direct transmission occurs when the infection is mediated by the contact of an infected and an uninfected mouse, while an indirect transmission occurs when the infection is mediated by the contact of an uninfected mouse with, for instance, infected soil. based on population d ... | 2010 | 19821001 |
| hantavirus nephropathy as a pseudo-import pathology from ecuador. | we report a case of hantavirus infection (nephropathia epidemica) diagnosed in a belgian backpacker returning from a trekking expedition in ecuador, after likely heavy exposure to rodents. because of epidemiological inconsistency, molecular investigation was performed and revealed a puumala infection acquired during very limited exposure in belgium upon return. | 2010 | 19821128 |
| interactions and oligomerization of hantavirus glycoproteins. | in this report the basis for the structural architecture of the envelope of hantaviruses, family bunyaviridae, is systematically studied by the interactions of two glycoproteins n and c (gn and gc, respectively) and their respective disulfide bridge-mediated homo- and heteromeric oligomerizations. in virion extracts gn and gc associated in both homo- and hetero-oligomers which were, at least partially, thiol bridge mediated. due to strong homo-oligomerization, the hetero-oligomers of gn and gc a ... | 2010 | 19828613 |
| pathogenic hantaviruses direct the adherence of quiescent platelets to infected endothelial cells. | hantavirus infections are noted for their ability to infect endothelial cells, cause acute thrombocytopenia, and trigger 2 vascular-permeability-based diseases. however, hantavirus infections are not lytic, and the mechanisms by which hantaviruses cause capillary permeability and thrombocytopenia are only partially understood. the role of beta(3) integrins in hemostasis and the inactivation of beta(3) integrin receptors by pathogenic hantaviruses suggest the involvement of hantaviruses in altere ... | 2010 | 20181715 |
| extensive host sharing of central european tula virus. | to examine the host association of tula virus (tulv), a hantavirus present in large parts of europe, we investigated a total of 791 rodents representing 469 microtus arvalis and 322 microtus agrestis animals from northeast, northwest, and southeast germany, including geographical regions with sympatric occurrence of both vole species, for the presence of tulv infections. based on serological investigation, reverse transcriptase pcr, and subsequent sequence analysis of partial small (s) and mediu ... | 2010 | 19889769 |
| study of andes virus entry and neutralization using a pseudovirion system. | andes virus (andv), a member of the hantavirus genus in the family bunyaviridae, causes an acute disease characteristic of new-world hantaviruses called hantavirus pulmonary syndrome (hps). hps is a highly pathogenic disease with a case-fatality rate of 40%. andv is the only hantavirus reported to spread directly from human-to-human. the aim of the present study was to develop a quantitative and high-throughput pseudovirion assay to study andv infection and neutralization in biosafety level 2 fa ... | 2010 | 19903496 |
| one-step rna pathogen detection with reverse transcriptase activity of a mutated thermostable thermus aquaticus dna polymerase. | we describe the cloning and characterization of a mutated thermostable dna polymerase from thermus aquaticus (taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step pcr pathogen detection using established real-time detection methods. we demonstrate that this taq polymerase mutant (taq m1) has similar pcr sensitivity and nuclease activity as the respective taq wild-type dna polymerase. in addition, and in marked contrast to the wild-type, taq m1 e ... | 2010 | 20108275 |
| production and characterization of a recombinant single-chain antibody against hantaan virus envelop glycoprotein. | hantaan virus (htnv) is the type of hantavirus causing hemorrhagic fever with renal syndrome, for which no specific therapeutics are available so far. cell type-specific internalizing antibodies can be used to deliver therapeutics intracellularly to target cell and thus, have potential application in anti-htnv infection. to achieve intracellular delivery of therapeutics, it is necessary to obtain antibodies that demonstrate sufficient cell type-specific binding, internalizing, and desired cellul ... | 2010 | 20012277 |
| reappearance of viral hemorrhagic fever with renal syndrome in northwestern greece. | minor outbreaks of viral hemorrhagic fever (vhf) with renal failure have occurred in northwestern greece over past decades. however, during the most recent decade, there has been a paucity of human cases despite the detection of hantavirus in rodents of this area. | 2010 | 19926507 |
| genetic detection of dobrava/belgrade virus in a czech patient with haemorrhagic fever with renal syndrome. | in the summer of 2008, a 15-year-old boy was hospitalized in a paediatric intensive care unit in the czech republic. laboratory diagnosis of hantavirus infection was established by serological and molecular methods. sequence and phylogenetic analyses showed that the causative strain was dobrava/belgrade virus, which is genetically closer to strains associated with apodemus flavicollis rodents. | 2010 | 19832712 |
| environmental and ecological potential for enzootic cycles of puumala hantavirus in great britain. | puumala virus (puuv) is a zoonotic rodent-borne hantavirus in continental europe. its reservoir host, the bank vole (myodes glareolus), is ubiquitous in great britain (gb); however, there has been no reported incidence of virus in either animals or humans. in northwest europe, increases in bank vole numbers, stimulated by increases in production of beech/oak crops (mast), are associated with outbreaks of nephropathia epidemica (ne) in humans. these so-called 'mast years' are determined by sequen ... | 2010 | 19563697 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20104956 |
| ecology of hantavirus in a changing world. | hantavirus is a genus of virus represented by 45 different species and is hosted by small mammals, predominantly rats and mice. roughly, half of all hantaviruses cause diseases in humans that vary in morbidity from mild to severe. the natural and anthropogenic changes occurring in the environment appear to be impacting the ecology of hantaviruses and their natural hosts as well as the incidence of hantaviral diseases in humans. although such studies are limited at this time, there is evidence th ... | 2010 | 20536819 |
| [hantavirus infection associated to a staphylococcal sepsis. report of one case]. | we report a previously healthy 36-year-old mole who developed a pulmonary syndrome caused by hantavirus infection. he was admitted to an intensive care unit in shock and respiratory failure and died four hours after admission. blood cultures were positive for staphylococcus aureus. this patient could be an index case that shows, for the first time, an association of hantavirus pulmonary syndrome with staphylococcus aureus infection similar to what happens with other viruses. | 2010 | 20556338 |
| new world hantaviruses activate ifnlambda production in type i ifn-deficient vero e6 cells. | hantaviruses indigenous to the new world are the etiologic agents of hantavirus cardiopulmonary syndrome (hcps). these viruses induce a strong interferon-stimulated gene (isg) response in human endothelial cells. african green monkey-derived vero e6 cells are used to propagate hantaviruses as well as many other viruses. the utility of the vero e6 cell line for virus production is thought to owe to their lack of genes encoding type i interferons (ifn), rendering them unable to mount an efficient ... | 2010 | 20567522 |
| [construction and identification of a new type of recombinant adenovirus containing s0.7 gene of hantavirus]. | to construct a adenovirus vector containing the 0.7 kb fragment of s gene of hantavirus, cag promoter, and wpre (mrna-stabilizing post-transcriptional regulatory element from the woodchuck hepatitis virus). | 2010 | 20423645 |
| genetic analysis of a hantavirus strain carried by niviventer confucianus in yunnan province, china. | hantavirus genome sequences were recovered from lung tissues of chinese white-bellied rats (niviventer confucianus) captured in yunnan province, china. pairwise comparison of the nucleotide and deduced amino acid sequences of the entire s and partial m and l segments indicated that the newly discovered virus strain, which was designated as strain yn509, was very different from other rodent-borne hantaviruses. phylogenetic analysis showed that the new strain fit into a clade containing da bie sha ... | 2010 | 20600393 |
| [dynamics of hantavirus detection in the excretory organs of apodemus mice and its relation to epidemic manifestations of hantavirus infection]. | this paper presents data on the detection of hantavirus in the excretory organs of apodemus mice which are natural carriers of pathogenic hantaviral serotypes in the primorye region. acute infection periods were detected in naturally infected rodents with the highest hantavirus dissemination to the environment in different phases of rodent population cycles. there was a relationship between the rise of morbidity and the seasonal manifestation of hemorrhagic fever with renal syndrome morbidity an ... | 2010 | 20455471 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20462286 |
| interaction of hantavirus nucleocapsid protein with ribosomal protein s19. | hantaviruses, members of the bunyaviridae family, are emerging category a pathogens that initiate the translation of their capped mrnas by a novel mechanism mediated by viral nucleocapsid protein (n). n specifically binds to the mrna 5' m7g cap and 40s ribosomal subunit, a complex of 18s rrna and multiple ribosomal proteins. here, we show that n specifically interacts with the ribosomal protein s19 (rps19), located at the head region of the 40s subunit. we suggest that this n-rps19 interaction f ... | 2010 | 20844026 |
| pathogenic hantaviruses andes virus and hantaan virus induce adherens junction disassembly by directing vascular endothelial cadherin internalization in human endothelial cells. | hantaviruses infect endothelial cells and cause 2 vascular permeability-based diseases. pathogenic hantaviruses enhance the permeability of endothelial cells in response to vascular endothelial growth factor (vegf). however, the mechanism by which hantaviruses hyperpermeabilize endothelial cells has not been defined. the paracellular permeability of endothelial cells is uniquely determined by the homophilic assembly of vascular endothelial cadherin (ve-cadherin) within adherens junctions, which ... | 2010 | 20463083 |
| the severity of acute puumala hantavirus infection does not predict the long-term outcome of patients. | we have found greater urinary protein excretion and higher glomerular filtration rate (gfr) and blood pressure in patients 6 years after acute nephropathia epidemica (ne) compared with seronegative controls. the present aim was to establish whether the long-term outcome is determined by the severity of acute illness. | 2010 | 20502044 |
| extinction of refugia of hantavirus infection in a spatially heterogeneous environment. | we predict an abrupt observable transition, on the basis of numerical studies, of hantavirus infection in terrain characterized by spatially dependent environmental resources. the underlying framework of the analysis is that of fisher equations with an internal degree of freedom, the state of infection. the unexpected prediction is of the sudden disappearance of refugia of infection in spite of the existence of supercritical (favorable) food resources, brought about by reduction of their spatial ... | 2010 | 20866661 |
| generation and characterization of genetic reassortants between puumala and prospect hill hantavirus in vitro. | hantaviruses belong to the family bunyaviridae characterized by tri-segmented rna genomes. depending on the hantavirus species, infection can lead to hantavirus cardiopulmonary or haemorrhagic fever with renal syndrome. in vitro studies suggest that pathogenic hantaviruses evade induction of innate antiviral responses, and this ability might determine the virulence in humans. since reverse genetic systems are not available, in vitro reassortment is currently the only way to culture defined hanta ... | 2010 | 20505009 |
| early diagnosis of hantavirus infection by family doctors can reduce inappropriate antibiotic use and hospitalization. | hantavirus infections are emerging infections that cause either hantavirus pulmonary syndrome or haemorrhagic fever with renal syndrome (hfrs). a recent swedish outbreak of nephropathia epidemica, a european hfrs, was analysed to study the patient flow and clinical picture and to investigate the value of an early diagnosis in general practice. design. in a retrospective design, medical records of verified cases of hantavirus infection were studied. | 2010 | 20642397 |
| central nervous system-related symptoms and findings are common in acute puumala hantavirus infection. | puumala hantavirus (puuv) causes a hemorrhagic fever with renal syndrome (hfrs) also called nephropathia epidemica (ne). recent case reports and retrospective studies suggest that ne may damage the pituitary gland. based on these observations, our goal was to explore the nature of this complication prospectively. | 2010 | 20545485 |
| [hantavirus]. | 2010 | 20942073 | |
| hantavirus infections in fluctuating host populations: the role of maternal antibodies. | infected females may transfer maternal antibodies (matabs) to their offspring, which may then be transiently protected against infections the mother has encountered. however, the role of maternal protection in infectious disease dynamics in wildlife has largely been neglected. here, we investigate the effects of puumala hantavirus (puuv)-specific matabs on puuv dynamics, using 7 years' data from a cyclic bank vole population in finland. for the first time to our knowledge, we partition seroposit ... | 2010 | 20591866 |
| occupation-related respiratory infections revisited. | occupational pulmonary infectious diseases include tuberculosis (tb) and many viral pathogens, including influenza, coronavirus (severe acute respiratory syndrome or sars), varicella, respiratory syncytial virus, and hantavirus. this review focuses on tb, influenza, and sars, because the published literature is extensive for these 3 infections. the lessons from these 3 are relevant for all nosocomial pulmonary infectious diseases. | 2010 | 20674797 |
| in vivo kinetics and biodistribution of a hantaan virus dna vaccine after intramuscular injection in mice. | to study the kinetics in vivo of a hantaan virus dna vaccine, we constructed a fusion dna vaccine, pegfp/s, by cloning the s segment of hantavirus into the vector, pegfp-c1, which encodes green fluorescent protein egfp. in this report, we provide evidence that pegfp/s was distributed and persistently expressed for more than 60 days in several organs after inoculation. our findings suggest that the persistent immune responses induced by a hantaan virus dna vaccine are likely due to the plasmid pe ... | 2010 | 20960291 |
| pathogenicity and virulence of the present hantaviruses in bosnia and herzegovina: the impact on renal function. | dobrava (dobv) and puumala (puuv) viruses are endemic throughout the balkans and cause haemorrhagic fever with renal syndrome (hfrs). the aim of this study was to assess the impact of two different hantaviruses on renal function in hfrs patients during the acute stage of illness. we also aimed to assess the dobv and puuv distribution between symptomatic hfrs patients and asymptomatic hantavirus antibody-positive subjects. the study included 264 symptomatic hfrs patients and 63 asymptomatic hanta ... | 2010 | 20972691 |
| hantaviruses. | hantaviruses affect people worldwide, yet they remain poorly understood. this article explores the known history of hantaviruses. it describes diagnostic methods and potential options for treatment and prevention. | 2010 | 20513542 |
| sin nombre virus-specific immunoglobulin m and g kinetics in hantavirus pulmonary syndrome and the role played by serologic responses in predicting disease outcome. | sin nombre virus (snv) is the primary cause of hantavirus pulmonary syndrome (hps) in the united states. although other studies have demonstrated a possible association between neutralizing antibody titers and the severity of hps, the exact nature of serologic responses and their association with outcomes have not been fully characterized. | 2010 | 20521946 |
| a global perspective on hantavirus ecology, epidemiology, and disease. | hantaviruses are enzootic viruses that maintain persistent infections in their rodent hosts without apparent disease symptoms. the spillover of these viruses to humans can lead to one of two serious illnesses, hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome. in recent years, there has been an improved understanding of the epidemiology, pathogenesis, and natural history of these viruses following an increase in the number of outbreaks in the americas. in this review, curre ... | 2010 | 20375360 |
| tnf-α expression and promoter sequences reflect the balance of tolerance/resistance to puumala hantavirus infection in european bank vole populations. | the tumor necrosis factor-alpha (tnf-α) influences the ability to limit parasite infection but its over-production might result in inflammatory disorders. the level of tnf-α gene expression could thus mediate a balance of tolerance/resistance to infections. this study focused on puumala hantavirus (puuv) infection in its rodent host, the bank vole (myodes glareolus). in humans, puuv is responsible of a mild form of hemorrhagic fever with renal syndrome, nephropathia epidemica (ne). the severity ... | 2010 | 20691810 |
| relationship of human behavior within outbuildings to potential exposure to sin nombre virus in western montana. | sin nombre virus (snv) causes hantavirus cardiopulmonary syndrome (hcps) in humans. transmission of snv among the deer mouse (peromyscus maniculatus) host predominates during spring and summer, and is greater in peridomestic than sylvan settings where, protected from uv light, snv may survive longer. incidence of hcps reflects these times and settings and is associated with inhalation of mouse excreta. little is known, however, about how human use of outbuildings contributes to potential exposur ... | 2010 | 20508970 |
| convalescent pulmonary dysfunction following hantavirus pulmonary syndrome in panama and the united states. | the objective of this study was to document persistent pulmonary symptoms and pulmonary function abnormalities in adults surviving hantavirus pulmonary syndrome (hps). acute infection by most hantaviruses result in mortality rates of 25-35%, while in panama the mortality rate of 10% is contrasted by an unusually high incidence. in all types of hps, the viral prodrome, cardiopulmonary phase due to massive pulmonary capillary leak syndrome, and spontaneous diuresis are followed by a convalescent p ... | 2010 | 20524006 |
| travellers and viral haemorrhagic fevers: what are the risks? | viral haemorrhagic fevers (vhf) are caused by zoonotic viral infections transmitted to humans directly or by ticks or mosquitoes. the overall risk to travellers is conservatively estimated at <1 in 1 million travel episodes to african countries where infection is present, and febrile patients returning from these countries are at least 1000 times more likely to have malaria than lassa fever or another vhf. no cases have been reported in fellow travellers exposed to a travelling case and only one ... | 2010 | 20705436 |
| pathology of black creek canal virus infection in juvenile hispid cotton rats (sigmodon hispidus). | the purpose of this study was to assess the effect of inoculum dose on the pathogenesis of black creek canal virus (bccv) infection in the hispid cotton rat (sigmodon hispidus), the principal host of bccv. no sign of illness was observed in any of the 52 juvenile hispid cotton rats inoculated with 3.1, 1.1, -0.9, or -2.9 log(10) median infectious doses(veroe6) (id(50-veroe6)) of bccv and euthanized on day 9, 18, 27, or 54 postinoculation (pi). analysis of virus assay and serological data indicat ... | 2010 | 20455779 |
| seoul virus suppresses nf-kappab-mediated inflammatory responses of antigen presenting cells from norway rats. | hantavirus infection reduces antiviral defenses, increases regulatory responses, and causes persistent infection in rodent hosts. to address whether hantaviruses alter the maturation and functional activity of antigen presenting cells (apcs), rat bone marrow-derived dendritic cells (bmdcs) and macrophages (bmdms) were generated and infected with seoul virus (seov) or stimulated with tlr ligands. seov infected both dcs and macrophages, but copies of viral rna, viral antigen, and infectious virus ... | 2010 | 20170933 |
| hantavirus pulmonary syndrome. | hantavirus pulmonary syndrome, also known as hantavirus cardiopulmonary syndrome, is a recently described infectious syndrome found throughout the americas. although infection is sporadic and uncommon compared with other atypical pneumonia syndromes, its high mortality rate warrants the maintenance of a high index of suspicion in rural settings. because no specific therapies are available for the disease, prevention and early recognition play an important role in reducing mortality from the dise ... | 2010 | 20171551 |
| a case report of crescentic glomerulonephritis associated with hantaan virus infection. | although various glomerular diseases in hantavirus infection have been reported, an association between hantavirus infection and crescentic glomerulonephritis has not been described. herein, we report a case of immune complex-mediated crescentic glomerulonephritis in a 70-year-old man with hantaan virus infection. | 2010 | 20466689 |
| comparison of the effects of puumala and dobrava viruses on early and long-term renal outcomes in patients with haemorrhagic fever with renal syndrome. | the clinical course and outcome of patients with haemorrhagic fever with renal syndrome (hfrs) caused by puumala (puuv) and dobrava viruses (dobv) were analyzed and whether it left long-term consequences on kidney function after 10 years was evaluated. | 2010 | 20470304 |
| the severity of puumala hantavirus induced nephropathia epidemica can be better evaluated using plasma interleukin-6 than c-reactive protein determinations. | nephropathia epidemica (ne) is a scandinavian type of hemorrhagic fever with renal syndrome caused by puumala hantavirus. the clinical course of the disease varies greatly in severity. the aim of the present study was to evaluate whether plasma c-reactive protein (crp) and interleukin (il)-6 levels associate with the severity of ne. | 2010 | 20500875 |
| highly differentiated, resting gn-specific memory cd8+ t cells persist years after infection by andes hantavirus. | in man, infection with south american andes virus (andv) causes hantavirus cardiopulmonary syndrome (hcps). hcps due to andv is endemic in southern chile and much of argentina and increasing numbers of cases are reported all over south america. a case-fatality rate of about 36% together with the absence of successful antiviral therapies urge the development of a vaccine. although t-cell responses were shown to be critically involved in immunity to hantaviruses in mouse models, no data are availa ... | 2010 | 20174562 |
| steep rise in notified hantavirus infections in germany, april 2010. | from january to april 2010, 396 hantavirus infections were notified in germany, a considerable increase compared with previous years (mean: 83 for january-april 2004-2009) including the record-setting year, 2007 (n=232 january-april). most patients are residents of known puumala virus endemic areas in southern germany. the recent increase in notified hantavirus infections is probably due to an increased population density of the main animal reservoir, the bank vole (myodes glareolus). | 2010 | 20504391 |
| electron cryotomography of tula hantavirus suggests a unique assembly paradigm for enveloped viruses. | hantaviruses (family bunyaviridae) are rodent-borne emerging viruses that cause a serious, worldwide threat to human health. hantavirus diseases include hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. virions are enveloped and contain a tripartite single-stranded negative-sense rna genome. two types of glycoproteins, g(n) and g(c), are embedded in the viral membrane and form protrusions, or "spikes." the membrane encloses a ribonucleoprotein core, which consists of ... | 2010 | 20219926 |
| characterization and outcome following puumala virus infection: a retrospective analysis of 75 cases. | infection with the puumala virus (puuv), which belongs to the hantavirus family, is a common but often neglected cause of acute kidney injury (aki) in endemic areas of europe. the objective of the present study was to systematically analyse clinical presentation and renal outcomes following puuv infection. | 2010 | 20223893 |
| modulation of apoptosis and immune signaling pathways by the hantaan virus nucleocapsid protein. | herein, we show a direct relationship between the hantaan virus (htnv) nucleocapsid (n) protein and the modulation of apoptosis. we observed an increase in caspase-7 and -8, but not -9 in cells expressing htnv n protein mutants lacking amino acids 270-330. similar results were observed for the new world hantavirus, andes virus. nuclear factor kappa b (nf-kappab) was sequestered in the cytoplasm after tumor necrosis factor receptor (tnfr) stimulation in cells expressing htnv n protein. further, t ... | 2010 | 20227103 |
| interaction between hantaviral nucleocapsid protein and the cytoplasmic tail of surface glycoprotein gn. | hantaviral n and gn proteins were shown to interact, thus providing the long-awaited evidence for one of the crucial steps in the virus replication at which rnps are directed to the site of the virus assembly. using pull-down assay and point mutagenesis it was demonstrated that intact, properly folded zinc fingers in the gn protein cytoplasmic tail as well as the middle domain of the n protein (that includes aa residues 80-248) are essential for the interaction. | 2010 | 20566401 |
| beechnuts and outbreaks of nephropathia epidemica (ne): of mast, mice and men. | 2010 | 20237057 | |
| the triplet repeats of the sin nombre hantavirus 5' untranslated region are sufficient in cis for nucleocapsid-mediated translation initiation. | hantavirus nucleocapsid protein (n) can replace the cellular cap-binding complex, eukaryotic initiation factor 4f (eif4f), to mediate translation initiation. although n can augment translation initiation of nonviral mrna, initiation of viral mrna by n is superior. all members of the bunyaviridae family, including the species of the hantavirus genus, express either three or four primary mrnas from their tripartite negative-sense genomes. the 5' ends of the mrnas contain nonviral heterologous olig ... | 2010 | 20573811 |
| enhanced thrombin formation and fibrinolysis during acute puumala hantavirus infection. | nephropathia epidemica (ne) is a viral hemorrhagic fever with renal syndrome associated with thrombocytopenia and mild bleeding. we assessed activation of coagulation and fibrinolysis during the acute phase of ne. | 2010 | 20579693 |
| serological evidence of human hantavirus infections in guinea, west africa. | we recently discovered a novel hantavirus, sangassou virus, in guinea, west africa. using enzyme-linked immunosorbent assays followed by confirmatory and serotyping assays, we retrospectively detected hantavirus antibodies in 3 (4.4%) of 68 patients with fever of unknown origin in sangassou village, forest guinea. a population-based survey in forest guinea (n = 649) found the prevalence of hantavirus antibodies to be 1.2%. specific neutralizing antibodies against sangassou virus were demonstrate ... | 2010 | 20187741 |
| generation of e3-deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences. | seoul virus (seov) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. the medium genome segment encodes the gn/gc glycoproteins of seov, which form the major structural part of the virus envelope. gc and/or gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. in this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gc protein of seov w ... | 2010 | 20181748 |
| association of -308g/a polymorphism in the tumor necrosis factor-alpha gene promoter with susceptibility to development of hantavirus cardiopulmonary syndrome in the ribeirão preto region, brazil. | activation of the immune response in hantavirus cardiopulmonary syndrome (hcps) leads to a high tnf production, probably contributing to the disease. the polymorphic tnf2 allele (tnf -308g/a) has been associated with increased cytokine production. we investigated the association of the tnf2 allele with the outcome of hantavirus infection in brazilian patients. a total of 122 hantavirus-exposed individuals (26 presenting hcps and 96 only hantavirus seroconversion) were studied. the tnf2 allele wa ... | 2010 | 20372945 |
| hantavirus outbreak associated with laboratory rats in yunnan, china. | an outbreak of hemorrhagic fever with renal syndrome occurred among students in a college (college a) in kunming, yunnan province, china in 2003. subsequent investigations revealed the presence of hantavirus antibodies and antigens in laboratory rats at college a and two other institutions. hantavirus antibodies were detected in 15 additional individuals other than the index case in these three locations. epidemiologic data indicated that the human infections were a result of zoonotic transmissi ... | 2010 | 20380897 |
| roles of human disturbance, precipitation, and a pathogen on the survival and reproductive probabilities of deer mice. | climate change, human disturbance, and disease can have large impacts on the dynamics of a species by affecting the likelihood of survival and reproduction of individuals. we investigated the roles of precipitation, off-road vehicle (orv) alteration of habitat, and infection with sin nombre virus on the survival and reproductive probabilities of deer mice (peromyscus maniculatus). we used generalized linear mixed models to estimate the effects of these factors and their interactions by fitting c ... | 2010 | 20392022 |
| hormonal deficiencies during and after puumala hantavirus infection. | previous reports have described panhypopituitarism associated with severe cases of hemorrhagic fever with renal syndrome (hfrs), but the prevalence of hormonal deficiencies after nephropathia epidemica (ne), a milder form of hfrs, has not been studied. this study was conducted in order to determine the prevalence of hormonal defects in patients with acute ne and during long-term follow-up. fifty-four patients with serologically confirmed acute ne were examined by serum hormonal measurements duri ... | 2010 | 20397036 |
| temporal and spatial host abundance and prevalence of andes hantavirus in southern argentina. | andes virus (and) is a hantavirus hosted by the sigmodontine rodent oligoryzomys longicaudatus in southern argentina, where it is responsible for most cases of hantavirus pulmonary syndrome (hps). our study provides data about the spatial variation in abundance of the rodent host of and hantavirus. we report results of a longitudinal study performed in a locality of the andean region of chubut province. from november 2003 (spring) to july 2006 (winter), o. longicaudatus was the most common speci ... | 2010 | 20645121 |
| confirmation of choclo virus as the cause of hantavirus cardiopulmonary syndrome and high serum antibody prevalence in panama. | choclo virus (chov) was described in sigmodontine rodents, oligoryzomys fulvescens, and humans during an outbreak of hantavirus cardiopulmonary syndrome (hcps) in 1999-2000 in western panama. although hcps is rare, hantavirus-specific serum antibody prevalence among the general population is high suggesting that chov may cause many mild or asymptomatic infections. the goals of this study were to confirm the role of chov in hcps and in the frequently detected serum antibody and to establish the p ... | 2010 | 20648614 |
| hantaviruses in small mammals and humans in the coastal region of zhejiang province, china. | to gain more insights into the epidemiology of hantaviruses in the coastal region of zhejiang province, china, the morbidity and mortality of hemorrhagic fever with renal syndrome (hfrs) were analyzed in two coastal areas: cixi (hilly terrain) and wenzhou (mountainous terrain). more hfrs cases have been reported in cixi than in wenzhou. annual incidence rate of hfrs in cixi had been on the level of approximately 1.5/100,000 from 1968 (when the first hfrs case was reported) to 2007, with the high ... | 2010 | 20419812 |
| high-resolution computed tomography findings in hantavirus pulmonary syndrome. | the hantavirus pulmonary syndrome (hps) is an acute, rapidly progressive disease transmitted by rodent excreta, with endothelial damage playing a central role in the pathophysiology. it usually affects rural workers. the lung itself is the target organ and reflects all the patterns of endothelial involvement of this disease. the radiologic findings of hps are vast and range from a mild interstitial involvement to total obliteration of the airspaces with or without pleural effusion. there are no ... | 2010 | 20351581 |
| comprehensive panel of real-time taqman polymerase chain reaction assays for detection and absolute quantification of filoviruses, arenaviruses, and new world hantaviruses. | viral hemorrhagic fever is caused by a diverse group of single-stranded, negative-sense or positive-sense rna viruses belonging to the families filoviridae (ebola and marburg), arenaviridae (lassa, junin, machupo, sabia, and guanarito), and bunyaviridae (hantavirus). disease characteristics in these families mark each with the potential to be used as a biological threat agent. because other diseases have similar clinical symptoms, specific laboratory diagnostic tests are necessary to provide the ... | 2010 | 20439981 |
| hantavirus infections in humans and animals, china. | hemorrhagic fever with renal syndrome (hfrs) is a serious public health problem in the people's republic of china. although 7 sero/genotypes of hantaviruses have been found in rodents, only hantaan virus (carried by apodemus agrarius mice) and seoul virus (carried by rattus norvegicus rats) reportedly cause disease in humans. during 1950-2007, a total of 1,557,622 cases of hfrs in humans and 46,427 deaths (3%) were reported in china. hfrs has been reported in 29 of 31 provinces in china. after i ... | 2010 | 20678311 |
| cytoplasmic tails of hantavirus glycoproteins interact with the nucleocapsid protein. | here we characterize the interaction between the glycoproteins (gn and gc) and the ribonucleoprotein (rnp) of puumala virus (puuv; genus hantavirus, family bunyaviridae). the interaction was initially established with native proteins by co-immunoprecipitating puuv nucleocapsid (n) protein with the glycoprotein complex. mapping of the interaction sites revealed that the n protein has multiple binding sites in the cytoplasmic tail (ct) of gn and is also able to bind to the predicted ct of gc. the ... | 2010 | 20444994 |
| tula virus in populations of small terrestrial mammals in a rural landscape. | over 5 years (2000-2004), populations of small mammals from a rural landscape in southern moravia (czech republic) were investigated for the presence of tula virus (tulv) antigen using the elisa set hantagnost. in total, 1566 individuals from 10 species were examined. the prevalence in the common vole (microtus arvalis pallas 1778), the main reservoir of tulv, was 10% (n = 871). the prevalence of tulv antigen increases with its population numbers. the highest number of tulv antigen-positive comm ... | 2010 | 20420534 |
| infection of in vivo differentiated human mast cells with hantaviruses. | increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ... | 2010 | 20071485 |
| novel hantavirus in the flat-skulled shrew (sorex roboratus). | genetically distinct hantaviruses have been identified recently in multiple species of shrews (order soricomorpha, family soricidae) in eurasia and north america. to corroborate decades-old reports of hantaviral antigens in shrews from russia, archival liver and lung tissues from 4 siberian large-toothed shrews (sorex daphaenodon), 5 eurasian least shrews (sorex minutissimus), 12 flat-skulled shrews (sorex roboratus), and 18 tundra shrews (sorex tundrensis), captured in the sakha republic in nor ... | 2010 | 20426682 |
| seewis virus: phylogeography of a shrew-borne hantavirus in siberia, russia. | hantaviral antigens were originally reported more than 20 years ago in tissues of the eurasian common shrew (sorex araneus), captured in european and siberian russia. the recent discovery of seewis virus (swsv) in this soricid species in switzerland provided an opportunity to investigate its genetic diversity and geographic distribution in russia. | 2010 | 20426688 |
| hantavirus-like particles generated in cho cells induce specific immune responses in c57bl/6 mice. | a safe and effective hantavirus vaccine is highly desirable since hantaviruses are distributed worldwide and cause an acute and often fatal disease (hemorrhagic fever with renal syndrome, hfrs). virus-like particles (vlps) displaying functional viral proteins could provide effective vaccines against a few viruses, but their ability to induce hantavirus-specific immune response has not been adequately investigated. to measure the immunogenicity of hantaan virus-like particles (htn-vlps) vaccine, ... | 2010 | 20433802 |
| tula hantavirus nss protein accumulates in the perinuclear area in infected and transfected cells. | the small rna segment of some hantaviruses (family bunyaviridae) encodes two proteins: the nucleocapsid protein and, in an overlapping reading frame, a non-structural (nss) protein. the hantavirus nss protein, like those of orthobunya- and phleboviruses, counteracts host innate immunity. here, for the first time, the nss protein of a hantavirus (tula virus) has been observed in infected cells and shown to localize in the perinuclear area. transiently expressed nss protein showed similar localiza ... | 2010 | 19956987 |
| truncated hantavirus nucleocapsid proteins for serotyping sin nombre, andes, and laguna negra hantavirus infections in humans and rodents. | sin nombre virus (snv), andes virus (andv), and laguna negra virus (lanv) have been known as the dominant causative agents of hantavirus pulmonary syndrome (hps). andv and lanv, with different patterns of pathogenicity, exist in a sympatric relationship. moreover, there is documented evidence of person-to-person transmission of andv. therefore, it is important in clinical medicine and epidemiology to know the serotype of a hantavirus causing infection. truncated snv, andv, and lanv recombinant n ... | 2010 | 20335425 |
| hantavirus pulmonary syndrome, french guiana. | 2010 | 20350412 | |
| recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: toward the development of high-throughput screening flow cytometry assays. | hantaviruses cause two severe diseases in humans: hemorrhagic fever with renal syndrome (hfrs) and hantavirus cardiopulmonary syndrome (hcps). the lack of vaccines or specific drugs to prevent or treat hfrs and hcps and the requirement for conducting experiments in a biosafety level 3 laboratory (bsl-3) limit the ability to probe the mechanism of infection and disease pathogenesis. in this study, we developed a generalizable spectroscopic assay to quantify saturable fluorophore sites solubilized ... | 2010 | 20363206 |
| the effect of seasonality, density and climate on the population dynamics of montana deer mice, important reservoir hosts for sin nombre hantavirus. | 1. since sin nombre virus was discovered in the u.s. in 1993, longitudinal studies of the rodent reservoir host, the deer mouse (peromyscus maniculatus) have demonstrated a qualitative correlation among mouse population dynamics and risk of hantavirus pulmonary syndrome (hps) in humans, indicating the importance of understanding deer mouse population dynamics for evaluating risk of hps. 2. using capture-mark-recapture statistical methods on a 15-year data set from montana, we estimated deer mous ... | 2010 | 20015212 |
| puumala hantavirus and myodes glareolus in northern europe: no evidence of co-divergence between genetic lineages of virus and host. | the genus hantavirus (family bunyaviridae) includes negative-strand rna viruses that are carried by persistently infected rodent and insectivore species. puumala virus (puuv), carried by bank voles (myodes glareolus), is a pathogenic hantavirus that causes outbreaks of mild haemorrhagic fever with renal syndrome across europe. in northern europe, puuv is represented by several genetic lineages that are maintained by distinct phylogroups of bank voles. the present study describes sequences of new ... | 2010 | 20107019 |
| sin nombre virus infection in field workers, colorado, usa. | we report 2 cases of sin nombre virus (snv) infection in field workers, possibly contracted through rodent bites. screening for antibodies to snv in rodents trapped in 2 seasons showed that 9.77% were seropositive. quantitative real-time pcr showed that 2 of 79 deer mice had detectable titers of snv rna. | 2010 | 20113567 |
| different cross-reactivity of human and rodent sera to tula virus and puumala virus. | tula virus (tulv) and puumala virus (puuv) are hantaviruses carried by the bank vole (myodes glareolus) and european common vole (microtus arvalis), respectively. puuv is a causative agent of hemorrhagic fever with renal syndrome (hfrs), while tulv is thought to be apathogenic to humans. the n-terminal regions of the n proteins from tulv and puuv were expressed and applied as enzyme-linked immunosorbent assay (elisa) antigens. colonized japanese grass voles (microtus montebelli) and balb/c mice ... | 2010 | 20116854 |
| hantavirus nucleocapsid protein has distinct m7g cap- and rna-binding sites. | hantaviruses, members of the bunyaviridae family, are emerging category a pathogens that carry three negative stranded rna molecules as their genome. hantavirus nucleocapsid protein (n) is encoded by the smallest s segment genomic rna (viral rna). n specifically binds mrna caps and requires four nucleotides adjacent to the cap for high affinity binding. we show that the n peptide has distinct cap- and rna-binding sites that independently interact with mrna cap and viral genomic rna, respectively ... | 2010 | 20164193 |
| serologic survey of hantavirus infection, brazilian amazon. | 2010 | 20409399 | |
| hantaviruses and hantavirus pulmonary syndrome, maranhao, brazil. | to confirm circulation of anajatuba virus in maranhao, brazil, we conducted a serologic survey (immunoglobulin g elisa) and phylogenetic studies (nucleocapsid gene sequences) of hantaviruses from wild rodents and persons with hantavirus pulmonary syndrome. this virus is transmitted by oligoryzomys fornesi rodents and is responsible for hantavirus pulmonary syndrome in this region. | 2010 | 21122229 |
| [genetic analysis of hantaviruses carried by rattus norvegicus collected from henan and neimenggu provinces, china.] | objective: genetic analysis was performed to infer the relationship between hantaviruses carried by rattus norvegicus from henan and neimenggu provinces and the other known hantavirus and the vaccine strain. methods: total rna was extracted from lung tissues with trizol reagent. the complete m and s segment sequences of strains nm133 and q12 were amplified by rt-pcr. the purified dna fragments were directly subjected to sequencing, and then to sequence analysis and phylogenetic analysis. results ... | 2010 | 21162871 |
| [epidemiology and surveillance programs on hemorrhagic fever with renal syndrome in mainland china, 2005 - 2008.] | objective: to analyze the epidemiologic and surveillance data of hemorrhagic fever with renal syndrome (hfrs) in china, from 2005 to 2008, to describe the epidemiology and trend of hfrs. methods: descriptive epidemiology were studied to analyze the surveillance data from 2005 to 2008, collected from both the internet-based national notifiable disease reporting system and 40 hfrs sentinel sites developed since 2005 in 40 counties around china. results: a total of 56 077 hfrs cases and 692 deaths ... | 2010 | 21163102 |
| an efficient in vivo method for the isolation of puumala virus in syrian hamsters and the characterization of the isolates from russia. | puumala virus (puuv) and other arvicolinae-borne hantaviruses are difficult to cultivate in cell culture. to isolate these hantaviruses efficiently, hantavirus nucleocapsid protein (np)-positive but seronegative wild rodents were selected by np-detection elisa. three of 68 myodes glareolus captured in samara, russia, were np-positive and seronegative. syrian hamsters were inoculated with lung homogenates from np-positive rodents for virus propagation. virus isolation in vitro was carried out by ... | 2010 | 21192975 |
| [nephropathia epidemica caused by puumala hantavirus infection: a case report from the outpatient sector]. | a 42-year-old man without pre-existing health problems presented to his family practitioner in baden-württemberg with fever (up to 39˚ c for two days), headache, abdominal pain, back pain and aching limbs. ten days before, he had done heavy gardening work. he felt diffuse pain on abdominal palpation. the physical examination of the skin, lymph nodes, the heart, the lungs and the oral mucosa was unremarkable. | 2010 | 21140328 |
| aromatic and polar residues spanning the candidate fusion peptide of the andes virus gc protein are essential for membrane fusion and infection. | hantaviruses infect human cells through cell attachment and subsequent fusion of viral and cellular membranes at low ph. this largely unknown entry process is mediated by the gn and gc glycoproteins, anchored at the viral envelope membrane. performing bioinformatic analysis and peptide-liposome-binding assays we suggested in a former report that gc of andes virus (andv) and other hantaviruses corresponds to the viral fusion protein sharing characteristics with class ii fusion proteins. to gain i ... | 2010 | 21123541 |
| temporal variation in individual factors associated with hantavirus infection in bank voles during an epizootic: implications for puumala virus transmission dynamics. | abstract puumala virus (puuv), the causal agent of nephropathia epidemica in humans, is one of the many hantaviruses included in the list of emerging pathogens. hantavirus infection is not distributed evenly among puuv reservoir hosts (i.e., bank voles [myodes glareolus]). besides environmental factors and local population features, individual characteristics play an important role in vole puuv infection risk. identifying the relative importance of these individual characteristics can provide cr ... | 2010 | 21142469 |
| [hemorrhagic fever with renal syndrome during pregnancy: case report]. | hemorrhagic fever with renal syndrome (hfwrs) is an acute infectious disease with abrupt onset, high fever, renal failure and frequent hemorragies. hfwrs during the pregnancy has relatively low occurrence, with only few described cases mostly in the region of the former soviet union. although, according to the data from the literature, the disease is less severe during the pregnancy, the eclampsy, stillbirth and fetal infection were also described. | 2010 | 21053473 |
| characterization of two substrains of puumala virus that show phenotypes that are different from each other and from the original strain. | hantaviruses, the causative agents of two emerging diseases, are negative-stranded rna viruses with a tripartite genome. we isolated two substrains from a parental strain of puumala hantavirus (puuv-pa), puuv-small (puuv-sm) and puuv-large (puuv-la), named after their focus size when titrated. the two isolates were sequenced; this revealed differences at two positions in the nucleocapsid protein and two positions in the rna-dependent rna polymerase, but the glycoproteins were identical. we also ... | 2010 | 21106742 |
| the 3' untranslated region of the andes hantavirus small mrna functionally replaces the poly(a) tail and stimulates cap-dependent translation initiation from the viral mrna. | in the process of translation of eukaryotic mrnas, the 5' cap and the 3' poly(a) tail interact synergistically to stimulate protein synthesis. unlike its cellular counterparts, the small mrna (smrna) of andes hantavirus (andv), a member of the bunyaviridae, lacks a 3' poly(a) tail. here we report that the 3' untranslated region (3'utr) of the andv smrna functionally replaces a poly(a) tail and synergistically stimulates cap-dependent translation initiation from the viral mrna. stimulation of tra ... | 2010 | 20660206 |
| characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples. | monoclonal antibodies are important tools for various applications in hantavirus diagnostics. recently, we generated puumala virus (puuv)-reactive monoclonal antibodies (mabs) by immunisation of mice with chimeric polyomavirus-derived virus-like particles (vlps) harbouring the 120-amino-acid-long amino-terminal region of the puuv nucleocapsid (n) protein. here, we describe the generation of two mabs by co-immunisation of mice with hexahistidine-tagged full-length n proteins of sin nombre virus ( ... | 2010 | 21161552 |
| hantavirus pulmonary syndrome in argentina, 1995-2008. | we report a large case series of hantavirus pulmonary syndrome (hps) in argentina that was confirmed by laboratory results from 1995 through 2008. the geographic and temporal distribution of cases by age, sex, fatality rate, and risk factors for hps was analyzed. a total of 710 cases were unequally distributed among 4 of the 5 argentine regions. different case-fatality rates were observed for each affected region, with a maximum rate of 40.5%. the male-to-female ratio for hps case-patients was 3 ... | 2010 | 21122213 |
| rapid expansion and long-term persistence of elevated nk cell numbers in humans infected with hantavirus. | natural killer (nk) cells are known to mount a rapid response to several virus infections. in experimental models of acute viral infection, this response has been characterized by prompt nk cell activation and expansion followed by rapid contraction. in contrast to experimental model systems, much less is known about nk cell responses to acute viral infections in humans. we demonstrate that nk cells can rapidly expand and persist at highly elevated levels for >60 d after human hantavirus infecti ... | 2010 | 21173105 |
| a pauci-symptomatic case of documented hantavirus (puumala) infection in a patient under anti-tnf treatment. | we describe the case of an 18-yr-old male under anti-tnf treatment for crohn's disease for more than 8 months. he developed fever and biological inflammatory syndrome without absolutely no accompanying sign or symptom or paraclinical abnormality despite extensive work-up performed in the context of his immunocompromised state. symptoms disappeared after 10 days and a diagnosis of puumala infection was made retrospectively on a serological basis. the case illustrates that anti-tnf treatment does ... | 2010 | 21195021 |
| identification of a region of hantavirus nucleocapsid protein required for rna chaperone activity. | sin nombre hantavirus (snv) is a new world hantavirus and causes hantavirus cardiopulmonary syndrome. the viral nucleocapsid protein (n) is an rna chaperone and has multiple functions important in virus replication. the three negative sense rna segments of hantaviruses form panhandle structures through imperfect hydrogen bonding of the 5' and 3' termini, and the chaperone activity of n can mediate correct panhandle formation. n also functions during transcription and translation initiation and t ... | 2010 | 21378500 |
| evidence of human hantavirus infection and zoonotic investigation of hantavirus prevalence in rodents in western java, indonesia. | abstract during febrile surveillance in the western java city of bandung, indonesia, a patient with clinical symptoms consistent with hantavirus infection was found to have elevated titers of hantavirus-specific immunoglobulin m (igm) and igg antibodies. a subsequent epizoological investigation demonstrated a higher prevalence of hantavirus igg antibodies in rodents trapped in the vicinity of the patient's home compared with rodents from a control area (13.2% vs. 4.7%, p = 0.036). the old world ... | 2010 | 21142967 |
| small mammal-associated zoonoses. | 2010 | 20795915 |