| human recombinant neutralizing antibodies against hantaan virus g2 protein. | old world hantaviruses, causing hemorrhagic fever with renal syndrome (hfrs), still present a public health problem in asia and eastern europe. the majority of cases has been recorded in china. the aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. to preserve the structural identity of viral protein, the panning procedure was performed on native hantaan (htn) (76-118) virus propagated in vero-e6 cells. in total, five complete ... | 2003 | 12706090 |
| [hantavirus pulmonary syndrome: current situation among rodent reservoirs and human population in the 10th region, chile]. | in chile, three hantavirus seropositive rodent species (o longicaudatus, a olivaceus and a longipilis) are distributed from the pacific coast to the andes mountains and represent nearly 90% of the rodents captured in the xth region. | 2003 | 12708255 |
| [hantaviruses]. | hantaviruses are zoonotic diseases that affect humans and have a worldwide distribution. the hemorrhagic fever associated with renal syndrome occurs endemically in the asian and european continents affecting housauds of people every year. hantavirus cardiopulmonary syndrome, recognized as a clinical entity since 1993, represents the prototype of emerging diseases and is distributed in countries of the american continent, including brazil. both diseases are transmitted to man through the inhalati ... | 2003 | 12715067 |
| lymphocyte apoptosis during hemorragic fever with renal syndrome. | programmed cell death (pcd) is a fundamental process regulating immune homeostasis. during acute viral infections pcd of lymphocytes by apoptosis is necessary for removing the excess of activated antigen-reactive t cells and down-regulation of the immune response. on the other hand, apoptosis is the key mechanism of elimination of viral-infected cells. at the present time there is virtually no data on mechanisms of immune regulation during human hantavirus infections. in this study we examined t ... | 2003 | 12717553 |
| traveling waves of infection in the hantavirus epidemics. | traveling waves are analyzed in a model of the hantavirus infection in deer mice. the existence of two kinds of wave phenomena is predicted. an environmental parameter governs a transition between two regimes of propagation. in one of them the front of infection lags behind at a constant rate. in the other, fronts of susceptible and infected mice travel at the same speed, separated by a constant delay. the dependence of the delay on system parameters is analyzed numerically and through a piecewi ... | 2003 | 12749537 |
| [hantavirus]. | | 2003 | 12718024 |
| [hantavirus pulmonary syndrome]. | | 2003 | 12722234 |
| human tula virus infection or rat-bite fever? | | 2003 | 12736795 |
| design of oligonucleotide arrays to detect point mutations: molecular typing of antibiotic resistant strains of neisseria gonorrhoeae and hantavirus infected deer mice. | microarrays are promising tools for use in molecular diagnostics due to their ability to perform a multitude of tests simultaneously. in the case of genotyping many such tests will require discrimination of sequence at the single nucleotide level. a number of challenges exist including binding of optimal quantities of probe to the chip surface, the use of uniform hybridization conditions across the chip and the generation of labeled target. we investigated two model systems to test out the effic ... | 2003 | 12788028 |
| quantitation of antibody-bound and unbound sin nombre virus in the plasma of patient with hantavirus pulmonary syndrome. | hantavirus pulmonary syndrome (hps) is a rare but acute fulminant disease caused by sin nombre virus and other related hantaviruses. we reported earlier that hps patients had high levels of viremia and the levels of viremia correlated with the severity of the disease. to determine whether the viruses are free in plasma or bound to antibodies to form immune complexes, we established a method to quantitate viral genomic rna in antibody-bound viruses and in unbound viruses separately. to isolate im ... | 2003 | 12798243 |
| [the pathogenicity of hfrs patient's soft palate mucosa to suckling mice]. | to explore the pathogenic mechanism of hemorrhagic fever with renal syndrome(hfrs). | 1999 | 12759963 |
| evidence of the presence of seoul virus in cambodia. | a study was conducted in agricultural and urban areas in cambodia to assess the presence of hantaviruses in rodent populations. in 1998, rodents were trapped in two villages and in phnom penh city around market places and a rubbish dump. igg antibodies to hantaan virus were detected in 54 (8.2%) rodents among 660 tested: 6.4% (13/203) among roof rats (rattus rattus), 20.9% (39/187) among norway rats (r. norvegicus), 16.7% (2/12) among unidentified rattus species and none in 183 polynesian rats ( ... | 2003 | 12850202 |
| genetic interaction between dobrava and saaremaa hantaviruses: now or millions of years ago? | | 2003 | 12768038 |
| hantaviruses in the czech republic. | | 2003 | 12781027 |
| hantavirus infection in people inhabiting a highly endemic region of the gran chaco territory, paraguay: association with trypanosoma cruzi infection, epidemiological features and haematological characteristics. | the seroprevalences of anti-hantavirus antibodies were determined in 712 individuals (551 indians, 140 mennonites of german ancestry, and 21 paraguayans of spanish ancestry) inhabiting a region of western paraguay in the gran chaco territory of south america. the overall seroprevalence of hantavirus infection among the 712 subjects, who were aged 2-80 years, was 42.7% (45.2% in the indians and 34.2% in the non-indians). of the 672 subjects also checked for antibodies against trypanosoma cruzi, 2 ... | 2003 | 12803858 |
| hantaviruses induce cytopathic effects and apoptosis in continuous human embryonic kidney cells. | hantaviruses are maintained in nature in persistently infected rodents and can also persistently infect cultured mammalian cells, causing little or no cytopathology. an unexpected outcome of this study was the observation of cytopathic effects (cpe) in the hantavirus-infected human embryonic kidney cell line hek293. it was confirmed that hantaviruses induce apoptosis in hek293 cells, although apoptosis appeared mostly in uninfected, bystander cells and rarely in infected hek293 cells. although s ... | 2003 | 12867652 |
| [molecular characterization of hantavirus shandong isolate jnl virus strain]. | to understand the molecular epidemiologic characteristics of hantavirus shandong isolate jnl virus strain. | 2003 | 12869988 |
| [construction and stable expression of intracellular antibodies to glycoprotein of hantavirus]. | to understand the molecular mechanisms of hantavirus assembly and maturation by stably expressing the intracellular antibodies to hantavirus glycoprotein g1 and g2 in endoplasmic reticulum (er) and cytoplasm (cyto) of vero e6 cell. | 2003 | 12869989 |
| [molecular analysis of hantavirus isolated from shandong province]. | to compare the molecular characteristics of hantaviruses isolated from shandong province by using pcr typing and nucleotide sequencing. | 2003 | 12869990 |
| [serum antibody response and hantavirus rna sequencing in patients with hemorrhagic fever renal syndrome in yantai areas]. | to understand antibody responses to and rna sequences of hantavirus in patients with hemorrhagic fever renal syndrome (hfrs) in yantai areas and to demonstrate the type of the prevalent viruses caused hfrs. | 2003 | 12869991 |
| [study of hantavirus seoul in a human and rodent population from a marginal area in buenos aires city]. | a sero-epidemiological survey was conducted to detect evidence of the circulation of hantavirus seoul. this virus of worldwide distribution is associated with hemorrhagic fever with renal syndrome. a total of 106 samples from people who live in a marginal area in buenos aires city and 29 rattus norvegicus captured in the surroundings of their houses were tested for specific antibodies. thirty five samples from hypertensive patients undergoing a follow up health program, living in the same neighb ... | 2003 | 12876901 |
| expression of the nucleoprotein of the puumala virus from the recombinant semliki forest virus replicon: characterization and use as a potential diagnostic tool. | puumala virus (bunyaviridae family, hantavirus genus) causes a mild form of hemorrhagic fever with renal syndrome (hfrs) called nephropathia epidemica in northern and central europe. serological tests are used for diagnosis, but antigen production is difficult because the virus grows poorly in tissue culture. we expressed the n protein (nucleoprotein) of puumala virus via the semliki forest virus (sfv) replicon in mammalian cells and compared its antigenic properties with those of the native ant ... | 2003 | 12853401 |
| hantavirus-specific cd8(+)-t-cell responses in newborn mice persistently infected with hantaan virus. | the relationship between virus-specific cd8(+)-t-cell responses and viral persistence was studied in mice by using hantaan virus (htnv). we first established a simple method for measuring levels of virus-specific cd8(+) t cells by flow cytometry. next, to produce a mouse model of persistent htnv infection, newborn mice were inoculated subcutaneously within 24 h of birth with 1 or 0.1 50% newborn mouse lethal dose of htnv. all mice that escaped lethal infection were persistently infected with htn ... | 2003 | 12857910 |
| genetic characterisation of a hantavirus isolated from a laboratory-acquired infection. | seoul (seo) viruses belong to the hantavirus genus (family bunyaviridae), cause the moderate form of haemorrhagic fever with renal syndrome, and have been associated with laboratory-acquired infections in many countries. to investigate the pedigree of an isolate, designated ir461, which was obtained from a laboratory-acquired infection in a uk research institute, we determined the nucleotide sequences of the small (s) and medium (m) genome segments. in addition, we determined the sequences of th ... | 2003 | 12858415 |
| hantavirus infections in spain: analysis of sera from the general population and from patients with pneumonia, renal disease and hepatitis. | hantaviruses are rodent borne viruses in the family bunyaviridae that cause significant morbidity in large areas of europe. there are only a few reports available on hantavirus infections from spain. although the results of these earlier studies indicated the presence of hantavirus infections, no confirmative or serotype-specific analyses have been performed. | 2003 | 12878094 |
| adaptation of puumala hantavirus to cell culture is associated with point mutations in the coding region of the l segment and in the noncoding regions of the s segment. | we previously developed a model for studies on hantavirus host adaptation and initiated genetic analysis of puumala virus variants passaged in colonized bank voles and in cultured vero e6 cells. with the data presented in this paper, the sequence comparison of the wild-type and vero e6-adapted variants of puumala virus, strain kazan, has been completed. the only amino acid substitution that distinguished the two virus variants was found in the l protein, ser versus phe at position 2053. another ... | 2003 | 12885898 |
| [hantavirus infections in ile-de-france]. | hantavirus infections, puumala serotype, is a well-known disease in the northeast of france, but not in the paris area, despite regularly diagnosed cases. a retrospective study was performed from january 1999 to december 2000 to assess the clinical and epidemiological characteristics of this disease in the "région ile-de-france". fourteen cases were diagnosed. all required hospitalisation. patients presented usually with high fever, pain, renal failure, moderate thrombocytopenia and, sometimes, ... | 2003 | 12891831 |
| hantavirus prevalence in the ix region of chile. | an epidemiologic and seroprevalence survey was conducted (n=830) to assess the proportion of persons exposed to hantavirus in ix region chile, which accounts for 25% of reported cases of hantavirus cardiopulmonary syndrome. this region has three geographic areas with different disease incidences and a high proportion of aboriginals. serum samples were tested for immunoglobulin (ig) g antibodies by enzyme-linked immunosorbent assay against sin nombre virus n antigen by strip immunoblot assay agai ... | 2003 | 12890323 |
| [antigenic and genetic properties of seoul virus strains--a causative agent of hemorrhagic fever with renal syndrome]. | data on the circulation of a new genetic variant of seol virus, which was designated as "vladivostok" (the name of the place where it was isolated)--vdv, were obtained, by using the antigenic and genetic analysis of hantavirus strains isolated from patients with hemorrhagic fever with renal syndrome (hfrs) and from brown rats, and described in the present paper. a complete identity of nucleotide sequences of the m-segment fragment of isolates obtained from hfrs patients and of those obtained fro ... | 2003 | 12894473 |
| yellow pigmy rice rat (oligoryzomys flavescens) and hantavirus pulmonary syndrome in uruguay. | during 5,230 trapping nights, 672 small mammals were trapped in the areas where most hantavirus pulmonary syndrome (hps) cases occur in uruguay. yellow pygmy rice rats (oligoryzomys flavescens) were the only rodents that showed evidence of antibodies to hantavirus, with a seroprevalence of 2.6%. the rodents were trapped in all the explored environments, and most of the seropositive rodents were found in habitats frequented by humans. nucleotide sequences were obtained from four hps case-patients ... | 2003 | 12890326 |
| vaccines against hantaviruses. | rodent-borne hantaviruses are etiologic agents for hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome in the old world and new world, respectively. these often severe diseases are relatively uncommon in most parts of the world and are sufficiently genetically variable that widely cross-protective vaccines will probably need to be polyvalent. the current status of hantavirus vaccines shall be reviewed and both conventional and speculative new vaccine technologies that m ... | 2002 | 12901576 |
| [seroepidemiological studies of zoonotic infections in hunters in southeastern austria--prevalences, risk factors, and preventive methods]. | the aim of this study was to investigate the seroprevalences to zoonotic pathogens in hunters, to propose preventive measures and to obtain more information about the occurrence of zoonotic pathogens in local wild animal populations. from 146 male and 3 female hunters originating from the south-eastern austrian federal states of styria and burgenland blood samples were taken and anamnestic data were obtained using a questionnaire. the serological investigations included the following viral, bact ... | 2003 | 12894685 |
| serological analysis of hemorrhagic fever with renal syndrome (hfrs) patients in far eastern russia and identification of the causative hantavirus genotype. | hemorrhagic fever with renal syndrome (hfrs) is endemic in east asia and europe. the disease is caused by several viruses belonging to the genus hantavirus, including the hantaan virus (htnv), seoul virus (seov), dobrava belgrade virus (dobv), and puumala virus (puuv). recently, htnv-related viruses, amur (amr) and far east (fe) genotypes were identified as causative agents of hfrs in far eastern russia. to investigate the epidemiology of hfrs and virus transmission, we collected sera from 17 ac ... | 2003 | 12898330 |
| hantaviruses in são paulo state, brazil. | | 2003 | 12899143 |
| acute respiratory failure with thrombocytopenia in a 47-year-old woman after hiking in the sierras. | | 2003 | 12907570 |
| rapid and simple method for screening wild rodents for antibodies to sin nombre hantavirus. | sin nombre hantavirus (snv) is the primary etiologic agent of hantavirus cardiopulmonary syndrome (hcps) in the united states and canada. hantavirus cardiopulmonary syndrome is a zoonotic disease. the most common reservoir is the deer mouse (peromyscus maniculatus), although numerous other species of wild rodent can carry the viruses that cause hcps throughout the americas. infected rodents show no signs of clinical disease but they develop persistent infection. sin nombre virus can be contracte ... | 2003 | 12910753 |
| hantavirus antibody occurrence in bank voles (clethrionomys glareolus) during a vole population cycle. | puumala virus, genus hantavirus, is the etiologic agent of nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. the bank vole (clethrionomys glareolus) is the natural reservoir species of this hantavirus. we initiated sampling of bank voles at sites of recently identified human nephropathia epidemica cases and paired control sites in the fall of 1995 in coastal areas of northern sweden. sites were trapped annually in spring and fall until 1999. prevalence of antibody to ... | 2003 | 12910756 |
| analyses of gene flow among populations of deer mice (peromyscus maniculatus) at sites near hantavirus pulmonary syndrome case-patient residences. | gene flow and potential for sin nombre virus (snv) trafficking of the deer mouse (peromyscus maniculatus) was studied in delta and mesa counties of western colorado (usa). the study areas included grand mesa and surrounding grazing and agricultural areas. this area has several natural potential barriers to rodent gene flow, including rivers, cliffs, and mountains. ten study sites were utilized in a spatially nested design ranging from 0.65-81 km apart; four of these sites were at or near human h ... | 2003 | 12910755 |
| a cophylogenetic perspective of rna-virus evolution. | the extent to which viruses and their hosts codiverge remains an open question, given that numerous cases of both "cospeciation" and horizontal switching have recently been documented. dna viruses that form persistent infections are thought to be the most likely candidates for phylogenetic congruence. phylogenetic reconciliation analysis was used to compare established phylogenies for four rna viruses and their hosts. the analysis employs a cophylogeny mapping technique, implemented in treemap v ... | 2004 | 12949128 |
| hemorrhagic fever with renal syndrome presenting with intrauterine fetal death. a case report. | hantavirus infection in pregnancy is rare. only 2 cases of hantavirus pulmonary syndrome have been reported in the english-language literature. we report a case of hemorrhagic fever with renal syndrome (hfrs) complicating pregnancy to alert clinicians to this rare possibility. | 2003 | 12971153 |
| synthesis of seoul virus rna and structural proteins in cultured cells. | seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs). the virion has a tripartite (s, m, and l) negative-stranded rna genome, which is characteristic of the family bunyaviridae. however, the molecular basis of virus replication is not well known. we established a northern blot hybridization (nb) procedure using digoxygenin-labeled rna probes, to quantitate the hantaviral plus- and minus-strand rnas separately. virus rna replication was analyzed in infected vero e6 ... | 2003 | 14505081 |
| active and passive vaccination against hantavirus pulmonary syndrome with andes virus m genome segment-based dna vaccine. | hantavirus pulmonary syndrome (hps) is a rapidly progressing human disease with one of the highest case fatality rates (30 to 50%) of any acute viral disease known. there are no vaccines, effective antiviral drugs, or immunologics to prevent or treat hps. in an attempt to develop hps medical countermeasures, we constructed an expression plasmid, pwrg/and-m, that contains the full-length m genome segment of andes virus (andv), a south american hantavirus. transfection experiments in cell culture ... | 2003 | 12941899 |
| puumala virus pulmonary syndrome in a romanian immigrant. | hantaviruses belong to the bunyaviridae family, which is comprised of bunyavirus, phlebovirus, nairovirus, and hantavirus. euroasiatic hantaviruses belong to two distinct subfamilies: murinae (comprising hantaan, dobrava, and seoul viruses), which are responsible for hemorrhagic fever with renal syndrome (hfrs), and arvicolinae (comprising puumala virus), responsible for nephropathia epidemica (ne) and hfrs. on the contrary, the new world hantavirus belongs to the sigmodontinae subfamily and inc ... | 2002 | 12962589 |
| serological survey of hantavirus in jardinopolis county, brazil. | hantaviruses are the causative agents of severe human diseases such as cardiopulmonary syndrome (cps), described for the first time in 1993. approximately 200 cases of this emergent disease were reported in brazil until august 2002, with a 40% fatality rate. in may 2001, a hantavirus serologic survey was carried out among 15-70-year-old inhabitants of jardinopolis county, state of são paulo, brazil. blood samples of 818 inhabitants from urban and rural areas were collected by digitopuncture and ... | 2003 | 12966548 |
| tyrosine residues direct the ubiquitination and degradation of the ny-1 hantavirus g1 cytoplasmic tail. | the hantavirus g1 protein contains a long c-terminal cytoplasmic tail of 142 residues. hantavirus pulmonary syndrome-associated hantaviruses contain conserved tyrosine residues near the c terminus of g1 which form an immunoreceptor tyrosine activation motif (itam) and interact with src and syk family kinases. during studies of the g1 itam we observed that fusion proteins containing the g1 cytoplasmic tail were poorly expressed. expression of g1 cytoplasmic tail constructs were dramatically enhan ... | 2003 | 14512526 |
| hantavirus infection in humans and rodents, northwestern argentina. | we initiated a study to elucidate the ecology and epidemiology of hantavirus infections in northern argentina. the northwestern hantavirus pulmonary syndrome (hps)-endemic area of argentina comprises salta and jujuy provinces. between 1997 and 2000, 30 hps cases were diagnosed in jujuy province (population 512,329). most patients had a mild clinical course, and the death rate (13.3%) was low. we performed a serologic and epidemiologic survey in residents of the area, in conjunction with a serolo ... | 2003 | 14519242 |
| mapping of the regions involved in homotypic interactions of tula hantavirus n protein. | hantavirus nucleocapsid (n) protein has been suggested to form homodimers and homotrimers that are further integrated into the nucleocapsid filaments around the viral rna. here we report detailed mapping of the regions involved in the homotypic n protein interactions in tula hantavirus (tulv). peptide scan screening was used to define the interaction regions, and the mammalian two-hybrid assay was used for the functional analysis of n protein mutants. to study linear regions responsible for n pr ... | 2003 | 14512541 |
| hantavirus infections in europe. | hantaviruses are enveloped rna viruses each carried by a specific rodent species. three hantaviruses, puumala, dobrava, and saaremaa viruses, are known to cause haemorrhagic fever with renal syndrome. in europe. puumala causes a generally mild disease, nephropathia epidemica, which presents most commonly with fever, headache, gastrointestinal symptoms, impaired renal function, and blurred vision, whereas dobrava infections often also have haemorrhagic complications. there are few available data ... | 2003 | 14522264 |
| quality control measures for the serological diagnosis of hantavirus infections. | with society's rapidly increasing mobility, patients infected with severe viral infections can become seriously ill at any place in europe and elsewhere. improving the diagnostics of these infections is the most important step in detecting the pathogens and dealing with them, and for this purpose, quality control measures are essential tools. | 2003 | 14522063 |
| complete sequence of the genome of the human isolate of andes virus chi-7913: comparative sequence and protein structure analysis. | we report here the complete genomic sequence of the chilean human isolate of andes virus chi-7913. the s, m, and l genome segment sequences of this isolate are 1,802, 3,641 and 6,466 bases in length, with an overall gc content of 38.7%. these genome segments code for a nucleocapsid protein of 428 amino acids, a glycoprotein precursor protein of 1,138 amino acids and a rna-dependent rna polymerase of 2,152 amino acids. in addition, the genome also has other orfs coding for putative proteins of 34 ... | 2003 | 14513715 |
| detection of antibody for the serodiagnosis of hantavirus infection in different rodent species. | peroxidase-labeled staphylococcal protein a, streptococcal protein g, and antibodies directed against mus musculus (mouse), rattus norvegicus (rat), mesocretus auratus (hamster), and peromyscus leucopus were examined for their reactivity with immunoglobulin g (igg) from various rodent species. the purpose of this study was to identify the optimal secondary antibodies or reagents for specific serodiagnosis of hantavirus infection in various rodent species. using elisa, a total of 65 sera from 29 ... | 2003 | 14551813 |
| prevalence of antibodies against hantaviruses among the adult population of the czech republic. | at least three hantavirus genospecies are circulating among wild rodents in europe: puumala, dobrava and tula, the two first being of clinical significance for humans as possible causative agents of haemorrhagic fever with renal syndrome. as many as 710 adult individuals randomly selected from 12 regions of the czech republic were screened for the presence of antibodies against hantaviruses. commercial sets with hantaan and puumala antigens were used for this purpose. five subjects showed igg re ... | 2003 | 14514172 |
| occurrence of renal and pulmonary syndrome in a region of northeast germany where tula hantavirus circulates. | hantavirus species tula (tulv) is carried by european common voles (microtus spp.). its pathogenic potential for humans is unknown. in a rural region of northeast germany, a 43-year-old man became ill with fever, renal syndrome, and pneumonia. typing of late acute- and convalescent-phase sera by focus reduction neutralization assay revealed the presence of neutralizing antibodies against tulv. moreover, we detected tulv genetic material in microtus arvalis animals that were trapped at places onl ... | 2003 | 14532254 |
| urinary neopterin concentrations in patients with balkan endemic nephropathy (ben). | balkan endemic nephropathy (ben) is of great clinical importance in restricted areas of bulgaria, serbia, croatia, bosnia, herzegovina, and romania, since the etiology of ben is still unknown. | 2003 | 14531816 |
| the dynamics of two viral infections in a single host population with applications to hantavirus. | an si epidemic model for a host with two viral infections circulating within the population is developed, analyzed, and numerically simulated. the model is a system of four differential equations which includes a state for susceptible individuals, two states for individuals infected with a single virus, one which is vertically transmitted and the other which is horizontally transmitted, and a fourth state for individuals infected with both viruses. a general growth function with density-dependen ... | 2003 | 14583172 |
| [hantavirus infection as a rodent borne zoonosis]. | | 2003 | 14556503 |
| [hantavirus-induced acute renal failure. a case report]. | a non-ischemic acute renal failure may indicate a hantavirus nephropathy, so that appropriate serological examinations should be done. a 19-year-old man had acutely fallen ill with fever, rhinitis, and arthralgia. clinically, he presented with a polyuric renal failure as well as hypertension, proteinuria, erythro- and leucocyturia. serologically, igg- and igm-hantavirus antibodies were found against the central european variant of hantavirus dobrava. at renal biopsy, tubular epithelial swelling, ... | 2003 | 14605850 |
| association of the nucleocapsid protein of the seoul and hantaan hantaviruses with small ubiquitin-like modifier-1-related molecules. | we performed yeast two-hybrid screening of a human kidney cell cdna library to study the biological role of the hantavirus nucleocapsid protein (np). we found that seoul virus (seov) and hantaan virus (htnv) nps were associated with small ubiquitin-like modifier (sumo)-1-interacting proteins pias1, piasxbeta, hipk2, chd3, and ttrap, which interacted with the sumo-1 conjugating enzyme (ubc-9) and sumo-1 in the yeast two-hybrid assay. interactions between the hipk2, chd3, and ttrap proteins and se ... | 2003 | 14609633 |
| the complex ecology of hantavirus in paraguay. | following an outbreak of hantavirus pulmonary syndrome (hps) in the paraguayan chaco in 1995, calomys laucha was identified as the rodent host for the hantavirus associated with these cases. to explore the possibility of additional hantaviruses in paraguay, we collected 636 mammals from 10 of the 17 departments. plasma from 27 animals in alto paraguay and boquer6n in the chaco and neembucú and itapúa in the eastern region had antibody to andes virus antigens. of these 27, five individuals (among ... | 2003 | 14628942 |
| genetic similarity of puumala viruses found in finland and western siberia and of the mitochondrial dna of their rodent hosts suggests a common evolutionary origin. | a total of 678 small mammals representing eight species were trapped in western siberia in 1999-2000 and assayed for the presence of hantaviruses. eighteen animals, all clethrionomys species, were antigen positive by enzyme-linked immunosorbent assay (elisa). small and medium genome segments were recovered by rt-pcr from six samples from clethrionomys glareolus and three from clethrionomys rufocanus. sequence comparison and phylogenetic analysis revealed that these hantaviruses were puumala viru ... | 2003 | 14636686 |
| generation of a recombinant cytomegalovirus for expression of a hantavirus glycoprotein. | a cytomegalovirus (cmv) was isolated from its natural host, peromyscus maniculatus, and was designated peromyscus cmv (pcmv). a recombinant pcmv was constructed that contained sin nombre virus glycoprotein g1 (snv-g1) fused in frame to the enhanced green fluorescent protein (egfp) gene inserted into a site homologous to the human cmv ul33 (p33) gene. the recombinant cmv was used for expression and immunization of deer mice against snv-g1. the results of the study indicate that p. maniculatus cou ... | 2003 | 14581557 |
| hantavirus infections in europe. | | 2003 | 14652198 |
| the changing face of infectious disease. new threats, new science. | | 2003 | 14593993 |
| prevalence of infection with hantavirus in rodent populations of central argentina. | we studied hantavirus seroprevalence and virus variability in rodent populations in diego gaynor, northwest of buenos aires province, argentina. rodent samplings were conducted in railroads and cropfield borders in march and july 1999, september and december 2000, and march 2001. antibody detection was performed by an enzyme link immunosorbent assay (elisa), using the recombinant nucleoprotein of andes (and) virus as antigen. tissue samples were taken from positive antibody individuals in order ... | 2003 | 14595446 |
| tropical dermatology: viral tropical diseases. | viruses are important pathogens in tropical areas; most of them, especially the tropical hemorrhagic fevers, produce mucocutaneous manifestations. more than any other kind of pathogen, viruses have the possibility for being widespread, since they have a greater probability of mutation than do bacteria, can cross species barriers easily, and infect both human beings and animals in habitats with a great biodiversity. tropical habitats also have been subject to major ecologic changes in the last fe ... | 2003 | 14639375 |
| inhibition of dugbe nairovirus replication by human mxa protein. | sensitivity to the interferon-induced protein, mxa, has previously been demonstrated for viruses belonging to the orthobunyavirus, hantavirus and phlebovirus genera of the bunyaviridae family. we have extended these findings to a member of the fourth and remaining genus containing viruses that infect man and other animals, the nairovirus dugbe virus (dugv). indirect immunofluorescence experiments using va9 cells (vero cells permanently transfected with mxa cdna) revealed strongly reduced dugv an ... | 2004 | 14687945 |
| [hantavirus cardiopulmonary syndrome: utility of the picco(pulse contour cardiac output) system for monitoring]. | we report a 68 years old man, farmer from the metropolitan region, admitted with a hantavirus cardiopulmonary syndrome. the diagnosis was made using serologic test and was later confirmed by the public health institute. he evolved to an early multiple organ failure, requiring high concentrations of oxygen and invasive ventilatory assistance, vasopressor drugs and renal replacement therapy. swan ganz and picco were used simultaneously for hemodynamic monitoring. treatment consisted in global supp ... | 2003 | 14692308 |
| hantavirus infection in children. | this article focuses on recent developments in knowledge about hantavirus infections and hantavirus cardiopulmonary syndrome in children. we highlight clinical characterization, epidemiology, pathogenesis, diagnostic techniques, and current alternatives for treatment and prevention. | 2004 | 14758117 |
| prevalence of rabies virus and hantaan virus infections in commensal rodents and shrews trapped in bangkok. | five hundred rodents and shrews (rattus norvegicus: 458, rattus rattus: 28, rattus exulans: 5, mus musculus: 4 and suncus murine: 5) trapped from the fresh food markets around bangkok area were investigated for rabies virus and hantaan virus infections. no rabies viral antigens in the animals' brains were detected by direct immunofluorescence. on the other hand, antibodies to hantaan virus were demonstrated in the sera of 7 (1.53%) r. norvegicus caught in various markets using a particle aggluti ... | 2003 | 14696782 |
| bovine aortic endothelial cells are susceptible to hantavirus infection; a new aspect in hantavirus ecology. | hantaviruses are enveloped rna viruses that belong to the family bunyaviridae. they are the causative agents of hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hantaviruses show a worldwide distribution with specific rodent species as natural hosts. it is known that rodents can transmit the virus via feces, urine, saliva, or bites to humans. additionally, antibodies against different hantaviruses were also found in domestic animals, for example, danes et al. ... | 2004 | 14972540 |
| human hantavirus infections, sweden. | the prevalent human hantavirus disease in sweden is nephropathia epidemica, which is caused by puumala virus and shed by infected bank voles (clethrionomys glareolus). to evaluate temporal and spatial patterns of this disease, we studied 2,468 reported cases from a highly disease-endemic region in northern sweden. we found that, in particular, middle-aged men living in rural dwellings near coastal areas were overrepresented. the case-patients were most often infected in late autumn, when engaged ... | 2003 | 14718081 |
| hantavirus pulmonary syndrome, southern chile. | we analyzed data from 25 consecutive patients with hantavirus pulmonary syndrome (hps) admitted to the puerto montt and osorno regional hospitals, southern chile, from 1997 to 2001, emphasizing epidemiologic, clinical, radiographic, treatment, and laboratory aspects. hemorrhage was frequent (64%), and 48% of patients showed alterations in renal function. ten patients died (40%). we identified three groups of patients, which included the following: 1) those with the least severe form who had prod ... | 2003 | 14718088 |
| role of specific cd8+ t cells in the severity of a fulminant zoonotic viral hemorrhagic fever, hantavirus pulmonary syndrome. | we report on the role of specific cd8(+) t cells in the pathogenesis of a highly lethal human viral disease, hantavirus pulmonary syndrome (hps). hps is a zoonotic disease caused by transmission of sin nombre virus (snv) from chronically infected deer mice. in humans, this fulminant infection is characterized by lung capillary leakage, respiratory failure, and cardiogenic shock. individuals with hla-b*3501 have an increased risk of developing severe hps, suggesting that cd8(+) t cell responses t ... | 2004 | 14978138 |
| first molecular identification of human dobrava virus infection in central europe. | viral rna was amplified by reverse transcription-pcr from a patient suffering from hemorrhagic fever with renal syndrome (hfrs) in germany. the virus strain could be assigned to the dobrava hantavirus (dobv). this is the first molecular identification of human infection by dobv in central europe and the first proof that a virus strain related to the dobv-aa lineage, carried by apodemus agrarius rodents, is able to cause hfrs. | 2004 | 15004109 |
| bibliography. current world literature. infectious diseases and immunization. | | 2004 | 14971394 |
| microarray technology for identification and distinction of hantaviruses. | dna microarrays combine high-precision technology with advanced molecular biology to achieve high-throughput screening of dna fragments. in this study, we investigated the potential of the cdna microarray technique to identify and discriminate pcr derived amplicons from genetically highly similar viruses. the wide range of sequence variation among hantaviruses makes them suitable as a model for this purpose. the hantaviruses, carried by rodents, cause several hundred thousand cases of severe hum ... | 2004 | 14981768 |
| outbreaks of hantavirus induced by seasonality. | using a model for rodent population dynamics, we study outbreaks of hantavirus infection induced by the alternation of seasons. neither season by itself satisfies the environmental requirements for propagation of the disease. this result can be explained in terms of the seasonal interruption of the relaxation process of the mouse population toward equilibrium, and may shed light on the reported connection between climate variations and outbreaks of the disease. | 2004 | 14995490 |
| [is permanent renal function damage possible after hemorrhagic fever with renal syndrome?]. | to investigate the possibility of permanent renal function impairment and other organ lesions following hemorrhagic fever with renal syndrome (hfrs). | 2003 | 15011462 |
| [puumala and dobrava viruses in the northeastern and central regions of bosnia]. | bosnia and herzegovina has been known as a highly endemic region for hantavirus infections for more than 50 years. previous studies have shown that at least two different hantaviruses, the murine dobrava (dob) and avricoline puumala (puu) viruses, each carried by a different rodent species, have been circulating in the area. however, there is little information on rodent population density fluctuations in bosnia over the past years as well as on the ratio of puumala to dobrava infection in human ... | 2003 | 15011464 |
| [serologic diagnosis of hemorrhagic fever with renal syndrome in bosnia and herzegovina in 2002]. | results of the serologic confirmation of hemorrhagic fever with renal syndrome (hfrs) in patients with clinical picture and overt symptoms of the disease from particular areas in bosnia and herzegovina during 2002 are presented. | 2003 | 15011465 |
| [what is new in the epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia?]. | the aim was to describe epidemiologic characteristics of hemorrhagic fever with renal syndrome in croatia. | 2003 | 15011468 |
| [immunopathogenesis of hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome]. | hantaviruses (htv) cause two serious human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps), posing a considerable public health problem worldwide. immunopathogenesis has been suggested to be involved in both hfrs and hps. a common feature of hantavirus diseases is an increased microvascular bed permeability, suggesting that vascular endothelium is a prime target of virus infection. although htv infect numerous cell lines and primary cells, it was co ... | 2003 | 15011469 |
| [ocular changes in hemorrhagic fever with renal syndrome]. | hemorrhagic fever with renal syndrome (hfrs) is a rodent-originating zoonotic disease caused by puumala and dobrava viruses, which belong to the genus hantavirus. the most prominent ocular change in hfrs is transient myopia, which lasts for up to one week and is due to a forward movement of the ocular anterior diaphragm and thickening of the lens. other ophthalmic manifestations of hfrs are eyelid edema, conjunctival injection with or without hemorrhages, chemosis, anterior uveitis, retinal edem ... | 2003 | 15011470 |
| [hemorrhagic fever with renal syndrome: imported or native disease in the zadar region]. | the aim of the study is to warn of the need of clinical hemorrhagic fever with renal syndrome (hfrs) recognition and determination, and of the risk of its spread from a new focus of this natural zoonosis near zadar (north dalmatia), i.e., in the northern part (lika) of the zadar county. | 2003 | 15011474 |
| segments of puumala hantavirus nucleocapsid protein inserted into chimeric polyomavirus-derived virus-like particles induce a strong immune response in mice. | insertion of a short-sized epitope at four different sites of yeast-expressed hamster polyomavirus major capsid protein vp1 has been found to result in the formation of chimeric virus-like particles. here, we demonstrate that the insertion of 45 or 120 amino acid-long segments from the n-terminus of puumala hantavirus nucleocapsid protein into sites 1 (amino acids 80-89) and 4 (amino acids 288-295) of vp1 allowed the highly efficient formation of virus-like particles. in contrast, expression lev ... | 2004 | 15018662 |
| yeast-expressed hantavirus dobrava nucleocapsid protein induces a strong, long-lasting, and highly cross-reactive immune response in mice. | in europe, dobrava virus (dobv) carried by the yellow-necked field mouse apodemus flavicollis is one of the hantaviruses that can cause severe hemorrhagic fever with renal syndrome in humans. for several hantaviruses, the nucleocapsid (n) protein has proven to be very immunogenic in humans and rodents and even can protect rodents against a virus challenge. to investigate the immunogenicity of dobv n protein, balb/c and c57bl/6 mice were immunized three times with a dobv recombinant n (rn) protei ... | 2004 | 15018668 |
| [clinical and epidemiologic characteristics of hemorrhagic fever with renal syndrome in patients treated at the dr. fran mihaljević clinic for infectious diseases in zagreb]. | the aim of the study was to examine and analyze the main epidemiologic and clinical data of 94 patients with hemorrhagic fever with renal syndrome (hfrs) hospitalized at the university hospital for infectious diseases in zagreb during the hfrs outbreak in croatia in 2002. | 2003 | 15011459 |
| [an epidemic of hemorrhagic fever with renal syndrome at the therapeutic community for treatment of psychoactive drug dependence in ivanovac]. | the outbreak of hemorrhagic fever with renal syndrome (hfrs) at the ivanovac drug therapeutic community near nova gradiska was analyzed. the major epidemiologic, clinical and serologic findings are presented. | 2003 | 15011461 |
| [grading the severity of disease in patients with puumala or dobrava virus infections from 1995 to 2000 in croatia]. | the aim of our study was to evaluate the proposed croatian scale for grading the disease severity in patients with hemorrhagic fever with renal syndrome (hfrs). the aim was also to determine whether the infection with dobrava virus (dobv) in croatia was associated with a more severe illness than the infection with puumala virus (puuv). | 2003 | 15011460 |
| peridomestic small mammals associated with confirmed cases of human hantavirus disease in southcentral chile. | cases of human hantavirus disease have been reported in chile since 1995, most of them in people living in rural and periurban areas. we conducted a peridomestic study of small mammals to evaluate the relationships between the presence of rodents with antibodies to andes virus confirmed human cases of hantavirus pulmonary syndrome in southcentral chile. the results of 20 sampled sites, which involved the capture of 272 mice over an 18-month period, showed the occurrence of 10 small mammal specie ... | 2004 | 15031522 |
| genetic and antigenic characterization of the amur virus associated with hemorrhagic fever with renal syndrome. | the genetic and antigenic characteristics of the amur (amr) and far east (fe) virus lineages, which are both within the genus hantavirus, were studied. representative viruses, h5 and b78 for amr and bao 14 for fe, were used. the entire small (s) and medium (m) segments, except for the 3'- and 5'-ends, were sequenced. the deduced amino acid sequences of amr had 96.7 and 92.0-92.2% identities with the hantaan (htn) virus in the s and m segments, respectively. the amino acid sequences of fe had 99. ... | 2004 | 15041180 |
| prevalence of antibodies to hantavirus among family and health care worker contacts of persons with hantavirus cardiopulmonary syndrome: lack of evidence for nosocomial transmission of andes virus to health care workers in chile. | nosocomial transmission of andes virus has been documented in argentina, but has not yet been proven in chile. we studied 215 contacts (106 family member contacts and 109 health care worker contacts) of 20 index cases of hantavirus cardiopulmonary syndrome (hcps) in chile. the seroprevalence of igg antibodies against andes virus was 1.9% (95% confidence interval [ci] = 0.34-6.3%) among the family members and 0.0% (95% ci = 0-3.2%) among the health care workers. our data suggest that there is no ... | 2004 | 15031521 |
| bovine aortic endothelial cells are susceptible to hantaan virus infection. | hantavirus serotype hantaan (htn) is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs, lethality up to 10%). the natural host of htn is apodemus agrarius. recent studies have shown that domestic animals like cattle are sporadically seropositive for hantaviruses. in the present study, the susceptibility of bovine aortic endothelial cells (baec) expressing alpha(v)beta(3)-integrin to a htn infection was investigated. viral nucleocapsid protein and genomic rna segments wer ... | 2004 | 15033559 |
| spatial and temporal analysis of the distribution of hantavirus pulmonary syndrome in buenos aires province, and its relation to rodent distribution, agricultural and demographic variables. | we studied the spatial and temporal distribution of hantavirus pulmonary syndrome (hps) cases from 1998 to 2001 in the buenos aires province, argentina. hps is a severe viral disease whose natural reservoir are rodents of the subfamily sigmodontinae (muridae) and which occurs in many countries of south and north america. we considered two spatial arrangements: cells of 18.5 x 18.5 km(2); and departments, the political subdivisions of the province, as spatial units. we tested the departure from a ... | 2004 | 15078270 |
| [new epidemiologic and molecular evidence of person to person transmission of hantavirus andes sout]. | after the hantavirus pulmonary syndrome (hps) outbreak occurred in southern argentina (patagonia) in 1996, a new mechanism of hantavirus spread was described for andes virus (and): person-to-person transmission. since then, this mode of transmission has been limited to the endemic area of and sout lineage. we report here an hps case, patient a, with residence in buenos aires province, whose unique epidemiological risk factor was to have shared a trip to southern argentina with an already ill per ... | 2004 | 15034956 |
| immune responses to puumala virus infection and the pathogenesis of nephropathia epidemica. | puumala virus, causative agent of a mild form of hemorrhagic fever with renal syndrome, also known as nephropathia epidemica, induces long-lasting humoral and cellular immunity in patients. the virus itself is not cytopathic, and the immune responses to the virus may be involved in teh pathogenesis of the disease. | 2004 | 15049335 |
| tula hantavirus l protein is a 250 kda perinuclear membrane-associated protein. | the complete open reading frame of tula hantavirus (tulv) l rna was cloned in three parts. the middle third (nt 2191-4344) could be expressed in e. coli and was used to immunize rabbits. the resultant antiserum was then used to immunoblot concentrated tulv and infected vero e6 cells. the l protein of a hantavirus was detected, for the first time, in infected cells and was found to be expressed as a single protein with an apparent molecular mass of 250 kda in both virions and infected cells. usin ... | 2004 | 15105534 |
| comparison of virulence of various hantaviruses related to hemorrhagic fever with renal syndrome in newborn mouse model. | the virulence of hantaviruses that are antigenically related but have different genetic characteristics from the prototype of hantavirus, hantaan (htn) virus, was examined in newborn mice. the h5 and b78 strains of the amur (amr) genotype, the bao14 strain of the far east (fe) genotype, and the 76-118 strain of htn virus were inoculated subcutaneously (1focus-forming unit; ffu) into newborn mice. all of the amr and fe genotype viruses inoculated mice were died by 16 days post-infection (dpi) and ... | 2004 | 15070039 |
| modes of hantavirus transmission in a population of clethrionomys rufocanus bedfordiae inferred from mitochondrial and microsatellite dna analyses. | to elucidate the mode of transmission of puumala-related hantavirus in a population of gray red-backed voles, clethrionomys rufocanus bedfordiae, in hokkaido, japan, we analyzed the kin structure and dispersal patterns of individual voles using microsatellite and mitochondrial dna markers. siblings or dam/offsprings was identified within the population based on the relatedness calculation with the microsatellite data. the pairwise relatedness values obtained could reveal kinship among all vole i ... | 2004 | 15098108 |
| prevalence of hantavirus antibody in patients with chronic renal disease in egypt. | in egypt, the etiology of chronic renal failure (crf) is not well defined. a hospital-based case-control study was initiated in february 1998, to determine whether hantavirus infection is involved in chronic renal disease (crd) in egypt. the study enrolled 350 study patients with a history of crf and 695 matched controls with crd due to renal calculus or renal cancer, but with normal renal functions. sera from cases and controls were tested for anti-hantavirus igg using elisa with a cell-lysate ... | 2004 | 15099987 |