| the hantaviral load in tissues of naturally infected rodents. | hantaviruses cause a lifelong and asymptomatic infection in naturally infected hosts as well as in experimentally infected rodents. understanding the ecology and pathogenesis of hantaviruses requires an interdisciplinary research approach, which links laboratory experiments with results gained from field studies. although several studies report hantavirus persistence and tissue infection patterns for experimentally infected rodents, field data is very limited. for this reason, the aim of our stu ... | 2009 | 19397875 |
| adenovirus vectors expressing hantavirus proteins protect hamsters against lethal challenge with andes virus. | hantaviruses infect humans following aerosolization from rodent feces and urine, producing hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. due to the high rates of mortality and lack of therapies, vaccines are urgently needed. nonreplicating adenovirus (ad) vectors that express andes hantavirus (andv) nucleocapsid protein (adn) or glycoproteins (adg(n) and adg(c)) were constructed. ad vectors were tested for their ability to protect syrian hamsters from a lethal andv inf ... | 2009 | 19403663 |
| replication reduction neutralization test, a quantitative rt-pcr-based technique for the detection of neutralizing hantavirus antibodies. | hantaviruses, which are mainly rodent-borne viruses, cause hemorrhagic fever with renal syndrome in the old world, and hantavirus pulmonary syndrome in the new world. a neutralization test based on quantitative rt-pcr, the replication reduction neutralization test (rrnt), was developed for efficient detection of hantavirus-neutralizing antibodies. the effectiveness of the rrnt was evaluated by examining several hantaviruses and hantavirus-specific convalescent human serum samples. all convalesce ... | 2009 | 19406158 |
| first molecular evidence of tula hantavirus in microtus voles in slovenia. | different microtus species, present in a worldwide range habitat populating north america, europe, asia, and few other species have been recognized previously as a hantavirus reservoir. tula hantavirus was first reported in microtus arvalis and microtus rossiaemeridionalis from central russia and later discovered in several european countries. using molecular techniques we have demonstrated the presence of tula hantavirus in three different microtus species in slovenia. phylogenetic analyses of ... | 2009 | 19410611 |
| experimental evidence for reduced rodent diversity causing increased hantavirus prevalence. | emerging and re-emerging infectious diseases have become a major global environmental problem with important public health, economic, and political consequences. the etiologic agents of most emerging infectious diseases are zoonotic, and anthropogenic environmental changes that affect wildlife communities are increasingly implicated in disease emergence and spread. although increased disease incidence has been correlated with biodiversity loss for several zoonoses, experimental tests in these sy ... | 2009 | 19421313 |
| is hantavirus important to practitioners? | | 2009 | 19434032 |
| hantavirus pulmonary syndrome: a concise clinical review. | in 1978, hantaviruses were first described as the etiological agent of hemorrhagic fever with renal syndrome (hfrs) in korea. since then, numerous related, enveloped, negative-stranded rna viruses have been identified, forming the genus hantavirus within the family bunyaviridae. these pathogens are distributed worldwide and thus can be classified, on the basis of phylogenetic origins, into old world viruses or new world viruses (ie north, central, and south america). similarly, these viruses cau ... | 2009 | 19434035 |
| phylogenetic characterization of hantaviruses from wild rodents and hantavirus pulmonary syndrome cases in the state of parana (southern brazil). | over 1,100 cases of hantavirus pulmonary syndrome (hps) have occurred in brazil since 1993, but little is known about brazilian hantaviruses, and many of their rodent hosts remain unknown. the araucaria hantavirus (arauv) was described recently from hps patients from paraná, in southern brazil, but its host could not be identified. in this study, rodents were captured from regions with high hps prevalence to address this issue. arauv rna was detected in three distantly related rodent species: ol ... | 2009 | 19439554 |
| new genetic lineage of tula hantavirus in microtus arvalis obscurus in eastern kazakhstan. | genomic sequences of tula (tulv) hantavirus were recovered from tissue samples of european common voles microtus arvalis (subspecies obscurus) captured in kazakhstan, central asia. phylogenetic analysis of the s genomic segment of kazakh tulv strains showed that they form distinct, well supported genetic lineage and share a more ancient common ancestor with two russian lineages of tulv. the deduced sequence of the nucleocapsid (n) protein of kazakh tulv strains carried specific amino acid signat ... | 2008 | 19440462 |
| an outbreak caused by hantavirus in the black sea region of turkey, january-may 2009. | we present a preliminary report of 12 laboratory-confirmed cases of haemorrhagic fever with renal syndrome (hfrs) in turkey, diagnosed between january and may 2009 according to the clinical symptoms and serological confirmation. studies are still ongoing to better understand the dynamics of the reservoir population as well as the epidemiological characteristics and risk factors among humans. | 2009 | 19460288 |
| gold nanoparticle enhanced immuno-pcr for ultrasensitive detection of hantaan virus nucleocapsid protein. | a functionalized gold nanoparticle (gnp) enhanced ultrasensitive immuno-pcr assay (gnp-ipcr on elisa plate), which was modified from the recent developed bio-barcode assay (bca) technique, was developed to detect hantaan virus nucleocapsid protein (hnp). during the assay, the target antigen hnp was captured by a polyclonal antibody coated on elisa microplate wells, followed by adding gnp dually modified with oligonucleotides and a hnp specific monoclonal antibody l13 (mab l13) to form a sandwich ... | 2009 | 19467237 |
| serological microarray for detection of hsv-1, hsv-2, vzv, and cmv antibodies. | the seroprevalence of human herpesviruses is high and reactivations occur frequently. a microarray was designed and tested for the detection of igg and igm antibodies for puumala hantavirus (puuv) and igg antibodies against four herpesviruses. initially, a microarray platform was set up using an unrelated in-house antigen, puuv recombinant nucleocapsid protein, to optimize the protocol for the detection of antibodies. detection of the four herpesviruses was set up in a microarray using the recom ... | 2009 | 19477202 |
| genetic diversity and geographic distribution of hantaviruses in russia. | haemorrhagic fever with renal syndrome (hfrs) is the most prevalent zoonotic disease in russia. it is caused by several hantavirus species hosted by small rodents. we describe spatial and temporal patterns of hfrs incidence in the russian federation, and the geographic distribution of prevalent hantavirus species: puumala (puuv) and dobrava (dobv). partial sequencing of nucleocapsid and glycoprotein genes of 117 puuv strains and 78 dobv strains revealed several distinct genetic subgroups. the rn ... | 2009 | 19486318 |
| testing mechanisms of the dilution effect: deer mice encounter rates, sin nombre virus prevalence and species diversity. | species diversity has been shown to decrease prevalence of disease in a variety of host-pathogen systems, in a phenomenon termed the dilution effect. several mechanisms have been proposed by which diversity may decrease prevalence, though few have been tested in natural host-pathogen systems. we investigated the mechanisms by which diversity influenced the prevalence of sin nombre virus (snv), a directly transmitted virus in deer mice (peromyscus maniculatus). we monitored both intra and intersp ... | 2009 | 19495881 |
| [the first french hemorrhagic fever with renal syndrome in pregnant woman]. | the seroprevalence of hantavirus in france is very low. the hantavirus is the causative agent of hemorrhagic fever with renal syndrome (hfrs). in 2005, we report the first french hfrs case in a pregnant woman presenting with influenza like symptoms in an endemic area associated with suggestive biologic abnormalities. she was delivered by emergency cesarean section. her child had a neonatal respiratory distress, thrombocytopenia and elevated liver enzymes levels, which suggest a transplacental ha ... | 2009 | 19497685 |
| phage display selection of cyclic peptides that inhibit andes virus infection. | specific therapy is not available for hantavirus cardiopulmonary syndrome caused by andes virus (andv). peptides capable of blocking andv infection in vitro were identified using antibodies against andv surface glycoproteins gn and gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified andv particles. phage was examined for andv infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. three peptides showed leve ... | 2009 | 19515773 |
| theory of possible effects of the allee phenomenon on the population of an epidemic reservoir. | we investigate possible effects of high-order nonlinearities on the shapes of infection refugia of the reservoir of an infectious disease. we replace fisher-type equations that have been recently used to describe, among others, the hantavirus spread in mouse populations by generalizations capable of describing allee effects that are a consequence of the high-order nonlinearities. after analyzing the equations to calculate steady-state solutions, we study the stability of those solutions and comp ... | 2009 | 19518251 |
| knowledge and practices about hantavirus pulmonary syndrome in a cluster of japanese communities in argentina. | to provide information on hantavirus pulmonary syndrome (hps) knowledge and practices among a cluster of the rural japanese communities near la plata in central argentina, an area with confirmed hps cases. particular emphasis was placed on testing the hypothesis that there would be differences between household use of hps prevention measures based on the head-of-household's knowledge of hps. | 2009 | 19531307 |
| [climate changes and emerging diseases. what new infectious diseases and health problem can be expected?]. | increasing temperatures, but also other climatic factors, will have an impact on human health. apart from the direct consequences of extreme weather conditions (e.g., heat-related fatalities), indirect health consequences in the long-term are also of great importance. in addition to a likely increase in allergic diseases and additional complications in the course of cardiovascular and respiratory diseases, infectious diseases are of particular interest. in germany, endemic pathogens, such as han ... | 2009 | 19536444 |
| genotyping of hantaviruses occurring in linyi, china, by nested rt-pcr combined with single-strand conformation polymorphism analysis. | the hemorrhagic fever with renal syndrome (hfrs) incidence rate still holds in high level in some areas of the world, but a reliable and simple typing method that can be used in clinical diagnosis and epidemiologic surveys is not available. in this study, 48 serum samples were collected from patients with hfrs in linyi area, china, that is seriously affected by this disease. the collected samples were analyzed by nested rt-pcr combined with single-strand conformation polymorphism (sscp) for geno ... | 2009 | 19537913 |
| [molecular characterization and sequence comparison of the m and s segments of hantavirus zj5 strain]. | in order to understand the molecular characters of hantavirus zj5 strain, its complete m and s genome were sequenced and compared with that of other hantavirus strains. | 2008 | 19544637 |
| [identification and analysis of an seo strain of hemorrhagic fever with renal syndrome hantavirus from guangxi]. | to identify hantavirus from lung specimens of rats captured in guangxi. | 2008 | 19544640 |
| young man with kidney failure and hemorrhagic interstitial nephritis. | | 2009 | 19556044 |
| hantavirus infection and habitat associations among rodent populations in agroecosystems of panama: implications for human disease risk. | hantavirus cardiopulmonary syndrome (hcps), which is caused by infection with choclo virus, is uncommon in panama, yet seropositivity among rural residents is as high as 60%. to clarify the environmental risk factors favoring rodent-to-human transmission, we tested serum from 3,067 rodents captured over a five-year period for antibodies against recombinant n protein of hantavirus by enzyme immunoassay and strip immunoblot. among 220 seropositive rodents, oligoryzomys fulvescens, the reservoir of ... | 2009 | 19556568 |
| environmental and ecological potential for enzootic cycles of puumala hantavirus in great britain. | puumala virus (puuv) is a zoonotic rodent-borne hantavirus in continental europe. its reservoir host, the bank vole (myodes glareolus), is ubiquitous in great britain (gb); however, there has been no reported incidence of virus in either animals or humans. in northwest europe, increases in bank vole numbers, stimulated by increases in production of beech/oak crops (mast), are associated with outbreaks of nephropathia epidemica (ne) in humans. these so-called 'mast years' are determined by sequen ... | 2010 | 19563697 |
| [cloning and expression of the nucleoprotein gene of puumala-like virus]. | in order to detect hokkaido virus (hokv), a recombinant baculovirus containing the nucleoprotein (np) gene of hokv was constructed, and then the np was expressed in insect cell. | 2009 | 19565881 |
| [study on the difference of genes and the type identification of hantavirus from lishui, zhejiang province]. | to isolate hantavirus from lishui county--one of the epidemic regions for hemorrhagic fever with renal syndrome (hfrs), in zhejiang province, and to identify the serotype and molecular/biological characteristics of a new htn subtype hantavirus (hv) strains, hopefully to provide evidence for hfrs prevention and therapy. | 2009 | 19565882 |
| hantaviral infections of rodents: possible scenarios. | | 2009 | 19568690 |
| the first established focus of hantavirus infection in poland, 2007. | the first hantavirus infection outbreak in poland (with different seroetiology) was identified between august-december 2007. thirteen cases were reported in southeast poland: 12 cases in the carpathians bordering with northeast slovakia, mainly in the forested areas of the bieszczady mountains, and one case approximately 100-120 kilometres north from the others, in the adjacent sub-carpathian region. four additional cases of past infection were identified retrospectively, based on the presence o ... | 2009 | 19572481 |
| evolutionary insights from a genetically divergent hantavirus harbored by the european common mole (talpa europaea). | the discovery of genetically distinct hantaviruses in shrews (order soricomorpha, family soricidae) from widely separated geographic regions challenges the hypothesis that rodents (order rodentia, family muridae and cricetidae) are the primordial reservoir hosts of hantaviruses and also predicts that other soricomorphs harbor hantaviruses. recently, novel hantavirus genomes have been detected in moles of the family talpidae, including the japanese shrew mole (urotrichus talpoides) and american s ... | 2009 | 19582155 |
| a single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus gn glycoprotein confers protective immunity against seoul virus in mice. | seoul virus (seov), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) and afflicts tens of thousands of people annually. in this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gn protein of seov (rcav-2-gn) in balb/c mice. sera from immunized mice contained neutralizing antibodies that could specifically recognize seov and neutralize its infectivity in vitro. ... | 2009 | 19583957 |
| hantavirus infection: a case report from india. | the clinical presentation of hantavirus infections in india is unclear. we report here a case of hantavirus infection in a 46 year old quarry worker presenting with fever, abdominal pain, jaundice, thrombocytopenia and renal dysfunction. seroconversion and rising anti-hantavirus igg titers were taken as evidence of hantavirus infection. clinicians should consider hantavirus infections in the differential diagnosis of acute febrile illness along with scrub typhus, leptospirosis and dengue. | 2009 | 19584514 |
| analysis of the immune response to hantaan virus nucleocapsid protein c-terminal-specific cd8(+) t cells in patients with hemorrhagic fever with renal syndrome. | hantaan virus (htnv), the prototype member of the hantavirus genus in the family bunyaviridae, causes hemorrhagic fever with renal syndrome (hfrs), which is characterized by capillary leakage, hemorrhage, and renal injury, and is an important public health problem in china. some kinds of immune cells, particularly cd8(+) t cells, are involved in the pathogenesis of hantavirus infection. the nucleocapsid protein (np) of the hantavirus is the most conserved structural protein and the most abundant ... | 2009 | 19594396 |
| temporal and geographic evidence for evolution of sin nombre virus using molecular analyses of viral rna from colorado, new mexico and montana. | all viruses in the family bunyaviridae possess a tripartite genome, consisting of a small, a medium, and a large rna segment. bunyaviruses therefore possess considerable evolutionary potential, attributable to both intramolecular changes and to genome segment reassortment. hantaviruses (family bunyaviridae, genus hantavirus) are known to cause human hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. the primary reservoir host of sin nombre virus is the deer mouse (peromyscus ... | 2009 | 19602267 |
| hantaviruses and climate change. | most hantaviruses are rodent-borne emerging viruses. they cause two significant human diseases, haemorrhagic fever with renal syndrome in asia and europe, and hantavirus cardiopulmonary syndrome in the americas. very recently, several novel hantaviruses with unknown pathogenic potential have been identified in africa and in a variety of insectivores (shrews and a mole). because there is very limited information available on the possible impact of climate change on all of these highly dangerous p ... | 2009 | 19604276 |
| hantavirus reservoir oligoryzomys longicaudatus spatial distribution sensitivity to climate change scenarios in argentine patagonia. | oligoryzomys longicaudatus (colilargo) is the rodent responsible for hantavirus pulmonary syndrome (hps) in argentine patagonia. in past decades (1967-1998), trends of precipitation reduction and surface air temperature increase have been observed in western patagonia. we explore how the potential distribution of the hantavirus reservoir would change under different climate change scenarios based on the observed trends. | 2009 | 19607707 |
| a habitat-based model for the spread of hantavirus between reservoir and spillover species. | new habitat-based models for spread of hantavirus are developed which account for interspecies interaction. existing habitat-based models do not consider interspecies pathogen transmission, a primary route for emergence of new infectious diseases and reservoirs in wildlife and man. the modeling of interspecies transmission has the potential to provide more accurate predictions of disease persistence and emergence dynamics. the new models are motivated by our recent work on hantavirus in rodent c ... | 2009 | 19616014 |
| increased host species diversity and decreased prevalence of sin nombre virus. | emerging outbreaks of zoonotic diseases are affecting humans at an alarming rate. until the ecological factors associated with zoonoses are better understood, disease emergence will continue. for lyme disease, disease suppression has been demonstrated by a dilution effect, whereby increasing species diversity decreases disease prevalence in host populations. to test the dilution effect in another disease, we examined 17 ecological variables associated with prevalence of the directly transmitted ... | 2009 | 19624913 |
| risk factors for human infection with puumala virus, southwestern germany. | puumala virus, which causes nephropathia epidemica (ne), is the most prevalent hantavirus in germany; bank voles serve as the main reservoir. during 2001-2007, most ne cases reported from germany occurred in the southwestern state of baden-württemberg. we investigated the influence of bank vole habitats (beech forest, seed plants), vole food supply (beechnut mast), climate factors (winter and spring temperatures), and human population density on spatial and temporal occurrence of ne cases in bad ... | 2009 | 19624917 |
| sensitivity of andes hantavirus to antiviral effect of human saliva. | | 2009 | 19624946 |
| andes virus infections in the rodent reservoir and in humans vary across contrasting landscapes in chile. | hantavirus cardiopulmonary syndrome (hcps) is an emerging infectious disease first reported in chile in 1995. andes hantavirus (andv) is responsible for the more than 500 cases of hcps reported in chile. previous work showed that andv is genetically differentiated in chile across contrasting landscapes. to determine whether the reservoir rodent (oligoryzomys longicaudatus) populations are also geographically segregated, we conducted range-wide spatial genetic analyses of o. longicaudatus in chil ... | 2010 | 19632357 |
| evaluation of an enzyme-linked immunosorbent assay based on araraquara virus recombinant nucleocapsid protein. | laboratory diagnosis of hantavirus cardiopulmonary syndrome (hcps) in brazil has been performed mostly by detection of igm antibodies to recombinant antigen purified from sin nombre virus and andes virus (andv). recently, a recombinant nucleocapsid (rn) protein of araraquara virus (arav), a brazilian hantavirus, was obtained in escherichia coli. to evaluate arav rn as antigen for antibody detection, serum samples from 30 patients from argentina seropositive for hantavirus were tested. all sample ... | 2009 | 19635882 |
| [survey on host animal and molecular epidemiology of hantavirus in chuxiong prefecture, yunnan province]. | to determine the hosts of hantavirus (hv) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (hfrs). | 2009 | 19642377 |
| production and characterization of monoclonal antibodies against the recombinant nucleoprotein of araucaria hantavirus. | hantaviruses are rodent-borne rna viruses that cause hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). from the first detection of infection in brazil in 1993 until 2009, 1161 cases of hps have been reported, with mortality rates of around approximately 40%. currently, due to the absence of a vaccine or specific antiviral therapy, the only way to reduce mortality by hantavirus infection is a fast and precise diagnosis that allows for supportive clinical health ... | 2009 | 19654026 |
| genetic characterization of a juquitiba-like viral lineage in oligoryzomys nigripes in rio de janeiro, brazil. | hantaviruses, family bunyaviridae, are rodent-borne rna viruses that have caused cases of hantavirus cardiopulmonary syndrome (hcps) in various regions of the americas. there are five hantaviral lineages associated with hcps in brazil: juquitiba virus (juqv), araraquara virus (arav), laguna negra-like virus (lnv), castelo dos sonhos virus (casv), and anajatuba virus (anajv). three additional hantaviruses have been described in rodents alone: rio mearim virus, jaborá virus, and a hantavirus linea ... | 2009 | 19660427 |
| degradation and aggresome formation of the gn tail of the apathogenic tula hantavirus. | the cytoplasmic tails of envelope glycoprotein gn of pathogenic hantaviruses but not of the apathogenic prospect hill virus (phv) were recently reported to be proteasomally degraded in simian cos7 cells. here, we show that the cytoplasmic tails of the glycoproteins of the apathogenic hantaviruses tula virus (tulv) and phv are also degraded through the ubiquitin-proteasome pathway, both in human hek-293 and in simian vero e6 cells. tulv gn tails formed aggresomes in cells with proteasomal inhibit ... | 2009 | 19675185 |
| [pulmonary and cardiovascular syndrome due to hantavirus: clinical aspects of an emerging disease in southeastern brazil]. | pulmonary and cardiovascular syndrome due to hantavirus is a disease caused by inhalation of aerosols from the excreta of wild rodents contaminated by viruses of the bunyaviridae family. we studied the clinical and laboratory manifestations of 70 cases that occurred in the region of ribeirão preto, sp, brazil, between 1998 and 2007. the frequency of symptoms was as follows: dyspnea (87%), fever (81%), coughing (44%), headache (34%), tachycardia (81%), low arterial blood pressure (56%), metabolic ... | 2009 | 19684976 |
| hantavirus infection: a review and global update. | hantaviruses have the potential to cause two different types of diseases in human: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). hfrs, initially described clinically at the turn of the 20th century, occurs endemically in the asian and european continents, while hps, recognized as a clinical entity since 1993, represents the prototype of emerging diseases occurring in the western hemisphere. approximately 150,000 to 200,000 cases of hfrs are hospitalized ea ... | 2008 | 19736383 |
| hantavirus species in india: a retrospective study. | hantaviruses cause hemorrhagic fever with renal syndrome in europe and asia. there are about 20 documented hantavirus species and newer species are being described worldwide, especially in non-rodent reservoirs, i.e shrews. focus reduction neutralization test is the classical serotyping technique for hantavirus. however, this study employs a previously established serotyping elisa, to retrospectively analyze known hantavirus igg reactive samples for infecting serotypes. the result suggests prese ... | 2009 | 19736405 |
| [serological characteristics of hantaviruses from clinical specimens analyzed in 1998-2008 in the department of virology, public health institute, ostrava]. | in 1998 - 2008, serum samples from 464 patients, predominantly residents of the moravia-silesian region, were examined in the department of virology, public health institute in ostrava, using enzyme-linked immunosorbent assay (elisa) for the detection of specific anti-hantavirus antibodies. seropositivity was ascertained in 41 persons (8.8%). more patients tested seropositive against hantaan virus (24, i.e. 58.5%) than against puumala virus (13, i.e. 31.7%). four persons were reactive against bo ... | 2009 | 19750821 |
| inhibition of tnf-alpha-induced activation of nf-kappab by hantavirus nucleocapsid proteins. | hantaviruses cause two human diseases thought to be immune-mediated: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). we recently reported that the nucleocapsid (n) protein of hfrs-causing hantaan virus (htnv) inhibits tumor necrosis factor-alpha (tnf-alpha) activation of nuclear factor-kappab (nf-kappab). here we measured the ability of other hantaviral n proteins to similarly interfere with the inflammatory process of tnf-alpha. we found that like htnv n, t ... | 2009 | 19751407 |
| identification of oligopeptides mimicking the receptor-binding domain of hantaan virus envelope glycoprotein from a phage-displayed peptide library. | attachment of enveloped viruses to cells is triggered by the receptor-binding domain (rbd) on envelope glycoproteins (gp) binding to receptors located on the cell surface. to date, recognized receptors and rbd of hantaan virus (htnv) have not been exactly defined. in this study, one monoclonal antibody (mab) 3g1 possessing high neutralizing activity, which is directed against htnv envelope glycoprotein g2, was used to determine the crucial motif of rbd. peptide ligands binding to mab 3g1 were se ... | 2009 | 19767836 |
| complete nucleotide sequence of four rna segments of fig mosaic virus. | the complete sequence of four viral rna segments of fig mosaic virus (fmv) was determined. each of the four rnas comprises a single open reading frame (orf) 7,093, 2,252, 1,490 and 1,472 nucleotides in size, respectively. these orfs encode the following proteins in the order: rna-dependent rna polymerase (p1 264 kda), a putative glycoprotein (p2 73 kda), a putative nucleocapsid protein (p3 35 kda) and a protein with unknown function (p4 40.5 kda). all rna segments possess untranslated regions co ... | 2009 | 19777155 |
| case-control study on puumala virus infection: smoking is a risk factor. | puumala hantavirus (puuv) is apparently transmitted to humans by inhalation of aerosolized secretions of carrier rodents (bank voles). the means of transmission and the associated risk factors are poorly defined. an epidemiological study during the peak of an epidemic season in finland was conducted based on 282 acute clinical puuv infections and 204 controls without puuv infection or immunity. the main risk factors adjusted by age, sex and living environment were cigarette smoking [odds ratio ( ... | 2010 | 19781114 |
| using satellite images of environmental changes to predict infectious disease outbreaks. | recent events clearly illustrate a continued vulnerability of large populations to infectious diseases, which is related to our changing human-constructed and natural environments. a single person with multidrug-resistant tuberculosis in 2007 provided a wake-up call to the united states and global public health infrastructure, as the health professionals and the public realized that today's ease of airline travel can potentially expose hundreds of persons to an untreatable disease associated wit ... | 2009 | 19788799 |
| hantavirus infection in the republic of georgia. | we describe a laboratory-confirmed case of hantavirus infection in the republic of georgia. limited information is available about hantavirus infections in the caucasus, although the infection has been reported throughout europe and russia. increasing awareness and active disease surveillance contribute to our improved understanding of the geographic range of this pathogen. | 2009 | 19788822 |
| hantavirus pulmonary syndrome in brasilia periphery: a diagnostic challenge. | hantavirus pulmonary syndrome (hps) is an emerging rodent-borne disease in the american continent, characterized by acute respiratory distress and a high case-fatality ratio. the present work describes a case of hps, with favorable outcome, whose initial features were mistaken for leptospirosis or other less severe acute infections. | 2009 | 19801809 |
| hantavirus transmission in sylvan and peridomestic environments. | we developed a compartmental model for hantavirus infection in deer mice (peromyscus maniculatus) with the goal of comparing relative importance of direct and indirect transmission in sylvan and peridomestic environments. a direct transmission occurs when the infection is mediated by the contact of an infected and an uninfected mouse, while an indirect transmission occurs when the infection is mediated by the contact of an uninfected mouse with, for instance, infected soil. based on population d ... | 2010 | 19821001 |
| hantavirus nephropathy as a pseudo-import pathology from ecuador. | we report a case of hantavirus infection (nephropathia epidemica) diagnosed in a belgian backpacker returning from a trekking expedition in ecuador, after likely heavy exposure to rodents. because of epidemiological inconsistency, molecular investigation was performed and revealed a puumala infection acquired during very limited exposure in belgium upon return. | 2010 | 19821128 |
| interactions and oligomerization of hantavirus glycoproteins. | in this report the basis for the structural architecture of the envelope of hantaviruses, family bunyaviridae, is systematically studied by the interactions of two glycoproteins n and c (gn and gc, respectively) and their respective disulfide bridge-mediated homo- and heteromeric oligomerizations. in virion extracts gn and gc associated in both homo- and hetero-oligomers which were, at least partially, thiol bridge mediated. due to strong homo-oligomerization, the hetero-oligomers of gn and gc a ... | 2010 | 19828613 |
| molecular diagnostics of hemorrhagic fever with renal syndrome during a dobrava virus infection outbreak in the european part of russia. | a large outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred in the winter of 2006-2007 in a region southeast of moscow in central european russia. of the 422 patients with hfrs investigated in this study, 58 patients were found to be infected by puumala virus, whereas as many as 364 were infected by dobrava-belgrade virus (dobv). early serum samples from 10 dobv-infected patients were used for nucleic acid amplification, which was successful for 5 patients. molecular analyses demon ... | 2009 | 19828747 |
| genetic detection of dobrava/belgrade virus in a czech patient with haemorrhagic fever with renal syndrome. | in the summer of 2008, a 15-year-old boy was hospitalized in a paediatric intensive care unit in the czech republic. laboratory diagnosis of hantavirus infection was established by serological and molecular methods. sequence and phylogenetic analyses showed that the causative strain was dobrava/belgrade virus, which is genetically closer to strains associated with apodemus flavicollis rodents. | 2010 | 19832712 |
| vaccines for hantaviruses. | hantaviruses are assigned to categories a and c on the national institute of allergy and infectious diseases (niaid) priority pathogens list and to category c on the centers for disease control and prevention (cdc) emergency preparedness and response list of boterrorism agents/diseases. these rodent-borne viruses are members of the family bunyaviridae and are transmitted to humans in aerosols of rodent excreta. there are more than 20 recognized hantaviruses, some of which are associated with one ... | 2009 | 19837289 |
| andes virus recognition of human and syrian hamster beta3 integrins is determined by an l33p substitution in the psi domain. | andes virus (andv) causes a fatal hantavirus pulmonary syndrome (hps) in humans and syrian hamsters. human alpha(v)beta(3) integrins are receptors for several pathogenic hantaviruses, and the function of alpha(v)beta(3) integrins on endothelial cells suggests a role for alpha(v)beta(3) in hantavirus directed vascular permeability. we determined here that andv infection of human endothelial cells or syrian hamster-derived bhk-21 cells was selectively inhibited by the high-affinity alpha(v)beta(3) ... | 2010 | 19846530 |
| domestically acquired seoul virus causing hemorrhagic fever with renal syndrome-maryland, 2008. | hantaviruses are rodent-borne viruses capable of causing human disease. the seoul virus is a hantavirus that causes hemorrhagic fever with renal syndrome in east asia. to our knowledge, we report the first domestically acquired case of hemorrhagic fever with renal syndrome caused by the seoul virus, confirmed by serology testing, reverse-transcriptase polymerase chain reaction, and nucleotide sequence analysis. the patient presented with myalgias and fever, and developed acute renal failure. | 2009 | 19848600 |
| co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary. | hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ... | 2009 | 19856478 |
| [infectious diseases and climate change]. | climate changes will likely have an impact on the spectrum of infectious diseases in europe. we may see an increase in vector-borne diseases, diseases spread by rodents such as hantavirus, and food- and water-borne diseases. as the effects of climate changes are likely to occur gradually, a modern industrialised country such as denmark will have the opportunity to adapt to the expected changes. | 2009 | 19857396 |
| ecology of hantaviruses and their hosts in north america. | since the 1993 discovery of a highly pathogenic hantavirus associated with the north american deer mouse (peromyscus maniculatus), intensive ecological studies have led to many advances in our understanding of the natural history of new world hantaviruses as it relates to human disease. seventeen named hantaviruses have been identified in north america. field and laboratory studies of sin nombre and other hantaviruses have delineated host associations, geographical distributions, mechanisms of t ... | 2010 | 19874190 |
| puumala virus reference strain for hantavirus serodiagnosis in france. | | 2010 | 19885688 |
| molecular epidemiological and serological studies of hantavirus infection in northern vietnam. | the distribution of anti-hantavirus antibodies in humans and rodents in northern vietnam was examined. in total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for igg antibodies against hantaan or seoul virus (seov) by elisa, ifa, and western blot analysis. antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of hai phong, and 2/185 residents of ha nam province. in comparison ... | 2009 | 19887743 |
| extensive host sharing of central european tula virus. | to examine the host association of tula virus (tulv), a hantavirus present in large parts of europe, we investigated a total of 791 rodents representing 469 microtus arvalis and 322 microtus agrestis animals from northeast, northwest, and southeast germany, including geographical regions with sympatric occurrence of both vole species, for the presence of tulv infections. based on serological investigation, reverse transcriptase pcr, and subsequent sequence analysis of partial small (s) and mediu ... | 2010 | 19889769 |
| seasonal dispersal patterns of sylvan deer mice (peromyscus maniculatus) within montana rangelands. | we examined seasonal dispersal patterns and timing of new infections of sin nombre virus (snv), as determined by recent acquisition of antibodies (seroconversion), in deer mice (peromyscus maniculatus) at two montana rangeland study sites over three years, 2004-2007. one study site was located in grassland habitat, and the other was located in shrub-steppe. in montana, both of these habitats are commonly associated with peridomestic environments (in and around buildings). peridomestic environmen ... | 2009 | 19901376 |
| study of andes virus entry and neutralization using a pseudovirion system. | andes virus (andv), a member of the hantavirus genus in the family bunyaviridae, causes an acute disease characteristic of new-world hantaviruses called hantavirus pulmonary syndrome (hps). hps is a highly pathogenic disease with a case-fatality rate of 40%. andv is the only hantavirus reported to spread directly from human-to-human. the aim of the present study was to develop a quantitative and high-throughput pseudovirion assay to study andv infection and neutralization in biosafety level 2 fa ... | 2010 | 19903496 |
| reappearance of viral hemorrhagic fever with renal syndrome in northwestern greece. | minor outbreaks of viral hemorrhagic fever (vhf) with renal failure have occurred in northwestern greece over past decades. however, during the most recent decade, there has been a paucity of human cases despite the detection of hantavirus in rodents of this area. | 2010 | 19926507 |
| genetic diversity and phylogeography of seewis virus in the eurasian common shrew in finland and hungary. | recent identification of a newfound hantavirus, designated seewis virus (swsv), in the eurasian common shrew (sorex araneus), captured in switzerland, corroborates decades-old reports of hantaviral antigens in this shrew species from russia. to ascertain the spatial or geographic variation of swsv, archival liver tissues from 88 eurasian common shrews, trapped in finland in 1982 and in hungary during 1997, 1999 and 2000, were analyzed for hantavirus rnas by reverse transcription-polymerase chain ... | 2009 | 19930716 |
| a serosurvey reveals bosnia and herzegovina as a europe's hotspot in hantavirus seroprevalence. | the extent of hantavirus seroprevalence in the healthy population from bosnia and herzegovina has not yet been investigated; therefore, the aim of this study was to assess the hantavirus seroprevalence in the population from different regions of bosnia and herzegovina and in different risk groups. the serosurvey included 1331 subjects from endemic and non-endemic regions in bosnia and herzegovina. all sera samples were examined using igg elisa, and western blot (bunyavirus igg) tests. hantavirus ... | 2010 | 19951455 |
| hsp70 induced by hantavirus infection interacts with viral nucleocapsid protein and its overexpression suppresses virus infection in vero e6 cells. | hantavirus (htv) infection is known to induce innate cellular response, a more specified cellular response in the host cells. however, whether it stimulates synthesis of stress proteins, particularly associations of viral proteins, is entirely unknown. the primary focus of this research is using vero e6 cells infected with hantaan 76-118 (htnv) as an in vitro infection model to examine the individual contribution of htv infection to heat shock response. this study shows that htnv infection rapid ... | 2009 | 19956449 |
| tula hantavirus nss protein accumulates in the perinuclear area in infected and transfected cells. | the small rna segment of some hantaviruses (family bunyaviridae) encodes two proteins: the nucleocapsid protein and, in an overlapping reading frame, a non-structural (nss) protein. the hantavirus nss protein, like those of orthobunya- and phleboviruses, counteracts host innate immunity. here, for the first time, the nss protein of a hantavirus (tula virus) has been observed in infected cells and shown to localize in the perinuclear area. transiently expressed nss protein showed similar localiza ... | 2010 | 19956987 |
| [research on hantanvirus infection of rodents in junan county, shandong province]. | | 2009 | 19957614 |
| sympatry of 2 hantavirus strains, paraguay, 2003-2007. | to explore geographic and host-taxonomic patterns of hantaviruses in paraguay, we established sampling sites in the mbaracayu biosphere reserve. we detected jabora virus and itapua37/juquitiba-related virus in locations approximately 20 m apart in different years, which suggested sympatry of 2 distinct hantaviruses. | 2009 | 19961679 |
| cross-sectional survey of hantavirus infection, brazil. | a cross-sectional serosurvey was conducted to assess the proportion of persons exposed to hantaviruses in a virus-endemic area of the state of minas gerais, brazil. findings of this study suggested the presence of > or =1 hantaviruses circulating in this region causing hantavirus pulmonary syndrome, mild disease, or asymptomatic infection. | 2009 | 19961680 |
| dobrava-belgrade virus spillover infections, germany. | we present the molecular identification of apodemus agrarius (striped field mouse) as reservoir host of the dobrava-belgrade virus (dobv) lineage dobv-aa in 3 federal states of germany. phylogenetic analyses provided evidence for multiple spillover of dobv-aa to a. flavicollis, a crucial prerequisite for host switch and genetic reassortment. | 2009 | 19961690 |
| hantavirus regulation of endothelial cell functions. | hantaviruses cause two vascular permeability-based diseases and primarily infect endothelial cells which form the primary fluid barrier of the vasculature. since hantavirus infections are not lytic, the mechanisms by which hantaviruses cause haemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps) are indeterminate. hps is associated with acute pulmonary oedema and hfrs with moderate haemorrhage and renal sequelae, perhaps reflecting the location of vast microvascular ... | 2009 | 19967132 |
| [thrombocytopenia and acute kidney injury]. | a 31-year old man is addressed to the emergency department for fever, abdominal pain and vomiting. laboratory tests reveal an inflammatory syndrome, thrombocytopenia, acute kidney injury associated with major proteinuria (more than 3 g/24 h). evaluation will conclude to an acute interstitial nephritis and the serology is positive for hantavirus. this clinical case illustrates differential diagnosis of acute renal failure and reminds the theory of hemorrhagic fever with renal syndrome : usual cli ... | 2009 | 19998799 |
| [from mice to men: the threat of hantavirus infections]. | hantaviruses are naturally hosted by small mammals. virus transmission to humans can result in the development of hemorrhagic fever with renal syndrome (asia, europe) or hantavirus cardiopulmonary syndrome (the americas). certain hantaviruses can cause life-treatening infections with case fatality indices up to about 50%. different virus types have evolved which use different species of small mammals (mainly but not exclusively rodent species) as natural reservoirs. it is still unknown why these ... | 2009 | 19999378 |
| production and characterization of a recombinant single-chain antibody against hantaan virus envelop glycoprotein. | hantaan virus (htnv) is the type of hantavirus causing hemorrhagic fever with renal syndrome, for which no specific therapeutics are available so far. cell type-specific internalizing antibodies can be used to deliver therapeutics intracellularly to target cell and thus, have potential application in anti-htnv infection. to achieve intracellular delivery of therapeutics, it is necessary to obtain antibodies that demonstrate sufficient cell type-specific binding, internalizing, and desired cellul ... | 2010 | 20012277 |
| the effect of seasonality, density and climate on the population dynamics of montana deer mice, important reservoir hosts for sin nombre hantavirus. | 1. since sin nombre virus was discovered in the u.s. in 1993, longitudinal studies of the rodent reservoir host, the deer mouse (peromyscus maniculatus) have demonstrated a qualitative correlation among mouse population dynamics and risk of hantavirus pulmonary syndrome (hps) in humans, indicating the importance of understanding deer mouse population dynamics for evaluating risk of hps. 2. using capture-mark-recapture statistical methods on a 15-year data set from montana, we estimated deer mous ... | 2010 | 20015212 |
| hantavirus pulmonary syndrome in five pediatric patients - four states, 2009. | hantavirus pulmonary syndrome (hps) is a reportable infectious disease with a high case-fatality rate, transmitted to humans by exposure to rodents. each year, 20--40 cases of hps occur in the united states; cases in persons aged <17 years make up fewer than 7% of those cases, and cases in children aged <10 years are exceptionally rare. cdc received reports of five pediatric cases of hps occurring during may 16--november 25, 2009, among children aged 6--14 years from arizona, california, colorad ... | 2009 | 20032925 |
| epidemiological study of hantavirus infection in the samara region of european russia. | european russia is a highly endemic area of hemorrhagic fever with renal syndrome (hfrs), a rodent-borne zoonotic disease, caused by hantaviruses. in total, 145 small mammals of four species (myodes glareolus, apodemus flavicollis, a. agrarius, and a. uralensis) were trapped in the samara region of european russia in august 2005 and examined for the presence of hantavirus (hv). anti-hv antibodies were found in six of 68 (8.8%) m. glareolus and in one of 19 (5.3%) a. flavicollis by indirect immun ... | 2009 | 20046023 |
| milder winters in northern scandinavia may contribute to larger outbreaks of haemorrhagic fever virus. | the spread of zoonotic infectious diseases may increase due to climate factors such as temperature, humidity and precipitation. this is also true for hantaviruses, which are globally spread haemorrhagic fever viruses carried by rodents. hantaviruses are frequently transmitted to humans all over the world and regarded as emerging viral diseases. climate variations affect the rodent reservoir populations and rodent population peaks coincide with increased number of human cases of hantavirus infect ... | 2009 | 20052429 |
| [geographic expansion of hantavirus pulmonary syndrome in argentina. the southernest case report]. | since 1995 more than 1000 cases of hantavirus pulmonary syndrome (hps) were reported in argentina, a severe disease and often fatal to humans. most cases were associated with andes virus (and) that caused few events of person-to-person transmission. several lineages of pathogenic and viruses have been described, including and south, hosted by the rodent oligoryzomys longicaudatus which affects the patagonian region of argentina and chile. we studied the clinical and epidemiological characteristi ... | 2009 | 20053606 |
| natural host relationships of hantaviruses native to western venezuela. | strains of caño delgadito virus (cadv) and maporal virus (mapv) were isolated from 25 (8.9%) of the 280 rodents captured on farms in 1997 in western venezuela. the results of analyses of laboratory and zoographic data indicated that alston's cotton rat (sigmodon alstoni) is the principal host of cadv, horizontal virus transmission is the dominant mode of cadv transmission in alston's cotton rat in nature, a pygmy rice rat (oligoryzomys sp.) is the principal host of mapv, and the natural host rel ... | 2010 | 20055578 |
| infection of in vivo differentiated human mast cells with hantaviruses. | increased vascular permeability is a key feature of the pathological symptoms caused by hantaviruses. here, we analysed the interaction between hantaviruses and mast cells, which regulate vascular homeostasis. in highly purified human skin mast cells increasing amounts of hantaan (htnv) and, to a lower extent, prospect hill (phv) virions were produced. replication was confirmed by the production of viral plus-strand rna as determined by a virus strand-specific rt-pcr. phv but not htnv elicited e ... | 2010 | 20071485 |
| epidemic viral pneumonia. | two recent viral epidemics producing pneumonitis (severe acute respiratory syndrome and pandemic influenza a h1n1) have highlighted the potential for viral infections to cause respiratory failure with a significant risk of mortality. this review describes these epidemics and other causes of epidemic viral pneumonia. | 2010 | 20087179 |
| perspectives on scrub typhus, tick-borne pathogens, and hantavirus in the republic of korea. | | 2009 | 20088033 |
| molecular characterization of two hantavirus strains from different rattus species in singapore. | hantaviruses cause human disease in endemic regions around the world. outbreaks of hantaviral diseases have been associated with changes in rodent population density and adaptation to human settlements leading to their proliferation in close proximity to human dwellings. in a parallel study initiated to determine the prevalence of pathogens in singapore's wild rodent population, 1206 rodents were trapped and screened. the findings established a hantavirus seroprevalence of 34%. this paper descri ... | 2010 | 20096099 |
| [genotyping and sequence analysis on g2 segment of hantavirus carried by rat in hebei province]. | to know the genotype and subtype of hantavirus (hv) carried by host animals in hfrs natural epidemic area of hebei province. | 2009 | 20104766 |
| models for the spread and persistence of hantavirus infection in rodents with direct and indirect transmission. | hantavirus, a zoonotic disease carried by wild rodents, is spread among rodents via direct contact and indirectly via infected rodent excreta in the soil. spillover to humans is primarily via the indirect route through inhalation of aerosolized viral particles. rodent-hantavirus models that include direct and indirect transmission and periodically varying demographic and epidemiological parameters are studied in this investigation. two models are analyzed, a nonautonomous system of differential ... | 2010 | 20104956 |
| puumala hantavirus and myodes glareolus in northern europe: no evidence of co-divergence between genetic lineages of virus and host. | the genus hantavirus (family bunyaviridae) includes negative-strand rna viruses that are carried by persistently infected rodent and insectivore species. puumala virus (puuv), carried by bank voles (myodes glareolus), is a pathogenic hantavirus that causes outbreaks of mild haemorrhagic fever with renal syndrome across europe. in northern europe, puuv is represented by several genetic lineages that are maintained by distinct phylogroups of bank voles. the present study describes sequences of new ... | 2010 | 20107019 |
| one-step rna pathogen detection with reverse transcriptase activity of a mutated thermostable thermus aquaticus dna polymerase. | we describe the cloning and characterization of a mutated thermostable dna polymerase from thermus aquaticus (taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one-step pcr pathogen detection using established real-time detection methods. we demonstrate that this taq polymerase mutant (taq m1) has similar pcr sensitivity and nuclease activity as the respective taq wild-type dna polymerase. in addition, and in marked contrast to the wild-type, taq m1 e ... | 2010 | 20108275 |