| crimean-congo haemorrhagic fever presenting as thrombotic microangiopathy and acute renal failure. | | 2006 | 16735392 |
| [investigation on hantaviruses infection in rodents from free markets in beijing areas]. | in order to find out the factors related to hemorrhagic fever with renal syndrome (hfrs) infection, and to evaluate the probability of ecdemic hantaviruses (hv) infection in rodents in beijing areas. | 2006 | 16749997 |
| predicting the emergence of human hantavirus disease using a combination of viral dynamics and rodent demographic patterns. | the paper proposes a model explaining the spatial variation in incidence of nephropathia epidemica in europe. we take into account the rodent dynamic features and the replicative dynamics of the virus in animals, high in the acute phase of newly infected animals and low in the subsequent chronic phase. the model revealed that only vole populations with multi-annual fluctuations allow for simultaneously high numbers of infected rodents and high proportions of those rodents in the acute excretion ... | 2007 | 16753079 |
| phylogeography of the deer mouse (peromyscus maniculatus) provides a predictive framework for research on hantaviruses. | phylogeographical partitioning of sin nombre and monongahela viruses (hantaviruses) may reflect that of their primary rodent host, the deer mouse (peromyscus maniculatus). lack of a comprehensive assessment of phylogeographical variation of the host has precluded the possibility of predicting spatial limits of existing strains of these viruses or geographical regions where novel viral strains might emerge. the complete cytochrome b gene was sequenced for 206 deer mice collected from sites throug ... | 2006 | 16760402 |
| modeling hantavirus reservoir species dominance in high seroprevalence areas on the azuero peninsula of panama. | habitat fragmentation commonly influences distribution of zoonotic disease reservoirs. in panama, populations of rodent hosts of hantaviruses are favored by small habitat fragments isolated by agricultural lands. we expected a similar relationship between landscape characteristics and host distribution at fine geographical scales in southern panama. the relative abundance of zygodontomys brevicauda, the primary host for "calabazo" virus, and other rodents was assessed at 24 sites within the azue ... | 2006 | 16760528 |
| hantavirus pulmonary syndrome--five states, 2006. | hantavirus pulmonary syndrome (hps) is a rodentborne viral disease characterized by severe pulmonary illness and a case-fatality ratio of 30%-40%. sin nombre virus causes the majority of hps cases in the united states, and the deer mouse (peromyscus maniculatus) is its predominant reservoir. this report describes an increase in human cases of hps reported during january-march 2006 from arizona, new mexico, north dakota, texas, and washington state. the findings emphasize the need for renewed att ... | 2006 | 16760891 |
| characterization of the rna chaperone activity of hantavirus nucleocapsid protein. | hantaviruses are tripartite negative-sense rna viruses and members of the bunyaviridae family. the nucleocapsid (n) protein, encoded by the smallest of the three genome segments (s), has nonspecific rna chaperone activity. this activity results in transient dissociation of misfolded rna structures, may be required for facilitating correct higher-order rna structure, and may function in viral genome replication. we carried out a series of experiments to further characterize the ability of n to di ... | 2006 | 16775315 |
| laboratory study on the immobilization of bacterial spores in arid environments. | in this study, two effective, non-toxic, wind erosion palliative materials were analyzed for their efficacy in preventing the spread of bacterial spores. desert sand was employed in a laboratory setting with a non-toxic simulant bacterium in an attempt to accurately represent the spreadability of the hantavirus. spore simulants were used instead of viruses due to availability, decreased susceptibility to desiccation and detection ability without involving tissue cultures. the simulant was used t ... | 2006 | 16779932 |
| larger than usual increase in cases of hantavirus infections in belgium, france and germany, june 2005. | | 2005 | 16785666 |
| increased hantavirus infections in luxembourg, august 2005. | | 2005 | 16785690 |
| [study on the association between hantavirus infection and rattus norvegicus]. | to further understand the association of hantavirus (hv) harbored and transmitted in wild brown rats. | 2006 | 16792882 |
| hantavirus cardiopulmonary syndrome: immune response and pathogenesis. | hantaviruses are emerging viruses in the americas that cause cardiopulmonary syndrome with high lethality. the intense cellular immune response to hantavirus alters normal endothelial cell barrier functions and seems to be harmful to the host. on the other hand, the humoral immune response seems to be essential for recovery from infection. | 2006 | 16793309 |
| mathematical models for hantavirus infection in rodents. | hantavirus pulmonary syndrome is an emerging disease of humans that is carried by wild rodents. humans are usually exposed to the virus through geographically isolated outbreaks. the driving forces behind these outbreaks is poorly understood. certainly, one key driver of the emergence of these viruses is the virus population dynamics within the rodent population. two new mathematical models for hantavirus infection in rodents are formulated and studied. the new models include the dynamics of sus ... | 2006 | 16794943 |
| a history of biological disasters of animal origin in north america. | this paper examines past occurrences in north america relevant to the possibility of biological disasters with animal origins. with respect to naturally occurring animal disease outbreaks, north america, while not as adversely affected by epizootics as other regions, has had its fair share of such outbreaks of both 'traditional' and emerging animal diseases. the traditional category includes such diseases as anthrax, classical swine fever, bluetongue, brucellosis, foot and mouth disease, and the ... | 2006 | 16796038 |
| genetic analysis of puumala hantavirus strains from austria. | sequences of the small (s) and the medium (m) genome segments of puumala hantavirus (puuv) were recovered from bank voles clethrionomys glareolus trapped at 2 locations, klippitztörl (carinthia) and ernstbrunn (lower austria). lung tissue samples from 12 rodents earlier found hantavirus antibody-positive were further screened for the presence of hantaviral n-antigen using immunoblotting. rna purified from 7 n-ag-positive samples was subjected to the reverse transcription-polymerase chain reactio ... | 2006 | 16798703 |
| metabolic activity of macrophages infected with hantavirus, an agent of hemorrhagic fever with renal syndrome. | monocytes/macrophages are thought to play an important role in pathogenesis of viral infections. these cells are involved in distribution and persistence of viruses in the organism and also influence the regulation of immune reactions. the functional and enzymatic activities of macrophages infected with an agent of hemorrhagic fever with renal syndrome were analyzed for the first time. this disease is caused by a virus of the hantavirus genus, the bunyaviridae family. activities of ectoenzymes 5 ... | 2005 | 16266269 |
| [full length nucleotide sequence analysis of l and m segments of hantavirus q32 strain gene]. | to study the full length l and m sequence of hantavirus q32 strain gene and explore its molecular characters. | 2006 | 16816865 |
| [two patients with hantavirus infection in the netherlands; substantial increase in incidence in neighbouring countries]. | a 12-year-old girl and a 57-year-old woman were admitted with fever, general malaise, abdominal pain, nausea and vomiting. both patients had acute renal insufficiency based on tubulointerstitial nephritis caused by the genus hantavirus, which was confirmed by blood tests. both patients recovered spontaneously. the neighbouring countries of france, germany and belgium have recently reported 2- to 7-fold increases in the number of hantavirus infections. hantavirus is a zoonotic viral disease that ... | 2006 | 16821456 |
| [study of phylogenetic relations of hantaviruses by using the mutual information method. tula genotype viruses]. | phylogenetic relations were studied in the group of tula viruses (hantavirus genus, bunyaviridae family) of eurasia. the similarity in the nucleotide sequences of segments was estimated by the value of mutual information. the application of this method confirmed that recombinant events might occur in some tula-like viruses of eurasia, as previously presumed by the results of bootscanning and analysis of amino acid sequences. it was found that might be recombination between distinct phylogenetic ... | 2006 | 16826755 |
| reversible impairment of myocardial function in hanta virus infection - direct viral effect? | | 2006 | 16845572 |
| human hantavirus infection, brazilian amazon. | | 2006 | 16845782 |
| prolonged survival of puumala hantavirus outside the host: evidence for indirect transmission via the environment. | the capability of rodent-borne viruses to survive outside the host is critical for the transmission dynamics within rodent populations and to humans. the transmission of puumala virus (puuv) in colonized bank voles (clethrionomys glareolus) was investigated and additional longevity studies in cell culture with puuv and tula (tulv) hantaviruses were performed. wild-type puuv excreted by experimentally infected donor bank voles was shown to be transmitted indirectly between rodents through contami ... | 2006 | 16847107 |
| loss of cell membrane integrity in puumala hantavirus-infected patients correlates with levels of epithelial cell apoptosis and perforin. | hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome are two diseases caused by hantaviruses. capillary leakage is a hallmark of hantavirus infection. pathogenic hantaviruses are not cytotoxic, but elevated levels of serum lactate dehydrogenase (ldh), indicative of cellular damage, are observed in patients. we report increased levels of serum perforin, granzyme b, and the epithelial cell apoptosis marker caspase-cleaved cytokeratin-18 during puumala hantavirus infection. signi ... | 2006 | 16873286 |
| drotrecogin alpha (activated) in two patients with the hantavirus cardiopulmonary syndrome. | hantavirus cardiopulmonary syndrome (hcps) is associated with rapid cardiopulmonary collapse from endothelial injury, resulting in massive capillary leak, shock and severe hypoxemic respiratory failure. to date, treatment remains supportive and includes mechanical ventilation, vasopressors and extracorporeal membrane oxygenation, with mortality approaching 50%. two hcps survivors initially given drotrecogin alpha (activated) (daa) for presumed bacterial septic shock are described. vasoactive med ... | 2006 | 16896430 |
| clinical and laboratory findings related to a favorable evolution of hantavirus pulmonary syndrome. | the medical records of 27 patients with hantavirus pulmonary syndrome were analyzed according to the need for invasive mechanical ventilation in relation to the following data up on hospital admission: age, gender, fever, cough, dyspnea, systolic arterial blood pressure, heart rate, levels of hemoglobin, hematocrit, leukocytes, lymphocytes, platelets, creatinine and arterial blood gases. the volume infused during the first 24 hours after admission, the use of inotropic agents, the use of cortico ... | 2006 | 16906244 |
| [a review about nephropathia epidemica]. | we propose a review of history, aetiology, physiopathology, clinical features, treatment and prevention of nephropathia epidemica (ne) which represents the only form of hantavirus infection in belgium. | 2006 | 16910256 |
| hemorrhagic fever with renal syndrome complicated with pregnancy: a case report. | hantaviruses cause two forms of human disease: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome. hantavirus infection can occur in pregnant women and it can have an influence on the maternal and fetal outcomes, although this is a rare finding even in endemic areas. we describe here a recent case of hfrs complicating pregnancy. | 2006 | 16913449 |
| a pseudotype vesicular stomatitis virus containing hantaan virus envelope glycoproteins g1 and g2 as an alternative to hantavirus vaccine in mice. | we examined whether a vesicular stomatitis virus (vsv) pseudotype bearing the hantavirus envelope glycoproteins (gps) g1 and g2 (vsvdeltag*htn) could be used as a safe and effective alternative to native hantavirus. mice were immunized with purified particles of vsvdeltag*htn. after the second immunization, all mice produced anti-gp antibody as detected in elisa and a neutralization test. after the third immunization, the mice were challenged with hantaan virus. neither anti-np antibody producti ... | 2006 | 16426712 |
| [first serological evidence of hantavirus infection in rodents in colombia]. | determining hantavirus infection prevalence in rodents in the c6rdoba department, colombia. | 2006 | 16925117 |
| genetic properties of medium (m) and small (s) genomic rna segments of seoul hantavirus isolated from rattus norvegicus and antigenicity analysis of recombinant nucleocapsid protein. | a novel isolate of seoul (seo) hantaviruses was detected and identified in rattus norvegicus in shandong province, china and designated as jun5-14. the partial m segment and the coding region of nucleocapsid protein (np) in the s segment of jun5-14 were pcr-amplified and sequenced. nucleotide sequence analysis of the partial m segment (300 bp) revealed that jun5-14 isolate was closely related to former seo isolates in shandong (97.3-99.0% homology) and non-shandong seo viruses (84.1-97.7% homolo ... | 2007 | 16927115 |
| clinical and laboratory predictors of oliguric renal failure in haemorrhagic fever with renal syndrome caused by hantaan virus. | haemorrhagic fever with renal syndrome (hfrs), caused by hantavirus infection, develops into acute renal failure (arf) of variable degrees of severity. we investigated the early predictive markers for oliguric arf in hfrs patients. | 2007 | 16930718 |
| the changing epidemiology of acute renal failure. | different definitions of acute renal failure (arf) abound. the existence of multiple definitions makes it difficult to determine the true epidemiological characteristics of this condition. despite this difficulty, it has been possible to detect notable variations in the epidemiology of arf during the past few decades. the absolute incidence of arf has increased, while associated mortality rate has remained relatively static. several factors have contributed to this altered epidemiology. here, we ... | 2006 | 16932465 |
| hsp70 gene fused with hantavirus s segment dna significantly enhances the dna vaccine potency against hantaviral nucleocapsid protein in vivo. | heat shock proteins (hsps) have been shown to act as adjuvants when coadministered with peptide antigens or given as fusion proteins and enhance the vaccination efficiency. to evaluate the enhancement of the potency of hantaan virus (htnv) nucleocapsid protein (np) immunogenicity by heat shock protein 70 (hsp70), we developed a novel chimeric htnv s-hsp70 dna vaccine plasmid by genetically linking hsp70 gene to the full-length htnv s segment dna (htnv s dna). c57bl/6 mice were immunized with thi ... | 2007 | 16935395 |
| oligomerization of hantavirus nucleocapsid protein: analysis of the n-terminal coiled-coil domain. | hantaviruses constitute a genus in the family bunyaviridae. they are enveloped negative-strand rna viruses with a tripartite genome encoding the nucleocapsid (n) protein, the two surface glycoproteins gn and gc, and an rna-dependent rna polymerase. the n protein is the most abundant component of the virion; it encapsidates genomic rna segments forming ribonucleoproteins and participates in genome transcription and replication as well as virus assembly. in the course of rna encapsidation, n prote ... | 2006 | 16940519 |
| diagnosis and treatment of new world hantavirus infections. | the purpose of this review is to summarize the current knowledge regarding the diagnosis and treatment of indigenous new world hantavirus infections. | 2006 | 16940866 |
| emerging and re-emerging viral infections in europe. | emerging viral infections are becoming a serious problem in europe in the recent years. this is particularly true for severe acute respiratory syndrome (sars), west nile virus (wnv) disease, toscana virus (tosv) disease, and potentially for avian influenza virus (h5n1). in contrast, emergence or re-emergence of severe viral infections, including tick borne encephalitis virus, and viral haemorrhagic fever caused by hantavirus and dengue virus have been frequently reported in several european coun ... | 2007 | 16944546 |
| tropical infections in the icu. | certain arthropod-borne infections are common in tropical regions because of favorable climatic conditions. water-borne infections like leptospirosis are common due to contamination of water especially during the monsoon floods. infections like malaria, leptospirosis, dengue fever and typhus sometimes cause life threatening organ dysfunction and have several overlapping features. most patients present with classicial clinical syndromes: fever and thrombocytopenia are common in dengue, malaria an ... | 2006 | 16944613 |
| characterization of shock in a hamster model of hantavirus infection. | human hantavirus cardiopulmonary syndrome (hcps) due to andes, sin nombre and other hantaviruses is characterized by severe pulmonary capillary leak and cardiogenic shock. hamsters, the only animal manifesting hcps-like disease, were instrumented with radiotelemeters that enabled ambulatory intracarotid blood pressure recording within an animal biosafety level-4 facility. following infection with andes virus, blood pressure and heart rate decreased slowly in a biphasic manner during the first 7 ... | 2006 | 16950493 |
| genetic analysis of thailand hantavirus in bandicota indica trapped in thailand. | sixty one tissue samples from several rodent species trapped in five provinces of thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. four samples, all from the great bandicoot rat bandicota indica, were confirmed positive for the hantaviral n-antigen. two of them were trapped in nakhon pathom province, the other two in nakhon ratchasima province, approximately 250 km from the other trapping site. when analysed by rt-nested pcr, all four rodent ... | 2006 | 16953877 |
| nitric oxide and peroxynitrite have different antiviral effects against hantavirus replication and free mature virions. | reactive nitrogen intermediates (rni), like nitric oxide (no) and peroxynitrite, have antiviral effects against certain viruses. hantaviruses, like other members of the bunyaviridae family, have previously not been shown to be sensitive to rni. in this study, we compared the effects of no and peroxynitrite on hantavirus replication and free mature virions in vitro, and of inducible nitric oxide synthase (inos) in hantavirus-infected suckling mice. the no-generating compound s-nitroso-n-acetylpen ... | 2006 | 16955520 |
| incubation period of hantavirus cardiopulmonary syndrome. | the potential incubation period from exposure to onset of symptoms was 7-39 days (median 18 days) in 20 patients with a defined period of exposure to andes virus in a high-risk area. this period was 14-32 days (median 18 days) in 11 patients with exposure for <48 hours. | 2006 | 16965713 |
| hantavirus n protein exhibits genus-specific recognition of the viral rna panhandle. | a key genomic characteristic that helps define hantavirus as a genus of the family bunyaviridae is the presence of distinctive terminal complementary nucleotides that promote the folding of the viral genomic segments into "panhandle" hairpin structures. the hantavirus nucleocapsid protein (n protein), which is encoded by the smallest of the three negative-sense genomic rna segments, undergoes in vivo and in vitro trimerization. trimeric hantavirus n protein specifically recognizes the panhandle ... | 2006 | 16971445 |
| regions of importance for interaction of puumala virus nucleocapsid subunits. | puumala virus (puuv) is a hantavirus that causes a mild form of hemorrhagic fever with renal syndrome in northern and central europe, and in large parts of russia. the nucleocapsid (n) protein encoded by hantaviruses plays an important role in the life-cycle of these viruses, and one important function for the n-protein is to oligomerize, surround and protect the viral rnas. we have identified amino- and carboxy-terminal regions involved in puuv n-n interactions, which comprise amino acids 100-1 ... | 2006 | 16972031 |
| characterization of a new puumala virus genotype associated with hemorrhagic fever with renal syndrome. | hantavirus serotype puumala (puuv) is the etiologic agent of nephropathia epidemica (ne), a mild variant of hemorrhagic fever with renal syndrome (hfrs) in europe, with lethality rates up to 1% among human patients. the serotype puuv is composed of numerous geographically restricted strains forming a great number of phylogeographic lineages, sublineages, variants, and clusters. we describe a new, geographically and phylogenetically well-defined pathogenic puuv sublineage in southwest germany (st ... | 2006 | 16972039 |
| new world hantavirus in humans, french guiana. | | 2006 | 16972355 |
| the pathogenic ny-1 hantavirus g1 cytoplasmic tail inhibits rig-i- and tbk-1-directed interferon responses. | hantaviruses cause two diseases with prominent vascular permeability defects, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. all hantaviruses infect human endothelial cells, although it is unclear what differentiates pathogenic from nonpathogenic hantaviruses. we observed dramatic differences in interferon-specific transcriptional responses between pathogenic and nonpathogenic hantaviruses at 1 day postinfection, suggesting that hantavirus pathogenesis may in part be de ... | 2006 | 16973572 |
| truncated recombinant dobrava hantavirus nucleocapsid proteins induce strong, long-lasting immune responses in mice. | we describe the cloning and expression of dobrava hantavirus (dobv) nucleocapsid proteins and a truncated form consisting of the first 118 n-terminal amino acids, and the capacity of these e. coli icone 200-expressed recombinant proteins (rnp) to induce a protective immune response against dobv in mice. as an alternative carrier protein, the outer membrane protein a derived from klebsiella pneumoniae (rp40) has been coupled to different rnp constructs. all recombinant proteins were found to be h ... | 2006 | 16714853 |
| increased permeability of human endothelial cell line ea.hy926 induced by hantavirus-specific cytotoxic t lymphocytes. | hantavirus infection causes two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. the typical feature of these diseases is increased permeability in microvascular beds in the kidneys and the lungs, respectively. the mechanism of capillary leakage, however, is not understood. some evidence suggests that hantavirus disease pathogenesis is immunologically mediated by cytotoxic t lymphocytes and other immune cells in target organs producing inflammatory cytokin ... | 2007 | 16979772 |
| emergency medicine and the public's health: emerging infectious diseases. | in recent years, multiple global forces have contributed to the emergence and widespread distribution of previously unknown disease entities. this article discusses ebola virus, west nile virus, and hantavirus as representative emerging infectious diseases. smallpox is discussed along with concerns about the safety of the smallpox vaccine, given the uncertain risk of bioterrorism and smallpox exposure. ed physicians must become familiar with the presentation, management, and public health impact ... | 2006 | 16982350 |
| tumor necrosis factor-alpha genetic predisposing factors can influence clinical severity in nephropathia epidemica. | severe human infection with hantavirus is characterized by high fever, cold chills, thrombocytopenia, arterial hypotension, acute renal failure, and/or adult respiratory distress syndrome (ards)-like pulmonary involvement, but the clinical course varies greatly between individuals. we investigated whether genetically determined differences in tumor necrosis factor (tnf)-alpha production can influence the severity of hantavirus disease. we studied a tnf-alpha single-nucleotide promoter polymorphi ... | 2006 | 16987073 |
| puumala hantavirus in slovenia: analyses of s and m segment sequences recovered from patients and rodents. | in slovenia, the co-existence of dobrava and puumala (puuv) hantaviruses in a single endemic region has been demonstrated. this study presents selected slovenian hfrs cases caused by puuv combined with genetic analysis of viral genome sequences recovered from clinical specimens and tissue samples of clethrionomys glareolus (bank voles). serum samples from nine hfrs patients were included in the study. rodents study sites were selected with regard to the hfrs cases. partial s segment sequences we ... | 2007 | 16997412 |
| disease emergence in multi-host epidemic models. | most pathogens are capable of infecting multiple hosts. these multiple hosts provide many avenues for the disease to emerge. in this investigation, we formulate and analyse multi-host epidemic models and determine conditions under which the disease can emerge. in particular, sis and sir epidemic models are formulated for a pathogen that can infect n different hosts. the basic reproduction number is computed and shown to increase with n, the number of hosts that can be infected. therefore, the po ... | 2007 | 17012365 |
| [acute myopia and progressive acute renal failure in a 28-year old active horsewoman]. | a 28-year old active sportswoman was admitted to hospital suffering from fever, menigeal irritation, acute myopia and progressive acute renal failure. showing signs of polyserositis in combination with pulmonary granulomatous changes a collagenosis as well as an atypical pneumonia was excluded first. due to the renal loss of function a renal biopsy was taken with the typical histological result of a hantavirus infection. this could be confirmed serologically in the following. with symptomatic tr ... | 2006 | 16810539 |
| maternal antibodies postpone hantavirus infection and enhance individual breeding success. | the transfer of maternal antibodies from mother to progeny is a well-known phenomenon in avian and mammalian species. optimally, they protect the newborn against the pathogens in the environment. the effect of maternal antibodies on microparasite transmission dynamics may have important consequences for both the fitness of the host and the epizootic processes of the pathogens. however, there is a scarcity of studies examining these effects in free-living wild species. we studied the influence of ... | 2006 | 17015326 |
| development of novel immunoglobulin g (igg), iga, and igm enzyme immunoassays based on recombinant puumala and dobrava hantavirus nucleocapsid proteins. | human infections with asian and european hantaviruses can result in hemorrhagic fever with renal syndromes of differing severities characterized by renal dysfunction and sometimes by pulmonary symptoms. for the serological detection of human infections by hantaviruses relevant for europe, we developed monoclonal antibody capture immunoglobulin g (igg) and iga enzyme-linked immunosorbent assays (elisas) based on yeast-expressed nucleocapsid proteins of puumala and dobrava hantaviruses. moreover, ... | 2006 | 17021245 |
| mild course of puumala nephropathy in children in an area with sporadic occurrence hantavirus infection. | the first three children with puumala virus nephropathy diagnosis in the czech republic are reported on. a boy and two girls were admitted with symptoms of interstitial nephritis. the medical history in all children revealed flu-like symptoms. all patients were mildly pyrexial and had elevated erythrocytes sedimentation rate, c-reactive protein and low hemoglobin levels. serum creatinine levels were elevated and proteinuria exceeded 700 mg/l in all children. tubular proteinuria, glycosuria, high ... | 2006 | 17024393 |
| dynamics of puumala virus infection in bank voles in ardennes department (france). | the hantaviruses (genus hantavirus, family bunyaviridae) include human pathogens and occur worldwide. in western and central europe, the predominant serotype is puumala (puu) virus, which causes epidemic nephropathy. voles are considered to be the main reservoir and the vector of puu virus. a total of 719 rodents (mainly clethrionomys glareolus, apodemus sp.) trapped by capture-mark-recapture (cmr) in four sites in ardennes department (france) between april 2004 and october 2005 were tested for ... | 2006 | 17027178 |
| sin nombre viral rna load in patients with hantavirus cardiopulmonary syndrome. | to address the role that viral load plays in pathogenesis in patients with hantavirus cardiopulmonary syndrome (hcps), we quantified sin nombre virus s segment viral rna in plasma samples from 27 acutely ill patients. for 6 patients, we examined viral load in matched plasma, urine, and/or tracheal aspirate throughout the time when the patients were in intensive care. peak titers in plasma reached 1.8 x 106 copies/ml; none of the patients had viral rna in urine. titers in tracheal aspirates did n ... | 2006 | 17054070 |
| update on emerging infections from the centers for disease control and prevention. hantavirus pulmonary syndrome--five states, 2006. | | 2006 | 17061318 |
| [epizootology of hemorrhagic fever with renal syndrome in the central chernozem region]. | a total of 5149 small mammals belonging to 16 species were collected from the lipetsk, voronezh, and belgorod regions (40 administrative districts) in 2003-2004 and examined by elisa and ifa to detect hantavirus antigen and antibodies in the lung tissues. hantavirus circulation was revealed in 13 species, the highest hantavirus activity being detected in field (apodemus agrarius) and small wood (a. (s) uralensis) mice (dobrava-belgrad virus), bank (clethrionomis glareolus) (puumala virus) and co ... | 2006 | 17087062 |
| serological survey for viral pathogens in turkish rodents. | wild rodents (n = 330) were trapped around the villages of altindere and coşandere (maçka, trabzon province), ayder, ortan, and yolkiyi (camlihemşin, rize province), and bozdag (odemiş, izmir province) in northeastern and western turkey during april 2004. samples were tested for arenavirus, hantavirus, and cowpox virus (family poxviridae, genus orthopoxvirus, cpxv) antibodies by using immunofluorescence assays (ifas). antibodies against arenaviruses were found in eight of 330 (2.4%) rodents. are ... | 2006 | 17092901 |
| cross-reactive immune responses in mice after genetic vaccination with cdna encoding hantavirus nucleocapsid proteins. | hantaviruses cause hemorrhagic fever with renal syndrome (hfrs) in about 150,000 individuals in eurasia, and several hundred cases of hantavirus pulmonary syndrome (hps) on the american continent annually. there is consequently a need for rapid diagnostics and effective prevention of hantaviral infections. in this study we have performed dna-vaccination of mice with full-length genes encoding the immunogenic nucleocapsid protein (np) of puumala (puuv), seoul (seov) and sin nombre virus (snv). th ... | 2007 | 17110000 |
| geographical distribution of hantaviruses in thailand and potential human health significance of thailand virus. | phylogenetic investigations, sequence comparisons, and antigenic cross-reactivity studies confirmed the classification of thailand virus (thaiv) as a distinct hantavirus species. the examination of sera from 402 rodents trapped in 19 provinces of thailand revealed that five greater bandicoot rats (bandicota indica) and one lesser bandicoot rat (b. savilei) from four provinces were focus reduction neutralization test (frnt) antibody-positive for thaiv. one of 260 patients from surin province in t ... | 2006 | 17124002 |
| catacamas virus, a hantaviral species naturally associated with oryzomys couesi (coues' oryzomys) in honduras. | the primary objective of this study was to extend our knowledge of the geographical distribution, genetic diversity, and natural host associations of the hantaviruses indigenous to north america. antibody to a hantavirus was found in 5 (20.8%) of 24 coues' oryzomys (oryzomys couesi) and none of 41 other rodents captured near the town of catacamas in eastern honduras, and a hantavirus was isolated from one of the antibody-positive coues' oryzomys. analyses of nucleotide and amino acid sequence da ... | 2006 | 17124003 |
| serological evidence for borna disease virus infection in humans, wild rodents and other vertebrates in finland. | borna disease virus (bdv) can infect many vertebrate species, including humans. bdv infection may lead to meningoencephalomyelitis in animals. an association with human neuropsychiatric diseases has been reported, but the causal relationship between bdv and human disease remains unclear. | 2007 | 17129759 |
| rapid immunochromatographic test for hantavirus andes contrasted with capture-igm elisa for detection of andes-specific igm antibodies. | hantavirus is associated with hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). the clinical diagnosis of hantavirus infections has been confirmed routinely by the immunofluorescence antibody assay (ifa) or enzyme-linked immunosorbent assay (elisa). a rapid and easy diagnostic test for hantavirus infection is required. a new immunochromatographic assay for hantavirus, poc-puu, useful for the diagnosis of epidemic nephrophaty associated with hantavirus puumala ... | 2007 | 17133547 |
| implication of phylogenetic systematics of rodent-borne hantaviruses allows understanding of their distribution. | hantaviruses' distribution is reassessed after performing a cladistic analysis on 93 strains isolated from rodents, and one used as outgroup: thottapalayam isolated from a shrew. while most hantaviruses found in wild animals were collected in northern asia, europe, north america, and south america, only thottapalayam and thailand were found in south and southeastern asia. thottapalayam is highly divergent from the other known hantaviruses and may represent the emerging tip of a different lineage ... | 2006 | 17135493 |
| [emergence of new pneumonia: besides severe acute respiratory syndrome]. | important epidemiological modifications have been registered in respiratory infections, both in immunocompetent and immunocompromised hosts. pathogens with modified antibiotic susceptibility patterns have emerged, which display an increased antibiotic resistance, such as s. pneumoniae, s. aureus, h. influenzae. this trait has a strong impact on the therapeutic choices, particularly when an empiric antibiotic treatment is selected. the prevalence of bacterial species showing non-susceptibility to ... | 2006 | 17146421 |
| profiling helper t cell subset gene expression in deer mice. | deer mice (peromyscus maniculatus) are the most common mammals in north america and are reservoirs for several zoonotic agents, including sin nombre virus (snv), the principal etiologic agent of hantavirus cardiopulmonary syndrome (hcps) in north america. unlike human hcps patients, snv-infected deer mice show no overt pathological symptoms, despite the presence of virus in the lungs. a neutralizing igg antibody response occurs, but the virus establishes a persistent infection. limitations of de ... | 2006 | 16916450 |
| evaluation of promed-mail as an electronic early warning system for emerging animal diseases: 1996 to 2004. | to identify emerging animal and zoonotic diseases and associated geographic distribution, disease agents, animal hosts, and seasonality of reporting in the program for monitoring emerging diseases (promed)-mail electronic early warning system. | 2006 | 17014355 |
| andes hantavirus as possible cause of disease in travellers to south america. | hantaviruses in europe and asia cause haemorrhagic fever with renal syndrome and epidemic nephritis (mortality rate <1-15%). new strains of hantaviruses cause hantavirus pulmonary syndrome (hps) from canada to south america. andes virus mortality rate is about 30% in chile. | 2007 | 17161316 |
| phylogenetic and geographical relationships of hantavirus strains in eastern and western paraguay. | recently, we reported the discovery of several potential rodent reservoirs of hantaviruses in western (holochilus chacarius) and eastern paraguay (akodon montensis, oligoryzomys chacoensis, and o. nigripes). comparisons of the hantavirus s- and m-segments amplified from these four rodents revealed significant differences from each another and from other south american hantaviruses. the alp strain from the semiarid chaco ecoregion clustered with leguna negra and rio mamore (ln/rm), whereas the bm ... | 2006 | 17172380 |
| use of igg avidity to indirectly monitor epizootic transmission of sin nombre virus in deer mice (peromyscus maniculatus). | an igg avidity assay was developed to differentiate deer mice that had recently acquired sin nombre virus (snv) from those that were infected in the distant past. using this procedure, low avidity antibodies were predominantly detected in experimentally infected deer mice (89.5%) within the first 30 days post-inoculation. the assay was then applied to sera from naturally infected deer mice collected during a field investigation associated with a cluster of hantavirus pulmonary syndrome cases. a ... | 2006 | 17172381 |
| radiology of biological weapons--old and the new? | | 2007 | 17174174 |
| [hantavirus in zulia state?]. | during the last years, there has been a worldwide rise in the number of infectious diseases caused by newly discovered agents, in some cases and in others, by variants known as emergent or reemergent pathogens. the analysis of the nucleotide sequences of four rna-positive hantaviruses (s. altoni) suggests that the caño delgadito virus is widely distributed in the venezuelan plains. a similar distribution has been found for some isolates and strains of a novel hantavirus (proposed name "mamporal" ... | 2006 | 17176900 |
| development of serological assays for thottapalayam virus, an insectivore-borne hantavirus. | thottapalayam virus (tpmv), a member of the genus hantavirus in the family bunyaviridae, was isolated from an insectivore, suncus murinus (musk shrew), captured in southern india in 1964. while the isolation of tpmv predates the discovery of the prototype hantaan virus, little is known about its genetics and biology. to date, preliminary evidence suggests that tpmv differs significantly, both antigenically and genetically, from all known rodent-borne hantaviruses. however, since detailed epizoot ... | 2007 | 17182762 |
| tick-borne encephalitis virus in clethrionomys glareolus in the czech republic. | a total of 474 specimens from 157 rodents caught at the military training area of boletice in the south of the czech republic from may to november 1999 were screened for tbev by nested pcr. tbev-specific rna was amplified from lung, kidney, and spleen derived from one clethrionomys glareolus in the first rt-pcr round. sequence analysis revealed a 100% identity to the tbev strain neudoerfl. tbev presence in the sample was confirmed by mouse brain passage of backup samples and cell culture. the re ... | 2006 | 17187572 |
| evaluation of the efficacy of disinfectants against puumala hantavirus by real-time rt-pcr. | puumala virus, a hantavirus belonging to the bunyaviridae family, causes a human disease known as nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome. the implementation of effective decontamination procedures is critical in hantavirus research to minimize the risk of personnel exposure. this study investigated the efficacy of clidox((r)), dettol((r)), ethanol, halamid-d((r)), peracetic acid, sodium hypochloride and virkon((r))s for inactivating puumala virus. a real-tim ... | 2007 | 17188760 |
| developing global climate anomalies suggest potential disease risks for 2006-2007. | el niño/southern oscillation (enso) related climate anomalies have been shown to have an impact on infectious disease outbreaks. the climate prediction center of the national oceanic and atmospheric administration (noaa/cpc) has recently issued an unscheduled el niño advisory, indicating that warmer than normal sea surface temperatures across the equatorial eastern pacific may have pronounced impacts on global tropical precipitation patterns extending into the northern hemisphere particularly ov ... | 2006 | 17194307 |
| andes and prospect hill hantaviruses differ in early induction of interferon although both can downregulate interferon signaling. | hantavirus pulmonary syndrome (hps) is a severe respiratory disease which is thought to result from a dysregulated immune response to infection with pathogenic hantaviruses, such as sin nombre virus or andes virus (andv). other new world hantaviruses, such as prospect hill virus (phv), have not been associated with human disease. activation of an antiviral state and cell signaling in response to hantavirus infection were examined using human primary lung endothelial cells, the main target cell i ... | 2007 | 17202220 |
| hantaan virus infection among human and rat populations in alexandria. | hantaan virus has recently been identified as the cause of the rodent borne viral zoonosis collectively termed hantavirus disease (hvd). although the disease is mainly endemic in two main areas, clinical forms as well as positive antibody testing from healthy persons throughout the world have been reported denoting a worldwide presence. rattus species are almost universally implicated as hantavirus reservoirs. screening for hantavirus antibodies was done on 637 samples of human sera and 861 rode ... | 1996 | 17217009 |
| the coiled-coil domain structure of the sin nombre virus nucleocapsid protein. | hantaviruses can cause hemorrhagic fever with a renal syndrome and hantavirus pulmonary syndrome when transmitted to humans. the nucleocapsid protein of hantaviruses encapsidates viral genomic rna and associates with transcription and replication complexes. both the amino and carboxy termini of the nucleocapsid protein had been predicted to form trimers prior to the formation of the ribonucleoprotein. crystal structures of amino-terminal fragments of the nucleocapsid protein showed the formation ... | 2007 | 17222867 |
| computer-aided diagnosis with potential application to rapid detection of disease outbreaks. | our objectives are to quickly interpret symptoms of emergency patients to identify likely syndromes and to improve population-wide disease outbreak detection. we constructed a database of 248 syndromes, each syndrome having an estimated probability of producing any of 85 symptoms, with some two-way, three-way, and five-way probabilities reflecting correlations among symptoms. using these multi-way probabilities in conjunction with an iterative proportional fitting algorithm allows estimation of ... | 2007 | 17225213 |
| utilization of hantavirus antibody results generated over a five-year period to develop an improved serologic algorithm for detecting acute sin nombre hantavirus infection. | in the united states most cases of hantavirus pulmonary syndrome (hps) are caused by the sin nombre virus (snv) and are typically identified by serology. the goal of this study was to assess the performance of our hantavirus serologic testing algorithm by reviewing results generated over five years. sera were screened for pan-hantavirus immunoglobulin (ig)g and igm by enzyme-linked immunosorbent assay (elisa). screen igg+ sera were then tested by immunoblot for snv glycoprotein-specific igg, and ... | 2007 | 17245757 |
| ribavirin, human convalescent plasma and anti-beta3 integrin antibody inhibit infection by sin nombre virus in the deer mouse model. | the new world hantavirus sin nombre virus (snv) is an aetiological agent for the often-fatal hantavirus cardiopulmonary syndrome (hcps). there is no disease model for snv and specific treatments for hcps do not exist. by using the deer mouse infectious model, the in vivo inhibitory potential of ribavirin, human anti-snv immune plasma (hip), an anti-beta3 antibody (reopro) and a polyclonal rabbit anti-recombinant nucleocapsid (n) antibody against snv was investigated. concurrent intraperitoneal a ... | 2007 | 17251568 |
| deer mouse movements in peridomestic and sylvan settings in relation to sin nombre virus antibody prevalence. | prevalence of antibody to sin nombre virus (snv) has been found to be nearly twice as high in deer mice (peromyscus maniculatus) in peridomestic settings as in sylvan settings in two studies in montana and one in new mexico. we investigated whether this difference may be related to a difference in deer mouse movements in the two settings. we used radiotelemetry to determine home range size and length of movement for 22 sylvan (1991-1992) and 40 peridomestic deer mice (1995-1999). we also determi ... | 2006 | 17255448 |
| changes in sin nombre virus antibody prevalence in deer mice across seasons: the interaction between habitat, sex, and infection in deer mice. | we examined the impact of season and habitat on sin nombre virus (snv) seroprevalence in deer mice (peromyscus maniculatus) in utah's great basin desert from may 2002 through summer 2003. low mouse captures in 2002 limited analysis for that year. in two seasons during 2003, mouse density and sagebrush cover were positively linked (spring: r = 0.8, p = 0.01; summer: r = 0.8, p = 0.04). in the spring, seroprevalence was negatively correlated with density (r = -0.9, p< 0.01); male and female antibo ... | 2006 | 17255449 |
| morphological characterization of hantavirus hv114 by electron microscopy. | this study was sought to investigate the propagation and morphogenesis of a new strain of hantavirus, hv114. | 2007 | 17259735 |
| the expression and genetic immunization of chimeric fragment of hantaan virus m and s segments. | hemorrhagic fever with renal syndrome (hfrs), which is characterized by severe symptoms and high mortality, is caused by hantavirus. there are still no effective prophylactic vaccines directed to hfrs until now. in this research, we fused expressed g2 fragment of m segment and 0.7kb fragment of s segment. we expect it could be a candidate vaccine. chimeric gene g2s0.7 was first expressed in prokaryotic expression system pgex-4t. after inducing expressed fusion proteins, gst-g2s0.7 was induced an ... | 2007 | 17266922 |
| hantavirus pulmonary syndrome: high-resolution ct findings in one patient. | physical examination demonstrated petechiae, leg oedema and mild dyspnoea. chest radiograph showed minimal bilateral hazy increased opacification, mainly on the right side, and small bilateral pleural effusions. high-resolution ct demonstrated extensive bilateral ground-glass opacities most severe in the middle and lower lung zones. also noted were a few slightly thickened interlobular septa, a few poorly defined small nodules, bronchial wall thickening and small bilateral pleural effusions. blo ... | 2007 | 17267465 |
| degrons at the c terminus of the pathogenic but not the nonpathogenic hantavirus g1 tail direct proteasomal degradation. | pathogenic hantaviruses cause two human diseases: hantavirus pulmonary syndrome (hps) and hemorrhagic fever with renal syndrome (hfrs). the hantavirus g1 protein contains a long, 142-amino-acid cytoplasmic tail, which in ny-1 virus (ny-1v) is ubiquitinated and proteasomally degraded (e. geimonen, i. fernandez, i. n. gavrilovskaya, and e. r. mackow, j. virol. 77: 10760-10768, 2003). here we report that the g1 cytoplasmic tails of pathogenic andes (hps) and hantaan (hfrs) viruses are also degraded ... | 2007 | 17267477 |
| seasonal variation of north american form of gigantolaelaps mattogrossensis (acari: laelapidae) on marsh rice rat in southern coastal texas. | the ectoparasites of a small mammal community within an intertidal zone in the upper gulf coast region of texas were studied to assess the seasonal variation in abundances of the mite gigantolaelaps mattogrossensis (fonseca) (acari: laelapidae) on the marsh rice rat, oryzomys palustris (harlan). further study into the ecology and dynamics of this parasite-host relationship was deemed to be necessary to expand the understanding of these potential participants in the ecology of bayou hantavirus, a ... | 2007 | 17294924 |
| cross-sectional survey on hantavirus seroprevalence in canton st. gallen, switzerland. | in 2002 the first endemic hantavirus infection in switzerland was detected only by chance following a broad spectrum of diagnostics. this raised the question, whether hantavirus infection should be included in the differential diagnosis of febrile illness of patients in switzerland. in order to estimate the frequency of hantavirus infections in switzerland, this survey on hantaviral seroprevalence was conducted in the canton st. gallen. | 2007 | 17299664 |
| [progress on the study of biological characteristic of hantavirus]. | | 2006 | 17299972 |
| detection of circulating antigen with a mab-based sandwich-elisa and its comparison with specific igm detection in sera of patients with hemorrhagic fever with renal syndrome. | a monoclonal antibody (1h2) against nucleocapsid protein of hantavirus was developed. a sandwich enzyme-linked immunosorbent assay (elisa) with the monoclonal antibody (mab) was established and evaluated for detecting circulating antigen (cag) in serum of patients with hemorrhagic fever with renal syndrome (hfrs). results were compared to that of an immunoglobulin m (igm)-detecting elisa. of 143 patients with hfrs, 106 were positive for cag of hantavirus and 128 positive for specific igm. among ... | 2007 | 17316085 |
| selection and characterization of scfv antibodies against the sin nombre hantavirus nucleocapsid protein. | rodent-borne hantaviruses cause hemorrhagic fever with renal syndrome (hfrs) in the old world and hantavirus cardio-pulmonary syndrome (hcps) in the new. most cases of hcps in north america are caused by sin nombre virus (snv). current viral detection technologies depend upon the identification of anti-viral antibodies in patient serum. detection of viral antigen may facilitate earlier detection of the pathogen. we describe here the characterization of two single-chain fv antibodies (scfvs), sel ... | 2007 | 17336997 |
| ex vivo stability of the rodent-borne hantaan virus in comparison to that of arthropod-borne members of the bunyaviridae family. | the possible effect of virus adaptation to different transmission routes on virus stability in the environment is not well known. in this study we have compared the stabilities of three viruses within the bunyaviridae family: the rodent-borne hantavirus hantaan virus (htnv), the sand fly-borne phlebovirus sandfly fever sicilian virus (sfsv), and the tick-borne nairovirus crimean-congo hemorrhagic fever virus (cchfv). these viruses differ in their transmission routes: sfsv and cchfv are vector bo ... | 2007 | 17337567 |
| [the new-world hantaviruses. ecology and epidemiology of an emerging virus in latin america]. | the hantaviruses are a group of emerging rodent-borne pathogens (family bunyaviridae; genus hantavirus) that are etiologic agents for hemorrhagic fever with renal syndrome (hfrs) in europe and asia and hantavirus cardiopulmonary syndrome (hcps) in the americas. hfrs is associated with rodents of the family muridae, subfamilies murinae and arvicolinae; hps is associated with rodents of the subfamily sigmodontinae. since the identification of hcps in usa in 1993, a large number of cases of hps and ... | 2006 | 16977974 |
| [isolation and characterization of hantavirus carried by rodents in huludao, liaoning province]. | to investigate the hantavirus infection and their genotype in rodents in huludao. | 2006 | 17152513 |
| demographic factors associated with prevalence of antibody to sin nombre virus in deer mice in the western united states. | we used long-term data collected for up to 10 yr (1994-2004) at 23 trapping arrays (i.e., webs and grids) in arizona, colorado, montana, and new mexico to examine demographic factors known or suspected to be associated with risk of infection with sin nombre virus (snv) in its natural host, the deer mouse (peromyscus maniculatus). gender, age (mass), wounds or scars, season, and local relative population densities were statistically associated with the period prevalence of antibody (used as a mar ... | 2007 | 17347388 |