Publications

community structure and prevalence of hantavirus infection in rodents: a geographic division of the enzootic area in far eastern russia.	the results of an extensive rodent trapping effort throughout the southern part of far eastern russia and hantavirus antigen screening of tissues were used to develop a multifaceted approach for the geographic division of the enzootic territory of hantavirus. four species of rodents (apodemus agrarius, apodemus peninsulae, microtus fortis, and clethrionomys rufocanus) comprised 88.5 percent of 10,595 captured rodents and 94.1 percent of 996 antigen-positive animals. rodent fauna and the prevalen ...	1997	9221739
[the hantavirus pulmonary syndrome (its etiology, epidemiology, pathogenesis, clinical manifestations and the diagnosis of a new infectious disease)].		1997	9229840
hantavirus antibodies in new york.		1997	9230017
hantavirus pulmonary syndrome.	the objective of this report is to summarize the medical literature relevant to the epidemiology, diagnosis, treatment, and control of hantavirus pulmonary syndrome (hps). the english language literature was searched from january 1993 through march 1995, manually and using medline, including conference proceedings and reference lists. we selected relevant articles on clinical aspects of hps. hps is a newly described illness that progresses through three phases: prodromal, cardiopulmonary, and co ...	1997	9097374
common questions about hantavirus pulmonary syndrome.		1997	9103926
hantavirus pulmonary syndrome in the rural area of juquitiba, são paulo metropolitan area, brazil.		1997	9640790
hantavirus pulmonary syndrome in the province of rio negro, argentina, 1993-1996.	early in 1995 the first case of hantavirus pulmonary syndrome was serologically confirmed in el bolsón (province of río negro, argentina), corresponding to the third outbreak reported in argentina. a total of 26 cases of hps related to the andean region of rio negro province, were reported from 1993 to 1996, 17 in el bolsón, 4 in san carlos de bariloche, and 5 in buenos aires. the incidence rate was 5.03 x 100,000 with a mortality rate of 51.85 x 100. the occurrence of cases was mainly seasonal, ...	1997	9640780
[the pulmonary syndrome produced by hantaviruses].		1997	9654969
emerging pathogens: the hong kong experience.		1997	9594216
hantavirus pulmonary syndrome. report of the first three cases in são paulo, brazil.	the hantavirus pulmonary syndrome was first recognized in cases that occurred in the u.s. in 1993, which served as an alert not only for american physicians but also for physicians in other countries for the identification of the disease. in the city of são paulo, brazil, 3 cases of the syndrome were recorded in 1993. the patients were young brothers residing in the mata atlântica (atlantic forest) region submitted to recent deforestation. two of the patients died of acute respiratory insufficie ...	1997	9640788
serological diagnosis of hantavirus infections by an enzyme-linked immunosorbent assay based on detection of immunoglobulin g and m responses to recombinant nucleocapsid proteins of five viral serotypes.	worldwide, hantaviruses cause more than 100,000 human infections annually. rapid and accurate methods are important both in monitoring acute infections and for epidemiological studies. we and others have shown that the amino termini of hantavirus nucleocapsid proteins (ns) are sensitive tools for the detection of specific antibodies in hantavirus disease. accordingly, we expressed truncated ns (amino acids 1 to 117) in escherichia coli from the five hantaviruses known to be pathogenic to man; ha ...	1997	9114393
genetic characterization and phylogeny of andes virus and variants from argentina and chile.	andes virus, one of five hantaviruses known to cause hantavirus pulmonary syndrome (hps), emerged in 1995 in southwestern argentina (lópez et al. (1996) virology 220, 223-226). the complete nucleotide sequence of andes virus s genome segment was determined and compared with sequences of viral rnas in autopsy tissues of more recently reported hps cases from southwestern argentina and south of chile (cases esq h-1/96 and ch h-1/96). andes virus s segment was found to be 1876 nucleotides in length ...	1997	9255937
[epidemic hantavirus nephropathy].		1997	9381000
glomerular basement membrane antibodies in hantavirus disease (hemorrhagic fever with renal syndrome).	the prevalence of antibodies against glomerular basement membrane (gbm) antigens in sera from 47 patients with serologically verified hantavirus (puumala serotype) infection were investigated. antibodies were measured by immunoassays using as antigen a crude human gbm preparation and the nc1 portion of type iv collagen (goodpasture antigen), respectively. seventy-seven percent of the patients had igm antibodies against the non-goodpasture glomerular basement membrane (non-gp gbm) in their acute ...	1997	9342483
sequence analysis of the puumala hantavirus sotkamo strain l segment.	sequence of the puumala virus (puu) sotkamo strain l segment is provided, completing the total genome of this prototype puu virus strain. the l segment is 6530 nucleotides long and it can encode 2156 amino-acids-long l protein, rna-dependent rna polymerase. the strain sotkamo, originally isolated in finland, showed for the l genome segment nucleotide (84.6%) and amino acid (97.3%) homology to a previously sequenced puu russian isolate, strain bashkiria/cg1820 (b1820) and the l genome segment app ...	1997	9381791
evaluation of serological methods for diagnosis of puumala hantavirus infection (nephropathia epidemica).	nephropathia epidemica (ne), puumala (puu) virus infection, is a febrile disease which is commonly associated with acute renal impairment. to differentiate ne from other acute febrile illnesses, a rapid and reliable serological diagnosis is important, and a number of different protocols have recently been introduced. in the present report we describe a comparative evaluation of six puu virus immunoglobulin m (igm) and seven igg enzyme-linked immunosorbent assay (elisa) protocols based on native, ...	1997	9399531
hantavirus pulmonary syndrome: cd8+ and cd4+ cytotoxic t lymphocytes to epitopes on sin nombre virus nucleocapsid protein isolated during acute illness.	in 1993 a number of cases of unexplained adult respiratory syndrome occurred in the southwestern united states. the illness was characterized by a prodrome of fever, myalgia, and other symptoms followed by the rapid onset of a capillary leak syndrome with hemoconcentration, thrombocytopenia, and pulmonary edema. viral rna sequences in the lungs identified a new member of the hantavirus genus, sin nombre virus (snv), unique to north america. pulmonary endothelial cells were heavily infected but w ...	1997	9400611
impaired pulmonary function in patients with hemorrhagic fever with renal syndrome.	pulmonary and cardiac functions were investigated in 13 patients hospitalized with nephropathia epidemica, a european form of hemorrhagic fever with renal syndrome. as compared with reference values, the patients' diffusion capacity for carbon monoxide was decreased (p = .002) and pulmonary clearance of inhaled technetium-99m-labeled diethylenetriamine pentaacetic acid was increased (p = .002). in four of 11 patients, arterial blood gas analysis disclosed a reduction in partial pressure of o2 (< ...	1997	9402362
the enigmatic haemorrhagic fevers.		1997	9496275
oligoryzomys flavescens (rodentia, muridae): gene flow among populations from central-eastern argentina.	in species acting as hosts of infectious agents, the extent of gene flow between populations is of particular interest because the expansion of different infectious diseases is usually related to the dispersal of the host. we have estimated levels of gene flow among populations of the sigmodontine rodent oligoryzomys flavescens, in which high titers of antibodies have been detected for a hantavirus in argentina that produces a severe pulmonary syndrome. enzyme polymorphism was studied by means o ...	1997	9465403
pleural fluid characteristics in hantavirus pulmonary syndrome.	hantavirus pulmonary syndrome (hps), is a rodent-borne, acute, often fulminant cardiorespiratory illness. noncardiogenic pulmonary edema is prominent in hps as is cardiac dysfunction. pleural effusions are commonly noted in patients with hps and have been thought to be exudative. this report describes the prevalence and characteristics of pleural effusions by an assessment of chest radiographs for the presence of pleural fluid and reviews all pleural fluid specimens obtained from patients with h ...	1997	9377934
baculovirus expression of a human g2-specific, neutralizing igg monoclonal antibody to puumala virus.	we amplified by polymerase chain reaction the heavy- and light-chain antibody genes of a human hybridoma secreting a neutralizing, igg monoclonal antibody to the g2 protein of puumala virus. the heavy- and light-chain genes were cloned and sequenced and the deduced amino acids were aligned with those of other human antibodies to identify the constant and variable regions. the genes were cloned into the baculovirus plasmid transfer vector pacuw51 such that the heavy-chain and light-chain genes we ...	1997	9281505
[viral haemorrhagic fever].	viral haemorrhagic fever denotes various kinds of febrile illness caused by certain viruses which often presents with bleeding tendency and occasionally shock. out of these, the four maladies, lassa fever, ebola haemorrhagic fever, marburg haemorrhagic fever and crimean-congo haemorrhagic fever which are endemically present in africa or eastern europe, are known to be such diseases with high man-to-man communicability. these four haemorrhagic fevers are, therefore, designated as special conditio ...	1997	9283226
a small-scale survey of hantavirus in mammals from indiana.	in order to determine if hantaviruses were present in mice and other small mammals in indiana (usa), small mammals were trapped in brown, laporte, tippecanoe and whitley counties. sixty-seven small mammals were trapped during august and september 1994. sixty-three peromyscus leucopus, one microtus pennsylvanicus, one zapus hudsonius and two blarina brevicauda were captured and tested for hantaviruses. six p. leucopus were found to have antibody to sin nombre virus (sn) by igg elisa, and a 139 bp ...	1997	9391967
epitope mapping by phage display: random versus gene-fragment libraries.	we present a comparative study on epitope mapping of four monoclonal antibodies directed against four different antigens using alternative phage display techniques and peptide scanning: mab215 reacts with the largest subunit of rna polymerase ii, mabbp53-11 with the tumor suppressor protein p53, mabgdo5 with the hantaan virus glycoprotein g2 and mabl13f3 with the hantaan virus nucleocapsid protein. epitopes were determined (i) by gene-fragment phage display libraries, constructed by dnasei diges ...	1997	9328567
the first human isolate of puumala virus in scandinavia as cultured from phytohemagglutinin stimulated leucocytes.	a virus isolate was recovered from blood leucocytes of a patient with nephropathia epidemica (ne). leucocytes were isolated from edta-blood by dextran sedimentation and cultured on monolayers of vero e6 cells in the presence of phytohemagglutinin (pha) in roller tubes during the first 72 hours of incubation followed by rolling culture for three weeks in total. thereafter the first subculture was done in a plastic flask and afterward at at least 6 week intervals. antigen was first detected after ...	1997	9334926
puumala virus and two genetic variants of tula virus are present in austrian rodents.	puumala and tula viruses are hantaviruses found in europe and are associated with the rodents clethrionomys glareolus and microtus arvalis, respectively. puumala virus is associated with the human disease nephropathia epidemica. in austria, ten clinically diagnosed cases of nephropathia epidemica, presumably caused by puumala virus infection, have been reported but not virologically confirmed [leschinskaya et al., 1991; aberle et al., 1996]. to identify the hantaviruses that are present in austr ...	1997	9334930
effectiveness of recorded messages to communicate the risk of acquiring hantavirus pulmonary syndrome.	to determine the effectiveness of a recorded information line in communicating health risk during the emergence of a new disease, hantavirus pulmonary syndrome (hps), in the edmonton area and to study the accuracy of recall of information about hantavirus among the general public.	1997	9336097
hantavirus pulmonary syndrome in the americas.		1997	9341305
[emerging infectious diseases--hantavirus infections].		1997	9480304
human-to-human transmission of hantaviruses.		1997	9284808
[approaches to selecting a strain of hantavirus for developing an inactivated brain vaccine].	four strains isolated from patients with hemorrhagic fever with the renal syndrome and from field mice, natural hosts of hantavirus, were examined in order to choose a functionally active strain fit for the development of inactivated hantavirus brain vaccine. virulent strain pm-10508/89 isolated from mouse lungs was chosen. the strain was highly virulent for newborn mice and rats: 100% of infected mice aged up to 24 h and 75% of rats developed an acute lethal infection upon challenge. the incuba ...	1997	9297337
dobrava hantavirus outbreak in russia.		1997	9298001
an outbreak of hantavirus pulmonary syndrome in western paraguay.	during an investigation of hantavirus pulmonary syndrome (hps) in paraguay in 1995, sera from persons with hps-like illness, houshold contacts of confirmed hps case-patients, and a sample of the area residents were analyzed by elisa for antibodies to sin nombre virus (snv). rodent serosurveys and analysis of precipitation records were also conducted. twenty-three of 24 available probable cases were snv antibody-positive, 17 of whom were ill between july 1995 and january 1996. four (14.8%) of 27 ...	1997	9311636
a colorimetric pcr-enzyme immunoassay to identify hantaviruses.	hantaviruses cause two serious human diseases: hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome. at least nine hantaviruses are known to be pathogenic for humans and numerous others, with unknown disease potential, have been detected in rodents. assays to quickly identify specific hantaviruses would be useful both for clinical diagnosis and in risk assessment studies.	1997	9316733
puumala and dobrava viruses cause hemorrhagic fever with renal syndrome in bosnia-herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera.	hantavirus infection was diagnosed serologically by mu-capture igm and igg elisas in hemorrhagic fever with renal syndrome (hfrs) patients admitted to tuzla hospital, bosnia-herzegovina. the results indicated that more than one hantavirus caused the outbreak. to address the question of which hantavirus serotypes were involved, sequentially drawn sera were analyzed by focus reduction neutralization test (frnt) for antibodies against puumala, hantaan, dobrava, and seoul hantaviruses. the data reve ...	1997	9298732
the investigation of emerging and re-emerging viral diseases: a paradign.	emerging virus infections are defined as previously nonthreatening viruses that can decimate new populations by finding fresh hosts and vectors--often with the help of humans who introduce new species into virgen environment, several etiologic agents of these diseases, some of the interacting factors that contribute to their development and the role of molecular medicine in their understanding is discussed.	1997	9419931
cell culture adaptation of puumala hantavirus changes the infectivity for its natural reservoir, clethrionomys glareolus, and leads to accumulation of mutants with altered genomic rna s segment.	this paper reports the establishment of a model for hantavirus host adaptation. wild-type (wt) (bank vole-passaged) and vero e6 cell-cultured variants of puumala virus strain kazan were analyzed for their virologic and genetic properties. the wt variant was well adapted for reproduction in bank voles but not in cell culture, while the vero e6 strains replicated to much higher efficiency in cell culture but did not reproducibly infect bank voles. comparison of the consensus sequences of the respe ...	1997	9371614
nephropathia epidemica infection during first trimester of pregnancy--normal fetal outcome.		1997	9351417
papillary necrosis: a late complication of nephropathia epidemica?	in the following we describe a case of nephropathia epidemica, which exhibited, a few years after acute infection, arterial hypertension and multiple papillary necrosis. papillary necrosis was diagnosed by i.v. pyelography and ct scan. a complete evaluation of the patient failed to show any other etiology for medullary necrosis. if arterial hypertension has already been reported as a complication of nephropathia epidemica, papillary necrosis is exceptional, but may be explained by the severe vas ...	1997	9352163
prevalence of serum antibodies to hantaviruses in northern sweden as measured by recombinant nucleocapsid proteins.	an enzyme-linked immunosorbent assay (elisa) based on recombinant nucleocapsid protein (rn delta) (aa 1-117) of hantaan, seoul, dobrava, sin nombre and puumala hantaviruses was used to determine the prevalence of antibodies among randomized and stratified individuals from northern sweden. in total, 137/1533 individuals (8.9%) had specific serum igg antibodies to puumala virus, the only hantavirus known to occur in the region. the prevalence of antibodies to puumala virus (8.9%) was determined to ...	1997	9360248
hantavirus infection in scid mice.	severe combined immunodeficiency (scid) mice were inoculated with hantaan virus strain 76-118 (htn) or seoul virus strain sr-11 (sr) of hantaviruses. susceptibility of scid mice was compared with those of immunocompetent adult mice, newborn mice and nude mice. scid mice inoculated with htn or sr died 32 to 35 days after infection. unlike newborn mice which also died of hantavirus infection, scid mice survived longer than newborn mice and showed typical wasting symptoms rather than nervous sympto ...	1997	9362032
laguna negra virus associated with hps in western paraguay and bolivia.	a large outbreak of hantavirus pulmonary syndrome (hps) recently occurred in the chaco region of paraguay. using pcr approaches, partial virus genome sequences were obtained from 5 human sera, and spleens from 5 calomys laucha rodents from the outbreak area. genetic analysis revealed a newly discovered hantavirus, laguna negra (ln) virus, to be associated with the hps outbreak and established a direct genetic link between the virus detected in the hps cases and in the c. laucha rodents, implicat ...	1997	9375015
puumala type hantavirus infection with early choleriform syndrome and severe multiorgan failure.		1997	9255656
the english sweating sickness, 1485 to 1551.		1997	9023099
hantavirus infections.		1997	9003738
atypical respiratory pathogens.	the main atypical pathogens in respiratory tract infections are classified on the basis of their ability to cause atypical pneumonia. this is not a well-defined clinical entity, and it is evident that atypical pathogens can sometimes cause 'typical' pneumonias and vice versa. this emphasizes the need for microbiological diagnosis, since it affects the selection of proper treatment, in which beta-lactam antibiotics and aminoglycosides are not effective. moreover, mixed infections caused by atypic ...	1997	11864199
[infections always present at the dawn of the 21st century].	despite the many scientific achievements realized in recent years, infectious diseases still remain the main cause of premature mortality worldwide. it is also growing increasingly difficult to fight infections. tuberculosis and malaria are making a comeback, cholera and yellow fever are making new inroads into countries where they¿ve never been before, other diseases are developing resistance to antibiotics and have in some cases become incurable, and new diseases such as ebola-induced hemorrh ...	1997	12322621
[studies on the experimental transmission of rattus-borne hantavirus by ornithonyssus bacoti].	to demonstrate the role of rat mite (ornithonyssus bacoti) in the transmission of rattus-borne hemorrhagic fever with renal syndrome (hfrs).	1998	12078291
anne boleyn, the sweating sickness, and the hantavirus: a review of an old disease with a modern interpretation.		1998	11619876
implications of the analogy between recombinant cytokine toxicities and manifestations of hantavirus infections.	the etiologic hantavirus of the 1993 emergence of an acute pulmonary failure syndrome in the area around northwestern new mexico was quickly recognized as related to the hantaan virus responsible for the outbreak of korean epidemic hemorrhagic fever (ehf) among un troops in 1951. discovery of the new disease which was named the hantavirus pulmonary syndrome (hps) and its causative agent the sine nombre virus (snv) inspired detailed comparisons between the two disorders. major damage to the epith ...	1998	10850356
emerging infectious diseases and travel medicine.	international movement of individuals, populations, and products is one of the major factors associated with the emergence and reemergence of infectious diseases as the pace of global travel and commerce increases rapidly. travel can be associated with disease emergence because (1) the disease arises in an area of heavy tourism, (2) tourists may be at heightened risk because of their activities, or (3) because they can act as vectors to transport the agent to new areas. examples of recently reco ...	1998	9494841
serologic survey for hantavirus infection in domestic animals and coyotes from new mexico and northeastern arizona.	to determine whether animals had serologic evidence of infection with sin nombre virus (snv).	1998	9540866
hantavirus infection in children in argentina.	clinical hantavirus infection was diagnosed in five argentine children ages 5 to 11 years by immunoglobulin m (igm)- capture enzyme-linked immunosorbent assay using sin nombre virus (snv) antigens. death in three of the children was associated with absence of detectable igg to snv antigens. an additional two cases in healthy children were studied: one, a breast-fed 15-month-old whose mother died of suspected hantavirus pulmonary syndrome (hps) 8 months previously, had hantavirus igg (> 1:6400); ...	1998	9454562
successful treatment of adults with severe hantavirus pulmonary syndrome with extracorporeal membrane oxygenation.	to describe our experience with the use of extracorporeal membrane oxygenation (ecmo) as a rescue therapy in adult patients with severe cardiopulmonary failure from hantavirus pulmonary syndrome.	1998	9468181
small mammal survival and trapability in mark-recapture monitoring programs for hantavirus.	following the 1993 hantavirus pulmonary syndrome (hps) epidemic in the south-western united states, mammalogists and epidemiologists instituted long-term studies to monitor population density and prevalence of infection in rodents which constitute the reservoir for sin nombre virus (snv). in this study, field techniques used in sampling small mammals for snv infection were evaluated to determine if trapping and handling protocols were having significant effects on future trapability or mortality ...	1998	9476220
surveillance of anti-hantavirus antibodies among certain high-risk groups in taiwan.	hemorrhagic fever with renal syndrome is a serious health concern in neighboring countries of taiwan, such as mainland china and korea. in taiwan, only two suspected cases were recorded before 1994. the first confirmed case was reported in 1995, but this proved to be imported. to study hantavirus infection in taiwan, we tested blood collected from garbage collectors, animal handlers, patients with febrile illness of unknown origin, and field rats, the host of hantavirus, for the presence of anti ...	1998	9481070
a survey of hantavirus antibody in small-mammal populations in selected united states national parks.	hantavirus activity in 39 national parks in the eastern and central united states was surveyed by testing 1,815 small mammals of 38 species for antibody reactive to sin nombre virus. antibody-positive rodents were found throughout the area sampled, and in most biotic communities. antibody was detected in 7% of 647 deer mice (peromyscus maniculatus), 2% of 590 white-footed mice (p. leucopus), 17% of 12 rice rats (oryzomys palustris), 3% of 31 cotton rats (sigmodon hispidus), and 33% of 18 western ...	1998	9574803
hantavirus pulmonary syndrome in a chilean patient with recent travel in bolivia.	a case of hantavirus pulmonary syndrome (hps) was serologically confirmed in a critically ill patient in santiago, chile. the patient's clinical course had many similarities to that of other hps patients in north and south america but was complicated by acute severe renal failure. the patient's history included self-reported urban and probable rural rodent exposure during travel in bolivia. comparison of a viral sequence from an acute-phase serum sample with other known hantaviruses showed that ...	1998	9452401
bayou virus-associated hantavirus pulmonary syndrome in eastern texas: identification of the rice rat, oryzomys palustris, as reservoir host.	we describe the third known case of hantavirus pulmonary syndrome (hps) due to bayou virus, from jefferson county, texas. by using molecular epidemiologic methods, we show that rice rats (oryzomys palustris) are frequently infected with bayou virus and that viral rna sequences from hps patients are similar to those from nearby rice rats. bayou virus is associated with o. palustris; this rodent appears to be its predominant reservoir host.	1998	9452404
genetic reassortment among viruses causing hantavirus pulmonary syndrome.	in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ...	1998	9501041
urine-associated horizontal transmission of seoul virus among rats.	to understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with the ki-83-262 strain of seoul virus, s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ...	1998	9505963
severe hantavirus pulmonary syndrome: a new indication for extracorporeal life support?		1998	9468156
hantavirus pulmonary syndrome--colorado and new mexico, 1998.	hantavirus pulmonary syndrome (hps) is a severe cardiopulmonary illness resulting in death in approximately 45% of cases. the most frequently recognized etiologic agent of hps in north america, sin nombre virus (snv), is transmitted to humans from its primary rodent reservoir, peromyscus maniculatus (deer mouse), by direct contact with infected rodents, rodent droppings, or nests or through inhalation of aerosolized virus particles from mouse urine and feces. sporadic cases occur throughout the ...	1998	9639364
genetic diversity and epidemiology of hantaviruses in argentina.	phylogenetic analysis of a 292-nucleotide (nt) fragment of the hantavirus m genome segment from 36 rodent and 13 human samples from three known foci of hantavirus infection in argentina was conducted. a 1654-nt fragment of the m genome segment was analyzed for 1 representative of 7 genetically distinct hantavirus lineages identified. additionally, the nt sequence of the complete m genome segments of lechiguanas, oran, and hu39694 hantavirus genotypes was determined. nt sequence comparisons revea ...	1998	9498428
hantavirus pulmonary syndrome outbreak in argentina: molecular evidence for person-to-person transmission of andes virus.	an increase of hantavirus pulmonary syndrome (hps) cases around a southwestern argentina town and in persons living 1400 km away but in contact with those cases was detected during the spring of 1996. in order to evaluate person-to-person transmission we compared the homology of pcr-amplified viral sequences of 26 argentine and chilean cases. sixteen of them were epidemiologically linked cases and had the same sequence (epilink/96) in the s segment 3' noncoding region and in the m segment partia ...	1998	9499807
haemorrhagic fever with renal syndrome caused by puumala virus: evaluation of the risk for cataract formation.		1998	9614533
genetic analysis of the diversity and origin of hantaviruses in peromyscus leucopus mice in north america.	nucleotide sequences were determined for the complete m genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and pcr-positive white-footed mice (peromyscus leucopus) from indiana and oklahoma. phylogenetic analyses indicated that although divergent from each other, the virus lineages in indiana and oklahoma were monophyletic and formed a newly identified unique ancestral branch within the clade of sin nombre-like viruses found in peromyscus mice ...	1998	9420200
severe thrombocytopenia and response to corticosteroids in a case of nephropathia epidemica.	nine days after working in the woods, a previously healthy 32-year-old man fell seriously ill. his symptoms included high fever, chills, diffuse myalgia, severe headache, and back pain. on the fifth day of onset of symptoms, blood tests showed creatinine levels of 5.4 mg/dl accompanied by marked proteinuria. after admission to the hospital, a diagnosis of nephropathia epidemica (ne) caused by puumala virus was made using solid-phase enzyme-linked immunosorbent assay (elisa). the patient graduall ...	1998	9428461
hantavirus pulmonary syndrome.		1998	9493883
human recombinant puumala virus antibodies: cross-reaction with other hantaviruses and use in diagnostics.	a panel of seven human monoclonal fabs against puumala virus (puu) nucleocapsid protein (n) was obtained by panning an antibody phage-display library prepared from the spleen of a puu-immune individual. three antibodies reacted in immunoblotting and cross-reacted strongly with tula and sin nombre virus recombinant n proteins. these antibodies mapped to the amino terminus of the n protein. one puu glycoprotein 2 (g2)-specific fab obtained against a novel epitope (g2c) cross-reacted with khabarovs ...	1998	9568958
central nervous system and ophthalmic involvement in nephropathia epidemica (european type of haemorrhagic fever with renal syndrome).	central nervous system (cns) - related symptoms occur in haemorrhagic fever with renal syndrome (hfrs). to study the cns and ophthalmic involvement in nephropathia epidemica (ne), the european type of hfrs, we included 26 patients in a prospective study. most common cns-related symptoms were headache (96%), insomnia (83%), vertigo (79%), nausea (79%), and vomiting (71%). ophthalmic symptoms were reported by 82% of patients; 41% had photophobia and 50% had impaired vision. a transient loss of vis ...	1998	9570645
hantavirus infection: a possible cause of delayed sensorineural hearing loss.		1998	9591876
adjuvant activity of muramyl dipeptide derivatives to enhance immunogenicity of a hantavirus-inactivated vaccine.	the adjuvant effect of two lipophilic derivatives of muramyl dipeptide (mdp), b30-mdp and mdp-lys(l18), on the ability of an inactivated vaccine of b-1 virus (b-1 vaccine) to induce immune response against hantavirus causing hemorrhagic fever with renal syndrome (hfrs) was examined. when mice were immunized subcutaneously (s.c.) twice at 2-week intervals with b-1 vaccine admixed with or without 100 micrograms mouse-1 of b30-mdp (b-1/b30-mdp) or mdp-lys(l18) [b-1/mdp-lys(l18)], mice immunized wit ...	1998	9607033
a miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers.	in this paper, we describe a miniature analytical thermal cycling instrument (matci) to amplify and detect dna via the polymerase chain reaction in real-time. the matci is an integrated, miniaturized analytical system that uses silicon-based, high-efficiency reaction chambers with integrated heaters and simple, inexpensive electronics to precisely control the reaction temperatures. optical windows in the silicon and solid-state, diode-based detection components are employed to perform real-time ...	1998	9511467
southwest cautious over hantavirus outbreak.		1998	9713524
viral hemorrhagic fevers and hantavirus infections in the americas.	several arenaviruses and hantaviruses have been isolated in the americas during the last 4 decades. these are rodent-borne viruses responsible for the south american hemorrhagic fevers (sahf) and hantavirus pulmonary syndrome (hps). although rare, sahf and hps are serious illnesses with high mortality rates. most viral isolates found in the americas represent new world lineages of their respective viral families. their presence in the western hemisphere is likely ancient, their relationship with ...	1998	9494832
human dobrava hantavirus infections in estonia.	outbreaks of severe hemorrhagic fever with renal syndrome (hfrs) caused by dobrava hantavirus (dob) have been reported in albania, greece, and bosnia-herzegovina. a seroepidemiologic study conducted by the authors in estonia provided the first evidence of human dob infection in a baltic country. hantavirus infection was first confirmed in estonia in 1986 and believed related to puumala hantavirus (puu). to enable precise serotyping, serum samples were collected from 1728 healthy people in 11 ...	1998	9717928
hantavirus pulmonary syndrome: implications for critical care nurses.		1998	9515465
tissue distribution of hantavirus antigen in naturally infected humans and deer mice.	the sin nombre virus (snv) is the etiologic agent of hantavirus pulmonary syndrome in humans but does not cause disease in chronically infected deer mice (peromyscus maniculatus), the natural host. in this study, murine antiserum raised against recombinant snv nucleocapsid protein was utilized to localize viral antigen immunohistochemically in tissues from both humans (n = 20; 11 positive, 9 negative) and deer mice (n = 6; 4 positive, 2 negative). viral infection status was confirmed by western ...	1998	9607851
association of hla b27 with benign clinical course of nephropathia epidemica caused by puumala hantavirus.	both the severe course of nephropathia epidemica (ne) caused by puumala hantavirus, and the fast progression of human immunodeficiency virus (hiv) disease, are associated with the hla b8 drb1*0301 haplotype. as hla b27, on the contrary, is associated with the slow progression of hiv disease, we wanted to test whether the same is true for ne. only six (8%) ne patients, half the figure expected, had the hla b27 allele in 74 randomly selected hospital-treated patients. all six had a benign overall ...	1998	9519867
hantavirus infection.		1998	9520902
role of actin microfilaments in black creek canal virus morphogenesis.	we have investigated the involvement of cytoskeletal proteins in the morphogenesis of black creek canal virus (bccv), a new world hantavirus. immunofluorescent staining of bccv-infected cells revealed a filamentous pattern of virus antigen, the appearance of which was sensitive to treatment with cytochalasin d, an actin microfilament-depolymerizing drug. double immunofluorescence staining of bccv-infected vero cells with anti-bccv nucleocapsid (n) monoclonal antibody and phalloidin revealed a co ...	1998	9525606
neuropsychological impairments following hantavirus pulmonary syndrome.	recently an outbreak of acute respiratory infection associated with the hantavirus occurred in the southwestern united states. hantavirus pulmonary syndrome (hps) is a life threatening illness that carries with it a high mortality rate. patients with hps experience prolonged periods of hypoxemia requiring mechanical ventilation and treatment in intensive care units. we have recently seen 2 survivors of hps. a neuropsychological test battery was administered immediately following their acute hosp ...	1998	9529829
urine-associated horizontal transmission of seoul virus among rats.	to understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with ki-83-262 strain of seoul virus, the s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ...	1998	9541619
of mice and mostly men--hantavirus pulmonary syndrome.		1998	9559018
beta3 integrins mediate the cellular entry of hantaviruses that cause respiratory failure.	newly emerged hantaviruses replicate primarily in the pulmonary endothelium, cause acute platelet loss, and result in hantavirus pulmonary syndrome (hps). we now report that specific integrins expressed on platelets and endothelial cells permit the cellular entry of hps-associated hantaviruses. infection with hps-associated hantaviruses, ny-1 and sin nombre virus (snv), is inhibited by antibodies to beta3 integrins and by the beta3-integrin ligand, vitronectin. in contrast, infection with the no ...	1998	9618541
glomerular basement membrane antibodies in hantavirus disease: any pathogenetic implication?		1998	9617503
chimaeric hbv core particles carrying a defined segment of puumala hantavirus nucleocapsid protein evoke protective immunity in an animal model.	hantaviruses are rodent-born agents which are pathogenic in humans causing haemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. to induce a protective immunity against a european hantavirus (puumala) we constructed chimaeric hepatitis b virus (hbv) core particles carrying defined fragments of the puumala virus nucleocapsid protein. after immunisation of bank voles, the natural host of puumala virus, with core particles possessing an insertion of the n-terminal part of puumala ...	1998	9607042
evaluation of puumala virus igg and igm enzyme immunoassays based on recombinant baculovirus-expressed nucleocapsid protein for early nephropathia epidemica diagnosis.	puumala virus (puu), a member of hantavirus genus, is the causative agent of nephropathia epidemica (ne), a milder form of hemorrhagic fever with renal syndrome (hfrs). rapid diagnosis is essential for clinical management of ne.	1998	9646005
short report: decrease in seroprevalence of antibodies to hantavirus in rodents from 1993-1994 hantavirus pulmonary syndrome case sites.	rodent trapping was conducted at seven hantavirus pulmonary syndrome (hps) case sites from june 1993 to march 1994 during the hps outbreak in the southwestern united states. to determine if there were changes in the rodent population or the hantavirus seroprevalence in rodents since the hps outbreak, rodents were trapped at the same sites three years later using the same trapping protocol. the trap success decreased from the numbers trapped during the outbreak, however, the number of peromyscus, ...	1998	9660455
retrospective serological and genetic study of the distribution of hantaviruses in greece.	a retrospective serological and genetic study of hantaviruses responsible for hemorrhagic fever with renal syndrome (hfrs) in greece during the last 17 years is presented. fifty-one serum samples taken from 30 hfrs cases previously diagnosed by immunofluorescence assay were tested by elisa for igg (hantaan, dobrava, and puumala) and igm antibodies (hantaan and puumala). results were compatible with the majority of infections being related to hantaviruses carried by rodents of the subfamily murin ...	1998	9661842
first reported case of hantavirus pulmonary syndrome in oklahoma.	hantavirus pulmonary syndrome (hps) is a condition of rapidly progressive pulmonary failure with a case-fatality rate of almost 50 percent. rodents serve as the reservoir for hantaviruses and human infection occurs primarily via aerosolized virus in rodent excreta. the rodent reservoir for the disease is widespread across oklahoma and the first case of hps has recently been confirmed in an oklahoma resident. physicians should suspect hps in a previously healthy person who develops a febrile illn ...	1998	9763765
distinguishing hantavirus pulmonary syndrome from acute respiratory distress syndrome by chest radiography: are there different radiographic manifestations of increased alveolar permeability?	hantavirus infection may cause diffuse air space disease, termed hantavirus pulmonary syndrome (hps). the authors sought to determine if chest radiographs could differentiate hps from typical acute respiratory distress syndrome (ards). the authors identified patients with either hps (n = 11) or acute ards (n = 32) and selected the earliest chest radiograph showing diffuse airspace disease, and a chest radiograph taken 24 to 48 hours previously. thoracic and general radiologists first viewed the ...	1998	9671418
[in the month of may, do whatever you please...].		1998	9775088
acute exacerbation of autoimmune liver disease associated with hantaviral infection.	hantavirus is known to cause haemorrhagic fever with renal syndrome (hfrs). although liver dysfunction has been reported in hfrs, hepatic manifestations of hantaviral infection have not been well described. we describe a case of autoimmune cholangitis in which an exacerbation of hepatitis was associated with hantaviral infection. seroconversion of both igg- and igm-class antibodies to hantavirus was noted coincident with acute exacerbation of hepatitis, which was resolved promptly by treatment w ...	1998	9670364
[anti-hantavirus antibodies in school children in salvador, bahia].	hantavirosis are emerging diseases in the americas. although considered rare in children, among the five cases diagnosed in brazil, one was in this age group. to know the serum-prevalence of hantavirosis in the pediatric group (schoolchildren) of low social economic level in salvador, bahia state, and to associate demographic, socio-economic and environmental factors to the serologic results, a standard questionnaire was applied and blood samples were collected from 379 students of two public sc ...	1998	9789441
a major outbreak of hemorrhagic fever with renal syndrome in the samara region, european russia.		1998	9795799
hantavirus infection in the four corners region of usa in 1998.		1998	9802275
isolation and characterization of puumala hantavirus from norway: evidence for a distinct phylogenetic sublineage.	puumala (puu) hantavirus is the aetiological agent of nephropathia epidemica (ne), a mild form of haemorrhagic fever with renal syndrome, which occurs in fennoscandia, central europe and russia. in norway, ne-like disease has been reported since 1946 and about 50 cases are diagnosed annually; however, the causative agent has not been characterized. in this study, a virus originating from bank voles (clethrionomys glareolus) trapped near the town of eidsvoll (akershus county) was isolated and pas ...	1998	9820135