Publications

hantaviral infections of rodents: possible scenarios.		2009	19568690
a single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus gn glycoprotein confers protective immunity against seoul virus in mice.	seoul virus (seov), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) and afflicts tens of thousands of people annually. in this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gn protein of seov (rcav-2-gn) in balb/c mice. sera from immunized mice contained neutralizing antibodies that could specifically recognize seov and neutralize its infectivity in vitro. ...	2009	19583957
evolutionary insights from a genetically divergent hantavirus harbored by the european common mole (talpa europaea).	the discovery of genetically distinct hantaviruses in shrews (order soricomorpha, family soricidae) from widely separated geographic regions challenges the hypothesis that rodents (order rodentia, family muridae and cricetidae) are the primordial reservoir hosts of hantaviruses and also predicts that other soricomorphs harbor hantaviruses. recently, novel hantavirus genomes have been detected in moles of the family talpidae, including the japanese shrew mole (urotrichus talpoides) and american s ...	2009	19582155
using satellite images of environmental changes to predict infectious disease outbreaks.	recent events clearly illustrate a continued vulnerability of large populations to infectious diseases, which is related to our changing human-constructed and natural environments. a single person with multidrug-resistant tuberculosis in 2007 provided a wake-up call to the united states and global public health infrastructure, as the health professionals and the public realized that today's ease of airline travel can potentially expose hundreds of persons to an untreatable disease associated wit ...	2009	19788799
hantavirus infection in the republic of georgia.	we describe a laboratory-confirmed case of hantavirus infection in the republic of georgia. limited information is available about hantavirus infections in the caucasus, although the infection has been reported throughout europe and russia. increasing awareness and active disease surveillance contribute to our improved understanding of the geographic range of this pathogen.	2009	19788822
hantavirus pulmonary syndrome in brasilia periphery: a diagnostic challenge.	hantavirus pulmonary syndrome (hps) is an emerging rodent-borne disease in the american continent, characterized by acute respiratory distress and a high case-fatality ratio. the present work describes a case of hps, with favorable outcome, whose initial features were mistaken for leptospirosis or other less severe acute infections.	2009	19801809
serological microarray for detection of hsv-1, hsv-2, vzv, and cmv antibodies.	the seroprevalence of human herpesviruses is high and reactivations occur frequently. a microarray was designed and tested for the detection of igg and igm antibodies for puumala hantavirus (puuv) and igg antibodies against four herpesviruses. initially, a microarray platform was set up using an unrelated in-house antigen, puuv recombinant nucleocapsid protein, to optimize the protocol for the detection of antibodies. detection of the four herpesviruses was set up in a microarray using the recom ...	2009	19477202
[the first french hemorrhagic fever with renal syndrome in pregnant woman].	the seroprevalence of hantavirus in france is very low. the hantavirus is the causative agent of hemorrhagic fever with renal syndrome (hfrs). in 2005, we report the first french hfrs case in a pregnant woman presenting with influenza like symptoms in an endemic area associated with suggestive biologic abnormalities. she was delivered by emergency cesarean section. her child had a neonatal respiratory distress, thrombocytopenia and elevated liver enzymes levels, which suggest a transplacental ha ...	2009	19497685
phage display selection of cyclic peptides that inhibit andes virus infection.	specific therapy is not available for hantavirus cardiopulmonary syndrome caused by andes virus (andv). peptides capable of blocking andv infection in vitro were identified using antibodies against andv surface glycoproteins gn and gc to competitively elute a cyclic nonapeptide-bearing phage display library from purified andv particles. phage was examined for andv infection inhibition in vitro, and nonapeptides were synthesized based on the most-potent phage sequences. three peptides showed leve ...	2009	19515773
molecular diagnostics of hemorrhagic fever with renal syndrome during a dobrava virus infection outbreak in the european part of russia.	a large outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred in the winter of 2006-2007 in a region southeast of moscow in central european russia. of the 422 patients with hfrs investigated in this study, 58 patients were found to be infected by puumala virus, whereas as many as 364 were infected by dobrava-belgrade virus (dobv). early serum samples from 10 dobv-infected patients were used for nucleic acid amplification, which was successful for 5 patients. molecular analyses demon ...	2009	19828747
theory of possible effects of the allee phenomenon on the population of an epidemic reservoir.	we investigate possible effects of high-order nonlinearities on the shapes of infection refugia of the reservoir of an infectious disease. we replace fisher-type equations that have been recently used to describe, among others, the hantavirus spread in mouse populations by generalizations capable of describing allee effects that are a consequence of the high-order nonlinearities. after analyzing the equations to calculate steady-state solutions, we study the stability of those solutions and comp ...	2009	19518251
testing mechanisms of the dilution effect: deer mice encounter rates, sin nombre virus prevalence and species diversity.	species diversity has been shown to decrease prevalence of disease in a variety of host-pathogen systems, in a phenomenon termed the dilution effect. several mechanisms have been proposed by which diversity may decrease prevalence, though few have been tested in natural host-pathogen systems. we investigated the mechanisms by which diversity influenced the prevalence of sin nombre virus (snv), a directly transmitted virus in deer mice (peromyscus maniculatus). we monitored both intra and intersp ...	2009	19495881
knowledge and practices about hantavirus pulmonary syndrome in a cluster of japanese communities in argentina.	to provide information on hantavirus pulmonary syndrome (hps) knowledge and practices among a cluster of the rural japanese communities near la plata in central argentina, an area with confirmed hps cases. particular emphasis was placed on testing the hypothesis that there would be differences between household use of hps prevention measures based on the head-of-household's knowledge of hps.	2009	19531307
[climate changes and emerging diseases. what new infectious diseases and health problem can be expected?].	increasing temperatures, but also other climatic factors, will have an impact on human health. apart from the direct consequences of extreme weather conditions (e.g., heat-related fatalities), indirect health consequences in the long-term are also of great importance. in addition to a likely increase in allergic diseases and additional complications in the course of cardiovascular and respiratory diseases, infectious diseases are of particular interest. in germany, endemic pathogens, such as han ...	2009	19536444
co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary.	hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ...	2009	19856478
[infectious diseases and climate change].	climate changes will likely have an impact on the spectrum of infectious diseases in europe. we may see an increase in vector-borne diseases, diseases spread by rodents such as hantavirus, and food- and water-borne diseases. as the effects of climate changes are likely to occur gradually, a modern industrialised country such as denmark will have the opportunity to adapt to the expected changes.	2009	19857396
young man with kidney failure and hemorrhagic interstitial nephritis.		2009	19556044
seasonal variation in sin nombre virus infections in deer mice: preliminary results.	the proportion of deer mice (peromyscus maniculatus) with recently acquired sin nombre virus (snv) infections is an indicator of epizootic intensity and may be key in predicting outbreaks of hantavirus cardio-pulmonary syndrome in humans. we investigated whether incidence of recent infections was related to season, sex, reproductive status, or habitat disturbance. in may and september, 2006, we sampled 912 deer mice at six sites in utah. we determined snv antibody prevalence and estimated the nu ...	2009	19395752
[survey on host animal and molecular epidemiology of hantavirus in chuxiong prefecture, yunnan province].	to determine the hosts of hantavirus (hv) and its molecular epidemiological characteristics, to provide evidence for prevention and control on hemorrhagic fever with renal syndrome (hfrs).	2009	19642377
replication reduction neutralization test, a quantitative rt-pcr-based technique for the detection of neutralizing hantavirus antibodies.	hantaviruses, which are mainly rodent-borne viruses, cause hemorrhagic fever with renal syndrome in the old world, and hantavirus pulmonary syndrome in the new world. a neutralization test based on quantitative rt-pcr, the replication reduction neutralization test (rrnt), was developed for efficient detection of hantavirus-neutralizing antibodies. the effectiveness of the rrnt was evaluated by examining several hantaviruses and hantavirus-specific convalescent human serum samples. all convalesce ...	2009	19406158
first molecular evidence of tula hantavirus in microtus voles in slovenia.	different microtus species, present in a worldwide range habitat populating north america, europe, asia, and few other species have been recognized previously as a hantavirus reservoir. tula hantavirus was first reported in microtus arvalis and microtus rossiaemeridionalis from central russia and later discovered in several european countries. using molecular techniques we have demonstrated the presence of tula hantavirus in three different microtus species in slovenia. phylogenetic analyses of ...	2009	19410611
experimental evidence for reduced rodent diversity causing increased hantavirus prevalence.	emerging and re-emerging infectious diseases have become a major global environmental problem with important public health, economic, and political consequences. the etiologic agents of most emerging infectious diseases are zoonotic, and anthropogenic environmental changes that affect wildlife communities are increasingly implicated in disease emergence and spread. although increased disease incidence has been correlated with biodiversity loss for several zoonoses, experimental tests in these sy ...	2009	19421313
[pulmonary and cardiovascular syndrome due to hantavirus: clinical aspects of an emerging disease in southeastern brazil].	pulmonary and cardiovascular syndrome due to hantavirus is a disease caused by inhalation of aerosols from the excreta of wild rodents contaminated by viruses of the bunyaviridae family. we studied the clinical and laboratory manifestations of 70 cases that occurred in the region of ribeirão preto, sp, brazil, between 1998 and 2007. the frequency of symptoms was as follows: dyspnea (87%), fever (81%), coughing (44%), headache (34%), tachycardia (81%), low arterial blood pressure (56%), metabolic ...	2009	19684976
tubular proteinuria and glomerular filtration 6 years after puumala hantavirus-induced acute interstitial nephritis.	we previously found increased urinary protein excretion, glomerular filtration rate (gfr) and blood pressure in a retrospective analysis of patients with previous nephropathia epidemica (ne). here, we evaluated the long-term outcome after ne in a prospectively recruited patient group.	2009	19390211
hantavirus pulmonary syndrome in texas: 1993-2006.	hantavirus pulmonary syndrome (hps) is a rare cardiopulmonary disease that was first described after a 1993 epidemic in the southwestern united states. this study reviewed all cases reported in texas to date.	2009	19077779
contact heterogeneity in deer mice: implications for sin nombre virus transmission.	heterogeneities within disease hosts suggest that not all individuals have the same probability of transmitting disease or becoming infected. this heterogeneity is thought to be due to dissimilarity in susceptibility and exposure among hosts. as such, it has been proposed that many host-pathogen systems follow the general pattern whereby a small fraction of the population accounts for a large fraction of the pathogen transmission. this disparity in transmission dynamics is often referred to as ' ...	2009	19129136
predicting the risk of hantavirus infection in beijing, people's republic of china.	to understand the spatial distribution of hantavirus infections across landscapes and its influencing environment factors in beijing, people's republic of china, rodents captured in this region were examined for hantavirus by reverse transcription-polymerase chain reaction. a total of 1,639 rodents were trapped at 86 randomly selected sites. the overall infection rate for hantavirus was 7.14% in the rodents. multivariate logistic regression analysis indicated that the natural infection rates for ...	2009	19346399
hantavirus infection and habitat associations among rodent populations in agroecosystems of panama: implications for human disease risk.	hantavirus cardiopulmonary syndrome (hcps), which is caused by infection with choclo virus, is uncommon in panama, yet seropositivity among rural residents is as high as 60%. to clarify the environmental risk factors favoring rodent-to-human transmission, we tested serum from 3,067 rodents captured over a five-year period for antibodies against recombinant n protein of hantavirus by enzyme immunoassay and strip immunoblot. among 220 seropositive rodents, oligoryzomys fulvescens, the reservoir of ...	2009	19556568
phylogenetic characterization of hantaviruses from wild rodents and hantavirus pulmonary syndrome cases in the state of parana (southern brazil).	over 1,100 cases of hantavirus pulmonary syndrome (hps) have occurred in brazil since 1993, but little is known about brazilian hantaviruses, and many of their rodent hosts remain unknown. the araucaria hantavirus (arauv) was described recently from hps patients from paraná, in southern brazil, but its host could not be identified. in this study, rodents were captured from regions with high hps prevalence to address this issue. arauv rna was detected in three distantly related rodent species: ol ...	2009	19439554
[serological characteristics of hantaviruses from clinical specimens analyzed in 1998-2008 in the department of virology, public health institute, ostrava].	in 1998 - 2008, serum samples from 464 patients, predominantly residents of the moravia-silesian region, were examined in the department of virology, public health institute in ostrava, using enzyme-linked immunosorbent assay (elisa) for the detection of specific anti-hantavirus antibodies. seropositivity was ascertained in 41 persons (8.8%). more patients tested seropositive against hantaan virus (24, i.e. 58.5%) than against puumala virus (13, i.e. 31.7%). four persons were reactive against bo ...	2009	19750821
[cloning and expression of the nucleoprotein gene of puumala-like virus].	in order to detect hokkaido virus (hokv), a recombinant baculovirus containing the nucleoprotein (np) gene of hokv was constructed, and then the np was expressed in insect cell.	2009	19565881
[study on the difference of genes and the type identification of hantavirus from lishui, zhejiang province].	to isolate hantavirus from lishui county--one of the epidemic regions for hemorrhagic fever with renal syndrome (hfrs), in zhejiang province, and to identify the serotype and molecular/biological characteristics of a new htn subtype hantavirus (hv) strains, hopefully to provide evidence for hfrs prevention and therapy.	2009	19565882
[purpura fulminans, cerebral infarcts and multiorganic dysfunction due to hantavirus infection. review and case report].	multiorganic failure is a rare manifestation of hantavirus infection but it should be included among differential diagnoses of multiorganic failure in the pediatric age. currently, there is no effective therapy for this infection. a high suspicion index and early referral to a pediatric intensive care unit with extracorporeal membrane oxygenation may lead to a favourable impact in the outcome. we report a fatal case of hantavirus infection in a 9-year-old girl with a severe disease refractory to ...	2009	19350147
the first established focus of hantavirus infection in poland, 2007.	the first hantavirus infection outbreak in poland (with different seroetiology) was identified between august-december 2007. thirteen cases were reported in southeast poland: 12 cases in the carpathians bordering with northeast slovakia, mainly in the forested areas of the bieszczady mountains, and one case approximately 100-120 kilometres north from the others, in the adjacent sub-carpathian region. four additional cases of past infection were identified retrospectively, based on the presence o ...	2009	19572481
[diagnosis and treatment of cardiopulmonary hantavirus syndrome: chile-2007].		2009	19350164
characterization of imjin virus, a newly isolated hantavirus from the ussuri white-toothed shrew (crocidura lasiura).	until recently, the single known exception to the rodent-hantavirus association was thottapalayam virus (tpmv), a long-unclassified virus isolated from the asian house shrew (suncus murinus). robust gene amplification techniques have now uncovered several genetically distinct hantaviruses from shrews in widely separated geographic regions. here, we report the characterization of a newly identified hantavirus, designated imjin virus (mjnv), isolated from the lung tissues of ussuri white-toothed s ...	2009	19357167
hantavirus infection: a case report from india.	the clinical presentation of hantavirus infections in india is unclear. we report here a case of hantavirus infection in a 46 year old quarry worker presenting with fever, abdominal pain, jaundice, thrombocytopenia and renal dysfunction. seroconversion and rising anti-hantavirus igg titers were taken as evidence of hantavirus infection. clinicians should consider hantavirus infections in the differential diagnosis of acute febrile illness along with scrub typhus, leptospirosis and dengue.	2009	19584514
analysis of puumala hantavirus in a bank vole population in northern finland: evidence for co-circulation of two genetic lineages and frequent reassortment between strains.	in this study, for the first time, two distinct genetic lineages of puumala virus (puuv) were found within a small sampling area and within a single host genetic lineage (ural mtdna) at pallasjärvi, northern finland. lung tissue samples of 171 bank voles (myodes glareolus) trapped in september 1998 were screened for the presence of puuv nucleocapsid antigen and 25 were found to be positive. partial sequences of the puuv small (s), medium (m) and large (l) genome segments were recovered from thes ...	2009	19386780
temporal and geographic evidence for evolution of sin nombre virus using molecular analyses of viral rna from colorado, new mexico and montana.	all viruses in the family bunyaviridae possess a tripartite genome, consisting of a small, a medium, and a large rna segment. bunyaviruses therefore possess considerable evolutionary potential, attributable to both intramolecular changes and to genome segment reassortment. hantaviruses (family bunyaviridae, genus hantavirus) are known to cause human hemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. the primary reservoir host of sin nombre virus is the deer mouse (peromyscus ...	2009	19602267
hantaviruses and climate change.	most hantaviruses are rodent-borne emerging viruses. they cause two significant human diseases, haemorrhagic fever with renal syndrome in asia and europe, and hantavirus cardiopulmonary syndrome in the americas. very recently, several novel hantaviruses with unknown pathogenic potential have been identified in africa and in a variety of insectivores (shrews and a mole). because there is very limited information available on the possible impact of climate change on all of these highly dangerous p ...	2009	19604276
hantavirus reservoir oligoryzomys longicaudatus spatial distribution sensitivity to climate change scenarios in argentine patagonia.	oligoryzomys longicaudatus (colilargo) is the rodent responsible for hantavirus pulmonary syndrome (hps) in argentine patagonia. in past decades (1967-1998), trends of precipitation reduction and surface air temperature increase have been observed in western patagonia. we explore how the potential distribution of the hantavirus reservoir would change under different climate change scenarios based on the observed trends.	2009	19607707
hanta virus infection during dengue virus infection outbreak in indonesia.	to investigate which recent infection could have caused the present dengue-like symptoms, in adult patients clinically fulfilling the who criteria for dengue, in which serologically were not confirmed for dengue virus infections.	2009	19390126
increased host species diversity and decreased prevalence of sin nombre virus.	emerging outbreaks of zoonotic diseases are affecting humans at an alarming rate. until the ecological factors associated with zoonoses are better understood, disease emergence will continue. for lyme disease, disease suppression has been demonstrated by a dilution effect, whereby increasing species diversity decreases disease prevalence in host populations. to test the dilution effect in another disease, we examined 17 ecological variables associated with prevalence of the directly transmitted ...	2009	19624913
a proposal for new criteria for the classification of hantaviruses, based on s and m segment protein sequences.	hantaviruses, members of the family bunyaviridae, are the causative agents of hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. hantaviruses are currently demarcated into species based on the guidelines provided by the international committee on taxonomy of viruses (ictv). these guidelines however, are often ignored by the descriptors of novel hantaviruses. with this study we attempted to refine the second ictv guideline for hantavirus species demarcation by phylogen ...	2009	19393771
host switch during evolution of a genetically distinct hantavirus in the american shrew mole (neurotrichus gibbsii).	a genetically distinct hantavirus, designated oxbow virus (oxbv), was detected in tissues of an american shrew mole (neurotrichus gibbsii), captured in gresham, oregon, in september 2003. pairwise analysis of full-length s- and m- and partial l-segment nucleotide and amino acid sequences of oxbv indicated low sequence similarity with rodent-borne hantaviruses. phylogenetic analyses using maximum-likelihood and bayesian methods, and host-parasite evolutionary comparisons, showed that oxbv and asa ...	2009	19394994
evidence of circulation of laguna negra-like hantavirus in the central west of brazil: case report.	hantavirus pulmonary syndrome has been reported with increasing frequency in some brazilian regions, but information about viral genetic identification is still limited. recently, the state of mato grosso, in the legal amazon of brazil, experienced a growing number of hantavirus pulmonary syndrome (hps) cases but the genetic characterization of the causative hantavirus is still missing.	2009	19395308
a newly recognized hantavirus in the asian house rat (rattus tanezumi) in indonesia.	hantaviral sequences were recovered from the lung tissue of an asian house rat (rattus tanezumi) captured in serang, indonesia. phylogenetic analysis of partial l, m and s segment sequences showed that they belonged to a novel hantavirus provisionally named serang virus (serv). notably, serv is distinct from the hantaviruses associated with rodents of the species rattus: seoul virus associated with rattus norvegicus worldwide and gou virus isolated from rattus rattus in china. instead serv appea ...	2009	19088290
the hantaviral load in tissues of naturally infected rodents.	hantaviruses cause a lifelong and asymptomatic infection in naturally infected hosts as well as in experimentally infected rodents. understanding the ecology and pathogenesis of hantaviruses requires an interdisciplinary research approach, which links laboratory experiments with results gained from field studies. although several studies report hantavirus persistence and tissue infection patterns for experimentally infected rodents, field data is very limited. for this reason, the aim of our stu ...	2009	19397875
adenovirus vectors expressing hantavirus proteins protect hamsters against lethal challenge with andes virus.	hantaviruses infect humans following aerosolization from rodent feces and urine, producing hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. due to the high rates of mortality and lack of therapies, vaccines are urgently needed. nonreplicating adenovirus (ad) vectors that express andes hantavirus (andv) nucleocapsid protein (adn) or glycoproteins (adg(n) and adg(c)) were constructed. ad vectors were tested for their ability to protect syrian hamsters from a lethal andv inf ...	2009	19403663
central nervous system complications following hanta virus cardiopulmonary syndrome.		2009	19089788
characterization of hantaviruses circulating in central brazil.	in 2004, an outbreak of hcps in brazil made hantaviruses a national threat to the rural and urban population. during this outbreak, 164 cases were reported, and 18.3% of them occurred in the federal district. in this study, hantavirus genomic sequences were amplified from seven patients who resided in central brazil and then sequenced and compared to other hantavirus sequences. the complete s segment sequence, which is 1847 bases long and potentially encodes the 428 amino acid nucleocapsid prote ...	2009	19103306
ecology, genetic diversity, and phylogeographic structure of andes virus in humans and rodents in chile.	andes virus (andv) is the predominant etiologic agent of hantavirus cardiopulmonary syndrome (hcps) in southern south america. in chile, serologically confirmed human hantavirus infections have occurred throughout a wide latitudinal distribution extending from the regions of valparaíso (32 to 33 degrees s) to aysén (46 degrees s) in southern patagonia. in this study, we found seropositive rodents further north in the coquimbo region (30 degrees s) in chile. rodent seroprevalence was 1.4%, with o ...	2009	19116256
is hantavirus important to practitioners?		2009	19434032
hantavirus pulmonary syndrome: a concise clinical review.	in 1978, hantaviruses were first described as the etiological agent of hemorrhagic fever with renal syndrome (hfrs) in korea. since then, numerous related, enveloped, negative-stranded rna viruses have been identified, forming the genus hantavirus within the family bunyaviridae. these pathogens are distributed worldwide and thus can be classified, on the basis of phylogenetic origins, into old world viruses or new world viruses (ie north, central, and south america). similarly, these viruses cau ...	2009	19434035
hantaan virus triggers tlr3-dependent innate immune responses.	immediately after viral infection, innate responses including expression of ifn-alpha/beta and ifn-stimulated genes (isgs) are elicited ubiquitously by recruitment of specific pathogen recognition receptors. the velocity to induce ifn-alpha/beta and isgs in response to an infection is often decisive for virulence. interestingly, in primary endothelial cells isgs are induced later by hantaviruses pathogenic to humans than those considered to be nonpathogenic or of low virulence. here we demonstra ...	2009	19234180
association between habitat and prevalence of hantavirus infections in bank voles (myodes glareolus) and wood mice (apodemus sylvaticus).	in order to determine the habitat preferred by myodes (before clethrionomys) glareolus and the corresponding puumala hantavirus seroprevalence in those habitats, we captured rodents simultaneously in three significantly different habitats. we compared trapping success and presence of virus per habitat during an ongoing epidemic in order to test the hypothesis of a density-dependent seroprevalence. our study showed that bank vole population density, as well as puumala virus seroprevalence, were h ...	2009	19271997
genetic diversity and geographic distribution of hantaviruses in russia.	haemorrhagic fever with renal syndrome (hfrs) is the most prevalent zoonotic disease in russia. it is caused by several hantavirus species hosted by small rodents. we describe spatial and temporal patterns of hfrs incidence in the russian federation, and the geographic distribution of prevalent hantavirus species: puumala (puuv) and dobrava (dobv). partial sequencing of nucleocapsid and glycoprotein genes of 117 puuv strains and 78 dobv strains revealed several distinct genetic subgroups. the rn ...	2009	19486318
plagues in the icu: a brief history of community-acquired epidemic and endemic transmissible infections leading to intensive care admission.	the ability to diagnose and treat infectious diseases and handle infectious disease outbreaks continues to improve. for the most part, the major plagues of antiquity remain historical footnotes, yet, despite many advances, there is clear evidence that major pandemic illness is always just one outbreak away. in addition to the hiv pandemic, the smaller epidemic outbreaks of legionnaire's disease, hantavirus pulmonary syndrome, and severe acute respiratory syndrome, among many others, points out t ...	2009	19268795
seoul virus and hantavirus disease, shenyang, people's republic of china.	an outbreak of hemorrhagic fever with renal syndrome (hfrs) occurred among students in shenyang pharmaceutical university in 2006. we conducted a study to characterize etiologic agents of the outbreaks and clarify the origin of hantaviruses causing infections in humans and laboratory animals. immunoglobulin (ig) m or igg antibodies against seoul virus (seov) were detected in the serum samples of all 8 patients. igg antibodies against hantavirus were also identified in laboratory rats, which were ...	2009	19193263
diagnostic aspects for epidemic hemorrhagic fever in legal medical autopsy: report of 2 cases and review.	epidemic hemorrhagic fever (ehf) is known also as hemorrhagic fever with renal syndrome (hfrs). hantaviruses, a notorious cause of ehf is spreaded by rodents or biting insects, can cause epidemic hemorrhagic fever. this report describes two rare medico-legal autopsy cases died from epidemic hemorrhagic fever. the victims were two male individuals aged 34 years and 29 years. the medico-legal autopsies were requested for alleged medical negligence. in both cases, autopsy reveals lesions to kidneys ...	2009	19269214
drug targets in infections with other emerging viruses: influenza viruses, metapneumovirus and hantaviruses.	among emergent and re-emergent viral infections, influenza, hemorrhagic fevers, including hantaviruses, constitute one of the major threats to human beings. advances in immunopathogenesis of these viral infections have improved initial supportive treatments and led to recognition and adoption of several useful antiviral agents. this review focuses on therapeutic and preventive aspects of these viral infections, and evaluates old and new promising agents are in the pipeline of pharmaceutical comp ...	2009	19275703
andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome.	hantavirus cardiopulmonary syndrome (hcps) is a highly pathogenic emerging disease (40% case fatality rate) caused by new world hantaviruses. hantavirus infections are transmitted to humans mainly by inhalation of virus-contaminated aerosol particles of rodent excreta and secretions. at present, there are no antiviral drugs or immunotherapeutic agents available for the treatment of hantaviral infection, and the survival rates for infected patients hinge largely on early virus recognition and hos ...	2009	19279096
platelet glycoprotein iib/iiia (hpa-1 and hpa-3) polymorphisms in patients with hemorrhagic fever with renal syndrome.	this study investigated the relationship between human platelet alloantigen (hpa) polymorphisms of glycoprotein iib/iiia and hemorrhagic fever with renal syndrome (hfrs). hpa-1 and hpa-3 genotyping was performed with allele-specific primer polymerase chain reaction in 104 patients with hfrs and 100 normal individuals as controls. the relationships between gene polymorphisms of hpas and hfrs and the disease severity were analyzed. the results indicated no significant difference in hpa-1 genotype ...	2009	19286442
bunyavirus n: eif4f surrogate and cap-guardian.	hantaviruses comprise a genus of the bunyavirus family of viruses. viruses of this family, along with the arenaviruses, and the orthomyxoviruses, including influenza, contain a negative sense, segmented rna genome. viral nucleocapsid proteins play a well-established role in the formation of intracellular and virion-associated nucleocapsids that harbor and shield viral genomic rna. however, recent observations indicate that hantavirus nucleocapsid protein (n) has additional unexpected biological ...	2009	19342890
bovine and human-derived passive immunization could help slow a future avian influenza pandemic.	an epidemic of human transmitted avian influenza could have casualties on a scale seen in the great spanish influenza pandemic of 1918. this paper proposes that should such occur before effective vaccines and antiviral drugs are available, the outbreak could be significantly slowed by consumption of raw milk produced by herds of pathogen-free lactating cows intranasally inoculated with heat-sterilized sputa pooled from avian influenza patients, supplemented by parenteral serum immune globulin fr ...	2009	18824305
nephropathia epidemica with a 6-week incubation period after occupational exposure to puumala hantavirus.	serological and molecular evidence showed that a german female student became infected by puumala hantavirus during mice trapping efforts in finland. the incubation period before exhibiting clinical signs of nephropathia epidemica was as long as 6 weeks. phylogenetic analysis of puuv nucleic acid sequences amplified from the patient demonstrate that she was infected by the puuv strain circulating at the place of her occupational exposure in finland.	2009	19058998
secretory expression and purification of recombinant escherichia coli heat-labile enterotoxin b subunit and its applications on intranasal vaccination of hantavirus.	in order to further study the b subunit of the escherichia coli heat-labile enterotoxin (ltb), we obtained the ltb gene from pathogenic e. coli, cloned it into the pet22b (+) prokaryotic expression vector, and expressed it as a fusion protein with his tag in e. coli bl21 (de3). the recombinant ltb was expressed and purified by ni2+ affinity chromatography. the biological activity of the purified recombinant ltb was assayed in a series of monosialotetrahexosylganglioside (gm1)-elisa experiments. ...	2009	19089660
construction and expression of a eukaryotic expression vector containing a fusion gene of the hantaan virus s gene and hsp70 gene.	hantavirus (hv) infection leads to a kind of severe systematic syndrome, hemorrhagic fever with renal syndrome (hfrs). heat shock proteins (hsps) can be used as adjuvants assisting soluble antigens to produce specific targets which can be attacked by cytotoxic t lymphocytes. for further research on hfrs vaccine, this study aimed to express hantaan virus nucleocapsid protein (htnv np)-hsp70 fusion protein in cos-7 cells. first, an htnv s gene encoding np was amplified by pcr with a mutated termin ...	2009	18810536
recent approaches in hantavirus vaccine development.	rodent-borne hantaviruses are associated with two main clinical disorders in humans: hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome. although hantavirus diseases can be life threatening and numerous research efforts are focused on the development of hantavirus prevention, no specific antiviral therapy is yet available and, at this time, no who-approved vaccine has gained widespread acceptance. this review will summarize the current knowledge and recent progress as ...	2009	19093774
predicting high risk for human hantavirus infections, sweden.	an increased risk for hemorrhagic fever with renal syndrome caused by puumala hantavirus was forecast for sweden in 2007. the forecast was based on a predicted increase in the number of myodes glareolus rodents (reservoir hosts). despite raised awareness and preparedness, the number of human cases during july 2007-june 2008 was 1,483, a new high.	2009	19116065
role of the nss protein in the zoonotic capacity of orthobunyaviruses.	the family bunyaviridae contains over 350 named isolates, classified into five genera: orthobunyavirus, hantavirus, nairovirus, phlebovirus and tospovirus. the orthobunyavirus genus contains some 170 isolates that are mainly transmitted by mosquitoes and are responsible for a range of disease syndromes in humans including self-limiting febrile illness, encephalitis and haemorrhagic fever. the viruses have a tripartite, negative-sense rna genome. analyses of viruses in four serogroups (bunyamwera ...	2009	18771514
hantavirus evolution in relation to its rodent and insectivore hosts: no evidence for codivergence.	hantaviruses are considered one of the best examples of a long-term association between rna viruses and their hosts. based on the appearance of strong host specificity, it has been suggested that hantaviruses cospeciated with the rodents and insectivores they infect since these mammals last shared a common ancestor, approximately 100 million years ago. we tested this hypothesis of host-virus codivergence in two ways: 1) we used cophylogenetic reconciliation analysis to assess the fit of the viru ...	2009	18922760
more than half of the patients with acute puumala hantavirus infection have abnormal cardiac findings.	this study was conducted to determine the frequency, severity and outcome of cardiac findings in patients with acute puumala hantavirus-induced nephropathia epidemica (ne). 70 consecutive, hospital-treated patients with serologically confirmed ne were prospectively examined using serial electrocardiograms (ecg), plasma troponin i, tumour necrosis factor alpha (tnf-alpha), interleukin-6 (il-6), and echocardiography (echo). examinations were repeated after 3 and 12 months. ecg changes were observe ...	2009	18932105
relating increasing hantavirus incidences to the changing climate: the mast connection.	nephropathia epidemica (ne), an emerging rodent-borne viral disease, has become the most important cause of infectious acute renal failure in belgium, with sharp increases in incidence occurring for more than a decade. bank voles are the rodent reservoir of the responsible hantavirus and are known to display cyclic population peaks. we tried to relate these peaks to the cyclic ne outbreaks observed since 1993. our hypothesis was that the ecological causal connection was the staple food source fo ...	2009	19149870
one for all? a viral protein supplants the mrna cap-binding complex.		2009	19129761
acute febrile illness caused by hantavirus: serological and molecular evidence from india.	study of hantavirus infections in india is in its early stages. as early symptoms of hantavirus disease can be non-specific and the diagnosis confirmed only by laboratory testing, use of appropriate diagnostic tools is important. to improve the diagnosis of hantavirus infections in india, commercial elisa systems followed by indirect immunofluorescence assays were used to detect anti-hantavirus igm and igg in samples from patients with acute febrile illness. of 347 patients tested, 5.2% showed s ...	2009	19237179
hantavirus infections in europe: from virus carriers to a major public-health problem.	in europe, hantavirus disease or hemorrhagic fever with renal syndrome is an endemic zoonosis that affects tens of thousands of individuals each year. the causative agents are viruses of the genus hantavirus, family bunyaviridae, rodents and insectivores act as carriers. in all european countries there is a seroprevalence for hantaviruses in the general population but not all countries report cases. here, we give an overview of the hantavirus situation in europe.	2009	19254169
[rodents and rodent associated disease vectors: the network of "rodent carrying pathogens" introduces itself].		2009	19263029
seroepidemiological study in a puumala virus outbreak area in south-east germany.	puumala virus (puuv) is the cause of the majority of haemorrhagic fever with renal syndrome cases in germany. in 2004, a nephropathia epidemica outbreak was recorded in lower bavaria, south-east germany. for a seroepidemiological study in this region including the resident population at four locations (n = 178) and soldiers from one location (n = 208) indirect immunoglobulin m (igm) and immunoglobulin g (igg) enzyme-linked immunosorbent assays (elisas) and immunoblot tests based on a yeast-expre ...	2009	19148676
healthcare-associated atypical pneumonia.	atypical pneumonia was first described in 1938, and over time, mycoplasma, legionella, and chlamydophila were the agents commonly linked with community-associated atypical pneumonia. however, as technology has improved, so has our understanding of this clinical entity. it is now known that there are many agents linked with atypical pneumonia in the community, and many of these agents are also major causes of healthcare-associated pneumonia. this article discusses the history, epidemiology, and p ...	2009	19199189
survey for antibody to hantaviruses in tamaulipas, mexico.	wild rodents (n=248) were trapped in two ecologically distinct sites at el cielo biosphere reserve in the state of tamaulipas, mexico, during the summer of 2003. samples from 199 individuals were tested for hantavirus antibodies by an indirect enzyme-linked immunosorbent assay (elisa). hantavirus antibodies to recombinant sin nombre virus nucleocapsid protein were found in seven rodents (3.5%) of a single species, peromyscus levipes. antibody-positive rodents were found only in the cloud forest ...	2009	19204351
the hantavirus glycoprotein g1 tail contains dual cchc-type classical zinc fingers.	hantaviruses are distributed worldwide and can cause a hemorrhagic fever or a cardiopulmonary syndrome in humans. mature virions consist of rna genome, nucleocapsid protein, rna polymerase, and two transmembrane glycoproteins, g1 and g2. the ectodomain of g1 is surface-exposed; however, it has a 142-residue c-terminal cytoplasmic tail that plays important roles in viral assembly and host-pathogen interaction. here we show by nmr, circular dichroism spectroscopy, and mutagenesis that a highly con ...	2009	19179334
potential renal sequelae in survivors of hantavirus cardiopulmonary syndrome.	although other hantaviruses are associated with renal manifestations, hantavirus cardiopulmonary syndrome (hcps) has not been associated with such sequelae. the hcps survivors were prospectively evaluated for renal complications. subjects underwent yearly evaluation, laboratory studies, and 24-hour urine collection. thirty subjects were evaluated after recovery from hcps with the first follow-up at a median of 7.4 months after discharge. subjects were a wide age range (18-51) but had an equal ge ...	2009	19190227
[anterior pituitary lobe atrophy as late complication of hemorrhagic fever with renal syndrome].	hemorrhagic fever with renal syndrome (hfrs) is acute infective multisystemic disease followed by febrility, hemorrhages and acute renal insufficiency. bleeding in the anterior pituitary lobe leading to tissue necrosis occurs in acute stage of severe clinical forms of hfrs, while atrophy of the anterior pituitary lobe with diminution of the gland function occurs after recovery stage.	2009	19281130
a novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses.	during cell culture isolation experiments to recover dobrava hantavirus from a suspension of liver from a striped field mouse (apodemus agrarius), an unknown virus was coisolated. atypically for hantaviruses, it had extensive cytopathic effects. using a random pcr approach, it was identified as a novel murine adenovirus, madv-3 (for madv type 3). a plaque-purified virus clone was prepared and further characterized. the complete genome sequence of madv-3 was determined to be 30,570 bp in length. ...	2009	19297486
hantavirus pulmonary syndrome, central plateau, southeastern, and southern brazil.	hantavirus pulmonary syndrome (hps) is an increasing health problem in brazil because of encroachment of sprawling urban, agricultural, and cattle-raising areas into habitats of subfamily sigmodontinae rodents, which serve as hantavirus reservoirs. from 1993 through june 2007, a total of 884 cases of hps were reported in brazil (case-fatality rate 39%). to better understand this emerging disease, we collected 89 human serum samples and 68 rodent lung samples containing antibodies to hantavirus f ...	2009	19331732
hantaan virus nucleocapsid protein binds to importin alpha proteins and inhibits tumor necrosis factor alpha-induced activation of nuclear factor kappa b.	hantaviruses such as hantaan virus (htnv) and andes virus cause two human diseases, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, respectively. for both, disease pathogenesis is thought to be immunologically mediated and there have been numerous reports of patients with elevated levels of proinflammatory and inflammatory cytokines, including tumor necrosis factor alpha (tnf-alpha), in their sera. multiple viruses have developed evasion strategies to circumvent the host ...	2009	19019947
[rare case of crimean haemorrhagic fever in a pregnant woman].	a rare case of crimean haemorrhagic fever in a pregnant woman during the sixth month of gestation is described. the typical clinical course of the disease is present with positive anamnesis and visible locus from tick bite. after several days, fever and local lymphadenitis appear, followed by severe toxicoinfectious and haemorrhagic syndromes. the disease is proved virologically and serologically. the outcome is favourable for the pregnant woman. she is discharged home after 16-day hospital trea ...	2009	20198776
[construction of a recombinant adenovirus carrying the hantaan virus glycoprotein g2 gene and its expression in hela cells].	we constructed the recombinant adenovirus expressing the glycoprotein g2 of hantaan virus. firstly we obtained coding gene fragment of g2 by pcr, and subsequently inserted the gene of interest into the adenoviral pshuttle vector pad5-cmv. then we co-transfected the recombinant pshuttle vector and adenovirus skeleton plasmid into hek293 cells by calcium phosphate precipitation method. after the recombinant adenovirus were packaged and amplified in hek293 cells, we observed the expression of repor ...	2009	20112705
hantavirus regulation of endothelial cell functions.	hantaviruses cause two vascular permeability-based diseases and primarily infect endothelial cells which form the primary fluid barrier of the vasculature. since hantavirus infections are not lytic, the mechanisms by which hantaviruses cause haemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps) are indeterminate. hps is associated with acute pulmonary oedema and hfrs with moderate haemorrhage and renal sequelae, perhaps reflecting the location of vast microvascular ...	2009	19967132
epidemiological study of hantavirus infection in the samara region of european russia.	european russia is a highly endemic area of hemorrhagic fever with renal syndrome (hfrs), a rodent-borne zoonotic disease, caused by hantaviruses. in total, 145 small mammals of four species (myodes glareolus, apodemus flavicollis, a. agrarius, and a. uralensis) were trapped in the samara region of european russia in august 2005 and examined for the presence of hantavirus (hv). anti-hv antibodies were found in six of 68 (8.8%) m. glareolus and in one of 19 (5.3%) a. flavicollis by indirect immun ...	2009	20046023
hantavirus pulmonary syndrome in five pediatric patients - four states, 2009.	hantavirus pulmonary syndrome (hps) is a reportable infectious disease with a high case-fatality rate, transmitted to humans by exposure to rodents. each year, 20--40 cases of hps occur in the united states; cases in persons aged <17 years make up fewer than 7% of those cases, and cases in children aged <10 years are exceptionally rare. cdc received reports of five pediatric cases of hps occurring during may 16--november 25, 2009, among children aged 6--14 years from arizona, california, colorad ...	2009	20032925
perspectives on scrub typhus, tick-borne pathogens, and hantavirus in the republic of korea.		2009	20088033
[from mice to men: the threat of hantavirus infections].	hantaviruses are naturally hosted by small mammals. virus transmission to humans can result in the development of hemorrhagic fever with renal syndrome (asia, europe) or hantavirus cardiopulmonary syndrome (the americas). certain hantaviruses can cause life-treatening infections with case fatality indices up to about 50%. different virus types have evolved which use different species of small mammals (mainly but not exclusively rodent species) as natural reservoirs. it is still unknown why these ...	2009	19999378
molecular epidemiological and serological studies of hantavirus infection in northern vietnam.	the distribution of anti-hantavirus antibodies in humans and rodents in northern vietnam was examined. in total, 837 serum samples from healthy humans (617) and patients with fever (220), living in six different areas were screened for igg antibodies against hantaan or seoul virus (seov) by elisa, ifa, and western blot analysis. antibody-positive sera were identified in 7/617 (1.1%) healthy donors, 5/150 port workers in the port of hai phong, and 2/185 residents of ha nam province. in comparison ...	2009	19887743
hsp70 induced by hantavirus infection interacts with viral nucleocapsid protein and its overexpression suppresses virus infection in vero e6 cells.	hantavirus (htv) infection is known to induce innate cellular response, a more specified cellular response in the host cells. however, whether it stimulates synthesis of stress proteins, particularly associations of viral proteins, is entirely unknown. the primary focus of this research is using vero e6 cells infected with hantaan 76-118 (htnv) as an in vitro infection model to examine the individual contribution of htv infection to heat shock response. this study shows that htnv infection rapid ...	2009	19956449
sympatry of 2 hantavirus strains, paraguay, 2003-2007.	to explore geographic and host-taxonomic patterns of hantaviruses in paraguay, we established sampling sites in the mbaracayu biosphere reserve. we detected jabora virus and itapua37/juquitiba-related virus in locations approximately 20 m apart in different years, which suggested sympatry of 2 distinct hantaviruses.	2009	19961679
simultaneous detection of cdc category "a" dna and rna bioterrorism agents by use of multiplex pcr & rt-pcr enzyme hybridization assays.	assays to simultaneously detect multiple potential agents of bioterrorism are limited. two multiplex pcr and rt-pcr enzyme hybridization assays (mpcr-eha, mrt-pcr-eha) were developed to simultaneously detect many of the cdc category "a" bioterrorism agents. the "bio t" dna assay was developed to detect: variola major (vm), bacillus anthracis (ba), yersinia pestis (yp), francisella tularensis (ft) and varicella zoster virus (vzv). the "bio t" rna assay (mrt-pcr-eha) was developed to detect: ebola ...	2009	20224751
cross-sectional survey of hantavirus infection, brazil.	a cross-sectional serosurvey was conducted to assess the proportion of persons exposed to hantaviruses in a virus-endemic area of the state of minas gerais, brazil. findings of this study suggested the presence of > or =1 hantaviruses circulating in this region causing hantavirus pulmonary syndrome, mild disease, or asymptomatic infection.	2009	19961680
[thrombocytopenia and acute kidney injury].	a 31-year old man is addressed to the emergency department for fever, abdominal pain and vomiting. laboratory tests reveal an inflammatory syndrome, thrombocytopenia, acute kidney injury associated with major proteinuria (more than 3 g/24 h). evaluation will conclude to an acute interstitial nephritis and the serology is positive for hantavirus. this clinical case illustrates differential diagnosis of acute renal failure and reminds the theory of hemorrhagic fever with renal syndrome : usual cli ...	2009	19998799