Publications

dna vaccination with hantavirus m segment elicits neutralizing antibodies and protects against seoul virus infection.	seoul virus (seov) is one of four known hantaviruses causing hemorrhagic fever with renal syndrome (hfrs). candidate naked dna vaccines for hfrs were constructed by subcloning cdna representing the medium (m; encoding the g1 and g2 glycoproteins) or small (s; encoding the nucleocapsid protein) genome segment of seov into the dna expression vector pwrg7077. we vaccinated balb/c mice with three doses of the m or s dna vaccine at 4-week intervals by either gene gun inoculation of the epidermis or n ...	1999	10069952
hantavirus pulmonary syndrome.	we present the first case of hantavirus pulmonary syndrome in the dallas/fort worth area. the patient was a 50-year-old previously healthy white man, who had adult respiratory distress syndrome (ards) and hypotensive shock after 1 week of nonspecific "viral" symptoms. despite supportive care, the patient died within several hours of presentation. this case illustrates several of the classic hallmarks of hantavirus infection such as hemoconcentration, thrombocytopenia, ards, and shock.	1999	10071675
spectrum of hantavirus infection: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome.	hantaviruses chronically infect rodents without apparent disease, but when they are spread by aerosolized excreta to humans, two major clinical syndromes result: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). both diseases appear to be immunopathologic, and inflammatory mediators are important in causing the clinical manifestations. in hps, t cells act on heavily infected pulmonary endothelium, and it is suspected that gamma interferon and tumor necrosis fa ...	1999	10073292
isolation and characterization of dobrava hantavirus carried by the striped field mouse (apodemus agrarius) in estonia.	dobrava hantavirus (dob) was isolated from the striped field mouse (apodemus agrarius) trapped on saaremaa island, estonia, and its genetic and antigenic characteristics were subsequently analysed. phylogenetic analysis showed that the estonian dob strain, together with several wild strains carried by apodemus agrarius, forms a well-supported lineage within the dob clade. the topography of the trees calculated for the s, m and l nucleotide sequences of the estonian dob suggests a similar evoluti ...	1999	10073697
emerging viral infections.		1999	10079847
[acute hantavirus infection caused by a genetically newly identified viral strain. severe and complicated course of hemorrhagic fever with renal syndrome (nephropathia epidemica)].	a 49-year-old patient, a hobby hunter, fell ill acutely with joint and limb pain, abdominal pain, nausea and subfebrile temperatures. at hospitalization, the patient was in bad general condition, showing ascites and lid edema, and acute renal failure was diagnosed.	1999	10081288
genetic diversity and distribution of peromyscus-borne hantaviruses in north america.	the 1993 outbreak of hantavirus pulmonary syndrome (hps) in the southwestern united states was associated with sin nombre virus, a rodent-borne hantavirus; the virus' primary reservoir is the deer mouse (peromyscus maniculatus). hantavirus-infected rodents were identified in various regions of north america. an extensive nucleotide sequence database of an 139 bp fragment amplified from virus m genomic segments was generated. phylogenetic analysis confirmed that snv-like hantaviruses are widely d ...	1999	10081674
climatic and environmental patterns associated with hantavirus pulmonary syndrome, four corners region, united states.	to investigate climatic, spatial, temporal, and environmental patterns associated with hantavirus pulmonary syndrome (hps) cases in the four corners region, we collected exposure site data for hps cases that occurred in 1993 to 1995. cases clustered seasonally and temporally by biome type and geographic location, and exposure sites were most often found in pinyon-juniper woodlands, grasslands, and great basin desert scrub lands, at elevations of 1,800 m to 2,500 m. environmental factors (e.g., t ...	1999	10081675
long-term studies of hantavirus reservoir populations in the southwestern united states: rationale, potential, and methods.	hantaviruses are rodent-borne zoonotic agents that cause hemorrhagic fever with renal syndrome in asia and europe and hantavirus pulmonary syndrome (hps) in north and south america. the epidemiology of human diseases caused by these viruses is tied to the ecology of the rodent hosts, and effective control and prevention relies on a through understanding of host ecology. after the 1993 hps outbreak in the southwestern united states, the centers for disease control and prevention initiated long-te ...	1999	10081676
long-term hantavirus persistence in rodent populations in central arizona.	for 35 months, we monitored hantavirus activity in rodent populations in central arizona. the most frequently captured hantavirus antibody-positive rodents were peromyscus boylii and p. truei. antibody-positive p. boylii were more frequently male (84%), older, and heavier, and they survived longer on trapping web sites than antibody-negative mice. the number of antibody-positive p. boylii was greater during high population densities than during low densities, while antibody prevalence was greate ...	1999	10081677
a longitudinal study of sin nombre virus prevalence in rodents, southeastern arizona.	we determined the prevalence of sin nombre virus antibodies in small mammals in southeastern arizona. of 1,234 rodents (from 13 species) captured each month from may through december 1995, only mice in the genus peromyscus were seropositive. antibody prevalence was 14.3% in 21 white-footed mice (p. leucopus), 13.3% in 98 brush mice (p. boylii), 0.8% in 118 cactus mice (p. eremicus), and 0% in 2 deer mice (p. maniculatus). most antibody-positive mice were adult male peromyscus captured close to o ...	1999	10081678
statistical sensitivity for detection of spatial and temporal patterns in rodent population densities.	a long-term monitoring program begun 1 year after the epidemic of hantavirus pulmonary syndrome in the u.s. southwest tracked rodent density changes through time and among sites and related these changes to hantavirus infection rates in various small-mammal reservoir species and human disease outbreaks. we assessed the statistical sensitivity of the program's field design and tested for potential biases in population estimates due to unintended deaths of rodents. analyzing data from two sites in ...	1999	10081679
natural history of sin nombre virus in western colorado.	a mark-recapture longitudinal study of immunoglobulin g (igg) antibody to sin nombre virus (snv) in rodent populations in western colorado (1994-results summarized to october 1997) indicates the presence of snv or a closely related hantavirus at two sites. most rodents (principally deer mice, peromyscus maniculatus, and pinyon mice, p. truei) did not persist on the trapping webs much beyond 1 month after first capture. some persisted more than 1 year, which suggests that even a few infected deer ...	1999	10081680
long-term studies of hantavirus reservoir populations in the southwestern united states: a synthesis.	a series of intensive, longitudinal, mark-recapture studies of hantavirus infection dynamics in reservoir populations in the southwestern united states indicates consistent patterns as well as important differences among sites and host-virus associations. all studies found a higher prevalence of infection in older (particularly male) mice; one study associated wounds with seropositivity. these findings are consistent with horizontal transmission and transmission through fighting between adult ma ...	1999	10081681
a midcourse assessment of hantavirus pulmonary syndrome.		1999	10081689
navigational instinct: a reason not to live trap deer mice in residences.		1999	10081690
genetic diversities of hantaviruses among rodents in hokkaido, japan and far east russia.	seroepizootiologic surveys among wild rodents were carried out in japan and far east russia in 1995 and 1996. seropositive animals were only identified in clethrionomys rufocanus (23/134) in hokkaido, japan. on the other hand, seropositives were identified in c. rufocanus (1/8), apodemus agrarius (2/66), apodemus spp. (2/26) and microtus fortis (3/22) in vladivostok, far east russia. total rna was isolated from lungs of seropositive animals and the s genome segments were amplified by pcr, cloned ...	1999	10082393
[hantavirus infection].		1999	10088345
hantavirus-specific igg, igm, and iga in acute and chronic renal disease versus congenital renal disease in the united states.	in a blinded fashion, 165 serum samples from patients with biopsy-characterized acute and chronic renal diseases (acrds), 34 serum samples from patients with congenital renal diseases (crds), and 100 serum samples from healthy adults were assayed for immunoglobulin g (igg), igm, and iga antibodies to hantaan and puumala viruses by enzyme immunoassay (eia). twenty-six percent (44 of 165) of acrds, 3% (1 of 34) of crds, and none (0 of 100) of the healthy serum samples were positive for hantavirus- ...	1999	10196017
cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins.	hantaviruses replicate primarily in the vascular endothelium and cause two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). in this report, we demonstrate that the cellular entry of hfrs-associated hantaviruses is facilitated by specific integrins expressed on platelets, endothelial cells, and macrophages. infection of human umbilical vein endothelial cells and vero e6 cells by the hfrs-causing hantaviruses hantaan (htn), seoul (seo), and puum ...	1999	10196290
hbv core particles allow the insertion and surface exposure of the entire potentially protective region of puumala hantavirus nucleocapsid protein.	core particles of the hepatitis b virus (hbv) potentiate the immune response against foreign epitopes presented on their surface. potential insertion sites in the monomeric subunit of the hbv core protein were previously identified at the n- and c-terminus and in the immunodominant c/e1 region. in a c-terminally truncated core protein these sites were used to introduce the entire 120 amino acid (aa)-long potentially immunoprotective region of the hantavirus (serotype puumala) nucleocapsid protei ...	1999	10223335
polymerase chain reaction detection of puumala virus rna in formaldehyde-fixed biopsy material.	infections with hantaviruses, mainly clethrionomys-derived puumala viruses, are known causes of acute renal failure [hemorrhagic fever with renal syndrome (hfrs)] in western europe. laboratory diagnosis is primarily based on serology. at the time of clinical symptoms, viral rna can hardly be detected in the blood or urine, indicating that polymerase chain reaction (pcr) is of little diagnostic value for these infections. biopsy material is usually formaldehyde-fixed and, thus, regarded as poor q ...	1999	10231472
relationships of deer mouse movement, vegetative structure, and prevalence of infection with sin nombre virus.	the effects of vegetative structure on movement of deer mice (peromyscus maniculatus) were examined in two distinct vegetation associations, one near hesperus and the other near molina in western colorado (usa) from june-october 1994 to october 1998. we monitored movement by live-trapping small mammals within gambel's oak/mixed-grass (hesperus) and sage brush/juniper (molina) vegetation types. vegetative structure differed between the sites with molina having more cover provided by shrubs and he ...	1999	10231758
emerging infectious diseases and pathogens.	emerging infectious diseases are caused by old, new, and mutant microorganisms. emergence of these pathogens can be attributed to changes in the characteristics and risk factors of patients, the widespread use of antibiotics, changes in the environment, the role of xenotransplantation, and international travel. in the united states, the incidences of c. difficile, cyclosporiasis, enterohemorrhagic e. coli gastroenteritis, hantavirus, hepatitis c virus infection, and lyme disease have increased s ...	1999	10318733
low prevalence of antibodies against the zoonotic agents brucella abortus, leptospira spp., streptococcus suis serotype ii, hantavirus, and lymphocytic choriomeningitis virus among veterinarians and pig farmers in the southern part of the netherlands.	serum samples from 102 veterinarians and 191 pig farmers from the southern part of the netherlands were investigated for antibodies against brucella abortus, leptospira spp, streptococcus suis serotype ii, hantavirus (hv), and lymphocytic choriomeningitis virus (lcmv). all samples were collected in 1993 and stored until this study was performed. the prevalence of antibodies against b.abortus in veterinarians (4.5%) was significantly higher (p = 0.01) than in pig farmers (0%). none of the veterin ...	1999	10321013
update on emerging infections from the centers for disease control and prevention. hantavirus pulmonary syndrome--colorado and new mexico, 1998.		1999	9867901
hantaviruses: an overview and radiographic correlation.	this article describes hantaviruses and the infections they cause. routes of transmission, disease phases and radiographic manifestations are discussed, along with recommendations for prevention and the radiologic technologist's role in prompt diagnosis.	1999	10101753
hantavirus nephropathy in a child.		1999	10328509
chronic renal dysfunction after hantavirus infection.		1999	10328510
genetic evidence of dobrava virus in apodemus agrarius in hungary.	using nested polymerase chain reaction, we sequenced dobrava virus (dob) from the rodent apodemus agrarius in hungary. the samples we isolated group with dob samples previously isolated from a. flavicollis. this grouping may indicate host switching.	1999	10341190
[new aspects of the pathogenesis of hantavirus infections. a systemic disease with general vascular changes].		1999	10354671
[the pathogenicity of hfrs patient's soft palate mucosa to suckling mice].	to explore the pathogenic mechanism of hemorrhagic fever with renal syndrome(hfrs).	1999	12759963
epidemiological progresses of hemorrhagic fever with renal syndrome in china.	to summarize the major achievements of chinese work on the epidemiology and prevention of hemorrhagic fever with renal syndrome (hfrs) in recent years, and to give a general review on the present situation of hfrs in china.	1999	11593522
[baculovirus: an example of an insect virus of use to humans].	baculovirus is a viral pathogen of insects in general and lepidoptera in particular. the genome of this large virus consists of a circular, infectious bicatenary dna molecule. at the end of its replication cycle in insects, baculovirus produces a large quantity of at least two proteins, i.e., polyedrine and polypeptide p10. these proteins are essential for transmission of the virus in nature, but are not necessary in cell cultures. using molecular recombinant techniques, one or both of the genes ...	1999	10901857
[topics of emerging, re-emerging infectious diseases].		1999	10866500
[an epidemiological analysis of hemorrhagic fever with renal syndrome morbidity in the republic of bashkortostan in 1997].	the outbreak of hemorrhagic fever with renal syndrome (hfrs) in the republic of bashkortostan, resulting in 10,057 registered cases of the disease (287 cases per 100,000 of the population), was analyzed. hfrs cases among the population were registered in 52 out of 54 regions of bashkortostan. 31% of the total number of patients were the inhabitants of rural regions (170 cases per 100,000) and 69% were urban dwellers (295 cases per 100,000), mainly in ufa (512 cases per 100,000). hfrs morbidity a ...	1999	10876849
[hantavirus in chile: review and cases analysis since 1975].	since the identification of the first case of hantavirus infection in chile in 1995, a great advance in the knowledge of the disease natural history has occurred. there is evidence to suppose that the infection by andes virus in suburban areas of argentina and chile, whose natural reservoir is oligoryzomys longicaudatus, has been present but unidentified for a long time. we describe a serologically proven case occurred in 1975. the clinical presentation of the disease slightly differs from that ...	1999	10835761
intravenous ribavirin for hantavirus pulmonary syndrome: safety and tolerance during 1 year of open-label experience. ribavirin study group.	intravenous ribavirin was provided non-selectively for investigational open-label use among persons with suspected hantavirus pulmonary syndrome (hps) in the united states between 4 june 1993 and 1 september 1994. therapy was initiated prior to laboratory confirmation of hantavirus infection because most deaths from hps occur within 48 h of hospitalization. thirty patients with confirmed hps, 105 patients without hps and 5 patients without adequate diagnostic testing for hps were enrolled. this ...	1999	10723500
hantavirus dobrava infection with pulmonary manifestation.	dobrava virus infection was diagnosed serologically by enzyme-linked immunosorbent and immunofluorescence assays. to determine which hantavirus serotype was involved, sera were analyzed by a focus reduction neutralization test. the clinical data indicated that only pulmonary manifestation was present. our data support the presence of dobrava virus infection outside the balkan region. in conclusion, a previously healthy adult with unexplained pulmonary perfusion failure should be investigated for ...	1999	10691093
hantavirus pulmonary syndrome: an alert to latin american countries.	approximately 400 cases of hantavirus pulmonary syndrome (hps) have been reported on the north american continent since the first epidemic in 1993, in new mexico, usa. in brazil, 12 cases were confirmed before 1998. infection results from inhalation of rodent blood, saliva or waste. clinical findings of the disease are characterized by a nonspecific febrile illness, followed by rapid progression to pulmonary edema which may lead to a shock-like state or renal failure. mortality is high and emerg ...	1999	11084670
implications of the analogy between recombinant cytokine toxicities and manifestations of hantavirus infections.	the etiologic hantavirus of the 1993 emergence of an acute pulmonary failure syndrome in the area around northwestern new mexico was quickly recognized as related to the hantaan virus responsible for the outbreak of korean epidemic hemorrhagic fever (ehf) among un troops in 1951. discovery of the new disease which was named the hantavirus pulmonary syndrome (hps) and its causative agent the sine nombre virus (snv) inspired detailed comparisons between the two disorders. major damage to the epith ...	1998	10850356
anne boleyn, the sweating sickness, and the hantavirus: a review of an old disease with a modern interpretation.		1998	11619876
[studies on the experimental transmission of rattus-borne hantavirus by ornithonyssus bacoti].	to demonstrate the role of rat mite (ornithonyssus bacoti) in the transmission of rattus-borne hemorrhagic fever with renal syndrome (hfrs).	1998	12078291
were hantaviruses eventually responsible for the lost anasazi culture?		1998	10358738
assessment of person-to-person transmission of hantavirus pulmonary syndrome in a chilean hospital setting.	person-to-person transmission of andes hantavirus among healthcare workers was reported in argentina for the first time in 1996. to determine whether such transmission of the virus occurred during a 1997 outbreak of hantavirus pulmonary syndrome (hps) in southern chile due to andes virus, we conducted a serological and epidemiological study in the coyhaique regional hospital, where the majority of hps patients were admitted. workers in every department of the hospital were evaluated for immunogl ...	1998	9868619
the muskrat (ondatra zibethicus) as a new reservoir for puumala-like hantavirus strains in europe.	we have used an indirect immunofluorescent assay (ifa) and reverse transcription-pcr (rt-pcr) to screen the sera and tissues of muskrats (ondatra zibethica) caught in the northwest of brandenburg and in the northeast of saxony-anhalt, germany, for hantavirus infection. kidney and/or lung tissue from 6 (3.1%, ci = 1.1-6.5%) out of 197 muskrats were found to be positive for genomic sequences of hantavirus by rt-pcr. we could also demonstrate that 14 (5%, ci = 2.9-8.7%) out of 266 muskrat's sera av ...	1998	9870582
new world hantaviruses.	since the initial description in 1993 of hantavirus pulmonary syndrome and its novel aetiological agent, sin nombre virus, our knowledge of the epidemiology of new world hantaviruses has continued to evolve. after the identifying outbreak in the southwestern us, four hantaviruses have been identified in north america with specific rodent hosts and associated with a number of sporadic cases. this stability of case recognition in north america is in contrast to the multiple outbreaks and endemic c ...	1998	10326292
maternal transfer of hantavirus antibodies in rats.		1998	10090051
[hantavirus and pulmonary pathology].	the hantavirus pulmonary syndrome recognized in the united states in 1993 quickly brought the formerly little known virus into the limelight. contrary to what has been written almost everywhere, this is not a "new" virus causing new "emerging" disease. hantaviruses have been harbored in their natural hosts, three subfamilies of murine rodents, for millions of years. the phylogenetic classifications of these viruses follows that of their hosts, proving close adaptation and contradicting short-ter ...	1998	10100354
first case of infection with hantavirus dobrava in germany.		1998	10052557
high-producer allele of tumour necrosis factor-alpha is part of the susceptibility mhc haplotype in severe puumala virus-induced nephropathia epidemica.	the biallelic polymorphisms in the tumour necrosis factor-alpha (tnf-alpha) gene promoter region were studied in patients with nephropathia epidemica (ne). the rarer tnf2 allele, associated with a high tnf-alpha producer phenotype, was more frequently found in hospitalized ne patients (42%) than in healthy controls (15%; p=0.0064). the putative role of tnf2 as an independent risk factor for ne is discussed.	1998	10066063
development of humoral cross-reactivity to the nucleocapsid protein of heterologous hantaviruses in nephropathia epidemica.	a hantavirus infection is followed by a prominent antibody response to the viral nucleocapsid protein. antibodies from patients infected with one hantavirus cross-react to varying degrees with the nucleocapsid protein of other viruses of the genus. we studied the cross-reactivity in serially obtained blood samples from 17 patients with nephropathia epidemica, a european form of hemorrhagic fever with renal syndrome caused by puumala virus. recombinant truncated nucleocapsid protein (aa 1-117) of ...	1998	9879922
[hantaviruses--a global problem].		1998	9885644
[diseases caused by hantaviruses].	hantaviruses are rna viruses that belong to the genus hantavirus, family bunyaviridae. the main reservoir of hantaviruses are rodents. humans become infected by inhaling particles of dust contaminated with faeces of rodents, that are found in europe and asia hantaviruses named: hantaan, seoul and dobrava-belgrade causing haemorrhagic fever with renal syndrome (hfrs) are prevalent. typical clinical picture of hfrs consists of renal insufficiency (primarily anuria and then polyuria with disturbanc ...	1998	9919918
stable expression of nucleocapsid proteins of puumala and hantaan virus in mammalian cells.	the development of an in vitro-system for the stable expression and the analysis of native hantavirus proteins using hantaviral cdna is of particular interest. as a first step the expression of the hantavirus nucleocapsid (n) proteins in mammalian cells was studied in more detail. the cdna of the s-rna segment of puumala virus strain cg-1820 and hantaan virus strain 76-118 was used for the construction of eucaryotic expression vectors that allow the generation and selection of mammalian cells ha ...	1998	9857992
ecologic studies of rodent reservoirs: their relevance for human health.	within the past few years, the number of "new" human diseases associated with small-mammal reservoirs has increased dramatically, stimulating renewed interest in reservoir ecology research. a consistent, integrative approach to such research allows direct comparisons between studies, contributes to the efficient use of resources and data, and increases investigator safety. we outline steps directed toward understanding vertebrate host ecology as it relates to human disease and illustrate the rel ...	1998	9866729
an outbreak of hantavirus pulmonary syndrome, chile, 1997.	an outbreak of 25 cases of andes virus-associated hantavirus pulmonary syndrome (hps) was recognized in southern chile from july 1997 through january 1998. in addition to the hps patients, three persons with mild hantaviral disease and one person with asymptomatic acute infection were identified. epidemiologic studies suggested person-to-person transmission in two of three family clusters. ecologic studies showed very high densities of several species of sigmodontine rodents in the area.	1998	9866751
short report: decrease in seroprevalence of antibodies to hantavirus in rodents from 1993-1994 hantavirus pulmonary syndrome case sites.	rodent trapping was conducted at seven hantavirus pulmonary syndrome (hps) case sites from june 1993 to march 1994 during the hps outbreak in the southwestern united states. to determine if there were changes in the rodent population or the hantavirus seroprevalence in rodents since the hps outbreak, rodents were trapped at the same sites three years later using the same trapping protocol. the trap success decreased from the numbers trapped during the outbreak, however, the number of peromyscus, ...	1998	9660455
retrospective serological and genetic study of the distribution of hantaviruses in greece.	a retrospective serological and genetic study of hantaviruses responsible for hemorrhagic fever with renal syndrome (hfrs) in greece during the last 17 years is presented. fifty-one serum samples taken from 30 hfrs cases previously diagnosed by immunofluorescence assay were tested by elisa for igg (hantaan, dobrava, and puumala) and igm antibodies (hantaan and puumala). results were compatible with the majority of infections being related to hantaviruses carried by rodents of the subfamily murin ...	1998	9661842
acute exacerbation of autoimmune liver disease associated with hantaviral infection.	hantavirus is known to cause haemorrhagic fever with renal syndrome (hfrs). although liver dysfunction has been reported in hfrs, hepatic manifestations of hantaviral infection have not been well described. we describe a case of autoimmune cholangitis in which an exacerbation of hepatitis was associated with hantaviral infection. seroconversion of both igg- and igm-class antibodies to hantavirus was noted coincident with acute exacerbation of hepatitis, which was resolved promptly by treatment w ...	1998	9670364
distinguishing hantavirus pulmonary syndrome from acute respiratory distress syndrome by chest radiography: are there different radiographic manifestations of increased alveolar permeability?	hantavirus infection may cause diffuse air space disease, termed hantavirus pulmonary syndrome (hps). the authors sought to determine if chest radiographs could differentiate hps from typical acute respiratory distress syndrome (ards). the authors identified patients with either hps (n = 11) or acute ards (n = 32) and selected the earliest chest radiograph showing diffuse airspace disease, and a chest radiograph taken 24 to 48 hours previously. thoracic and general radiologists first viewed the ...	1998	9671418
adjuvant activity of muramyl dipeptide derivatives to enhance immunogenicity of a hantavirus-inactivated vaccine.	the adjuvant effect of two lipophilic derivatives of muramyl dipeptide (mdp), b30-mdp and mdp-lys(l18), on the ability of an inactivated vaccine of b-1 virus (b-1 vaccine) to induce immune response against hantavirus causing hemorrhagic fever with renal syndrome (hfrs) was examined. when mice were immunized subcutaneously (s.c.) twice at 2-week intervals with b-1 vaccine admixed with or without 100 micrograms mouse-1 of b30-mdp (b-1/b30-mdp) or mdp-lys(l18) [b-1/mdp-lys(l18)], mice immunized wit ...	1998	9607033
chimaeric hbv core particles carrying a defined segment of puumala hantavirus nucleocapsid protein evoke protective immunity in an animal model.	hantaviruses are rodent-born agents which are pathogenic in humans causing haemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome. to induce a protective immunity against a european hantavirus (puumala) we constructed chimaeric hepatitis b virus (hbv) core particles carrying defined fragments of the puumala virus nucleocapsid protein. after immunisation of bank voles, the natural host of puumala virus, with core particles possessing an insertion of the n-terminal part of puumala ...	1998	9607042
tissue distribution of hantavirus antigen in naturally infected humans and deer mice.	the sin nombre virus (snv) is the etiologic agent of hantavirus pulmonary syndrome in humans but does not cause disease in chronically infected deer mice (peromyscus maniculatus), the natural host. in this study, murine antiserum raised against recombinant snv nucleocapsid protein was utilized to localize viral antigen immunohistochemically in tissues from both humans (n = 20; 11 positive, 9 negative) and deer mice (n = 6; 4 positive, 2 negative). viral infection status was confirmed by western ...	1998	9607851
haemorrhagic fever with renal syndrome caused by puumala virus: evaluation of the risk for cataract formation.		1998	9614533
glomerular basement membrane antibodies in hantavirus disease: any pathogenetic implication?		1998	9617503
beta3 integrins mediate the cellular entry of hantaviruses that cause respiratory failure.	newly emerged hantaviruses replicate primarily in the pulmonary endothelium, cause acute platelet loss, and result in hantavirus pulmonary syndrome (hps). we now report that specific integrins expressed on platelets and endothelial cells permit the cellular entry of hps-associated hantaviruses. infection with hps-associated hantaviruses, ny-1 and sin nombre virus (snv), is inhibited by antibodies to beta3 integrins and by the beta3-integrin ligand, vitronectin. in contrast, infection with the no ...	1998	9618541
prevalence of antibodies specific to puumala virus among farmers in sweden.	the puumala virus is the causative agent of nephropathia epidemica, a european form of hemorrhagic fever with a renal syndrome. from its reservoir in bank voles, the virus is spread by airborne transmission to humans. occupational risks for the acquisition of nephropathia epidemica are not well defined. the prevalence of serum antibodies to puumala virus was determined for swedish farmers. from a comparison of the prevalence among farmers from various parts of the country, the assumption that pu ...	1998	9630057
hantavirus pulmonary syndrome--colorado and new mexico, 1998.	hantavirus pulmonary syndrome (hps) is a severe cardiopulmonary illness resulting in death in approximately 45% of cases. the most frequently recognized etiologic agent of hps in north america, sin nombre virus (snv), is transmitted to humans from its primary rodent reservoir, peromyscus maniculatus (deer mouse), by direct contact with infected rodents, rodent droppings, or nests or through inhalation of aerosolized virus particles from mouse urine and feces. sporadic cases occur throughout the ...	1998	9639364
surveillance of anti-hantavirus antibodies among certain high-risk groups in taiwan.	hemorrhagic fever with renal syndrome is a serious health concern in neighboring countries of taiwan, such as mainland china and korea. in taiwan, only two suspected cases were recorded before 1994. the first confirmed case was reported in 1995, but this proved to be imported. to study hantavirus infection in taiwan, we tested blood collected from garbage collectors, animal handlers, patients with febrile illness of unknown origin, and field rats, the host of hantavirus, for the presence of anti ...	1998	9481070
hantavirus pulmonary syndrome.		1998	9493883
viral hemorrhagic fevers and hantavirus infections in the americas.	several arenaviruses and hantaviruses have been isolated in the americas during the last 4 decades. these are rodent-borne viruses responsible for the south american hemorrhagic fevers (sahf) and hantavirus pulmonary syndrome (hps). although rare, sahf and hps are serious illnesses with high mortality rates. most viral isolates found in the americas represent new world lineages of their respective viral families. their presence in the western hemisphere is likely ancient, their relationship with ...	1998	9494832
emerging infectious diseases and travel medicine.	international movement of individuals, populations, and products is one of the major factors associated with the emergence and reemergence of infectious diseases as the pace of global travel and commerce increases rapidly. travel can be associated with disease emergence because (1) the disease arises in an area of heavy tourism, (2) tourists may be at heightened risk because of their activities, or (3) because they can act as vectors to transport the agent to new areas. examples of recently reco ...	1998	9494841
genetic diversity and epidemiology of hantaviruses in argentina.	phylogenetic analysis of a 292-nucleotide (nt) fragment of the hantavirus m genome segment from 36 rodent and 13 human samples from three known foci of hantavirus infection in argentina was conducted. a 1654-nt fragment of the m genome segment was analyzed for 1 representative of 7 genetically distinct hantavirus lineages identified. additionally, the nt sequence of the complete m genome segments of lechiguanas, oran, and hu39694 hantavirus genotypes was determined. nt sequence comparisons revea ...	1998	9498428
evaluation of puumala virus igg and igm enzyme immunoassays based on recombinant baculovirus-expressed nucleocapsid protein for early nephropathia epidemica diagnosis.	puumala virus (puu), a member of hantavirus genus, is the causative agent of nephropathia epidemica (ne), a milder form of hemorrhagic fever with renal syndrome (hfrs). rapid diagnosis is essential for clinical management of ne.	1998	9646005
pathogenesis of puumala and other hantavirus infections.	hantaviruses are rodent/insectivore-borne negative-stranded rna viruses which belong to the bunyaviridae family. they do not cause any symptomatic disease in their adult carrier rodents, but in humans they are aetiologic agents of haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps), both associated with a significant mortality. in cell culture hantaviruses do not cause cytopathic effects and the mechanisms of disease in man are not well understood. increased cap ...	1998	10398496
genetic analysis of the diversity and origin of hantaviruses in peromyscus leucopus mice in north america.	nucleotide sequences were determined for the complete m genome segments of two distinct hantavirus genetic lineages which were detected in hantavirus antibody- and pcr-positive white-footed mice (peromyscus leucopus) from indiana and oklahoma. phylogenetic analyses indicated that although divergent from each other, the virus lineages in indiana and oklahoma were monophyletic and formed a newly identified unique ancestral branch within the clade of sin nombre-like viruses found in peromyscus mice ...	1998	9420200
severe thrombocytopenia and response to corticosteroids in a case of nephropathia epidemica.	nine days after working in the woods, a previously healthy 32-year-old man fell seriously ill. his symptoms included high fever, chills, diffuse myalgia, severe headache, and back pain. on the fifth day of onset of symptoms, blood tests showed creatinine levels of 5.4 mg/dl accompanied by marked proteinuria. after admission to the hospital, a diagnosis of nephropathia epidemica (ne) caused by puumala virus was made using solid-phase enzyme-linked immunosorbent assay (elisa). the patient graduall ...	1998	9428461
hantavirus pulmonary syndrome in a chilean patient with recent travel in bolivia.	a case of hantavirus pulmonary syndrome (hps) was serologically confirmed in a critically ill patient in santiago, chile. the patient's clinical course had many similarities to that of other hps patients in north and south america but was complicated by acute severe renal failure. the patient's history included self-reported urban and probable rural rodent exposure during travel in bolivia. comparison of a viral sequence from an acute-phase serum sample with other known hantaviruses showed that ...	1998	9452401
bayou virus-associated hantavirus pulmonary syndrome in eastern texas: identification of the rice rat, oryzomys palustris, as reservoir host.	we describe the third known case of hantavirus pulmonary syndrome (hps) due to bayou virus, from jefferson county, texas. by using molecular epidemiologic methods, we show that rice rats (oryzomys palustris) are frequently infected with bayou virus and that viral rna sequences from hps patients are similar to those from nearby rice rats. bayou virus is associated with o. palustris; this rodent appears to be its predominant reservoir host.	1998	9452404
hantavirus infection in children in argentina.	clinical hantavirus infection was diagnosed in five argentine children ages 5 to 11 years by immunoglobulin m (igm)- capture enzyme-linked immunosorbent assay using sin nombre virus (snv) antigens. death in three of the children was associated with absence of detectable igg to snv antigens. an additional two cases in healthy children were studied: one, a breast-fed 15-month-old whose mother died of suspected hantavirus pulmonary syndrome (hps) 8 months previously, had hantavirus igg (> 1:6400); ...	1998	9454562
severe hantavirus pulmonary syndrome: a new indication for extracorporeal life support?		1998	9468156
successful treatment of adults with severe hantavirus pulmonary syndrome with extracorporeal membrane oxygenation.	to describe our experience with the use of extracorporeal membrane oxygenation (ecmo) as a rescue therapy in adult patients with severe cardiopulmonary failure from hantavirus pulmonary syndrome.	1998	9468181
small mammal survival and trapability in mark-recapture monitoring programs for hantavirus.	following the 1993 hantavirus pulmonary syndrome (hps) epidemic in the south-western united states, mammalogists and epidemiologists instituted long-term studies to monitor population density and prevalence of infection in rodents which constitute the reservoir for sin nombre virus (snv). in this study, field techniques used in sampling small mammals for snv infection were evaluated to determine if trapping and handling protocols were having significant effects on future trapability or mortality ...	1998	9476220
hantavirus pulmonary syndrome outbreak in argentina: molecular evidence for person-to-person transmission of andes virus.	an increase of hantavirus pulmonary syndrome (hps) cases around a southwestern argentina town and in persons living 1400 km away but in contact with those cases was detected during the spring of 1996. in order to evaluate person-to-person transmission we compared the homology of pcr-amplified viral sequences of 26 argentine and chilean cases. sixteen of them were epidemiologically linked cases and had the same sequence (epilink/96) in the s segment 3' noncoding region and in the m segment partia ...	1998	9499807
genetic reassortment among viruses causing hantavirus pulmonary syndrome.	in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ...	1998	9501041
urine-associated horizontal transmission of seoul virus among rats.	to understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with the ki-83-262 strain of seoul virus, s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ...	1998	9505963
a miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers.	in this paper, we describe a miniature analytical thermal cycling instrument (matci) to amplify and detect dna via the polymerase chain reaction in real-time. the matci is an integrated, miniaturized analytical system that uses silicon-based, high-efficiency reaction chambers with integrated heaters and simple, inexpensive electronics to precisely control the reaction temperatures. optical windows in the silicon and solid-state, diode-based detection components are employed to perform real-time ...	1998	9511467
hantavirus pulmonary syndrome: implications for critical care nurses.		1998	9515465
association of hla b27 with benign clinical course of nephropathia epidemica caused by puumala hantavirus.	both the severe course of nephropathia epidemica (ne) caused by puumala hantavirus, and the fast progression of human immunodeficiency virus (hiv) disease, are associated with the hla b8 drb1*0301 haplotype. as hla b27, on the contrary, is associated with the slow progression of hiv disease, we wanted to test whether the same is true for ne. only six (8%) ne patients, half the figure expected, had the hla b27 allele in 74 randomly selected hospital-treated patients. all six had a benign overall ...	1998	9519867
hantavirus infection.		1998	9520902
role of actin microfilaments in black creek canal virus morphogenesis.	we have investigated the involvement of cytoskeletal proteins in the morphogenesis of black creek canal virus (bccv), a new world hantavirus. immunofluorescent staining of bccv-infected cells revealed a filamentous pattern of virus antigen, the appearance of which was sensitive to treatment with cytochalasin d, an actin microfilament-depolymerizing drug. double immunofluorescence staining of bccv-infected vero cells with anti-bccv nucleocapsid (n) monoclonal antibody and phalloidin revealed a co ...	1998	9525606
neuropsychological impairments following hantavirus pulmonary syndrome.	recently an outbreak of acute respiratory infection associated with the hantavirus occurred in the southwestern united states. hantavirus pulmonary syndrome (hps) is a life threatening illness that carries with it a high mortality rate. patients with hps experience prolonged periods of hypoxemia requiring mechanical ventilation and treatment in intensive care units. we have recently seen 2 survivors of hps. a neuropsychological test battery was administered immediately following their acute hosp ...	1998	9529829
serologic survey for hantavirus infection in domestic animals and coyotes from new mexico and northeastern arizona.	to determine whether animals had serologic evidence of infection with sin nombre virus (snv).	1998	9540866
urine-associated horizontal transmission of seoul virus among rats.	to understand the mode of transmission of seoul type hantavirus in wistar rats, we examined the shedding of the virus and antibody production in infected rats. when 1-day-old rats were inoculated with ki-83-262 strain of seoul virus, the s segment of the viral genome was detected in lungs, clots, urine, saliva, submaxillary glands, rectums, and kidneys by nested reverse transcriptase pcr. on the other hand, when 8-week-old rats were infected with the virus, viral genome was detected only in the ...	1998	9541619
of mice and mostly men--hantavirus pulmonary syndrome.		1998	9559018
human recombinant puumala virus antibodies: cross-reaction with other hantaviruses and use in diagnostics.	a panel of seven human monoclonal fabs against puumala virus (puu) nucleocapsid protein (n) was obtained by panning an antibody phage-display library prepared from the spleen of a puu-immune individual. three antibodies reacted in immunoblotting and cross-reacted strongly with tula and sin nombre virus recombinant n proteins. these antibodies mapped to the amino terminus of the n protein. one puu glycoprotein 2 (g2)-specific fab obtained against a novel epitope (g2c) cross-reacted with khabarovs ...	1998	9568958
central nervous system and ophthalmic involvement in nephropathia epidemica (european type of haemorrhagic fever with renal syndrome).	central nervous system (cns) - related symptoms occur in haemorrhagic fever with renal syndrome (hfrs). to study the cns and ophthalmic involvement in nephropathia epidemica (ne), the european type of hfrs, we included 26 patients in a prospective study. most common cns-related symptoms were headache (96%), insomnia (83%), vertigo (79%), nausea (79%), and vomiting (71%). ophthalmic symptoms were reported by 82% of patients; 41% had photophobia and 50% had impaired vision. a transient loss of vis ...	1998	9570645
a survey of hantavirus antibody in small-mammal populations in selected united states national parks.	hantavirus activity in 39 national parks in the eastern and central united states was surveyed by testing 1,815 small mammals of 38 species for antibody reactive to sin nombre virus. antibody-positive rodents were found throughout the area sampled, and in most biotic communities. antibody was detected in 7% of 647 deer mice (peromyscus maniculatus), 2% of 590 white-footed mice (p. leucopus), 17% of 12 rice rats (oryzomys palustris), 3% of 31 cotton rats (sigmodon hispidus), and 33% of 18 western ...	1998	9574803
hantavirus infection: a possible cause of delayed sensorineural hearing loss.		1998	9591876