Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [bunyavirus--virus and host relationship: the coevolution between hantavirus and rodent]. | 2002 | 12227181 | |
| spatiotemporal patterns in the hantavirus infection. | we present a model of the infection of hantavirus in deer mouse, peromyscus maniculatus, based on biological observations of the system in the north american southwest. the results of the analysis shed light on relevant observations of the biological system, such as the sporadical disappearance of the infection, and the existence of foci or "refugia" that perform as reservoirs of the virus when environmental conditions are less than optimal. | 2002 | 12241389 |
| detection of hantavirus infection in hemolyzed mouse blood using alkaline phosphatase conjugate. | conventional methods such as elisa and culture require a long time for the determination of viral infections. fast and reliable instrumentation suitable for operation under field conditions that allows rapid detection of hantavirus in rodent populations in the wild, would be a substantial improvement over these methods. in response to this challenge, a prototype amperometric immunosensor based on highly dispersed immunoelectrode for rapid, sensitive and quantitative assay of hantavirus in mice b ... | 2002 | 12243909 |
| yeast-expressed puumala hantavirus nucleocapsid protein induces protection in a bank vole model. | hantaviruses are rodent-borne agents that cause severe human diseases. the coding sequences for the authentic and a his-tagged puumala hantavirus (puuv) nucleocapsid (n) protein were expressed in yeast (saccharomyces cerevisiae). n-specific monoclonal antibodies demonstrated native antigenicity of the two proteins. all bank voles vaccinated with the his-tagged n protein in freund's adjuvant (n=12) were defined as completely protected against subsequent virus challenge, based on the absence of vi ... | 2002 | 12297397 |
| wild-type puumala hantavirus infection induces cytokines, c-reactive protein, creatinine, and nitric oxide in cynomolgus macaques. | hantaviruses cause two severe human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). approximately 200,000 cases are reported annually, and there is to date no specific treatment available. a major obstacle in studying the medical aspects of hfrs and hps has been the lack of an adequate animal model. here we show that infection of cynomolgus macaques by wild-type puumala hantavirus resulted in typical signs of hfrs including lethargy, anorexia, prot ... | 2002 | 11739712 |
| first reported case of imported hantavirus pulmonary syndrome in europe. | 2002 | 11749764 | |
| radiographic findings in 20 patients with hantavirus pulmonary syndrome correlated with clinical outcome. | hantavirus is a rare rodent-borne pathogen responsible for the hantavirus pulmonary syndrome. the objective of this study was to review the clinical and radiographic findings of patients presenting with hantavirus pulmonary syndrome in northern alberta, canada. | 2002 | 11756112 |
| puumala virus pulmonary syndrome in a romanian immigrant. | hantaviruses belong to the bunyaviridae family, which is comprised of bunyavirus, phlebovirus, nairovirus, and hantavirus. euroasiatic hantaviruses belong to two distinct subfamilies: murinae (comprising hantaan, dobrava, and seoul viruses), which are responsible for hemorrhagic fever with renal syndrome (hfrs), and arvicolinae (comprising puumala virus), responsible for nephropathia epidemica (ne) and hfrs. on the contrary, the new world hantavirus belongs to the sigmodontinae subfamily and inc ... | 2002 | 12962589 |
| experimental evaluation of rodent exclusion methods to reduce hantavirus transmission to residents in a native american community in new mexico. | we conducted a pilot study to evaluate the efficacy of rodent proofing continuously occupied homes as a method for lowering the risk for hantavirus pulmonary syndrome (hps) among residents of a native american community in northwestern new mexico. rodent proofing of dwellings was paired with culturally appropriate health education. seventy homes were randomly assigned to treatment or control categories. treatment homes were rodent-proofed by sealing openings around foundations, doors, roofs, and ... | 2002 | 12653299 |
| zoonoses--an interview with james emory childs, sc.d. | 2002 | 12653306 | |
| genetic evidence for tula virus in microtus arvalis and microtus agrestis populations in croatia. | to determine the threat of hantavirus infection to u.s. forces, small mammals were sampled from training areas within croatia. of the 152 samples, 20 were positive for tula virus (tul), 12 common voles (microtus arvalis) and eight field voles (microtus agrestis). sequences from m. agrestis were found in five and sequences from m. arvalis were found in six of seven sequence groups. the high percentage of the same tul sequences in m. agrestis and m. arvalis suggests the co-occurrence of this virus ... | 2002 | 12656127 |
| distribution of puumala hantavirus in denmark: analysis of bank voles (clethrionomys glareolus) from fyn and jutland. | the majority of hantaviral infections in denmark since 1957 have occurred on the island of fyn. we have recently shown the presence of puumala hantavirus (puuv) in bank voles (clethrionomys glareolus) on fyn. to learn more about the distribution and prevalence of puuv in denmark, 310 small mammals, belonging to six rodent and two insectivore species, were trapped in four localities on fyn and three localities on the jutland peninsula (mainland denmark), where only a few cases of nephropathia epi ... | 2002 | 12656129 |
| rapid and sensitive handheld biosensor for detection of hantavirus antibodies in wild mouse blood samples under field conditions. | hantavirus cardiopulmonary syndrome (hcps) is an acute, life threatening viral illness that was first recognized in the spring of 1993 during an outbreak in the four corners region of the southwest united states. the etiologic agent, sin nombre (sn) virus, is a zoonotic infection that is enzootic in deer mice (peromyscus maniculatus). both human and rodent infections lead to specific antibody responses, and detection of such antibodies is the mainstay of diagnosis of infection in both species. m ... | 2002 | 18968779 |
| serological study of hantavirus in man in the autonomous community of madrid, spain. | data relating to hantavirus infection in spain are scarce and limited to rural areas. the aim of this work was to study the seroprevalence of hantavirus infection in the autonomous community of madrid (acm), a region containing both rural and urban populations in different ecological settings. sera from 3852 individuals (1849 male, 2003 female) were screened by indirect inmunofluorescence, with vero e6 cells infected with puumala, hantaan and seoul viruses as antigens. screen-positive results we ... | 2002 | 12435066 |
| hantavirus pulmonary syndrome in manitoba. | the first confirmed case of hantavirus pulmonary syndrome in manitoba was diagnosed in 1999. to define better the risk of exposure to hantaviruses in this area, the clinical features and epidemiological factors pertaining to this case were described, and a serological survey of rodents collected near the patient's residence was undertaken. small mammals were collected using live traps, were anesthetized via inhalation of isoflurane and were bled. human and mouse serologies were undertaken using ... | 2001 | 18159335 |
| [acute tubulointerstitial nephritis in children]. | acute tubulointerstitial nephritis (atin) is a rare renal disorder in children. patients usually present non-specific symptoms and signs so that the diagnosis of atin is often delayed. the disease may be infection- or drug-induced or it may occur without a known cause. early recognition and appropriated therapy usually lead to an excellent prognosis. the aim of the study was to describe clinical and laboratory findings and the course of atin in 21 patients, that are typical enough to enable earl ... | 2001 | 15637986 |
| a newly discovered variant of a hantavirus in apodemus peninsulae, far eastern russia. | 2001 | 11747715 | |
| comparison of a new immunochromatographic rapid test with a commercial eia for the detection of puumala virus specific igm antibodies. | hantaviruses are associated with two human diseases: haemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). puumala virus (puuv), which is one of the hantaviruses, is a causative agent of nephropathia epidemica (ne), a mild form of hfrs. | 2001 | 11595586 |
| human immune response to puumala virus glycoproteins and nucleocapsid protein expressed in mammalian cells. | puumala hantavirus (puuv) glycoproteins g1 and g2 and nucleocapsid protein (n) were expressed in bhk-21 cells by transfection of a plasmid producing a recombinant alphavirus replicon. coexpression of g1 and g2 from separate constructs seemed to be important for the optimal folding of the glycoproteins, as evaluated by a panel of mabs detecting conformational epitopes. to evaluate the human antibody response against recombinant g1, g2 and n, several panels of sera were tested by immunofluorescenc ... | 2001 | 11596100 |
| hantavirus infections in greece--an update. | hantaviruses are emerging viruses spread worldwide and cause hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). two pathogenic hantaviruses are present in greece: dobrava/belgrade virus (dobv) and puumala virus (puuv). the predominant one is dobv, carried in greece by the rodent apodemus flavicollis. most human infections are sporadic, but epidemic outbreaks were also described. the severity of the disease ranges from a mild or moderate form to severe and fatal ... | 2001 | 11599695 |
| a lethal disease model for hantavirus pulmonary syndrome. | hantaviruses are associated with two human diseases, hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). development of vaccines and therapies to prevent and treat hfrs and hps have been hampered by the absence of a practical animal model. here we report that andes virus (andv), a south american hantavirus, is highly lethal in adult syrian hamsters. the characteristics of the disease in hamsters, including the incubation period, symptoms of rapidly progressing r ... | 2001 | 11601912 |
| expression of integrin alphavbeta3 in pig, dog and cattle. | the alphavbeta3 integrin, also known as vitronectin receptor, is an adhesive glycoprotein that promotes angiogenesis in the embryo and tumors such as melanoma. integrin alphavbeta3 is one of the receptors for adenovirus and hantavirus. there is little information on the constitutive expression of this integrin especially in animal species that are used for biomedical research. we used light and electron microscope immunocytochemistry and western blots to determine integrin alphavbeta3 expression ... | 2001 | 11642723 |
| genotyping and preparation of the recombinant nucleocapsid protein antigen of hantavirus. | to identify new recombinant antigens with potential for diagnosis of hemorrhagic fever with renal syndrome (hfrs) and establish reverse transcription-polymerase chain reaction-restriction fragments length polymorphism (rt-pcr-rflp) for genotyping of hantavirus. | 2001 | 11677760 |
| seasonal variation in prevalence of antibody to hantaviruses in rodents from southern argentina. | we conducted a small mammal trapping study to investigate temporal variation in prevalence of infection in hantavirus reservoir populations in the patagonian andes mountain range, rio negro province, argentina. rodent blood samples collected in natural and periurban habitats and at the home of an hantavirus pulmonary syndrome (hps) case patient were analysed by enzyme-linked immunosorbent assay. organ tissue samples were tested by polymerase chain reaction (pcr) and nucleotide sequence analysis. ... | 2001 | 11679129 |
| [hantavirus infection in bank voles in the natural reservoir. part 1. characteristics of an infection process in bank voles]. | the specific features of hantavirus infection in naturally infected bank voles (clethrionomys glareolus), the principal host of hantavirus of the serotype puumala, were studied during long-term observation of individually marked animals in the active focus of hemorrhagic fever with renal syndrome (hfrs) in the south of udmurtia. the infection time in the bank voles was defined by paired serum seroconversion tests. in the natural focus, hantavirus was shown to cause asymptomatic persistent infect ... | 2001 | 11680366 |
| hantavirus infections in europe. | 2001 | 11680383 | |
| the hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide waasa. | the medium segment of the tripartite negative-stranded rna genome of hantaviruses encodes for the predicted glycoprotein precursor gpc. we have demonstrated here the expression of the glycoprotein precursor of hantaan virus following transfection of mammalian cells. the cleavage of the precursor into the glycoproteins g1 and g2 followed the rules for signal peptides and seemed to occur directly at the pentapeptide motif "waasa." our data indicate that the signal peptidase complex is responsible ... | 2001 | 11689045 |
| molecular evolution of puumala hantavirus. | puumala virus (puuv) is a negative-stranded rna virus in the genus hantavirus, family bunyaviridae. in this study, detailed phylogenetic analysis was performed on 42 complete s segment sequences of puuv originated from several european countries, russia, and japan, the largest set available thus far for hantaviruses. the results show that puuv sequences form seven distinct and well-supported genetic lineages; within these lineages, geographical clustering of genetic variants is observed. the ove ... | 2001 | 11689661 |
| [hantavirus infection epidemiology in belgium]. | in europe, puumala (puu) is a hantavirus responsible for a human disease called nephropathia epidemica and its natural reservoir is the red bank vole, (clethrionomys glareolus). although the population densities and the prevalence rates of infection were high in red bank voles in southern belgium during the 1996 and 1999 epidemic years, the percentages of infected rodents were low in 1997 and 1998, when only a few positive sites were found. antibodies against puu virus were mainly detected in th ... | 2001 | 11697188 |
| hantavirus infections and their prevention. | hantaviruses are rodent-borne bunyaviruses which cause haemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome in humans. this review covers the host interactions of the viruses, including the rodent reservoirs, the clinical outcome of human infections as well as the pathogenesis and laboratory diagnosis of infections. the current stage in prophylaxis and therapy of hantaviral diseases is described and different approaches in vaccine development are discussed. | 2001 | 11709294 |
| rapid presumptive diagnosis of hantavirus cardiopulmonary syndrome by peripheral blood smear review. | hantavirus cardiopulmonary syndrome (hcps) is a rare but frequently lethal acute zoonotic viral infection in rural north america. the rapidity of progression from febrile prodrome to cardiogenic shock and noncardiogenic pulmonary edema requiring intensive care creates high diagnostic urgency and a need for a rapid screening tool. in this retrospective cohort study, 2 pathologists scored blinded peripheral blood smears from 52 patients with hcps and 128 seronegative patients referred for diagnosi ... | 2001 | 11710682 |
| [hemorrhagic fever with renal syndrome]. | puumala hantavirus is the most common hantavirus infection in western europe. the causative agent, puumala virus, is a member of the hantavirus genus in the bunyaviridae family. the natural hosts of hantaviruses are chronically, but asymptomatic infected rodents, which transmit the virus to human in their excretions. puumala virus is carried by the bank vole, clethrionomys glareolus. hemorrhagic fever with renal syndrome (hfrs) caused by puumala virus in france or belgium is very similar to the ... | 2001 | 11715607 |
| estimated incubation period for hantavirus pulmonary syndrome. | 2001 | 11716089 | |
| mesangiocapillary glomerulonephritis caused by puumala hantavirus infection. | nephropathia epidemica induced by puumala hantavirus typically causes acute reversible renal function impairment. a typical renal biopsy finding is acute tubulointerstitial nephritis with slight glomerular mesangial changes. we describe here 5 patients who developed the nephrotic syndrome during the convalescent phase of an otherwise typical acute febrile nephropathia epidemica. renal biopsy of all patients disclosed type i mesangiocapillary glomerulonephritis (mcgn). a clinical remission of the ... | 2001 | 11721157 |
| first molecular evidence for puumala hantavirus in slovakia. | we report on the first puumala hantavirus nucleotide sequence (strain opina-916) amplified from a bank vole trapped in slovakia, central europe. phylogenetic analysis of the s-segment sequence grouped the virus within the western/central european sublineage of puumala virus. in the neighborhood of the rodent trapping site two cases of human infection by the puumala virus were verified. | 2001 | 11724269 |
| do unusual site-specific population dynamics of rodent reservoirs provide clues to the natural history of hantaviruses? | between january 1995 and november 1997, longitudinal mark-recapture studies of rodent hosts of hantaviruses in a disturbed microhabitat within a shortgrass prairie ecosystem in southeastern colorado (usa) were conducted. the site was distinguished by edaphic and floristic characteristics unique to this area and associated with historical land use patterns, as well as the year-around availability of water from a functioning windmill. populations of two common rodent species that are hosts for han ... | 2001 | 11310878 |
| serologic evidence of hantavirus infection in sigmodontine rodents in mexico. | antibodies to hantaviruses in two species of sigmodontine rodents (peromyscus maniculatus and reithrodontomys sumichrasti) collected in central mexico are reported. peromyscus maniculatus, a common species throughout much of mexico, is the reservoir of sin nombre virus (snv), the etiologic agent of the great majority of cases of hantavirus pulmonary syndrome (hps) in north america. although the identity of the virus detected in p. maniculatus in mexico could not be determined by these serologic ... | 2001 | 11310896 |
| genetic and serotypic characterization of sin nombre-like viruses in canadian peromyscus maniculatus mice. | in canada, hantavirus infected deer mice (peromyscus maniculatus) have been collected from british columbia to newfoundland. partial sequencing of g1 and n protein encoding regions from canadian peromyscus maniculatus-borne hantaviruses demonstrated the existence of significant genotypic divergence among strains. phylogenetic analysis showed that sin nombre (sn)-like viruses from eastern and western canadian deer mice can be divided into at least two broad-based genogroups. sequencing of mitocho ... | 2001 | 11311430 |
| hantavirus infections: clinical presentation in the emergency room. | we present a retrospective review of hantavirus infection in the emergency department. thirteen cases of hantavirus infections with renal syndrome from july 1989 to august 1999 were analysed. the diagnosis was confirmed by detection of hantavirus antibodies in all cases. fever, chills and headaches were universally present. intense back pain was associated in 77% of the patients. thrombocytopenia, abnormal urinalysis, hypertransaminasaemia, increased lactate dehydrogenase were the principal biol ... | 2001 | 11314815 |
| molecular cloning and high-level expression of g2 protein of hantaan (htn) virus 76-118 strain in the yeast pichia pastoris km71. | hantaan viral g2 envelope gene, which is known to be one of major antigens and induce neutralizing antibodies, was cloned into expression vector phil-s1 which consists of aox1 promoter, pho1 signal sequence, his4 gene and other components. the recombined plasmid was transformed into methylotropic yeast, pichia pastoris of km71 and recombinant strains harboring multi-copy of g2 gene were selected. expression of the cloned g2 gene was confirmed with western blot analysis using anti-sera of guinea ... | 2001 | 11324753 |
| [hantavirus pulmonary and cardiovascular syndrome: epidemiology, clinical presentation, laboratory diagnosis and treatment]. | the hantavirus pulmonary and cardiovascular syndrome (hpcvs) is an emerging disease in brazil. in this study, eight confirmed cases of hpcvs were studied. all the patients presented fever and dyspnea as well as thrombocytopenia and hypoxemia. tachycardia, malaise, hypotension and lung rales occurred in 75 to 87.5% of the cases. hemoconcentration, blood cell count increased and immature neutrophils, and high levels of creatinine were observed in 75 to 87.5%. intravenous liquid infusion, the use o ... | 2001 | 11340492 |
| hantavirus pulmonary syndrome due to andes virus in temuco, chile: clinical experience with 16 adults. | to describe the clinical features and laboratory abnormalities of 16 adults with confirmed hantavirus pulmonary syndrome (hps) due to andes virus in temuco, chile. | 2001 | 11502657 |
| longitudinal studies of sin nombre virus in deer mouse-dominated ecosystems of montana. | sin nombre virus (snv), hosted by the deer mouse (peromyscus maniculatus), is the primary etiologic agent of hantavirus pulmonary syndrome (hps) in north america. to improve our understanding of the epidemiology of hps in the western united states, we conducted studies of population dynamics and snv antibody prevalence in deer mouse populations for 6 years on 12 mark-recapture grids in montana. monthly numbers of deer mice ranged from zero to over 170 on 1-hectare grids. snv antibody prevalence ... | 2001 | 11504405 |
| sex differences in immune responses and viral shedding following seoul virus infection in norway rats. | in the field, male rodents are more frequently infected with hantaviruses than females. this study examined whether patterns of immune responses against hantavirus differed between the sexes. male and female long evans rats (rattus norvegicus) were inoculated with seoul virus, and antibody and cytokine responses, as well as virus shedding were assessed. males were more likely to shed virus in saliva, to shed virus through multiple routes (saliva, urine, and feces), and to have viral rna in the s ... | 2001 | 11504409 |
| dna vaccination with the hantaan virus m gene protects hamsters against three of four hfrs hantaviruses and elicits a high-titer neutralizing antibody response in rhesus monkeys. | four hantaviruses-hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv) and puumala virus-are known to cause hemorrhagic fever with renal syndrome (hfrs) in europe and asia. htnv causes the most severe form of hfrs (5 to 15% case-fatality rate) and afflicts tens of thousands of people annually. previously, we demonstrated that dna vaccination with a plasmid expressing the seov m gene elicited neutralizing antibodies and protected hamsters against infection with seov and htnv. here, we r ... | 2001 | 11507192 |
| hbv core particles as a carrier for b cell/t cell epitopes. | in the middle 80s, recombinant hepatitis b virus cores (hbc) gave onset to icosahedral virus-like particles (vlps) as a basic class of non-infectious carriers of foreign immunological epitopes. the recombinant hbc particles were used to display immunodominant epitopes of hepatitis b, c, and e virus, human rhinovirus, papillomavirus, hantavirus, and influenza virus, human and simian immunodeficiency virus, bovine and feline leukemia virus, foot-and-mouth disease virus, murine cytomegalovirus and ... | 2001 | 11509871 |
| a chemiluminescence detection method of hantaviral antigens in neutralisation assays and inhibitor studies. | a protocol for chemiluminescence detection of hantaviral antigens in infected cell foci is described. this focus detection is based on the conversion of a substrate into a luminescent product by peroxidase-antibody conjugates; the emitted light of infected cell foci can easily be recorded by autoradiography or video imaging providing a hard copy for documentation. the main advantage of this method as compared to conventional immunochemical staining is a higher detection sensitivity due to the in ... | 2001 | 11516485 |
| virology. finally, a handle on the hantaviruses. | 2001 | 11520958 | |
| hemorrhagic fever with renal syndrome: diagnostic problems with a known disease. | hemorrhagic fever with renal syndrome (hfrs), caused by different hantaviruses, is a distinct clinical syndrome endemic in several parts of asia and europe. however, the clinical picture can sometimes be indistinguishable from that of other infectious or noninfectious diseases. in this report we describe a clinical case, which is a rare occurrence but is a prime example of the difficulties in the diagnosis of hfrs in areas with a low prevalence of the disease. | 2001 | 11526193 |
| from the centers for disease control and prevention. hantavirus pulmonary syndrome--vermont, 2000. | 2001 | 11529243 | |
| epidemiology and epizootiology of hantavirus infection in japan. | hantaviruses cause two severe human diseases: hemorrhagic fever with renal syndrome (hfrs) and hantavirus pulmonary syndrome (hps). various rodent species act as animal reservoirs for hantavirus. in japan, urban rat- (rattus norvegicus) and laboratory rat-derived human infections were reported during the 1960s and 1970s-1984, respectively. although no human cases of infection have been reported since 1984, infected urban rats have been found throughout japan, and infected grey red-backed voles ( ... | 2001 | 11544398 |
| hantavirus infection complicated with arf and spontaneous perinephric haemorrhage. | 2001 | 11549914 | |
| hantavirus pulmonary syndrome in anajatuba, maranhão, brazil. | the authors report a confirmed case of hantavirus pulmonary syndrome in the rural area of the municipality of anajatuba, state of maranhão. two other suspected cases from the same region are also described. the confirmed case involved a previously healthy young woman who died with signs and symptoms of acute respiratory insufficiency 5 days after presenting fever, myalgia and a dry cough. the patient was a student who was helping her parents with work in the fields; it was a habit of the family ... | 2001 | 11558007 |
| purification and characterization of the sin nombre virus nucleocapsid protein expressed in escherichia coli. | sin nombre virus is a member of the hantavirus genus, family bunyaviridae, and is an etiologic agent of hantavirus pulmonary syndrome. the hantavirus nucleocapsid (n) protein plays an important role in the encapsidation and assembly of the viral negative-sense genomic rna. the sin nombre n protein was expressed as a c-terminal hexahistidine fusion in escherichia coli and initially purified by nickel-affinity chromatography. we developed methods to extract the soluble fraction and to solubilize t ... | 2001 | 11570855 |
| epizootiological survey of hantavirus among rodent species in ningxia hui autonomous province, china. | hantaviral antibodies were detected in the sera from apodemus (a.) agrarius and a. peninsulae captured in ningxia province, china by several different serological diagnostic methods. a total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (ifa). among them, 19 of 191 (9.9%) sera of a. agrarius and 1 of 13 (7.7%) sera of a. peninsulae were positive for hantaviral antibodies. the other species (rattus norvegicus, mus ... | 2001 | 11590918 |
| climate variability and change in the united states: potential impacts on vector- and rodent-borne diseases. | diseases such as plague, typhus, malaria, yellow fever, and dengue fever, transmitted between humans by blood-feeding arthropods, were once common in the united states. many of these diseases are no longer present, mainly because of changes in land use, agricultural methods, residential patterns, human behavior, and vector control. however, diseases that may be transmitted to humans from wild birds or mammals (zoonoses) continue to circulate in nature in many parts of the country. most vector-bo ... | 2001 | 11359689 |
| [hantavirus pulmonary syndrome in buenos aires province]. | in argentina the first hantavirus pulmonary syndrome (hps) cases were characterized in 1995. since then, argentina is the country with the highest number of notified cases in south america. the disease is distributed in northern, southern and central regions of the country, being buenos aires the most affected province from the central region. in this study, we present seasonal and geographical distribution of hps cases in buenos aires province, the association with diverse viral lineages and th ... | 2001 | 11374136 |
| new immunochromatographic rapid test for diagnosis of acute puumala virus infection. | a new immunochromatographic rapid test, poc puumala (erilab ltd., kuopio, finland), for detection of acute-phase puumala virus (puuv) infection was developed based on a highly purified baculovirus-expressed puuv nucleocapsid protein antigen and lateral immunodiffusion techniques. after addition of sample (5 microl of serum, plasma, or fingertip blood) and buffer, puuv-specific immunoglobulin m (igm) antibodies, if present, together with the gold-conjugated anti-human igm, formed a specific color ... | 2001 | 11376049 |
| isolation of dobrava virus from apodemus flavicollis in greece. | dobrava virus (dobv) carried by apodemus flavicollis is the causative agent of severe hemorrhagic fever with renal syndrome (hfrs). dobv was isolated from an a. flavicollis mouse trapped in northeastern greece. this is the third dobv cell culture isolate in the world, clustering together with other greek dobv sequences from hfrs patients and rodents. | 2001 | 11376073 |
| physicians' database searches as a tool for early detection of epidemics. | we analyzed retrospectively the use of physician desk reference database searches to identify epidemics of tularemia, nephropathy, pogosta disease, and lyme disease and compared the searches with mandatory laboratory reports to the national infectious diseases register in finland during 1995. continuous recording of such searches may be a tool for early detection of epidemics. | 2001 | 11384534 |
| high prevalence of sin nombre virus in rodent populations, central utah: a consequence of human disturbance? | 2001 | 11384538 | |
| hantavirus seroconversion of wild-caught peromyscus during quarantine. | 2001 | 11384539 | |
| hantavirus pulmonary syndrome in the united states. | since the first outbreak of hantavirus pulmonary syndrome (hps) in 1993, understanding of the vast distribution and potential impact of hantaviruses has grown. at least 277 cases of hps have been documented in the united states. the full clinical spectrum has yet to be elucidated, and one outbreak suggested the possibility of person-to-person transmission. new research has identified the b-3 integrins as cellular receptors for hantaviruses and has determined the pivotal role of the immune system ... | 2001 | 11384556 |
| hantavirus infection induces the expression of rantes and ip-10 without causing increased permeability in human lung microvascular endothelial cells. | sin nombre virus (snv) and hantaan virus (htn) infect endothelial cells and are associated with different patterns of increased vascular permeability during human disease. it is thought that such patterns of increased vascular permeability are a consequence of endothelial activation and subsequent dysfunction mediated by differential immune responses to hantavirus infection. in this study, the ability of hantavirus to directly induce activation of human lung microvascular endothelial cells (hmve ... | 2001 | 11390609 |
| clinical characterization of dobrava hantavirus infections in germany. | there is increasing evidence that dobrava (dobv) but not hantaan (htnv) hantavirus is a hemorrhagic fever with renal syndrome (hfrs) causing agent in central europe. however, only single clinical cases of hfrs due to acute dobv infection have been described so far. we report on three male patients from a non-endemic hantavirus focus in northern germany who suffered from mild to moderate hfrs strongly resembling nephropathia epidemica. serotyping by detection of hantavirus species-specific neutra ... | 2001 | 11393382 |
| molecular epidemiology of viral pathogens and tracing of transmission routes: hepatitis-, calici- and hantaviruses. | background: the need to rapidly identify new therapeutic drugs and vaccines for clinically important viral infections has resulted in intensive study of the molecular properties of viruses. modern molecular techniques have provided tools for tracing infections and studying the evolution of viruses. objective study and design: two examples illustrating how modern molecular techniques can be used in clinical virology and molecular epidemiology (hepatitis and caliciviruses), and one example documen ... | 2001 | 11397654 |
| health assessment of u.s. military personnel deployed to bosnia-herzegovina for operation joint endeavor. | in anticipation of u.s. forces deploying to bosnia-herzegovina, plans were established to conduct medical surveillance of all military service members. this surveillance would provide the department of defense with an overview of the hospitalization and outpatient morbidity experience of u.s. forces. standardized collection of medical data from all u.s. camps using 14 diagnostic categories based on international classification of diseases, 9th revision, codes began in march 1996. special assessm ... | 2001 | 11413721 |
| predominant kidney involvement in a fatal case of hantavirus pulmonary syndrome caused by sin nombre virus. | a 27-year-old woman presented to a hospital with symptoms resembling pyelonephritis; respiratory distress did not develop until nearly a day after admission and she subsequently died. the unexplained deaths and critical illnesses project of the centers for disease control and prevention confirmed sin nombre virus infection by the results of serological testing and sequencing of the viral genome; staining of sin nombre virus antigen in the pulmonary capillaries was relatively weak. | 2001 | 11418889 |
| [regional differences and hantavirus pulmonary syndrome]. | factors related to the characteristics of hantavirus pulmonary syndrome in argentina are described. factors from different scientific fields converge to form the syndrome's analytical framework. some of these factors are the history of spatial occupation, work and production structures, human migration patterns, ethnic composition, reservoir dynamics and its relationship to the different circulating viruses, and human behavior. furthermore, the multiple factors are expressed in three ecological ... | 2001 | 11426265 |
| modifying the cellular transport of dna-based vaccines alters the immune response to hantavirus nucleocapsid protein. | puumala virus is a member of the hantavirus genus (family bunyaviridae) and is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) in europe. a genetic vaccination approach was conducted to investigate if the immune response could be modulated using different cellular secretion and/or localisation signals, and the immune responses were analysed in balb/c mice and in a bank vole infectious model. rodents vaccinated with dna constructs encoding the antigen fused to an amino ... | 2001 | 11427253 |
| truncated hantavirus nucleocapsid proteins for serotyping hantaan, seoul, and dobrava hantavirus infections. | truncated recombinant nucleocapsid proteins (rnps) of hantaan virus (htnv), seoul virus (seov), and dobrava virus (dobv) were expressed by a baculovirus system. the truncated rnps, which lacked 49 (rnp50) or 154 (rnp155) n-terminal amino acids of the nps of htnv, seov, and dobv, were able to differentiate htnv-, seov-, and dobv-specific immune sera. recombinant np50s retained higher reactivities than rnp155s and were proven useful for enzyme-linked immunosorbent assay (elisa). the elisas based o ... | 2001 | 11427545 |
| polymorphism of the cytokine genes in hospitalized patients with puumala hantavirus infection. | nephropathia epidemica (ne) is a mild type of haemorrhagic fever with renal syndrome caused by puumala (puu) hantavirus. the clinical course of ne varies from asymptomatic to fatal. the aim of this study was to establish whether polymorphisms in the cytokine genes are associated with susceptibility to and outcome of ne. | 2001 | 11427627 |
| [the worldwide challenges of "new" or reemerging communicable diseases at the dawn of the 21st century]. | in spite of the very significant advances made during the 20 th century in the prevention and the treatment of communicable diseases, infections are still today, even in developed countries, a major cause of morbidity and mortality. new infectious diseases have emerged (aids, legionellosis, exterotoxigenic e. coli, ebola fever), others have significantly reemerged (tuberculosis, diphtheria, bartonella infections) or have seen their geographic distribution widen considerably (dengue, hantavirus, ... | 2001 | 11427818 |
| [complete genome sequence analysis of the hantavirus z10 strain]. | study on the complete genome sequence of hantavirus z10 strain which has been applied for inactivated vaccine production in china, to assess its molecular characteristics and the diversity with other hantaviruses. | 2001 | 11436636 |
| antibody to sin nombre virus in rodents associated with peridomestic habitats in west central montana. | most human cases of hantavirus pulmonary syndrome are acquired in the peridomestic environment, yet studies of the ecology and infection dynamics in the reservoir host, the deer mouse (peromyscus maniculatus), have focused on sylvan populations. we describe a 2.5-year study of hantavirus infection in rodents associated with peridomestic habitats in west central montana. antibodies reactive with sin nombre virus (snv) were found in five species. overall snv antibody prevalence was highest among d ... | 2001 | 11442208 |
| incidence of hantavirus infections in belgium. | over the last two decades, and from the moment that serological detection was possible, human hantavirus infections have been documented in most european countries. this paper summarises the available data on hantavirus cases in belgium. these data enable the demonstration of the existence of a 3-year epidemic cycle in belgium, which is apparently linked to rodent population dynamics. | 2001 | 11451489 |
| interaction between molecules of hantavirus nucleocapsid protein. | intermolecular interactions of tula hantavirus n (nucleocapsid) protein were detected in the yeast two-hybrid system, prompting further attempts to study this phenomenon. using chemical cross-linking and immunoblotting it was shown that the n protein from purified virus and from infected cell lysates as well as recombinant protein produced in a baculovirus expression system are capable of forming dimers, trimers and multimers, thus confirming the capacity of the protein molecules to interact wit ... | 2001 | 11457990 |
| characterization of in vitro and in vivo antiviral activity of lactoferrin and ribavirin upon hantavirus. | mechanisms of anti-hantaviral activities of bovine lactoferrin (lf) and ribavirin (rbv) were investigated. hantavirus focus formation at 48 hr was 15% of the control in cells treated with 400 microg/ml lf for 1 hr at 37 degrees c prior to viral infection. post infection treatment with 100 microg/ml rbv also inhibited the focus formation to 2.5% of the control. combined lf pre- and rbv post-infection treatment completely inhibited focus formation. viral glycoprotein (g2) and nucleocapsid protein ... | 2001 | 11459009 |
| mystery siliguri fever solved? | 2001 | 11467154 | |
| [the worldwide challenges of "new" or reemerging communicable diseases at the dawn of the 21st century]. | in the first part of this review, aids, prion diseases, hantavirus and arbovirus infections, ebola hemorrhagic fever, legionellosis, hepatitis c, enterotoxigenic escherichia coli infections, lyme disease, tuberculosis have provided alarming examples of emerging or reemerging infectious diseases. in this second part, the stress is placed on the reemergence of diphtheria and of serious streptococcal infections, on bartonelloses, chlamydia infections, fungal infections, while malaria and cholera ar ... | 2001 | 11468579 |
| geographical distribution of rodent-associated hantaviruses in texas. | the purpose of this study was to increase our knowledge of the geographic distribution and natural host range of hantaviruses in texas, southeastern new mexico, and mexico. blood samples from 3,225 wild rodents, representing 34 species, were tested for hantavirus antibody (igg), using an enzyme-linked immunosorbent assay. hantavirus antibody was found in one or more rodents from each of 13 counties in texas, otero county in southeastern new mexico, and mexico state (central mexico). the 133 anti ... | 2001 | 11469186 |
| genetic characterization of puumala hantavirus strains from belgium: evidence for a distinct phylogenetic lineage. | puumala hantavirus (puuv) sequences were recovered from red bank voles (clethrionomys glareolus) trapped between 1996 and 1998 in four localities of southern belgium: thuin, montbliart, momignies and couvin. in addition, three puuv isolates originating from bank voles trapped in the 1980s in southern (montbliart) and northern (turnhout) belgium were genetically characterized. analysis of the complete s and partial m segment sequences showed that the belgian puuv strains constitute a genetic line ... | 2001 | 11226569 |
| prevalence of antibodies to sin nombre virus in humans living in rural areas of southern new mexico and western texas. | 2001 | 11226584 | |
| [analysis of the primary structure of hantavirus rna, detected in organs of patients that died from hfks in the republic of bashkortostan]. | fragments of the minor s and middle m segments of hantavirus genome rna were isolated by reverse transcription-polymerase chain reaction (rt-pcr) from the organs of patients who died from hemorrhagic fever with the renal syndrome (hfrs) during an epidemic outbreak of this infection in the bashkortostan republic in 1997-1998. the sizes of the resultant pcr fragments were 317 p. n. for s segment and 416 p. n. for m segment. their primary structures (genbank registration numbers aj133581 and aj1335 ... | 2001 | 11236741 |
| exotic diseases. are they spreading to a town near you? | 2001 | 11242844 | |
| [respiratory manifestations of hemorrhagic fever with renal syndrome. retrospective study of 129 cases in champagne-ardenne and lorraine]. | the contradiction between airborne transmission of puumala virus and the low rate of airway manifestations reported in hemorrhagic fever with renal syndrome (hfrs) caused by this virus led us to conduct this study to check whether the incidence of respiratory manifestations may have been underestimated. | 2001 | 11244810 |
| hantavirus. | although hantaviruses have been known to exist for over 40 years, they were thought only to be the cause of hemorrhagic fever and renal syndrome (hfrs) in europe and asia. rodents are the primary hosts of hantaviruses, and humans become infected mainly by inhalation of viral particles in the rodent excreta. the united states was thought to be spared from pathologic hantaviruses until the summer of 1993, when an outbreak of acute respiratory distress syndrome occurred in the four corners region o ... | 2001 | 11246028 |
| evaluation of a pan-reactive hantavirus enzyme immunoassay and of a hantavirus immunoblot for the diagnosis of nephropathia epidemica. | nephropathia epidemica (ne) caused by the hantavirus serotype puumala (puuv) is endemic in large parts of europe. the prognosis of this disease is usually good. however, a rapid serological diagnosis is important to differentiate ne from potentially more severe renal conditions. | 2001 | 11255099 |
| a serological survey of rural dogs and cats on the southwestern canadian prairie for zoonotic pathogens. | a survey for antibodies against agents of plague, tularemia, and rocky mountain spotted fever (rmsf), and against sin nombre hantavirus (snv), bartonella henselae and b. clarridgeiae was conducted in the summer of 1995 using serum from rural dogs and cats living in the vicinity of four public parks in southeastern alberta and southwestern saskatchewan. antibodies to all pathogens were detected in all survey areas. overall prevalence rates were 0.075 for yersinia pestis, 0.089 for francisella tul ... | 2001 | 11257996 |
| mouse mx2 protein inhibits hantavirus but not influenza virus replication. | the antiviral potential of mx2 protein remains unknown, because the mx2 gene in commonly used strains of laboratory mice is nonfunctional. our previous study showed that functional mx2 protein in some feral-origin strains was induced upon interferon treatment, was localized in the cytoplasm, and inhibited vesicular stomatitis virus replication. in the present study, we have demonstrated that the embryonic fibroblastic cells from a feral-origin strain (spr) expressed 74 kda mx2 protein, which pre ... | 2001 | 11266216 |
| the english 'sweate' (sudor anglicus) and hantavirus pulmonary syndrome. | a rapidly fatal viral infectious disease appeared in england in 1485, persisted for the summer months and disappeared as winter approached. this pattern of infection re-appeared in 1508, 1517, 1528, and finally 1551. the epidemic never returned. it had no respect for wealth or rank, and predominantly attacked males between the ages of 15 and 45 years. the incubation period was frighteningly short and the outcome normally fatal. the symptoms of acute respiratory disease and copious sweating were ... | 2001 | 11284216 |
| [epidemiology of hantaviruses in baden-wurttemberg]. | hantavirus, originally named after the hantaan river in korea, is the aetiologic agent for the hemorrhagic fever with renal syndrome (hfrs) in the asian region, in the americas for the hantavirus pulmonary syndrome (hps). in middle europe hantaviruses are responsible for the "nephropathia epidemica" (ne), a mild form of hfrs. hantaviruses belong to the family of bunyaviridae. like other members of this family their genome consists of three segments of single stranded rna (ss-rna) leading to var ... | 2001 | 11285748 |
| unexplained deaths and critical illnesses project (unex). | 2001 | 11474630 | |
| hantavirus pulmonary syndrome--vermont, 2000. | in 1993, an outbreak of an unexplained pulmonary illness occurred in the southwestern united states. this outbreak led to the first description of hantavirus pulmonary syndrome (hps), a rodentborne hantaviral infection. hantaviruses have been found in rodents in rural areas throughout the united states, but most infection has occurred in the southwest. this report describes the first hps case in vermont and underscores the importance of preventing exposure to peridomestic rodents and recognizing ... | 2001 | 11476530 |
| infection with sin nombre hantavirus: clinical presentation and outcome in children and adolescents. | sin nombre hantavirus (snv) is the leading causative agent of hantavirus cardiopulmonary syndrome (hcps) in the united states and canada. relatively few cases of hcps have involved children. this report describes the clinical characteristics of a series of pediatric cases of snv infection in the united states and canada from 1993 through march 2000. | 2001 | 11483837 |
| genetic identification and characterization of limestone canyon virus, a unique peromyscus-borne hantavirus. | hantaviruses, family bunyaviridae, are rodent-borne rna viruses that can cause hantavirus pulmonary syndrome (hps) in various regions of the americas. a coevolutionary relationship exists between hantaviruses and their specific rodent reservoir hosts; the phylogeny of the viruses generally matches that of the rodents. there are several peromyscus-borne hantaviruses, including sin nombre virus, the most common cause of hps in north america. this report describes the genetic detection and characte ... | 2001 | 11485402 |
| highly sensitive taqman pcr detection of puumala hantavirus. | an increasing number of clinical cases of hantavirus infections have been reported from various regions in asia, europe and north america. hantaviruses (family bunyaviridae, genus hantavirus) are enveloped and possess a single-stranded trisegmented rna genome of negative polarity. rodents or insectivores are natural hosts of hantaviruses and transmit the virus to humans chiefly by aerosolisation. these viruses are the causative agents of haemorrhagic fever with renal and pulmonary syndromes. in ... | 2001 | 11489422 |
| [pathogenesis of hemorrhagic fever]. | 2001 | 11496775 | |
| [hantavirus-related hemorrhagic fever in europe]. | 2001 | 11496776 | |
| dna vaccination of mice with a plasmid encoding puumala hantavirus nucleocapsid protein mimics the b-cell response induced by virus infection. | inoculation of naked dna has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. to study the humoral immune response induced by dna vaccination we cloned the entire nucleocapsid protein-encoding sequence of the puumala hantavirus strain vranica/hällnäs into the cmv promoter-driven expression unit of the plasmid pcdna3, generating pcdna3-vr1. a single dose injection of 50 microg of plasmid dna into each m. tibialis anterior of balb/c mice ... | 2001 | 11035190 |