Publications

parainfluenza virus 5-vectored vaccines against human and animal infectious diseases.	parainfluenza virus 5 (piv5), known as canine parainfluenza virus in the veterinary field, is a negative-sense, nonsegmented, single-stranded rna virus belonging to the paramyxoviridae family. parainfluenza virus 5 is an excellent viral vector and has been used as a live vaccine for kennel cough for many years in dogs without any safety concern. it can grow to high titers in many cell types, and its genome is stable even in the presence of foreign gene insertions. so far, piv5 has been used to d ...	2018	29316047
development of a genotype-by-sequencing immunogenetic assay as exemplified by screening for variation in red fox with and without endemic rabies exposure.	pathogens are recognized as major drivers of local adaptation in wildlife systems. by determining which gene variants are favored in local interactions among populations with and without disease, spatially explicit adaptive responses to pathogens can be elucidated. much of our current understanding of host responses to disease comes from a small number of genes associated with an immune response. high-throughput sequencing (hts) technologies, such as genotype-by-sequencing (gbs), facilitate expa ...	2018	29321894
development and evaluation of the rt-pcr kit for the rabies virus diagnosis.	to improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus dna by the rt-pcr technique was developed and evaluated. the analytical sensitivity of the kit was 4*10 ge per ml. high specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.	2018	29323857
an assessment of shedding with the oral rabies virus vaccine strain spbn gasgas in target and non-target species.	a safety requirement for live vaccines is investigating possible shedding in recipients since the presence of replication competent vaccine in secretions could result in direct and indirect horizontal transmission. this is especially relevant for oral rabies vaccine baits that are deliberately distributed into the environment. in the current study, survival of an oral rabies virus vaccine, spbn gasgas, was examined in excretions from different target and non-target species; red fox, raccoon dog, ...	2018	29325820
evaluation of g2 citric acid-based dendrimer as an adjuvant in veterinary rabies vaccine.	for induction of an appropriate immune response, especially in the case of an inactivated vaccine, the use of an adjuvant is crucial. in this study, adjuvanticity effect of g2 dendrimer in veterinary rabies vaccine has been investigated. a nonlinear globular g2 dendrimer comprising citric acid and polyethylene glycol 600 (peg-600) was synthesized and the toxicity was studied in vitro on the j774a.1 cell line. the adjuvanticity effect of the dendrimer was then investigated on rabies virus in nmri ...	2018	29328884
defining objective clusters for rabies virus sequences using affinity propagation clustering.	rabies is caused by lyssaviruses, and is one of the oldest known zoonoses. in recent years, more than 21,000 nucleotide sequences of rabies viruses (rabv), from the prototype species rabies lyssavirus, have been deposited in public databases. subsequent phylogenetic analyses in combination with metadata suggest geographic distributions of rabv. however, these analyses somewhat experience technical difficulties in defining verifiable criteria for cluster allocations in phylogenetic trees inviting ...	2018	29357361
integrated anatomical and physiological mapping of striatal afferent projections.	the dorsomedial striatum, a key site of reward-sensitive motor output, receives extensive afferent input from cortex, thalamus and midbrain. these projections are integrated by striatal microcircuits containing both spiny projection neurons and local circuit interneurons. to explore target-cell specificity of these projections, we compared inputs onto d1-dopamine receptor positive spiny neurons, parvalbumin-positive fast-spiking interneurons and somatostatin-positive low-threshold-spiking intern ...	2018	29359830
efficacy of ontario rabies vaccine baits (onrab) against rabies infection in raccoons.	in the us, rabies lyssavirus (rabv) only circulates in wildlife species and the most significant reservoir from a public and animal health perspective is the raccoon (procyon lotor). management of wildlife rabies relies principally on oral rabies vaccination (orv) strategies using vaccine-laden bait delivery to free-ranging target hosts, in order to reduce the susceptible population to prevent the spread of and eliminate rabv circulation. our objective was to evaluate efficacy of the ontario rab ...	2018	30037482
human rabies associated with domestic cat exposures in south africa, 1983-2018.	no abstract available.	2020	32633988
defining new pathways to manage the ongoing emergence of bat rabies in latin america.	rabies transmitted by common vampire bats (desmodus rotundus) has been known since the early 1900s but continues to expand geographically and in the range of species and environments affected. in this review, we present current knowledge of the epidemiology and management of rabies in d. rotundus and argue that it can be reasonably considered an emerging public health threat. we identify knowledge gaps related to the landscape determinants of the bat reservoir, reduction in bites on humans and l ...	2020	32911766
predicting the presence and titre of rabies virus-neutralizing antibodies from low-volume serum samples in low-containment facilities.	serology is a core component of the surveillance and management of viral zoonoses. virus neutralization tests are a gold standard serological diagnostic, but requirements for large volumes of serum and high biosafety containment can limit widespread use. here, focusing on rabies lyssavirus, a globally important zoonosis, we developed a pseudotype micro-neutralization rapid fluorescent focus inhibition test (pmrffit) that overcomes these limitations. specifically, we adapted an existing micro-neu ...	2020	32931658
updates on rabies virus disease: is evolution toward zombie virus a tangible threat?	human rabies disease is caused by rabies lyssavirus, a virus belonging to rhabdoviridae family. the more frequent means of contagion is through bites of infected mammals (especially dogs, but also bats, skunks, foxes, raccoons and wolves) which, lacerating the skin, directly inoculate virus-laden saliva into the underlying tissues. immediately after inoculation, the rabies virus enters neural axons and migrates along peripheral nerves towards the central nervous system, where it preferentially l ...	2021	33682816