| purification and concentration of rabies virus by chromatography on chemically modified porous silicates. | tissue culture rabies virus was purified by gel chromatography on chemically modified macroporous glasses (mpg). modification of the mpg surface by egg or human albumin prevented rabies virions from interaction with the glass surface so that all purified virus was eluted from the column. chemically modified mpg could be used on a preparative scale for the purification of both infectious and inactivated rabies virus, the immunogenicity of the latter having been preserved. protein content of the p ... | 1981 | 6116414 |
| multisite intradermal antirabies vaccination. immune responses in man and protection of rabbits against death from street virus by postexposure administration of human diploid-cell-strain rabies vaccine. | lymphocyte transformation, production of neutralising antibody, and the development of antirabies igg antibody were studied in ten healthy volunteers in response to 0.8 ml of human diploid-cell strain (hdcs) rabies vaccine administered on one occasion in divided doses in 8 intradermal (i.d.) sites. all ten volunteers rapidly developed substantial titres of rabies antibody, and eight of the ten had t lymphocytes that were immunologically stimulated by hdcs rabies-virus antigen. postexposure treat ... | 1981 | 6117693 |
| properties of rabies vaccine freed of neuroallergenic factor from brain tissue. | fixed rabies virus in the form of infected sheep brain suspension was freed from approx. 80% of ballast proteins, inactivated by beta-propiolactone and lyophilized. the vaccine thus obtained was devoid of neuroallergenicity when tested on guinea pigs and was highly antigenic and immunogenic. the vaccine caused no generalized reactions in volunteers; local reactions were weak and of short duration. antibody formation was intensive in all volunteers. | 1981 | 6118049 |
| commercial antirabies vaccine used as antigen in a modified enzyme-linked immunosorbent assay. | | 1982 | 6124116 |
| human rabies after late administration of human diploid cell vaccine without hyperimmune serum. | | 1982 | 6126724 |
| viraemia in rabies. | | 1982 | 6127940 |
| stability of human diploid-cell-strain rabies vaccine at high ambient temperatures. | a batch of lyophilised human diploid-cell-strain rabies vaccine was divided into three batches, which were exposed to different temperatures during their distribution to and storage at three centres in pakistan. vaccine potency after exposure to these temperatures was tested by measuring antibody response in those given the vaccine, and by three different laboratory tests. the results indicate that the vaccine retains its antigenicity for man and for laboratory tests despite continuous exposure ... | 1983 | 6132229 |
| rabies. | | 1983 | 6132275 |
| rabies vaccine prepared from the virus grown in japanese quail embryo cell cultures. | fixed rabies virus strain mniivp-74 was grown in japanese quail embryo cell cultures, concentrated by ultrafiltration and inactivated with beta-propiolactone. the resulting vaccine was markedly antigenic and immunogenic for laboratory animals. human volunteers injected with 2.0 ml vaccine on days 0, 3, 7, 14, 30 and 90 exhibited more intensive and longer antibody production than those injected daily for 14 days. | 1983 | 6133431 |
| stability of human diploid cell rabies vaccines. | | 1983 | 6134059 |
| structural proteins of fixed rabies virus in the suckling mouse brain. | structural proteins of fixed rabies virus grown in the suckling mouse brain were analysed by sds-polyacrylamide gel electrophoresis (page). the relative content of g protein to l, n and m1 proteins of brain-grown virus was much lower when compared with that of the virus grown in chick embryo fibroblasts. the results indicate that nucleocapsid complexes, consisting of l, n and m1 proteins were the predominant antigens accumulating in the brain. | 1983 | 6135337 |
| an economical regimen of human diploid cell strain anti-rabies vaccine for post-exposure prophylaxis. | vaccine regimens using 0.1 ml human diploid cell strain vaccine (hdcsv) given intradermally (id) in single and multiple sites, or with aluminum hydroxide adjuvant given subcutaneously (sc), were compared with the regimens of hdcsv and semple vaccine currently suggested by who. some groups were also given human rabies-immune globulin (hrig). neutralising antibody titres were monitored for 3 months. antibody was detected earliest in subjects given 0.1 ml hdcsv id at each of eight sites. the highes ... | 1983 | 6135830 |
| inhibition of opiate receptor-mediated signal transmission by rabies virus in persistently infected ng-108-15 mouse neuroblastoma-rat glioma hybrid cells. | acute and persistent rabies virus infection of mouse neuroblastoma-rat glioma hybrid cells (ng-108-15) results in a loss of the normal inhibiting function of opiates via the opiate receptor on hormone-stimulated adenylate cyclase activity. previous studies of these persistently infected cells have shown a decrease in the affinity of the opiate receptors for agonists without any change in the number of these receptors. we now demonstrate that persistently infected cells are unable to couple the o ... | 1984 | 6142455 |
| a new, highly immunogenic duck embryo rabies vaccine. | | 1984 | 6143152 |
| failure to achieve predicted antibody responses with intradermal and intramuscular human diploid cell rabies vaccine. | in a study to compare the immunogenicity of human diploid cell rabies vaccine (hdcv) given by intramuscular or automatic intradermal jet injection, neither method of administration resulted in antibody levels predicted by previous studies. 49 days after starting a series of three 0.1 ml doses of hdcv given intradermally, 85 volunteers had a geometric mean titre (gmt) of neutralising antibody to rabies of 1:170. 9 concurrent control subjects who received 1.0 ml doses of vaccine intramuscularly ha ... | 1984 | 6144829 |
| chloroquine prophylaxis associated with a poor antibody response to human diploid cell rabies vaccine. | | 1984 | 6145850 |
| post-exposure rabies treatment with fetal bovine kidney cell vaccine in people bitten by animals with confirmed rabies. | | 1984 | 6145906 |
| use of post-exposure intradermal rabies vaccination in a rural mission hospital. | the efficacy of single site, intradermal human diploid cell rabies vaccine (hdcv) was assessed in patients exposed to rabies, who were treated at a rural mission hospital in central thailand. intradermal hdcv, 0.1 ml on days 0, 3, 7 and 14, resulted in protective antibody levels by day 14, with measurable titres present at one year. 98% of 219 patients completed primary vaccination and 84% returned for a one-year booster. no case of rabies has been seen in those vaccinated. intradermal hdcv in s ... | 1984 | 6146863 |
| ammonium chloride and chloroquine inhibit rabies virus infection in neuroblastoma cells. brief report. | two lysosomotropic agents, ammonium chloride and chloroquine inhibit the infection of murine neuroblastoma cells by rabies virus. the experiments indicate that rabies virus infects the neuronal cells through an endosome ph dependent entry pathway. | 1984 | 6148053 |
| enzyme-linked immunosorbent assay for quantification of rabies antibodies in human sera. | a double antibody enzyme linked immunosorbent assay (elisa) was elaborated for detection of rabies antibodies in human sera. the procedure consisted of coating polyvinylchloride plates with rabbit antirabies serum followed by attachment of partially purified fixed virus and human rabies antibodies. the rabies-specific antibodies in human antisera were quantified by means of antihuman peroxidase conjugate. titration of antisera from human volunteers immunized with the "fermi" vaccine revealed exc ... | 1984 | 6151357 |
| use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. ii. the glycoprotein. | twenty-five hybridoma cultures secreted monoclonal antibodies directed against the glycoprotein of rabies or rabies-related viruses. the antibodies had different specificities for the glycoproteins of eight rabies and rabies-related viruses. they could be classified into fourteen groups which probably correspond to different antigenic determinants on the glycoproteins. these hybridomas when used in either radioimmunoassay (ria) or in neutralization tests allow differentiation of laboratory strai ... | 1980 | 6155432 |
| use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. i. the nucleocapsid protein. | twenty-one hybridoma cultures, obtained through the fusion of mouse myeloma cells with splenocytes of balb/c mice immunized with either rabies virus or mokola virus, secreted monoclonal antibodies specific for the nucleocapsid of the inducer virus. they displayed different specificities for the nucleocapsids of rabies and rabies-related viruses and could be classified into eight groups which are likely to correspond to different antigenic determinants on the nucleocapsid. four strains of fixed r ... | 1980 | 6155436 |
| antigenic variants of rabies virus. | antigenic variants of cvs-11 strain of rabies virus were selected after treatment of virus populations with monoclonal antibodies directed against the glycoprotein antigen of the virus. these variants resisted neutralization by the hybridoma antibody used for their selection. two independently mutating antigenic sites could be distinguished when five variants were tested with nine hybridoma antibodies. the frequency of single epitope variants in a cloned rabies virus seed was approximately 1:10, ... | 1980 | 6156982 |
| rabies vaccine prepared in human cell cultures: progress and perspectives. | rabies vaccine prepared in human diploid cell strains is a replacement for the previously available vaccines that are prepared in animal tissues and are less immunogenic and more reactogenic. the human cel-grown vaccine made in the united states is a split-product vaccine, whereas the vaccines made in europe are whole-virion vaccines. both types of vaccine contain concentrated and inactivated "fixed" rabies virus. when used before exposure to rabies virus, the vaccine should be given intramuscul ... | 1980 | 6158081 |
| [rabies virus persistence in vitro]. | | 1980 | 6159728 |
| persistence and variation of mangosta street rabies virus antigens during adaptation into mice. | a mangosta street rabies virus strain antigen not shared by the challenge virus standard (cvs) was identified by counterimmunoelectrophoresis. this strain antigen persisted following nine mouse passages of the virus, during which the cvs strain antigen was not acquired. these observations suggest that the antigen composition of the two virus strains is different. antigenic differences during the course of adaptation of mangosta virus were also revealed by crossed serum neutralization tests. the ... | 1980 | 6159749 |
| in vitro effect of isoprinosine on rabies virus. | the in vitro effect of isoprinosine, an antiviral compound, was evaluated on era and va319 rabies viral strains. isoprinosine diminished the number of immunofluorescent cells, viral titers, and structures involved in rabies virus replication. mechanism of antiviral action was probably mediated by modification of the polyribosome conformation and function. | 1980 | 6160794 |
| mouse model for abortive rabies infection of the central nervous system. | when adult mice were injected by the footpad route with the attenuated rabies virus era/bhk, serum- and brain-associated antibody and interferon were produced, nonspecific cytotoxic cells and virus-specific cytolytic t cells in the spleen were activated, and a nonlethal infection of the central nervous system occurred. cyclophosphamide treatment of these animals 1 day after virus infection suppressed antibody formation and induction of cytolytic t cells and resulted in a lethal infection. virus ... | 1981 | 6163717 |
| a mouse model of the pathogenesis and postexposure prophylaxis of rabies. | a mouse model for the study of postexposure prophylaxis of rabies was established. mice injected intramuscularly with a street strain of rabies virus were significantly protected from death by five daily 0.2-ml doses of inactivated rabies vaccine of chick embryo cell culture origin initiated immediately or 3 hr after infection. in these mice, a large amount of circulating interferon was induced as early as 1 hr after the first dose of vaccine and lasted until at least 12 hr but no such amount of ... | 1980 | 6163950 |
| essential role of t cells in the postexposure prophylaxis of rabies in mice. | athymic nude mice injected intramuscularly with a street stain of rabies virus were not protected against rabies by postexposure administration of beta-propiolactone-inactivated rabies vaccine. in contrast, their normal littermates were completely protected from death by the same vaccination regimens. nude mice did not produce igg antibody as a result of the vaccine during the test period of 15 days, whereas normal littermates produced igg antibody from day 5 after vaccination. however, passive ... | 1981 | 6171709 |
| immunofluorescent staining of rabies virus antigen in formalin-fixed tissue after treatment with trypsin. | formalin-fixed central nervous system tissue from clinically rabid animals was treated with 0.25% trypsin and tested for the presence of rabies virus antigen by direct immunofluorescent (if) staining. the results were comparable with those obtained from direct if staining of acetone-fixed standard smears or fresh frozen-cut sections. experiments were conducted using coded brain specimens (classified as if-negative, weakly positive, or strongly positive) and showed a specificity of 100% for secti ... | 1981 | 6172212 |
| [prevention of inter-human rabies transmission after corneal graft]. | transplantation, to a normal recipient, of a cornea taken from a donor who died of rabies encephalitis constitutes an extremely serious exposure. four cases, up to now are known of human to human transmission of rabies virus. in these four cases, the diagnosis of rabies in the donor has been made afterwards when to the recipient developed fatal rabies. we report here the first case of this type of exposure where the diagnosis of rabies in the donor could be made on the day following the transpla ... | 1981 | 6175385 |
| the use of monoclonal antibodies in virology. | | 1982 | 6177742 |
| [interferon and antirabies vaccination]. | | 1982 | 6180483 |
| frequency analysis of cytolytic t cell precursors (ctl-p) generated in vivo during lethal rabies infection of mice. i. distinction of ctl-p with different interleukin 2 sensitivity. | the aim of this study was to determine the number and state of activity of cytolytic t lymphocytes (ctl) and their precursors (ctl-p) present in vivo during the early stages of viral infection. the local response to lethal infection with rabies virus was used as a model system that is not accessible to analysis by secondary activation in vitro. the local response to alloantigen served as a control. experimental protocols were established that allow frequency estimates of in vivo antigen-triggere ... | 1982 | 6180904 |
| molecular basis of rabies virus virulence. i. selection of avirulent mutants of the cvs strain with anti-g monoclonal antibodies. | | 1982 | 6181189 |
| antigenic variants of rabies virus. | monoclonal antibodies directed either against the nucleocapsid or the glycoprotein of rabies virus were reacted by indirect immunofluorescence and mouse neutralization tests with vaccine strains and rabies field isolates of various origin. major antigenic determinants allow the grouping of viruses and minor ones the differentiation of virus strains. a protection study showed the existence in nature of certain antigenic variants against which conventional vaccines do not fully protect. the isolat ... | 1982 | 6181927 |
| [antigenic variants among street rabies virus isolates from france, africa, madagascar and asia. preliminary results with antinucleocapsid monoclonal antibodies]. | a panel of monoclonal antibodies directed against the nucleocapsid antigens of rabies virus allowed a rapid characterization of isolates by an indirect immunofluorescence test on brain smears of infected animals. analysis of 77 isolates (71 from animals and 6 from humans) showed a great diversity. isolates from different areas showed different patterns of reactivity. no relation was found between animal species and antigenic patterns specificity. | 1982 | 6181928 |
| antigenic variants of rabies virus. | the authors studied nineteen street virus isolates from different regions of canada using either anti-nucleocapsid and anti-glycoprotein monoclonal antibodies or cross-protection tests. this study only allowed us to recognize three groups of viruses with different nucleocapsid patterns, and no difference, as far as protection with a pitmann-moore vaccine is concerned, between four of these strains. | 1982 | 6181929 |
| [introduction to the study of antigenic variants of rabies virus]. | | 1982 | 6181930 |
| [antigenic variants of rabies virus in france. monoclonal antibodies]. | one hundred and sixteen rabies field isolates from france were studied with monoclonal antibodies for analysis of their nucleocapside antigenic characteristics (by direct or indirect immunofluorescence) or glycoprotein determinants (by neutralization in mice). these studies verified that nucleocapside antigens had only two variants, apparently uncorrelated to animal species or geographic areas. glycoprotein antigens had also variants which could explain partial defection in cross protection obse ... | 1982 | 6181931 |
| monoclonal antibodies study on rabies virus antigens in thailand. | | 1982 | 6183386 |
| antigenic structure of rabies virus glycoprotein: ordering and immunological characterization of the large cnbr cleavage fragments. | after rabies virus glycoprotein was treated with cnbr, the peptide mixture was fractionated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. cnbr-cleaved peptide fragments were resolved into seven peptide bands under reducing conditions and six peptide bands under nonreducing conditions. the isolated nonreduced polypeptides were further analyzed by electrophoresis under reducing conditions. the n-terminal amino acid sequences were determined for the peptides in each of t ... | 1982 | 6183450 |
| altered expression of rabies virus antigenic determinants associated with chronic infection and virulence. | | 1983 | 6185633 |
| characterization of an antigenic determinant of the glycoprotein that correlates with pathogenicity of rabies virus. | the pathogenicity of fixed rabies virus strains for adult mice depends on the presence of an antigenic determinant on the viral glycoprotein. two virus-neutralizing monoclonal antibodies have been used to identify this determinant. all pathogenic strains of fixed rabies virus bind to these antibodies and are neutralized by them, whereas nonpathogenic strains fail to react with these monoclonal antibodies and are not neutralized by them. antigenic variants of the rabies virus with altered glycopr ... | 1983 | 6185960 |
| [chemotherapy of rabies virus in tissue culture]. | | 1982 | 6186562 |
| [prophylactic and therapeutic activity of an interferon inducer in mice infected intracerebrally with rabies street virus]. | experiments in mice inoculated intracerebrally with street rabies virus demonstrated a protective effect of an interferon inducer (double-stranded rna of phage f2). a high degree of protection was observed after intracerebral inoculation of the inducer both 24 h before and 4 h after virus infection, with a considerable increase of the average life-span of the animals. intramuscular and intraperitoneal inoculation of the inducer proved ineffective. | 1983 | 6189300 |
| [effectiveness of dsrna as an interferon inducer in the intramuscular infection of mice with rabies street virus]. | a protective effect of double-stranded rna as interferon inducer was demonstrated in mice inoculated intramuscularly with street rabies virus. a significant degree of protection was observed when the inducer was inoculated intracerebrally and at the site of virus inoculation both 24 h before and 4 and 24 h after infection. inoculation of the inducer into the muscle opposite to the infected leg and subcutaneously into the back proved to be ineffective. it was established that rabies infection cou ... | 1983 | 6191440 |
| induction of antigen-specific antibody response in human peripheral blood lymphocytes in vitro by a dog kidney cell vaccine against rabies virus (dkcv). | in the present report an in vitro method for obtaining a secondary human antibody response to a dog kidney cell vaccine against rabies virus (dkcv) is described. cultures of peripheral blood mononuclear cells from normal rabies-immune and nonimmune donors were stimulated in vitro by dkcv. the production of virus-specific antibody in supernatant fluids was monitored by elisa. antibody was produced by lymphocytes from rabies-immune individuals, whereas those of nonimmune subjects consistently fail ... | 1983 | 6193180 |
| [intra-axonal circulation in peripheral nerves and its importance]. | | 1983 | 6195741 |
| intracytoplasmic neuronal inclusions in the hippocampus of non-rabid moose, alces alces (l.). | | 1983 | 6196493 |
| a new inactivated tissue culture rabies vaccine for use in man. evaluation of pcec-vaccine by laboratory tests. | a new inactivated rabies vaccine (purified chick embryo cell vaccine) has been developed using the flury lep-c 25 strain of rabies virus propagated in primary chick embryo cell cultures. the antigen was purified and concentrated by continuous density gradient centrifugation and inactivated by betapropiolactone. this vaccine was tested for innocuity, tolerability and protective capacity in a series of laboratory tests and compared with human diploid cell strain (hdc)-vaccines of similar antigenic ... | 1984 | 6199357 |
| distribution of rabies virus, interferon and interferon-mediated enzymes in the brains of virus-infected rats. | the distribution of rabies virus, interferon and interferon-mediated enzymes, pppa(2'p5'a)n synthetase (2-5a synthetase) and poly(ri) . poly(rc)-sepharose-bound protein kinase, was studied in different regions of the brains of rabies virus-infected rats. a broad range of virus infectivity was found in the brain stem, cerebellum, cortex, hippocampus and striatum. similarly, the levels of interferon were variable (2500 to 60 000 units/mg protein in tissue extracts) in the different brain regions s ... | 1984 | 6202834 |
| [effect of the interferon inducer double-stranded rna on the development of vaccinal antirabies immunity]. | experimental mice treated with an interferon inducer (double-stranded rna) and vaccine, vaccine alone, and gamma-globulin plus vaccine were used to study the time course of development of immunity to rabies by intramuscular and intracerebral challenge with street rabies virus. combined use of the interferon inducer and specific vaccine was shown to produce an earlier and more intensive immunity than vaccine alone or gamma-globulin and vaccine, and also a more marked humoral immune response. it i ... | 1984 | 6203231 |
| immunoelectron microscopic localization of rabies virus antigen in central nervous system and peripheral tissue using low-temperature embedding and protein a-gold. | a protein a-gold technique was used in conjunction with low temperature embedding to visualize ultrastructurally mature virions and sites of viral replication in the brains of rabies virus-infected mice after peripheral inoculation of virus. the association of viral profiles and gold particles with synaptic membranes, microtubules and rough endoplasmic reticulum suggested a mechanism of rabies virus transport within the central nervous system. early interactions of inflammatory cells with the vi ... | 1983 | 6203925 |
| basis of neurotropism of rabies virus: a new hypothesis. | there is evidence that rabies viruses travel along the peripheral nerves, in the axoplasm to reach the central nervous system (cns). it is hypothesised that rabies viruses are attracted electrically or magnetically by the nerve impulses to the cns. this explains why rabies viruses show specific affinity towards nerve tissue. the present hypothesis offers a new approach to the study of pathogenesis of the various neurotropic viruses. | 1984 | 6206381 |
| comparative studies of rabies and sindbis virus replication in human neuroblastoma (sym-i) cells that can produce interferon. | k-104 cells, a cloned cell line derived from a human neuroblastoma (sym-i), were induced by rabies hep-flury virus to release large amounts of interferon, and the resulting antiviral activity significantly suppressed the rabies virus replication. the role of endogenous interferon was confirmed by treatment with anti-interferon antibody which increased the yield of progeny virus. the virus yield in the second undiluted passage through k-104 cells was much less than that in the first passage, beca ... | 1984 | 6208314 |
| [monitoring antibody activity in personnel in infectious disease departments in the north bohemian region using the enc-hem sevac kit]. | | 1984 | 6209016 |
| [rabies vaccine in czechoslovakia. i. hempt's vaccine and its modification]. | | 1982 | 6215991 |
| [rabies vaccine in czechoslovakia. ii. lyophilized rabies vaccine from brain tissue inactivated by uv irradiation]. | | 1983 | 6218902 |
| [evaluation of the effectiveness of an antirabies serum purified and concentrated by the prosdis-4 method]. | the data on the use of rabies antitoxin obtained by the method ensuring the isolation of active immunoglobulin fractions are presented. the trial was carried out in the kazakh ssr and the uzbek ssr. the preparation was injected to subjects bitten by animals, in doses of 5 to 40 ml, depending on the localization of bites, the character of lesions and the age of victims. antitoxin " prosdis -4" was found to possess low reactogenicaty . during the term of observation (1 year) no cases of rabies wer ... | 1984 | 6234726 |
| production of neutralizing antibody to rabies and vesicular stomatitis viruses by hybrid cell lines. | | 1980 | 6243514 |
| evidence of infection by viruses in small british field rodents. | four populations of small wild british rodents were studied by capture--re-capture methods over a period of three years. samples of blood were taken from these animals and tested for antibodies to nine viruses. animals were removed from another 11 sites around the uk, and immunosuppressed. samples of tissue from these animals were tested for the presence of viruses by passage in laboratory mice and serum samples from some of them were tested for antibody to the nine viruses. indications were fou ... | 1980 | 6244344 |
| tuberculin response related to the administration of foot and mouth disease, brucellosis and rabies vaccines. | | 1980 | 6244875 |
| ["pharaoh" line culture of japanese quail cells as a leukosis-free system for virus reproduction]. | contamination of japanese quail, strain pharaoh, cell culture with oncogenous and infectious avian viruses was studied. the susceptibility of the embryonal cell cultures of the japanese quail, strain pharaoh, to measles, parotitis and fixed rabies viruses was also determined. it was found that the sera of pubertal quails had no antibody to rous sarcoma virus (rsv), strains brian, rsv (rav-1), schmidt-ruppin, carr-zilber, as well as to marek's disease and newcastle disease viruses. no reverse tra ... | 1980 | 6251636 |
| survey of virally mediated permeability changes. | 1. sendai virus causes permeability changes when added to freshly isolated brain cells (cerebellum or ependymal cells) or to a culture of forebrain cells. 2. sendai virus causes permeability changes when added to organ cultures of ferret lung or nasal turbinate. influenza virus causes no permeability changes under these conditions. 3. rabies virus and vesicular-stomatitis virus, in contrast with sendai virus, do not cause permeability changes in bhk cells or lettrée cells. 4. serum from patients ... | 1980 | 6258574 |
| time dependent decreases of maternal canine virus antibodies in newborn pups. | levels of passively transferred maternal antibodies to three canine viruses, rabies virus (rv), canine distemper virus (cdv) and infectious canine hepatitis (ich) virus, in serum specimens from 14 fetal pups and in serial serum specimens collected up to 45 days after whelping from 14 neonate pups were compared with levels of antibodies to these viruses in milk and sera collected concurrently from their respective dams. radioimmunoassays using rv-, cdv- and ich virus-specific antigens showed that ... | 1981 | 6262993 |
| interference induced in gl-v3 monkey kidney cells by rabies virus strains. | resistance to superinfection with vesicular stomatitis virus (vsv) occurred in gl-v3 monkey kidney cells infected with the cvs-11, pitman moore, lep flury, but not the era strain of rabies virus. specific immunofluorescent staining of intracellular rabies antigen showed that the number and size of fluorescent foci increased after the onset of interference, and that this was paralleled by increasing yields of infectious virus. although cvs-11 and era differed in their ability to induce interferen ... | 1981 | 6267182 |
| structure of the glycoprotein gene in rabies virus. | | 1981 | 6272128 |
| rabies virus decreases agonist binding to opiate receptors of mouse neuroblastoma-rat glioma hybrid cells 108-cc-15. | | 1981 | 6272776 |
| analysis of viral and defective-interfering nucleocapsids in acute and persistent infection by rhabdoviruses. | we have isolated and characterized the rna of intracellular virus nucleocapsids recovered from a number of cell cultures persistently infected with rabies virus or vesicular stomatitis virus (vsv). vsv persistent infections in bhk21, l cells and aedes albopictus (mosquito) cells generally showed the presence of large amounts of defective-interfering (di) nucleocapsid rna and much smaller amounts of standard (b) nucleocapsid rna. persistent infections of bhk21 cells by two rabies virus strains, c ... | 1982 | 6284869 |
| pseudorabies virus infection in raccoons: a review. | pseudorabies is a rarely reported disease of raccoons. laboratory and field evidence of prv infection suggests the raccoon is a "dead end" host with little opportunity for raccoon-to-raccoon spread of virus. all reported field cases have been associated closely with infected swine and swine have been considered the source of the raccoon infection. the clinical signs of prv in raccoons closely resembles those of canine distemper and rabies virus infections. infection with the latter viruses are c ... | 1982 | 6285008 |
| homology between the glycoproteins of vesicular stomatitis virus and rabies virus. | we compared the predicted amino acid sequences of the vesicular stomatitis virus and rabies virus glycoproteins by using a computer program which provides an optimal alignment and a statistical significance for the match. highly significant homology between these two proteins was detected, including identical positioning of one glycosylation site. a significant homology between the predicted amino acid sequences of vesicular stomatitis virus and influenza virus matrix proteins was also found. | 1982 | 6287013 |
| cellular response to rabies virus infection. | the effects of rabies virus on host cells were studied and compared to those obtained with another rhabdovirus, vesicular stomatitis virus [j. virol. 34, 777-781 (1980)]. we show here: (1) that rabies infection has no effect on cell morphology, while infection with vesicular stomatitis virus caused cell retraction. thus, only vesicular stomatitis virus induced a depolymerization of the microfilaments; and (2) that microtubules and microfilaments do not play a major role in rabies virus productio ... | 1982 | 6290135 |
| [protective action of undegraded and s-sulfonated 7s antibodies in 4 different experimental virus infections]. | | 1982 | 6293456 |
| [aspects of molecular biology of the rabies virus]. | | 1982 | 6295294 |
| rabies virus glycoprotein analogs: biosynthesis in escherichia coli. | the surface of rabies virus is composed of an approximately 60,000 dalton glycoprotein, in which most of the antigenic and immunogenic determinants of the virus reside. we have constructed plasmids for the direct expression in escherichia coli of the mature full length rabies glycoprotein gene and also for the expression of a glycoprotein gene which has been truncated to exclude the coding region for a hydrophobic, possibly transmembrane, domain of the protein. escherichia coli harboring the pla ... | 1983 | 6297004 |
| nucleotide sequence of a cdna clone encoding the entire glycoprotein from the new jersey serotype of vesicular stomatitis virus. | the nucleotide sequence of the mrna encoding the glycoprotein from the new jersey serotype of vesicular stomatitis virus (vsv) was determined from a cdna clone containing the entire coding region. the sequence of 12 5'-terminal noncoding nucleotides present in the mrna but not in the cdna clone was determined from a primer extended to the 5' terminus of the mrna. the mrna is 1,573 nucleotides long (excluding polyadenylic acid) and encodes a protein of 517 amino acids. only six nucleotides occur ... | 1983 | 6298453 |
| inhibition of rous sarcoma virus-induced transformation by preinfection with rhabdoviruses. | in vivo preinfection of chicks with rabies virus (rv) or vesicular stomatitis virus (vsv) ts 1026 inhibits tumour formation after superinfection with rous sarcoma virus (rsv). the degree of inhibition depends on the titre of the infecting viruses and the interval between rhabdovirus and rsv infection. in vitro, cells preinfected with vsv ts 1026 under non-permissive conditions and superinfected with rsv, are not transformed as judged by cell morphology, serum requirement for growth or the capaci ... | 1983 | 6300284 |
| ph-dependent hemolysis and cell fusion of rhabdoviruses. | human erythrocytes pretreated with fungal semialkali protease or trypsin became susceptible to hemagglutination by vesicular stomatitis virus (vsv) and rabies virus. both viruses exhibited extensive hemolytic and fusion activities against erythrocytes pretreated with these enzymes. the hemolysis and fusion were ph dependent and the activities were most apparent at ph 5.0 and decreased with increase in ph. however, vsv still exhibited slight hemolytic activity at neutral ph. hemolysis was also de ... | 1982 | 6300613 |
| activity of human serum lipoproteins on the infectivity of rhabdoviruses. | the inhibiting activity of various human serum lipoprotein classes and their lipid components on the infectivity of rhabdoviruses has been studied. the research has been carried out according to different experimental procedures on both the vesicular stomatitis virus (indiana strain), and the fixed rabies virus (cvs strain). the results obtained have shown an inhibition of the infectivity of vsv and cvs, mainly linked to the very low and low density lipoprotein classes. it has also been demonstr ... | 1983 | 6306404 |
| [recent data on early interaction between rhabdovirus and the cell]. | | 1982 | 6316285 |
| rapid diagnosis of viral infections in the central nervous system. | rapid diagnosis of viral infections in the central nervous system has become increasingly important. antiviral treatment, prevention of spread of disease and differentiation from infections caused by agents sensitive to antibiotics may be the important consequences of a virus specific diagnosis gained early in the disease. the diagnosis can be obtained by detection of virus or viral antigen in the human specimen: herpes simplex virus by electron microscopy, immunofluorescence or immunosorbent as ... | 1984 | 6320356 |
| [use of monoclonal antibodies in virology]. | | 1983 | 6322450 |
| nucleotide sequence and host la protein interactions of rabies virus leader rna. | rabies virus leader rna was detected in infected bhk-21 cell extracts by hybridization to end-labeled genomic rna. similar to the leader rna of vesicular stomatitis virus, the leader rna of rabies virus was also found to be associated with the la protein by specific immunoprecipitation with antisera from lupus patients. the 3' end of the genomic rna of rabies virus was sequenced, and the size and termination site of leader rna were determined. in addition, extension of the sequence into the nucl ... | 1984 | 6328006 |
| m13 bacteriophage vectors for the expression of foreign proteins in escherichia coli: the rabies glycoprotein. | the expression of a protein-coding dna fragment in a microorganism such as escherichia coli requires that the exogenous dna segment be inserted precisely in phase with bacterial translation initiation signals. we report the construction of derivatives of the m13 vectors m13mp7 and m13mp701 in which a hindiii site has been inserted, within the n-terminal section of the beta-galactosidase gene, in all three phases of translation. these vectors may thus be used for the expression, under the control ... | 1984 | 6330261 |
| serologic survey for selected viruses in a population of raccoons, procyon lotor (l.), in the great smoky mountains. | | 1984 | 6330384 |
| t cell responses to cleaved rabies virus glycoprotein and to synthetic peptides. | the antigenic structure of the rabies virus glycoprotein has been studied. a limited number of fragments were obtained by cyanogen bromide (cnbr) cleavage of viral glycoprotein, and eight large peptides were isolated by using sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis. these were tested for their capacity to stimulate the proliferation of nylon wool-purified t cells obtained from spleens of rabies-immune a/j mice. three peptides (cr1, cr2 plus cr2a, and cr3) stimulated antig ... | 1984 | 6332859 |
| methods of increasing the sensitivity of the haemagglutination inhibition test for rabies virus antibody. | a new method for the removal of non-specific inhibitors of rabies virus haemagglutinin has been developed. treatment with colloidal silicic acid (aerosil) or with acetone plus aerosil reduced the non-specific inhibitors in human, mouse, and dog sera to a level that was undetectable at the 1:4 starting dilution in the haemagglutination inhibition test.bromelain-treated goose erythrocytes were much more susceptible to haemagglutination by rabies virus than were untreated erythrocytes, and the sens ... | 1984 | 6335846 |
| [modification of the incubation period of the fixed babeş strain in relation to the nature of the host cells. the effects on viral titer and on the immunogenic value of rabies vaccines]. | | 1983 | 6336510 |
| rhesus diploid rabies vaccine (adsorbed), a new rabies vaccine. ii. results of clinical studies simulating prophylactic therapy for rabies exposure. | rhesus diploid-cell-strain rabies vaccine (rdrv) (adsorbed) is a new rabies vaccine intended for use in man. sixty volunteers were given five doses of rdrv at 0, 3, 7, 14, and 28 days to simulate prophylactic treatment of persons exposed to a rabid animal. thirty-five volunteers were given commercial high-titer rabies immune globulin, 20 iu/kg, before the first dose of rdrv, and 25 were given rdrv without prior rabies immune globulin. antibody responses at 14, 28, and 42 days were comparable wit ... | 1983 | 6341639 |
| epidemiologic analysis of antigenic variations of street rabies virus: detection by monoclonal antibodies. | the nucleocapsid antigen of 204 strains of street rabies virus, which originated in europe, africa and asia, was analyzed by fluorescent antibody staining technique with a panel of 20 monoclonal antibodies specific for nucleocapsid of rabies and rabies-related viruses. a definite pattern of reactivity was observed with strains of the same geographic origin with the exception of strains originating from madagascar, thailand and iran which were more diversified. mice immunized with a vaccine prepa ... | 1983 | 6342369 |
| [identification of silvatic and arctic rabies strains using monoclonal antibodies]. | | 1983 | 6346689 |
| an in vivo and in vitro study of rabies virus infection of the rat superior cervical ganglia. | in the attempt to develop a homogeneous neuronal model to study rabies pathogenesis in vivo and in vitro, the superior cervical ganglia (scg) were chosen because of their unique features. in vivo infection of the scg was attempted by inoculation of fixed rabies virus into the anterior eye chamber. however, viral by this route as well as intracerebrally failed to infect this neuronal organ in adult rats whereas the infection was poorly efficient in 24 hours newborn rats. dissociated cell cultures ... | 1983 | 6347127 |
| rabies subunit vaccines. | | 1983 | 6348210 |
| immunofluorescent examination of the skin of rabies-infected animals as a means of early detection of rabies virus antigen. | correlations were made on immunofluorescence positivity to antirabies conjugate between cranium-derived nerve fibers in skin and traditional samplings of brain tissue from several species and illness categories of animals with naturally acquired rabies. the overall correlation of results from all categories was about 98% (n, 104) for those that were brain positive and 100% (n, 99) for those that were brain negative. some animals that ultimately developed rabies were found to have immunofluoresce ... | 1983 | 6355152 |
| experimental rabies in skunks: mechanisms of infection of the salivary glands. | striped skunks (mephitis mephitis) were inoculated into the right submandibular salivary gland with street rabies virus. they were killed at various times after inoculation and several tissues were examined by immunofluorescence and light microscopy. right and left superior cervical, nodose and trigeminal ganglia, medulla oblongata and at least three regions of right and left submandibular salivary glands were examined by the fluorescent antibody technique. intracerebral titrations of salivary g ... | 1983 | 6357414 |
| [comparison of 4 technics for serologic titration of antibodies against rabies virus in dogs]. | four different serological techniques were used for the determination of antibody levels against rabies virus in 55 vaccinated street dogs: mouse neutralization test (reference), rapid fluorescent focus inhibition test, plaque reduction test and immunoenzymatic test (protein a). the results obtained with each of the last three methods were compared with those obtained with the reference test: correlation coefficients were, respectively, 0.810, 0.812 and 0.682, each being significantly correlated ... | 1983 | 6358227 |
| enzyme immunoassay for rabies antibody in hybridoma culture fluids and its application to differentiation of street and laboratory strains of rabies virus. | a rapid and sensitive enzyme immunoassay is described for detecting rabies antibody in hybridoma culture fluids. glass fiber filter disks were used to immobilize gamma-irradiated mouse neuroblastoma cells infected with street or laboratory strains of rabies virus. bound rabies-specific antibody was detected by reaction with horseradish peroxidase-labeled goat anti-mouse immunoglobulin g. the assay was performed in a 96-well filtration device developed by cleveland et al. (j. clin. microbiol. 15: ... | 1984 | 6365963 |
| the acetylcholine receptor as a cellular receptor for rabies virus. | characterization of specific host cell receptors for enveloped viruses is a difficult problem because many enveloped viruses bind to a variety of substrates which are not obviously related to tissue tropisms in the intact host. viruses with a limited cellular tropism in infected animals present useful models for studying the mechanisms by which virus attachment regulates the disease process. rabies virus is a rhabdovirus which exhibits a marked neuronotropism in infected animals. limited data su ... | 1983 | 6367238 |