Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| sulfated components of enveloped viruses. | the glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35so4(-2), whereas the nonglycosylated proteins are not. this was shown for the hn and f glycoproteins of sv5 and sendai virus, the e1 and e2 glycoproteins of sindbis virus, and for the major glycoprotein, gp69, as well as for a minor glycoprotein, gp52, of rauscher leukemia virus. the minor glycoprotein of rauscher leukemia virus is more highly sulfated, with a ratio of 35so4- [3h]glucosamine abou ... | 1975 | 170420 |
| virion trascriptase activity differences in host range mutants of vesicular stomatitis virus. | three types of conditional lethal mutant were isolated from wild-type vesicular stomatitis virus, new jersey serotype, after mutagenization by 5-fluorouracil: (i) conventional temperature-sensitive (ts) mutants, which form plaques at 31 c but not at 39 c; (ii) conventional host range mutants (hr ce), which grow in bhk but not in secondary chicken embryo cells; and (iii) temperature-dependent host range mutants (td ce), which form plaques both at 31 and 39 c on bhk cells but only at 31 c on chick ... | 1975 | 170423 |
| coupled in vitro transcription and translation of vesicular stomatitis virus messenger rna. | the virion transcriptase (nucleosidetriphosphate: rna nucleotidyltransferase, ec 2.7.7.6) of vesicular stomatitis virus was fully active when ribonucleoprotein cores from purified virions were added to cell-free protein synthesizing systems of eukaryotic origin. synthesis of mrna was linear for at least 3 hr and the newly synthesized viral mrna was efficiently utilized for the synthesis of viral proteins n (nucleoprotein), ns, and m (matrix); small amounts of a putative g (glycoprotein protein p ... | 1975 | 170604 |
| covalently linked message and anti-message (genomic) rna from a defective vesicular stomatitis virus particle. | 1975 | 171415 | |
| inhibition by aurintricarboxylic acid and polyethylene sulfonate of rna transcription of vesicular stomatitis virus. | the in vitro activity of the ribonucleoprotein-dependent rna transcriptase of vesicular stomatitis virions was found to be completely inhibited by low concentrations of aurintricarboxylic acid (ata) and polyethylene sulfonic acid (pes) when these inhibitors were added before the start of the rna polymerase reaction. however, if rna synthesis was allowed to occur before ata or pes was added, rna synthesis continued for a short time (10 min or less) in the presence of either inhibitor at a concent ... | 1975 | 171445 |
| method of examining viral rna metabolism in cells in culture: metabolism of vesicular stomatitis virus rna. | a method is described for radioactively labeling viral rna and then quickly halting further incorporation of radioactive precursor into rna so that the fate of the labeled rna can be followed. small complementary rnas synthesized during vesicular stomatitis virus infection in the presence of cycloheximide do not metabolize to virion length molecules when protein synthesis inhibition is reversed. | 1975 | 171456 |
| studies on the in vivo and in vitro messenger rna species of vesicular stomatitis virus. | 1975 | 171836 | |
| experimental vesicular stomatitis virus infection: ultrastructural pathology. | 1975 | 172368 | |
| studies on the asymmetric arrangement of membrane-lipid-enveloped virions as a model system. | lipids of bhk 21 cells (baby hamster kidney) grown in tissue culture were labelled with radioactive fatty acids. the enveloped vesicular stomatitis virus was propagated in this host cell type. the virions were purified by density gradient centrifugation. neuraminidase treatment of the intact virions led to a complete transformation of hematoside [n-acetylneuraminosyl(alpha2-3)lactosyl(beta1-1)ceramide] into lactosylceramide, with identical labelling of the ceramide portion in hematoside of the u ... | 1975 | 172423 |
| viral replication and interferon production in fetal and adult ovine leukocytes and spleen cells. | peripheral blood leukocyte and spleen cell cultures derived from adult sheep and from third-trimester (107 to 145 days of gestation) and second-trimester (70 to 98 days of gestation) fetal lambs were examined for their ability to support viral replication and to produce interferon. bluetongue virus, herpesvirus hominis type 2, and chikungunya virus failed to replicate in either leukocyte or spleen cell cultures derived from adult ewes or in cultures from second- or third-trimester fetal lambs. s ... | 1975 | 172452 |
| biosynthesis of mouse interferon by translation of its messenger rna in a cell-free system. | a fraction of mouse rna containing messenger rna coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. this product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse cells; (2) its antiviral activity was species specific; (3) its activity was completely neutralized by mouse anti-interferon serum. the synthesis of interferon in this cell-free syste ... | 1975 | 172890 |
| effect of tetraethyl thiuram disulfide (disulfiram) on the multiplication of enveloped viruses. | disulfiram at concentrations between 0.1 and 0.3 mm inhibits the multiplication of semliki forest virus (sfv), fowl plague virus (fpv), newcastle disease virus (ndv), vesicular stomatitis virus (vsv), and pseudorabies virus (prv), when administered 1 hour before and during adsorption. there is, however, no inhibition of virus multiplication, when the drug is added after adsorption onto chick embryo cells. disulfiram interferes neither with the receptors of the virus nor of erythrocytes, and it d ... | 1975 | 173261 |
| on the mechanism of neurotropism of vesicular stomatitis virus in newborn hamsters. studies with temperature-sensitive mutants. | the virulence of temperature-sensitive mutants of vesicular stomatitis virus (vsv) injected subcutaneously into newborn hamsters was positively correlated with their tendency to generate revertants and with their leakiness in cultured hamster embryo fibroblasts maintained at 37 degrees c, the measured body temperature of the animals under our experimental conditions. the complementation group of the mutants seemed important only in that it tended to determine reversion frequency and leakiness. o ... | 1975 | 173795 |
| inhibition of cellular protein synthesis by simultaneous pretreatment of host cells with fowl plague virus and actinomycin d: a method for studying early protein synthesis of several rna viruses. | a method is described for analysis of viral protein synthesis early after infection when minute amounts of viral proteins are effectively concealed by large amounts of produced host-specific proteins. the method is superior to a radioimmune assay, since all virus-induced proteins can be measured independent of their immunological reactivity. host-specific protein synthesis can be suppressed by infection with fowl plague virus. addition of actinomycin c 1.25 h postinfection does not prevent this ... | 1975 | 173875 |
| differential inhibition of vesicular stomatitis virus polypeptide synthesis by hypertonic initiation block. | synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infected cells to hypertonic medium. it is concluded that the relative sensitivity of individual viral polypeptide synthesis to hypertonic initiation block is independent of the site of ... | 1975 | 173890 |
| 5'-terminal sequence of vesicular stomatitis virus mrna's synthesized in vitro. | unmethylated or methylated 12 to 18s mrna's synthesized in vitro by the virion-associated rna polymerase of vesicular stomatitis virus contain the 5'-terminal hexanucleotide sequence g(5')ppp(5')apapcpapgp...or m 7g(5')-ppp(5')apmapcpapgp..., respectively. the implication of these results in relation to the regulation of transcription in vesicular stomatitis virus is discussed. | 1975 | 173891 |
| virus-dependent glycosylation. | the oligosaccharides of the membrane glycoproteins of sindbis virus, vesicular stomatitis virus, and rous sarcoma virus were compared on the basis of apparent size and sugar composition. it appears that each virus acquires a different set of oligosaccharides during growth in a single type of cell. | 1975 | 173894 |
| inhibition of oncornavirus functions by 2'-azido polynucleotides. | the 2'-azido analogs of poly(u) and poly(c), poly(duz) [poly(2'-azido-2'-deoxyuridylic acid)], and poly-(dcz [poly(2'-azido-2'-deoxycytidylic acid)], were found to inhibit the rna-directed dna polymerase (reverse transcriptase) activity of murine leukemia (moloney, rauscher) and sarcoma (moloney) virus, and feline leukemia (theilen) and sarcoma (gardner) virus, while under the same conditions the unsubstituted parent compounds failed to do so. in addition, poly(duz) and poly(dcz) inhibited the r ... | 1975 | 47174 |
| inhibition of viral transcriptase by immunoglobulin directed against the nucleocapsid ns protein of vesicular stomatitis virus. | in search of an anti-transcriptase, antibody was raised in rabbits to partially purified, soluble ns protein present in cytoplasmic extracts of cells infected with the indiana serotype of vesicular stomatitis (vsind) virus. this antiserum gave specific reactions of identity by agar immunodiffusion with both cytoplasmic and virion ns protein. ns antiserum also preferentially precipitated ns 3-h-labeled protein from infected cytoplasmic extracts, whereas anti-whole vsind virion serum also precipit ... | 1975 | 49440 |
| influence of interferon on the synthesis of virus particles in oncornavirus carrier cell lines. iii. survey of effects on a-, b- and c-type oncornaviruses. | the effect of interferon on the synthesis and release of a-, b- and c-type viruses by oncornavirus carrier lines was studied. murine cell lines were selected which carry either of these viruses and are sensitive to the antiviral effect of interferon, as measured by inhibition of vesicular stomatitis virus. release of c-type virus was found to be highly sensitive. release of b-type virus, on the contrary, was only marginally inhibited. synthesis of intracisternal a-type particles was finally not ... | 1975 | 50292 |
| immunologically specific production of interferon in cultures of rabbit blood lymphocytes: association with in vitro tests for cell-mediated immunity. | lymphocytes of animals with delayed hypersensitivity produce mediators of cellular immunity when challenged in vitro with specific antigen. among these are macrophage migration inhibitory factor (mif) and interferon (if). nonspecific mitogens also induce the production of these lymphokines. in the following study leukocytes and column-purified lymphocytes of the same peripheral blood sample from tuberculin (purified protein derivatives [ppd])-sensitive rabbits were concurrently cultured in mediu ... | 1975 | 53204 |
| vesicular stomatitis virus (measles) pseudotypes: tool for demonstrating defective measles infections. | mixed infection of vero cells with measles and vesicular stomatitis viruses gives rise to phenotypically mixed infections virus particles possessing the properties of pseudotypes. similar results were obtained when vesicular stomatitis virus was used to superinfect a defective measles system. | 1975 | 61189 |
| inhibition of vesicular stomatitis virus replication by poly(2'-fluoro-2'-deoxyuridylic acid). | poly(2'-fluoro-2'-deoxyuridylic acid) is known to be an effective inhibitor of the deoxyribonucleic acid polymerase found within the oncornaviruses. this synthetic polynucleotide was found to inhibit the replication of vesicular stomatitis virus in mouse l cells. the polymer was shown to be capable of inhibiting the viral ribonucleic acid (rna)-dependent rna polymerase, and it is proposed that this is the mechanism of antiviral activity. the following observations support this viewpoint: (i) the ... | 1975 | 174488 |
| polykaryocytosis induced by vesicular stomatitis virus infection in bhk-21 cells. | cytopathological effects by vesicular stomatitis virus (vsv) infection were studied in several cell lines. marked polykaryocyte formation was observed in monolayers of certain strains of bhk-21 cells infected with vsv. the bhk-21-kb cells were found to be the most susceptible to virus-induced cell fusion. this type of cell fusion was related to intracellular growth of the viruses, and strong cytolytic changes were found to occur following the development of large multinucleated giant cells. the ... | 1975 | 174526 |
| ultrastructural changes in bovine lingual epithelium infected with vesicular stomatitis virus. | vesicular stomatitis virus was inoculated into the dorsal lingual epithelium of three cows. the reaction that developed in 72 h was characterized by severe acute diffuse glossitis with intercellular edema and necrosis of keratinocytes. virions budded from the plasma membrane and were in the intercellular spaces. reduplication of desmosomes was a prominent alteration, and normal desmosomes were within the cytoplasm. intracytoplasmic desmosomes appeared to be formed by endocytosis after breaks occ ... | 1975 | 180646 |
| lethal synergy between sarcoma-180/tg cells and vesicular stomatitis virus in mice. | an interaction between sarcoma-180/tg cells and vesicular stomatitis virus in adult mice resulted in the rapid onset of extensive mortality. this interaction, termed lethal synergy, occurred only at early stages of ascites induction in animals with no prior virus contact. a significant sparing effect conferred by the serotonin antagonist dibenamine was reversed by the administration of serotonin. the cause of death was not determined, but a mechanism involving hypersensitivity is indicated. | 1975 | 174141 |
| a comparative study of the surface projections of different strains of vesicular stomatitis virus. | 1975 | 213529 | |
| an attenuated variant of eastern encephalitis virus: biological properties and protection induced in mice. | wild type eastern equine encephalitis virus (e) was compared with a mutant (em) derived from it. the latter was tested as an attenuated vaccine in mice. they differed in the following properties: em formed smaller plaques on chick embryo (ce) cell monolayers and, unlike e, did not plaque on mouse embryo (me) monolayers. futher, em had a longer latent period and attained a lower peak titer than e after infection of ce cells, was more senssitive than e to chick interferon, and was less virulent fo ... | 1975 | 1119939 |
| structural proteins of two salmonid rhabdoviruses. | purified infectious hematopoietic necrosis (ihn) virus and the virus of haemorrhagic septicaemia (vhs) (egtved virus) each contain five structural proteins which were designated l, g, n, m-1, and m-2. the ihn viral polypeptides have molecular weights estimated to be 157,000, 72,000, 40,000, 25,000 and 20,000, respectively, whereas those of vhs viral polypeptides are estimated to be 157,000 74,000, 41,000, 21,500, and 19,000, respectively. the carbohydrate composition of the glycoprotein (g) was ... | 1975 | 1167914 |
| physico-chemical and serological characterization of five rhabdoviruses infecting fish. | viruses isolated from fish with viral haemorrhagic septicaemia (vhs), infectious haematopoietic necrosis (ihn), spring viraemia of carp (svc), swim-bladder inflammation (sbi) and pike fry disease (pfd) have been grown to high titre in fathead minnow cells. while our preparations of the ihn, svc, sbi and pfd viruses showed typical rhabdovirus morphology with bullet-shaped particles and distinct surface projections, the vhs virus preparations had a less typical rhabdovirus morphology but were pleo ... | 1975 | 1170278 |
| fish rhabdoviruses: comparative study of protein structure. | proteins from four fish rhabdoviruses have been studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the viruses were: trout viral hemorrhagic septicemia (vhs), infectious hematopoietic necrosis virus (ihn), spring viremia virus of carp (svc), and the pike fry rhabdovirus (pfr). for the two salmonid viruses (vhs-ihn), gel electrophoresis indicated the proteins, with molecular weights estimated to be 190,000, 80,000, 38,000, 25,000, and 19,000, respectively. the electrophoretic p ... | 1975 | 1171263 |
| temperature-sensitive mutants isolated from hamster and canine cell lines persistently infected with newcastle disease virus. | evidence is presented which confirms that temperature-sensitive (ts) mutants with an rna- phenotype are spontaneously selected in persistent infection of cell lines with newcastle disease virus. persistently infected bhk-21 cells, maintained since 1973, produce no interferon and are completely susceptible to vesicular stomatitis virus. persistent infection of a canine kidney cell line (mdck) terminated with destruction of all cells at about 100 days. even under these conditions, a high proportio ... | 1975 | 1237634 |
| dependence of translation on 5'-terminal methylation of mrna. | many eukaryotic cell and viral mrnas contain 5'-terminal m7g(5')ppp(5')n. methylation is important for the in vitro translation of vesicular stomatitis virus and reovirus mrnas. unmethylated viral mrnas are methylated by cell-free extrascts of wheat germ and l cells to form 5'-terminal structures of the type, m7gpppn and m7gpppnm, respectively. rabbit globin mrna also contains 5'-terminal 7-methylguanosine (m7g). removal of the m7g by beta-elimination decreases translation. the efficient binding ... | 1976 | 782922 |
| enhancement of animal viruses growth on rk13 cells pretreated with 6-azauridine. | in these experiments a technique for enhancing the virus replication in tissue culture (rk13 cells) has been used. the method consisted in growing the cells in presence of mug 0.4-0.8 of 6-azauridine/ml of cellular suspension until the monolayers were formed. this pretreatment enhances the replication of several animal viruses with increased infectious titers (vesicular stomatitis, equine arteritis, equine rhinopneumonitis, aujeszky disease and myxomatosis virus) and with increased yield of tota ... | 1976 | 828059 |
| [reasons for lowered resistance to krebs 2 ascitic carcinoma in mice in a state of hyporeactivity (tolerance) to a viral interferon inducer]. | decreased resistance to krebs-2 ascitic carcinoma was demonstrated in mice which were in the state of hyporesponsiveness (tolerance) to virus interferon inducer (ndv) and increased resistance of the control mice to transplantation of tolerant carcinoma cells. manifestations of tolerance in peritoneal and carcinoma cells were shown to be identical: interferon production and reproduction of vesicular stomatitis virus were inhibited, the number of viable cells was reduced, masked infection with ndv ... | 1976 | 936575 |
| characterization of miniature pig kidney cells and their resistance to chlamydial infection. | a miniature pig kidney cell line has been established from porcine fetuses taken aseptically by hysterectomy and maintained for more than 50 passages in eagle's minimal essential medium containing 10% heat-inactivated newborn calf serum. cell transfers were performed each week. primary and serially passaged cells were found to be highly refractory to infection by chlamydia trachomatis strains tw-3, bour, and lgv 440l and chlamydia psittaci strains meningopneumonitis and 6bc and insusceptible to ... | 1976 | 944002 |
| defective bud formation in human cells chronically infected with subacute sclerosing panencephalitis virus. | human prostate cells chronically infected with the mantooth strain of subacute sclerosing panencephalitis (sspe) virus multiply normally, fuse only occasionally to form giant cells, and yet have twisted intracytoplasmic nucleocapsids. these cells are able to support replication of vesicular stomatitis virus, although they release only small amounts of sspe virus. to determine why carrier cells do not produce virus, they were examined with techniques for surface replication, freeze-fracturing, an ... | 1976 | 957482 |
| high resolution polyacrylamide gradient gel electrophoresis. | a method was developed for high resolution electrophoresis of proteins in linear gradient (3 to 30%) polyacrylamide gel rods in a neutral phosphate buffer containing 0.1% sodium dodecyl sulfate. well-defined protein zones were observed and improved resolution was attained especially for low molecular weight proteins in preparations containing a variety of polypeptides, e.g. viruses that are often separated by continuous gel methods. electropherograms of continuous (8%) and gradient (3 to 30%) ge ... | 1976 | 1005359 |
| influence of 5'-terminal m7g and 2'--o-methylated residues on messenger ribonucleic acid binding to ribosomes. | removal of 80% of the 5'-terminal 7-methyl-guanosine (m7g) from methylated reovirus mrna by beta elimination results in a concomitant loss of the ability to bind to wheat germ ribosomes. the mrna molecules that retain the m7g account for most of the residual binding. removal of the m7g from all molecules in preparations of methylated reovirus and vesicular stomatitis virus mrna reduces the extent of binding to wheat germ ribosomes from 80% to 5-7%. in the reticulocyte lysate, however, significan ... | 1976 | 1009086 |
| a comparison of the extents of methylation of vesicular stomatitis virus messenger rna. | 1976 | 174297 | |
| isolation of the matrix (membrane) protein of vesicular stomatitis virus by gel filtration in guanidine hydrochloride. | the l, n and m proteins of vesicular stomatitis virus (vsv) were resolved from each other by gel filtration in the presence of 6 m-guanidine hydrochloride. amino acid analysis for purified m protein of vsv showed that its chemical composition differed from those of influenza and sv5 m proteins. | 1976 | 187728 |
| ecological associations of vesicular stomatitis virus in rural central america and panama. | the principal objective of this study was to determine ecological associations of vesicular stomatitis virus (vsv)- new jersey and vsv-indiana in rural central america and panama. two types of information were linked: the results of neutralizing antibody tests performed on sera from 3,232 lifetime residents of 189 rural study communitities of central america and panama, and ecological characteristics of the study communities as determined from natural resource atlases. the major finding was that ... | 1976 | 188350 |
| decreased interferon production by leukocytes in marasmus. | interferon production by leukocytes in culture was investigated in nine severely marasmic infants and 31 well-nourished controls. the production of interferon was induced with newcastle disease virus and assayed in vero cells challenged with vesicular stomatitis virus. marasmic infants produced significantly less interferon than controls. it is suggested that the finding may be the result of a lymphocyte defect induced by malnutrition and could help to explain the increased frequency and severit ... | 1976 | 180790 |
| rescue of vesicular stomatitis virus from homologous and heterologous interferon-induced resistance in human cell cultures by poxviruses. | in human cell cultures the ability of poxviruses to rescue vesicular stomatitis virus from human interferon-induced resistance was significantly more efficient than the ability to rescue it from simian interferon-induced resistance. the sensitivity of the poxvirus to interferon was not related to its ability to rescue vesicular stomatitis virus. | 1976 | 181327 |
| phenotypic mixing of vesicular stomatitis virus and d-type oncornavirus. | after mixed infection of cells with vesicular stomatitis virus (vsv, thermolabile mutant tl 17) and oncornavirus type d, phenotypically mixed virions are formed containing the vsv genome and oncornavirus and vsv envelope. the virions are thermostable and have serologic characteristics of both viruses. | 1976 | 181332 |
| temperature-sensitive mutants of vesicular stomatitis virus are conditionally defective particles that interfere with and are rescued by wild-type virus. | temperature-sensitive (ts) mutants of vesicular stomatitis virus belonging to complementation groups i, ii and iv inhibited the replication of wild-type vesicular stomatitis virus when mixed infections were carried out in bhk21 cells at 32, 37, and 39.5 c. the group iv mutant (ts g 41) was most effective in this regard; wild-type virus yields were inhibited almost 1,000-fold in mixed infections with this mutant at 32 c. in the case of group i and ii mutants, inhibition of wild-type virus replica ... | 1976 | 181590 |
| comparative membrane microviscosity of fish and mammalian rhabdoviruses studied by fluorescence depolarization. | the microviscosity of the hydrophobic region of the membrane of infectious hematopoietic necrosis virus was determined using fluorescence depolarization analysis of the probe 1,6-diphenyl-1,3,5-hexatriene and was found to be much lower at 37 c than that of another rhabdovirus, vesicular stomatitis virus. however, the microviscosity of this fish virus at 18 c, the temperature at which it was grown, corresponded to the microviscosity of vesicular stomatitis virus at 37 c. data obtained with the fi ... | 1976 | 181597 |
| role of temperature-sensitive mutants in persistent infections initiated with vesicular stomatitis virus. | noncytocidal persistent infections at 37 c of mouse l cells (lvsv) with infective b particles of vesicular stomatitis virus (vsv) could be established only in the presence of large numbers of defective interfering (di) particles. under these conditions, there was a rapid spontaneous selection of temperature-sensitive (ts) virus. at 10 days there was an increase to 17.8% in the frequency of ts clones in the virus population; by 17 days this frequency had reached 85.2%, and by 63 days 100% of the ... | 1976 | 181599 |
| mechanisms of vesicular stomatitis virus-induced cytopathic effects. i. early morphologic changes induced by infectious and defective-interfering particles. | 1976 | 181906 | |
| mechansims of vesicular stomatitis virus-induced cytopathic effects. ii. inhibition of macromolecular synthesis induced by infectious and defective-interfering particles. | 1976 | 181907 | |
| status spongiousus resulting from intracerebral infection of mice with temperature-sensitive mutants of vesicular stomatitis virus. | mice infected intracerebrally (i.c.) with wild-type (wt) vsv or temperature-sensitive (ts) mutants, ts 11, ts 22, ts 31 and ts 41, were studied for the development of histopathological lesions in the central nervous system (cns). mice infected i.c. with wt vsv exhibited histopathological lesions consisting principally of occasional foci of perivascular mononuclear cell infiltration and rare foci of necrosis. all wt vsv infected mice died within 2 days of i.c. inoculation. in contrast, mice infec ... | 1976 | 182197 |
| enveloped viruses as model membrane systems: microviscosity of vesicular stomatitis virus and host cell membranes. | the fluorescence probe 1,6-diphenyl-1,3,5-hexatriene was used to study and compare the dynamic properties of the hydrophobic region of vesicular stomatitis virus grown on l-929 cells, plasma membrane of l-929 cells prepared by two different methods, liposomes prepared from virus lipids and plasma membrane lipids, and intact l-929 cells. the rate of penetration of the probe into the hydrophobic region of the lipid bilayer was found to be much faster in the lipid vesicle bilayer as compared with t ... | 1976 | 182211 |
| [induction of dolykaryocytes by vesicular stomatitis virus in xc rat cells]. | v.s.v. induced polycaryocytes in rat embryonic fibroblasts, transformed by the prague strain of sarcoma rous (xc cells). this fusion is strictly dependent on the expression of the viral genome and is probably due to the incorporation of viral antigens in the cell membrane. the integrity of cellular rna synthesis is however not required. the fusion is probably due to a membrane structure characteristic of these transformed cells. | 1976 | 182411 |
| augmented immunogenicity of tumor cell membranes produced by surface budding viruses: parameters of optimal immunization. | membranes prepared from tumor cells infected with surface budding viruses are much more immunogenic than membranes from uninfected tumor cells. factors affecting immunization with membranes from virus-infected tumor cells were studied. preparations made with influenza virus were clearly superior to those prepared with vesicular stomatitis virus (vsv). membranes infected with vsv were maximally immunogenic at a dose equivalent to a 10% cell pack whereas influenza-virus-infected membranes were imm ... | 1976 | 182646 |
| multiplication of vesicular stomatitis virus in the leafhopper peregrinus maidis (ashm.), a vector of a plant rhabdovirus. | vesicular stomatitis virus (vsv) was found to multiply efficiently in whole peregrinus maidis (ashm.). the leafhopper vector of maize mosaic virus (mmv), a plant rhabdovirus. insects were inoculated with vsv by means of a microsyringe, collected at 1-day intervals and tested individually for the presence of virus. exponential virus multiplication occurred within the first 4 days, reaching titres of 10(6) p.f.u. per insect in days 5 to 10 after inoculation. these observations show that a common h ... | 1976 | 182911 |
| analysis of in vitro transcription products of intracellular vesicular stomatitis virus rna polymerase. | the intracellular transcriptase complex of vesicular stomatitis virus-infected l cells synthesized rna complementary to the entire infectious virus genome at either 37 degrees c or 28 degrees c in vitro. not all sequences were present at the same frequency, however; copies of that segment of the genome common to the lt defective particles were present at 20 to 100 times higher frequently than copies of the genome segment common to the st defective particle. the less frequent region was transcrib ... | 1976 | 183012 |
| translation of individual host mrna's in mpc-11 cells is differentially suppressed after infection by vesicular stomatitis virus. | infection of mpc-11 mouse plasmacytoma cells by vesicular stomatitis virus results in 30 to 35% reduction in [35s]methionine incorporation into total proteins within 30 min postinfection. by 6 h postinfection, total protein synthesis is reduced by 80 to 90%. however, even by 30 min postinfection, a differential suppression of the synthesis of individual host protein is observed. the synthesis of the immunoglobin g (igg) heavy chain (h), and, in particular, the synthesis of igg light chain (l), i ... | 1976 | 183015 |
| an ultrastructural study of central nervous system disease produced by wild-type and temperature-sensitive mutants of vesicular stomatitis virus. | outbred swiss mice 3 to 4 weeks of age were injected intracerebrally with wild-type vesicular stomatitis virus or its temperature-sensitive (ts) mutants ts 11, ts 22, ts 31, and ts 41. brain and spinal cord were then studied for pathologic changes by electron microscopy. all mice infected with wild-type vesicular stomatitis virus died within 2 days of inoculation. diffuse ependymal alterations often culminating in necrosis in brain and especially spinal cord and rare foci of necrosis and mononuc ... | 1976 | 183052 |
| studies on the in vitro transcription and translation of vesicular stomatitis virus mrna. | 1976 | 183350 | |
| quantitative studies on adsorption, elution, and haemagglutination of vesicular stomatitis virus. | radioactively-labelled vsv, both indiana and new jersey serotypes, were rapidly and efficiently adsorbed onto goose erythrocytes, but only within a narrow range of ph and at low temperatures. under optimal conditions for adsorption, both infectivity and haemagglutinating activity of vsv were reduced. a basic difference between the two serotypes was identified: adsorption and haemagglutination of the indiana serotype were stable even at ph 9.0 and at 37 degrees c, whereas new jersey serotype was ... | 1976 | 183632 |
| ultrastructure of mouse periventricular and choroid plexus tissues in experimental vesicular stomatitis virus infection. | the ultrastructure of the periventricular and choroid plexus tissues was studied in the three-week-old mouse, infected with the vesicular stomatitis virus (vsv) by the intranasal route. the vsv reached the periventricular ependymal lining late in infection. it was carried within the cytoplasm of myelinated axons and their sheath cells. inflammatory cells, although few in numbers, may have also contributed to the virus dissemination. once in the periventricular tissues the vsv entered the cells f ... | 1976 | 183634 |
| a unique rna species involved in initiation of vesicular stomatitis virus rna transcription in vitro. | purified virions of vesicular stomatitis virus (vsv) are capable of synthesizing two distinct types of virus-specific rna in vitro. the first consists of several viral mrnas which have been previously shown to contain the blocked 5' terminal sequence gpppapapcpapgp and 3' terminal poly(a). the second type of rna has an unblocked 5' terminus and does not contain poly(a) stretches long enough to bind to oligo (dt)-cellulose columns. it migrates in 20% polyacrylamide gels as a single homogeneous pe ... | 1976 | 183891 |
| characterization of a spontaneous undifferentiated carcinoma from an african green monkey (cercopithecus aethiops). | an adult male african green monkey (cercopithecus aethiops) with an undifferentiated carcinoma, probably originating from the nasal mucosa, was received from the akron, ohio zoo. cultivation of this tumor in vitro resulted in a mixture of fibroblastic and epithelial cells which was subsequently separated using differential trypsinization. the neoplastic nature of the cultured epithelial cells was verified by their ability to transplant into athymic nude, or antithymocyte serum-treated mice, wher ... | 1976 | 184033 |
| assay of bovine interferons in cultures of the porcine cell line ib-rs-2. | an assay for bovine interferons has been developed using the porcine cell line ib-rs-2 and a bovine enterovirus, cbv-d, as challenge virus. the method is based on estimation of cytopathic effect measured by uptake of neutral red. teh assay is simple, sensitive, and reproducible. a comparative test of different viruses in ib-rs-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. vesicular stomatitis viru ... | 1976 | 184049 |
| rhesus monkeys kidney cells persistently infected with simian virus 40: production of defective interfering virus and acquisition of the transformed phenotype. | monolayer cultures of llc-mk2 rhesus monkey kidney cells became persistently infected with simian virus 40 (sv40) when infected at a multiplicity of infection of 100 plaque-forming units/cell. a stable carrier state developed characterized by extensive viral proliferation without obvious cytopathic effect other than the slow growth of these cultures. by 11 weeks all cells produced the sv40 t antigen. in contrast, less than 5% of the cells produced v antigen. virus-free clonal isolates were obtai ... | 1976 | 184052 |
| induction of murine p30 by superinfecting herpesviruses. | the interaction of endogenous type c viruses with superinfecting herpes simplex virus type 2 (hsv-2) was investigated in two murine cell lines. replication of hsv-2 was suboptimal in random-bred swiss/3t3a cells and, in initial experiments, infection with a low virus-to-cell ratio resulted in carrier cultures with enhanced murine leukemia virus (mulv) p30 expression. immunofluorescence tests with swiss/3t3a cells productively infected with hsv-2 also showed hsv-associated cytoplasmic antigens an ... | 1976 | 184296 |
| glycosylation sites of vesicular stomatitis virus glycoprotein. | detailed analysis on deae-sephadex of the tryptic digestion products of the glycoprotein from vesicular stomatitis virus grown in hela suspension cultures revealed the presence of two major and several minor sugar-labeled species. the minor tryptic glycopeptides were converted to one of the two major glycopeptide species by treatment with neuraminidase. thus, vesicular stomatitis virus glycoprotein contains only two oligosaccharide side chains that are heterogeneous in their sialic acid content. | 1976 | 184302 |
| comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and l cells. | a comparison of the ability of vesicular stomatitis virus (vsv) to generate and replicate defective interfering (di) particles in primary chick embryo (ce) and mouse l cells was investigated as a means of analyzing host control over di-particle synthesis and interfering capacity. serial undiluted passage of vsv in ce and l cells indicate that vsv-di particles are generated and (or) replicate with greater efficiency in ce than in l cells. when di particles accumulate in l cells, they are able to ... | 1976 | 184896 |
| temperature-sensitive mutants of vesicular stomatitis virus: viral rna synthesis in cells infected with mutants belonging to complementation group i. | the rna polymerase in cells infected with three group i mutants of vesicular stomatitis virus has been examined. mouse l cells were incubated at the permissive temperature (30 degrees c) for a few hours after infection to allow the development of secondary transcription. the temperature dependence of the secondary transcription system was determined from the incorporation of labelled uridine, in the presence of cycloheximide, at 30 and at 38 degrees c, the later temperature being non-permissive ... | 1976 | 184905 |
| viral spread in the presence of neutralizing antibody: mechanisms of persistence in foamy virus infection. | several viruses were categorized on the basis of their ability to spread from cell to contiguous cell and form plaques in the presence of antiviral antibody. herpes simplex virus, cytomegalovirus, and vaccinia, measles, and foamy viruses were able to spread in the presence of neutralizing antibody, whereas coxsackievirus, encephalomyocarditis virus, vesicular stomatitis virus, mumps virus, and simian virus 5 failed to spread. a detailed study of one of these virus groups (simian foamy viruses) s ... | 1976 | 185150 |
| effect of neutral red and light on herpesvirus hominis type 1 in cell culture. | various concentrations of neutral red were added to monolayers of muscle-skin fibroblasts after adsorption of herpesvirus hominis type 1. the concentration necessary to reduce plaque counts was found to be 10(-5.5) m. at the same time, the minimal toxic concentration of neutral red for muscle-skin fibroblasts was determined by the concentration that reduced the plaques of a challenge virus, vesicular stomatitis virus, that was applied after treatment with neutral red and light. the minimal toxic ... | 1976 | 185301 |
| analysis of the rna species isolated from defective particles of vesicular stomatitis virus. | serial high multiplicity passage of a cloned stock of vesicular stomatitis virus was found to generate defective interfering particles containing three size classes of rna, with sedimentaiton coefficients of 31 s, 23 s and 19 s. the 31 s and 23 s rna species were found to be complementary to both the 12 to 18 s and 31 s size classes of vsv mrnas. the 19 s class of rna was found to be partially base-paired. all three rna species were found to contain ppap at their 5' termini. | 1976 | 185328 |
| effects of interferon on cell and virus growth in transformed human cell lines. | the anticellular and antiviral effects of human leukocyte interferons were studied in vitro in the transformed human embryonic cell lines. rsa and rsb. the growth of these cells was inhibited and they began to deteriorate about 48 h after treatment with 500 units/ml of interferon. when interferon was washed out within 48 h, their growth recovered gradually. the effects of interferon on cell growth depended on the amount of interferon added per cell. a subline, named ifr, was isolated which grows ... | 1976 | 185329 |
| synthesis of rna by mutants of vesicular stomatitis virus (indiana serotype) and the ability of wild-type vsv new jersey to complement the vsv indiana ts g i-114 transcription defect. | the ability of certain vesicular stomatitis virus (vsv; indiana serotype) temperature-sensitive (ts) mutants to synthesize intracellular viral complementary rna (vcrna) at permissive or nonpermissive temperatures for productive infections has been investigated. mutants belonging to complementation groups ii, iii, and v synthesize rna at nonpermissive temperature in amounts essentially equivalent to that obtained at permissive temperatures. mutant ts g i-114 possesses a thermolabile transcriptase ... | 1976 | 185410 |
| enhanced growth and plaquing of rabies virus in static chick embryo cell culture. | the 7-day egg passage line of hep flury strain of rabies virus was inoculated to primary chick embyro (ce) cells prepared in different ways to compared efficiencies of viral growth and plaquing. special care to minimize cellular damage due to trypsin at the step of monodispersion and sowing a comparatively large number of cells for monolayer preparation were required for rabies plaquing, whereas such cares were not necessary for plaquing of vesicular stomatitis virus. plaque number and size were ... | 1976 | 185442 |
| [mechanism of resistance of a clone isolated from l cells chronically infected with the virus of vesicular stomatitis]. | the results of the study on antiviral immunity acquired by l cells to vesicular stomatitis virus are presented. we failed to detect the presence of the indicator virus in the resistant culture by means of virological, electron microscopic or cytochemical methods. molecular hybrization experiments demonstrated the lack in the nuclear dna of sequences homologous to vesicular stomatitis virus rna. | 1976 | 185809 |
| specific inactivation of herpes simplex virus by silver nitrate at low concentrations and biological activities of the inactivated virus. | the infectivities of herpes simplex virus types 1 and 2 were inactivated by silver nitrate at concentrations of 30 mum or less, which did not affect at all the infectivities of hemagglutinating virus of japan, vesicular stomatitis virus, poliovirus, vaccinia virus, and adenovirus. the inactivated virus retained the capability of adsorbing to the cell, with an adsorption kinetics quite similar to that of intact virus, and of inducing the concanavalin a agglutinability in the infected cells, where ... | 1976 | 185946 |
| 13c-nmr studies of the membrane structure of enveloped virions (vesicular stomatitis virus). | the mobility of the lipids in the bilayer of the envelope of vesicular stomatitis virus has been probed over its complete space by the biosynthetic incorporation of [n-13ch3]- choline as a probe for the polar head groups and [3-13c]- and [11-13c] oleic acid and [16-13c]- palmitic acid for the hydrophobic region of the bilayer. these precursors were effectively incorporated as established by the concomitant administration of the same precursors in radioactive form. spin lattice relaxation time me ... | 1976 | 186376 |
| long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. | bhk 21 carrier cells persistently infected with vsv indiana for over 2 years have been shedding generally very low levels of mature infectious virus or mature t particles (averaging less than one-hundredth p.f.u./cell/day) yet most cells are producing virus antigens and are resistant to homologous superinfection. however, large amounts of biologically active t particle rnp can be recovered from cytoplasmic extracts of these carrier cells even at times when they are shedding no detectable infecti ... | 1976 | 186559 |
| rna synthesis in bhk 21 cells persistently infected with vesicular stomatitis virus and rabies virus. | virus-induced rna synthesis was studied in bhk 21 cells persistently infected with vesicular stomatitis virus (vsv) and rabies virus by labelling rna synthesized in the presence of antinomycin d. during persistent infection the species of messenger rna synthesized were similar in size and relative proportions to those seen during acute infection, but there were some minor differences. full-sized b virion rna was generally not detected during persistent infection, and new species (probably di vir ... | 1976 | 186560 |
| homologous interference induced by a temperature-sensitive mutant derived from an hvj (sendai virus) carrier culture. | homologous interference between a temperature-sensitive small plaque mutant (hvj-pb) derived from an hvj (haemagglutinating virus of japan - the sendai strain of parainfluenza i virus) carrier culture of bhk cells and the original wild-type virus (hvj-w) has been investigated. prior infection of llcmk2, hela, bhk or mouse l cells with hvj-pb, both at permissive and non-permissive temperatures, for 24 h resulted in a reduced yield of superinfecting hvj-w, reflecting a smaller number of cells capa ... | 1976 | 186564 |
| association of vesicular stomatitis virus proteins with hela cell membranes and released virus. | the association of vesicular stomatitis virus proteins with intracellular and plasma membranes was examined by pulse and pulse-chase labeling of virus-infected hela cells with [35s]methionine and separation of cell homogenates into three major membrane fractions in discontinuous sucrose gradients. the glycoprotein g was primarily associated with rough endoplasmic reticulum-like membranes after short radioactive pulses (2 to 4 min) but accumulated in the plasma membrane-enriched fraction and the ... | 1976 | 186639 |
| glycosylation of vesicular stomatitis virus glycoprotein in virus-infected hela cells. | glycosylation of the envelope glycoprotein of vesicular stomatitis virus was examined using virus-infected hela cells that were pulse-labeled with radioactive sugar precursors. the intracellular sites of glycosylation and the stepwise elongation of the carbohydrate side chains of the g protein were monitored by membrane fractionation and gel filtration of pronase-digested glycopeptides. the results with short pulses of sugar label (5 to 10 mtein linkage (glucosamine and mannose) are added to g w ... | 1976 | 186640 |
| asymmetric distribution of phosphatidylethanolamine in the membrane of vesicular stomatitis virus. | the membrane-impermeable reagent trinitrobenzenesulfonate has been shown to react only with the surface components of vesicular stomatitis virus (vsv) membranes. when the amount of phosphatidylethanolamine (pe) available to modification by trinitrobenzenesulfonate in intact virions was determined, it was found that 36% of the total membrane pe was converted to the trinitrophenyl derivative. the same proportion of the total membrane pe was reactive after removal of the surface glycoprotein by try ... | 1976 | 186641 |
| in vivo assembly and maturation of vesicular stomatitis virus. | previous studies on vesicular stomatitis virus (vsv) maturation in infected cells have utilized in vitro cell cultures. the present study is, to our knowledge, the first in vivo analysis of vsv-cell interaction in the central nervous system of weaning outbred swiss mice. intracerebral inoculation of wild-type vsv resulted in rapid viral replication in brain and spinal cord. by immunoflourescence, viral antigens were first seen in ependymal cells of brain and spinal cord and soon thereafter in su ... | 1976 | 186663 |
| functional and ultrastructural studies of the effects of human interferon on cell membranes of in vitro cultured cells. | the effect of human leukocyte interferon on cultured u-amnion cells was examined, and several biological parameters were registered. multiplication of vesicular stomatitis virus and the virus-produced cytopathogenic effect was prevented. the growth rate of uninfected cells was reduced, as well as the spontaneous release of 3h-uridine. these effects were observed following treatment with 10 units of interferon per ml. no morphological alterations could be detected by scanning electron microscopy ... | 1976 | 187008 |
| in vitro interferon production by bovine tissues: effects of hydrocortisone. | the effect of hydrocortisone on bovine interferon production in vitro was studied. infectious bovine rhinotracheitis virus was used as an inducer. interferon was assayed by the plaque-reduction method in bovine fetal kidney cultures, using vesicular stomatitis virus as challenge virus. hydrocortisone decreased interferon production in bovine fetal spleen and peripheral blood leukocyte cultures. hydrocortisone did not decrease interferon production by bovine alveolar macrophages, in 1 experiment. ... | 1976 | 187092 |
| in vitro interferon production by bovine tissues: induction with infectious bovine rhinotracheitis virus. | the interferon-inducing ability of infectious bovine rhinotracheitis (ibr) virus was determined in tissue cultures of bovine origin inoculated with untreated and ultraviolet (uv) irradiated ibr viruses. interferon was assayed by the plaque-reduction method in bovine fetal kidney (bfk) cell cultures, using vesicular stomatitis virus as challenge virus. highest interferon concentrations were produced by cultures of bovine fetal (bf) spleen cells and aveolar macrophage cultures derived from adult c ... | 1976 | 187093 |
| biological and electron microscopic studies on the phenotypic mixing of the thermolabile mutant of vesicular stomatitis virus, tl-17, with avian rna tumor viruses. | a thermolabile as well as thermosensitive mutant of vesicular stomatitis virus, vsv-tl-17, could be thermostabilized by phenotypic mixing with avian rna tumor viruses (atv). biological, immunological and morphological studies revealed that this effect is due to a replacement of the thermolabile projections of the vsv by the avian tumor viral projections. the arrangement of projections of vsv and atv on phenotypically mixed viria was studied by electron microscopy. a-type particles were detected ... | 1976 | 187154 |
| the pathogenesis of vesicular stomatitis virus, serotype indiana, in aedes aegypti mosquitoes, after imbibition of a viremic blood meal. | this study showed that vesicular stomatitis virus (indiana) in most instances was not capable of replicating in aedes aegypti when imbibed by the mosquitoes on a viremic host. rapid inactivation of the virus was observed in some cases within 24 hours after imbibition. attempts to demonstrate virus inactivation by midgut contents in vitro were not successful. | 1976 | 187155 |
| vesicular stomatitis virus plaque production in monolayer cultures with liquid overlay medium: description and adaptation to a one-day, human interferon-plaque. | vesicular stomatitis virus forms discrete, microscopic plaques in stationary cultures of the wish amnion cell line. microplaque formation is rapid, reproducible, and easily quantitated, occurs at temperatures ranging from 33 to 40 degrees c, and does not require a semisolid overlay. wish cells, however, are less sensitive to vesicular stomatitis virus than are chicken embryo, 3t6, or vero cells. wish amnion cells also are highly sensitive to the antiviral effects of human interferon, and a quant ... | 1976 | 187619 |
| blocked and unblocked 5'termini in vesicular stomatitis virus product rna in vitro: their possible role in mrna biosynthesis. | 1976 | 190645 | |
| the size and the cistronic origin of defective vesicular stomatitis virus particle rnas in relation to homotypic and heterotypic interference. | 1976 | 176367 | |
| characterization and translation of methylated and unmethylated vesicular stomatitis virus mrna synthesized in vitro by ribonucleoprotein particles from vesicular stomatitis virus-infected l cells. | ribonucleoprotein particles isolated from extracts of vesicular stomatitis virus (vsv) -infected l cells synthesized in vitro four classes of polyadenylated rna sedimenting at 29s, 19s, 17s, and 13s. when synthesized in vitro in the presence of the methyl donor s-adenosyl methionine, these rna species contained the following 5'-terminal structures: (i) m7g5ppp5'ampap(70%) ; (ii) m7g5'ppp5'ampampnp (20%) and (iii) pppap (10%). in the presence of the methylation inhibitor s-adenosylhomocysteine, h ... | 1976 | 176426 |
| factors involved in the generation and replication of rhabdovirus defective t particles. | previous indications that cloned b virions might be genetically predisposed to generate a particular defective t particle are shown to be inaccurate. t particle generation was found to be a much more random process than was previously believed. we show that the previously observed generation of particular sizes of t particles by b virion pools is due to the random generation of t particles during preparation of first-passage pools of cloned b virions, and these breed true during the additional p ... | 1976 | 176445 |
| oligosaccharide moieties of the glycoprotein of vesicular stomatitis virus. | vesicular stomatitis virus contains a single structural glycoprotein whose carbohydrate sequences are probably specified by the host cell. the glycopeptides derived by pronase digestion of the glycoprotein of vesicular stomatitis virus grown in hela cells have an average molecular weight of 1,800. there are multiple oligosaccharide chains on the vesicular stomatitis virus glycoprotein with protein-carbohydrate linkages that are cleaved only by strong alkali under reducing conditions, suggesting ... | 1976 | 176458 |
| host restriction of friend leukemia virus coat protein synthesis. | fv-1 gene-mediated host restriction of friend leukemia virus replication was investigated in terms of coat protein synthesis. by using the assay of pseudotype formation with vesicular stomatitis virus. it was shown that under restricting growth conditions the availablity of leukemia virus coat protein for pseudotype formation was decreased. these studies appear to eliminate a pure assembly defect as the mechanism of fv-1 host restriction. | 1976 | 176469 |
| chromosomally depleted interspecific hybrid cell clones selected with cytotoxic antisera: utilization in the study of control of murine leukemia virus host-range. | a chromosomally stable mouse-chinese hamster hybrid cell line was subjected to five rounds of selection with cytotoxic antisera raised in rabbits against either the parental mouse 3t3 cells or the parental chinese hamster wg-1 cells. routine karyological analysis of clones isolated at each stage of serum selection revealed that treatment with either serum resulted in a limited loss of chromosomes (compared to the untreated hybrid cell cultured in parallel) and that the pattern of chromosome loss ... | 1976 | 176536 |
| cell killing by viruses. iii. the interferon system and inhibition of cell killing by vesicular stomatitis virus. | 1976 | 176779 | |
| parental g protein reincorporation by a vesicular stomatitis virus temperature-sensitive mutant of complementation group v at nonpermissive temperature. | 1976 | 176787 |