Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cell killing by viruses. i. comparison of cell-killing, plaque-forming, and defective-interfering particles of vesicular stomatitis virus. | 1974 | 4361450 | |
| studies on the in vitro adenylation of rna by vesicular stomatitis virus. | 1974 | 4371099 | |
| interferon-mediated increased lysis of l-cells by vesicular stomatitis virus. | 1974 | 4371530 | |
| electron microscopy of single-stranded rna from vesicular stomatitis virus. | 1974 | 4371687 | |
| protein kinase and phosphoproteins of vesicular stomatitis virus. | protein kinases of similar but not identical activity were found associated with vesicular stomatitis (vs) virions grown in mouse l cells, primary chicken embryo (ce) cells, and bhk-21 cells, as well as being present in vs virions grown in hela and aedes albopictus cells. the virion kinase preferentially phosphorylated the nucleocapsid ns protein in vitro and to a lesser extent the envelope m protein. other virion proteins were phosphorylated in vitro only after drastic detergent treatment. part ... | 1974 | 4359419 |
| carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus grown in four mammalian cell lines. | the carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus has been determined for virus grown in four different mammalian cell lines. the glycoprotein contains mannose, galactose, n-acetylglucosamine, and neuraminic acid as the major carbohydrate components, whereas n-acetyl-galactosamine and fucose are present in lesser amounts. the glycoprotein contains approximately 9-10% carbohydrate regardless of the host cell in which it is synthesized. small quantitative diff ... | 1974 | 4372602 |
| glycoprotein fragment associated with vesicular stomatitis virus after proteolytic digestion. | 1974 | 4372791 | |
| in vitro synthesis of methylated messenger rna by the virion-associated rna polymerase of vesicular stomatitis virus. | 1974 | 4374314 | |
| lysosomal enzyme activity in poliovirus-infected hela cells and vesicular stomatitis virus-infected l cells: biochemical and histochemical comparative analysis with computer-aided techniques. | 1974 | 4374503 | |
| [virological, cytological and karyological characteristics of clones, isolated from l cell cultures chronically infected with vesicular stomatitis virus]. | 1974 | 4374815 | |
| antibody to viruses affecting cattle in commercial tissue culture grade fetal calf serum. | commercial fetal calf serum (fcs) for tissue culture use was tested for neutralizing activity against several viruses which affect cattle. certain lots of fcs contained no neutralizing activity, whereas other lots contained neutralizing activity to several viruses. it was concluded that the neutralizing activity found in certain lots of sera was due to specific antibody and that its presence could be most easily explained by the contamination of the fcs with serum from postcolostral bovine serum ... | 1974 | 4358857 |
| rhabdovirus replication in enucleated host cells. | infection of enucleated tc-7 monkey cells with rabies virus resulted in the synthesis of virus-directed rna and the production of rabies antigens but not of infectious virus. the yield of infectious vesicular stomatitis virus from enucleated tc-7 cells, on the other hand, was almost as high as that from intact cells. inhibition of the mitochondrial functions of enucleated cells by treatment with ethidium bromide did not influence the development of rabies antigens or the production of infectious ... | 1974 | 4367906 |
| [incorporation of sv40-tumor specific transplantation antigen (sv40-tsta) in vesicular stomatitis virus grown in sv40 transformed hamster cells (author's transl)]. | 1974 | 4368160 | |
| virus replication in enucleate cells: vesicular stomatitis virus and influenza virus. | the requirement of the presence of a nucleus for the replication of vesicular stomatitis virus and influenza virus has been examined by following the growth and development of these viruses in enucleate bs-c-1 cells. vesicular stomatitis virus replicates normally in enucleate cells with the rate of production of infectious virus, the amount of virus-specific protein synthesis, and the type of proteins produced being essentially the same in nucleate and enucleate cells. influenza virus does not r ... | 1974 | 4359299 |
| in vitro synthesis of proteins by membrane-bound polyribosomes from vesicular stomatitis virus-infected hela cells. | membrane-bound polysomes from vesicular stomatitis virus (vsv)-infected hela cells synthesize predominantly three proteins in an in vitro protein synthesizing system. these three proteins have different molecular weights than the viral structural proteins, i.e., 115,000, 88,000, and 72,000. addition of preincubated l or hela cell s10 or hela cell crude initiation factors stimulates amino acid incorporation and, furthermore, alters the pattern of proteins synthesized. stimulated membrane-bound po ... | 1974 | 4368799 |
| inhibition of a vesicular stomatitis virus mutant by rifampin. | rifampin reversibly inhibits the intracellular replication of a vsv mutant, whereas a revertant variant selected from this viral population, as well as the wild strain, are not affected by this drug. rifampin inhibits transcriptase activity of the sensitive mutant only and, consequently, total viral rna synthesis decreases significantly in the cells. | 1974 | 4368821 |
| the rna of defective vesicular stomatitis virus particles in relation to viral cistrons. | 1974 | 4369375 | |
| phosphorylation of vesicular stomatitis virus in vivo and in vitro. | the structural protein, ns, of purified vesicular stomatitis virus (vsv) is a phosphoprotein. in infected cells phosphorylated ns is found both free in the cytoplasm and as part of the viral ribonucleoprotein (rnp) complex containing both the 42s rna and the structural proteins l, n, and ns, indicating that phosphorylation occurs as an early event in viral maturation. vsv contains an endogenous protein kinase activity, probably of host region, which catalyzes the in vitro phosphorylation of the ... | 1974 | 4359301 |
| [growth of a transplantable mast-cell neoplasm in the mouse inhibited by vesicular stomatitis virus (author's transl)]. | 1974 | 4369577 | |
| characterization of vesicular stomatitis virus nucleocapsids. i. complementary 40 s rna molecules in nucleocapsids. | 1974 | 4369854 | |
| effect of "aquasol a", vitamin a, and "tween 80" on vesicular stomatitis virus plaque formation and on interferon action. | 1974 | 4369949 | |
| persistent noncytocidal vesicular stomatitis virus infections mediated by defective t particles that suppress virion transcriptase. | infectious b virions of vesicular stomatitis virus were 100% lethal to bhk(21) (baby hamster kidney) cells when infecting alone, and persistent noncytocidal infection could not be achieved with cloned b virions alone. however, a mixture of b virions and homologous, short, defective, interfering particles (t particles) of a temperature-sensitive mutant of the virus regularly established persistently infected, noncytocidal carrier cultures. a long t particle was generated during establishment of t ... | 1974 | 4370255 |
| vesicular stomatitis virus envelope glycoprotein alterations induced by host cell transformation. | 1974 | 4370551 | |
| temperature-sensitive mutants of vesicular stomatitis virus: comparison of the in vitro rna polymerase defects of group i and group iv mutants. | when tested in vitro, certain temperature-sensitive (ts) mutants of vesicular stomatitis virus (vsv) belonging to complementation groups i and iv appear to have defects in the virion-bound polymerase. to obtain further information concerning the nature of these defects, representative mutants were dissociated by the method of s. emerson and r. wagner (1972), and their supernatant (s) and pellet (p) fractions were tested for transcriptase activity when combined with the p and s fractions, respect ... | 1974 | 4370958 |
| maturation of vesicular stomatitis virus: electron microscopy of surface replicas of infected cells. | 1974 | 4364829 | |
| a temperature-sensitive mutant of vesicular stomatitis virus with two abnormal virus proteins. | 1974 | 4364877 | |
| virion-bound protein kinase in semliki forest and sindbis viruses. | semliki forest virus and sindbis virus (alphaviruses belonging to the togavirus group) grown in bhk-21 cells possessed very low levels of virion-associated protein kinase activity. for comparison, vesicular stomatitis virus, also grown in bhk-21 cells, contained a virion-bound protein kinase which had a specific activity 80 times greater than that of the alphaviruses. the alphavirus protein kinase was unmasked by the nonionic detergent nonidet p-40 but was not activated by cyclic nucleotides. ph ... | 1974 | 4364899 |
| inactivation of herpes simplex virus by concanavalin a. | the infectivity of herpes simplex virus type 1 (hsv-1) was inactivated after treatment with either concanavalin a (cona) or periodate. phytohemagglutinin, wheat germ agglutinin, pokeweed mitogen, and neuraminidase failed to inactivate the virus. the effect of cona could be specifically inhibited or reversed by the addition of alpha-methyl-d-glucoside or alpha-methyl-d-mannoside. evidence was obtained that hsv-1 inactivated by cona could adsorb to host cells. viral aggregation was not a major mec ... | 1974 | 4364903 |
| ribonucleic acid synthesis of vesicular stomatitis virus. vi. correlation of defective particle rna synthesis with standard rna replication. | 1974 | 4365257 | |
| mechanism of sindbis virus-induced intrinsic interference with vesicular stomatitis virus replication. | heterologous viral interference is induced by sindbis virus against vesicular stomatitis virus (vsv) in a manner analogous to intrinsic interference with newcastle disease virus replication. interference in both systems (i) depends upon early expression of the inducing virus genome, (ii) shows similar kinetics of induction, (iii) does not involve interferon action, and (iv) appears to be manifest as an all-or-none effect. vsv can be added to the list of viruses blocked by intrinsic interference. ... | 1974 | 4365326 |
| reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (indiana serotype), chandipura, vesicular stomatitis (new jersey serotype), and cocal viruses. | rna transcriptase activities have been reconstituted from fractionated components of four rhabdoviruses: vesicular stomatitis virus (vsv) (indiana serotype), cocal, vsv (new jersey), and chandipura viruses. heterologous reconstitution of transcription has been observed between template and enzyme fractions of vsv indiana and cocal viruses but not to any significant extent between components of the other two viruses with vsv indiana template or enzyme. homologous reconstitution of infectivity has ... | 1974 | 4365327 |
| complementation and phenotypic stabilization of vesicular stomatitis virus temperature-sensitive and thermolabile mutants by avian myeloblastosis virus. | 1974 | 4362994 | |
| polyadenylation of vesicular stomatitis virus mrna. | vesicular stomatitis virus (vsv) mrna isolated from infected cell polysomes contains polyadenylic acid [poly(a)] sequences. detergent-activated purified virions in vitro can transcribe complementary rna, which has sedimentation properties similar to mrna, and this rna also contains poly(a) sequences. digestion of virion rna with u2 rnase under conditions where hydrolysis is specific for purine linkages leaves no sequences of polyuridylic acid corresponding in length to the poly(a) on the transcr ... | 1974 | 4363251 |
| carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus. | 1974 | 4366497 | |
| serological characterization of the three major proteins of vesicular stomatitis virus. | the three major proteins of vesicular stomatitis virus-indiana, glycoprotein (g), nucleoprotein (n), and membrane protein (m), were isolated and characterized by means of specific monocomponent antisera. g, n, and m proteins are distinct, nonrelated antigens with specific serological properties. the g protein is the only antigen inducing the formation of virus-neutralizing antibodies and was shown to confer immunity to mice. specific complement-fixing and precipitating activity was demonstrated ... | 1974 | 4365717 |
| [karyological study of l cells sensitive and resistant to vesicular stomatitis virus]. | 1974 | 4366340 | |
| ozone effect on respiratory deposition of vesicular stomatitis virus aerosols. | 1974 | 4361388 | |
| translation of vesicular stomatitis messenger rna by extracts from mammalian and plant cells. | rna was isolated from polyribosomes of vesicular stomatitis virus (vsv)-infected cells and tested for its ability to direct protein synthesis in extracts of animal and plant cells. in cell-free, non-preincubated extracts of rabbit reticulocytes, the 28s vsv rna stimulated synthesis of a protein the size of the vesicular stomatitis virus l protein whereas the 13 to 15s rna directed synthesis of the vsv m, n, ns, and possibly g proteins. in wheat germ extracts, 13 to 15s rna also directed synthesi ... | 1974 | 4359431 |
| rna polymerase activity and poly(a) synthesizing activity in defective t particles of vesicular stomatitis virus. | 1974 | 4362548 | |
| inhibitor of vesicular stomatitis virus transcriptase in purified virions. | 1974 | 4361996 | |
| pseudotypes of vesicular stomatitis virus determined by exogenous and endogenous avian rna tumor viruses. | 1974 | 4362025 | |
| cyclic production of vesicular stomatitis virus caused by defective interfering particles. | 1974 | 4361583 | |
| effect of ionic strength on the binding of sindbis virus to chick cells. | sindbis virus can adsorb to chicken embryo fibroblasts in two different ways. "loosely" bound virus can be washed off the cell with buffers of ionic strength 0.2 or greater, whereas "tightly" bound virus remains attached under these conditions. when sindbis virus is adsorbed to chick cells at 4 c from a buffer of ionic strength 0.17, 40 to 50% of the adsorbed virus is loosely bound, the remainder tightly bound. infection of chick cells by sindbis virus has only small effects on the total amount ... | 1974 | 4363248 |
| effect of interferon upon the primary and secondary transcription of vesicular stomatitis and influenza viruses. | the synthesis of viral complementary rna by vesicular stomatitis virus in permissive cells can be distinguished operationally into two phases, primary transcription, which can be observed in cycloheximide treated cells, and secondary transcription, which represents the further synthesis of viral complementary rna obtained in untreated cells. pretreatment of mouse l cells or chicken embryo fibroblasts with the homologous interferon and, for chicken embryo fibroblast cells, poly(ri):poly(rc), inhi ... | 1974 | 4372392 |
| envelope proteins of vesicular stomatitis virus: effect of temperature-sensitive mutations in complementation groups iii and v. | all five major viral proteins were synthesized in chicken embryo cells infected with vesicular stomatitis virus temperature-sensitive (ts) mutants of complementation groups iii and v and maintained at the nonpermissive temperature. the distribution of these proteins among cytoplasmic cellular fractions separated on discontinuous sucrose gradients was identical for wild-type and tsiii-infected cells. strikingly different patterns were observed for the g protein in gradients from cells infected by ... | 1974 | 4372395 |
| newcastle disease virus infection of l cells. | newcastle disease virus (ndv) california strain reportedly grows poorly in l cells but replicates very well in chicken embryo cells. ndv-infected l cell cultures show a characteristic virus growth curve with respect to uridine incorporation, but plaque assays of the virus produced 24 h postinfection (pi) show no infectious particles when assayed on l cell monolayers and only a very low titer on chick cell monolayers. plasma membranes isolated and purified from infected l cells 8 h pi contain all ... | 1974 | 4836600 |
| in vivo synthesis of rna by vesicular stomatitis virus and its mutants. | 1974 | 4372356 | |
| pathogenicity and immunogenicity for mice of temperature-sensitive mutants of vesicular stomatitis virus. | temperature-sensitive (ts) mutants of vesicular stomatitis (vs) virus were tested for their pathogenicity and immunogenicity in weanling mice. compared with the wild-type virus (ts(+)), ts mutants representing genetic complementation groups i, ii, and iv were considerably less pathogenic for mice infected by the intracerebral route and caused few deaths after intranasal inoculation. mice were completely resistant to ts(+) and ts mutants by the intraperitoneal route. resistance to intracerebral c ... | 1974 | 16558079 |
| inhibition of oncornavirus functions by 2'-azido polynucleotides. | the 2'-azido analogs of poly(u) and poly(c), poly(duz) [poly(2'-azido-2'-deoxyuridylic acid)], and poly-(dcz [poly(2'-azido-2'-deoxycytidylic acid)], were found to inhibit the rna-directed dna polymerase (reverse transcriptase) activity of murine leukemia (moloney, rauscher) and sarcoma (moloney) virus, and feline leukemia (theilen) and sarcoma (gardner) virus, while under the same conditions the unsubstituted parent compounds failed to do so. in addition, poly(duz) and poly(dcz) inhibited the r ... | 1975 | 47174 |
| influence of interferon on the synthesis of virus particles in oncornavirus carrier cell lines. iii. survey of effects on a-, b- and c-type oncornaviruses. | the effect of interferon on the synthesis and release of a-, b- and c-type viruses by oncornavirus carrier lines was studied. murine cell lines were selected which carry either of these viruses and are sensitive to the antiviral effect of interferon, as measured by inhibition of vesicular stomatitis virus. release of c-type virus was found to be highly sensitive. release of b-type virus, on the contrary, was only marginally inhibited. synthesis of intracisternal a-type particles was finally not ... | 1975 | 50292 |
| immunologically specific production of interferon in cultures of rabbit blood lymphocytes: association with in vitro tests for cell-mediated immunity. | lymphocytes of animals with delayed hypersensitivity produce mediators of cellular immunity when challenged in vitro with specific antigen. among these are macrophage migration inhibitory factor (mif) and interferon (if). nonspecific mitogens also induce the production of these lymphokines. in the following study leukocytes and column-purified lymphocytes of the same peripheral blood sample from tuberculin (purified protein derivatives [ppd])-sensitive rabbits were concurrently cultured in mediu ... | 1975 | 53204 |
| virus envelope markers in mammalian tropism of avian rna tumor viruses. | pseudotypes of vesicular stomatitis virus were prepared with avian sarcoma viruses and avian leukemia viruses representing five different subgroups. these pseudotypes display a host range restricted to that of the avian tumor virus when assayed on avian cells and are neutralized by subgroup-specific antisera. the efficiency of penetration of mammalian cells was assayed by using these vesicular stomatitis virus pseudotypes. pseudotypes of avian tumor viruses belonging to subgroup d and of b77 vir ... | 1975 | 163337 |
| persistence of vesicular stomatitis virus in interferon-treated cell cultures. | 1975 | 163524 | |
| rna synthesis by temperature-sensitive mutants of vesicular stomatitis virus, new jersey serotype. | 1975 | 163530 | |
| porcine parvovirus: frequency of naturally occurring transplacental infection and viral contamination of fetal porcine kidney cell cultures. | the frequency of naturally occurring transplacental infection of swine with porcine parvovirus (ppv) and one of the possible consequences of such infection--the presence of ppv in cell cultures prepared from fetal tissues--were investigated. transplacental infection was indicated by the presence of high titers of hemagglutination inhibiting (hi) antibody for ppv in serums of 0-day-old, hysterectomy-derived, colostrum-deprived pigs of 3 of 82 litters. all letters were farm-raised dams. moreover, ... | 1975 | 163603 |
| cell-free synthesis of vesicular stomatitis virus proteins: translation of membrane-bound polyribosomal mrnas. | 1975 | 163768 | |
| vesicular stomatitis virus (measles) pseudotypes: tool for demonstrating defective measles infections. | mixed infection of vero cells with measles and vesicular stomatitis viruses gives rise to phenotypically mixed infections virus particles possessing the properties of pseudotypes. similar results were obtained when vesicular stomatitis virus was used to superinfect a defective measles system. | 1975 | 61189 |
| cell killing by viruses. ii. cell killing by vesicular stomatitis virus: a requirement for virion-derived transcription. | 1975 | 163045 | |
| progeny resulting from complementation between mutants of vesicular stomatitis virus. | the complementation properties of the virus progeny released from cells mixedly infected with mutants of vesicular stomatitis virus belonging to four different complementation groups have been examined. the group iv mutant, tsw16b, was tested in combinations with three group i mutants (tsw4, tsw28, and tsg11), one group ii mutant (tsg22), and one group iii mutant (tsw29). virus stocks were grown from isolated plaques appearing on the cell monolayers used to assay the mixed infection yields and t ... | 1975 | 163131 |
| restitution of infectivity to spikeless vesicular stomatitis virus by solubilized viral components. | noninfectious spikeless particles have been obtained from vesicular stomatitis virus (vsv, indiana serotype) by bromelain or pronase treatment. they lack the viral glycoprotein (g) but contain all the other viral components (rna, lipid, and other structural proteins). triton-solubilized vsv-indiana glycoprotein preparations, containing the viral g protein as well as lipids (including phospholipids), have been extracted from whole virus preparations, freed from the majority of the detergent, and ... | 1975 | 166210 |
| virus development in enucleate cells: echovirus, poliovirus, pseudorabies virus, reovirus, respiratory syncytial virus and semliki forest virus. | a group of rna viruses, echovirus, poliovirus, reovirus, respiratory syncytial virus and semliki forest virus have been examined for ability to grow in enucleate african green monkey kidney (bsci) cells. semliki forest virus produced an almost normal yield of virus but poliovirus, echovirus, reovirus and respiratory syncytial virus, although showing clear evidence of virus replication when compared with a nuclear dna virus (pseudorabies virus) gave much lower yields than those from nucleate cell ... | 1975 | 163289 |
| screening procedure for complementation-dependent mutants of vesicular stomatitis virus. | to isolate new types of vesicular stomatitis virus (vsv) mutants, a four-stage screen was developed which identifies and characterizes mutants capable of complementing the defect in the vsv temperature-sensitive mutant tsg11. two types of mutants of vsv, indiana serotype, have been found by using the screen; they are new temperature-sensitive mutants which are, of necessity, not in complementation group i and mutants which do not produce plaques under conditions of single infection at 31 c (the ... | 1975 | 163345 |
| analysis of uridine incorporation in chicken embryo cells infected by vesicular stomatitis virus and its temperature-sensitive mutants: uridine transport. | the shut-off of rna synthesis in chicken embryo cells, after infection with vesicular stomatitis virus, is partially due to a reduced capacity of the infected cells to transport uridine. permeability to uridine decreases exponentially after infection. this loss of ability to transport uridine may be caused either by structural components of the input virions or may result from the expression of the viral gene products. in the latter case, only minor levels of viral transcription is sufficient to ... | 1975 | 163348 |
| scanning electron microscopic studies of virus-infected cells. i. cytopathic effects and maturation of vesicular stomatitis virus in l2 cells. | l2 cells infected with vesicular stomatitis virus under single-cycle conditions have been studied by scanning electron microscopy after preparation by the critical point drying technique. three dimensional images of intact cells show bullet-shaped vesicular stomatitis virus virions budding singly and in radiating clusters both from the plasma membrane between cellular microvilli and from the sides of microvilli. virus-induced cytopathic effects observed by scanning electron microscopy include in ... | 1975 | 163360 |
| action of interferon in enucleated cells. | interferon induces protection of enucleated bsc-1 cells against infectious vesicular stomatitis virus production if cells are treated before, but not after, enucleation. | 1975 | 163365 |
| 13-c nuclear magnetic resonance studies on the lipid organization in enveloped virions (vesicular stomatitis virus). | 13-c nuclear magnetic resonance (nmr) studies are described regarding the lipid organization in the envelope of the vesicular stomatitis virion. the fatty acid chains (oleic acid) and the choline moiety of the 3-sn-phosphatidylcholine and spingomyelin have been labeled specifically with 13-c by growing the virions in prelabeled host cells (bhk 21 cells). the results suggest that 130c nmr spectroscopy is a very feasible method for the study of natural membranes provided the isotope is highly enri ... | 1975 | 167809 |
| inactivation of viruses and bacteria by ozone, with and without sonication. | selected organisms with public health significance were placed in a reaction chamber for treatment by ozonation, by ozonation and sonication, by sonication, or by sonication during oxygenation. vesicular stomatitis virus, encephalomyocarditis virus, gdvii virus, staphylococcus aureus, pseudomonas fluorescens, salmonella typhimurium, enteropathogenic escherichia coli, vibrio cholerae, and shigella flexneri were inactivated by treatment with ozone. when microorganisms were suspended in phosphate-b ... | 1975 | 163616 |
| messenger rna species synthesized in vitro by the virion-associated rna polymerase of vesicular stomatitis virus. | the virion-associated rna-dependent rna polymerase of vesicular stomatitis virus (vsv) synthesizes in vitro two size classes of rna products similar to those observed in vsv-infected cells. one rna product sediments at 31s with an approximate molecular weight of 2.1 x 106. the smaller products consist of at least three classes of rna sedimenting at 17s, 14.5s, and 12s with molecular weights of 0.7 x 106, 0.52 x 106, and 0.37 x 106, respectively. hybridization experiments show that both the 31s a ... | 1975 | 163702 |
| characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the bgm/mv cell line. | serological methods of mixed agglutination and indirect immunofluorescence showed the bgm/mv cell line to possess monkey antigens. as a means of further characterizing the species constitution of the bgm/mv cell line, the species specificity of viral-induced interferon from these cells, as well as the response of these cells to exogenous interferons, was determined. low titers of spontaneously elaborated interferon capable of protecting monkey but not mouse cells were detected in bgm/mv culture ... | 1975 | 163789 |
| translation of individual species of vesicular stomatitis viral mrna. | vesicular stomatitis virus mrnas from three of the four bands fractionated by polyacrylamide gel electrophoresis in 99% formamide have been eluted from gels and translated in the krebs ii ascites cell-free system. band 2 mrna (0.7 times 10-6 daltons) directed the synthesis of the protein moiety of the glycoprotein (g), and band 3 (0.55 times 10-6 daltons) coded for the nucleocapsid (n) protein. band 4 mrna (o.28 times 10-6 daltons) directed the synthesis of the ns and matrix (m) proteins. the au ... | 1975 | 163911 |
| translation and identification of the viral mrna species isolated from subcellular fractions of vesicular stomatitis virus-infected cells. | the cytoplasm of vesicular stomatitis virus (vsv)-infected bhk cells has been separated into a fraction containing the membrane-bound polysomes and the remaining supernatant fraction. total poly(a)-containing rna was isolated from each fraction and purified. a 17s class of vsv mrna was found associated almost exclusively with the membrane-bound polysomes, whereas 14,5s and 12s rnas were found mostly in the postmembrane cytoplasmic supernatant. poly(a)-containing vsv rna synthesized in vitro by p ... | 1975 | 163912 |
| methylation-dependent translation of viral messenger rnas in vitro. | methylated reovirus and vesicular stomatitis virus mrnas, synthesized in vitro in the presence of s-adenosylmethionine by the virion-associated polymerases (rna nucleotidyltransferases, ec 2.7.7.6), stimulate protein synthesis by wehat germ extracts to a greater extent than unmethylated mrnas. addition of s-adenosylmethionine to a cell-free extract programmed with unmethylated mrna stimulates protein synthesis and results in methylation of the mrna. an inhibitor of mrna methylation. s-adenosylho ... | 1975 | 165487 |
| physical properties of new jersey serotype of vesicular stomatitis virus and its defective particles. | the wild-type new jersey serotype of vesicular stomatitis virus generated two types of defective interfering t-particles. the physical properties of these particles and the wild-type virion were determined by laser light scattering spectroscopy, sedimentation measurements, and electron microscopy. | 1975 | 165488 |
| mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity. | vesicular stomatitis virus propagated in and released from aedes albopictus cells had the normal complement of viral proteins; the glycoprotein contained carbohydrate but no sialic acid. these virions had markedly reduced hemagglutinating activity and exhibited a very high ratio of physical particles to infectious virus. in vitro sialylation of vesicular stomatitis virions grown in mosquito cells resulted in a 100-fold increase in both infectivity and hemagglutination titers to levels approachin ... | 1975 | 163913 |
| nongenetic complementation of group v temperature-sensitive mutants of vesicular stomatitis virus by uv-irradiated virus. | cells infected with temperature-sensitive (ts) mutants of complementation group v of vesicular stomatitis virus (vsv) give an enhanced yield at nonpermissive temperature when co-infected or superinfected with uv-irradiated virus. virions produced in these mixed infections are temperature sensitive and do not complement ts v45. rescue of group v mutants is multiplicity dependent. it can occur in the presence of cycloheximide; kinetics of rescue are similar in the absence or in the presence of the ... | 1975 | 163919 |
| sub-cellular localization of vesicular stomatitis virus messenger rnas. | 1975 | 164182 | |
| site of synthesis of membrane and nonmembrane proteins of vesicular stomatitis virus. | upon infection of chinese hamster ovary cells (cho), vesicular stomatitis (vsv) virus synthesizes two membrane proteins (the vsv glycoprotein and the vsv matrix or membrane (m) protein) and three nonmembrane proteins (the vsv nucleocapsid, the viral transcriptase, and an ns protein). we have used the vsv-infected cell as a model system for the study of the site of synthesis of these membrane and nonmembrane proteins. we have isolated vsv mrna from free polyribosomes, membrane-bound polyribosomes ... | 1975 | 169263 |
| host effect on vesicular stomatitis virus morphogenesis and "t" particle formation in reptilian, avian, and mammalian cell lines. | the morphogenesis of vesicular stomatitis virus (vsv) in three reptilian cell lines (turtle heart, gecko lung, and viper spleen) was studied by thin section electron microscopy. simultaneous growth studies were conducted in reptilian, chick embryo fibroblast, and bhk/21 cells to compare the yields of infectious vsv during serial passages. the mean length of gecko lung cell vsv measured from electron micrographs is statistically significantly shorter than that of vsv replicating in other reptilia ... | 1975 | 170198 |
| novel initiation of rna synthesis in vitro by vesicular stomatitis virus. | 1975 | 165428 | |
| selective blockage of initiation of host protein synthesis in rna-virus-infected cells. | poliovirus mrna and mrna transcribed from vesicular stomatitis virus and reovirus genomes efficiently direct protein synthesis in vivo under experimental conditions where the initiation of host protein synthesis is selectively blocked. the selective blockage of host peptide chain initiation after exposure to hypertonic medium indicates that the translation of viral mrna is more efficiently initiated than is the translation of host mrna. it further suggests that virus directed suppression of host ... | 1975 | 165500 |
| the 5' terminal structure of the methylated mrna synthesized in vitro by vesicular stomatitis virus. | the 5' terminal structure of the mrna synthesized in vitro by the virion-associated rna polymerase of vesicular stomatitis virus in the presence of s-adenosyl-l-methione consists of 7-methyl guanosine linked to 2'-o-methyl adenosine through a 5'-5' pyrophosphate bond as m7g(5')ppp(5')a-m-p ... the alpha and beta phosphated of gtp and alpha phosphate of atp are incorporated into the blocked 5' terminal structure. | 1975 | 165900 |
| methylated and blocked 5' termini in vesicular stomatitis virus in vivo mrnas. | methyl groups derived from 3h-methyl methionine were incorporated into vesicular stomatitis virus (vsv) mrnas isolated from infected cells. sequential degradation of the 12-18s viral mrna species with ribonuclease t2, penicillium nuclease, and alkaline phosphatase yielded a single 3h-labeled dinucleotide. a similar resistant 32p-labeled fragment was obtained by digesting vsv mrna uniformly labeled with 32p. this methylated and blocked oligomer was further cleaved with nucleotide pyrophosphatase, ... | 1975 | 165901 |
| inhibition of herpesvirus hominis replication by human interferon. | the effect of human interferon on single-cycle replication of herpesvirus hominis type 1 (hv-1) and vesicular stomatitis virus (vsv) in human foreskin fibroblast cultures (hff) was studied. after treatment of hff cultures with low concentrations (58 u) of human interferon, a variable but statistically significant inhibition of hv-1 was observed. at higher concentrations (greater than 95 u) yield reduction of hv in interferon-treated cultures approached that of vsv. preliminary data indicate that ... | 1975 | 166922 |
| efficacy of exogenous interferon treatment initiated after onset of multiplication of vesicular stomatitis virus in the brains of mice. | initiation of treatment with potent interferon preparations (6-4 x 10-6 units per dose) 4 days after intranasal inoculation of vsv (at a time when virus has already multiplied in the brains of most mice) resulted in a marked increase in mouse survival. in a series of 6 experiments only 11 to 18% of control mice survived whereas 52% of interferon treated mice survival. these results suggest the usefulness of exogenous interferon even when treatment is begun late in the course of an acute virus di ... | 1975 | 167120 |
| method of examining viral rna metabolism in cells in culture: metabolism of vesicular stomatitis virus rna. | a method is described for radioactively labeling viral rna and then quickly halting further incorporation of radioactive precursor into rna so that the fate of the labeled rna can be followed. small complementary rnas synthesized during vesicular stomatitis virus infection in the presence of cycloheximide do not metabolize to virion length molecules when protein synthesis inhibition is reversed. | 1975 | 171456 |
| both ns and l proteins are required for in vitro rna synthesis by vesicular stomatitis virus. | vesicular stomatitis virions, indiana serotype, were solubilized with high salt solubilizer and separated by ultracentrifugation into a supernatant fraction containing l, g, ns, and m proteins and pellet fraction containing the rna complexed with n protein. ns protein was purified from the supernatnat fluid by sequential chromatography on phosphocellulose and diethylaminoethyl cellulose columns. the purified ns protein was assayed in a standard transcription system in combination with purified l ... | 1975 | 167189 |
| rubella and rheumatoid arthritis: hyaluronic acid and susceptibility of cultured rheumatoid synovial cells to viruses. | synovial cell lines were established from patients with rheumatoid arthritis (ra) and from normal human embryos. high levels of hyaluronic acid (ha) were produced by some ra cell lines, some of which were partially or completely resistant to infection with newcastle disease virus (ndv), vesicular stomatitis virus (vsv), and rubella virus (rv). normal fetal synovial cells lines were susceptible to ndv, vsv, and rv. infection with virus became possible after treatment of ra cells with hyaluronidas ... | 1975 | 167379 |
| association of vesicular stomatitis virus glycoprotein with virion membrane: characterization of the lipophilic tail fragment. | the proteolytic enzyme, thermolysin, degraded the external segment of the membrane glycoprotein of intact vesicular stomatitis (vs) virions but left behind a small nonglycosylated fragment, presumably embedded in the virion membrane. other proteases generated membrane-associated glycoprotein fragments differing somewhat in molecular weight. the thermolysin-resistant, virion-associated fragment, which can be selectively solubilized by either triton x-100 or chloroform/methanol, has a molecular we ... | 1975 | 168400 |
| further evidence for the existence of a viral envelope protein defect in the bryan high-titer strain of rous sarcoma virus. | electron microscopy observations of purified bryan high-titer rous sarcoma virus (bh rsv) using the freeze-drying technique showed that progeny made in the absence of a helper virus lacked visible surface projections or spikes. phenotypic mixing experiments employing bh rsv and a thermolabile mutant of vesicular stomatitis virus, tl 17, yielded no evidence of pseudotype formation. since tl 17 is known to be defective for an envelope glycoprotein, the lack of successful phenotypic mixing with bh ... | 1975 | 168409 |
| association of ribosomes with intracellular vesicular stomatitis virus particles. | ribosomes are observed intimately associated with nucleocapsids of vesicular, stomatitis virus, especially those that line structures that are either cytoplasmic vesicles or invaginations of the plasma membrane. | 1975 | 168410 |
| effect of ascorbic acid, sodium salicylate, and caffeine on the serum interferon level in response to viral infection. | the ability of ascorbic acid, sodium salicylate, and caffeine to alter the circulating serum level of interferon was investigated in mice. the animals were singly injected subcutaneously with one of the compounds, 4-8 h later again singly injected intraperitoneally with poly i:c, and bled 6-8 h afterward. the sera from the mice were assayed for interferon titer by the use of the plaque inhibition method utilizing vesicular stomatitis virus. ascorbic acid, sodium salicylate, and caffeine increase ... | 1975 | 168598 |
| virus-specific changes in mouse cells inoculated with a strain of human cytomegalovirus. | cytopathic changes and virus-specific antigens developed in, then disappeared from, mouse fibroblasts infected by a strain of human cytomegalovirus (cmv), but their disappearance was delayed in cells treated with idoxuridine prior to infection. the replication of vesicular stomatitis virus and herpes simplex virus was restricted in human cmv-infected mouse cells as long as human cmv-specific antigens were present. virus-specific antigens could be induced by treatment with idoxuridine or arginine ... | 1975 | 168741 |
| phenotypically mixed vesicular stomatitis virus particles produced in human tumor cell lines. | 1975 | 169045 | |
| phenotypic mixing between vesicular stomatitis virus and avian rna tumor viruses. | 1975 | 169046 | |
| conditional lethal mutants of vesicular stomatitis virus. | 1975 | 169102 | |
| effect to tween 80 and aquasol a on virus plaque formation. | the enhancement of vesicular stomatitis virus plaques on human embryonic lung cells in the presence of tween 80 or aquasol a was studied to determine the optimal conditions for the enhancement. enhanced numbers and sizes of vesicular stomatitis virus plaques occurred when aquasol a or tween 80 was added to the cell culture 30 min before virus adsorption but not when added after adsorption. these substances did not have a direct effect on the virus and did not have an effect on virus adsorption o ... | 1975 | 170202 |
| heterogneeous 5'-terminal structures occur on vesicular stomatitis virus mrnas. | four alternative structures occur at the 5' ends of vesicular stomatitis virus mrnas synthesized in infected cells and are separated conveniently by a technique described here. sixty-five to seventy per cent of the mrna molecules have the 5' end structure m7g5'ppp5'(m)ampap and about 20% have a more highly modified structure m7g5'ppp5'(m)ampmampcp. the base of the first adenosine in each sequence is methylated in about one-half of the ends of each type and kinetic experiments suggest that the la ... | 1975 | 170280 |
| lethal synergy between sarcoma-180/tg cells and vesicular stomatitis virus in mice. | an interaction between sarcoma-180/tg cells and vesicular stomatitis virus in adult mice resulted in the rapid onset of extensive mortality. this interaction, termed lethal synergy, occurred only at early stages of ascites induction in animals with no prior virus contact. a significant sparing effect conferred by the serotonin antagonist dibenamine was reversed by the administration of serotonin. the cause of death was not determined, but a mechanism involving hypersensitivity is indicated. | 1975 | 174141 |
| inhibition of vesicular stomatitis virus replication by poly(2'-fluoro-2'-deoxyuridylic acid). | poly(2'-fluoro-2'-deoxyuridylic acid) is known to be an effective inhibitor of the deoxyribonucleic acid polymerase found within the oncornaviruses. this synthetic polynucleotide was found to inhibit the replication of vesicular stomatitis virus in mouse l cells. the polymer was shown to be capable of inhibiting the viral ribonucleic acid (rna)-dependent rna polymerase, and it is proposed that this is the mechanism of antiviral activity. the following observations support this viewpoint: (i) the ... | 1975 | 174488 |
| polykaryocytosis induced by vesicular stomatitis virus infection in bhk-21 cells. | cytopathological effects by vesicular stomatitis virus (vsv) infection were studied in several cell lines. marked polykaryocyte formation was observed in monolayers of certain strains of bhk-21 cells infected with vsv. the bhk-21-kb cells were found to be the most susceptible to virus-induced cell fusion. this type of cell fusion was related to intracellular growth of the viruses, and strong cytolytic changes were found to occur following the development of large multinucleated giant cells. the ... | 1975 | 174526 |