Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| enzootic vesicular stomatitis new jersey type in an insular feral swine population. | free-ranging feral swine from ossabaw island, chatham county, georgia, were serially bled and tested for vesicular stomatitis new jersey type serum neutralizing antibody to determine the intensity and progression of annual vesicular stomatitis activity. from november 21, 1981 to october 11, 1982, and from march 15, 1983 to october 14, 1983, 307 and 340 swine were sampled, respectively. seroconversions were initially detected during the first week of june and continued into september in both 1982 ... | 1985 | 2996331 |
| characterization of new jersey vesicular stomatitis virus isolates from horses and black flies during the 1982 outbreak in colorado. | vesicular stomatitis viruses isolated from horses, afflicted during the recent outbreak in the western united states, and from black flies (simuliidae) were characterized with respect to the homology of their genomic rnas and the mobility of their proteins in polyacrylamide gels. all the isolates were very similar, if not identical, with respect to these two parameters. when the black fly isolate was compared to other vsv isolates, this virus appeared to belong in the hazelhurst subgroup of the ... | 1985 | 2997995 |
| gangliosides in early interactions between vesicular stomatitis virus and cer cells. | in the present report an attempt was made to elucidate the role of gangliosides in early interactions between vesicular stomatitis virus (vsv) and cer cells. research was carried out to test the ability of gangliosides from mammal brains and from cer cells to inhibit viral attachment to susceptible cells. the incubation of vsv in the presence of gangliosides decreased the subsequent infection of cer cells by the virus. when similar experiments were performed with gangliosides inserted in liposom ... | 1985 | 2999565 |
| recent vesicular stomatitis virus infection detected by immunoglobulin m antibody capture enzyme-linked immunosorbent assay. | we developed an enzyme-linked immunosorbent assay (elisa) that was capable of detecting immunoglobulin m (igm) antibody to vesicular stomatitis virus (vsv) in the sera of experimentally and naturally infected cattle and horses. the detection of igm in the sera of these animals permitted an estimate of the recency of infection by vsv serotype new jersey. a vsv serotype new jersey epizootic strain isolated from a horse and passed once in an aedes albopictus cell line was used to infect a horse and ... | 1985 | 3001132 |
| complete nucleotide sequence of the matrix protein mrna of vesicular stomatitis virus (new jersey serotype). | the complete nucleotide sequence of the mrna of the matrix (m) protein of vesicular stomatitis virus [new jersey serotype, vsv(nj)] was derived from a cdna clone and mrna. the mrna is 758 nucleotides long (excluding polyadenylic acid) and encodes a protein of 229 amino acids. the predicted amino acid sequence was compared with that of the corresponding protein of indiana serotype [vsv(ind)] and a fish rhabdovirus, spring viremia of carp virus (svcv). an amino acid identity of 62% was found betwe ... | 1986 | 3006343 |
| primary antibody responses to a well-defined and unique hapten are not enhanced by preimmunization with carrier: analysis in a viral model. | we used a viral model to reexamine classical experiments showing that mice previously primed with a "carrier" molecule alone and then challenged with the carrier-hapten conjugate exhibited an enhanced antihapten antibody response. mice were primed with live or uv-inactivated vesicular stomatitis virus (vsv) indiana (ind) serotype with or without complete freund's adjuvant. after challenge with vsv new jersey (nj), these mice developed a secondary-type igg response, measured by antibody binding i ... | 1986 | 3010314 |
| recognition of cloned vesicular stomatitis virus internal and external gene products by cytotoxic t lymphocytes. | it has generally been assumed that most if not all ctl specific for vesicular stomatitis virus (vsv)-infected cells recognize the viral glycoprotein (g), an integral membrane protein abundantly expressed on infected cell surfaces. using recombinant vaccinia viruses containing copies of cloned vsv genes to examine ctl recognition of vsv, we have confirmed that g is recognized by vsv-specific ctl. more interestingly, however, we have also found that nucleocapsid protein (n), an internal virion pro ... | 1986 | 3011949 |
| antibodies to vesicular stomatitis new jersey type virus in a population of white-tailed deer. | 1986 | 3012128 | |
| conservation of potential phosphorylation sites in the ns proteins of the new jersey and indiana serotypes of vesicular stomatitis virus. | a full length cdna copy of the ns mrna of the missouri strain (hazelhurst subtype, new jersey serotype) of vesicular stomatitis virus (vsv) has been cloned and sequenced. the mrna is 856 nucleotides long (excluding polyadenylic acid) and encodes a protein of 274 amino acids (mol. wt. 31 000). comparison with the ns gene of the ogden strain (concan subtype, new jersey serotype) showed 15% difference at the nucleotide level and 10% difference at the amino acid level; the majority of the changes we ... | 1986 | 3014048 |
| antibodies to vesicular stomatitis virus in populations of feral swine in the united states. | from 1979 to 1985, 941 feral swine (sus scrofa) from 53 locations in 15 states were serologically tested for antibodies to vesicular stomatitis virus (vsv). antibodies to new jersey serotype vsv were present in 75 swine from five locations in arkansas, florida, georgia, and louisiana. within these populations, antibody prevalences ranged from 10 to 100%. no antibodies to indiana serotype were detected. | 1986 | 3016348 |
| an enzyme-linked immunosorbent assay for the detection of bovine antibodies to vesicular stomatitis virus. | an indirect enzyme-linked immunosorbent assay (elisa) was developed to detect bovine antibody to vesicular stomatitis virus (vsv). serum samples from cows experimentally infected with the new jersey serotype of vsv (vsv-nj) were assayed by the elisa and serum-neutralization (sn) assay. the elisa was as sensitive as the sn assay in detecting bovine antibody to vsv. the correlation between sn titers and elisa values at absorbance at 405 nm was statistically significant. the elisa was not specific ... | 1986 | 3017162 |
| in vitro exposure of preimplantation bovine embryos to vesicular stomatitis virus. | vesicular stomatitis virus new jersey serotype (vsv-nj) adhered to 14 of 20 zonae pellucidae intact (zp-i) bovine embryos exposed in vitro. the vsv-nj-exposed zp-i bovine embryos were washed by a single- or multiple-pipette procedure. the multiple-pipette washing procedure was more efficient in removing unattached virus than the single-pipette procedure, but neither washing procedure was effective in consistently removing attached virus from zp-i embryos. the virus plaque assay with vero-maru ce ... | 1986 | 3020081 |
| defective interfering particles of vsvnj (ogden), generated by heat treatment, contain multiple internal genomic deletions. | defective interfering (di) particles have been isolated from a heat-resistant strain of the new jersey (ogden) serotype of vesicular stomatitis virus (vsv). most of these di particles contain various portions of all five cistrons of vsv. the two largest di particles, nj-121 and nj-pg2, represent approximately 60% of the standard virus genome and contain both the positive and negative strand leader rna templates. these two di particles are transcriptionally active and synthesize both the positive ... | 1986 | 3022478 |
| genome rna terminus conservation and diversity among vesiculoviruses. | sequence analysis of the rna genome termini of various vesiculovirus standard and defective interfering (di) particles demonstrated that some virus regulatory sequences and domains of virus n protein are highly conserved while others show considerable divergence. clearly, distinct rna signal sequences and protein-coding regions of these virus genomes have quite different evolutionary pressures or constraints. terminal regions of di-particle rna genomes of these viruses were found to possess self ... | 1987 | 3023698 |
| identification of a domain within the phosphoprotein of vesicular stomatitis virus that is essential for transcription in vitro. | a full-length cdna copy of the phosphoprotein (ns) mrna of vesicular stomatitis virus (new jersey serotype) was inserted into pgem4 vector downstream of the promoter for bacteriophage sp6 rna polymerase. transcription of the cdna in vitro resulted in the synthesis of ns mrna, which was subsequently translated into ns protein in a cell-free rabbit reticulocyte system. the biological activity of the expressed ns protein was demonstrated by in vitro synthesis of mrna by transcription-reconstitution ... | 1986 | 3024153 |
| characterization of the mutations responsible for the electrophoretic mobility differences in the ns proteins of vesicular stomatitis virus new jersey complementation group e mutants. | temperature-sensitive (ts) mutants of vesicular stomatitis virus, new jersey serotype, classified in complementation group e contain lesions in the ns gene, which manifest as marked electrophoretic mobility differences of the mutant ns proteins in sds-polyacrylamide gels. we have cloned full-length cdna copies of the mutant ns mrnas, and have determined their nucleotide sequences. tse1 and tse3 had single nucleotide changes, and tse2 had two nucleotide changes, compared to the wild-type ns gene. ... | 1986 | 3025344 |
| epizootic vesicular stomatitis in colorado, 1982: epidemiologic and entomologic studies. | an epizootic of vesicular stomatitis (vs) caused by the new jersey serotype of vs virus affected livestock and humans in 14 western states in 1982-1983. epidemiological observations were made on at least 10% of the cattle in 4 dairy herds that were located in the vicinity of grand junction, colorado. high rates of neutralizing antibody to the new jersey serotype were seen in all cattle regardless of whether livestock in the dairy had clinical vs or a decrease in mild production. antibody titers ... | 1987 | 3028191 |
| epizootic vesicular stomatitis in colorado, 1982: infection in occupational risk groups. | in 1982-1983, an epizootic of vesicular stomatitis occurred in the western united states. veterinarians, research workers, and regulatory personnel who were exposed to vesicular stomatitis virus were examined for patterns of human infection and prevalence of vesicular stomatitis new jersey serotype neutralizing antibody. insight into the mechanism of transmission was sought by comparing activities of antibody-positive and antibody-negative persons. a statistically significant risk factor was a h ... | 1987 | 3028192 |
| molecular epizootiology and evolution of vesicular stomatitis virus new jersey. | vesicular stomatitis virus (vsv) has been shown previously to be capable of undergoing rapid mutational change during sequential experimental infections in various tissue culture cell systems (j. holland, k. spindler, f. horodyski, e. grabau, s. nichol, and s. vandepol, science 215:1577-1585, 1982). the present study was undertaken to determine the degree of genetic diversity and evolution of the virus under natural infection conditions and to gain insight into the epizootiology of the disease. ... | 1987 | 3029397 |
| sequences of chandipura virus n and ns genes: evidence for high mutability of the ns gene within vesiculoviruses. | the nucleotide sequence of the 3' end of the genome of chandipura (chp) virus, including the complete sequences of the nucleocapsid (n) and phosphoprotein (ns) genes was determined, principally from cloned cdnas of the n and ns mrnas. the ns mrna of chp virus is 908 bases in length and encodes a protein of 293 amino acids. comparison of the chp virus ns protein sequence with those of vesicular stomatitis virus of the new jersey serotype (vsv (nj)) and of the indiana serotype (vsv (ind] revealed ... | 1987 | 3029973 |
| antibodies to vesicular stomatitis new jersey type virus in wild and domestic sentinel swine. | wild sentinel swine on ossabaw island, chatham county, georgia, were serially bled and tested for vesicular stomatitis new jersey type virus neutralizing antibody to determine the intensity, distribution, and progression of annual viral activity. from march through september, 1984 and 1985, 112 and 226 juvenile (less than 8 months) swine, respectively, were sampled. seroconversions initially were detected on may 7, 1984 and may 18, 1985. incidence of seroconversion in wild swine reached 32% duri ... | 1987 | 3034056 |
| natural infection of humans, animals, and phlebotomine sand flies with the alagoas serotype of vesicular stomatitis virus in colombia. | five isolations of the alagoas serotype of vesicular stomatitis virus (rhabdoviridae: vesiculovirus) were made from naturally infected phlebotomine sand flies (lutzomyia spp.) collected in colombia. these are the first isolations of alagoas virus from an arthropod. replication of the virus occurred in laboratory-reared sand flies (lutzomyia longipalpis) after inoculation. bite and transovarial transmission of the virus was also demonstrated in experimentally infected sand flies. alagoas virus ne ... | 1987 | 3034087 |
| epizootic vesicular stomatitis in colorado, 1982: some observations on the possible role of wildlife populations in an enzootic maintenance cycle. | sera obtained from wild ungulates, carnivores, and rodents in colorado were tested for neutralizing (n) antibody against vesicular stomatitis, new jersey serotype (vsnj), virus to determine their involvement in the 1982 colorado vsnj epizootic in domestic animals. viremic and n antibody responses of two local rodent species to a 1982 colorado isolate of vsnj were determined in the laboratory. the rodents produced only weak viremias, but all developed n antibody. n antibody prevalences for vsnj i ... | 1987 | 3035241 |
| the fates of undermethylated mrna cap structures of vesicular stomatitis virus (new jersey) during in vitro transcription. | a dual label pulse-chase analysis employing s-adenosyl-l-[methyl-3h]methionine and [beta-32p]gtp has been devised to identify precursors to the cap structure 7mg(5')ppp(5')am present on the 5'-termini of vesicular stomatitis virus mrnas. both monomethylated cap structures, 7mg(5')ppp(5')a and g(5')ppp(5')am, have been detected in vitro in new jersey serotype reactions containing suboptimal concentrations of s-adenosyl-l-methionine. the simultaneous chasing of both radiolabeled substrates allowed ... | 1987 | 3039729 |
| homotypic and heterotypic exclusion of vesicular stomatitis virus replication by high levels of recombinant polymerase protein l. | the recombinant polymerase protein l of vesicular stomatitis virus (vsv) expressed in cos cells is able to transcribe and replicate the viral genome, resulting in complementation of temperature-sensitive polymerase mutants of vsv at the restrictive temperature (m. schubert, g. g. harmison, c. d. richardson, and e. meier, proc. natl. acad. sci. usa 82:7984-7988, 1985). here we report that the efficiency of complementation is dependent on the level of l protein expression. unexpectedly, only cells ... | 1987 | 3041035 |
| malpais spring virus: a new vesiculovirus from mosquitoes collected in new mexico and evidence of infected indigenous and exotic ungulates. | two virus isolates, 1 each from aedes campestris and psorophora signipennis mosquitoes collected in south central new mexico in august 1985, were shown by neutralization tests to be identical to each other, but not to any of more than 250 arthropod-borne and other viruses. electron microscopy of 1 isolate (85-488nm, chosen as the prototype) indicated that this strain shares morphologic characteristics with viruses of the family rhabdoviridae. indirect fluorescent antibody tests indicated that th ... | 1988 | 3061310 |
| a comparison of cocal and vesicular stomatitis virus, serotypes new jersey and indiana. | 1967 | 4290001 | |
| ultrastructure of cocal, indiana, and new jersey serotypes of vesicular stomatitis virus. | 1967 | 4290652 | |
| complement-fixing antibodies as a measure of immunity of cattle to the virus of vesicular stomatitis new jersey. | 1966 | 4290881 | |
| structural proteins of vesicular stomatitis viruses. | three major and three minor structural proteins were identified by polyacrylamide gel electrophoresis of purified infectious virions of the indiana serotype of vesicular stomatitis (vs) virus disrupted with acetic acid, 0.5 m urea, sodium dodecyl sulfate (sds), and 2-mercaptoethanol. molecular weights of the six virion proteins were estimated by comparative electrophoretic migration of known marker proteins in the presence of sds. the following values were obtained: major proteins p6 congruent w ... | 1969 | 4306194 |
| pathogenesis of vesicular stomatitis virus new jersey infection in the adult hamster and mouse. | 1968 | 4316895 | |
| proteins of vesicular stomatitis virus. ii. immunological comparisons of viral antigens. | the antigens of the nucleoprotein core and the coat of vesicular stomatitis virus (vsv) particles of the indiana serotype were prepared and purified by sucrose gradient fractionation. antibody was prepared separately to each of the two antigen fractions. by immunological procedures, it was shown that soluble antigens of vsv preparations sedimenting at 20s and in the leading edge of the 6s region are antigenically related to vp3, the protein of the virus core, whereas the 6s soluble antigen cross ... | 1970 | 4319479 |
| vesicular stomatitis (vsnj) strain. new jersey (hazelhurst). | 1970 | 4321617 | |
| [study of the co 2 sensitivity symptom induced by vesicular stomatitis virus in drosophila melanogaster. i. vsv of the new jersey serotype and the cocal virus]. | 1971 | 4329708 | |
| comparison of structural polypeptides from vesicular stomatitis virus (indiana and new jersey serotypes) and cocal virus. | 1972 | 4340280 | |
| protein synthesis in bhk21 cells infected with vesicular stomatitis virus. ii. ts mutants of the new jersey serotype. | 1972 | 4343111 | |
| genetic and physiological properties of temperature-sensitive mutants of cocal virus. | temperature-sensitive (ts) mutants of cocal virus (vsv cocal) were isolated after treatment with the base analogue mutagen, 5-fluorouracil. these mutants could be classified into four mutually complementing groups. weak complementation was detected between certain pairs of vsv cocal ts mutants and ts mutants of vesicular stomatitis virus (vsv) indiana, but no complementation was observed with ts mutants of vsv new jersey. two complementing ts mutants of chandipura virus, an unrelated rhabdovirus ... | 1973 | 4359947 |
| rna transcription by the virion polymerases of five rhabdoviruses. | the presence of a virion-associated rna-dependent rna polymerase in five serologically distinct rhabdovirus isolates (vesicular stomatitis virus [vsv] indiana, vsv new jersey, cocal, chandipura, and piry viruses) has been demonstrated. the enzyme for each virus has been shown to be a transcriptase capable of synthesizing in vitro rna which is complementary to the viral genome. by sequence analyses it has been shown that, for each rhabdovirus, transcription is multiply initiated with specific 5' ... | 1974 | 4362867 |
| reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (indiana serotype), chandipura, vesicular stomatitis (new jersey serotype), and cocal viruses. | rna transcriptase activities have been reconstituted from fractionated components of four rhabdoviruses: vesicular stomatitis virus (vsv) (indiana serotype), cocal, vsv (new jersey), and chandipura viruses. heterologous reconstitution of transcription has been observed between template and enzyme fractions of vsv indiana and cocal viruses but not to any significant extent between components of the other two viruses with vsv indiana template or enzyme. homologous reconstitution of infectivity has ... | 1974 | 4365327 |
| isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus, new jersey serotype. | forty-eight temperature-sensitive (ts) mutants have been isolated from a wild-type strain of the new jersey serotype of vesicular stomatitis virus (vsv) after exposure to the base analogue mutagen 5-fluorouracil. of these mutants, 47 have been classified into 6 nonoverlapping complementation groups containing 21, 17, 4, 3, 2, and 1 mutant, respectively (1 mutant remaining unallocated). the ribonucleic acid (rna) phenotype of 23 of these mutants has been established. four of the six groups contai ... | 1971 | 4366429 |
| [titration of interferon using a new type vsv (new jersey serotype) (author's transl)]. | 1974 | 4377339 | |
| specific interactions of vesicular stomatitis virus l and ns proteins with heterologous genome ribonucleoprotein template lead to mrna synthesis in vitro. | two dissociable proteins, l and ns, and n-rna template were purified from two serologically distinct vesicular stomatitis viruses, indiana [vsv(ind)] and new jersey [vsv(nj)]. requirements for rna synthesis in heterologous reconstitution reactions in vitro were studied. the l and ns proteins of vsv(nj) failed to synthesize full-length leader rna and mrnas in vitro when reconstituted with n-rna(ind) template. however, when purified homologous ns(ind) was added to the reaction mixture, mrna synthe ... | 1984 | 6088788 |
| complete nucleotide sequence of the mrna coding for the n protein of vesicular stomatitis virus (new jersey serotype). | the nucleotide sequence of the mrna encoding the nucleocapsid protein of the new jersey serotype (ogden strain) of vesicular stomatitis virus (vsv) was determined from two overlapping cdna clones spanning almost entirely the coding region of the mrna. the 5'-terminal noncoding sequence present in the mrna but not in the cdna clones was determined from a primer extended to the 5' terminus of the mrna. the mrna is 1329 nucleotides long (excluding polyadenylic acid) and encodes a protein of 422 ami ... | 1984 | 6091339 |
| carajas and maraba viruses, two new vesiculoviruses isolated from phlebotomine sand flies in brazil. | two new rhabdoviruses, designated carajas and maraba, are described. both were isolated from phlebotomine sand flies (lutzomyia spp.) collected in the amazon basin of brazil. one recovery of carajas virus was made from male sand flies. by complement-fixation and neutralization tests both agents were shown to be members of the vesicular stomatitis virus (vsv) serogroup (genus vesiculovirus). the pathogenicity of the two viruses in mice and vero cells is similar to that of vsv-indiana and vsv-new ... | 1984 | 6091472 |
| functional relationships within the new jersey serotype of vesicular stomatitis virus: genetic and physiological comparisons of the hazelhurst and concan subtypes. | seventeen temperature-sensitive mutants of the concan subtype of the new jersey serotype of vesicular stomatitis virus have been isolated following mutagenesis and assigned to two complementation groups: cc/a, containing three mutants, and cc/b, containing 14 mutants. prototype mutants of these two concan groups efficiently complemented prototype mutants of the hazelhurst complementation groups (with the exception of the corresponding group) which correspond to genes specifying the l, n, m and n ... | 1984 | 6092522 |
| immunogenicity in mice of temperature-sensitive mutants of vesicular stomatitis virus: early appearance in bronchial secretions of an interferon-like inhibitor. | temperature-sensitive mutants of vesicular stomatitis virus (indiana serotype) rapidly induced resistance in mice to intranasal challenge infection with the highly virulent wild-type homotypic virus, and to a lesser extent with the heterotypic new jersey serotype. intranasal vaccination with mutant tsg44 (iv) resulted in early appearance (at 12 h) of a bronchial inhibitor which protected mouse l cells and chick embryo cells against infection with vesicular stomatitis virus. this bronchial inhibi ... | 1980 | 6154132 |
| the interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. ii. monoclonal antibodies of nonneutralizing and cross-reactive epitopes of indiana and new jersey serotypes. | 1982 | 6180551 | |
| mediators of protection against lethal systemic vesicular stomatitis virus infection in hamsters: defective interfering particles, polyinosinate-polycytidylate, and interferon. | homologous defective interfering (di) particles protected adult syrian hamsters against lethal systemic infection with vesicular stomatitis virus (vsv) serotype indiana. the di particles had to be biologically active, but did not have to be administered at the same inoculation site as the infectious virus. serum and tissue levels of vsv postinoculation were significantly lower in di-protected animals than in unprotected controls, suggesting that true autointerference was occurring. however, some ... | 1982 | 6180986 |
| antigenic determinants of vesicular stomatitis virus: analysis with antigenic variants. | antigenic variants of vesicular stomatitis virus (vsv) serotypes new jersey and indiana (vsv-nj, vsv-ind) were selected by using a panel of monoclonal antibodies (mab) specific for the major surface glycoprotein (g-protein). the reactivity of antigenic variants with the panel of mab confirmed observations made by competitive binding assays that four distinct antigenic sites (a-d)nj on the vsv-nj g-protein and four partially overlapping sites (a, b1, b2, c)ind on the vsv-ind g-protein are involve ... | 1983 | 6183358 |
| cytotoxic t lymphocytes reactive with vesicular stomatitis virus: analysis of specificity with monoclonal antibodies directed to the viral glycoprotein. | a panel of monoclonal antibodies (mab) reactive with the vesicular stomatitis virus (vsv) glycoprotein (g-protein) was used to inhibit lysis of infected target cells by cytotoxic t lymphocytes (ctl) reactive with vsv serotypes indiana and new jersey (vsv-ind, vsv-nj). mab to distinct epitopes on the vsv-nj g-protein were able to block lysis of vsv-nj-infected targets using anti-vsv-nj ctl. furthermore, when a combination of mab to three distinct epitopes was used, increased inhibition was observ ... | 1983 | 6185583 |
| comparative inhibition of cellular transcription by vesicular stomatitis virus serotypes new jersey and indiana: role of each viral leader rna. | we compared the ability of the leader rnas of the new jersey and indiana serotypes of vesicular stomatitis virus to inhibit transcription in infected host cells. the level of cellular rna synthesis in cells infected with either serotype was drastically reduced by 5 h after infection. studies with uv-inactivated virus demonstrated that shutoff of cellular rna synthesis directly correlated with the ability of the infecting virus to transcribe its plus-stranded leader rna. although both serotypes i ... | 1983 | 6193289 |
| interactions of plus and minus strand leader rnas of the new jersey serotype of vesicular stomatitis virus with the cellular la protein. | the new jersey serotype of vesicular stomatitis virus (vsv-nj) was found to synthesize a minus strand leader rna of 44-46 bases long and a plus strand leader rna of 47-50 bases long in infected cells. the minus strand leader rna of vsv-nj was found associated with the host cell la protein in infected cells by immunoprecipitation with antisera from patients with systemic lupus erythematosus. these results differ from those reported previously (j. wilusz , m. g. kurilla , and j. d. keene (1983). p ... | 1984 | 6203219 |
| comparisons of nucleotide sequences in the genomes of the new jersey and indiana serotypes of vesicular stomatitis virus. | nucleotide sequences of around 200 residues were determined adjacent to the 3' terminus of the genome rna of vesicular stomatitis virus, new jersey serotype, and adjacent to the 3'-terminal polyadenylic acid tract of the n protein mrna of the same virus. these sequences were compared with the corresponding sequences previously determined for the indiana serotype of vesicular stomatitis virus. the sequences obtained for the two strains were readily aligned, showing 70.8% homology overall. examina ... | 1980 | 6245255 |
| biochemical characterization of the tse1 mutant of vesicular stomatitis virus (new jersey). alterations in the ns protein. | alterations in the ns protein of the tse1 mutant of vesicular stomatitis virus (new jersey serotype) appear to be responsible for its temperature-sensitive phenotype. the ns proteins of thermostable revertants of tse1 migrated in polyacrylamide gels containing sodium dodecyl sulfate with apparent sizes which were identical to tse1 ns, or which were 5% or 14% larger than tse1 ns. these novel differences persisted during electrophoresis in 10% and 12.5% acrylamide gels, and in gels with gradients ... | 1980 | 6251081 |
| intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus rna. | the base sequence at the 3' end of vesicular stomatitis virus rna was determined by using terminal labels and chemical rna sequencing. the leader rna was complementary to 47 bases at the 3' terminus, whereas the nucleocapsid gene (n) began 51 nucleotides from the 3' end of the genomic rna. the intervening bases were 3'...gaaa...5' for the indiana serotype and 3'...gaaaa...5' for the new jersey serotype. the complements of these bases did not appear in either the leader rna or the n mrna. this se ... | 1980 | 6251249 |
| poly(a)-adjacent sequence of the 14.5 s mrna of vesicular stomatitis virus (new jersey serotype). | 1980 | 6251611 | |
| enhanced mutability associated with a temperature-sensitive mutant of vesicular stomatitis virus. | temperature-sensitive (ts) mutant tsd1 of vesicular stomatitis virus, new jersey serotype, is the sole representative of complementation group d. clones derived from this mutant exhibited three different phenotypes with respect to electrophoretic mobility of the g and n polypeptides of the virion in sodium dodecyl sulfate-polyacrylamide gel. analysis of non-ts pseudorevertants showed that none of the three phenotypes was associated with the temperature sensitivity of mutant tsd1. additional phen ... | 1981 | 6268829 |
| measurement of surface antigen by specific bacterial adherence and scanning electron microscopy (saba/sem) in cells infected by vesiculovirus ts mutants. | temperature-sensitive (ts) mutants of the rhabdoviruses vesicular stomatitis virus and chandipura virus have been used to measure the appearance of virus antigen on the surface of infected cells by the technique of surface analysis by bacterial adherence and scanning electron microscopy (saba/sem). the number of staphylococci specifically adhering to antiserum-treated infected ptk-2 or bsc-1 cells at permissive (31 degrees c) and restrictive (39 degrees c) temperatures was followed in time-cours ... | 1982 | 6279772 |
| in vitro and in vivo inhibition of primary transcription of vesicular stomatitis virus by a defective interfering particle. | a unique defective interfering (di) particle, generated by a heat-resistant (hr) mutant of indiana serotype vesicular stomatitis virus, was capable of inhibiting primary transcription by heterologous new jersey serotype virions. the correlation between this phenomenon and the lowering of viral yields from doubly infected cells was investigated by the construction of chimeric di particles containing the hr di particle genome with a thermolabile polymerase. at the nonpermissive temperature, these ... | 1982 | 6283111 |
| linear mapping of tryptophan residues in vesiculovirus m and n proteins by partial chemical cleavage. | nonlimit chemical cleavage at tryptophan residues of protein labeled at the amino terminus afforded a simple procedure for generating specific fragments and for mapping tryptophan positions. a comparison of the matrix (m) and nucleocapsid (n) proteins of four members of the vesiculovirus group by this procedure suggests considerable conservation of tryptophan number and location in the four serotypes examined. | 1982 | 6283149 |
| use of a hybrid infectivity assay to analyze primary transcription of temperature-sensitive mutants of the new jersey serotype of vesicular stomatitis virus. | a hybrid infectivity assay specific for primary transcription was developed to analyze the production of functional mrnas by vesicular stomatitis virus. a template prepared from wild-type virions of the new jersey serotype of vesicular stomatitis virus was reconstituted with rna polymerase proteins from the wild type or temperature-sensitive mutants, and the in vivo temperature sensitivity of the polymerase was determined by infectivity assay. the data demonstrate that the new jersey temperature ... | 1982 | 6287014 |
| characterization of the electrophoretic mobility mutation in the n protein of the tsd1 mutant of vesicular stomatitis virus new jersey serotype. | some isolates of the temperature sensitive mutant tsd1 of complementation group d of vesicular stomatitis virus of new jersey serotype have a nucleocapsid (n) protein which shows an increased electrophoretic mobility on sodium dodecyl sulfate--polyacrylamide gel electrophoresis (sds-page) when compared with wild type. utilizing techniques involving specific chemical cleavage at tryptophan or methionine residues, as well as enzymatic cleavage with carboxypeptidases a and b, we have determined tha ... | 1982 | 6293685 |
| nucleotide sequence of a cdna clone encoding the entire glycoprotein from the new jersey serotype of vesicular stomatitis virus. | the nucleotide sequence of the mrna encoding the glycoprotein from the new jersey serotype of vesicular stomatitis virus (vsv) was determined from a cdna clone containing the entire coding region. the sequence of 12 5'-terminal noncoding nucleotides present in the mrna but not in the cdna clone was determined from a primer extended to the 5' terminus of the mrna. the mrna is 1,573 nucleotides long (excluding polyadenylic acid) and encodes a protein of 517 amino acids. only six nucleotides occur ... | 1983 | 6298453 |
| identification of hitherto unrecognized arboviruses from ecuador: members of serogroups b, c, bunyamwera, patois, and minatitlan. | three hundred seventy-nine virus isolates were obtained from mosquitoes collected and sentinel hamsters exposed in coastal ecuador from 1974 to 1978. these included four alphaviruses [venezuelan equine encephalitis 1b (1), venezuelan equine encephalitis 1d (35), western equine encephalitis (1) and eastern equine encephalitis (4)]; two flaviviruses [st. louis encephalitis (3) and naranjal (6)]; 11 bunyaviruses [maguari (243), playas (3), vinces (33), turlock (2), abras (5), babahoyo (3), acara (2 ... | 1983 | 6309029 |
| viral membrane glycoproteins: comparison of the amino terminal amino acid sequences of the precursor and mature glycoproteins of three serotypes of vesicular stomatitis virus. | the nh2-terminal amino acid sequences of the envelope glycoproteins and the in vitro synthesized, nonglycosylated precursors of the glycoproteins of three serotypes, namely indiana (toronto), cocal, and new jersey (concan) of vesicular stomatitis virus were determined. a comparison of the sequences showed little homology in the signal peptides present in the nonglycosylated precursors except for their high hydrophobic amino acid content. in contrast, the nh2-terminal amino acid sequences of the ... | 1983 | 6310873 |
| unusual heterogeneity in the glycosylation of the g protein of the hazelhurst strain of vesicular stomatitis virus. | the asparagine-linked oligosaccharides of the g protein of the hazelhurst subtype of the new jersey serotype of vesicular stomatitis virus (vsv) have been compared with the oligosaccharides from the g protein of the well-characterized indiana serotype of vsv, with baby hamster kidney cells in monolayer culture as the host for both viruses. [3h]glucosamine- and [3h]mannose-labeled glycopeptides from the g protein of purified virus were analyzed by the combined techniques of endo-beta-n-acetylgluc ... | 1983 | 6314902 |
| superinfection exclusion by vesicular stomatitis virus. | the infection of baby hamster kidney (bhk21) cells by the indiana strain of vesicular stomatitis virus (vsv) causes a rapid loss of the ability of the cells to be superinfected by vsv virions or defective-interfering particles. this exclusion phenomenon is at the level of virus penetration and requires viral gene expression and a functional vsv transmembrane glycoprotein g. infection with the new jersey serotype of vsv also inhibits the uptake of the indiana serotype. however, infection of bhk21 ... | 1983 | 6316647 |
| nucleotide sequence and secondary structure of vsv leader rna and homologous dna involved in inhibition of dna-dependent transcription. | we have analyzed the nucleotide sequences and secondary structure required for the transcriptional inhibitory activity of the plus-strand leader rna of vesicular stomatitis virus (vsv) in a reconstituted hela cell transcription system using the adenovirus-2 late promoter (lp) and virus-associated (va) genes as templates. the new jersey serotype (vsvnj) leader and the leader of the indiana serotype (vsvind) both contain cleavage sites for the double-strand-specific ribonuclease v1, and these site ... | 1984 | 6319029 |
| structural and functional characterization of the rna-positive complementation groups, c and d, of the new jersey serotype of vesicular stomatitis virus: assignment of the m gene to the c complementation group. | the structural and functional lesions in the rna-positive complementation groups, c and d, of the new jersey serotype (hazelhurst subtype) of vesicular stomatitis virus have been characterized. the m protein of the temperature-sensitive mutant c1, the prototype of the c complementation group, was degraded at the restrictive temperature in vivo, and was resolved from the wild-type m protein by sds-polyacrylamide gel electrophoresis and nonequilibrium ph gradient electrophoresis. coreversion of th ... | 1984 | 6320536 |
| role of fatty acid acylation of membrane glycoproteins. absence of palmitic acid in glycoproteins of two serotypes of vesicular stomatitis virus. | the presence of fatty acids bound to the glycoprotein in a number of serotypes of vesicular stomatitis virus was investigated by growing the virus in the presence of [3h]palmitic acid. [3h]palmitate was efficiently incorporated into g proteins of the serotypes indiana, piry, and chandipura but was not detected in g proteins from cocal and three different strains of new jersey serotypes. cerulenin, an inhibitor of fatty acid acylation, also did not inhibit the maturation of cocal serotype. these ... | 1984 | 6325406 |
| protection against lethal viral infection by neutralizing and nonneutralizing monoclonal antibodies: distinct mechanisms of action in vivo. | monoclonal antibodies (mab) reactive with the glycoprotein of vesicular stomatitis virus (vsv) serotypes indiana (vsv-ind) and new jersey (vsv-nj) were used to protect mice against lethal infection. mab which reacted with a number of distinct epitopes and which could neutralize the virus in vitro could also protect against infection in vivo. mab which could not neutralize the virus in vitro but which were specific for the glycoprotein of a single serotype were also able to protect mice against l ... | 1984 | 6328040 |
| growth and transovarial transmission of chandipura virus (rhabdoviridae: vesiculovirus) in phlebotomus papatasi. | chandipura virus multipled in sand flies (phlebotomus papatasi) following intrathoracic inoculation. within 24 hours, mean virus titers in infected flies increased approximately 4 logs. experimentally infected p. papatasi transmitted the virus by bite to newborn mice and by transovarial transmission to their progeny. eight percent of the f1 offspring of experimentally infected female parents were infected with chandipura virus. | 1983 | 6683084 |
| susceptibility of various animals to the vesiculovirus piry. | to determine the pathogenic potential of the vesiculovirus piry for domestic animals, two ponies, two steers, three sheep, three goats and three pigs were inoculated intradermally in the tongue or, in the case of the pigs, in the snout, heel and coronary band. inoculated animals were housed in one room and allowed to mingle freely with an equal number of uninoculated contact animals of each species. clinical signs of infection, consisting of elevated temperature and ulcers at the inoculation sit ... | 1984 | 6747282 |
| [the perinet virus, rhabdoviridae, of the vesiculovirus type isolated in madagascar from culicidae]. | 1982 | 7186788 | |
| incompetence of white-tailed deer as amplifying hosts of vesicular stomatitis virus for lutzomyia shannoni (diptera: psychodidae). | sand flies, lutzomyia shannoni dyar, were allowed to feed on 3 white-tailed deer, odocoileus virginiana zimmermann, that previously had been infected with the new jersey serotype of vesicular stomatitis (vsnj) virus. flies fed in the lower abdominal area of each deer on days 1-5 postinfection. a blood sample, nasal swab, and throat swab were taken during each feeding trial and examined for virus. blood-fed flies were held for 4-5 d following the bloodmeal and tested for vsnj virus infection. vsn ... | 1995 | 7473631 |
| incompetence of domestic pigs as amplifying hosts of vesicular stomatitis virus for lutzomyia shannoni (diptera: psychodidae). | seven domestic pigs, sus scrofa l., were infected by intradermal inoculation at 3 different sites with the new jersey serotype of vesicular stomatitis (vsnj) virus. laboratory-reared lutzomyia shannoni dyar sand flies, a suspected biological vector of vsnj virus, were allowed to feed on pigs at the lower abdomen or at sites of their own selection on days 1-7 and on day 10 postinfection. blood samples were taken from infected swine concomitant with most feeding trials and tested for the presence ... | 1995 | 7473632 |
| cow, farm, and ecologic risk factors of clinical vesicular stomatitis on costa rican dairy farms. | twenty-two costa rican dairy herds were actively monitored for vesicular diseases between november 1991 and march 1992, in anticipation of the annual dry season occurrence of vesicular stomatitis (vs). thirty-nine confirmed cases of vs were concentrated on nine farms, resulting in an average herd incidence rate of 11.1% of mature cattle for the affected farms, or 2.6% for all farms. affected cattle were generally older (average age = 5.4 years), with seven-year-old cows having the highest age-sp ... | 1995 | 7485685 |
| common distribution of antigenic determinants and complementation activity on matrix proteins of two vesicular stomatitis virus serotypes. | to compare the antigenic and functional domains of the matrix (m) proteins of vesicular stomatitis virus (vsv) serotypes indiana (vsv-ind) and new jersey (vsv-nj), deletion mutants and chimeras were cloned in pbsm13 and expressed as in-frame lacz fusion proteins in escherichia coli. non-cross-reactive monoclonal antibodies directed to the two antigenically distinct m proteins were tested by western blot analysis to map three epitopes of vsv-ind m protein and four epitopes of vsv-nj m protein. ep ... | 1994 | 7512123 |
| enhancement of fish mortality by rhabdovirus infection after immunization with a viral nucleoprotein peptide. | a similar sequence to a mouse immunodominant ctl peptide (syvlqgn, single-letter amino acid code, conserved amino acids underlined) identified in the nucleoproteins of several strains of vesicular stomatitis virus (vsv) (37) was found in the nucleoproteins of viral hemorrhagic septicemia virus (vhsv) of salmonid fish (gyvyqgl in vhsv 07.71 and gyvyqgs in vhsv makah) and not in the nucleoproteins of other rhabdoviruses. the in vivo immunization of fingerling salmonid fish (rainbow trout onchorync ... | 1993 | 7513169 |
| localization of t helper cell epitopes in the vesicular stomatitis virus: the nucleoprotein is responsible for serotype cross-reactive t help. | the glycoprotein (g) of vesicular stomatitis virus (vsv) is known to contain the biologically relevant sites for virus-neutralizing antibodies as well as t helper (th) cell epitopes. the capacity of other vsv proteins to elicit potent th cell responses has not yet been investigated. additionally, a short-lived cross-reactivity between the two serologically distinct vsv serotypes indiana (ind) and new jersey (nj) on the t helper cell level has been reported. here we address the question of whethe ... | 1994 | 7541205 |
| the glycoprotein g of rhabdoviruses. | rhabdoviruses show an rna-containing helically-wound nucleocapsid either enclosed by or enclosing a membrane m protein, surrounded by a lipid bilayer through which dynamic protein trimers made up of non-covalently associated monomers of glycoprotein g (g) project outside. mature monomeric rhabdoviral g has more than 500 amino acids, 2-6 potential glycosylation sites, 12-16 highly conserved cysteine residues, 2-3 stretches of a-d hydrophobic heptad-repeats, a removed amino terminal hydrophobic si ... | 1995 | 7605197 |
| efficient interaction of the vesicular stomatitis virus p protein with the l protein or the n protein in cells expressing the recombinant proteins. | specific in vivo interaction between the phosphoprotein (p) and the large polymerase protein (l) from the indiana serotype of vesicular stomatitis virus was studied using a two-hybrid system. transfection of cho cells with plasmids encoding galpind and vplind fusion proteins resulted in an easily detectable level of cat activity, indicating that pind and lind associate in vivo in the absence of other viral proteins. mutational studies of pind demonstrated that both domains i and ii of pind are i ... | 1995 | 7747458 |
| taxonomy and evolutionary studies on lyssaviruses with special reference to africa. | currently, the lyssavirus genus is composed of four serotypes and the ungrouped european bat lyssaviruses (ebl). using previously described pcr amplification and direct sequencing methods, the n genes of 70 representative lyssaviruses collected from 40 different countries were examined. from the results, a phylogenetic tree with six branches (genotypes) was constructed: genotypes 1-4 correlated with the classification of serotypes 1-4 and in addition ebl 1 and ebl 2 were identified as two furthe ... | 1993 | 7777311 |
| cloning of the chandipura virus phosphoprotein encoding gene and its expression in escherichia coli. | chandipura (chp) virus, a member of the vesiculovirus genus within the rhabdoviridae family, was first isolated from human patients in india. the full length phosphoprotein p gene of chp have been cloned and expressed in escherichia coli using the t7 polymerase-based pet-3 series of expression vectors. under optimal conditions of induction with iptg, the recombinant p protein constituted 35% of the total bacterial protein. the bacterially expressed protein was found to be phosphate-free. deletio ... | 1994 | 7787887 |
| population dynamics of lutzomyia shannoni (diptera: psychodidae) in relation to the epizootiology of vesicular stomatitis virus on ossabaw island, georgia. | population dynamics of lutzomyia shannoni dyar were studied on ossabaw island, ga, to define further the role of this species in the epizootiology of the new jersey serotype of vesicular stomatitis (vsnj) virus. bimonthly collections of sand flies egressing from hollow trees from april to november 1991 indicated that there were three generations of sand flies. data from light trap collections from 1986 through 1989 indicated that similar seasonal cycles occurred during previous years. at this si ... | 1994 | 7815397 |
| hosts of lutzomyia shannoni (diptera: psychodidae) in relation to vesicular stomatitis virus on ossabaw island, georgia, u.s.a. | hosts of lutzomyia shannoni dyar, a suspected biological vector of the new jersey serotype of vesicular stomatitis (vsnj) virus, were determined using an indirect enzyme-linked immunosorbent assay (elisa) of 333 blood-fed female sandflies collected from their diurnal resting shelters on ossabaw island, georgia, u.s.a. sandflies had fed primarily on white-tailed deer (odocoileus virginianus) (81%) and to a lesser extent on feral swine (sus scrofa) (16%), two species of host infected annually with ... | 1994 | 7841487 |
| analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus. | the complete nucleotide sequence of the nucleocapsid (n) gene of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of plant rhabdoviruses, has been determined from cdna clones of both the viral genomic and messenger rnas. the previously identified n gene was adjacent to the 3' leader rna and began at position 91 from the 3' end of the lnyv genome. analysis of the sequence showed that the n mrna contained a 78-nt 5' untranslated region, followed by a 1377-nt open ... | 1994 | 8030210 |
| structural and antigenic analysis of the nucleoprotein of bovine ephemeral fever rhabdovirus. | the nucleotide sequence of the bovine ephemeral fever virus (befv) genome has been determined from the 3' terminus to the end of the nucleoprotein (n) gene. the 3' leader sequence comprises 50 nucleotides and shares a common terminal three nucleotides (3'-ugc-) and a downstream u-rich domain with vesicular stomatitis virus (vsv) and rabies virus. the n gene comprises 1328 nucleotides from the transcription initiation consensus sequence (aacagg) to the conserved transcription termination-poly(a) ... | 1994 | 8046391 |
| determinants of serotype specificity in transcription of vesicular stomatitis virus synthetic nucleocapsids. | transcription of the nucleocapsid template of vesicular stomatitis virus (vsv) is serotype specific, since the viral rna polymerase of the vsv indiana (ind) serotype transcribes the ind nucleocapsid but not the nucleocapsid of the vsv new jersey (nj) serotype and, similarly, the nj rna polymerase transcribes only the nj nucleocapsid. we have prepared synthetic nucleocapsid templates from various combinations of purified leader gene rna and n protein of these two vsv serotypes for analysis of ser ... | 1994 | 8116233 |
| piry virus antibodies in inhabitants of rio de janeiro. | 1993 | 8139469 | |
| genomic organization of lettuce necrotic yellows rhabdovirus. | we have mapped the genome of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of the family rhabdoviridae. we have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded rna genome of lnyv. the lnyv genome appears to contain six genes, the five expected genes coding for the virion proteins, and a sixth gene of unknown function, as for sonchus yellow net virus (synv), a member of the genus nucleorhabdo ... | 1994 | 8178430 |
| first isolations of arboviruses from phlebotomine sand flies in west africa. | for the first time in west africa, arboviruses were isolated from phlebotomine sand fly pools. one strain of chandipura virus (a vesiculovirus), four strains of saboya virus (a flavivirus), and one strain of a not yet identified virus were isolated. three hundred twenty-two pools were established from a population of 33,917 sand flies caught in co2 light traps in the ferlo sahelian region of senegal from november 1991 to december 1992. this is the first isolation of chandipura virus from any art ... | 1994 | 8203705 |
| [comparison of two neutralization tests, used in vesiculovirus piry serodiagnosis]. | a serological survey of piry was undertaken of the sera of inhabitants of catolância-bahia state, brazil. serum results obtained by a vesiculovirus neutralization test of c6/36 cells read by elisa are compared with those obtained by the classic technique, carried out on newborn mice. the agreement between the results was as high as 98.7% of the 204 sera tested and the neutralization test of c6/36 cells was chosen as the most suitable technique for the sero-survey testing. | 1993 | 8209171 |
| hiv-1 pseudotype virus containing a cocal virus genome and an hiv envelope: construction, assay and use. | a method is described for the production and assay of pseudotype viruses between human immunodeficiency virus type 1 (hiv-1) and cocal virus (cov), containing an hiv-1 envelope and a cov genome (cov(hiv)). cov(hiv) pseudotype virus is a useful tool for the investigation of a variety of questions regarding hiv entry into susceptible cells, including steps in virus binding, fusion, and internalization, and the role of molecules which inhibit entry. cov, a rhabdovirus closely related to vesicular s ... | 1993 | 8263122 |
| expression of the vesicular stomatitis virus nucleocapsid protein gene in escherichia coli: analysis of its biological activity in vitro. | the nucleocapsid (n) protein genes of vesicular stomatitis virus, serotypes new jersey and indiana, were expressed in escherichia coli bl21(de3) transformed by the recombinant expression vector pet-3a containing n genes under the control of the t7 rna polymerase promoter. following induction with isopropylthiogalactopyranoside, the n protein was synthesized, representing 10 to 20% of total cellular protein. the n protein remained in a nonaggregated form in 1 m nacl solution. the purified n prote ... | 1993 | 8382400 |
| application of indirect elisa for detection of bovine antibodies against vesicular stomatitis viruses. | two indirect enzyme-linked immunosorbent assays (i-elisas) are described for the detection of bovine serum antibody to the new jersey (nj) and indiana (in) vesicular stomatitis viruses (vsv). serum samples at a dilution of 1:200 were incubated with binary ethylenimine-inactivated vsv-nj and vsv-in type-specific antigens preadsorbed to microtiter plates. bound antibodies were detected by a murine monoclonal antibody to bovine igg1 conjugated with horseradish peroxidase. the performance of each i- ... | 1993 | 8385501 |
| molecular diversity of the lyssavirus genus. | the sequence of 5568 nucleotides of the 3' moiety of the mokola virus genome (serotype 3 of lyssaviruses) encompassing the nucleoprotein (n), phosphoprotein, matrix protein, and glycoprotein genes is presented and compared to that of the vaccinal strains of serotype 1. it allowed us to determine consensus sequences derived from the transcriptional start/stop signals and the order of protein conservation (nucleoprotein > matrix protein > phosphoprotein) in lyssaviruses. the sequences of the n gen ... | 1993 | 8386891 |
| effect of forest type on the distribution of lutzomyia shannoni (diptera: psychodidae) and vesicular stomatitis virus on ossabaw island, georgia. | we studied the effects of three forest types on multiple factors that are believed to influence the transmission of the new jersey serotype of vesicular stomatitis (vsnj) virus on ossabaw island, ga. these factors included availability of tree hole diurnal resting habitat for the presumed sand fly vector, lutzomyia shannoni dyar; relative abundance of l. shannoni; prevalence of vsnj virus infection in sand flies; and prevalence of vsnj virus antibodies in wild swine. tree hole availability, sand ... | 1993 | 8389877 |
| formalin inactivation of vesicular stomatitis virus impairs t-cell- but not t-help-independent b-cell responses. | the effects of formalin on the infectivity and immunogenicity of vesicular stomatitis virus (vsv) serotype indiana were investigated. we found that formalin inactivation of vsv prevents infection of vero cells in a concentration- and time-dependent manner, as shown by fluorometric cell analysis and inhibition of plaque formation. inactivated vsv failed to induce significant cytotoxic t-lymphocyte responses in vivo or after restimulation in vitro. in contrast, the early immunoglobulin m (igm) res ... | 1993 | 8389912 |
| immunological characterization of the vsv nucleocapsid (n) protein expressed by recombinant baculovirus in spodoptera exigua larva: use in differential diagnosis between vaccinated and infected animals. | vesicular stomatitis is a viral disease of cattle, pigs, and horses. the disease is characterized by vesicular lesions on the epithelium of the mouth, feet, and teats. the pathological lesions are virtually indistinguishable from that of foot-and-mouth disease. we have developed a recombinant baculovirus that expresses the nucleocapsid (n) protein of the new jersey serotype of vesicular stomatitis virus (vsvnj) in insect cells (sf9) and larvae (spodoptera exigua). the gene was expressed under co ... | 1993 | 8390753 |