Publications

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reaction of lung tissue of goats to rinderpest caprinized virus. 19685651748
[application of the kinetic sero-neutralization method to the study of neutralizing antibodies to rinderpest virus in cattle, sheep and goats]. 19675626099
experimental infection of sheep and goats with attenuated and virulent strains of rinderpest virus. 19675625712
[presence of anti-rinderpest antibodies in dromedaries in chad]. 19675625567
development of a cell culture system susceptible to measle, canine distemper, and rinderpest viruses. 19675600774
susceptibility of cattle, buffaloes and swine in cambodia to lapinized rinderpest virus. 19715569699
an indirect hemagglutination test of rinderpest virus. 19715569698
studies on lapinised rinderpest virus. 19715566706
the pathogenesis of rinderpest in the lymph nodes of cattle. light and electron microscopic studies. 19715540659
[immunological studies on bovine pleuropneumonia. xi. a combined living antibovipestic antiperipneumonic vaccine inoculated at the same time. concept, production, controls]. 19705535125
studies on rinderpest culture vaccine. 3. stability of the lyophilised product. 19705533977
a plaque assay system for rinderpest virus and its use in characterising virus adsorption. 19705533699
effect of rinderpest vaccination of spermatogenesis in bulls. 19705462213
a new method of evaluation of the potency of anti rinderpest serum. 19705439960
the immunologic concept of heterotypic resistance. 19705422524
production of veterinary vaccines in mammalian cell cultures. 19705416447
[adaptation in microtest of the seroneutralization technic by the kinetic method for the research and titration of antibodies neutralizing the rinderpest virus]. 19695408391
[clinical and immunologic behavior during rinderpest contamination of cattle vaccinated several years ago against rinderpest with cell culture vaccines]. 19695408390
studies on rinderpest culture vaccine. ii. factors influencing the accuracy of vaccine potency tests. 19695392352
[recently acquired knowledge on cattle plague and its virus]. 19665333398
investigations on the incidence of rinderpest virus infection in game animals of n. tanganyika and s. kenya 1960-63. 19675233989
studies on the pathogenesis of rinderpest in experimental cattle. iv. proliferation of the virus following contact infection. 19655215637
[comparative morphology of measles, distemper and rinderpest viruses]. 19715169406
susceptibility of cattle, buffaloes and swine in cambodia to lapinized-avianized rinderpest virus. 19715167327
[rinderpest protection of bovines by measles virus. ii. vaccination of calves born from cows vaccinated with the mb 113 strain]. 19715137767
studies on rinderpest culture vaccine. iv. the stability of the reconstituted product. 19715103430
congenital infection of cattle with the herpesvirus causing malignant catarrhal fever. 19725017823
a comparative study of peste des petits ruminants and kata in west african dwarf goats. 19715006087
subacute sclerosing panencephalitis, measles, rabies and progressive multifocal leukoencephalopathy. 19715000224
a comparative electron microscopic study on the morphogenesis of canine distemper and rinderpest viruses. 19714994230
[biological aspects of the multiplication of the plague virus of small ruminants or srp virus on cell cultures]. 19684985884
use of vero cells for titration of rinderpest virus and its neutralizing antibody.primary bovine embryonic kidney cell was successfully replaced by the green monkey cell line (vero) for the isolation and seroneutralization of rinderpest virus.19704985833
[some fundamental research on rinderpest virus. 2. rinderpest haemagglutinin. antigenic relationships of the rinderpest and measles viruses]. 19684984507
serologic response in monkeys inoculated with rinderpest and measles viruses. 19694980999
clinical and immune response of alien hosts to inoculation with measles, rinderpest, and canine distemper viruses. 19654956660
[cattle-plague and immunofluorescence. application to the differential diagnosis of cattle-plague infection]. 19704934929
relationships among measles, canine distemper and rinderpest viruses. 19684886887
[studies on the rinderpest virus under application of cell cultures]. 19664869805
[small ruminant plague and its prevention in senegal and in western africa]. 19734807991
[inopportune cattle mucosal diseases associated with rinderpest vaccine]. 19724677385
[modern methods and technics in the experimental diagnosis of bovine plague]. 19734617907
pathogenesis of rinderpest virus infection in rabbits. ii. effect of rinderpest virus on the immune functions of rabbits.rinderpest virus infection was shown to induce marked suppression of both humoral antibody response and cell-mediated immunity in rabbits. the virus exhibited a suppressive effect on primary antibody response as indicated by a decrease in numbers of plaque-forming cells (immunoglobulin [ig]m) and hemagglutinating antibody titers of both igm and igg types to sheep red blood cells, whereas there was no detectable effect of the virus on the production of memory cells. virus-induced suppression of c ...19744593340
pathogenesis of rinderpest virus infection in rabbits. i. clinical signs, immune response, histological changes, and virus growth patterns.rabbits were intravenously inoculated with an attenuated rinderpest virus (l strain), and general patterns of the disease were investigated. the rabbits developed fever with concomitant occurrence of diarrhea and lymphopenia. early production of interferon was followed by a rise of neutralizing antibody. histological examinations revealed an involvement of all of the lymphoid tissues, with primary lesions consisting of necrosis of the lymphoid follicles and formation of giant cells. immunofluore ...19744593339
observations on the behaviour of rinderpest virus in immune animals challenged intransally. 19744471456
serum electrophoretic pattern of buffalo calves vaccinated with cell culture rinderpest vaccine. 19744464256
active immunization of cattle with killed vaccines prepared from cell-cultured rinderpest virus. 19744425313
inactivation of rinderpest and ecbo viruses by hydroxylamine. 19674383037
ribonucleic acid synthesis in rinderpest virus infected cells. 19744374647
observations on pathogenic organisms in the airborne state. 19724367142
a diseases of sokoto goats resembling "peste des petits ruminants". 19724366855
factors influencing the epidemiology and epizootiology of airborne diseases. 19714342868
cytopathic effect in cell cultures of rinderpest virus and some other viruses causing rinderpest-like diseases. 19704333486
clinical and serologic response of american white-collared peccaries to african swine fever, foot-and-mouth disease, vesicular stomatitis, vesicular exanthema of swine, hog cholera, and rinderpest viruses. 19694322827
the immunodepressive effect of rinderpest virus. 19704320166
presence of 2 different viral agents in brain cells of patients with subacute sclerosing panencephalitis. 19704316388
observations on cultured cells infected with rinderpest virus by means of fluorescent antibody technique. 19694309157
comparative cytopathology of rinderpest and bovine parainfluenza 3 viruses in cell cultures. 19664290608
colostral transfer of rinderpest neutralizing antibody to offspring of vaccinated dams.the transfer of maternal antibodies to friesian and buffalo calves born of dams vaccinated against rinderpest was through colostrum only. colostral antibody titers at the time of parturition were higher than the serum titer. two hours after suckling, a high level of rinderpest neutralizing antibodies was detected in the sera of newborn animals. the half-life of maternal antibodies in buffalo and friesian calves was found to be approximately 33 and 29 days respectively. by the age of 7-8 months, ...19674229186
physico-chemical characterisation of rinderpest virus. 19744216758
[study of the immunostimulating activity of 3 imidazole derivatives with anthelminthic action, on bovipestic, peripneumonic and anthrax immunogenesis]. 19744216052
natural infection of cynomolgus monkeys with measles virus. 19734214280
ferritin-tagged antibody cross-reactions among rinderpest, canine distemper, and measles viruses. 19734201833
comparative immunofluorescent studies on measles, canine distemper, and rinderpest viruses. immunofluorescence of measles, distemper, and rinderpest viruses. 19704195094
chemical structure of african swine fever virus investigated by electron microscopy. 19674168517
[studies of cattle plague virus with the use of cell cultures]. 19664168065
further studies on the immunologic relationships among measles, distemper, and rinderpest viruses. 19744139195
freeze-dried vaccine against rinderpest: stability and activity study.a freeze-dried vaccine against rinderpest was prepared from modified virus multiplied in calf kidney cell culture. characteristics of the vaccine are as follows: high titre after freeze-drying (10(4) ccid50/dose), well-adapted freeze-drying stabilizer which ensures maintenance of the infective titre of the vaccinal virus, even under severe conditions (3.5 days at +45 degrees c), use of an appropriate solvent: magnesium sulphate molar solution or more simply physiological saline (for stability af ...19854092427
the ultrastructure of peste des petits ruminants virus. 19854050210
comparison of messenger rnas induced in cells infected with each member of the morbillivirus group.virus-specific mrnas radiolabelled with [32p]orthophosphate in the presence of actinomycin d were extracted from the cytoplasm of vero cells infected with each of the known morbilliviruses: measles virus, canine distemper virus, rinderpest virus, and peste des petits ruminants virus. when analysed on denaturing agarose-formaldehyde gels the major rna species from all viruses in the group were identical, except for canine distemper virus where one of the virus-specific mrnas (mrna 5), which proba ...19854013082
simple techniques for increasing the batch size of rinderpest cell culture vaccine. 19853992673
antibody response of cattle to rinderpest vaccine.antibody production was studied in cattle infected with rinderpest vaccine virus. vaccinated cattle produced both igm and igg serum antibodies. the igg antibodies were mainly those of igg2 subclass. no iga antibody response was detected in vaccinated animals.19853984206
adaptation of the lapinized rinderpest virus to in vitro growth and attenuation of its virulence in rabbits.a lapinized rinderpest virus, the l strain, which is virulent in rabbits and had been grown only in rabbits, was adapted to grow in vero cells by the fusion of vero cells with virus-infected rabbit spleen cells in the presence of polyethylene glycol, and subsequently passaged in vero cells by co-culture technique. after several passages, free virus was produced at high titre. the vero cell-adapted virus acquired the ability to infect several cell lines which were non-permissive to the unadapted ...19863944587
notes on immunoprecipitin reactions with rinderpest virus. 19853922099
the differential growth of virulent and avirulent strains of rinderpest virus in bovine lymphocytes and macrophages.rinderpest virus (rv) grew readily in cultures of purified bovine peripheral blood lymphocytes and udder macrophages. the growth of three strains of rv was compared and there appeared to be a relationship between increasing virulence and increased ability to infect lymphocytes and macrophages. the proportion of infected cells as determined by the presence of virus antigens was a better indicator of affinity between a strain and cell type than production of new infectious virus. rv grew better in ...19853873515
continuing presence of rinderpest virus as a threat in east africa, 1983-1985.the re-emergence of rinderpest virus in east africa in 1979 caused widespread outbreaks of disease and subclinical infection throughout the region until mid-1983. subsequent massive emergency vaccination campaigns have been successful in eliminating clinical rinderpest from tanzania and preventing its spread southwards. unfortunately the virus is still endemic in north-eastern uganda and has recently caused epidemic outbreaks with high mortality in cattle in that country. in kenya, buffaloes (sy ...19873824842
development and stability of rinderpest virus antigens in cattle tears and lymph nodes.diffusible rinderpest virus antigens were demonstrated in increasing quantities in ocular and lymph node biopsies from rinderpest-infected cattle using agar gel immunodiffusion (agid) and counterimmunoelectrophoresis (ciep) tests. positive samples were detected from the second day of pyrexia to two days after death. the antigens in ocular secretions and lymph nodes were thermolabile being destroyed within five minutes at 56 degrees c and within two weeks at 4 degrees c.19863705176
application of solid phase aggregation of coated erythrocytes technique for detection of rinderpest antigen.a solid phase aggregation of coated erythrocytes (space) technique was standardized and used for the detection of rinderpest antigen in 129 samples of 9 visceral organs; from 50 clinical samples such as gum scraping, tongue scraping, faeces, urine, nasal swabs; blood from 18 rinderpest infected cattle and 2 field samples. the comparative sensitivity of space, reverse phase passive haemagglutination and counter-immuno-electrophoresis tests was found to be 86.8, 84.5 and 79.8%, respectively.19873667851
a recent outbreak of rinderpest in east africa.rinderpest was brought under control in kenya in 1976 but in april 1986 an outbreak of the disease occurred in cattle in western kenya, five kilometres from the kenya-uganda border. this was the first confirmed field outbreak of the disease in kenya after a lull of over 10 years. clinical disease was confined to unvaccinated zebu calves aged six to eight months from which rinderpest virus was isolated. high titres of antibodies to rinderpest virus were demonstrated in sera collected from sheep a ...19873660452
production of monoclonal antibodies against rice dwarf virus and characterization of antigenic proteins with affinity media.hybridomas were developed which secreted antibodies to the core and outer capsids of rice dwarf virus (rdv). the reactive proteins of rdv were simply and rapidly analyzed using monoclonal antibody (moab)-coupled affi-gel 701 beads and sds polyacrylamide gel electrophoresis and were isolated with a moab-coupled sepharose 4b affinity column.19873654899
molecular cloning and sequence analysis of the rinderpest virus mrna encoding the hemagglutinin protein.we cloned the full-length cdnas corresponding to the mrna for the hemagglutinin (h) protein of rinderpest virus (rv) and determined the nucleotide sequence of rv-h. the gene of rv-h was composed of 1952 nucleotides and contained a single large open reading frame, which was capable of encoding a protein of 609 amino acids with a molecular weight of 68,330 da. the nucleotide sequence and predicted amino acid sequence were compared with those of the measles virus (mv)-h. the 5' end of the message ( ...19873629979
comparison of proteins induced in cells infected with rinderpest and peste des petits ruminants viruses.the two morbilliviruses rinderpest virus (rpv) and peste des petits ruminants virus (pprv) are closely related and cause severe disease in large and small ruminants, respectively. they show distinct epidemiological patterns and are distinguishable by reciprocal cross-neutralization tests. we have analysed the proteins induced by these viruses in infected cells and have shown that they can be distinguishable by a very marked difference in the apparent mol. wt. of the nucleocapsid (n) protein. the ...19873598564
simple and rapid dot-enzyme immunoassay for visual detection of rinderpest antibodies in bovine and caprine sera.a modified solid phase enzyme immunoassay (eia) is described for visual detection of anti-rinderpest virus (rpv) antibodies in cattle and goat sera. dots of rpv antigens were adsorbed to nitrocellulose (nc) paper (hence dot-eia) and the adsorptive reactive sites were blocked with skim milk powder. after immersion in bovine or caprine test serum bound antibodies were reacted with a peroxidase-conjugated anti-bovine or anti-caprine igg (h & l), respectively. positive reactions were easily visualiz ...19863544400
single radial hemolysis test for the detection of rinderpest antibody.the single radial hemolysis [srh] test was employed for detection of rinderpest antibodies in post-vaccinated serum samples as also in serum samples from animals recovered from rinderpest infection. the results were compared with counterimmunoelectrophoresis [cie] and serum neutralisation [sn] tests. the cie test was found to be more sensitive than srh but because of ease and simplicity srh can also be used for monitoring antibody development after vaccination.19863532467
application of a precipitinogen inhibition test in the detection of antibody to peste des petits ruminants virus. 19873444960
[successful development and methods of use of rinderpest vaccine]. 19873442452
an outbreak of peste des petits ruminants in a zoological collection.peste des petits ruminants virus was suspected to be the cause of a disease outbreak in a zoological collection at al ain in the arabian gulf. clinically the outbreak affected gazelles (gazellinae), ibex and sheep (caprinae) and gemsbok (hippotraginae); subclinical involvement of nilgai (tragelaphinae) was suspected. a morbillivirus was isolated and using monoclonal antibodies and biological tests in cattle, sheep and goats the virus of peste des petits ruminants was identified.19873424615
cloning and sequence analysis of the hemagglutinin gene of the virulent strain of rinderpest virus.we have cloned the cdna of the hemagglutinin (ha) gene of the virulent (kabete o) strain of rinderpest virus and produced a comparative analysis of its sequence with that of the ha genes of rinderpest (lapinized strain) and measles viruses. the gene has an open reading frame of 1827 nucleotides, and the derived protein is 609 amino acids long with a calculated molecular weight of 68,006. the kabete o ha polypeptide is identical in length to the ha of the lapinized strain of rinderpest virus and ...19883413986
cloning of the fusion gene of rinderpest virus: comparative sequence analysis with other morbilliviruses.we have cloned the cdna of the fusion (f) gene of the virulent (kabete o) strain of rinderpest virus and provided a comparative analysis of its sequence with that of the f genes of measles and distemper viruses. the gene has an open reading frame of 2241 nucleotides with two potential initiation codons in-frame. use of the first atg would produce a polypeptide 747 amino acids long with a calculated molecular weight of 81,068. however, we suggest that the second atg is used to generate the fo pro ...19883413983
antigenic variation between three strains of rinderpest virus detected by kinetic neutralisation and competition elisa using early rabbit antisera.strains of rinderpest virus (rv) with high and low virulence were compared with cell culture-attenuated rv for antigenic differences. sera collected from rabbits 1 week after inoculation with live virus distinguished between homologous and heterologous strains by kinetic neutralisation and competition elisa.19883376417
analysis of the polypeptides synthesized in rinderpest virus-infected cells.we have identified, by [35s]methionine labeling, eight major induced proteins and a number of minor proteins in rinderpest virus-infected bovine kidney cells. the polypeptides ranged in molecular weight from 212 to 21.5 kda. the majority of these polypeptides are virus specific, as demonstrated by immunoprecipitation with rabbit hyperimmune serum against rinderpest. infected cells radiolabeled with glucosamine contained a 75-kda polypeptide and a broad band migrating at 80 kda, both identified a ...19883354196
nucleotide sequence of segment s10 of the rice dwarf virus genome.dna complementary to the tenth largest (s10) of the 12 genome segments of rice dwarf virus (rdv) was cloned and its sequence was determined. this genome segment is 1319 nucleotides in length and has a single long open reading frame extending for 1059 nucleotides from the first aug triplet (residues 27 to 29). the predicted translation product comprises 352 amino acids and has a mol. wt. of 39094. rdv transcripts synthesized in vitro have the same polarity as the plus strand of the genome. termin ...19883335833
single and combined rinderpest vaccines. 19873314100
a reassessment of the dual vaccine against rinderpest and contagious bovine pleuropneumonia.in the light of the recent outbreaks of rinderpest in africa a further assessment of the efficacy of the simultaneous inoculation of rinderpest virus vaccine and contagious bovine pleuropneumonia vaccine was undertaken. groups of cattle were inoculated with a dual preparation of rinderpest vaccine virus and mycoplasma mycoides subspecies mycoides or m mycoides alone. these groups were then challenged with m mycoides, first unsuccessfully by an in-contact challenge method and then by subcutaneous ...19873300010
reverse phase passive haemagglutination test for the detection of rinderpest antigen.reverse phase passive haemagglutination [rpha] test was applied for the detection of rinderpest antigen in various organs of rinderpest infected cattle. the results of rpha were compared with counter immunoelectrophoresis [cie] and single radial haemolysis [srh] test. rpha was as sensitive as cie and srh in detecting rinderpest antigen.19873299936
fusion glycoprotein (f) of rinderpest virus: entire nucleotide sequence of the f mrna, and several features of the f protein.the full-length cdna corresponding to the mrna for the fusion protein of rinderpest virus (rv) was cloned and its complete nucleotide sequence was determined. the mrna for the f protein was composed of 2359 nucleotides and contained a single large open reading frame which was capable of encoding 566 amino acids with a molecular weight (mw) of 58,929. the rv-f mrna had a long noncoding region at the 5' end (586 bases) which was c-rich like the measles virus (mv)-f mrna but they did not appear to ...19883285575
experimental peste des petits ruminants (goat plague) in goats and sheep.in order to study the pathomorphology and immunohistochemistry of peste des petits ruminants, four goats and two sheep were inoculated intranasally with the malig-yemen strain of peste des petits ruminants virus. the animals developed fever, nasal discharge, oral erosions, cough and diarrhea. one goat and one sheep died and one moribund goat was killed. three animals survived the infection. at necropsy, erosive stomatitis, pneumonia and gastroenteritis were found. histopathologically the pneumon ...19883280108
induction of antinuclear antibodies in rabbits by infection with rinderpest viruses with different degree of virulence. 19883264353
structural proteins of rinderpest virus. ii: purification and characterization of the nucleocapsid protein n, the haemagglutinin protein, h and the matrix protein, m. 19883255671
economic analysis of the development and usage of thermoresistant rinderpest vaccine. 19883232645
prevalence of rinderpest antibodies in sheep and goats in southern india.rinderpest antibodies were demonstrated in 37 per cent of 2400 sheep and 29.5 per cent of 1000 goats in the southern states of india by the avidin-biotin enzyme linked immunosorbent assay technique. this technique, besides quantifying rinderpest antibodies, has provided a simple method for the differentiation of positive and negative serum samples on the basis of the development of a pink colour, a great advantage in the screening of large numbers of sera for field epidemiological studies. the p ...19883212917
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