Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| effects of infection with parainfluenza-3 virus and infectious bovine rhinotracheitis virus on neutrophil functions in calves. | calves were challenge exposed in separate experiments with parainfluenza-3 (pi-3) virus or infectious bovine rhinotracheitis (ibr) virus. blood neutrophils were assayed for functional activity every other day for at least 3 weeks by random migration, staphylococcus aureus ingestion, antibody-dependent cell-mediated cytotoxicity, cytochrome-c reduction, iodination, and native chemiluminescence. exposure to pi-3 virus resulted in a brief febrile response and no other clinical signs. alterations in ... | 1988 | 2840008 |
| effect of dietary selenium and vitamin e on the primary and secondary immune response in lambs challenged with parainfluenza3 virus. | this study was conducted to determine the effect of dietary selenium (se) and vitamin e (vit e) on various blood characteristics and the primary and secondary humoral immune response of lambs challenged with parainfluenza3 virus (pi3 v). treatments included: 1) +se/+vit e, 2) -se/+vit e, 3) +se/-vit e and 4) -se/-vit e. the basal diet (-se/-vit e) was deficient in se and vit e. sodium selenite (.2 mg se/kg diet) and alpha-tocopherol acetate (20 mg vit e/kg diet) were added to +se and +vit e diet ... | 1988 | 2840427 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the l protein. | the sequence of the gene encoding the l protein of the human parainfluenza 3 virus was determined by direct dideoxy sequence analysis of the genomic 50 s rna and confirmed by molecular cloning and sequence analysis of recombinant clones. a series of three overlapping clones was generated by primer extension using genomic 50 s rna as the template. these clones originate within the 5' end of the hemagglutinin-neuraminidase gene, span the entire l gene, and extend into the extracistronic 5' end of ... | 1988 | 2841798 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| current approaches to the development of vaccines effective against parainfluenza viruses. | infection of infants and children with parainfluenza viruses can lead to serious lower respiratory tract disease. in order to review new information on the genetic organization, epidemiology, and immunobiology of these viruses and of respiratory syncytial virus, who, in conjunction with the national institute of allergy and infectious diseases, held a workshop in bethesda, md, usa, 4-5 may 1987. new data on the immunobiology and epidemiology of human parainfluenza 3 virus were presented that sho ... | 1988 | 2844427 |
| levamisole-induced attenuation of alveolar macrophage dysfunction in respiratory virus-infected calves. | a luminol-amplified chemiluminescence (cl) assay was used to evaluate the effect of levamisole on the metabolic activity of pulmonary alveolar macrophages (pam) from parainfluenza-3 (pi-3) and infectious bovine rhinotracheitis (ibr) virus-infected calves. using selective beta-adrenergic agonists and antagonists, beta 1-adrenoceptors were shown to inhibit the pam reactive oxygen species-dependent cl response. beta 2 adrenoceptors were apparently not important in the regulation of cl in pam of con ... | 1988 | 2844684 |
| rabbit model for mucosal immunity in the bowel: i. establishment of virus-infected ileal loops. | an animal model was designed for use in studies of initial cellular immune responses to virus infection of the intestinal mucosa. the animal chosen was the new zealand white rabbit and the mucosal site the subterminal ileum, isolated in a thirty-vella loop. the antigen used was parainfluenzavirus type 3, which would normally be destroyed by bile salts if ingested. loops approximately 20 cm in length, each containing at least one peyer's patch, were exteriorised through left paramedian stomata. a ... | 1988 | 2844978 |
| immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with bhv1 and pi3 viruses. | peripheral blood mononuclear cells (pbmc) from calves infected with bovine herpesvirus type 1 (bhv1) or parainfluenza 3 virus (pi3) were cultured in vitro in the presence of inactivated specific antigen presented on mdbk cells. in the presence of inactivated antigen, pbmc from both bhv1-infected and control calves produced interferon (ifn)-alpha in 24 hour cultures. altering the culture conditions did not result in the detection of immune-specific ifn produced by mononuclear cells from bhv1-infe ... | 1988 | 2848301 |
| interactions of a nonneutralizing igm antibody and complement in parainfluenza virus neutralization. | while many viruses activate the complement cascade directly, this is generally not a neutralizing event in the absence of antibody. we used a nonneutralizing igm monoclonal antibody to parainfluenza virus type 3 (piv3) hemagglutinin-neuraminidase (hn) to explore the role of antibody in complement-dependent neutralization of piv3. neither the antibody nor nonimmune guinea pig serum (gps) neutralized piv3 significantly, but a more than 100-fold reduction in titer was found when antibody and gps we ... | 1988 | 2849234 |
| antibodies to parainfluenza virus type 3 in minnesota swine. | 1988 | 2849496 | |
| [etiology of pneumonia in children]. | 1988 | 2851127 | |
| [serologic survey of rinderpest, ibr-ipv, rsb, pi3 and bvd-md viruses in cattle in shaba and western zaire]. | 1988 | 2851152 | |
| effect of respiratory infections caused by bovine herpesvirus-1 or parainfluenza-3 virus on bovine alveolar macrophage functions. | calves, 90 to 130 days old, were inoculated with bovine herpesvirus-1 (bhv-1) or parainfluenza-3 (pi-3) virus. pulmonary lavage specimens obtained from calves before virus inoculation contained 98% alveolar macrophages (am) and 1% neutrophils. six days after inoculation, the mean percentage of neutrophils in lavage specimens had significantly increased to 7.9 +/- 6.0% in bhv-1-inoculated calves and to 18.3 +/- 9.9% in pi-3 virus-inoculated calves, reflecting viral-induced pulmonary inflammation ... | 1988 | 2851951 |
| fatal pneumonia caused by parainfluenza type 3 virus. | 1988 | 2854685 | |
| bovine parainfluenza-3 virus proteins recognized by antibodies of aerosol-exposed calves. | bovine parainfluenza-3 (bpi-3) virus proteins were radiolabeled with [35s]methionine. control serum containing antibodies to bpi-3 virus obtained from calves that had been immunized by aerosol exposure and by im inoculation and sera of cattle that were exposed to bpi-3 virus by aerosol only were used to immunoprecipitate bpi-3 virus proteins. the immunoprecipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. viral proteins with estimated molecular weights of 72,00 ... | 1988 | 2854712 |
| parainfluenza 3 infections. | 1988 | 2854823 | |
| [pathogenicity of parainfluenza virus type 3 in mice]. | 1988 | 2855381 | |
| differences in bovine parainfluenza 3 virus variants studied by sequencing of the genes of viral envelope proteins. | by determining gene nucleotide sequences we compared the primary structures of the membrane (m), fusion (f), and hemagglutinin-neuraminidase (hn) proteins of bovine parainfluenza 3 virus strains, m, sc, and mr which are substrains derived from a wild strain yn. the m and sc viruses are indistinguishable in having very weak hemagglutination (ha) and neuraminidase (na) activities, but m virus' syncytium-inducing (si) activity is considerably higher than that of the sc virus. however, the results s ... | 1988 | 2829426 |
| parainfluenza virus type 2 haemagglutinin-neuraminidase glycoprotein characterized with monoclonal antibodies. | thirteen monoclonal antibodies (mabs) were prepared against human parainfluenza virus type 2 (piv2). these mabs reacted with the haemagglutinin-neuraminidase glycoprotein with an mr of 84k. the mabs defined one antigenic site which could be divided into five epitopes. a correlation between haemagglutination inhibition (hi) and neutralization activity could be seen although one mab, which recognized a distinct epitope, showed neutralization and no hi activity to piv2. the reactivity of the mabs w ... | 1988 | 2451708 |
| contribution to laboratory diagnosis of mumps and parainfluenza. | specific igm and igg antibodies to mumps virus (mv) were detected in sera of mumps-patients by elisa in agreement with the results obtained by indirect immunofluorescence (if). of given sera 37.5% contained igm reacting in indirect elisa also with the antigens of parainfluenza virus (piv) t3. in all patients with respiratory illness over 2 years of age, the significant increase of antibodies to piv in haemagglutination inhibition (hi) test was in good correlation with serum igm and igg antibody ... | 1988 | 2906222 |
| molecular cloning and characterization of six genes, determination of gene order and intergenic sequences and leader sequence of mumps virus. | mrna isolated from mumps virus-infected vero cells was converted into cdna and cloned into the psti site of the plasmid pbr322. after screening with 32p-labelled cdna synthesized from poly(a)+ rna of uninfected or mumps virus-infected vero cells, five different groups of virus-specific clones were obtained. the virus specificity of the clones was confirmed by northern blot analysis, in which the cdna inserts from the five different groups hybridized to mrnas of about 2100, 1500, 1450, 2000 and 2 ... | 1988 | 3183630 |
| sequence determination of the mumps virus hn gene. | the hemagglutinin-neuraminidase protein (hn) of mumps virus was purified by immunoaffinity chromatography and fragmented by the combined action of cnbr and trypsin. the resulting peptides were separated by hplc and sequenced by automated edman degradation. using this hn-specific amino acid sequence data, a degenerate oligonucleotide was produced and subsequently used to screen a mumps virus cdna library to isolate hn-specific clones. the complete nucleotide sequence of the hn gene was determined ... | 1988 | 3369084 |
| immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. | antigenic interrelationships among paramyxoviruses were examined by immunoprecipitation of isotope-labelled virus-infected cell lysates with specific antisera against virions or virus components. sendai virus, human parainfluenza virus type 1 (pa-1) and human parainfluenza virus type 3 (pa-3) belonged to one antigenic group, and human parainfluenza virus type 2 (pa-2), human parainfluenza virus type 4 (pa-4), mumps virus (muv) and simian virus 5 to a second group. furthermore, the human paramyxo ... | 1987 | 3572366 |
| sequence analysis of the matrix protein gene of human parainfluenza virus type 3: extensive sequence homology among paramyxoviruses. | the sequences of the human parainfluenza virus type 3 (piv3) matrix (m) mrna [1150 nucleotides exclusive of poly(a)] and predicted m protein (353 amino acids) were determined by sequence analysis of cloned cdna and viral genomic rna. the gene-end sequence of the m gene differed from the semi-conserved gene-end sequence of the other piv3 genes by an apparent insertion of eight nucleotides. the piv3 m protein shared high sequence homology with sendai virus and moderate homology with measles virus ... | 1987 | 2883252 |
| pasteurella haemolytica serotype 1 colonization of the nasal passages of virus-infected calves. | nasal passages of calves with a virus-induced respiratory tract disease became colonized by pasteurella haemolytica serotype 1 after they were inoculated intranasally with p haemolytica. inoculation with infectious bovine rhinotracheitis virus caused a more severe clinical illness and resulted in a greater degree of colonization with p haemolytica than developed after inoculation with parainfluenza-3 virus. nasal passages of parainfluenza-3 virus-inoculated calves were colonized to a greater deg ... | 1987 | 2829666 |
| complete nucleotide sequence of the matrix protein mrna and three intergenic junctions of human parainfluenza virus type 3. | the complete sequence of the gene encoding the matrix protein (m) of human parainfluenza virus type 3 (piv-3) was determined from cdna clones and from primer extension dideoxy sequencing of the viral genome. the m mrna is 1150 nucleotides in length, exclusive of polyadenylate, and codes for a protein of 353 amino acids, having a calculated molecular weight of 39,480. the m protein of piv-3 was found to have a high degree of sequence homology with that of a closely related paramyxovirus, sendai v ... | 1987 | 3027966 |
| efficiency of immunofluorescence for rapid detection of common respiratory viruses. | rapid immunofluorescence (fa) methods for the detection of common respiratory viruses were compared with culture results over a 3-year period to assess the relative efficiency of fa in a clinical laboratory setting. for respiratory syncytial virus, efficiencies were high (sensitivity, 90 to 95%; specificity, 92 to 95%). the sensitivity of fa for detection of parainfluenza virus type 1, parainfluenza virus type 3, influenza a virus, and adenoviruses ranged from 28 to 63%, but specificities for th ... | 1987 | 3029168 |
| early testing of cell cultures for detection of hemadsorbing viruses. | hemadsorption of primary monkey kidney cell cultures was commenced at 1 day after inoculation to evaluate how rapidly influenza a virus, influenza b virus, and parainfluenza virus types 1, 2, and 3 could be detected by this method from respiratory specimens. overall, 38% of all isolates could be detected within 24 h of inoculation, and 69% could be detected within 48 h. all influenza a viruses and all but one influenza b virus were detected by day 3. | 1987 | 3029173 |
| glycoproteins of human parainfluenza virus type 3: affinity purification, antigenic characterization and reconstitution into lipid vesicles. | monoclonal antibodies to the envelope glycoproteins, hn and f, of human parainfluenza virus type 3 were coupled to a sepharose 4b matrix and used for affinity purification of the viral glycoproteins. the purity of the glycoproteins was demonstrated by sds-page followed by fluorography or silver staining. the antigenicity of the glycoproteins was determined by immunization of rabbits; polyclonal rabbit antisera demonstrated inhibition of functional activities of the virus glycoproteins. the f gly ... | 1987 | 3029283 |
| diseases of wapiti utilizing cattle range in southwestern alberta. | specimens from 28 wapiti (cervus elaphus canadensis) were collected by hunters in southwestern alberta in 1984. various tests were performed to detect infections and conditions that could affect cattle sharing the range or cause disease in wapiti. serum antibodies were present against leptospiral serovars autumnalis (25%), bratislava (4%), and icterohaemorrhagiae (8%), and the viruses of bovine virus diarrhea (52%), infectious bovine rhinotracheitis (45%), and parainfluenza type 3 (13%). no sero ... | 1987 | 3029443 |
| cell fusion in viral diseases. | 1987 | 3029598 | |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus gene encoding the matrix protein. | the sequence of the matrix (m) protein gene and contiguous intergenic regions of the human parainfluenza 3 virus (pf3) was determined by molecular cloning. the encoded m protein contains 354 amino acids and has a predicted mol wt of 39,506. the m protein amino acid sequence was compared to the homologous proteins from other members of the paramyxoviridae family. the pf3 protein shared 61% homology with the sendai virus protein and approximately 35% homology with measles and canine distemper viru ... | 1987 | 3029963 |
| parainfluenza type 3 in newborns. | 1987 | 3031576 | |
| nucleotide sequence of the bovine parainfluenza 3 virus genome: its 3' end and the genes of np, p, c and m proteins. | we present the nucleotide sequence of bovine parainfluenza 3 virus (bpiv3) genome from its 3' end to the opening region of the f gene, through the np, p plus c, and m genes. comparison of the sequence with those reported for other paramyxoviruses indicated that bpiv3 was most similar to human parainfluenza 3 virus (hpiv3), and also very similar to sendai virus in the structural make-up of its genome and the amino acid sequences of its gene products, suggesting that these three viruses constitute ... | 1987 | 3031614 |
| nucleotide sequence of the bovine parainfluenza 3 virus genome: the genes of the f and hn glycoproteins. | by analysing complementary dna clones constructed from genomic rna of bovine parainfluenza 3 virus (bpiv3), we determined the nucleotide sequence of the region containing the entire f and hn genes. their deduced amino acid sequences showed about 80% homologies with those of human parainfluenza 3 virus (hpiv3), about 45% with those of sendai virus, and about 20% with those of sv5 and newcastle disease virus (ndv), indicating, together with the results described in the preceding paper on the np, p ... | 1987 | 3031615 |
| nucleotide sequence of the human parainfluenza virus 3 matrix protein gene. | 1987 | 3031622 | |
| alveolar macrophage phagocytic kinetics following pulmonary parainfluenza-3 virus infection. | qualitative and quantitative evaluations of the cellular components of bronchoalveolar washings of calves with experimental parainfluenza-3 virus pneumonitis and control calves were made. calves were exposed to 10(9) tcid50 of pi-3 by intranasal aerosol exposure and bronchoalveolar cells obtained 7 days after infection by volume-controlled bronchopulmonary lavage. transient tachypnea and pyrexia occurred in all infected calves, and virus was recoverable at 7 days from nasal swabs and lung tissue ... | 1987 | 3033112 |
| nucleotide sequence of the coding and flanking regions of the human parainfluenza virus type 3 fusion glycoprotein gene. | the complete nucleotide sequence of the human parainfluenza virus type 3 (hpiv3) fusion (f) protein gene has been determined. the hpiv3 f gene is 1851 nucleotides long including six u residues in the genomic rna, which probably direct synthesis of the first few nucleotides in the f mrna polyadenylate tail. the hpiv3 f gene contains a single long open reading frame coding for 539 amino acids. the predicted molecular weight of the unglycosylated precursor f0 protein was 60031. four potential carbo ... | 1987 | 3033123 |
| a case of rhabdomyolysis in chronic renal failure. | rhabdomyolysis can be induced by a variety of physical and chemical insults to skeletal muscle. though there are only a few reports of myositis associated with viral diseases, and no reported cases of virus-induced rhabdomyolysis in uremic patients. chronic uremic states have been known to potentiate a variety of metabolic and immunological abnormalities. present case had an acute, progressive and fatal rhabdomyolysis which was thought to be induced by virus infection. a 43-year-old man had rece ... | 1987 | 3033362 |
| giant cell pneumonia caused by parainfluenza type 3 in a patient with acute myelomonocytic leukemia. | a 4 1/2-year-old boy with acute myelomonocytic leukemia developed fever, neutropenia, and a prolonged respiratory illness while receiving maintenance chemotherapy. an open lung biopsy specimen demonstrated a giant cell pneumonia with intracytoplasmic and probable intranuclear viral inclusions of the paramyxovirus type. serologic studies demonstrated convincing evidence of a parainfluenza type 3 infection. although parainfluenza type 3-induced giant cell pneumonia has been reported in infants wit ... | 1987 | 3034189 |
| respiratory virus infection of peripheral blood monocytes: correlation with ageing of cells and interferon production in vitro. | the ability of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) to replicate in peripheral blood monocytes cultured in vitro for 1, 2, 4 or 7 days prior to infection was investigated. inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. piv3 induced extensive syncytium formation, whereas rsv caused a cytopathic effect manifest by increased rounding of the cells with minimal syn ... | 1987 | 3035067 |
| defective interfering particles of human parainfluenza virus 3. | a cyclic pattern of virus production was observed when human parainfluenza virus 3 (hpiv3) was serially passaged nine times in llc-mk2 cells. viruses produced from serial passages 8 and 9 interfered with the replication of standard hpiv3. three subgenomic rna species (di-1, di-2, and di-3) and virus genomic rna were detected in the progeny virions produced from cells mixedly infected with standard virus and virus from either serial passages 5 or 8. northern blot analysis with probes representing ... | 1987 | 3035791 |
| [proteins of the human parainfluenza virus]. | proteins of human parainfluenza type 3 virus propagated in green monkey kidney cultures are described. the presence of 4 major and 3 minor polypeptides is described along with their molecular weights. protein f1 was found as a double band, its both components having a similar intensity. only the upper band of f1 protein was found in the virus grown in vero cell culture which has a low sensitivity for this virus. fractionation of the virus and analysis of the proteins of subviral structures were ... | 1987 | 3037795 |
| antigenic analysis of human and bovine parainfluenza virus type 3 strains with monoclonal antibodies. | the antigenic characteristics of eight human strains and two bovine strains, one of which is represented by two plaque variants, of parainfluenza virus type 3 were analysed. the strains and variants were compared using 52 monoclonal antibodies against five, two, six and six epitopes of the haemagglutinin-neuraminidase (hn), fusion, nucleocapsid and matrix viral proteins respectively, employing radioimmuno-precipitation and immunofluorescence assays. the human strains, seven of which were isolate ... | 1987 | 3039045 |
| [experiences with the riems vaccine combination pi-3 and riems ibr/ipv vaccines in cattle-raising stock]. | 1987 | 2831841 | |
| [immune response of calves following vaccination with paramyxovirus parainfluenzae-3 and adenovirus-1 under different colostral immunity stresses]. | studied was the immune response of calves, with colostrum antibodies and without colostrum antibodies, immunised with different dosages of live bivalent vaccine against p. parainfluenzae-3 and adenovirus-1. for the group of calves with colostrum immunity, after single hypodermic immunisation with doses from 10(4) till 10(5) cpe50/cm3 for adenovirus, was not proved immune response, as a result of blocking of the vaccinal antigen of colostrum antibodies. for calves which missed these antibodies, t ... | 1987 | 2833005 |
| antigenic and structural properties of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3: sequence analysis of variants selected with monoclonal antibodies which inhibit infectivity, hemagglutination, and neuraminidase activities. | the hemagglutinin-neuraminidase (hn) gene sequence was determined for 16 antigenic variants of human parainfluenza virus type 3 (piv3). the variants were selected by using monoclonal antibodies (mabs) to the hn protein which inhibit neuraminidase, hemagglutination, or both activities. each variant had a single-point mutation in the hn gene, coding for a single amino acid substitution in the hn protein. operational and topographic maps of the hn protein correlated well with the relative positions ... | 1987 | 2437318 |
| immediate persistent infection by human parainfluenza virus 3: unique fusion properties of the persistently infected cells. | we describe here a persistently infected cell system with unique properties. cells infected with human parainfluenza virus type 3 (pf3) at high multiplicities of infection showed little or no cytopathic effects (cell fusion). unlike other paramyxovirus persistent infections that require a long development time, the majority of the cells survived the initial infection and formed persistently infected cell cultures that were immediately available for study. in addition, unlike other paramyxovirus ... | 1987 | 2438380 |
| enhancement by parainfluenza 3 infection of contractile responses to substance p and capsaicin in airway smooth muscle from the guinea pig. | guinea pigs were inoculated by nasal insufflation with parainfluenza 3 (p-3) or virus growth medium 4 days before performing in vitro pharmacologic studies on left bronchial ring segments. cumulative dose-response studies with capsaicin revealed an enhanced contractile response after p-3 infection. the sensitivity and magnitude of contractile effects of substance p in the left bronchi were also enhanced by p-3 infection. after pretreatment of the isolated tissues with phenoxybenzamine to block h ... | 1987 | 2443048 |
| [study of the multiplication in cell cultures of two strains of para-influenza virus type 3. iii. multiplication in bhk 21 cells]. | study was conducted on the multiplication of two strains (c243 and d) of parainfluenza virus type 3 in bhk 21 cells. multiplication curve of the virus was established and immunohistochemical aspects of the process were investigated. chronological study of successive steps of the formation and development of viral components allowed to see that the virus multiplication rate is low in this cell system. the parainfluenza antigen became detectable by immunofluorescence in the infected cell perinucle ... | 1987 | 2444030 |
| [effect of tuberculin and levamisole on the immune response after vaccinating calves against para-influenza and salmonella infections]. | studied was the effect of tuberculin and levamizole on the immune response following vaccination of calves against parainfluenza and salmonella infections. it was found that in calves that had colostrum antibodies against parainfluenza the immunogenic stimulation with the two agents first led to a drop of the total protein and of the antibody titer of the blood serum; in later periods the antibody titer grew severalfold. the stimulation with tuberculin and levamizole in the case of salmonella in ... | 1987 | 2820117 |
| structural proteins of bovine parainfluenza-3 virus. | six structural proteins of bovine parainfluenza-3 virus (pi-3v) labeled with [35s]-methionine could be resolved by polyacrylamide gel electrophoresis (page). five structural proteins of this virus had been previously reported. the 6 proteins found in this study were: l, a 180,000 (180 kd) molecular weight (mw) large protein; p, 83 kd phosphoprotein; hn, 69 kd hemagglutinin-neuraminidase glycoprotein; np, 66 kd nucleocapsid protein; f, 55 kd fusion glycoprotein; and m, 38 kd matrix protein. selec ... | 1987 | 2821671 |
| expression of biologically active and antigenically authentic parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein by a recombinant baculovirus. | the hemagglutinin-neuraminidase (hn) gene of human type 3 parainfluenza virus has been inserted into a baculovirus expression vector under the control of the polyhedrin promoter. hn protein produced in insect cells by the recombinant baculovirus appeared to be glycosylated, was transported to the cell surface, and was biologically active. all of the hn epitopes previously mapped functionally to a region(s) involved in neuraminidase and/or hemagglutination activities were conformationally unalter ... | 1987 | 2821684 |
| nucleotide sequence of the coding and flanking regions of the human parainfluenza virus 3 hemagglutinin-neuraminidase gene: comparison with other paramyxoviruses. | the nucleotide sequence of the human parainfluenza virus 3 (hpiv3) hemagglutinin-neuraminidase (hn) gene has been determined using cdna clones derived from both hpiv3 genomic rna and mrna. the hn mrna contains 1,882 nucleotides, not including the poly(a) tail. primer extension experiments were carried out to locate the 5' terminal nucleotide of the hn mrna. the 3' end of the mrna was located at a putative polyadenylation signal. the hpiv3 hn mrna has one large open reading frame that codes for 5 ... | 1987 | 2822598 |
| respiratory viruses in children admitted to hospital in edinburgh 1972-1985. | the temporal incidence of respiratory viruses identified in children admitted to an edinburgh hospital over a period of 14 years, 1972-1985, is described, respiratory syncytial (rs) virus was identified most often, usually (but not in 1979 and 1985) with single peaks of activity in the winter months. influenza virus infections were seen in the winter and spring but we failed to find evidence of interference between rs and influenza virus. of the viruses studied, the parainfluenza viruses were th ... | 1987 | 2822808 |
| expression of the f and hn glycoproteins of human parainfluenza virus type 3 by recombinant vaccinia viruses: contributions of the individual proteins to host immunity. | cdna clones containing the complete coding sequences for the human parainfluenza virus type 3 (piv3) fusion (f) and hemagglutinin-neuraminidase (hn) glycoprotein genes were inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of the p7.5 early-late vaccinia virus promotor. the recombinant vaccinia viruses, designated vaccinia-f and vaccinia-hn, expressed glycoproteins in cell culture that appeared to be authentic with respect to glycosylation, disulfide linkage ... | 1987 | 2822951 |
| [use of an immune blotting method (western) for studying viral antigens and for detecting antibodies to viral proteins]. | the technique of the procedure and the results of the use of immune ("western") blotting method for studies on viral antigens and detection of antibodies to individual proteins of viral particles are described. the possibility of detection and study of individual viral antigens in the whole plasma of patients or carriers is demonstrated by the example of hbs antigen of human hepatitis b virus. the method of immune blotting was used for screening of human sera for the detection of antibodies to t ... | 1987 | 2823482 |
| field trial of a quadrivalent vaccine against calf respiratory disease. | a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma dispar and m bovis, emulsified with an oil adjuvant, was tested for efficacy against naturally occurring calf respiratory disease. three batches of beef cattle aged 12, seven and three weeks at the time of first vaccination were used. within each batch of approximately 100 animals, half were vaccinated subcutaneously on three occasions, three weeks apart and half served as ... | 1987 | 2825397 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus genes encoding the surface glycoproteins, f and hn. | the sequence of the genes encoding the fusion (f) and hemagglutinin-neuraminidase (hn) glycoproteins of the human parainfluenza 3 virus was determined by molecular cloning. the genes were cloned by primer extension using genomic 50 s rna as the template. a series of four overlapping clones was generated from the 3' end of the fusion gene which extended across the gene end and intergenic boundaries of the f-hn and hn-l genes. the f gene extends 1851 nucleotides (inclusive of the putative transcri ... | 1987 | 2825443 |
| prevalence of antibodies to some viral pathogens, brucella abortus and toxoplasma gondii in serum from camels (camelus dromedarius) in sudan. | 1987 | 2825450 | |
| [progress of the campaign against acute respiratory viral diseases in children]. | 1987 | 2826029 | |
| [infectious respiratory tract diseases in cattle in the winter of 1985/86: detection of infection by bovine respiratory syncytial virus and parainfluenza virus type 3 by immunofluorescence in nasal swab samples and by antibody titer increase in serum (elisa)]. | 1987 | 2827303 | |
| protection against respiratory disease in calves induced by vaccines containing respiratory syncytial virus, parainfluenza type 3 virus, mycoplasma bovis and m dispar. | a field trial to assess the ability of two vaccines to protect calves against respiratory disease was carried out on a large beef rearing unit in southern england over the two winters of 1983 to 1984 and 1984 to 1985. a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma bovis and m dispar or a vaccine containing only the respiratory syncytial virus component were inoculated into 246 and 245 calves, respectively; 245 calves r ... | 1987 | 2827366 |
| monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. | a panel of twenty monoclonal antibodies to the hemagglutinin-neuraminidase (hn) glycoprotein of human parainfluenza 3 (pi3) virus has been obtained and tentatively classified into four different groups based on reactivity in hemagglutination inhibition (hi), neuraminidase inhibition (ni), and plaque neutralization (nt) tests. the antibodies were tested for cross-reactivity with bovine pi3 virus, sendai virus, and simian virus 5 (sv5). only two of these antibodies showed similar reactivities with ... | 1986 | 2417412 |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus rna encoding the nucleocapsid protein. | the sequence of 1690 nucleotides from the 5' end of the viral complementary rna for the human parainfluenza 3 virus was determined by molecular cloning. one large open reading frame consisting of 1548 nucleotides was demonstrated. the encoded protein, the nucleocapsid protein (np), consists of 515 amino acids, and has a predicted molecular weight of 57,819. a noncoding 5' sequence of 51 nucleotides is present at the end of the np-mrna. two consensus sequences were identified which are homologous ... | 1986 | 2418584 |
| characterization of four parainfluenza virus type 3 proteins by use of monoclonal antibodies. | monoclonal antibodies directed against four structural components of the atcc strain c243 of parainfluenza virus type 3 were produced. the specific reaction of the antibodies with individual structural components was determined by radioimmune precipitation assay. in the collection of monoclonal antibodies, 21 reacted with the haemagglutinin-neuraminidase (hn) glycoprotein (mol. wt. 72,000), eight with the fusion (f) glycoprotein (mol. wt. 64,000), 27 with the nucleocapsid (np) protein (mol. wt. ... | 1986 | 2426392 |
| [a case of myocardial infarction in an 11-year-old girl with myocarditis]. | 1986 | 3035474 | |
| complete sequence of the major nucleocapsid protein gene of human parainfluenza type 3 virus: comparison with other negative strand viruses. | the sequence of the major nucleocapsid protein (np) mrna and its encoded protein were deduced by sequencing a cdna clone representing the complete mrna. the cdna sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic rna by primer extension using synthetic oligonucleotides. the np mrna contains 1,641 nucleotides exclusive of poly(a) and encodes an np protein of 515 amino acids. alignment of the human parainfluenza type 3 virus (pf3) np protein sequence with that of send ... | 1986 | 2878059 |
| parainfluenza virus bronchiolitis. epidemiology and pathogenesis. | an investigation of the epidemiology and pathogenesis of bronchiolitis due to parainfluenza virus (pv) was carried out. bronchiolitis due to pv occurred most commonly in non-caucasian males. breast-fed infants exhibited a reduced risk of developing bronchiolitis. once an episode of pv bronchiolitis occurred, both exposure to cigarette smoke and bottle feeding were associated with an increased frequency of recurrent wheezing, and subsequent infection with respiratory virus almost uniformly result ... | 1986 | 3002169 |
| human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mrna and limited amino acid sequence of the purified protein. | the nucleotide sequence of mrna for the hemagglutinin-neuraminidase (hn) protein of human parainfluenza type 3 virus obtained from the corresponding cdna clone had a single long open reading frame encoding a putative protein of 64,254 daltons consisting of 572 amino acids. the deduced protein sequence was confirmed by limited n-terminal amino acid microsequencing of cnbr cleavage fragments of native hn that was purified by immunoprecipitation. the hn protein is moderately hydrophobic and has fou ... | 1986 | 3003381 |
| protection of weanling hamsters from experimental infection with wild-type parainfluenza virus type 3 (para 3) by cold-adapted mutants of para 3. | parainfluenza virus type 3 (para 3) was adapted to replicate at 20 degrees c, a nonpermissive temperature for wild-type (wt) para 3. serial passage at 20 degrees c resulted in the generation of cold-adapted (ca) and temperature-sensitive (ts) mutants. these mutant viruses have been characterized both in vitro and in vivo [belshe and hissom (1982): journal of medical virology 10:235-242; crookshanks and belshe (1984): journal of medical virology 13:243-249]. we now report the evaluation of three ... | 1986 | 3005486 |
| occurrence of antibodies to the etiologic agents of infectious bovine rhinotracheitis, parainfluenza 3, leptospirosis, and brucellosis in white-tailed deer in minnesota. | a serologic survey was conducted on 628 white-tailed deer (odocoileus virginianus) in 1976 and 1979-1980. tests for antibodies to the etiologic agents of infectious bovine rhinotrancheitis (ibr), parainfluenza 3 (pi3), leptospirosis, and brucellosis produced positive results of 15%, 20%, 3% and 0%, respectively. adult deer had significantly higher prevalence of antibodies to ibr virus and pi3 virus than fawns. these data provide a basis for monitoring these disease agents in minnesota's white-ta ... | 1986 | 3005666 |
| antibodies to bovine bacterial and viral pathogens in pronghorns in alberta, 1983. | sera from 210 pronghorns (antilocapra americana) ranging in southeastern alberta were tested for antibodies to disease agents present in indigenous cattle. no antibodies to brucella abortus, leptospira interrogans serovars pomona, hardjo, or grippotyphosa, or infectious bovine rhinotracheitis virus were found. antibodies at prevalences of 43.8% and 49.2% were detected to bovine virus diarrhea (bvd) and parainfluenza type 3 (pi-3) viruses, respectively. the much higher prevalence of bvd virus ant ... | 1986 | 2845155 |
| [varying nature of the cell receptors for influenza and para-influenza viruses]. | a comparative study of receptors for influenza virus, fowl plague virus, and human parainfluenza type 3 virus was carried out. natural receptors of guinea pig erythrocytes were destroyed with neuraminidase, and individual gangliosides gm1, gd1a, and gt1b were inserted into their membranes. the labeled virus was adsorbed on the erythrocytes modified in this manner, and the degree of restoration of the receptor activity of erythrocytes lost after neuraminidase treatment was determined. two ganglio ... | 1986 | 3008439 |
| [structural organization and proteins of the human type-3 para-influenza virus]. | the structure of human parainfluenza type 3 virus was studied by electron microscopy and virion fractionation by treatment with a detergent and high ionic strength. the protein spectrum of the virus was studied. the presence of 6 structural proteins was revealed of which two, hn and f, are glycoproteins. intracellular cleavage of f0 protein into f1+2 proteins was demonstrated in a pulse-chase experiment. a tighter binding of hn protein than of f protein with the virus lipoprotein membrane was ob ... | 1986 | 3008440 |
| effect of recombinant dna-derived bovine and human interferons on replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses. | antiviral effects of recombinant dna-derived bovine (bo) and human (hu) interferons (ifn) on the replication of bovine herpesvirus-1, parainfluenza-3, and respiratory syncytial viruses were studied. bovine monolayer cultures were treated with recombinant dna-produced bo ifn-alpha 1, bo ifn-beta 2, hu ifn-alpha a, or hu ifn-alpha a/d and then challenge exposed with bovine herpesvirus-1, bovine parainfluenza-3 virus, bovine respiratory syncytial virus, or vesicular stomatitis virus. treatment with ... | 1986 | 3008606 |
| rapid diagnosis of respiratory virus infections in patients with acute respiratory disease. | viral respiratory infections represent a significant segment of the total respiratory disease spectrum; however, until recently the laboratory diagnosis of viral respiratory infections was relatively inefficient. development of new and improved immunologic assay systems has paved the way for accurate and reliable rapid diagnostic tests that detect viral antigens in clinical specimens. we conducted a careful and elaborate study in which radioimmunoassay for antigen detection was compared with a b ... | 1986 | 3009083 |
| the effect of parainfluenza virus type 3 on the phagocytic cell response of the ovine lung to pasteurella haemolytica. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica 4 to 6 days after the inoculation of parainfluenza virus type 3 (pi3). some were killed immediately (0 h) and others 24 h later. control groups were inoculated with pi3 alone, p. haemolytica alone or media alone. pulmonary phagocytic cells, p. haemolytica and pi3 were recovered by pulmonary lavage. the phagocytes were separated into alveolar macrophage (am) and neutrophil ... | 1986 | 3010545 |
| 3'-terminal sequence of human parainfluenza virus 3 genomic rna. | 1986 | 3012476 | |
| conserved structures among the nucleocapsid proteins of the paramyxoviridae: complete nucleotide sequence of human parainfluenza virus type 3 np mrna. | the nucleotide sequence of the mrna coding for the nucleocapsid protein (np) of the paramyxovirus, human parainfluenza virus type 3 (piv-3), has been determined. the np mrna was found to contain 1642 bases, excluding poly(a), and encode a protein of 515 amino acids, with a molecular weight of 57,823. amino acid residues 1 through 420 of piv-3 np protein showed extensive sequence homology with the corresponding amino acids of sendai virus nucleocapsid protein. there was virtually no homology betw ... | 1986 | 3012866 |
| fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. | the complete sequences of the fusion (f) mrna and protein of human parainfluenza virus type 3 (pf3) were determined from overlapping cdna clones. to confirm the cdna sequence, the complete sequence of the f gene was determined independently by dideoxynucleotide sequencing of genomic rna using synthetic oligonucleotide primers. the mrna contains 1845 nucleotides, exclusive of poly (a), has an unusually long (193-nucleotide) 5' nontranslated region, and encodes an f0 protein of 539 amino acids. th ... | 1986 | 3012869 |
| enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. | an enzyme linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against infectious bovine rhinotracheitis (ibr), parainfluenza-3 (pi3), adenovirus type 3 (adeno 3) and bovine respiratory syncytial (brs) viruses. paired serum samples from calves vaccinated with live attenuated virus vaccines were tested. the elisa compared favorably with the virus neutralization test for detecting serologic responses to ibr, brs, and adeno 3 viruses or with the hemagglutination inhib ... | 1986 | 3014711 |
| human parainfluenza virus type 3: messenger rnas, polypeptide coding assignments, intergenic sequences, and genetic map. | cdna clones of mrnas for the major nucleocapsid protein (np), the nucleocapsid p protein plus the nonstructural c protein (p+c), and the matrix protein (m) of human parainfluenza virus type 3 (pf3) were identified by hybrid arrest and hybrid selection of in vitro translation. previously, cdna clones were identified and sequenced for the hemagglutinin-neuraminidase glycoprotein (hn) and the fusion glycoprotein (f) mrnas (n. elango, j. e. coligan, r. c. jambou, and s. venkatesan, j. virol. 57:481- ... | 1986 | 3016327 |
| messenger rna encoding the phosphoprotein (p) gene of human parainfluenza virus 3 is bicistronic. | the complete nucleotide sequence of the phosphoprotein (p) mrna of human parainfluenza virus 3 (piv-3) was derived from two cdna clones spanning almost the entire p gene. the mrna, excluding the poly(a) tail, is 2014 nucleotides long and is bicistronic. the first open reading frame (orf) codes for the phosphoprotein (p) of mol wt 68,860. seven nucleotides downstream from the first aug codon, in a +1 reading frame, there is an additional orf which can code for a polypeptide of mol wt 23,266. the ... | 1986 | 3016995 |
| induction of immunity against pneumonic pasteurellosis following experimental infection in calves. | immunity against pneumonic pasteurellosis was studied in calves after recovery from experimental respiratory disease with pasteurella haemolytica. nine calves were exposed to aerosols of parainfluenza-3 virus and pasteurella haemolytica a1 six days apart to produce respiratory disease. after recovery from the disease, these nine principal and four control calves were challenged with aerosols of bovine herpesvirus 1 and p. haemolytica a1 four days apart. with this viral-bacterial challenge, the n ... | 1986 | 3017526 |
| the association between serological titers in infectious bovine rhinotracheitis virus, bovine virus diarrhea virus, parainfluenza-3 virus, respiratory syncytial virus and treatment for respiratory disease in ontario feedlot calves. | a seroepidemiological study of the association between antibody titers to infectious bovine rhinotracheitis, parainfluenza-3, bovine virus diarrhea and bovine respiratory syncytial viruses, and treatment for bovine respiratory disease was conducted. a total of 322 calves from five different groups were bled on arrival, then one month later all cases (cattle treated for bovine respiratory disease) were rebled together with an equal number of controls (cattle not treated for any disease). titers t ... | 1986 | 3017529 |
| immunological relationships between mumps virus and parainfluenza viruses studied with monoclonal antibodies. | the immunological relationships between mumps virus and parainfluenza viruses were investigated with 74, 78 and 80 previously developed monoclonal antibodies directed against five major structural proteins of mumps virus, sendai virus (a murine parainfluenza type 1 virus) and parainfluenza type 3 virus. these monoclonal antibodies were reacted with the three viruses, with parainfluenza type 2 virus and with newcastle disease virus (ndv) in elisa and immunofluorescence (if) tests. in addition, im ... | 1986 | 3018131 |
| the effect of parainfluenza virus type 3 and pasteurella haemolytica on oxygen-dependent and oxygen-independent bactericidal mechanisms of ovine pulmonary phagocytic cells. | groups of caesarean-derived, colostrum-deprived lambs were inoculated by the intratracheal route with pasteurella haemolytica, either alone or 4 or 6 days after the inoculation of parainfluenza virus type 3 (pi3). other groups were inoculated with pi3 followed by veal infusion broth, or with uninfected cell culture fluid followed by veal infusion broth (controls). all lambs were killed 24 h after the second inoculation. pulmonary phagocytic cells were recovered by lavage and separated into alveo ... | 1986 | 3018994 |
| [use of proteolysis inhibitors for treating parainfluenza infections in an experiment and in the clinic]. | 1986 | 3020805 | |
| seroepidemiological study of para-influenza viruses in preschool children in ghana. | 1986 | 3021968 | |
| molecular cloning and sequence analysis of the human parainfluenza 3 virus mrna encoding the p and c proteins. | the sequence of the mrna encoding the phosphoprotein (p protein) of the human parainfluenza virus 3 (pf3) was determined by molecular cloning. in other parmyxoviridae the p protein mrna is functionally bicistronic and encodes an additional smaller nonstructural protein termed c. in this report three open reading frames (orf) are described. these consist of a single long orf encoding the p protein, and two shorter orfs encoding the structural vp18 protein (analogous to the sendai c protein) and a ... | 1986 | 3022477 |
| synthesis and antiviral evaluation of 1,4-dioxane nucleoside analogues related to nucleoside dialdehydes. | since biologically active nucleoside 2',3'-dialdehydes exist as six-membered cyclic acetals (1) in solution, we have investigated the antiviral activity of some structurally similar 1,4-dioxane nucleoside analogues. by reacting 2',3'-seconucleoside tosylates with base, the guanine (10) and adenine (18) substituted (hydroxymethyl)dioxanes have been constructed. in addition, an unusual adenine-substituted divinyl ether (22) was synthesized via a base-catalyzed, double elimination of a 2',3'-di-o-t ... | 1986 | 3023613 |
| parainfluenza virus type 3 in pigs. | 1986 | 3024379 | |
| differences in bovine parainfluenza 3 virus variants studied by monoclonal antibodies against viral glycoproteins. | we previously showed that of three bovine parainfluenza 3 virus strains the m strain, which is neurovirulent for young mice, has an extensive syncytium-inducing activity, whereas avirulent sc and 910n strains are weak in this activity. it was also demonstrated that both m and sc strains have very low hemagglutination and neuraminidase activities, while the 910n strain shows these activities to high levels. in the present study, monoclonal antibodies (mabs) were raised against the glycoproteins o ... | 1986 | 3024407 |
| reduction in in vivo testing at the national veterinary services laboratories of the united states department of agriculture. | efforts are being made at the national veterinary services laboratories to reduce in vivo testing of usda licensed veterinary vaccines. a hemagglutination test for determining potency of killed parvovirus vaccine is currently being used for canine and swine adjuvanted and nonadjuvanted products; a serum neutralization inhibition test (snit) is being developed for potency testing of killed adjuvanted infectious bovine rhinotracheitis (ibr), bovine virus diarrhea (bvd) and parainfluenza (pi3) vacc ... | 1986 | 3025035 |
| sequence analysis of the p and c protein genes of human parainfluenza virus type 3: patterns of amino acid sequence homology among paramyxovirus proteins. | the complete nucleotide sequence of the p + c mrna of human parainfluenza virus type 3 (pf3) was determined by sequencing cdna, viral genomic rna and mrna. the p + c mrna is 2009 nucleotides in length, exclusive of poly(a), and contains two overlapping open reading frames (orfs). the p + c mrna encodes two proteins, the 602 amino acid nucleocapsid phosphoprotein p and the 199 amino acid non-structural protein c. peptide mapping confirmed that the two proteins are unrelated. hybrid-arrest transla ... | 1986 | 3025346 |
| a bovine respiratory virus vaccination trial. | a respiratory virus vaccination trial was carried out in a commercial calf-rearing unit with a history of virus pneumonia. the effects of vaccination on the incidence of virus respiratory disease and growth rate were assessed. forty-four bought-in calves were allocated to groups and treated as follows: a, unvaccinated controls; b, intranasal temperature-sensitive infectious bovine rhinotracheitis (ibr) vaccine at three and 10 weeks; c, intranasal temperature-sensitive combined ibr and parainflue ... | 1986 | 3026078 |
| [in vitro transcription of human influenza and parainfluenza viruses and its regulation]. | the effect of some factors on in vitro transcription of human influenza and parainfluenza viruses was studied. dinucleotide afg was shown to stimulate transcription of rnp of human parainfluenza type 3 virus and sendai virus in the presence of magnesium but not manganese ions same as in the case of influenza viruses transcription. among two monoclonal antibodies to np protein of influenza virus, clone f 81 inhibited transcription of rnp of human influenza a viruses, and its influence on transcri ... | 1986 | 3026092 |
| [viral etiology of acute respiratory infections in madagascan children]. | a total of 80 nasopharyngeal secretions collected from malagasy children (53 boys and 27 girls) with viral acute respiratory infection, aged from 6 days to 10 year old admitted to the pediatric department of antananarivo general hospital from may to july 1983, were investigated by indirect immunofluorescence method. 54--samples were found positive for respiratory viruses. distribution according age groups and sex has been studied: children belonging to 25-36 month age group and male sex were mor ... | 1986 | 3026268 |
| immunofluorescence in the serological diagnosis of parainfluenza type 3 and respiratory syncytial virus infection in calves. | the fluorescent antibody (fa) test is compared with the haemagglutination inhibition (hi) test for parainfluenza virus type 3 (pi-3) and virus neutralisation (vn) test for respiratory syncytial (rs) virus for detection and titration of virus-specific antibodies. in experimentally inoculated calves pi-3 and rs virus fa tests detected seroconversion at the same time as hi and vn tests, however, in serially diluted sera, the fa test was positive to higher dilution. in studies with paired samples fr ... | 1986 | 3027802 |