Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| intrafetal inoculation of swine with transmissible gastroenteritis virus. | fetuses in 3 sows were inoculated (intramuscularly) with transmissible gastroenteritis (tge) virus on 95th, 77th, and 74th days of the gestation. at 15, 14, and 37 days later (or days when pigs were obtained by hysterectomy), there was evidence of intestinal localization of virus, with villous atrophy and subsequent repair. all intrafetal-inoculated pigs became serologic-positive for tge. a noninoculated pig shown to be seropositive for tge at 15 days of age (after hysterectomy) was resistant to ... | 1978 | 208434 |
| a new coronavirus-like particle associated with diarrhea in swine. | coronavirus-like particles were detected by electron microscopy in the intestinal contents of pigs during a diarrhea outbreak on 4 swine breeding farms. diarrhea was reproduced in experimental pigs with one of the isolates, designated cv777, which was found to be distinct from the 2 known porcine coronaviruses, transmissible gastroenteritis virus and hemagglutinating encephalomyelitis virus. | 1978 | 83132 |
| antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species. | utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ... | 1978 | 81044 |
| demonstration of cytotoxic lymphocytes to virus-infected target cells in pigs inoculated with transmissible gastroenteritis virus. | lymphocytes, cytotoxic to virus-infected target cells, were induced in pigs orally exposed to transmissible gastroenteritis virus. they were studied and experiments were carried out by using autochthonous testicle cells as target cells to avoid genetic incompatibility of effector lymphocytes and target cells. cytotoxic lymphocytes were demonstrated in peyer's patches, mesenteric lymph nodes, spleen, and peripheral blood on postinoculation day (pid) 7. cytotoxic activity of lymphocytes increased ... | 1979 | 223476 |
| humoral and cellular responses in swine exposed to transmissible gastroenteritis virus. | swine exposed to attenuated transmissible gastroenteritis virus had higher virus-neutralizing antibody titers than did swine exposed to virulent virus. the cellular response, measured by the direct leukocyte migration-inhibition (lmi) procedure, was greater in swine exposed to virulent virus than in swine exposed to the attenuated virus. leukocytes from exposed swine were inhibited more in the lmi procedure in the presence of the homologeous sensitizing antigen than in the presence of the hetero ... | 1979 | 222176 |
| passive immunity in transmissible gastroenteritis of swine: immunoglobulin classes of milk antibodies after oral-intranasal inoculation of sows with a live low cell culture-passaged virus. | 1979 | 222177 | |
| stability of porcine colostral immunoglobulins iga, iga2, and igm to proteolytic enzymes. | 1979 | 224734 | |
| concurrent porcine rotaviral and transmissible gastroenteritis viral infections in a three-day-old conventional pig. | a 3-day-old suckling pig with diarrhea was necropsied, and immunofluorescent microscopic examination of the small intestinal mucosa, together with immune electron microscopic examination of the large intestinal contents, provided a presumptive diagnosis of a concurrent infection with transmissible gastroenteritis (tge) virus and porcine rotavirus. immunofluorescent microscopic, immune electron microscopic, and serologic data obtained from gnotobiotic pigs experimentally inoculated with the large ... | 1979 | 224736 |
| lymphocyte proliferative response to viral antigen in pigs infected with transmissible gastroenteritis virus. | development and sequence of lymphocytes reactive to viral antigen in peyer's patches, mesenteric lymph nodes, spleen, and peripheral blood of pigs orally inoculated with transmissible gastroenteritis virus were investigated by a lymphocyte proliferative assay. lymphocytes reactive to the viral antigen were first detected in all the tissues of pigs tested on postinoculation day 7. thereafter, they increased in proliferative reactivity and reached a maximal amount on postinoculation days 10 to 14. ... | 1979 | 217826 |
| a seroepizootiologic study of five viruses in a swine-evaluation station. | this serologic study was done to gain information on the spread, maintenance, and effect upon performance of five porcine viruses. blood samples were taken from two groups of 8- to 11-week-old pigs from a large number of indiana swine herds in a performance-testing station 1 week after entry, 7 weeks after entry (one group only), and at slaughter. the sera were tested by indirect fluorescent antibody tests for antibodies to transmissible gastroenteritis virus (tgev), swine influenza virus (siv), ... | 1979 | 230762 |
| the mucosal lesion in viral enteritis. extent and dynamics of the epithelial response to virus invasion in transmissible gastroenteritis of piglets. | 1979 | 217794 | |
| methanol precipitation of transmissible gastroenteritis virus. | methanol precipitation of transmissible gastroenteritis virus was tested at ph 4.0, 5.0, 6.0, and 7.0 and at methanol concentrations of 15%, 25%, and 30%. supernatant and precipitate fractions were tested for complement-fixing and agar-diffusion soluble antigens and plaque-forming units, and were examined by electron microscopy. virus could be obtained free of detectable agar-diffusion antigens and most of the complement-fixing antigens. most of the virions were without peplomers after methanol ... | 1979 | 43107 |
| virus isolation and serum antibody responses after infection of cats with transmissible gastroenteritis virus. brief report. | transmissible gastroenteritis virus was administered orally to cats. no clinical disease resulted but infectious virus was isolated from faeces for up to 22 days after infection and serum antibody was detected by neutralisation and immunofluorescence tests. | 1979 | 226036 |
| vaccination experiments against transmissible gastroenteritis (tge) of swine. vi. a study of the vaccine virus excretion. | 1979 | 229722 | |
| the neutralization of transmissible gastroenteritis virus by normal heterotypic serum. | exposure of transmissible gastroenteritis virus of pigs to unheated normal heterotypic serum resulted in a drop in infectivity, an effect which was lost after heating the serum to 56 degrees c for 30 min or by treatments inactivating complement. analysis of virus proteins, rna and lipids, and centrifugation studies showed little difference between inactivated and control virus, but electron microscopy of negatively stained particles after treatment with serum revealed holes in the virus envelope ... | 1979 | 217957 |
| [immunity indices in viral (transmissible) gastroenteritis of swine]. | 1979 | 229625 | |
| transmissible gastroenteritis in neonatal dogs: experimental intestinal infection with transmissible gastroenteritis virus. | fourteen neonatal dogs (4 through 11 days of age) were exposed orally to the purdue strain of transmissible gastroenteritis (tge) virus, and six dogs of similar age were noninoculated controls. clinical signs of enteric disease did not develop. both exposed and control dogs had normal fecal passages and appetite throughout the experiment. jejunal epithelium from dogs euthanatized at 12, 24, 48, and 96 hours and at 10 days after exposure did not exhibit morphologic alterations detectable by light ... | 1979 | 229745 |
| [indirect hemagglutination reaction in viral (transmissible) gastroenteritis in swine]. | 1979 | 219585 | |
| experimental and field evaluation of a new oral vaccine for tge. | 1980 | 6256930 | |
| [concentration of viruses and virus-specific antigens by ultrafiltration with membranes developed in the gdr]. | a cellulose acetate membrane, jointly developed by the institute of dairy research in oranienburg and the institute of polymer chemistry in teltow, was successfully used to concentrate by factors between ten and 40 virus suspensions of transmissible gastroenteritis and foot and mouth disease with a protein level of roughly 0.1 per cent in a 400-ml cell with magnetic agitation. the procedure took about two hours, with working pressures being between 0.2 mpa and 0.4 mpa. virus yield in the residue ... | 1980 | 6257195 |
| [isolation and identification of a coronavirus, the causative agent of transmissible gastroenteritis of swine]. | 1980 | 6251347 | |
| direct immunoelectron microscopy of transmissible gastroenteritis virus with immunoglobulins g and a and guinea pig complement. | porcine colostral immunoglobulin (ig)g and iga, isolated from transmissible gastroenteritis virus-infected sows, were compared by direct immunoelectron microscopy. it was estimated, using antibodies with a less than a twofold difference in virus-neutralizing activity, that igg was 500 times more efficient than was iga for coating transmissible gastroenteritis virions. guinea pig complement enhanced the antibody coating with igg, but did not increase virus-neutralizing activity of igg or iga. | 1980 | 6244762 |
| [comparing investigations for detection of antibodies against transmissible gastroenteritis (tge)-virus by use of the microneutralization test, the indirect immunofluorescence test and the virus inhibition test (author's transl)]. | 1980 | 6249564 | |
| characterization of immunoglobulin a antibody in serum of swine inoculated with transmissible gastroenteritis virus. | sera and contents of the small intestine from swine inoculated with transmissible gastroenteritis (tge) virus were examined for immunoglobulin (ig) a antibody responses by the radioimmunoprecipitation test with antiporcine iga serum. when swine were orally inoculated with virulent virus, iga antibody activity was detected in the contents of the small intestine and in serum from postinoculation days 7 to 56. however, iga antibody activity was not detected in the contents of the small intestine or ... | 1980 | 6250429 |
| [use and effectiveness of live vaccines in industrialized livestock production]. | there is an inseparable correlation between higher livestock performance and animal health with many visible repercussions in terms of further intensification of livestock farming. this is the background against which immunobiological action is defined as a major element of all efforts which are undertaken for the purpose of ensuring high-stability and high-continuity and no-problem production. underlined in this paper is the great role played by legal provisions for immunisation concepts as wel ... | 1980 | 6251768 |
| induction of active immunity to tge in neonatal pigs nursing seropositive dams. | 1980 | 6254223 | |
| possible factors influencing the immunoglobulin g concentration in swine colostrum. | the immunoglobulin (ig) g concentration in swine colostrum was determined by the single radial immunodiffusion method, using 157 samples collected from farm-raised sows in the yamaguchi prefecture of japan during 1976 and 1977. the mean igg value was 53.03 mg/ml, and the maximum and minimum values were 101.39 mg/ml and 11.74 mg/ml, respectively. the amount of igg varied greatly among sows. to clarify the possible factors influencing the amount of igg in colostrum, the following items were survey ... | 1980 | 6254410 |
| [trials to develop an immunoprophylaxis for transmissible gastroenteritis (tge) of swine. iv. replication and excretion of the vaccine virus tge b1-300 following vaccination of sows and feeder pigs]. | 1980 | 6255708 | |
| vaccination experiments against transmissible gastroenteritis (tge) of swine. vii. immunoglobulin characteristics of antibodies in milk of sows vaccinated with the "ckp" strain of tge virus. | 1980 | 6263068 | |
| secretory immune response in intestinal mucosa and salivary gland after experimental infection of pigs with transmissible gastroenteritis virus. | pigs 8 to 10 weeks of age were orally infected with transmissible gastroenteritis (tge) virus or infected by inoculation of the virus into thirty-vella loops of jejunum. concentrations of immunoglobulin (ig) a, igm, and igg in serum, saliva, jejunal secretions, loop secretions, and bile were determined by solid-phase radioimmunoassay for tge virus-infected and control pigs. a multiple-staining fluorescent antibody technique was used to determine the relative numbers of iga-, igm-, and igg-produc ... | 1980 | 7192522 |
| detection of antibody against transmissible gastroenteritis virus of pigs by indirect immunoperoxidase antibody test. | immunoperoxidase intibody (ipa) test was developed for detecting antibody against transmissible gastroenteritis (tge) virus of pigs. the ipa antibody titers in sera collected in the field from 82 pigs were approximately seven times higher than those obtained in a serum-neutralization test. the correlation between the tge antibody concentrations in the ipa and serum neutralization tests was positive (r = +0.74). the ipa tests appears to have the potential for routine laboratory use for serologic ... | 1980 | 6244761 |
| mild transmissible gastroenteritis in pigs suckling vaccinated sows. | a strain of transmissible gastroenteritis (tge) virus of low virulence was isolated from 14-day-old pigs suckling sows vaccinated with an attenuated tge vaccine. diarrhea developed in suckling pigs approximately 14 days after farrowing in 4 farrowings; however, none of these pigs died from diarrhea. diarrhea ceased after the 4th farrowing, when vaccination of sows was discontinued. experimentally, both the field isolate and the vaccine strain were infective and in some instances lethal for 2-day ... | 1980 | 6245050 |
| experimental infection of pigs with a new porcine enteric coronavirus, cv 777. | cesarean-derived colostrum-deprived and conventionally reared pigs were orally inoculated with the coronavirus-like agent, cv 777, isolate from an outbreak of epizootic diarrhea in swine of all ages. viral particles detected by electron microscopy in the feces and intestinal contents of inoculated pigs had the typical coronavirus morphology. the present studies provided further evidence that this coronavirus-like agent is different from the two known porcine coronaviruses, transmissible gastroen ... | 1980 | 6245603 |
| genome of porcine transmissible gastroenteritis virus. | the purdue strain of transmissible gastroenteritis virus, a porcine coronavirus, was grown to titers of greater than 10(8) pfu/ml in a swine testicle cell line, and the rna was isotopically labeled with [3h]uridine. the rna was extracted from purified virus and was found to have the following properties. (i) it consisted primarily of a homogeneous large-molecular-weight species which electrophoretically migrated with an apparent molecular weight of 6.8 x 10(6) under denaturing conditions. (ii) i ... | 1980 | 6246272 |
| enzyme linked immunoassay and fluorescent antibody techniques in the diagnosis of viral diseases using staphylococcal protein-a instead of anti-gamma-globulins. | staphylococcal protein-a (spa) is known to interact with the crystallizable fragment (fc) of igg molecules from several species. in the present study, spa coupled to either fluorescein isothiocyanate (fitc) or peroxidase was used in place of antisera to igg for the fluorescent antibody (fa) techniques and the enzyme linked immunoassay (elisa). the spa conjugates produced low background staining when applied in these techniques, and provide a rapid, highly specific and sensitive means for the ide ... | 1980 | 15612263 |
| [transmissible gastroenteritis in a swine-raising unit of 1,200 productive sows. 1. clinical course, disease control and piglet loss]. | 1981 | 6282232 | |
| serum immunoglobulin a antibody response in swine infected with transmissible gastroenteritis virus, as determined by indirect immunoperoxidase antibody test. | using an immunoperoxidase antibody test, the serum immunoglobulin (ig) a antibody titer was determined in swine naturally infected with transmissible gastroenteritis (tge; group a), swine inoculated orally with high-virulent tge virus (group b), and swine inoculated im (group c) or orally (group d) with low-virulent tge virus. studies were then made on the relationship between active immunity to tge and the serum iga antibody titer. in group a swine, serum iga antibody and virus-neutralizing (vn ... | 1981 | 7023294 |
| in vitro properties of low- and high-passaged strains of transmissible gastroenteritis coronavirus of swine. | 1981 | 6267962 | |
| the structure of the canine coronavirus. | 1981 | 6278898 | |
| physicochemical properties of field and cell culture-attenuated strains of swine transmissible gastroenteritis (tge) coronavirus. | 1981 | 6269408 | |
| [transmissible gastroenteritis in a swine raising unit of 1,200 productive sows. 2. results of serological follow-up studies]. | 1981 | 6282233 | |
| high interferon titer in newborn pig intestine during experimentally induced viral enteritis. | we looked for the presence of interferon in the digestive tract of newborn piglets infected with a virulent strain of transmissible gastroenteritis virus (coronaviridae). high levels of type 1 interferon activity were found early in the disease in jejunal and ileal parts of the intestine as well as in the serum. enterocytes appeared to be involved in the interferon synthesis. these findings raise the question of the role of interferon in the pathogenesis of viral enteritis. | 1981 | 6163724 |
| coronavirus cell-associated rna-dependent rna polymerase. | 1981 | 6278878 | |
| an enteric coronavirus infection of cats and its relationship to feline infectious peritonitis. | an enteric coronavirus that is antigenically closely related to feline infectious peritonitis virus (fipv) is ubiquitous in the cat population. this virus has been designated feline enteric coronavirus to differentiate it from fipv. the virus is shed in the feces by many seropositive cats; in catteries it is a cause of inapparent to mildly severe enteritis in kittens 6 to 12 weeks of age. the virus may produce a more severe enteritis in young specific-pathogen-free kittens. feline enteric corona ... | 1981 | 6267960 |
| [inhibiting activity of amantadine (1-aminoadamantane) on the transmissible gastroenteritis virus of swine in cell cultures]. | studies were carried out on the inhibiting activity of amantadine - 1-amino adamantine (1-aa) on the virus of swine transmissive gastroenteritis, rakovski strain, in cell cultures of pig kidney and thyroid gland. the inhibiting agent was tested in concentration of 6.25 to 100 microgram/cm3. parallel titrations with and without 1-aa (25, 50, and 100 microgram/cm3) were used to establish the maximum difference in the titers 2 lg. the effect of inhibition was manifested at minimum concentration of ... | 1981 | 6283726 |
| [transmissible gastroenteritis in a swine raising unit of 1,200 productive sows. 3. course of selection-based eradication]. | 1981 | 6282234 | |
| [morphology of coronaviruses-- electron microscopic demonstration by negative contrast technic of the transmissible gastroenteritis virus of swine]. | this contribution to the morphology of corona virus is based on the use of electron microscopy in a negative contrast investigation of the virus of transmissible gastroenteritis of swine. viruses of transmissible gastro-enteritis are spherical or, in rare cases, pleomorphous particles of typical corona virus structure. the viruses investigated by the authors were 132 nm in diameter. the surface projections, consisting of a flattened knob and conical shaft, are 18 nm in length. internal bodies, 5 ... | 1981 | 6264880 |
| properties of the "ckp" strain of transmissible gastroenteritis virus. ii. studies on the in vivo viral replication in newborn piglets. | 1981 | 6266235 | |
| inner structures of some coronaviruses. | when treated with formaldehyde, tween 80, sodium oleate and nonidet p-40, avian infectious bronchitis virus, porcine transmissible gastroenteritis virus, neonatal calf diarrhea coronavirus, porcine hemagglutinating encephalomyelitis virus as well as the human coronavirus show similar inner structures by negative staining. the first one is an inner membranous bag. this structure could be evaginated following treatments used and does not show the characteristic projections of coronaviruses. subseq ... | 1981 | 6266623 |
| the polypeptide structure of canine coronavirus and its relationship to porcine transmissible gastroenteritis virus. | canine coronavirus (ccv) isolate 1-71 was grown in secondary dog kidney cells and purified by rate zonal centrifugation. polyacrylamide gel electrophoresis revealed four major structural polypeptides with apparent mol. wt. of 203800 (gp204), 49800 (p50), 31800 (gp32) and 21600 (gp22). incorporation of 3h-glucosamine into gp204, gp32 and gp22 indicated that these were glycopolypeptides. comparison of the structural polypeptides of ccv and porcine transmissible gastroenteritis virus (tgev) by co-e ... | 1981 | 6267172 |
| thermal inactivation studies of a coronavirus, transmissible gastroenteritis virus. | the thermolability of transmissible gastroenteritis virus (tgev) was studied between 31 and 55 degrees c using two different strains. the loss of infectivity followed first order kinetics except at the highest temperature. the values of the thermodynamic parameters indicated that the mechanisms involved above and below 45 degrees c are clearly distinct. the rates of inactivation were greater at alkaline than at neutral ph, yet the name of the reaction appeared unchanged. using four independent s ... | 1981 | 6273497 |
| three-dimensional sequential study of the intestinal surface in experimental porcine cv 777 coronavirus enteritis. | 1981 | 6275638 | |
| isolation of transmissible gastroenteritis virus, pseudorabies virus, and porcine enterovirus from pharyngeal swabs taken from market-weight-swine. | pharyngeal swab samples were collected at a central iowa abattoir from 6,010 market-weight slaughter hogs from september 1, 1979 to august 31, 1980. the swab samples were examined for cytopathic viruses by inoculation of monolayer cultures of continuous line of swine testicular cells. of the 6,010 swab samples tested, transmissible gastroenteritis virus was isolated from 91 (1,51%), pseudorabies virus was isolated from 431 (7.17%), and porcine enterovirus was isolated from 21 (0.35%). although a ... | 1981 | 6278996 |
| an immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, cv 777, and several coronaviruses. | a possible antigenic relationship between the porcine enteropathogenic coronavirus-like agent (cvla) and 6 known coronaviruses was examined by immunoelectron microscopy (iem) and by immunofluorescence (if). cvla did not show cross reactivity with infectious bronchitis virus, transmissible gastroenteritis virus (tgev), canine coronavirus (ccv) hemagglutinating encephalomyelitis virus (hev), neonatal calf diarrhea coronavirus (ncdcv) or feline infectious peritonitis virus (fipv). antigenic relatio ... | 1981 | 6166280 |
| laboratory evaluation of selected disinfectants as virucidal agents against porcine parvovirus, pseudorabies virus, and transmissible gastroenteritis virus. | of a variety of disinfectants evaluated, only sodium hypochlorite and sodium hydroxide inactivated porcine parvovirus (ppv) after a 5-minute incubation period. after the same incubation time, pseudorabies and transmissible gastroenteritis viruses were inactivated by all of the disinfectants tested. when the incubation time was increased to 20 minutes, 2% glutaraldehyde and a double-strength concentration of a commercial formaldehyde preparation also inactivated ppv. formaldehyde vapor and ultrav ... | 1981 | 6269467 |
| lesions in the small intestine of newborn pigs inoculated with porcine, feline, and canine coronaviruses. | the infectivity and pathogenicity to newborn pigs of antigenically related coronaviruses from pigs (transmissible gastroenteritis virus; tgev), cats (feline infectious peritonitis virus; fipv), and dogs (canine gastroenteritis virus; cgev) were studied by light, scanning electron, and immunofluorescence microscopy. hysterectomy-derived, 12-hour-old pigs were orally given tissue culture or frozen preparations of 6 coronavirus strains (3 porcine, 2 feline, and 1 canine). the pigs were killed at re ... | 1981 | 6168221 |
| antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses. | transmissible gastroenteritis virus of swine (tgev), feline infectious peritonitis virus (fipv), and canine coronavirus were studied with respect to their serological cross-reactivity in homologous and heterologous virus neutralization, immune precipitation of radiolabeled tgev, electroblotting, and enzyme-linked immunosorbent assay using individual virion polypeptides prepared by polyacrylamide gel electrophoresis. tgev was neutralized by feline anti-fipv serum, and the reaction was potentiated ... | 1982 | 6182101 |
| rna-dependent rna polymerase activity in coronavirus- infected cells. | an enzymatic activity which incorporates [3h]ump into acid-precipitable material in the presence of endogenous template was found in the cytoplasm of porcine cells infected with the transmissible gastroenteritis virus of swine. this activity was not found in uninfected control cells, nor was it found in purified virus. the activity was associated with the mitochondrial fraction of infected cells, suggesting that the enzyme is membrane bound. the activity required the presence of all three ribonu ... | 1982 | 6283135 |
| intestinal, pulmonary, and serum antibody responses of feeder pigs exposed to transmissible gastroenteritis virus by the oral and the oral-intranasal routes in inoculation. | specific antibodies against transmissible gastroenteritis (tge) virus produced as a result of oral and oral-intranasal viral exposure of feeder pigs were isolated by molecular sieve column chromatography of lung wash, intestinal fluid, and serum samples. selected fractions were analyzed for neutralizing antibody activity. oral inoculation gave a predominantly immunoglobulin (ig) g antibody response in lung wash and serum, as compared with the response in intestinal fluid in which neutralizing an ... | 1982 | 6283957 |
| [detection of antibodies against the virus causing viral gastroenteritis in swine using the modified autoradiography test]. | a modified method for autoradiographic determination of antibodies to the virus of transmissible gastroenteritis of swine was developed. it is based on the actual reaction between antigen bound in cells of the infected cell cultures and antibodies in tested sera, which is visualized, by help of rabbit antibodies marked 125j (125j rasw igg antibody) to porcine igg, on a sensitive radiograph and evaluated on the basis of darkening at the point of positive immunological reaction. specificity of the ... | 1982 | 6291217 |
| [effect of coronavirus infection on the delayed hypersensitivity reaction]. | 1982 | 6292669 | |
| serum antibody responses of neonatal and young adult pigs to transmissible gastroenteritis coronavirus. | serum titers of virus-neutralizing (vn) antibody were 10 to 16 times higher in neonatal pigs than in young adult pigs, after single oral doses of virulent transmissible gastroenteritis virus (tgev). to determine the reason for this higher response, sera from neonatal and young adult pigs, 18 to 21 days after exposure to tgev, were collected and assayed for vn antibody by plaque reduction. in addition, sera of vn-positive and vn-negative neonatal pigs were analyzed for immunoglobulin classes by r ... | 1982 | 6293157 |
| applications of peroxidase-antiperoxidase staining technique for detection of transmissible gastroenteritis virus in pigs. | the peroxidase-antiperoxidase (pap) staining technique was used for the detection of transmissible gastroenteritis virus (tgev) in small intestines of tgev-infected 8-week-old pigs and in infected mcclurkin pig testicle cells by means of light microscopy. the specific-positive reaction was characterized by the presence of many brown granules of various sizes in the cytoplasm of infected cells. nonspecific granules caused by endogenous peroxidases in the cytoplasm of eosinophils stained by pap we ... | 1982 | 6283953 |
| [effect of amantadine (1-aminoadamantan) on different phases of the replicative cycle of transmissible gastroenteritis virus of swine]. | 1982 | 6287708 | |
| studies of enteric coronaviruses in a feline cell line. | development is reported of a feline cell line which can support the growth of coronaviruses from canine (ccv), feline (fipv) and porcine (tgev) species. the cell culture has been serially transferred over 100 times and has retained its initial growth requirements, proliferative capacity and morphologic features. each virus had specific growth characteristics in this cell culture although all produced a similar cpe and plaques under agar. cross neutralization studies demonstrated a two-way relati ... | 1982 | 6298992 |
| [determination of the quantity of minimal antigen for oral immunization of sows against transmissible gastroenteritis of swine using riems tge vaccine]. | 1982 | 6291484 | |
| changes in gut-associated lymphoid tissues of the small intestine of eight-week-old pigs infected with transmissible gastroenteritis virus. | the responses of the interepithelial lymphocytes (iel) and aggregated lymph nodules (aln; peyer's patches) of the small intestines of 8-week-old pigs to transmissible gastroenteritis virus (tgev) infection were characterized at 12, 18, and 24 hours after pigs were inoculated. there was no significant difference in numbers of iel between control and tgev-infected pigs at 12 and 18 hours. however, in pigs examined at 24 hours, there was a significant decrease in the number of iel in the duodenum a ... | 1982 | 7091819 |
| porcine neonatal coccidiosis in quebec. | intestinal coccidiosis was diagnosed in 110 diarrheic piglets originating from 66 farrowing operations. these piglets had been submitted alive for necropsy early after the beginning of diarrhea, and the diagnosis of coccidiosis was based on the demonstration of coccidial forms in their jejunum by histopathological examination. enteropathogenic strains of escherichia coli were demonstrated in only nine of the 110 piglets while the transmissible gastroenteritis virus and rotavirus were each presen ... | 1982 | 17422160 |
| passive immunity to transmissible gastroenteritis virus: intramammary viral inoculation of sows. | sows were injected intramammarily with live-attenuated tge virus, an enteric coronavirus--one sow during pregnancy and three sows during lactation. all sows were tge antibody seronegative prior to inoculation except for one naturally infected sow inoculated during lactation. the animal injected during pregnancy had primarily igg tge antibodies in milk from all glands. by contrast, sows injected during lactation had iga and igm initially, and later iga and igg tge antibodies in milk from injected ... | 1983 | 6575719 |
| macrotiter assay for coronavirus-neutralizing activity in cats using a canine continuous cell line (a-72). | a heterologous neutralization assay for feline infectious peritonitis virus serology was developed using a single continuous cell line of canine origin, a-72, which is susceptible to cytopathic infection with both transmissible gastroenteritis virus of pigs and canine coronavirus. of several coronavirus isolates tested, the 1-71 isolate of canine coronavirus demonstrated the most effective neutralization by serum and body fluids of cats with histopathologically confirmed feline infectious perito ... | 1983 | 6319813 |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| [comparative studies using the immunofluorescence technic in experimental transmissible gastroenteritis and rotavirus infection of piglets]. | 1983 | 6303246 | |
| interferon induction in rotavirus and coronavirus infections: a review of recent results. | the interferon (ifn) response was studied in two different models of viral enteritis of the neonate. in rotavirus infected calves, ifn synthesis could be detected in the intestine and in the blood at the time of the diarrheic symptoms. a kinetic study on canulated calves suggested that systemic ifn is of intestinal origin. during tgev infection (transmissible gastroenteritis virus) of the newborn piglet, an acute disease which leads to 100% mortality, ifn was found at very high titres (1 000-20 ... | 1983 | 6203463 |
| influence of infectivity of transmissible gastroenteritis virus of swine by pancreatin and some unknown factors. | the infectivity of virulent strain, not attenuated strain, of transmissible gastroenteritis virus (tgev) of swine could be enhanced as much as 50-fold by pancreatin incorporated medium. the mechanism(s) of enhancement was uncertained. multiplication of tgev, either virulent or attenuated strain, was inhibited by intestinal fluid of both tgev infected and noninfected piglets. pelleting virus particles from intestine fluid was likely to remove all inhibitors and resulted in facilitating the isolat ... | 1983 | 6094119 |
| antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against cells infected with porcine transmissible gastroenteritis virus. | the objective of this study was to determine whether porcine peripheral blood leukocytes and intestinal intraepithelial leukocytes can mediate antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis virus. peripheral blood leukocytes collected from six young adult pigs and intraepithelial leukocytes from a further five pigs were used as effector cells in chromium release assays against pk-15 cells p ... | 1983 | 6315197 |
| identification of porcine transmissible gastroenteritis virus in house flies (musca domestica linneaus). | transmissible gastroenteritis (tge) virus was detected in house flies (musca domestica linneaus) by staining with specific fluorescent antibody. the flies were collected within a swine confinement facility in which tge was enzootic. laboratory-reared flies were infected experimentally with tge virus and the virus was recovered from the insects for 72 hours after infection. the tge virus was identified both by the fluorescent antibody technique and by isolation in cell culture. the nature of plaq ... | 1983 | 6316821 |
| a viral subunit immunogen for porcine transmissible gastroenteritis. | an immunogenic component was isolated from both the illinois (propagated in young swine) and the miller (cell culture-adapted) strains of porcine transmissible gastroenteritis (tge) virus. the viral subunit was released from the virion by sonication and was separated from intact virus and other viral components by isopycnic centrifugation. it had a buoyant density in sucrose of 1.02 g/ml. further purification consisted of gel filtration through sephadex g200, in which process the immunogen, with ... | 1983 | 6316850 |
| lactogenic immunity to transmissible gastroenteritis virus induced by a subunit immunogen. | a subunit prepared from transmissible gastroenteritis (tge) virus and used to immunize 24 gilts prior to farrowing induced production of specific antibody in the serum and milk. challenge of pigs, two to seven days of age and suckling on the vaccinated gilts, with the illinois strain of tge virus resulted in morbidity of 28% and mortality of 4% as compared with 100 and 73%, respectively, for control piglets. piglets nursing on a sow which had been immunized approximately 10 months previously wer ... | 1983 | 6099641 |
| [the activity of chemical preparations with respect to the transmissible gastroenteritis virus and enterovirus of swine]. | 1983 | 6101074 | |
| [interferonogenic activity of coronavirus in in vitro and in vivo experiments]. | 1983 | 6086047 | |
| enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera. | an enzyme-linked immunosorbent assay (elisa) was developed for detection and quantification of serum antibodies to transmissible gastroenteritis virus (tgev) in swine. sera from pigs inoculated with cell culture-origin tgev or gut-origin tgev were tested for anti-tgev antibody by elisa and by serum virus-neutralization test (nt). the elisa detected antibody 3 days (av) sooner than did the nt when sera from pigs inoculated with cell culture-origin tgev were tested and 1 day sooner than did the nt ... | 1984 | 6089625 |
| [transmissible gastroenteritis of swine: stability of coronavirus in gastric and intestinal contents]. | low and high passaged cell culture strains of tge coronavirus were examined for stability in gastric and small intestine juices collected in 3-6 month-old pigs killed at different times after last feeding. results revealed high fragility of the tge virus in these digestive liquids. differences in stability were observed between strains of tge virus. but no correlation could be made between the level of stability and cell passage status of the virus strain. we conclude that the stability of tge c ... | 1984 | 6097154 |
| [cultivation of the transmissible gastroenteritis virus in a continuous cell line]. | cell cultures of the permanent cell line spev to which the transmissive gastroenteritis virus had already been adapted were used to culture the virus and carry out the virus-neutralization test. use was made of a cell suspension of a variable density--300 and 500 thou cells per cm3. both variants of the cell suspension were comparatively studied in terms of growth, the production of a monolayer, susceptibility to infection, and titer of the virus obtained, using 4 test tubes with the virus at va ... | 1984 | 6093344 |
| efficacy of vaccination of sows with serologically related coronaviruses for control of transmissible gastroenteritis in nursing pigs. | three groups of pregnant sows were vaccinated at 8 and 2 weeks before parturition with tissue culture-adapted feline infectious peritonitis (fip) virus, porcine transmissible gastroenteritis (tge) small-plaque (sp) virus from a persistently infected cell line, or noninfected cell culture fluids (controls). pigs nursing vaccinated sows were orally challenge exposed with virulent tge virus when they were 1 to 3 days old. the morbidity of the nursing pigs was 48% in the sp-tge group, 82% in the fip ... | 1984 | 6093647 |
| [transmissible gastroenteritis: vaccination of sows in contaminated environment using inactivated virus in oil emulsion]. | an oil emulsion vaccine against tge was prepared using a virus suspension produced in tissue culture and inactivated by formaline. intramuscular vaccination trials were performed on sows in the field from two herds (167 sows) which had been infected by tge virus more than one year previously. considering qualities of immunity after natural infection, the objective of the work was to try to give a new impetus to immunity in such herds using vaccination as a booster. after two vaccine injections t ... | 1984 | 6097153 |
| the effect of adoptive transfer of mononuclear leukocytes from an adult donor on spontaneous cell-mediated cytotoxicity and resistance to transmissible gastroenteritis in neonatal piglets. | the purpose of this study was to attempt to establish spontaneous cell-mediated cytotoxicity effector activity in the intraepithelial lymphocytes of neonatal piglets by adoptive transfer of mononuclear leukocytes from an adult donor and to determine the effect of transfer on the resistance of piglets to transmissible gastroenteritis. cytotoxicity was determined by a chromium release assay using pk-15 cells persistently infected with transmissible gastroenteritis virus as targets. the experimenta ... | 1984 | 6239678 |
| experimental inoculation of cats with canine coronavirus and subsequent challenge with feline infectious peritonitis virus. | oronasal inoculation of minimal-disease cats with a virulent field isolate of canine coronavirus elicited a neutralizing antibody response that increased steadily over a two-month period, indicating in vivo amplification of the inoculated dose. a heterologous neutralizing antibody response to transmissible gastroenteritis virus could be detected three weeks after titers to canine coronavirus were first observed and was found to parallel the homologous response at a level approximately one log10 ... | 1984 | 6097763 |
| replication of transmissible gastroenteritis coronavirus (tgev) in swine alveolar macrophages. | several strains of the enteropathogenic coronavirus transmissible gastroenteritis virus (tgev) have been shown to replicate in alveolar macrophages maintained in vitro. a distinct cytopathic effect was observed at a multiplicity of infection greater than or equal to 0.1. infected cells released infectious virus. the extent of both virus production and cell destruction was highly dependent upon the virus input. at low input, cell viability was affected only slightly, and a delayed and persistent ... | 1984 | 6319578 |
| significance of lysosomes in the morphogenesis of coronaviruses. | virus-containing electron-dense membrane-bound cytoplasmic bodies are described in tracheal epithelial cells of chickens infected with infectious bronchitis virus and in intestinal epithelial cells of swine infected with porcine epidemic diarrhea virus. using silver-methenamine staining, phosphotungstic acid staining and acid phosphatase enzyme cytochemical staining of ultra-thin sections, these bodies were shown to be virus-containing secondary lysosomes and residual bodies. the accumulation of ... | 1984 | 6320768 |
| defective replication of porcine transmissible gastroenteritis virus in a continuous cell line. | during a search for established cell lines to produce large quantities of porcine transmissible gastroenteritis virus (tgev), we observed bright immunofluorescent staining 6- 12h after infection of pig kidney derived llc-pk1 line. infectious virus yield was, however, 2 log10 lower than that from secondary adult pig thyroid (apt/2) cell cultures, although small plaques were visible by three days in cultures maintained under agarose, suggesting limited replication. attempts to adapt tgev to the ll ... | 1984 | 6331129 |
| colonic compensation in transmissible gastroenteritis of swine. | absorption of water and electrolytes by the small and large intestine was examined using a nonabsorbable marker technique in 3-day-old and 3-wk-old pigs. one-half of the pigs in each group were orally infected with transmissible gastroenteritis virus; the remaining pigs served as controls. three-day-old control pigs concentrated the nonabsorbable fluid marker twelve fold along the small and large intestine, indicating an efficiency of about 95% in absorption of the exogenous daily fluid load pre ... | 1984 | 6714576 |
| influence of coronavirus (transmissible gastroenteritis) infection on jejunal myoelectrical activity of the neonatal pig. | four silver-silver chloride electrodes were surgically implanted at 5-cm intervals on the jejunal serosa of 7 neonatal pigs. daily recordings, 7 h in duration, were made from each piglet beginning 3 days after surgery. characteristic migrating motility complexes and short, distinct (2.5-5.0 s), rapidly aboral migrating bursts of intense spike activity ("migrating action potential complexes") were seen in all preinfection recordings. piglets were inoculated with a 1-ml oral dose of a 0.1% gut sus ... | 1984 | 6735081 |
| antibody-dependent and spontaneous cell-mediated cytotoxicity against transmissible gastroenteritis virus infected cells by lymphocytes from sows, fetuses and neonatal piglets. | the purpose of this study was to investigate the ontogeny in the pig of effector lymphocytes mediating antibody-dependent and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis virus. the same activities were also studied in sows in late pregnancy and early lactation. peripheral blood lymphocytes and intraepithelial lymphocytes collected from piglets during the first week of life failed to mediate cytolysis as determined by chromium release as ... | 1984 | 6332661 |
| hypoglycemia: a factor associated with low survival rate of neonatal piglets infected with transmissible gastroenteritis virus. | the main purpose of this work was to study changes in the balance of fluids, electrolytes and blood metabolites in neonatal piglets with severe transmissible gastroenteritis. six two day old conventional piglets were infected with transmissible gastroenteritis virus while six others were used as normal controls. blood samples were collected in heparin when the infected piglets were moribund. the following variables were measured: packed red cell volume, total plasma protein and bicarbonate, bloo ... | 1984 | 6478297 |
| altered jejunal permeability to macromolecules during viral enteritis in the piglet. | we studied the macromolecular permeability of segments of jejunum from 2-wk-old piglets after the animals had been experimentally infected with an invasive enteric virus, transmissible gastroenteritis virus. jejunal segments were mounted in ussing chambers at stages of the infection, and permeability was measured using three probe molecules of differing molecular weights. in control tissue, permeability to horseradish peroxidase was 2.6 times higher across segments with peyer's patches than acro ... | 1985 | 3918915 |
| fluid therapy trials in neonatal piglets infected with transmissible gastroenteritis virus. | the main purpose of this study was to evaluate the effectiveness of an oral fluid therapy alone or combined with parenteral administration of a 5% dextrose solution to attenuate the clinical signs and the pathophysiological consequences of transmissible gastroenteritis in neonatal piglets. eighteen two day old conventional piglets were infected with transmissible gastroenteritis virus while six others were used as controls (group 1). at the onset of diarrhea, infected piglets were divided into t ... | 1985 | 4075236 |
| intestinal phospholipase b activity in pigs inoculated with transmissible gastroenteritis virus. | intestinal phospholipase b activity in pigs inoculated with transmissible gastroenteritis (tge) virus was studied. phospholipase activity was quantified by measuring the hydrolytic release of free fatty acids in homogenized intestinal tissue incubated with lysophosphatidylcholine. an increase in enzyme activity was observed in the cranial and caudal portions of the ileum and jejunum in pigs killed 3, 6, and 8 days after inoculation with tge virus. seemingly, phospholipase b may be part of the ho ... | 1985 | 4026032 |
| plaque assay and neutralization test for porcine transmissible gastroenteritis virus in cultures of swine cell lines. | 1985 | 3022059 | |
| studies of tgev spike protein gp195 expressed in e. coli and by a tge-vaccinia virus recombinant. | the gene coding for the surface spike protein gp195 of tgev has been cloned and expressed in e. coli in the form of fusion proteins. these proteins were isolated and used to immunize laboratory animals. all animals developed antibodies cross-reacting with the tgev virion but failed to neutralize the virus. the entire gp195 gene was also inserted into vaccinia to generate a tgev-vaccinia recombinant virus (vtge) that expressed tgev gp195. animals vaccinated with vtge produced neutralizing antibod ... | 1985 | 3000150 |
| [diagnosis of transmissible gastroenteritis in swine by the agar gel immunodiffusion test]. | a transmissive gastroenteritis antigen was obtained from intestinal content of pigs with symptoms of the disease, the presence of which was demonstrated by the agar gel immunodiffusion test. it was shown that the antigen was rough and lacking in purity, but it proved to be sensitive and specific. in the conditions of agar gel immunodiffusion in the presence of a reconvalescent serum the antigen produced a clearly expressed precipitation line for 24 to 48 hours. a correlation was also found with ... | 1985 | 3002011 |