Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| tgev corona virus orf4 encodes a membrane protein that is incorporated into virions. | the coding potential of the open reading frame orf4 (82 amino acids) of transmissible gastroenteritis virus (tgev) has been confirmed by expression using a baculovirus vector. five monoclonal antibodies (mabs) raised against the 10k recombinant product immunoprecipitated a polypeptide of a similar size in tgev-infected cells. immunofluorescence assays performed both on insect and mammalian cells revealed that orf4 was a membrane-associated protein, a finding consistent with the prediction of a m ... | 1992 | 1316677 |
| seroconversion of pigs in contact with dogs exposed to canine coronavirus. | in order to determine if canine coronavirus (ccv) could be transmitted to pigs, two dogs were inoculated orally with virulent ccv. after 24 h, the dogs were moved to an isolation room that contained three three-day-old pigs. a wire mesh fence, allowing close contact between the animals, separated the dogs from the pigs. the dogs and pigs were observed for 14 days for clinical signs of disease. samples of blood were obtained from dogs and pigs immediately before the dogs were inoculated with viru ... | 1992 | 1316800 |
| efficacy of antiserum produced in goats and pigs to passively protect piglets against virulent transmissible gastroenteritis virus. | the protective effect of sera produced in swine and goats exposed to virulent transmissible gastroenteritis virus (tgev) or modified-live tgev was tested in hysterectomy-derived, colostrum-deprived three-day-old pigs. pigs were given serum with their daily ration of milk, and their immunity to virulent tgev was determined. the pigs were observed for ten days for clinical signs of tgev infection. one of nine pigs receiving goat serum was protected whereas all three pigs receiving three doses of s ... | 1992 | 1317247 |
| transmissible gastroenteritis virus antibody production in vitro by porcine peripheral blood leukocytes. | the purpose of this study was to demonstrate the in vitro production of transmissible gastroenteritis virus (tgev)-specific antibodies by peripheral blood leukocytes (pbl) harvested from piglets infected with tgev. piglets were infected with the virulent purdue strain of tgev and at intervals postinfection their pbl were cultivated in the presence of tgev antigen, control antigen or pokeweed mitogen (pwm). the culture supernatants were tested for tgev antibodies by a fixed cell enzyme immunoassa ... | 1992 | 1317248 |
| coronavirus infection in mink (mustela vison). serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus. | antibodies to a transmissible gastroenteritis virus (tgev)-related coronavirus have been demonstrated in mink sera by indirect immunofluorescence, peroxidase-linked antibody assays and immunoblotting. this is the first serological evidence of a specific coronavirus infection in mink. the putative mink coronavirus (mcv) seems to be widespread in the danish mink population with a prevalence approaching 100%. analysis by immunoblotting has shown that mcv is closely related to tgev by the spike (s), ... | 1992 | 1319622 |
| genetic evolution and tropism of transmissible gastroenteritis coronaviruses. | transmissible gastroenteritis virus (tgev) is an enteropathogenic coronavirus isolated for the first time in 1946. nonenteropathogenic porcine respiratory coronaviruses (prcvs) have been derived from tgev. the genetic relationship among six european prcvs and five coronaviruses of the tgev antigenic cluster has been determined based on their rna sequences. the s protein of six prcvs have an identical deletion of 224 amino acids starting at position 21. the deleted area includes the antigenic sit ... | 1992 | 1326823 |
| effect of fixation on the detection of transmissible gastroenteritis coronavirus antigens by the fixed-cell immunoperoxidase technique. | the effect of various fixatives and detergents on the in vitro detection of the viral determinants which are expressed in swine testis cells infected with the transmissible gastroenteritis coronavirus (tgev) was studied using a microwell immunoperoxidase technique. when compared with glutaraldehyde and formaldehyde, 0.1% paraformaldehyde was found to be the fixative of choice for the detection of these determinants on the membranes of infected cells. among dehydrating fixatives, 80% acetone or a ... | 1992 | 1328393 |
| transmissible gastroenteritis coronavirus: surface antigens induced by virulent and attenuated strains. | three strains of the transmissible gastroenteritis virus (tgev) possessing different degrees of pathogenicity for piglets were examined for their capacity to express m and s glycoproteins on the infected cell surface using a microwell immunoperoxidase test. these two viral glycoproteins were easily detected on the plasma membrane of 0.1% paraformaldehyde-fixed swine testis (st) or pig kidney (rp.d) cells which were infected with high-passaged purdue-115 and low-passaged d-52 strains and a high-p ... | 1992 | 1329166 |
| genomic organization and expression of the 3' end of the canine and feline enteric coronaviruses. | the genomic organization at the 3' end of canine coronavirus (ccv) and feline enteric coronavirus (fecv) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (fipv) and transmissible gastroenteritis virus (tgev) of swine. comparison of the latter two has previously revealed an extra open reading frame (orf) at the 3' end of the fipv genome, lacking in tgev, which is currently designated orf 6b. both ccv and fecv possess 6b-related orfs at the 3' ends of ... | 1992 | 1329312 |
| antigenic variant of swine influenza virus causing proliferative and necrotizing pneumonia in pigs. | a new antigenic variant of swine influenza virus was isolated from the lungs of pigs experiencing respiratory problems in 7 different swine herds in quebec. pigs of different ages were affected, and the main clinical signs were fever, dyspnea, and abdominal respiration. coughing was not a constant finding of the syndrome. at necropsy, macroscopic lesions included the overall appearance of pale animals, general lymphadenopathy, hepatic congestion, and consolidation of the lungs. histopathologic f ... | 1992 | 1333815 |
| interferon induction in porcine leukocytes with transmissible gastroenteritis virus. | leukocytes were harvested from the peripheral blood, mesenteric lymph node and small intestinal lamina propria from groups of three piglets before, and 1, 2 and 3 weeks after infection with virulent transmissible gastroenteritis virus (tgev) at 2 weeks of age. the donor piglets developed clinical signs of transmissible gastroenteritis which persisted for up to 3 days, and they developed peak serum titres of tgev-neutralizing antibodies 2 weeks post-infection. the leukocytes were cultured in the ... | 1992 | 1345591 |
| aminopeptidase n is a major receptor for the entero-pathogenic coronavirus tgev. | coronaviruses, like many animal viruses, are characterized by a restricted host range and tissue tropism. transmissible gastroenteritis virus (tgev), a major pathogen causing a fatal diarrhoea in newborn pig, replicates selectively in the differentiated enterocytes covering the villi of the small intestine. to investigate the molecular determinants of the infection, we characterized the surface molecule used by the virus for binding and entry into host cells. here we report that aminopeptidase n ... | 1992 | 1350661 |
| antigenic structure of transmissible gastroenteritis virus nucleoprotein. | a group of 11 monoclonal antibodies (mabs) raised against transmissible gastroenteritis virus (tgev) was used to study the antigenic structure of the virus nucleoprotein (n). to identify the regions recognized by mabs, dna fragments derived from the n-coding region of the tgev strain fs772/70 were cloned into pur expression plasmids and the antigenicity of the resulting fusion proteins was analyzed by immunoblotting. a major antigenic domain was identified, covering the first 241 amino acid resi ... | 1992 | 1373552 |
| antigenic variation among transmissible gastroenteritis virus (tgev) and porcine respiratory coronavirus strains detected with monoclonal antibodies to the s protein of tgev. | five nonneutralizing monoclonal antibodies (mab) generated to the virulent miller strain of transmissible gastroenteritis virus (tgev) and specific for the s protein were characterized. competition assays between purified and biotinylated mab indicated that mab 75b10 and 8g11 mapped near a new subsite, designated v and 2 mab, 44c11 and 45a8, mapped to a previously designated subsite d. a fifth mab mapped between subsites v and e. these mab were tested with 3 previously characterized mab to subsi ... | 1992 | 1379786 |
| isolation of sequences from a random-sequence expression library that mimic viral epitopes. | we describe the use of random peptide sequences for the mapping of antigenic determinants. an oligonucleotide with a completely degenerate sequence of 17 or 23 nucleotides was inserted into a bacterial expression vector. this resulted in an expression library producing random hexa- or octapeptides attached to a beta-galactosidase hybrid protein. mimotopes, or antigenic sequences that mimic an epitope, were selected by immunoscreening of colonies with monoclonal antibodies, which were specific fo ... | 1992 | 1380046 |
| epitope specificity of protective lactogenic immunity against swine transmissible gastroenteritis virus. | the epitope specificity of the protective immune response against swine transmissible gastroenteritis (tge) has been investigated by using circulating and secretory antibodies. this study was carried out with sows vaccinated with tgev or the antigenically related porcine respiratory coronavirus (prcv). tgev vaccination of sows resulted in greater lactogenic protection of suckling piglets against tgev challenge and a higher secretory immune response than prcv vaccination did. these differences in ... | 1992 | 1383566 |
| monoclonal antibody analysis of neutralization and antibody-dependent enhancement of feline infectious peritonitis virus. | fifty-four monoclonal antibodies (mabs) to feline infectious peritonitis virus (fipv) were characterized according to protein specificity, immunoglobulin subclass, virus neutralization, reactivity with different coronaviruses, and ability to induce antibody-dependent enhancement (ade) of fipv infection in vitro. the mabs were found to be specific for one of three structural proteins of fipv. a total of 47 mabs were specific for the 205-kda spike protein (s), 3 mabs were specific for the 45-kda n ... | 1992 | 1383568 |
| effect of concentration and source of sulfate on nursery pig performance and health. | the effect of sulfate in drinking water at concentrations of 600, 1,200, and 1,800 mg/l on nursery pig performance and health was evaluated over 28 days on 415 weaned pigs. sodium sulfate and magnesium sulfate were evaluated in combination at concentrations of 600, 1,200, and 1,800 mg/l, and independently at concentrations of 600 and 1,800 mg/l in the drinking water. seven treatment groups and 1 control group were evaluated for mean gain, feed consumption, water consumption, feed conversion, pre ... | 1992 | 1429159 |
| analysis of a 9.6 kb sequence from the 3' end of canine coronavirus genomic rna. | we have analysed the organization of the 3' end of the genomic rna of canine coronavirus (ccv), a virus which has a close antigenic relationship to transmissible gastroenteritis virus (tgev), porcine respiratory coronavirus (prcv) and feline infectious peritonitis virus (fipv). genomic rna isolated from ccv strain insavc-1-infected a72 cells was used to generate a cdna library. overlapping clones, spanning approximately 9.6 kb [from the 3' end of the polymerase gene, 1b, to the poly(a) tail] wer ... | 1992 | 1431811 |
| an elisa for detection of antibodies against porcine epidemic diarrhoea virus (pedv) based on the specific solubility of the viral surface glycoprotein. | viral proteins of porcine epidemic diarrhoea virus (pedv) were extracted from the cytoplasm of infected vero cells using hypotonic conditions and a non-ionic detergent. both the ph and the nacl concentration of the extraction buffer were varied in attempts to increase the solubility of the virion spike glycoproteins (s-protein) and of the nucleocapsid proteins (n-protein). monoclonal antibodies, hyperimmune sera and convalescent pig sera were used to identify and monitor these proteins by immuno ... | 1992 | 1441196 |
| radioactive and enzymatic cloned cdna probes for bovine enteric coronavirus detection by molecular hybridization. | genomic rna of f15 strain bovine enteric coronavirus (becv) was cloned in e. coli. three clones (174, 160, pg78), selected in the cdna library, including a large portion of the nucleocapsid (n), matrix (m) and peplomeric (s) protein genes, were used as probes for a slot blot hybridization assay. two probe labelling techniques were compared, radiolabelling with 32p and enzymatic labelling through covalent linkage to peroxidase and chemiluminescence detection. the radioactive probe 174 detected as ... | 1992 | 1642553 |
| differentiation between transmissible gastroenteritis virus and porcine respiratory coronavirus using a cdna probe. | a plasmid, pg3bs, containing a cdna clone from the 5' coding region of the peplomer glycoprotein gene appears to be specific for enteric transmissible gastroenteritis virus (tgev) strains and for live-attenuated tgev vaccines. this cdna probe is used to differentiate porcine respiratory coronavirus (prcv) isolates from tgev field and vaccine strains by a slot blot hybridization assay. probe pg3bs also hybridizes to canine coronavirus (ccv) rna but does not hybridize to antigenically related feli ... | 1991 | 1645595 |
| expression and cellular localisation of porcine transmissible gastroenteritis virus n and m proteins by recombinant vaccinia viruses. | porcine transmissible gastroenteritis virus (tgev) nucleoprotein and integral membrane protein genes were cloned into the vaccinia virus insertion vector, pgs20, in the correct orientation for expression under the control of the vaccinia p7.5k promoter. recombinant vaccinia viruses were generated by in vivo homologous recombination of the insertion vector with the wr strain of vaccinia virus. nucleoprotein (n) expressed by both recombinant vaccinia virus and tgev had a relative molecular mass (m ... | 1991 | 1645905 |
| localization of major neutralizing epitopes on the s1 polypeptide of the murine coronavirus peplomer glycoprotein. | a recombinant baculovirus system has been used to express the amino terminal half of the murine coronavirus (jhmv) peplomer glycoprotein in insect cells. the expressed polypeptide is glycosylated and is recognized by a set of monoclonal antibodies (mabs) specific for jhmv s protein. three of these mabs have a very high neutralizing activity for jhmv but not for other mhv strains. these results indicate that jhmv-specific, major neutralizing epitopes reside in the amino terminal s1 subunit of the ... | 1991 | 1645909 |
| inhibition of virus attachment to cpk cells by monoclonal antibody to transmissible gastroenteritis virus. | five mabs, which recognized e2 glycoprotein of tge virus to-163 and showed neutralizing activity, were examined to see if they inhibit virus attachment to the susceptible cells (cpk cells). only one (160/4) mab blocked the virus attachment to the cells, indicating that the inhibition of virus attachment is one of important mechanisms of neutralizing by antibody. | 1991 | 1649515 |
| serological studies of transmissible gastroenteritis in great britain, using a competitive elisa. | a competitive elisa which differentiates between transmissible gastroenteritis virus (tgev) and porcine respiratory coronavirus (prcv) was used to detect non-neutralising antibodies to the peplomer protein of tgev in porcine sera. the test was shown to be tgev specific, having a relative specificity of 100 per cent, and to have a relative sensitivity of 94.9 per cent when compared with the virus neutralisation test. the prevalence of tgev in great britain is low; only 0.6 per cent of sows sample ... | 1991 | 1651028 |
| prevalence of pseudorabies virus infection and associated infections in six large swine herds in illinois. | sera were collected from 6 large farrow-to-finish swine herds infected with pseudorabies virus (prv) in illinois. all herds were participating in the large herd cleanup study, a usda-initiated project to evaluate the feasibility of eradicating pseudorabies from large farms (greater than 400 sows) by use of a combination of vaccination and management changes. herd size ranged between 425 and 1,500 breeding females. between april and july 1990, sera for measurement of prv antibodies were obtained ... | 1991 | 1651912 |
| antiviral activity against transmissible gastroenteritis virus, and cytotoxicity, of natural porcine interferons alpha and beta. | porcine interferon (poifn)-alpha prepared in primed peripheral blood leukocyte cultures induced with newcastle disease virus and poifn-beta from pk-15 cell cultures induced with polyinosinic:polycytidylic acid were partially purified by precipitation with potassium thiocyanate and anion exchange chromatography. mean purification factors in terms of units of poifn per mg of protein, of 37 and 12 were obtained for poifn-alpha and poifn-beta respectively. in yield reduction assays in swine testis a ... | 1991 | 1653103 |
| antigenic analysis of feline coronaviruses with monoclonal antibodies (mabs): preparation of mabs which discriminate between fipv strain 79-1146 and fecv strain 79-1683. | we prepared 31 monoclonal antibodies (mabs) against either fipv strain 79-1146 or fecv strain 79-1683, and tested them for reactivity with various coronaviruses by indirect fluorescent antibody assay (ifa). sixteen mabs which reacted with all of the 11 strains of feline coronaviruses, also reacted with canine coronavirus (ccv) and transmissible gastroenteritis virus (tgev). in many of them, the polypeptide specificity was the recognition of transmembrane (e1) protein of the virus. we succeeded i ... | 1991 | 1653482 |
| a capture-enzyme immunoassay for rapid diagnosis of transmissible gastroenteritis virus. | two enzyme immunoassays (eia) were developed for the detection of swine transmissible gastroenteritis virus (tgev) antigens. the 2 eias used the same detecting system, a monoclonal antibody conjugated to horseradish peroxidase, but used different capture systems including a monoclonal antibody (m-eia) or a polyclonal antibody (p-eia). the eias were compared with the fluorescent antibody test (fat) and electron microscopy (em) for the detection of tgev in intestinal samples of experimentally inoc ... | 1991 | 1654131 |
| nucleocapsid or spike protein-specific cd4+ t lymphocytes protect against coronavirus-induced encephalomyelitis in the absence of cd8+ t cells. | to investigate the antiviral cd4+ t cell response in coronavirus mhv-jhm-induced encephalomyelitis, spleen and thymic lymphocytes from diseased rats were stimulated in culture with virus ag, expanded and tested for their specificity to viral proteins and nucleocapsid (n) and spike (s) proteins that had been expressed in bacteria. a strong t cell response specific for n was measurable during acute disease, whereas s-specific t cells were only detectable in rats with a later onset of disease. cd4+ ... | 1991 | 1655890 |
| processing and antigenicity of entire and anchor-free spike glycoprotein s of coronavirus tgev expressed by recombinant baculovirus. | the s gene of transmissible gastroenteritis virus (tgev) was inserted into the genome of autographa californica nuclear polyhedrosis virus (acnpv) using the transfer plasmid pvl941. infection of sf9 insect cells with the recombinant virus resulted in the synthesis of a 175k polypeptide which was able to trimerize and was transported to the cell surface as is the authentic tgev s protein. despite the lack of complete carbohydrate processing, the recombinant s protein exhibited antigenic propertie ... | 1991 | 1660201 |
| proteins specified by swine transmissible gastroenteritis virus: identification of non-structural proteins by two-dimensional electrophoresis. | four virus-induced non-structural proteins with apparent molecular weights of 11-14 kilodaltons (kda) were identified by two-dimensional electrophoresis in cells infected by tgev. differences in the number of non-structural proteins were observed among virulent and attenuated tgev strains as well as with two antigenically related feline and canine coronaviruses. | 1991 | 1662051 |
| outbreaks in quebec pig farms of respiratory and reproductive problems associated with encephalomyocarditis virus. | encephalomyocarditis virus (emcv) was isolated from tissues of aborted fetuses and weaned and suckling piglets from 4 different pig farms in quebec. the farms were experiencing reproductive failure in sows of different parities concomitant to respiratory problems in suckling and postweaning piglets. at necropsy, gross lesions were confined to the lung and consisted of pulmonary congestion and edema of various degrees. lesions of multifocal interstitial to proliferative pneumonia were found in th ... | 1991 | 1662074 |
| enzyme immunofiltration assay for measurement of antibodies to transmissible gastroenteritis virus of swine: comparison with enzyme immunosorbence assay, serum neutralization, and indirect immunofluorescent antibody techniques. | 1991 | 1662083 | |
| the cloning and sequencing of the virion protein genes from a british isolate of porcine respiratory coronavirus: comparison with transmissible gastroenteritis virus genes. | previous analysis of porcine respiratory coronavirus (prcv) mrna species showed that mrnas 2 and 3 were smaller than the corresponding transmissible gastroenteritis virus (tgev) mrna species (page et al. (1991) j. gen. virol. 72, 579-587). sequence analysis showed that mrna 3 was smaller due to the presence of a new putative rna-leader binding site upstream of the prcv orf-3 gene. however, this observation did not explain the deletion observed in prcv mrna 2. polymerase chain reaction (pcr) was ... | 1991 | 1662846 |
| fixed-cell immunoperoxidase technique for the study of surface antigens induced by the coronavirus of transmissible gastroenteritis (tgev). | an immunoperoxidase technique performed on the tgev-infected cells was developed for detection of virus-induced antigens. the presence of m antigen of tgev on the surface of infected cells was demonstrated by this technique. this finding is in contrast to the m protein of murine hepatitis coronavirus which migrates to the golgi apparatus but is not transported to the plasma membrane. the time course of appearance m and s antigens on the surface of tgev-infected cell can be studied by this techni ... | 1991 | 1663290 |
| histocompatible miniature pig (d/d haplotype): generation of hybridomas secreting a or m monoclonal antibody. | two hypoxanthine/aminopterin/thymidine-sensitive cell sublines (l142 and l231) have been derived from independent lymphoblastoid cell lines of b lineage. after propagation for more than 100 population doublings (1 year) in culture, these cells still retained a doubling time between 19 to 20 hours, near diploïdy and relatively low (l142) and high (l231) secretion rate of m immunoglobulins. near diploid hybrid cells were easily generated with leukocytes from the spleen, the gut lamina propria or t ... | 1991 | 1667778 |
| characterization and reactivity of monoclonal antibodies to the miller strain of transmissible gastroenteritis virus of swine. | hybridomas secreting monoclonal (mab) to transmissible gastroenteritis virus (tgev) were produced by fusion of sp2/0 myeloma cells and splenic lymphocytes of balb/c mice immunized with the virulent cell-passaged miller strain of tgev. the mab secreted by these hybridomas were partially characterized; 4 of them (ma4, ma5, mh11, mb2) had high-neutralization titer for tgev. the remaining 7 (mc6, md9, me5, mg5, mf2, me9, mg7) did not neutralize tgev at 1:25 dilution. all 4 neutralizing and 2 of the ... | 1990 | 1689128 |
| antigenic homology among coronaviruses related to transmissible gastroenteritis virus. | the antigenic homology of 26 coronavirus isolates, of which 22 were antigenically related to transmissible gastroenteritis virus (tgev), was determined with 42 monoclonal antibodies. type, group, and interspecies specific epitopes were defined. two group specific mabs distinguished the enteric tgev isolates from the respiratory variants. an antigenic subsite involved in neutralization was conserved in porcine, feline, and canine coronavirus. the classification of the human coronavirus 229e in a ... | 1990 | 1689525 |
| the effect of interferon induction in newborn piglets on the humoral immune response to oral vaccination with transmissible gastroenteritis virus. | groups of newborn piglets were vaccinated orally with a modified live transmissible gastroenteritis (tge) virus vaccine at 3 days and 13 days of age, and treated with the synthetic interferon (ifn) inducer polyinosinic:polycytidylic acid (poly iclc) at 2, 3 or 4 days of age. control groups consisted of piglets which were vaccinated but not treated with poly iclc, as well as piglets which were treated with poly iclc but not vaccinated. significantly higher mean ifn titres were produced in respons ... | 1990 | 1692649 |
| four major antigenic sites of the coronavirus transmissible gastroenteritis virus are located on the amino-terminal half of spike glycoprotein s. | four major antigenic sites have been delineated on the spike protein (s) of the porcine enteric coronavirus transmissible gastroenteritis virus (tgev) in previous topological studies using monoclonal antibodies (mabs). correlation of these sites with the physical structure of the protein was achieved by use of different approaches. recombinant pex plasmids directing the synthesis of various fused s polypeptides were constructed. a hybrid protein containing nine s-specific residues (363 to 371) w ... | 1990 | 1693663 |
| analysis and simulation of a neutralizing epitope of transmissible gastroenteritis virus. | the amino acid sequences recognized by monoclonal antibodies (mabs) specific for the antigenic site iv of the spike protein s of transmissible gastroenteritis virus were analyzed by pepscan. all mabs of group iv recognized peptides from the s region consisting of residues 378 to 390. in addition, the neutralizing mabs (subgroup iv-a) also bound to peptides from the region consisting of residues 1173 to 1184 and to several other peptides with a related amino acid composition. the contribution of ... | 1990 | 1693702 |
| expression of swine transmissible gastroenteritis virus envelope antigens on the surface of infected cells: epitopes externally exposed. | the peplomer protein (s) and the transmembrane protein (m) of transmissible gastroenteritis virus (tgev) of swine were identified by iodination and serologically on the surface of infected cells. of a total of 4 monoclonal antibodies (mab) directed against four antigenic sites of s protein (correa et al., 1988), 3 specific for sites a, b and d attached to the plasma membrane of infected cells, as disclosed by indirect immunofluorescence and by complement-mediated cytolysis. four of the mabs assa ... | 1990 | 1697441 |
| characterization of monoclonal antibodies against feline infectious peritonitis virus type ii and antigenic relationship between feline, porcine, and canine coronaviruses. | seven monoclonal antibodies (mabs) with neutralizing activity against feline infectious peritonitis virus (fipv) strain 79-1149 (type ii) were prepared. when the polypeptide specificity recognized by these monoclonal antibodies (mabs) was investigated by western immunoblotting, all of the mabs reacted with peplomer glycoprotein (s) of the virus. by competitive binding assay these mabs were found to recognize at least 3 different epitopes. the reactivity of these mabs with 6 viruses classified as ... | 1991 | 1706593 |
| immunogenicity of peptides simulating a neutralization epitope of transmissible gastroenteritis virus. | previously, an epitope recognized by a set of neutralizing monoclonal antibodies directed against the s protein of transmissible gastroenteritis has been identified. this neutralization epitope can be simulated by a single peptide combining residues 380 to 387 and 1176 to 1184 of the s protein; this combination peptide (sffsygei-qlakdkvne) was more antigenic than it single constituents. here we describe the immunogenicity of this combination peptide, in comparison with monomer and tandem peptide ... | 1991 | 1708930 |
| residues involved in the antigenic sites of transmissible gastroenteritis coronavirus s glycoprotein. | the s glycoprotein of transmissible gastroenteritis virus (tgev) has been shown to contain four major antigenic sites (a, b, c, and d). site a is the main inducer of neutralizing antibodies and has been previously subdivided into the three subsites aa, ab, and ac. the residues that contribute to these sites were localized by sequence analysis of 21 mutants that escaped neutralization or binding by tgev-specific monoclonal antibodies (mabs), and by epitope scanning (pepscan). site a contains the ... | 1991 | 1711257 |
| background paper. mapping epitopes on coronavirus glycoproteins. | 1990 | 1715655 | |
| enteric coronavirus tgev: mapping of four major antigenic determinants in the amino half of peplomer protein e 2. | 1990 | 1715657 | |
| location of antigenic sites of the s-glycoprotein of transmissible gastroenteritis virus and their conservation in coronaviruses. | 1990 | 1715658 | |
| linear neutralizing epitopes on the peplomer protein of coronaviruses. | 1990 | 1715660 | |
| an elisa for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus. | a competition elisa utilizing a mab directed towards a peplomer protein epitope common to tgev, prcv and related feline and canine coronaviruses is described. | 1991 | 1717102 |
| a conserved coronavirus epitope, critical in virus neutralization, mimicked by internal-image monoclonal anti-idiotypic antibodies. | monoclonal antibody (mab) 6a.c3 neutralizes transmissible gastroenteritis coronavirus (tgev) and is specific for a conserved epitope within subsite ac of the spike (s) glycoprotein of tgev. six hybridomas secreting anti-idiotypic (ab2) mabs specific for mab 6a.c3 (ab1) have been selected. all six mabs inhibited the binding of ab1 to tgev and specifically cross-linked mab1-6a.c3. four of these hybridomas secreted gamma-type anti-idiotypic mabs. the other two ab2s (mabs 9a.g3 and 9c.e11) were reco ... | 1991 | 1719237 |
| intestinal permeability in piglets during transmissible gastroenteritis. | macromolecular permeability of the small intestine was tested in seven three-week-old piglets infected with porcine transmissible gastroenteritis virus (tge-strain miller). fourteen hours after the infection, the piglets showed loss of appetite and a profuse diarrhoea. in some animals vomiting occurred somewhat earlier. macromolecular permeability was tested morphologically by injecting horseradish peroxidase (mw = 40,000 da) into the jejunal lumen just distally to the treitz' ligament in two pi ... | 1991 | 1830439 |
| differentiation of transmissible gastroenteritis virus from porcine respiratory coronavirus and other antigenically related coronaviruses by using cdna probes specific for the 5' region of the s glycoprotein gene. | two cdna clones prepared from the virulent miller strain of transmissible gastroenteritis virus (tgev) were identified, and their nucleotide sequences were determined. the clones were nonoverlapping and located in the 5' region of the s glycoprotein gene. their nucleotide and predicted amino acid sequences were compared with published sequences of the attenuated purdue strain of tgev and feline infectious peritonitis virus (fipv). tgev clone pe21 contained 381 bp of the s glycoprotein gene and h ... | 1991 | 1847152 |
| primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens. | feline infectious peritonitis virus (fipv) causes a mostly fatal, immunologically mediated disease in cats. previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the fipv spike protein (s) induced early death after challenge with fipv (vennema et al., 1990, j. virol. 64, 1407-1409). in this paper we describe similar immunizations with the fipv membrane (m) and nucleocapsid (n) proteins. the genes encoding these proteins were cloned and sequenced. comparison o ... | 1991 | 1847259 |
| detection of transmissible gastroenteritis virus using cdna probes. | five cdna probes prepared from molecular clones representing genomic rna sequences of the virulent miller strain of transmissible gastroenteritis virus (tgev) were used in a dot blot hybridization assay to detect tgev in cell culture and fecal specimens. two clones (pa2 and pb4) represent nucleotide base pairs at the 3' terminus of the miller tgev genome. the other three clones represent various portions of the 5' end of the e2 gene, which codes for the major surface glycoprotein of tgev. each o ... | 1991 | 1848070 |
| alteration of the ph dependence of coronavirus-induced cell fusion: effect of mutations in the spike glycoprotein. | infection of susceptible murine cells with the coronavirus mouse hepatitis virus type 4 (mhv4) results in extensive cell-cell fusion at phs from 5.5 to 8.5. the endosomotropic weak bases chloroquine and ammonium chloride do not prevent mhv4 infection. in marked contrast, we have selected variants from a neural cell line persistently infected with mhv4 which are entirely dependent on acid ph to fuse host cells and are strongly inhibited by endosomotropic weak bases. wild-type and variant viruses ... | 1991 | 1848311 |
| sequence comparison of the 5' end of mrna 3 from transmissible gastroenteritis virus and porcine respiratory coronavirus. | analysis of porcine transmissible gastroenteritis virus (tgev) and porcine respiratory coronavirus (prcv) mrna species indicated a deletion in mrna 3 of prcv. polymerase chain reaction (pcr) was used to clone the 5' end of mrna 3 from prcv for comparison with the equivalent region in tgev. small deletions were observed within and around the prcv sequence equivalent to the putative open reading frame (orf) orf-3a identified in tgev. the potential rna polymerase-leader complex binding site (leader ... | 1991 | 1848593 |
| glycosylation is required for coronavirus tgev to induce an efficient production of ifn alpha by blood mononuclear cells. | porcine peripheral blood mononuclear cells (pbmc) are induced to produce interferon alpha (ifn alpha) following in vitro exposure to coronavirus tgev (transmissible gastroenteritis virus)-infected glutaraldehyde-fixed cell monolayers or to tgev virions. in the present report, we examined the possibility that glycosylation of viral proteins could play a major role in interactions with pbmc leading to the production of ifn alpha. con a pretreatment of tgev-infected cell monolayers before fixation ... | 1991 | 1850168 |
| infection of pigs with transmissible gastroenteritis virus from contaminated carcases. | sixteen 6-month-old pigs were exposed to transmissible gastroenteritis (tge) virus by placing them in close contact with piglets infected at 1 week of age. fourteen of the older pigs were slaughtered between 1 and 5 d after exposure to infection and their carcases dressed in simulated abattoir conditions. samples of muscle, bone marrow and carcase lymph nodes were stored at -25 degrees c for at least 30 d and then homogenised and fed to groups of 1-week-old and 3-week-old pigs. four of 12 one-we ... | 1991 | 1850235 |
| induction of lactogenic immunity to transmissible gastroenteritis virus of swine using an attenuated coronavirus mutant able to survive in the physicochemical environment of the digestive tract. | a transmissible gastroenteritis (tge) coronavirus mutant (188-sg), selected as attenuated and resistant to acidity and proteases of the digestive tract of adult pigs, was used as vaccine ("nouzilly strain") in sows to protect suckling piglets against a challenge exposure carried out with a highly virulent tgev strain. the pregnant sows were immunized once (42-49 days before farrowing) or twice (42-49 and 7-15 days before farrowing) by the oral, intramuscular or conjunctival route with the 188-sg ... | 1991 | 1850894 |
| intracellular processing of the porcine coronavirus transmissible gastroenteritis virus spike protein expressed by recombinant vaccinia virus. | the spike (s) protein from a virulent british field isolate of porcine transmissible gastroenteritis virus (tgev) fs772/70 was constructed from cdna and inserted into the vaccinia virus (vv) thymidine kinase gene locus under the control of the vv early/late gene p7.5k promoter. recombinant s protein was synthesized as an endo-beta-n-acetylglucosaminidase h (endo h)-sensitive glycoprotein with high mannose simple oligosaccharides (gp 190) that underwent post-translational modification to an endo ... | 1991 | 1850927 |
| localization of extensive deletions in the structural genes of two neurotropic variants of murine coronavirus jhm. | the intracellular rna of two neurotropic variants of the jhm strain of mouse hepatitis virus (mhv) independently isolated from the brain and spinal cord of an infected wistar furth rat were compared with that of the parental virus. the mrnas corresponding to the genes encoding the peplomer (s) and the hemagglutinin-esterase (he) proteins of the variant viruses were found to be smaller in size. the possible sequence changes were studied by oligonucleotide fingerprinting and direct rna sequencing. ... | 1991 | 1850936 |
| oral transmission of transmissible gastroenteritis virus by muscle and lymph node from slaughtered pigs. | a study was conducted in the usa to determine whether transmissible gastroenteritis (tge) virus could be transmitted from carcases of slaughtered pigs. transmissible gastroenteritis virus was transmitted to 6-day-old piglets by dosing with homogenates of muscle and lymph node collected from 500 clinically normal pigs at the time of slaughter. all piglets in 2 separately housed litters showed clinical signs of tge with 5 piglets dying within 10 d of oral dosing with homogenates. transmissible gas ... | 1991 | 1850981 |
| genetic analysis of porcine respiratory coronavirus, an attenuated variant of transmissible gastroenteritis virus. | the genome and transcriptional pattern of a newly identified respiratory variant of transmissible gastroenteritis virus were analyzed and compared with those of classical enterotropic transmissible gastroenteritis virus. the transcriptional patterns of the two viruses indicated that differences occurred in rnas 1 and 2(s) and that rna 3 was absent in the porcine respiratory coronavirus (prcv) variant. the smaller rna 2(s) of prcv was due to a 681-nucleotide (nt) deletion after base 62 of the prc ... | 1991 | 1851885 |
| sequence analysis of the turkey enteric coronavirus nucleocapsid and membrane protein genes: a close genomic relationship with bovine coronavirus. | the 3' end of the turkey coronavirus (tcv) genome and the gene encoding the nucleocapsid protein (n) were cloned and sequenced. the gene encoding the membrane protein (m) was obtained by cloning a polymerase chain reaction (pcr)-amplified fragment obtained using bovine coronavirus (bcv)-specific primers. furthermore, five tcv dna fragments, obtained by pcr on rna from clinical specimens and corresponding to either the n terminus of the m protein or the complete m protein were also cloned and seq ... | 1991 | 1856695 |
| electrolyte transport in piglets infected with transmissible gastroenteritis virus. stimulation by verapamil and clonidine. | the effects of clonidine, an alpha 2-adrenergic agonist, and verapamil, a ca2+ channel blocker, on na+ and cl- absorption were studied in stripped jejunal mucosa from control and transmissible-gastroenteritis-virus-infected piglets. all infected piglets developed severe diarrhea 18-24 hours after oral inoculation. jejunum from infected animals, as compared with control jejunum, had decreased mucosal-to-serosal, serosal-to-mucosal, and net na+ and cl- fluxes. clonidine and verapamil caused a decr ... | 1991 | 1889713 |
| coronavirus motif. | 1991 | 1896083 | |
| experimental induction of diarrhoea in newly-weaned piglets. | to induce diarrhoea and hypovolaemia, newly-weaned conventionally bred piglets (3- to 4-weeks-old), were either given secretagogues or were inoculated with enterotoxigenic escherichia coli (etec). choleratoxin (n = 2), e. coli heat-labile enterotoxin (n = 2) or castor oil (n = 2) were given intragastrically or piglets were intraperitoneally injected with dl 5-hydroxytryptophan (n = 3). these substances induced a transient diarrhoea without clinical symptoms of dehydration. therefore, a combinati ... | 1991 | 1950231 |
| sequence analysis of the leader rna of two porcine coronaviruses: transmissible gastroenteritis virus and porcine respiratory coronavirus. | the leader rna sequence was determined for two pig coronaviruses, transmissible gastroenteritis virus (tgev), and porcine respiratory coronavirus (prcv). primer extension, of a synthetic oligonucleotide complementary to the 5' end of the nucleoprotein gene of tgev was used to produce a single-stranded dna copy of the leader rna from the nucleoprotein mrna species from tgev and prcv, the sequences of which were determined by maxam and gilbert cleavage. northern blot analysis, using a synthetic ol ... | 1990 | 1962975 |
| sequence of the s gene from a virulent british field isolate of transmissible gastroenteritis virus. | subgenomic mrna from a virulent field isolate of porcine transmissible gastroenteritis virus (tgev), strain fs772/70, was used to produce cdna. the cdna from three overlapping clones was sequenced by the chain termination method and two open reading frames (orfs) were identified. the largest orf, 4350bp, encoded a polypeptide of 1449 amino acids of relative molecular mass (mr) 159811, which contained 33 potential n-linked glycosylation sites, a cysteine-rich region, and a potential transmembrane ... | 1990 | 1964522 |
| evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the united states. | a respiratory variant of transmissible gastroenteritis virus (tgev), designated prcv-ind/89, was isolated from a swine breeding stock herd in indiana. the virus was readily isolated from nasal swabs of pigs of different ages and induced cytopathology on primary porcine kidney cells and and on a swine testicular (st) cell line. an 8-week-old pig infected oral/nasally with the respiratory variant and a contact pig showed no signs of respiratory or enteric disease. these pigs did not shed virus in ... | 1990 | 1965638 |
| expression of the porcine transmissible gastroenteritis coronavirus m protein. | cloned cdna encoding the m protein of the porcine transmissible gastroenteritis coronavirus (tgev) was introduced into a vaccinia virus to examine the function of the amino-terminal signal peptide. the m protein expressed by the recombinant virus was targeted to the golgi region of infected cells, as is the m protein in cells infected with tgev. the protein appeared not to undergo processing other than glycosylation. however, the vaccinia-expressed m protein was slightly larger than the protein ... | 1990 | 1966402 |
| expression of the spike protein of murine coronavirus jhm using a baculovirus vector. | the spike (s) protein of murine coronavirus jhm strain (jhmv) has been expressed in insect cells using a recombinant baculovirus vector. the expressed s protein was shown to be glycosylated and expressed on the cell surface, and to be similar in size and antigenic properties to the s protein produced in mouse cells infected by jhmv. however, no proteolytic cleavage was detected in insect cells. the sera from rats immunised with s protein derived from insect cells reacted in immunoprecipitation a ... | 1990 | 1966405 |
| expression of tgev structural genes in virus vectors. | 1990 | 1966407 | |
| nucleotide sequence of the e2-peplomer protein gene and partial nucleotide sequence of the upstream polymerase gene of transmissible gas gastroenteritis virus (miller strain). | the e2-peplomer protein gene of the virulent miller strain of transmissible gastroenteritis virus (tgev) was sequenced from cdna clones and compared to the e2 gene sequence of the avirulent purdue strain. sequence comparisons indicate that most amino acid differences occur in the n-terminal half of the e2-peplomer which represents the most exposed region of the protein. in addition, analysis of an incompletely sequenced open reading frame (orf) to the immediate 5' side of the e2 gene indicates e ... | 1990 | 1966416 |
| coronavirus subgenomic replicons as a mechanism for mrna amplification. | 1990 | 1966420 | |
| genomic organisation of a virulent isolate of porcine transmissible gastroenteritis virus. | 1990 | 1966423 | |
| background paper. the appearance of the porcine respiratory coronavirus has created new problems and perspectives. | 1990 | 1966432 | |
| induction of milk iga antibodies by porcine respiratory coronavirus infection. | an elisa was developed to examine the prevalence of tgev-specific immunoglobulin (ig)a in the milk of sows, infected in the field with prcv or with tgev. it was shown that previous prcv-infections can induce the secretion of iga antibodies in the milk. however, only 9 out of 28 prcv-infected sows had iga in their milk whereas 11 tgev-infected sows all secreted iga. on farms where a reinfection with prcv occurred, the number of iga-secreting sows increased from 2 to 11 on a total of 13 sows. this ... | 1990 | 1966433 |
| sites of replication of a porcine respiratory coronavirus in 5-week-old pigs with or without maternal antibodies. | on farms, where the porcine respiratory coronavirus (prcv) is enzootic, pigs usually become infected between 5 and 10 weeks of age while losing their maternal antibodies. it was examined whether prcv replicates in the small intestine in such pigs. this point is important since intestinal replication with prcv might induce immunity against tgev not only by stimulating mucosal intestinal immunity, but also by the induction of a lactogenic iga response at later age via the gut-mammary link. five we ... | 1990 | 1966434 |
| infection with a new porcine respiratory coronavirus in denmark: serologic differentiation from transmissible gastroenteritis virus using monoclonal antibodies. | in 1984 neutralizing antibodies against transmissible gastroenteritis virus (tgev) were detected in pig herds in a small geographical area in the southern part of denmark. no clinical symptoms were observed and accumulating epidemiological evidence gradually pointed towards a respiratory infection. in 1986 a tge-like virus, tentatively named porcine respiratory coronavirus (prcv), was isolated from the lungs of swine. the virus was partially characterized using monoclonal antibodies against tgev ... | 1990 | 1966435 |
| molecular aspects of the relationship of transmissible gastroenteritis virus (tgev) with porcine respiratory coronavirus (prcv). | 1990 | 1966436 | |
| the s2 subunit of the spike glycoprotein of bovine coronavirus mediates membrane fusion in insect cells. | the hemagglutinin/esterase (he), spike precursor (s) and the s1 and s2 subunits of the spike precursor protein of bovine coronavirus were expressed in spodoptera frugiperda (sf9) cells, and the cell-fusing activity of each recombinant glycoprotein was examined. extensive syncytia formation was observed in cells infected with the s2 recombinant but not with the he or s1 recombinant baculoviruses. fusion of sf9 cells expressing the intact s protein precursor was evident after trypsin treatment. th ... | 1991 | 1984658 |
| minus-strand copies of replicating coronavirus mrnas contain antileaders. | the 5' leader sequence on mrnas of the porcine transmissible gastroenteritis coronavirus was determined and found to be 90 nucleotides in length. an oligodeoxynucleotide with a sequence from within the leader was used as a probe in northern analysis on rna from infected cells, and an antileader (a minus-strand copy of the leader sequence) was shown to be present on all mrna minus-strand species. rnase protection analysis showed the antileader to be approximately the same length as the leader. th ... | 1991 | 1985203 |
| comparison of the nucleotide and deduced amino acid sequences of the s genes specified by virulent and avirulent strains of bovine coronaviruses. | the entire nucleotide sequences of the spike glycoprotein (s) genes of the highly virulent bovine coronavirus (bcv) strain bcv-ly138, the avirulent bcv-l9 and related norden vaccine (bcv-vaccine) strains were determined using the polymerase chain reaction (pcr) to amplify cdnas obtained by reverse transcription of viral rna, and to produce single strand cdnas for dna sequencing. the s gene sequences of these viral strains were compared with those of recently published strains bcv-mebus, bcv-queb ... | 1991 | 2053289 |
| comparison of bovine coronavirus (bcv) antigens: monoclonal antibodies to the spike glycoprotein distinguish between vaccine and wild-type strains. | monoclonal antibodies (mabs) against two major structural proteins of the cell-adapted mebus strain of bovine coronavirus (bcv-l9) were produced and characterized. seven mabs reacted with the peplomeric glycoprotein, gp 100/s, while three mabs reacted with the nucleoprotein p53/n in western blot analysis of bcv polypeptides. mabs to gp 100/s reacted with discontinuous epitopes of gp 100/s in westerns under mild but not under standard denaturing conditions. in contrast, mabs to p53/n reacted in b ... | 1991 | 2053295 |
| structural analysis of the conformational domains involved in neutralization of bovine coronavirus using deletion mutants of the spike glycoprotein s1 subunit expressed by recombinant baculoviruses. | two conformation-dependent neutralizing epitopes, a and b, have been mapped to the s1 subunit of the s spike glycoprotein of bovine coronavirus (bcv). in order to characterize the structure of these antigenic sites, we constructed a series of cdna clones encoding deleted or truncated s1 derivatives and expressed the modified genes in insect cells using recombinant baculoviruses. monoclonal antibodies directed against epitopes a and b recognized only the mutant s1 polypeptides containing amino ac ... | 1991 | 2053298 |
| functional analysis of the coronavirus mhv-jhm surface glycoproteins in vaccinia virus recombinants. | 1990 | 2103098 | |
| biosynthesis and function of the coronavirus spike protein. | 1990 | 2103106 | |
| [the use of genetic engineering in veterinary medicine with examples from epidemiology, diagnosis and drug production]. | the results of genetic engineering have reached practical veterinary medicine already. nevertheless there is a great lack of knowledge among those veterinarians who usually do not work with these methods. therefore we want to give an introduction into the advantages and dangers of this technology concerning veterinary medicine. some important analytical methods are explained. related viruses such as wee and eee or canine parvovirus, feline parvovirus and mink enteritis virus, or the related coro ... | 1990 | 2112273 |
| coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in rna synthesis. | both genomic and subgenomic replicative intermediates (ris) and replicative-form (rf) structures were found in 17cl1 mouse cells that had been infected with the a59 strain of mouse hepatitis virus (mhv), a prototypic coronavirus. seven species of rnase-resistant rf rnas, whose sizes were consistent with the fact that each was derived from an ri that was engaged in the synthesis of one of the seven mhv positive-strand rnas, were produced by treatment with rnase a. because the radiolabeling of the ... | 1990 | 2154591 |
| localization of antigenic sites of the e2 glycoprotein of transmissible gastroenteritis coronavirus. | four antigenic sites of the e2 glycoprotein of transmissible gastroenteritis virus were defined by competitive radioimmunoassays of monoclonal antibodies (mabs). here, we describe the localization of these sites by testing the antigenicity of protein fragments and prokaryotic expression products of e2 gene fragments, and by sequencing of mab-resistant (mar) mutants. partial proteolysis of purified e2 protein allowed the isolation of a 28k fragment recognized by both site a- and site c-specific m ... | 1990 | 2155284 |
| lactogenic immunity to transmissible gastroenteritis (tge) of swine induced by the attenuated nouzilly strain of tge virus: passive protection of piglets and detection of serum and milk antibody classes by elisa. | piglets of eight sows vaccinated by different routes with the attenuated tge mutant coronavirus, nouzilly (n) strain, and piglets from two field seropositive sows were challenged with a virulent tge strain. on the day of challenge and 10 days after challenge, milk and serum samples from sows were analysed for their level of neutralizing antibodies, total immunoglobulin classes and tge antibody classes by an elisa. no direct relationship was seen between the level of protection of the litters and ... | 1990 | 2156374 |
| sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus. | a porcine respiratory coronavirus (prcv) was inoculated by aerosol into nine hysterectomy-derived and colostrum-deprived pigs at the age of one week. they were killed at different times after inoculation and tissues were sampled for virus isolation and immunofluorescence. results indicate that virus replicated to high titres in the respiratory tract. replication mainly occurred in alveolar cells but also in epithelial cells of nasal mucosa, trachea, bronchi, bronchioli, in alveolar macrophages a ... | 1990 | 2159175 |
| the multiplication of transmissible gastroenteritis viruses in several cell lines originated from porcine kidney and effects of trypsin on the growth of the viruses. | plaque formation, replication and related cytopathic function of 9 strains of transmissible gastroenteritis (tge) virus were examined in primary cells and cell lines such as cpk, ib-rs-2, esk, and pk-15 originated from porcine kidney and the effects of trypsin on the replication of tge virus were examined in cpk cells. all strains produced a cytopathic effect and grew well in cpk cells as well as in primary porcine kidney cells. the effect of trypsin on the plaque formation was different from st ... | 1990 | 2161476 |
| mechanisms of transmissible gastroenteritis coronavirus neutralization. | transmissible gastroenteritis virus (tgev) was neutralized more than 10(9)-fold with antibodies of a single specificity [monoclonal antibodies (mabs)]. most of the virus was neutralized in the first 2-3 min of a reversible reaction, which was followed by a second phase with a decreased neutralization rate and, in some cases, by a persistent fraction, which was a function of the mab and of the antibody-to-virus ratio. neutralization of tgev is a specific event that requires the location of the ep ... | 1990 | 2164725 |
| serologic survey for transmissible gastroenteritis virus neutralizing antibodies in selected feral and domestic swine sera in the southern united states. | serum samples collected from feral and domestic swine (sus scrofa) in florida and feral swine in georgia and texas were assayed by plaque reduction for their virus neutralizing (vn) antibodies against the porcine transmissible gastroenteritis virus (tge). none of 560 samples collected from feral swine contained vn antibodies for tge virus, but experimentally infected feral swine seroconverted. none of 665 samples from domestic swine contained tge-vn antibodies. these results indicate feral swine ... | 1990 | 2167395 |
| characterization of blood mononuclear cells producing ifn alpha following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus). | porcine blood mononuclear cells (pbmc) were shown to produce interferon-alpha (ifn alpha) following incubation with cells infected by a coronavirus, transmissible gastroenteritis virus. monoclonal antibodies (mab) with specificities for leukocyte subsets and major histocompatibility complex (mhc) antigens were used to characterize ifn alpha producer cells. the production of ifn alpha was found to be a function of non-phagocytic, non-adherent, non-t, non-b, cd4+ (and to a lesser extent cd8+) mhc- ... | 1990 | 2167506 |