Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| efficacy and immunogenicity of a live l. acidophilus expressing sad epitope of transmissible gastroenteritis virus as an oral vaccine. | transmissible gastroenteritis virus (tgev) causes great economic loss to swine industry worldwide. vaccination is an important method to control the tgev infection. in this study, a tgev oral vaccine was generated by transferring a eukaryotic expression recombinant plasmid carrying the sad (a and d antigenic sites of the s protein) epitope of tgev into a swine-origin lactobacillus acidophilus (l. acidophilus). in orally immunized balb/c mice, the tgev l. acidophilus oral vaccine induced signific ... | 2019 | 31507196 |
| coronavirus genomic rna packaging. | rna viruses carry out selective packaging of their genomes in a variety of ways, many involving a genomic packaging signal. the first coronavirus packaging signal was discovered nearly thirty years ago, but how it functions remains incompletely understood. this review addresses the current state of knowledge of coronavirus genome packaging, which has mainly been studied in two prototype species, mouse hepatitis virus and transmissible gastroenteritis virus. despite the progress that has been mad ... | 2019 | 31505321 |
| porcine il-12 plasmid as an adjuvant improves the cellular and humoral immune responses of dna vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model. | transmissible gastroenteritis (tge), caused by transmissible gastroenteritis virus (tgev), is a highly infectious disease in pigs. vaccination is an effective approach to prevent tgev infection. here, we evaluated the potential of tgev s1 as a dna vaccine and porcine interleukin (pil)-12 as an adjuvant in a mouse model. a dna vaccine was constructed with the tgev s1 gene to induce immune response in an experimental mouse model; pil-12 was chosen as the immunological adjuvant within this dna vacc ... | 2019 | 31474664 |
| porcine transmissible gastroenteritis virus inhibits nf-κb activity via nonstructural protein 3 to evade host immune system. | transmissible gastroenteritis virus (tgev), a member of the family coronaviridae, causes lethal watery diarrhea in piglets. previous studies have revealed that the coronaviruses develop various strategies to evade the host innate immunity through the inhibition of nuclear factor kappa b (nf-κb) signaling pathway. however, the ability of tgev to inhibit the host innate immune response by modulating the nf-κb signaling pathway is not clear. | 2019 | 31382996 |
| comparative evaluation of enteric bacterial culture and a molecular multiplex syndromic panel in children with acute gastroenteritis. | although enteric multianalyte syndromic panels are increasingly employed, direct comparisons with traditional methods and the inclusion of host phenotype correlations are limited. luminex xtag gastrointestinal pathogen panel (gpp) and culture results are highly concordant. however, phenotypic and microbiological confirmatory testing raises concerns regarding the accuracy of the gpp, especially for salmonella spp. a total of 3,089 children with gastroenteritis submitted stool specimens, rectal sw ... | 2019 | 30971465 |
| cholesterol 25-hydroxylase negatively regulates porcine intestinal coronavirus replication by the production of 25-hydroxycholesterol. | cholesterol 25-hydroxylase (ch25h) has been shown lately to be a host restriction factor that encodes an enzyme, which catalyzes the oxidized form of cholesterol to 25-hydroxycholesterol (25hc). a series of studies have shown that 25hc activity in hosts plays a vital role in inhibiting viral infection. in this study, we explored the antiviral effect of ch25h and 25hc on porcine epidemic diarrhea virus (pedv), which causes high mortality rates in newborn piglets with severe diarrhea, and consider ... | 2019 | 30955800 |
| multiplex detection of six swine viruses on an integrated centrifugal disk using loop-mediated isothermal amplification. | advances in molecular testing and microfluidic technologies have opened new avenues for rapid detection of animal viruses. we used a centrifugal microfluidic disk (cmfd) to detect 6 important swine viruses, including foot-and-mouth disease virus, classical swine fever virus, porcine reproductive and respiratory swine virus-north american genotype, porcine circovirus 2, pseudorabies virus, and porcine parvovirus. through integrating the loop-mediated isothermal amplification (lamp) method and mic ... | 2019 | 30947641 |
| pigment visibility on rectal swabs used to detect enteropathogens: a prospective cohort study. | data are lacking regarding the impact of visible pigment on rectal swab diagnostic accuracy. we describe the test characteristics of rectal swabs with and without pigment in children with gastroenteritis. between december 2014 and september 2017, children (age, <18 years) with ≥3 episodes of vomiting and/or diarrhea in a 24-h period and symptoms for <7 days were enrolled through two pediatric emergency departments and from a province-wide nursing telephone advice line in alberta, canada. specime ... | 2019 | 30944189 |
| glass wool concentration optimization for the detection of enveloped and non-enveloped waterborne viruses. | an extremely affordable virus concentration method based on adsorption-elution to glass wool and subsequent reconcentration through polyethylene glycol 6000 (peg) precipitation was optimized to recover not only non-enveloped viruses but also enveloped viruses. hepatitis a virus (hav) and transmissible gastroenteritis virus (tgev) were employed as surrogates for naked and enveloped viruses, respectively, to set up the methodology. initial experimentation in small-volume samples showed that both t ... | 2019 | 30903596 |
| simultaneous detection of five pig viruses associated with enteric disease in pigs using evagreen real-time pcr combined with melting curve analysis. | in recent years, a series of porcine diarrhea viruses such as porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), rotaviruses of group a (rva), rotaviruses of group c (rvc), and porcine circovirus 2 (pcv2) caused enormous economic losses all over the world. while any of these viruses is capable to cause disease alone, there is often concurrent infection with more than one virus on pig farms. in this study, a multiplex real-time pcr method based on evagreen fluores ... | 2019 | 30844408 |
| construction and characterization of porcine single-chain fragment variable antibodies that neutralize transmissible gastroenteritis virus in vitro. | transmissible gastroenteritis virus (tgev) infection causes severe diarrhea in piglets and imposes a significant economic burden on pig farms. single-chain fragment variable (scfv) antibodies effectively inhibit virus infection and could be a potential therapeutic reagent for preventing disease. in this study, a recombinant scfv antibody phage display library was constructed from peripheral blood lymphocytes of piglets infected with tgev. the library was screened with four rounds of biopanning u ... | 2019 | 30729994 |
| molecular characteristics of a novel recombinant of porcine epidemic diarrhea virus. | porcine epidemic diarrhea (ped) is a contagious viral disease in pigs, caused by the coronavirus porcine epidemic diarrhea virus (pedv). pedv infection results in significant mortality in piglets in unvaccinated herds. like many others rna viruses, pedv has high evolutionary rate and is prone to genetic mutations. in this study, we analyzed the complete genome sequence of the recently sequenced isolate pedv/belgorod/dom/2008. a recombination event in s gene of pedv/belgorod/dom/2008 was detected ... | 2019 | 30725181 |
| two critical n-terminal epitopes of the nucleocapsid protein contribute to the cross-reactivity between porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus. | both porcine epidemic diarrhoea virus (pedv) and porcine transmissible gastroenteritis virus (tgev), which cause high mortality in piglets and produce similar clinical symptoms and histopathological morphology, belong to the genus alphacoronavirus. serological diagnosis plays an important role in distinguishing pathogen species. together with the spike (s) protein, the nucleocapsid (n) protein is one of the immunodominant regions among coronaviruses. in this study, two-way antigenic cross-reacti ... | 2019 | 30652967 |
| production of porcine aminopeptidase n (papn) site-specific edited pigs. | porcine viral diarrhea is an acute and highly contagious enteric disease in pigs which causes huge economic losses in pig industry worldwide. transmissible gastroenteritis virus (tgev) is main pathogens responsible for piglets viral diarrhea. knockout the host cellular surface receptor for tgev may be an effective way to accelerate the breeding of resistant pigs. in this study, we applied site-specific editing papn which is effective in swine testis (st) cells. site-specific editing of papn redu ... | 2019 | 30623527 |
| evolution of transmissible gastroenteritis virus (tgev): a codon usage perspective. | transmissible gastroenteritis virus (tgev) is a coronavirus associated with diarrhea and high mortality in piglets. to gain insight into the evolution and adaptation of tgev, a comprehensive analysis of phylogeny and codon usage bias was performed. the phylogenetic analyses of maximum likelihood and bayesian inference displayed two distinct genotypes: genotypes i and ii, and genotype i was classified into subtypes ia and ib. the compositional properties revealed that the coding sequence containe ... | 2020 | 33114322 |
| assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine. | transmissible gastroenteritis virus (tgev) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. vaccination is an effective measure to control the disease caused by tgev. | 2020 | 33097081 |
| covid 19 an infectious disease influenced in modern era - recent survey in india. | the virus which is called as corona spoiling many of human lives as well as countries growth. coronavirus is an infectious disease, so spreading from one person to other easily. till now there is no medicine for corona. world health organization (who) taking lot of steps to control it. in india many of them get affected by coronavirus called covid-19. a household survey is conducted nationally through online. this survey is a method for checking knowledge about the infectious virus. in this onli ... | 2020 | 33078095 |
| antiviral effects of bovine antimicrobial peptide against tgev in vivo and in vitro. | in suckling piglets, transmissible gastroenteritis virus (tgev) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. this study explored methods of preventing or inhibiting their production. bovine antimicrobial peptide-13 (apb-13) has antibacterial, antiviral, and immune functions. | 2020 | 33016025 |
| nitazoxanide and jib-04 have broad-spectrum antiviral activity and inhibit sars-cov-2 replication in cell culture and coronavirus pathogenesis in a pig model. | pathogenic coronaviruses represent a major threat to global public health. here, using a recombinant reporter virus-based compound screening approach, we identified several small-molecule inhibitors that potently block the replication of the newly emerged severe acute respiratory syndrome virus 2 (sars-cov-2). two compounds, nitazoxanide and jib-04 inhibited sars-cov-2 replication in vero e6 cells with an ec 50 of 4.90 μm and 0.69 μm, respectively, with specificity indices of greater than 150. b ... | 2020 | 32995798 |
| next-generation porcine intestinal organoids: an apical-out organoid model for swine enteric virus infection and immune response investigations. | the intestinal organoid culture system is a pathbreaking working model for investigating pathogen-host interactions in the intestines. however, due to the limitations of the first generation of intestinal organoids, basal-out structure and growth in matrigel, most pathogens can rarely attach to the apical membrane directly and hardly initiate infection. in this study, we first developed a next-generation porcine intestinal organoid culture system, characterized by an apical membrane on the surfa ... | 2020 | 32796075 |
| dynamics of transmissible gastroenteritis virus internalization unraveled by single-virus tracking in live cells. | transmissible gastroenteritis virus (tgev) is a swine enteropathogenic coronavirus that causes significant economic losses in swine industry. current studies on tgev internalization mainly focus on viral receptors, but the internalization mechanism is still unclear. in this study, we used single-virus tracking to obtain the detailed insights into the dynamic events of the tgev internalization and depict the whole sequential process. we observed that tgevs could be internalized through clathrin- ... | 2020 | 32017270 |
| transmissible gastroenteritis virus targets paneth cells to inhibit the self-renewal and differentiation of lgr5 intestinal stem cells via notch signaling. | infection with transmissible gastroenteritis virus (tgev) has been associated with villous atrophy within 48 h, which seriously disrupts intestinal homeostasis. however, the underlying mechanisms remain elusive. in this study, we found that tgev infection severely disrupted intestinal homeostasis via inhibition of self-renewal and differentiation in lgr5 intestinal stem cells (iscs). profoundly, tgev-encoded nsp10/nsp16 protein complex-mediated the inactivation of notch signaling provided a mech ... | 2020 | 31959773 |
| molecular diversity of alphacoronavirus 1 in dogs and cats in colombia. | alphacoronavirus 1 (subgenus tegacovirus, genus alphacoronavirus, family coronaviridae), which encompasses transmissible gastroenteritis virus (tgev), feline coronavirus (fcov) and canine coronavirus (ccov), is an important pathogen that can cause severe gastroenteritis and is distributed worldwide. ccov has two different genotypes: ccov type i, which has a high identity with fcov-i, and ccov type ii, which is divided into two subtypes, ccov iia (pantropic) and ccov iib, which is related to fcov ... | 2020 | 32665984 |
| microrna-4331-5p promotes fmdv replication through inhibiting interferon pathways in pk-15 cells. | micrornas play vital roles in regulating the battle between pathogens and host cells during viral challenging. mir-4331 aggravates transmissible gastroenteritis virus (tgev) -induced mitochondrial damage, also suppresses transcription of tgev gene 7 via targeting cellular cdca7. otherwise, mir-4331-5p affects h1n1/2009 influenza a virus replication by targeting viral ha and ns. however, whether microrna ssc-mir-4331-5p (mir-4331-5p) regulates foot and mouth virus (fmdv) replication remains uncle ... | 2020 | 32574680 |
| novel sars-cov-2/covid-19: origin, pathogenesis, genes and genetic variations, immune responses and phylogenetic analysis. | in this review, we focused on the origins of the novel coronavirus (sars-cov-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing broadly effective preventions and vaccines to cope covid-19. towards the end of 2019, sars-cov-2 has emerged in association with the sars, later was named covid-19 caused an environment of chaos worldwide and infected a massive number of lives. since ... | 2020 | 32566803 |
| role of porcine aminopeptidase n and sialic acids in porcine coronavirus infections in primary porcine enterocytes. | porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) have been reported to use aminopeptidase n (apn) as a cellular receptor. recently, the role of apn as a receptor for pedv has been questioned. in our study, the role of apn in pedv and tgev infections was studied in primary porcine enterocytes. after seven days of cultivation, 89% of enterocytes presented microvilli and showed a two- to five-fold higher susceptibility to pedv and tgev. a significant increase of ... | 2020 | 32260595 |
| characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in china. | porcine transmissible gastroenteritis virus (tgev) is one of the major etiological agents of viral enteritis and fetal diarrhea in suckling piglets. in this study, a tgev js2012 strain was isolated from the feces of piglets in jiangsu province, china. the phylogenetic analysis showed that tgev js2012 was placed between the purdue and the miller clusters. analysis of recombination confirmed that tgev js2012 is a natural recombinant strain between miller m6 and purdue 115. similar to miller m6, vi ... | 2020 | 32174456 |
| establishment of method for dual simultaneous detection of pedv and tgev by combination of magnetic micro-particles and nanoparticles. | transmissible gastroenteritis virus (tgev) and porcine epidemic diarrhea virus (pedv) are the main pathogens causing viral diarrhea in pig, mixed infections of these two viruses are very common in intensive pig rearing. however, there is a lack of a method to simultaneously detect and distinguish pedv and tgev in preclinical levels. in this study, we aimed to establish a dual ultrasensitive nanoparticle dna probe-based pcr assay (dual undp-pcr) based on functionalized magnetic bead enrichment an ... | 2020 | 32147375 |
| identification and characterization of linear b cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies. | the nucleocapsid (n) protein of porcine epidemic diarrhea virus (pedv), the most important pathogen causing severe diarrhea in piglets, is a highly conserved structural protein. in this study, 5 monoclonal antibodies (mcabs) against the pedv n-protein were prepared and identified. three new epitopes, 56qirwrmrrgeri67, 318gyaqiaslapnvaallfggnva vre342 and 398heeaiyddv406, were firstly identified in the viral n-protein, by using mcabs 3f10, 6a11, and 1c9. the epitope 398heeaiyddv406 was deleted in ... | 2020 | 32142743 |
| structural basis for inhibiting porcine epidemic diarrhea virus replication with the 3c-like protease inhibitor gc376. | porcine epidemic diarrhea virus (pedv), being highly virulent and contagious in piglets, has caused significant damage to the pork industries of many countries worldwide. there are no commercial drugs targeting coronaviruses (covs), and few studies on anti-pedv inhibitors. the coronavirus 3c-like protease (3clpro) has a conserved structure and catalytic mechanism and plays a key role during viral polyprotein processing, thus serving as an appealing antiviral drug target. here, we report the anti ... | 2020 | 32098094 |
| biological and antigenic relationships between virus-induced porcine and human interferons. | endogenous interferon (ifn) made by the newborn piglet in response to enteric coronavirus tgev (transmissible gastroenteritis virus) infection was identified as leukocytic ifn (ifn-α). indeed, the antiviral activity found in the serum and in the urine of infected piglets displayed the same main biological and antigenic properties as the ifn induced in influenza-infected pig leukocytes. it is therefore concluded that most, if not all, circulating ifn activity must be derived from lymphoid cells. ... | 2020 | 32288182 |
| prevalence and genomic characteristics of a novel reassortment mammalian orthoreovirus type 2 in diarrhea piglets in sichuan, china. | mammalian orthoreovirus (mrv), which can infect almost all mammals, is a zoonotic virus. in this study, six strains of type 2 mrv (mrv2) were isolated from 7 diarrhea piglets from a farm that had an outbreak of diarrhea in piglets in 2018, which tested negative for porcine epidemic diarrhea virus (pedv), porcine transmissible gastroenteritis virus (tgev), porcine deltacoronavirus (pdcov), and porcine group a rotavirus (rav). the mrv2 isolate infected neonatal piglets, in which it induced severe ... | 2020 | 32544614 |
| postweaning mortality in commercial swine production ii: review of infectious contributing factors. | postweaning mortality is extremely complex with a multitude of noninfectious and infectious contributing factors. in the current review, our objective is to describe the current state of knowledge regarding infectious causes of postweaning mortality, focusing on estimates of frequency and magnitude of effect where available. while infectious mortality is often categorized by physiologic body system affected, we believe the complex multifactorial nature is better understood by an alternative stra ... | 2020 | 32705048 |
| surface-displayed porcine ifn-λ3 in lactobacillus plantarum inhibits porcine enteric coronavirus infection of porcine intestinal epithelial cells. | interferon (ifn)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. lactobacillus plantarum (l. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. alphacoronaviruses, especially porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev), cause high morbidity and mortality in piglets resulting in economic loss. co-infection by these two viruses is becoming increasingly ... | 2020 | 31838830 |
| application of nanomaterials in treatment, anti-infection and detection of coronaviruses. | nanotechnology and nanomedicine have excellent potential in dealing with a range of different health problems, including viruses, which are considered to be a serious challenge in the medical field. application of nanobiotechnology could represent a new avenue for the treatment or disinfection of viruses. there is increasing concern regarding the control of coronaviruses, among these, middle east respiratory syndrome coronavirus, severe acute respiratory syndrome coronavirus and severe acute res ... | 2020 | 32378459 |
| development of a multiplex rt-pcr for the detection of major diarrhoeal viruses in pig herds in china. | the major enteric rna viruses in pigs include porcine epidemic diarrhoea virus (pedv), transmissible gastroenteritis virus (tgev), porcine rotavirus a (prv-a), porcine kobuvirus (pkv), porcine sapovirus (psav) and porcine deltacoronavirus (pdcov). for differential diagnosis, a multiplex rt-pcr method was established on the basis of the n genes of tgev, pedv and pdcov, the vp7 gene of prv-a, and the polyprotein genes of pkv and psav. this multiplex rt-pcr could specifically detect tgev, pedv, pdc ... | 2020 | 31597013 |
| rhodanine derivative lj001 inhibits tgev and pdcov replication in vitro. | transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) are members of the family coronaviridae and mainly cause acute diarrhea/vomiting, dehydration and mortality in piglets, which lead to huge economic losses to the swine industry. rhodanine derivative lj001 has been verified to be effective against some enveloped virus infections in vitro. in this study, we evaluated the antiviral activity of lj001 towards tgev and pdcov replication on swine testicular(st) cells. our r ... | 2020 | 32956749 |
| decreased nhe3 activity and trafficking in tgev-infected ipec-j2 cells via the sglt1-mediated p38 mapk/akt2 pathway. | transmissible gastroenteritis virus (tgev) primarily replicates in intestinal epithelial cells and causes severe damage to host cells, resulting in diarrhea. surface nhe3 serves as the key regulatory site controlling electroneutral na+ absorption. in this study, our results showed that the surface nhe3 content was significantly reduced following tgev infection, whereas the total level of protein expression was not significantly changed, and nhe3 activity gradually decreased with prolonged infect ... | 2020 | 32070687 |
| genus-specific pattern of intrinsically disordered central regions in the nucleocapsid protein of coronaviruses. | the nucleocapsid (n) protein is conserved in all four genera of the coronaviruses, namely alpha, beta, gamma, and delta, and is essential for genome functionality. bioinformatic analysis of coronaviral n sequences revealed two intrinsically disordered regions (idrs) at the center of the polypeptide. while both idr structures were found in alpha, beta, and gamma-coronaviruses, the second idr was absent in deltacoronaviruses. two novel coronaviruses, currently placed in the gammacoronavirus genus, ... | 2020 | 32765822 |
| antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro. | emerging coronaviruses represent serious threats to human and animal health worldwide, and no approved therapeutics are currently available. here, we used transmissible gastroenteritis virus (tgev) as the alpha-coronavirus model, and investigated the antiviral properties of curcumin against tgev. our results demonstrated that curcumin strongly inhibited tgev proliferation and viral protein expression in a dose-dependent manner. we also observed that curcumin exhibited direct virucidal abilities ... | 2020 | 32677610 |
| agrodiag porcov: a multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses. | three different porcine enteric coronaviruses (pecs), i.e., porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) are currently circulating in u.s. commercial swine herds. differential diagnosis of pecs relies on laboratory methods. this study describes the development of an elisa-like multiplex planar immunoassay based on virus-specific recombinant s1 proteins printed in an array of spots at the bottom of a 96-well microplate for ... | 2020 | 32562689 |
| rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick. | porcine deltacoronavirus (pdcov) is a newly emerging coronavirus that was first identified in 2012 in hong kong, china. since then, pdcov has subsequently been reported worldwide, causing a high number of neonatal piglet deaths and significant economic losses to the swine industry. therefore, it is necessary to establish a highly sensitive and specific method for the rapid diagnosis of pdcov. | 2020 | 32381014 |
| detecting and monitoring porcine hemagglutinating encephalomyelitis virus, an underresearched betacoronavirus. | members of family coronaviridae cause a variety of diseases in birds and mammals. porcine hemagglutinating encephalomyelitis virus (phev), a lesser-researched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs ≤4 weeks of age. no commercial phev vaccines are available, and neonatal protection from phev-associated disease is presumably dependent on lactogenic immunity. although subclinical phev infections are thought to be common, phev ecology in commercial sw ... | 2020 | 32376700 |
| the use of cells from anpep knockout pigs to evaluate the role of aminopeptidase n (apn) as a receptor for porcine deltacoronavirus (pdcov). | the coronaviruses, porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine deltacoronavirus (pdcov) represent important sources of neonatal diarrhea on pig farms. the requirement for aminopeptidase n (apn) as a receptor for tgev, but not for pedv, is well established. in this study, the biological relevance of apn as a receptor for pdcov was tested by using crispr/cas9 to knockout the apn gene, anpep, in pigs. porcine alveolar macrophages (pams) from anpep ... | 2020 | 32056711 |
| cd163 and papn double-knockout pigs are resistant to prrsv and tgev and exhibit decreased susceptibility to pdcov while maintaining normal production performance. | porcine reproductive and respiratory syndrome virus (prrsv) and transmissible gastroenteritis virus (tgev) are two highly infectious and lethal viruses causing major economic losses to pig production. here, we report generation of double-gene-knockout (dko) pigs harboring edited knockout alleles for known receptor proteins cd163 and papn and show that dko pigs are completely resistant to genotype 2 prrsv and tgev. we found no differences in meat-production or reproductive-performance traits betw ... | 2020 | 32876563 |
| receptor tyrosine kinase inhibitors block proliferation of tgev mainly through p38 mitogen-activated protein kinase pathways. | emerging coronaviruses (covs) primarily cause severe gastroenteric or respiratory diseases in humans and animals, and no approved therapeutics are currently available. here, a9, a receptor tyrosine kinase inhibitor (rtki) of the tyrphostin class, is identified as a robust inhibitor of transmissible gastroenteritis virus (tgev) infection in cell-based assays. moreover, a9 exhibited potent antiviral activity against the replication of various covs, including murine hepatitis virus (mhv), porcine e ... | 2020 | 31751591 |
| nucleocapsid proteins from other swine enteric coronaviruses differentially modulate pedv replication. | porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) share tropism for swine intestinal epithelial cells. whether mixing of viral components during co-infection alters pathogenic outcomes or viral replication is not known. in this study, we investigated how different coronavirus nucleocapsid (cov n) proteins interact and affect pedv replication. we found that pdcov n and tgev n can competitively interact with pedv n. however, the ... | 2020 | 31756532 |
| inhibition of transmissible gastroenteritis coronavirus (tgev) multiplication in vitro by non-immune lymphocytes. | in vitro studies were undertaken to examine the effects of non-immune porcine peripheral blood leukocytes (pbl) on a coronavirus infection due to transmissible gastroenteritis virus (tgev). the assay consisted of tgev-infected epithelial cells expressing viral antigens on the cell surface and producing low amounts of interferon (ifn). non-immune pbl were found to limit virus replication at an effector-to-target ratio of 100/1 even when effector cells were depleted of phagocytic cells. neutralizi ... | 2020 | 32288183 |
| production of monoclonal antibodies targeting aminopeptidase n in the porcine intestinal mucosal epithelium. | porcine aminopeptidase n (apn), a membrane-bound metallopeptidase abundantly present in small intestinal mucosa, can initiate a mucosal immune response without any interference such as low protein expression, enzyme inactivity, or structural changes. this makes apn an attractive candidate in the development of vaccines that selectively target the mucosal epithelium. previous studies have shown that apn is a receptor protein for both enterotoxigenic escherichia coli (e. coli) f4 and transmissible ... | 2021 | 34096912 |
| sars-cov-2 in environmental perspective: occurrence, persistence, surveillance, inactivation and challenges. | the unprecedented global spread of the severe acute respiratory syndrome (sars) caused by sars-cov-2 is depicting the distressing pandemic consequence on human health, economy as well as ecosystem services. so far novel coronavirus (cov) outbreaks were associated with sars-cov-2 (2019), middle east respiratory syndrome coronavirus (mers-cov, 2012), and sars-cov-1 (2003) events. cov relates to the enveloped family of betacoronavirus (βcov) with positive-sense single-stranded rna (+ssrna). knowing ... | 2021 | 32901196 |