Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| transmissible gastroenteritis virus infection up-regulates fcrn expression via nucleocapsid protein and secretion of tgf-β in porcine intestinal epithelial cells. | transmissible gastroenteritis virus (tgev) is a porcine intestinal coronavirus that causes fatal severe watery diarrhea in piglets. the neonatal fc receptor (fcrn) is the only igg transport receptor, its expression on mucosal surfaces is triggered upon viral stimulation, which significantly enhances mucosal immunity. we utilized tgev as a model pathogen to explore the role of fcrn in resisting viral invasion in overall intestinal mucosal immunity. tgev induced fcrn expression by activating nf-κb ... | 2019 | 32038538 |
| minimum determinants of transmissible gastroenteritis virus enteric tropism are located in the n-terminus of spike protein. | transmissible gastroenteritis virus (tgev) is an enteric coronavirus causing high morbidity and mortality in porcine herds worldwide, that possesses both enteric and respiratory tropism. the ability to replicate in the enteric tract directly correlates with virulence, as tgevs with an exclusive respiratory tropism are attenuated. the tissue tropism is determined by spike (s) protein, although the molecular bases for enteric tropism remain to be fully characterized. both papn and sialic acid bind ... | 2019 | 31861369 |
| prevalence and phylogenetic analysis of porcine diarrhea associated viruses in southern china from 2012 to 2018. | in china, large-scale outbreaks of severe diarrhea caused by viruses have occurred in pigs since late 2010. to investigate the prevalence and genetic evolution of diarrhea-associated viruses responsible for the outbreaks, a total of 2987 field diarrheal samples collected from 168 pig farms in five provinces in southern china during 2012-2018 were tested. | 2019 | 31881873 |
| a conserved region of nonstructural protein 1 from alphacoronaviruses inhibits host gene expression and is critical for viral virulence. | coronaviruses are enveloped, single-stranded rna viruses that are distributed worldwide. they include transmissible gastroenteritis virus (tgev), porcine epidemic diarrhea virus (pedv), and the human coronaviruses severe acute respiratory syndrome coronavirus (sars-cov) and middle east respiratory syndrome coronavirus (mers-cov), many of which seriously endanger human health and well-being. only alphacoronaviruses and betacoronaviruses harbor nonstructural protein 1 (nsp1), which performs multip ... | 2019 | 31350335 |
| retrospective detection and phylogenetic analysis of swine acute diarrhoea syndrome coronavirus in pigs in southern china. | swine acute diarrhoea syndrome coronavirus (sads-cov), a novel coronavirus, was first discovered in southern china in january 2017 and caused a large scale outbreak of fatal diarrheal disease in piglets. here, we conducted a retrospective investigation of 236 samples from 45 swine farms with a clinical history of diarrheal disease to evaluate the emergence and the distribution of sads-cov in pigs in china. our results suggest that sads-cov has emerged in china at least since august 2016. meanwhi ... | 2019 | 30171801 |
| prevalence of antibodies against transmissible gastroenteritis virus (tgev) in hungary. | transmissible gastroenteritis (tge) is a highly contagious enteric disease of swine, which became infrequent with the appearance of porcine respiratory coronavirus (prcv). tge was last reported in hungary in 2013 and the virus has not been found since, therefore a serological survey was planned to estimate the level of protection against it. 908 sera of sows from 93 farms were selected together with 174 archive samples from one farm covering a wider age group. all samples were screened with an i ... | 2019 | 32289094 |
| the n-terminal domain of spike protein is not the enteric tropism determinant for transmissible gastroenteritis virus in piglets. | transmissible gastroenteritis virus (tgev) is the etiologic agent of transmissible gastroenteritis in pigs, and the n-terminal domain of tgev spike protein is generally recognized as both the virulence determinant and enteric tropism determinant. here, we assembled a full-length infectious cdna clone of tgev in a bacterial artificial chromosome. using a novel approach, the clustered regularly interspaced short palindromic repeat (crispr)/crispr-associated protein 9 (cas9) systems efficiently and ... | 2019 | 30935078 |
| evaluation of the serologic cross-reactivity between transmissible gastroenteritis coronavirus and porcine respiratory coronavirus using commercial blocking enzyme-linked immunosorbent assay kits. | this study compared the performances of three commercial transmissible gastroenteritis virus/porcine respiratory coronavirus (tgev/prcv) blocking enzyme-linked immunosorbent assays (elisas) using serum samples (n = 528) collected over a 49-day observation period from pigs inoculated with tgev strain purdue (n = 12), tgev strain miller (n = 12), prcv (n = 12), or with virus-free culture medium (n = 12). elisa results were evaluated both with "suspect" results interpreted as positive and then as n ... | 2019 | 30867325 |
| decline of transmissible gastroenteritis virus and its complex evolutionary relationship with porcine respiratory coronavirus in the united states. | the epidemiology and genetic diversity of transmissible gastroenteritis virus (tgev) in the united states (us) was investigated by testing clinical cases for tgev by real time rt-pcr between january 2008 and november 2016. prevalence of tgev ranged between 3.8-6.8% and peaked during cold months until march 2013, in which prevalence decreased to < 0.1%. nineteen complete tgev genomes and a single strain of porcine respiratory coronavirus (prcv) from the us were generated and compared to historica ... | 2019 | 30850666 |
| prospects for the use of spherical gold nanoparticles in immunization. | recent years have seen extremely fast development of new viral nanovaccines and diagnostic agents using nanostructures prepared by biological and chemical synthesis. we used spherical gold nanoparticles (average diameter, 15 nm) as a platform for the antigen for swine transmissible gastroenteritis virus (tgev). the literature data demonstrate that immunization of animals with the tgev antigen coupled to gold nanoparticles (gnps) not only activates antigen-presenting cells but also increases the ... | 2019 | 30402771 |
| interferon gamma inhibits transmissible gastroenteritis virus infection mediated by an irf1 signaling pathway. | interferon gamma (ifn-γ) is best known for its ability to regulate host immune responses; however, its direct antiviral activity is less well studied. transmissible gastroenteritis virus (tgev) is an economically important swine enteric coronavirus and causes acute diarrhea in piglets. at present, little is known about the function of ifn-γ in the control of tgev infection. in this study, we demonstrated that ifn-γ inhibited tgev infection directly in st cells and intestine epithelial ipec-j2 ce ... | 2019 | 31385116 |
| recombinant chimeric transmissible gastroenteritis virus (tgev) - porcine epidemic diarrhea virus (pedv) virus provides protection against virulent pedv. | porcine epidemic diarrhea virus (pedv) is an enteric coronavirus causing high morbidity and mortality in porcine herds worldwide. although both inactivated and live attenuated vaccines have been extensively used, the emergence of highly virulent strains and the recurrent outbreaks even in vaccinated farms highlight the need of effective vaccines. engineering of genetically defined live attenuated vaccines is a rational approach for novel vaccine development. in this line, we engineered an attenu ... | 2019 | 31349683 |
| susceptibility of porcine ipi-2i intestinal epithelial cells to infection with swine enteric coronaviruses. | swine enteric coronavirus (cov) is an important group of pathogens causing diarrhea in piglets. at least four kinds of swine enteric covs have been identified, including transmissible gastroenteritis virus (tgev), porcine epidemic diarrhea virus (pedv), porcine deltacoronavirus (pdcov), and the emerging hku2-like porcine enteric alphacoronavirus (peav). the small intestines, particularly the jejunum and ileum, are the most common targets of these four covs in vivo, and co-infections by these cov ... | 2019 | 31176408 |
| a taqman-probe-based multiplex real-time rt-qpcr for simultaneous detection of porcine enteric coronaviruses. | swine enteric coronaviruses are a group of most significant pathogens causing diarrhea in piglets with similar clinical symptoms and pathological changes. to develop a simple, rapid, accurate, and high-throughput detection method for diagnosis and differential diagnosis on swine enteric coronaviruses, specific primers and probes were designed based on the highly conserved regions of transmissible gastroenteritis virus (tgev) n, porcine epidemic diarrhea virus (pedv) m, porcine deltacoronavirus ( ... | 2019 | 31025076 |
| biological and antigenic relationships between virus-induced porcine and human interferons. | endogenous interferon (ifn) made by the newborn piglet in response to enteric coronavirus tgev (transmissible gastroenteritis virus) infection was identified as leukocytic ifn (ifn-α). indeed, the antiviral activity found in the serum and in the urine of infected piglets displayed the same main biological and antigenic properties as the ifn induced in influenza-infected pig leukocytes. it is therefore concluded that most, if not all, circulating ifn activity must be derived from lymphoid cells. ... | 2020 | 32288182 |
| application of nanomaterials in treatment, anti-infection and detection of coronaviruses. | nanotechnology and nanomedicine have excellent potential in dealing with a range of different health problems, including viruses, which are considered to be a serious challenge in the medical field. application of nanobiotechnology could represent a new avenue for the treatment or disinfection of viruses. there is increasing concern regarding the control of coronaviruses, among these, middle east respiratory syndrome coronavirus, severe acute respiratory syndrome coronavirus and severe acute res ... | 2020 | 32378459 |
| prevalence and genomic characteristics of a novel reassortment mammalian orthoreovirus type 2 in diarrhea piglets in sichuan, china. | mammalian orthoreovirus (mrv), which can infect almost all mammals, is a zoonotic virus. in this study, six strains of type 2 mrv (mrv2) were isolated from 7 diarrhea piglets from a farm that had an outbreak of diarrhea in piglets in 2018, which tested negative for porcine epidemic diarrhea virus (pedv), porcine transmissible gastroenteritis virus (tgev), porcine deltacoronavirus (pdcov), and porcine group a rotavirus (rav). the mrv2 isolate infected neonatal piglets, in which it induced severe ... | 2020 | 32544614 |
| cd163 and papn double-knockout pigs are resistant to prrsv and tgev and exhibit decreased susceptibility to pdcov while maintaining normal production performance. | porcine reproductive and respiratory syndrome virus (prrsv) and transmissible gastroenteritis virus (tgev) are two highly infectious and lethal viruses causing major economic losses to pig production. here, we report generation of double-gene-knockout (dko) pigs harboring edited knockout alleles for known receptor proteins cd163 and papn and show that dko pigs are completely resistant to genotype 2 prrsv and tgev. we found no differences in meat-production or reproductive-performance traits betw ... | 2020 | 32876563 |
| receptor tyrosine kinase inhibitors block proliferation of tgev mainly through p38 mitogen-activated protein kinase pathways. | emerging coronaviruses (covs) primarily cause severe gastroenteric or respiratory diseases in humans and animals, and no approved therapeutics are currently available. here, a9, a receptor tyrosine kinase inhibitor (rtki) of the tyrphostin class, is identified as a robust inhibitor of transmissible gastroenteritis virus (tgev) infection in cell-based assays. moreover, a9 exhibited potent antiviral activity against the replication of various covs, including murine hepatitis virus (mhv), porcine e ... | 2020 | 31751591 |
| evolution of transmissible gastroenteritis virus (tgev): a codon usage perspective. | transmissible gastroenteritis virus (tgev) is a coronavirus associated with diarrhea and high mortality in piglets. to gain insight into the evolution and adaptation of tgev, a comprehensive analysis of phylogeny and codon usage bias was performed. the phylogenetic analyses of maximum likelihood and bayesian inference displayed two distinct genotypes: genotypes i and ii, and genotype i was classified into subtypes ia and ib. the compositional properties revealed that the coding sequence containe ... | 2020 | 33114322 |
| assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine. | transmissible gastroenteritis virus (tgev) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. vaccination is an effective measure to control the disease caused by tgev. | 2020 | 33097081 |
| covid 19 an infectious disease influenced in modern era - recent survey in india. | the virus which is called as corona spoiling many of human lives as well as countries growth. coronavirus is an infectious disease, so spreading from one person to other easily. till now there is no medicine for corona. world health organization (who) taking lot of steps to control it. in india many of them get affected by coronavirus called covid-19. a household survey is conducted nationally through online. this survey is a method for checking knowledge about the infectious virus. in this onli ... | 2020 | 33078095 |
| antiviral effects of bovine antimicrobial peptide against tgev in vivo and in vitro. | in suckling piglets, transmissible gastroenteritis virus (tgev) causes lethal diarrhea accompanied by high infection and mortality rates, leading to considerable economic losses. this study explored methods of preventing or inhibiting their production. bovine antimicrobial peptide-13 (apb-13) has antibacterial, antiviral, and immune functions. | 2020 | 33016025 |
| nitazoxanide and jib-04 have broad-spectrum antiviral activity and inhibit sars-cov-2 replication in cell culture and coronavirus pathogenesis in a pig model. | pathogenic coronaviruses represent a major threat to global public health. here, using a recombinant reporter virus-based compound screening approach, we identified several small-molecule inhibitors that potently block the replication of the newly emerged severe acute respiratory syndrome virus 2 (sars-cov-2). two compounds, nitazoxanide and jib-04 inhibited sars-cov-2 replication in vero e6 cells with an ec 50 of 4.90 μm and 0.69 μm, respectively, with specificity indices of greater than 150. b ... | 2020 | 32995798 |
| next-generation porcine intestinal organoids: an apical-out organoid model for swine enteric virus infection and immune response investigations. | the intestinal organoid culture system is a pathbreaking working model for investigating pathogen-host interactions in the intestines. however, due to the limitations of the first generation of intestinal organoids, basal-out structure and growth in matrigel, most pathogens can rarely attach to the apical membrane directly and hardly initiate infection. in this study, we first developed a next-generation porcine intestinal organoid culture system, characterized by an apical membrane on the surfa ... | 2020 | 32796075 |
| genus-specific pattern of intrinsically disordered central regions in the nucleocapsid protein of coronaviruses. | the nucleocapsid (n) protein is conserved in all four genera of the coronaviruses, namely alpha, beta, gamma, and delta, and is essential for genome functionality. bioinformatic analysis of coronaviral n sequences revealed two intrinsically disordered regions (idrs) at the center of the polypeptide. while both idr structures were found in alpha, beta, and gamma-coronaviruses, the second idr was absent in deltacoronaviruses. two novel coronaviruses, currently placed in the gammacoronavirus genus, ... | 2020 | 32765822 |
| antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro. | emerging coronaviruses represent serious threats to human and animal health worldwide, and no approved therapeutics are currently available. here, we used transmissible gastroenteritis virus (tgev) as the alpha-coronavirus model, and investigated the antiviral properties of curcumin against tgev. our results demonstrated that curcumin strongly inhibited tgev proliferation and viral protein expression in a dose-dependent manner. we also observed that curcumin exhibited direct virucidal abilities ... | 2020 | 32677610 |
| molecular diversity of alphacoronavirus 1 in dogs and cats in colombia. | alphacoronavirus 1 (subgenus tegacovirus, genus alphacoronavirus, family coronaviridae), which encompasses transmissible gastroenteritis virus (tgev), feline coronavirus (fcov) and canine coronavirus (ccov), is an important pathogen that can cause severe gastroenteritis and is distributed worldwide. ccov has two different genotypes: ccov type i, which has a high identity with fcov-i, and ccov type ii, which is divided into two subtypes, ccov iia (pantropic) and ccov iib, which is related to fcov ... | 2020 | 32665984 |
| microrna-4331-5p promotes fmdv replication through inhibiting interferon pathways in pk-15 cells. | micrornas play vital roles in regulating the battle between pathogens and host cells during viral challenging. mir-4331 aggravates transmissible gastroenteritis virus (tgev) -induced mitochondrial damage, also suppresses transcription of tgev gene 7 via targeting cellular cdca7. otherwise, mir-4331-5p affects h1n1/2009 influenza a virus replication by targeting viral ha and ns. however, whether microrna ssc-mir-4331-5p (mir-4331-5p) regulates foot and mouth virus (fmdv) replication remains uncle ... | 2020 | 32574680 |
| role of porcine aminopeptidase n and sialic acids in porcine coronavirus infections in primary porcine enterocytes. | porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) have been reported to use aminopeptidase n (apn) as a cellular receptor. recently, the role of apn as a receptor for pedv has been questioned. in our study, the role of apn in pedv and tgev infections was studied in primary porcine enterocytes. after seven days of cultivation, 89% of enterocytes presented microvilli and showed a two- to five-fold higher susceptibility to pedv and tgev. a significant increase of ... | 2020 | 32260595 |
| characterization and evaluation of the pathogenicity of a natural recombinant transmissible gastroenteritis virus in china. | porcine transmissible gastroenteritis virus (tgev) is one of the major etiological agents of viral enteritis and fetal diarrhea in suckling piglets. in this study, a tgev js2012 strain was isolated from the feces of piglets in jiangsu province, china. the phylogenetic analysis showed that tgev js2012 was placed between the purdue and the miller clusters. analysis of recombination confirmed that tgev js2012 is a natural recombinant strain between miller m6 and purdue 115. similar to miller m6, vi ... | 2020 | 32174456 |
| establishment of method for dual simultaneous detection of pedv and tgev by combination of magnetic micro-particles and nanoparticles. | transmissible gastroenteritis virus (tgev) and porcine epidemic diarrhea virus (pedv) are the main pathogens causing viral diarrhea in pig, mixed infections of these two viruses are very common in intensive pig rearing. however, there is a lack of a method to simultaneously detect and distinguish pedv and tgev in preclinical levels. in this study, we aimed to establish a dual ultrasensitive nanoparticle dna probe-based pcr assay (dual undp-pcr) based on functionalized magnetic bead enrichment an ... | 2020 | 32147375 |
| identification and characterization of linear b cell epitopes on the nucleocapsid protein of porcine epidemic diarrhea virus using monoclonal antibodies. | the nucleocapsid (n) protein of porcine epidemic diarrhea virus (pedv), the most important pathogen causing severe diarrhea in piglets, is a highly conserved structural protein. in this study, 5 monoclonal antibodies (mcabs) against the pedv n-protein were prepared and identified. three new epitopes, 56qirwrmrrgeri67, 318gyaqiaslapnvaallfggnva vre342 and 398heeaiyddv406, were firstly identified in the viral n-protein, by using mcabs 3f10, 6a11, and 1c9. the epitope 398heeaiyddv406 was deleted in ... | 2020 | 32142743 |
| structural basis for inhibiting porcine epidemic diarrhea virus replication with the 3c-like protease inhibitor gc376. | porcine epidemic diarrhea virus (pedv), being highly virulent and contagious in piglets, has caused significant damage to the pork industries of many countries worldwide. there are no commercial drugs targeting coronaviruses (covs), and few studies on anti-pedv inhibitors. the coronavirus 3c-like protease (3clpro) has a conserved structure and catalytic mechanism and plays a key role during viral polyprotein processing, thus serving as an appealing antiviral drug target. here, we report the anti ... | 2020 | 32098094 |
| decreased nhe3 activity and trafficking in tgev-infected ipec-j2 cells via the sglt1-mediated p38 mapk/akt2 pathway. | transmissible gastroenteritis virus (tgev) primarily replicates in intestinal epithelial cells and causes severe damage to host cells, resulting in diarrhea. surface nhe3 serves as the key regulatory site controlling electroneutral na+ absorption. in this study, our results showed that the surface nhe3 content was significantly reduced following tgev infection, whereas the total level of protein expression was not significantly changed, and nhe3 activity gradually decreased with prolonged infect ... | 2020 | 32070687 |
| rhodanine derivative lj001 inhibits tgev and pdcov replication in vitro. | transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) are members of the family coronaviridae and mainly cause acute diarrhea/vomiting, dehydration and mortality in piglets, which lead to huge economic losses to the swine industry. rhodanine derivative lj001 has been verified to be effective against some enveloped virus infections in vitro. in this study, we evaluated the antiviral activity of lj001 towards tgev and pdcov replication on swine testicular(st) cells. our r ... | 2020 | 32956749 |
| postweaning mortality in commercial swine production ii: review of infectious contributing factors. | postweaning mortality is extremely complex with a multitude of noninfectious and infectious contributing factors. in the current review, our objective is to describe the current state of knowledge regarding infectious causes of postweaning mortality, focusing on estimates of frequency and magnitude of effect where available. while infectious mortality is often categorized by physiologic body system affected, we believe the complex multifactorial nature is better understood by an alternative stra ... | 2020 | 32705048 |
| agrodiag porcov: a multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses. | three different porcine enteric coronaviruses (pecs), i.e., porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) are currently circulating in u.s. commercial swine herds. differential diagnosis of pecs relies on laboratory methods. this study describes the development of an elisa-like multiplex planar immunoassay based on virus-specific recombinant s1 proteins printed in an array of spots at the bottom of a 96-well microplate for ... | 2020 | 32562689 |
| rapid and visual detection of porcine deltacoronavirus by recombinase polymerase amplification combined with a lateral flow dipstick. | porcine deltacoronavirus (pdcov) is a newly emerging coronavirus that was first identified in 2012 in hong kong, china. since then, pdcov has subsequently been reported worldwide, causing a high number of neonatal piglet deaths and significant economic losses to the swine industry. therefore, it is necessary to establish a highly sensitive and specific method for the rapid diagnosis of pdcov. | 2020 | 32381014 |
| detecting and monitoring porcine hemagglutinating encephalomyelitis virus, an underresearched betacoronavirus. | members of family coronaviridae cause a variety of diseases in birds and mammals. porcine hemagglutinating encephalomyelitis virus (phev), a lesser-researched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs ≤4 weeks of age. no commercial phev vaccines are available, and neonatal protection from phev-associated disease is presumably dependent on lactogenic immunity. although subclinical phev infections are thought to be common, phev ecology in commercial sw ... | 2020 | 32376700 |
| the use of cells from anpep knockout pigs to evaluate the role of aminopeptidase n (apn) as a receptor for porcine deltacoronavirus (pdcov). | the coronaviruses, porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine deltacoronavirus (pdcov) represent important sources of neonatal diarrhea on pig farms. the requirement for aminopeptidase n (apn) as a receptor for tgev, but not for pedv, is well established. in this study, the biological relevance of apn as a receptor for pdcov was tested by using crispr/cas9 to knockout the apn gene, anpep, in pigs. porcine alveolar macrophages (pams) from anpep ... | 2020 | 32056711 |
| nucleocapsid proteins from other swine enteric coronaviruses differentially modulate pedv replication. | porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev) and porcine deltacoronavirus (pdcov) share tropism for swine intestinal epithelial cells. whether mixing of viral components during co-infection alters pathogenic outcomes or viral replication is not known. in this study, we investigated how different coronavirus nucleocapsid (cov n) proteins interact and affect pedv replication. we found that pdcov n and tgev n can competitively interact with pedv n. however, the ... | 2020 | 31756532 |
| development of a multiplex rt-pcr for the detection of major diarrhoeal viruses in pig herds in china. | the major enteric rna viruses in pigs include porcine epidemic diarrhoea virus (pedv), transmissible gastroenteritis virus (tgev), porcine rotavirus a (prv-a), porcine kobuvirus (pkv), porcine sapovirus (psav) and porcine deltacoronavirus (pdcov). for differential diagnosis, a multiplex rt-pcr method was established on the basis of the n genes of tgev, pedv and pdcov, the vp7 gene of prv-a, and the polyprotein genes of pkv and psav. this multiplex rt-pcr could specifically detect tgev, pedv, pdc ... | 2020 | 31597013 |
| surface-displayed porcine ifn-λ3 in lactobacillus plantarum inhibits porcine enteric coronavirus infection of porcine intestinal epithelial cells. | interferon (ifn)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. lactobacillus plantarum (l. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. alphacoronaviruses, especially porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev), cause high morbidity and mortality in piglets resulting in economic loss. co-infection by these two viruses is becoming increasingly ... | 2020 | 31838830 |
| dynamics of transmissible gastroenteritis virus internalization unraveled by single-virus tracking in live cells. | transmissible gastroenteritis virus (tgev) is a swine enteropathogenic coronavirus that causes significant economic losses in swine industry. current studies on tgev internalization mainly focus on viral receptors, but the internalization mechanism is still unclear. in this study, we used single-virus tracking to obtain the detailed insights into the dynamic events of the tgev internalization and depict the whole sequential process. we observed that tgevs could be internalized through clathrin- ... | 2020 | 32017270 |
| transmissible gastroenteritis virus targets paneth cells to inhibit the self-renewal and differentiation of lgr5 intestinal stem cells via notch signaling. | infection with transmissible gastroenteritis virus (tgev) has been associated with villous atrophy within 48 h, which seriously disrupts intestinal homeostasis. however, the underlying mechanisms remain elusive. in this study, we found that tgev infection severely disrupted intestinal homeostasis via inhibition of self-renewal and differentiation in lgr5 intestinal stem cells (iscs). profoundly, tgev-encoded nsp10/nsp16 protein complex-mediated the inactivation of notch signaling provided a mech ... | 2020 | 31959773 |
| novel sars-cov-2/covid-19: origin, pathogenesis, genes and genetic variations, immune responses and phylogenetic analysis. | in this review, we focused on the origins of the novel coronavirus (sars-cov-2), origin, pathogenesis, immune responses, genes and genetic variations, phylogenetic analyses, and potential therapeutic strategies to summarize approaches for developing broadly effective preventions and vaccines to cope covid-19. towards the end of 2019, sars-cov-2 has emerged in association with the sars, later was named covid-19 caused an environment of chaos worldwide and infected a massive number of lives. since ... | 2020 | 32566803 |
| inhibition of transmissible gastroenteritis coronavirus (tgev) multiplication in vitro by non-immune lymphocytes. | in vitro studies were undertaken to examine the effects of non-immune porcine peripheral blood leukocytes (pbl) on a coronavirus infection due to transmissible gastroenteritis virus (tgev). the assay consisted of tgev-infected epithelial cells expressing viral antigens on the cell surface and producing low amounts of interferon (ifn). non-immune pbl were found to limit virus replication at an effector-to-target ratio of 100/1 even when effector cells were depleted of phagocytic cells. neutralizi ... | 2020 | 32288183 |
| production of monoclonal antibodies targeting aminopeptidase n in the porcine intestinal mucosal epithelium. | porcine aminopeptidase n (apn), a membrane-bound metallopeptidase abundantly present in small intestinal mucosa, can initiate a mucosal immune response without any interference such as low protein expression, enzyme inactivity, or structural changes. this makes apn an attractive candidate in the development of vaccines that selectively target the mucosal epithelium. previous studies have shown that apn is a receptor protein for both enterotoxigenic escherichia coli (e. coli) f4 and transmissible ... | 2021 | 34096912 |
| sars-cov-2 in environmental perspective: occurrence, persistence, surveillance, inactivation and challenges. | the unprecedented global spread of the severe acute respiratory syndrome (sars) caused by sars-cov-2 is depicting the distressing pandemic consequence on human health, economy as well as ecosystem services. so far novel coronavirus (cov) outbreaks were associated with sars-cov-2 (2019), middle east respiratory syndrome coronavirus (mers-cov, 2012), and sars-cov-1 (2003) events. cov relates to the enveloped family of betacoronavirus (βcov) with positive-sense single-stranded rna (+ssrna). knowing ... | 2021 | 32901196 |