Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the effect of octin on baby pigs infected with transmissible gastroenteritis virus. | 1956 | 13362775 | |
| propagation of transmissible gastroenteritis virus in tissue culture. | 1956 | 13395256 | |
| electron microscopy and ether sensitivity of transmissible gastroenteritis virus of swine. | 1965 | 14316771 | |
| experimental transmission of transmissible gastroenteritis virus by starlings. | 1965 | 4957296 | |
| growth of transmissible gastroenteritis virus in young pigs. | 1966 | 4958048 | |
| stained monolayer test: a color test in disposable plastic trays for titrating transmissible gastroenteritis virus and neutralizing antibodies. | 1968 | 4969273 | |
| electron microscopy of tissue culture cells infected with swine transmissible gastroenteritis virus. | 1968 | 4976638 | |
| structure of swine transmissible gastroenteritis virus examined by negative staining. | 1968 | 4182534 | |
| morphologic characteristics and nucleic acid type of transmissible gastroenteritis virus of pigs. | 1968 | 4300586 | |
| the effect of 5-bromodoxyuridine and actinomycin d on the multiplication of transmissible gastroenteritis virus. | 1968 | 4301636 | |
| immunofluorescent detection of transmissible gastroenteritis virus in the intestines of piglets. | 1969 | 4319710 | |
| pathogenicity, immunogenicity and distribution of transmissible gastroenteritis virus in pigs. | 1969 | 4983669 | |
| lesions of the gastrointestinal tract of pigs infected with transmissible gastroenteritis. | gross, subgross and histological lesions were studied in 103 pigs infected with transmissible gastroenteritis virus and killed at daily intervals for 14 days. twenty-three pigs served as controls. thirty-six pigs were given colchicine four hours prior to being killed in order to determine the mitotic activity in the gastrointestinal tract. the gross lesions consisted of dehydration, excessive milk curd in the stomach, focal hemorrhage in the submucosa of the diverticulum ventriculi of the stomac ... | 1969 | 4237292 |
| experimental immunisation of sows against transmissible gastroenteritis. i. immunisation with a bacteria-free suspension of transmissible gastroenteritis virus prepared from the intestines of infected pigs. | 1970 | 4990539 | |
| cell culture-adapted sh strain of transmissible gastroenteritis virus of pigs: in vivo and in vitro studies. | 1970 | 4191129 | |
| morphology of transmissible gastroenteritis virus of pigs. a possible member of coronaviruses. brief report. | 1970 | 4195092 | |
| micro-color test for assay of transmissible gastroenteritis virus-neutralizing antibodies. | 1971 | 4996684 | |
| fluorescent antibody technique in the study of three porcine viruses. transmissible gastroenteritis virus, vomiting and wasting disease virus and the parvovirus 59e-63. | 1971 | 4933149 | |
| distribution of antibodies to transmissible gastroenteritis virus in serum and milk of sows: isolation and identification of the immunoglobulin classes of swine serum and milk. | 1972 | 4623392 | |
| antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. | the antibody response of pregnant swine to transmissible gastroenteritis (tge) virus was studied, with special reference to the titers and the immunoglobulin (ig) class of tge neutralizing antibodies in colostrum and milk. animals vaccinated twice intramuscularly or intramammarily with live attenuated tge virus developed high levels of antibodies in serum and colostrum, but the levels in milk declined markedly within a few days post-farrowing. in contrast, animals naturally or experimentally inf ... | 1972 | 4629259 |
| ribonucleic acid synthesis in porcine cell cultures infected with transmissible gastroenteritis virus. | 1973 | 4631095 | |
| elevation of the intracellular levels of cyclic adenosine monophosphate during in vitro infection of transmissible gastroenteritis virus. brief report. | 1973 | 4353800 | |
| porcine coronaviruses: co-infection of cell cultures with transmissible gastroenteritis virus and hemagglutinating encephalomyelitis virus. | 1973 | 4196485 | |
| electron microscopy of intestinal epithelial cells of piglets infected with a transmissible gastroenteritis virus. | an electron microscopic study of intestinal epithelial cells of neonatal piglets infected with transmissible gastroenteritis (tge) virus revealed a unique parasite-host cell interaction. entry of the tge virus into intestinal epithelial cells of newborn piglets is mediated through a network of cytoplasmic tubules of plasmalemma origin. the tubules, called microcanaliculi, are morphologically distinct from endoplasmic reticulum and golgi. in uninfected animals similar tubules appear to be respons ... | 1973 | 4266698 |
| secretory antibodies in milk of swine against transmissible gastroenteritis virus. | 1974 | 4607052 | |
| isolation of transmissible gastroenteritis virus from lungs of market-weight swine. | 1974 | 4607634 | |
| transmissible gastroenteritis virus in neonatal pigs: intestinal transfer of colostral immunoglobulins containing specific antibodies. | 1974 | 4819717 | |
| upper respiratory infection of lactating sows with transmissible gastroenteritis virus following contact exposure to infected piglets. | ten breeding sows were left in direct contact with their newborn piglets that had been experimentally infected with transmissible gastroenteritis (tge) virus. all sows became infected with the virus. the sows developed fever and showed mild clinical signs of the disease for a few days. the sows excreted virus in the nasal secretion, feces, and milk during the acute febrile phase of illness. virus was isolated from the nasal secretion of one sow as early as 20 hours after contact exposure to the ... | 1975 | 166796 |
| effect of precipitation with methanol on antigenic potency of tge virus. | 1975 | 167507 | |
| the demonstration of transmissible gastroenteritis viral antigens by immunoelectrophoresis and counterimmunoelectrophoresis. | immunoelectrophoresis of alkaline intestinal extracts, or the supernatants after precipitation of these extracts with 60% ammonium sulfate, prepared from piglets experimentally infected with the dl or purdue strains of transmissible gastroenteritis virus, revealed up to three antigens. two antigens migrated towards the anode, and the third migrated towards the cathode. antigens with anodal or cathodal mobility were also demonstrated in the same materials by counterimmunoelectrophoresis, and the ... | 1975 | 167926 |
| viral susceptibility of a cell line derived from the pig oviduct. | seventeen of 24 rna viruses and eight of nine dna viruses replicated in a cell line derived from a pig fallopian tube. the following rna viruses grew poorly in it: the virus of transmissible gastroenteritis of pig and the swine-influenza, sendai and bovine para-influenza type 3 viruses. among other rna viruses an untyped swine para-myxovirus and some picornaviruses, rhabdoviruses and togaviruses attained high titers and produced an extensive cytopathic effect. among the dna viruses a porcine ade ... | 1975 | 169971 |
| transmissible gastroenteritis (tge) of swine: effect of age of swine testes cells culture monolayers on plaque assays of tge virus. | a continuous line of swine testes cell culture monolayers was infected at various ages with both cell culture-adapted transmissible gastroenteritis (tge) virus and tissue infected with tge virus. both produced increasing numbers of plaques as the cell monolayers aged from two to five days. therefore, allowing the swine testes cell monolayer to age five to six days before inoculation should increase the likelihood of detecting tge virus by plaque assay. | 1975 | 169972 |
| identification of heat-dissociable rna complexes in two porcine coronaviruses. | 1975 | 170536 | |
| the polypeptide structure of transmissible gastroenteritis virus. | the polypeptides of purified preparations of the coronavirus responsible for transmissible gastroenteritis of pigs have beem examined by polyacrylamide gel electrophoresis. four major polypeptides, vpi (mol. wt. 200000), vp2 (50 000), vp3 (30000) and vp4 (28500) and two minor polypeptides, vpia (105000) and vpib (80500) have been reproducibly demonstrated in the virion, of which vpi, vp3 and vp4 contain carbohydrate. treatment of the virion with the proteolytic enzyme bromelain removes the surfa ... | 1975 | 171335 |
| vaccination experiments against transmissible gastroenteritis (tge) of swine. iii. the virus-neutralizing titre of vaccinated sows' milk and its persistence during the lactation period. | 1975 | 171935 | |
| vaccination experiments against transmissible gastroenteritis (tge) of swine. iv. the titre and persistence of virus-neutralizing antibodies in the sera of sows vaccinated once or twice at different intervals. | 1975 | 171936 | |
| [use of the direct immunofluorescence method for determination of the tge viral antigen in swine]. | followed up were the appearance and accumulation of the transmissive gastroenteritis (tge) virus antigen in infected cells of pig kidney and thyroid. used were specific conjugates, such introduced by the authors and some imported from czechoslovakia. the cell cultures were infected with strains of the tge virus, isolated in bulgaria or supplied from czechoslovakia. it was found that the bulgarian strains were the same as those from czechoslovakia and the united states. a viral antigen was found ... | 1975 | 174279 |
| [viral gastroenteritis in swine]. | 1975 | 175547 | |
| the influence of ph on the growth and stability of transmissible gastroenteritis virus in vitro. | the influence of ph on the growth of transmissible gastroenteritis virus (tgev) in adult pig thyroid cell culture, and on the stability of the virus was studied. at ph 7.2 and 100 fold higher than those at ph 8.0. the adsorption, penetration and uncoating steps of the viral replicative cycle were shown to be unaffected by ph variation. synthesis of tgev rna during the first 12 hours post infection was found to be unaffected by ph variation between the range 6.5-8.0. after 12 hours breakdown of t ... | 1975 | 2142 |
| classifying gastroenteritis viruses. | 1975 | 78165 | |
| comparison of properties between virulent and attenuated strains of transmissible gastroenteritis virus. | strains of transmissible gastroenteritis (tge) virus possessing different pathogenicity were examined for stability to digestive enzymes and acid, and growth at various temperatures. in growth experiments, virus titer obtained at 37 degrees c were about equal between attenuated and virulent strains, but titers attained by the attenuated strain were higher at 30 degrees c. the attenuated virus multiplied at 28 degrees c, but the virulent virus did not at this temperature. the virulent virus was s ... | 1975 | 1684 |
| protective effect of immunoglobulins in serum and milk of sows exposed to transmissible gastroenteritis virus. | experimental exposure of susceptible pregnant sows by various routes to the gut-origin transmissible gastroenteritis virus stimulated production of milk and serum antibodies. these antibodies neutralized the cytopathic effect of transmissible gastroenteritis virus propagated in cell culture. this in vitro neutralizing antibody resided in the igg and iga immunoglobulin classes. on the other hand, protection for baby pigs resided in the iga class of milk immunoglobulin of sows exposed orally or in ... | 1975 | 1111857 |
| protein loss in piglets infected with transmissible gastroenteritis virus. | 1975 | 1130146 | |
| [some properties of strains of the transmissible gastroenteritis virus of swine (tge) isolated in bulgaria]. | strains of the swine transmissive gastroenteritis (tge) virus were isolated for the first time in bulgaria in 1972. the dynamics was followed up of some strains' propagation in primary cell cultures of kidney tissue and subcultures of the thyroid of pigs. the intracellular virus reached highest titers at the 24--48th hour in the kidney cells, and at the 24--36th hour in the thyroid cells, while the extracellular virus was subjected to inactivation by the 2nd hour after infecting the cultures, by ... | 1975 | 175555 |
| recovery of transmissible gastroenteritis virus from chronically infected experimental pigs. | transmissible gastroenteritis (tge) virus was reisolated from pulmonary and intestinal tissues from 6 of 9 chronically infected experimental pigs (principals) necropsied 30 to 104 days after inoculation. tissue homogenates (lung and small intestine) from the principals were prepared and inoculated into 3- to 5-day-old gnotobiotic pigs. the virus reisolated from the tissue homogenates produced a milder disease on 1st passage and a more severe disease on 2nd passage. the chronically infected exper ... | 1975 | 171980 |
| [isolation of the virus of transmissible gastroenteritis of swine from cell cultures and antigenic comparison of 2 american strains]. | 1975 | 164273 | |
| age-dependent resistance to transmissible gastroenteritis of swine. iii. effects of epithelial cell kinetics on coronavirus production and on atrophy of intestinal villi. | coronavirus titers in small intestine, degree of villous atrophy and apparent rates of regeneration of intestinal villi were compared in newborn, 3-week-old and adult pigs for 1 week after they were exposed to the transmissible gastroenteritis virus of swine. the response within the newborn group was homogeneous, resulting in high virus titers, maximal villous atrophy and comparatively slow regeneration. in general, virus titers were lower, villous atrophy was less severe and regeneration more r ... | 1975 | 1229058 |
| transmissible gastroenteritis of swine. efficacy assay of an intramammary use vaccine. | tge seronegative pregnant gilts were vaccinated by intramammary inoculations with a chemically inactivated (betapropiolactone) cell culture virus vaccine and their nursing pigs were exposed to virus at three days of age. the pig morbidity and survival rates were compared with those of pigs nursing gilts exposed orally to virulent tge virus during late gestation and also with pigs nursing non-vaccinated seronegative sows. the morbidity rates were 100%, 19% and 19%, while the survival rates were ... | 1976 | 182600 |
| pathogenicity of an attenuated strain of transmissible gastroenteritis virus for newborn pigs. | the pathogenicity of a cell culture-attenuated strain of transmissible gastroenteritis virus for newborn pigs was investigated. newborn (1- to 2-day-old) pigs were orally given 2 x 10(6) plaque-forming units of attenuated virus. all pigs developed mild diarrhea, but deaths did not occur. as determined by immunofluorescence and villous atropy, infection of the small intestine was limited to the caudal 50 to 66%. fluorescing cells and atrophic villi were seen from 2 to 3 days until 6 to 7 days aft ... | 1976 | 176906 |
| transmissible gastroenteritis virus: plaques and a plaque neutralization test. | a plaquing system and plaque neutralization test in porcine thyroid cells were used to study different transmissible gastroenteritis isolates and hemagglutinating encephalomyelitis virus. among transmissible gastroenteritis virus isolates, plaque size varied considerably and mixed size ranges sometimes occurred. the most recently isolated viruses produced smaller plaques than the laboratory viruses or hemagglutinating encephalomyelitis virus. all transmissible gastroenteritis virus isolates reac ... | 1976 | 187296 |
| the detection of transmissible gastroenteritis viral antibodies by immunodiffusion. | precipitating antibodies against transmissible gastroenteritis viral antigens were detected by the immunodiffusion test in two transmissible gastroenteritis viral hyperimmune antisera and in antiserum prepared against haemagglutinating encephalomyelitis virus but not in sera from several species of normal animals, in antisera prepared against a variety of othet viruses and bacteria or sera from swine with bacterial enteritis. when the immunodiffusion test was compared with the virus neutralizati ... | 1976 | 187295 |
| leukocyte-aggregation assay for transmissible gastroenteritis of swine. | an in vitro leukocyte-aggregation assay was developed to detect the exposure of swine to transmissible gastroenteritis virus. leukocytes in heparinized blood samples aggregated when mixed with test antigen prepared from transmissible gastroenteritis-infected swine testicle cell cultures. twenty-two of 23 swine exposed 3 days or more were positive or suspects in the assay; 6 nonexposed swine were negative. aggregation was shown as early as 3 days postexposure in 1 sow and persisted for as long as ... | 1976 | 187089 |
| vaccination of newborn pigs with an attenuated strain of transmissible gastroenteritis virus. | clinical signs of transmissible gastroenteritis were not observed in newborn pigs orally inoculated with the high-passaged vaccinal transmissible gastroenteritis virus (to-163 strain). vaccinal viral multiplication in digestive tract of newborn pigs fed colostrum before inoculation and kept at 21 to 22 c was diminished, but was not diminished in those fed colostrum and kept at 10 to 11 c. other groups of newborn pigs inoculated with the attenuated vaccinal virus and kept at 18 to 22 c or at 31 t ... | 1976 | 187088 |
| viral gastroenteritis: recent progress, remaining problems. | infants and young children are particularly susceptible to a recently identified viral enteritis which is highly contagious and seems both common and universal. in this disease, virus invades the upper intestinal epithelium, causing acute diarrhoea with early fever and vomiting. we studied a similar disease in pigs, infecting three-week-old animals with transmissible gastroenteritis virus (tge), which also invades the upper intestinal epithelium. in this model, diarrhoea is massive 16-40 hours a ... | 1976 | 1049555 |
| in vitro differentiation and ph sensitivity of field and cell culture-attentuated strains of transmissible gastroenteritis virus. | characteristics of four transmissible gastroenteritis (tge) virus field strains (miller, purdue, bl, and v203) and four cell culture-attenuated strains (purdue, sh, ckp, and bl) were studied to find methods of differentiation between the two groups of viruses. tge field virus strains did not replicate as well as attenuated strains at 37 c and could not be passaged serially for more than four to six passages at 33 c. there were clear differences in plaque size when the strains were compared. fiel ... | 1976 | 9360 |
| isolation of subviral components from transmissible gastroenteritis virus. | exposure of purified transmissible gastroenteritis virus, a porcine coronavirus, to non-ionic detergents resulted in the removal of the surface projections and greater than 98% of the virus lipid. virus rna was associated with a subviral particle which had a sedimentation coefficient of 650s, compared with 495s for the intact virion, and which banded in cs2so4 gradients at 1-295 g/ml. negatively stained preparations of subviral particles were shown by electron microscopy to contain spherical par ... | 1976 | 190341 |
| intestinal immune response of feeder pigs to infection with transmissible gastroenteritis virus. | five feeder pigs 4 to 6 months old were orally inoculated with transmissible gastroenteritis virus. diagnosis of transmissible gastroenteritis was made on the basis of clinical signs and examination of intestinal mucosa by the fluorescent antibody technique. immunoglobulins were extracted from intestinal fluid of infected feeder pigs. virus-binding and neutralizing antibodies were detected in intestinal extracts between 7 and 56 days after infection. the concentration of binding antibodies reach ... | 1976 | 944001 |
| properties of the "ckp" transmissible gastroenteritis virus strain. i. immunofluorescence assay on the viral replication in cell cultures. | 1976 | 205121 | |
| [epizootiology of viral (transmissible) gastroenteritis of swine]. | 1976 | 176769 | |
| partial characterization of the principal soluble antigens associated with the coronavirus of transmissible gastroenteritis by complement fixation and immunodiffusion. | a microtiter complement fixation (cf) test to detect transmissible gastroenteritis (tge) viral antigen was developed, using tge hyperimmune pig serum as an antibody source. sera from tge covalescent pigs did not fix complement by this test. maximal virus and soluble antigen (sa) titers were obtained 36 to 48 h after inoculation of swine testes cells. cell-associated virus and sa titers were higher than those in the culture fluid, which had to be concentrated 20x before use as antigen in agar imm ... | 1976 | 177369 |
| effect of ambient temperatures on multiplication of attenuated transmissible gastroenteritis virus in the bodies of newborn piglets. | newborn piglets were found to be more resistant to infection with attenuated transmissible gastroenteritis virus when maintained at higher temperatures. this was attributed to a decreased rate of virus propagation and spreading in the bodies of the infected animals. the highest virus levels were detected in the tissues of piglets maintained at 8 to 12 c. in contrast, no virus was recovered from piglets maintained at 35 to 37.5 c. the virus was found only in the lymph nodes and respiratory organs ... | 1976 | 178601 |
| chromatographic separation of gram quantities of immunoglobulins from porcine colostrium against transmissible gastroenteritis virus. | similar immunoglobulin (ig) classes were obtained from porcine colodtral whey by either column or batch chromatographic procedures; a stepwise buffer elution technique was used. specific transmissible gastroenteritis virus neutralizing antibody was found in the 4 major fractions eluted comprising of igg1, igg2, iga, and igm. the igg1, and igg2 were essentially homogeneous, and the iga- and igm-rich fractions had to be recycled several times through sephadex g-200 to obtain pure iga and igm that ... | 1976 | 180183 |
| evaluation of 6-azauridine and 5-iododeoxyuridine in the treatment of experimental viral infections. | the potential antiviral activity of 6-azauridine and 5-iododeoxyuridine was evaluated in a coordinated study at five institutions. experimental models in five species, the mouse, rabbit, swine, cat, and ferret, were established with use of 10 viruses: herpesvirus hominis types 1 and 2, murine cytomegalovirus, vaccinia virus, shope fibroma virus, transmissible gastroenteritis virus, swine influenza virus, feline viral rhinotracheitis virus, feline panleukopenia virus, and ferret distemper virus. ... | 1976 | 180189 |
| the isolation of reovirus-like agents (rota-viruses) from acute gastroenteritis of piglets. | isolations of reovirus-like agents (rotaviruses) were made from nine of 23 outbreaks of piglet diarrhoea on different farms and from both weaned and unweaned piglets. the viruses were shown to be morphologically and anti-genically similar to the rotaviruses of children and calves. gnotobiotig piglets given intranasal inoculations of five different isolates developed acute gastroenteritis, and the virus was re-isolated from the faeces or intestinal contents. the piglet virus was not adapted to re ... | 1976 | 180294 |
| neutralization of a transmissible gastroenteritis virus of swine by colostral antibodies elicited by intestine and cell culture-propagated virus. | cross-protection studies of gilts exposed to 4 transmissible gastroenteritis viruses--ilinois (field strain), miller-3, miller low passage (m-lp), and miller high passage (m-hp) tissue culture-adapted--indicated that only the gilt vaccinated with illinois strain was protected, along with its newborn pigs, against challenge exposure with field virus. similar results were obtained when the 4 viruses were incubated in vitro with colostrum from each of the 4 vaccinated gilts and subsequently used to ... | 1976 | 183573 |
| antibody response in pigs inoculated with transmissible gastroenteritis virus and cross reactions among ten isolates. | groups of two or three day old pigs were inoculated intravenously with cell culture grown transmissible gastroenteritis virus. a single or a multiple dosage schedule was used. the magnitude of immune response was measured in terms of serum neutralization indices. a single dose of relatively attenuated virus caused mild clinical signs of transmissible gastroenteritis infection in the pigs and induced a low level of antibody in the serum by the seventh day after inoculation. repeated injections of ... | 1976 | 187297 |
| [transmissible gastroenteritis in swine (author's transl)]. | transmissible gastroenteritis or tge is a virus diarrhoea which occurs in pigs of all ages and is associated with high mortality rates in the young piglets. growth of virus in the columnar epithelium of the small intestine causes atrophy of the intestinal villi, malabsorption, watery diarrhoea and dehydration. faecal excretion of virus usually continues up to fourteen days after infection but chronic carriers have been found to occur. tge is self-limiting on the majority of pig-breeding farms bu ... | 1976 | 175523 |
| vaccination experiments against transmissible gastroenteritis (tge) of swine. v. relationship between the titre and the antigenic effect of the vaccine. | 1976 | 200132 | |
| detection of transmissible gastroenteritis virus neutralising antibody in cats. | high titres of neutralizing activity to transmissible gastroenteritis virus (tgev), a porcine coronavirus, were found in sera and peritoneal fluids from cats infected with feline infectious peritonitis (fip). a small proportion of cats, from a hospital population unaffected by fip, also had neutralising activity. procedures to remove non-specific viral inhibitors, including treatment by heat inactivation, trypsin, sulphydryl reagent and kaolin absorption were unsuccessful. the active component w ... | 1977 | 200203 |
| transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation. | isolation of transmissible gastroenteritis virus was attempted from segments of jejunum collected from piglets submitted for diagnosis of transmissible gastroenteritis. the virus was isolated more frequently in susceptible piglets than in pig kidney or pig thyroid cells. practically, both cell systems were equally capable of demonstrating the virus when the tissue suspensions were sonicated. the pig thyroid cells prepared with glands collected from minimal disease pigs were preferred to the pig ... | 1977 | 200316 |
| comparison of intestinal (illinois strain) and cell culture-adapted (m-hp strain) viral populations of transmissible gastroenteritis of swine. | intestinal and cell culture-adapted viral populations of transmissible gastroenteritis (tge) of swine were compared by means of sucrose gradient centrifugation, immunnofluorescence, electron microscopy, immune electron microscopy, statistical analysis of the number of plaque-forming units, and ultraviolet sensitivity. results indicated that the size range and general coronavirus morphologic characteristics were shared by both viral populations. marked morphologic variations existed among particl ... | 1977 | 201184 |
| cell culture propagation of porcine rotavirus (reovirus-like agent). | two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ... | 1977 | 201198 |
| [diagnosis of transmissible gastroenteritis of swine]. | 1977 | 202057 | |
| [test of an attenuated viral strain of tge as a vaccine]. | a live vaccine was produced using a local strain p of the virus of the transmissive gastroenteritis, arrenuated in cell cultures. the vaccinated pregnant sows had high-titer serum and colostrum virus-neutralizing antibodies. in the first days following farrowing there were in the colostrum ummunoglobulins of the igg class that prevailed, however, iga and igm proved more effective in the virus-neutralizing test. newborn pigs acquired passive immunity at about the 24th hour after the intake of col ... | 1977 | 202064 |
| immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. | immune electron microscopy (iem) was developed as a diagnostic aid for detecting and identifying transmissible gastroenteritis virus and rotavirus (reovirus-like agent) in fecal and intestinal contents from cases of gastroenteritis in young pigs. variables involved in use of direct iem and its sensitivity were determined. aggregates of virus coated with specific antibody were seen in virus samples mixed with homologous convalescent antiserum, but not in control samples containing preexposure ser ... | 1977 | 189646 |
| [attempts to establish an immunoprevention of transmissible gastroenteritis (tge) in swine. i. pathogenicity of the b1 strain following serial passages]. | 1977 | 202115 | |
| micro-indirect hemagglutination test for detection of antibody against transmissible gastroenteritis virus of pigs. | a micro-indirect hemagglutination (iha) test was developed for detecting antibody against transmissible gastroenteritis (tge) virus of pigs. tge virus propagated in swine kidney cell cultures was highly purified and concentrated by the combination of ammonium sulfate precipitation, treatment with fluorocarbon, and sucrose density gradient centrifugation. tanned sheep erythrocytes were sensitized with purified virus for use in the iha test. the results of testing 104 serum samples collected from ... | 1977 | 197119 |
| lipids of transmissible gastroenteritis virus and their relation to those of two different host cells. | the lipids of two cell types (primary pig kidney and secondary adult pig thyroid) and those of transmissible gastroenteritis virus (tgev) grown in these cells were studied using 14c-palmitic acid. differences were demonstrated between the incorporation of isotopically labelled lipid precursors in the two cell types and it was found that the phospholipid and glycolipid profiles of purified tgev closely resembled those of the host cell in which it was grown. | 1977 | 192838 |
| further studies on human enteric coronaviruses. | comparisons were made between human enteric coronaviruses and the enteric coronaviruses of pigs and calves by negative staining. examination of human intestinal organ culture fluids at various time intervals after inoculation with the human enteric coronavirus showed increasing numbers of particles in the fluids. thin sections of the columnar epithelial cells of these explants showed a number of features consistent with the replication of known human and animal coronaviruses. virus particles fou ... | 1977 | 196571 |
| immunoglobulin classes of antibodies in milk of swine after intranasal exposure to pseudorabies virus or transmissible gastroenteritis virus. | experiments were conducted to evaluate whether infection of the respiratory tract of pregnant swine with pseudorabies (pr) virus would induce the secretion of immunoglobulin a (iga) antibodies in their milk as was observed after enteric infection with transmissible gastroenteritis (tge) virus. the immune response of sows to pr virus inoculated intranasally and to tge virus inoculated orally/intranasally or via a natural infection was studied. emphasis was placed upon titers and ig classes of pr ... | 1977 | 197014 |
| [problems in the specific prophylaxis of viral gastroenteritis in swine]. | 1977 | 197381 | |
| leukocyte migration-inhibition procedure for transmissible gastroenteritis viral antigens. | swine exposed to transmissible gastroenteritis viral antigens developed humoral and cell-mediated immunity. migration of leukocytes from exposed swine was inhibited in the presence of the sensitizing antigens, whereas migration of leukocytes from nonexposed swine was not inhibited in the presense of these same antigens. in virus-neutralization-positive animals, it was not possible to correlate degree of inhibition with virus-neutralization titer. inhibition was observed 7 days after exposure and ... | 1977 | 199095 |
| seroepidemiology of feline infectious peritonitis virus infections using transmissible gastroenteritis virus as antigen. | 1977 | 341605 | |
| transmissible gastroenteritis: sodium transport and the intestinal epithelium during the course of viral enteritis. | sodium transport, mucosal structure, and epithelial enzymes were studied in piglets killed 10, 25, 40, 72, or 144 hr after infection with a standard dose of transmissible gastroenteritis virus. glucose-stimulated na transport measured in short-circuited jejunal epithelium and suspensions of villous enterocytes became progressively more abnormal during the first 40 hr, but recovered completely by 144 hr. as na transport deteriorated, jejunal mucosal villi shortened and crypts deepened; cells isol ... | 1977 | 832794 |
| transmissible gastro-enteritis of swine in the netherlands the application of the direct fluorescent antibody techique for diagnosis. | the epizootiology of transmissible gastro-enteritis (tge) of swine in 1975 and 1976 in the netherlands was studied. diagnosis was made by the direct fluorescent antibody technique (fat). specimens from the small intestine of suspected animals were examined for this purpose. the results obtained by the direct fat were proved to be reliable, if the specimens were derived from acutely sick animals and examination could be carried out as soon as possible. the results confirmed that tge occured in an ... | 1977 | 196358 |
| quantitative transmissible gastroenteritis virus shedding patterns in lactating sows. | to test the role of sows in spreading transmissible gastroenteritis (tge), 11 sows were intravenously, intranasally, or intramammarily inoculated with virulent virus within 5 days of farrowing. six of the sows were separated from their offspring, and 5 were allowed to nurse their litters. all sows became clinically ill with sign of anorexia, depression, and fever that persisted until postinoculation day 4 or 5. they shed virus through milk, nasal secretions, and feces, with individual variations ... | 1977 | 192108 |
| antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species. | utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ... | 1978 | 81044 |
| a new coronavirus-like particle associated with diarrhea in swine. | coronavirus-like particles were detected by electron microscopy in the intestinal contents of pigs during a diarrhea outbreak on 4 swine breeding farms. diarrhea was reproduced in experimental pigs with one of the isolates, designated cv777, which was found to be distinct from the 2 known porcine coronaviruses, transmissible gastroenteritis virus and hemagglutinating encephalomyelitis virus. | 1978 | 83132 |
| [resistance and inactivity of transmissible gastroenteritis virus of swine (tge)]. | 1978 | 212010 | |
| colostral iga, igg, and igm-iga fractions as fluorescent antibody for the detection of the coronavirus of transmissible gastroenteritis. | colostrum from sows and gilts inoculated with virulent transmissible gastroenteritis virus was fractionated into the 3 major immunoglobulin classes, iga, igg, and igm-iga fractions, by chromatographic and gel-filtration procedures. each fraction was assayed for purity with rabbit anti-porcine serum and rabbit monospecific anti-porcine igg, anti-porcine iga, and anti-porcine igm. these analyses showed that the igg and iga fractions were pure. the igm fraction contained some iga in the polymeric f ... | 1978 | 211888 |
| the postulated role of feeder swine in the perpetuation of the transmissible gastroenteritis virus. | clinical, immunofluorescence and histopathological observations were found to be an efficient approach for the confirmation of the diagnosis of transmissible gastroenteritis in feeder swine. two cases are reported to exemplify how feeder swine exposed to points of concentration such as holding areas, sales barns and auctions can play an important role in the epizootiology of transmissible gastroenteritis. a third field case is reported as an example of an outbreak of transmissible gastroenteriti ... | 1978 | 217504 |
| field trials on transmissible gastroenteritis live virus vaccine in newborn piglets. | field trials were conducted on attenuated live virus vaccine of transmissible gastroenteritis to confer active immunity to newborn piglets. to examine innocuity and efficacy of the vaccine, a total of 714 newborn piglets were subjected to these trials. of them, 357 piglets were administered orally with 10(7.0) tcid50 within 3 days after birth, and the other 357 piglets served as nonvaccinated controls. no undesirable postvaccinal reaction was observed in any vaccinated piglet. suckling piglets b ... | 1978 | 216927 |
| the use of immunofluorescence techniques for the laboratory diagnosis of transmissible gastroenteritis of swine. | over a four year period, 74 of 250 field outbreaks of enteric disease (30%) and 110 of 440 swine (25%) were positive for transmissible gastroenteritis by immunofluorescence procedures. of 141 swine from herds positive for transmissible gastroenteritis 110 (78%) were positive by fluorescent antibody techniques. the fastest, easiest to perform and most effective procedure was the examination of frozen sections of the jejunum from acutely ill animals by the fluorescent antibody tissue section techn ... | 1978 | 217505 |
| rotavirus as a cause of diarrhea in pigs. | a rotavirus (reovirus-like agent) was associated with diarrheal diseases occurring in 1- to 4-week-old suckling pigs in 8 herds and in weaned pigs in 2 herds. transmissible gastroenteritis virus was also detected in 2 of these herds, as was enteropathogenic escherichia coli in 5 herds. morbidity was generally greater than 80% in pigs of the affected age group within these herds, and mortality from diarrhea ranged from 7 to 20%. the disease due to rotavirus in suckling pigs appeared similar to th ... | 1978 | 203565 |
| isolation of transmissible gastroenteritis virus from pharyngeal swabs obtained from sows at slaughter. | a virologic survey was conducted to determine the frequency of transmissible gastroenteritis (tge) virus infection in farm-raised sows. pharyngeal swab specimens collected in an abattoir were examined for tge virus by inoculation onto swine-testes cell culures. the virus was detected in 61 (3%) of a sample of 2,058 iowa sows after slaughter. all tge viral isolates, given orally to 2- or 3-day-old pigs, caused acute gastroenteritis and in some cases death. all pigs that recovered from illness had ... | 1978 | 206173 |
| [attempts to develop an immunoprevention for transmissible gastroenteritis (tge) in swine. ii. immunogenicity of the b1 strain following serial passages]. | 1978 | 207054 | |
| intrafetal inoculation of swine with transmissible gastroenteritis virus. | fetuses in 3 sows were inoculated (intramuscularly) with transmissible gastroenteritis (tge) virus on 95th, 77th, and 74th days of the gestation. at 15, 14, and 37 days later (or days when pigs were obtained by hysterectomy), there was evidence of intestinal localization of virus, with villous atrophy and subsequent repair. all intrafetal-inoculated pigs became serologic-positive for tge. a noninoculated pig shown to be seropositive for tge at 15 days of age (after hysterectomy) was resistant to ... | 1978 | 208434 |
| [action of actidion on the replication of the transmissible gastroenteritis virus]. | explored was the possibility of inhibiting the replication of the virus of the transmissive gastroenteritis by means of actidione. it was found that in certain concentrations the antibiotic completely inhibited the replication of this virus. in individual strains the process of replication was not influenced by the various concentrations applied. it was demonstrated that when the antibiotic was added to the infected cell cultures during individual phases its varying concentrations influenced the ... | 1978 | 210563 |
| passive immunization against transmissible gastroenteritis virus in piglets by ingestion of milk of sows inoculated with attenuated virus. | pregnant sows were inoculated with the attenuated strain, to--163, of swine transmissible gastroenteritis virus. suckling piglets born from them received challenge inoculation with the virulent virus at 3 days after birth, and examined for ability to prevent infection and the immunoglobulin (ig) classes of antibody in milk. a pregnant sow was inoculated intramuscularly with a dose of 10(8.0) tcid50 and intranasally with a dose of 10(9.3) tcid50 of attenuated virus. piglets born from it suffered ... | 1978 | 211443 |
| [transmissible gastroenteritis of swine as a model for infectious diarrhea]. | transmissible gastro-enteritis is a virus-caused diarrhoeal disease of swine which may result in up to a total loss among newborn animals. changes are, primarily, of pathologicoanatomic nature and take place in the small intestine. the epithelium of the intestinal villi is completely destroyed, and diarrhoea is the result. deformation in blood composition is a secondary sequel. the diarrhoea proper causes dehydration and acidosis which, in conjunction with abnormal heart function due to hyperkal ... | 1978 | 211981 |