| prevalence of antibodies against hepatitis c virus in primary biliary cirrhosis and autoimmune chronic hepatitis. | we have determined the prevalence of antibodies against hepatitis c virus (anti-hcv) in 45 patients with primary biliary cirrhosis (pbc) and 6 with autoimmune chronic active hepatitis (ai-cah). anti-hcv was positive in two cases of pbc, both with a history of previous blood transfusion, and in one patient with ai-cah, only during an active phase of the disease. | 1992 | 1333324 |
| prevalence of antibody to hepatitis c virus in acute non-a, non-b hepatitis in patients from different epidemiological categories. | the prevalence of antibodies to hepatitis c virus (hcv) was determined in 65 patients with acute non-a, non-b hepatitis (nanbh). the results suggest that hcv is the most common causative agent in posttransfusion nanbh and in drug-related hepatitis. detection of hcv antibodies does not appear to be a particularly useful diagnostic criterion due to the kinetics of the immune response in the course of the disease. | 1992 | 1333327 |
| prevalence of anti-hcv antibodies in patients with acute nona-nonb viral hepatitis. | in a group of 55 patients with nanbh, 81% were found to be reactive for hcv antibodies. in addition, many patients who had not been subject to parenteral risk of infection were also found to be reactive. statistically, hcv positive patients have an increased tendency to develop chronic hepatitis. | 1992 | 1333328 |
| prevalence of antibody to hepatitis c virus in chronic hbsag carriers. | the presence of the anti-hcv antibody was investigated in sera from 102 chronic hbsag carriers. the subjects varied as to the characteristics of the clinical states. it was found that hcv coinfection was more common in hbsag positive intravenous drug addicts than in other parenteral risk groups. it also appears that hcv may be the causative agent of chronic liver disease in hbsag carriers with undetectable hbv (and possibly hdv) replication. | 1992 | 1333329 |
| prevalence of hepatitis c virus (hcv) antibodies in haemodialysis patients. | the prevalence of antibodies to hcv and the course of hepatitis have been determined in 357 haemodialysed patients treated at a single institution. the prevalence of hcv infection increases with the duration of haemodialysis and with the use of blood transfusions, yet there is high frequency of hcv seropositivity even without blood transfusions. evolution of hcv hepatitis to chronicity is frequent and biological signs of chronic hepatopathy can coexist with absence of alanine aminotransferase (a ... | 1992 | 1333330 |
| prevalence of anti-hcv in two tanzanian villages. | the presence of anti-hcv antibodies was investigated in sera from a total of 123 inhabitants of two tanzanian villages. in one of the villages, 72.2% of the sera and in the other village, 82.6% of the sera were found to be anti-hcv positive. these values are dramatically higher than other reported prevalences, whereby cross-reactivity between hcv and flaviviruses as well as possible transmission by arthropod vectors cannot be ruled out. | 1992 | 1333331 |
| storage conditions of blood samples and primer selection affect the yield of cdna polymerase chain reaction products of hepatitis c virus. | we have noticed that suboptimal specimen processing and storage conditions may cause false-negative results in the detection of hepatitis c virus (hcv) rna in plasma or serum. to establish the influence of specimen handling in a serological laboratory on the rate of detection of hcv rna by the cdna polymerase chain reaction (cdna-pcr), we tested routine serum samples and fresh-frozen plasma samples from the same bleeding from confirmed anti-hcv-positive blood donors. when primers from the ns3/ns ... | 1992 | 1333489 |
| [diagnosis and therapy of hepatitis c]. | | 1992 | 1333508 |
| antibody to hiv-1, htlv-i, and hcv in three populations of rural haitians. | the seroprevalence of antibodies to hiv-1, htlv-i, and hcv was evaluated in three populations from northern rural haiti: 1,727 patients attending the hospital for symptoms suggestive of hiv disease, 228 consecutive surgical patients, and 500 pregnant women were tested. hiv-1 seroprevalence was 6.1 and 4.0% in the last two groups, respectively, and 39.3% in the symptomatic population. associated symptoms of wasting, cough, and diarrhea and a clinical diagnosis of aids were significantly predictiv ... | 1992 | 1333530 |
| [occurrence of antibodies against hepatitis c virus (anti-hcv) in patients on long-term hemodialysis]. | the prevalence of anti-hcv antibodies, hbs antigenemia and transaminase level were examined in the 136 patients with chronic renal failure on maintenance hemodialysis, treated in dialytic centers of south poland. in this group anti-hbs and anti-hbc antibodies were also investigated in 60 and 42 patients respectively. anti-hcv antibodies were present in 16.7-60.0% (mean = 44.1%) of patients depending on the center. these values considerably exceed the percentage of antigen hbs positive patients b ... | 1992 | 1333622 |
| non-radioisotopic methods for dna probes. | we have recently developed a new colorimetric method, dna enzyme immunoassay (deia), for detecting specific hybrids of complementary nucleic acids. this technology is based on an antibody that selectively recognizes double, but not single-stranded dna. the molecule does not react with a specific probe immobilized on microwells through an avidin-biotin bridge, nor with non-specific amplified sequences, since they are removed by washing. the antibody reveals the hybridization event, independently ... | 1992 | 1333735 |
| interferon therapy for non-a, non-b hepatitis: a pilot study and review of the literature. | we treated four cases of acute unresolving non-a, non-b hepatitis, and eleven cases of chronic non-a, non-b hepatitis with recombinant interferon alpha-2a for up to one year. the dose of interferon was initially 3 million units daily, and was gradually decreased to 1 million units three times weekly. the overall response rate was 80 percent (twelve out of fifteen cases) at the end of treatment. relapse occurred after the cessation of treatment in seven of the eight cases of chronic disease respo ... | 1992 | 1334037 |
| detection of hepatitis c viraemia in caucasian patients with hepatocellular carcinoma. | potential risk factors for the development of hepatocellular carcinoma were analysed in 40 caucasian patients with this malignancy. a higher proportion (14 of 40; 35%) had evidence of hepatitis c virus (hcv) infection than had evidence of either hepatitis b virus (hbv) carriage (17.5%) or alcohol abuse (30%). in all 14 patients whose sera were reactive by hcv elisa (ortho second generation test), the presence of antibodies to hcv were confirmed by recombinant immunoblot assay (ortho riba-2). fur ... | 1992 | 1334130 |
| antibodies to a putative hepatitis c virus polyprotein in japanese patients with chronic liver disease. | to investigate the frequency of exposure to hepatitis c virus (hcv) in chronic liver disease, sera from japanese patients were tested with the original anti-hcv assay (ortho) and an anti-hcv assay based on synthetic peptides corresponding to a variety of regions in the hcv genome. thirty-one (67%) of 46 patients with chronic non-a,non-b hepatitis were anti-hcv-positive by the ortho elisa, 20 of whom were also positive by elisa based on synthetic hcv peptides. eight (53%) of the 15 patients negat ... | 1992 | 1334132 |
| [hepatitis c virus and serologic tests for detection of viral hepatitis c]. | | 1992 | 1334216 |
| improved detection of antibodies to hepatitis c virus in dialysis patients using a second-generation enzyme immunoassay. | we tested serum samples from 99 patients undergoing maintenance hemodialysis for hepatitis c virus (hcv) antibodies using a first-generation, licensed anti-hcv enzyme immunoassay (eia) and a second-generation anti-hcv eia that detect three gene products c100-3, ns3, and core. specimens that were repeatedly reactive by either or both screening assays were further evaluated by testing with supplemental eias and a dot blot immunoassay. there was 87.9% agreement between the licensed hcv eia and the ... | 1992 | 1334368 |
| [clinical diagnostic significance of hepatitis c]. | | 1992 | 1334498 |
| strategies for blood screening for the hepatitis c virus and for the human immunodeficiency virus in high risk groups. | for many infectious agents, seroprevalence rate is low but has serious consequences and must therefore be kept out of donated blood supplies. however, screening to ensure the safety of blood supplies has an associated very high cost. for example, in blood banks, detection of all the harmful items in a large number of samples is an expensive and tedious process. the laboratory and statistical approaches to obtain significant savings by the pooling method were discussed from 1943, recently, there ... | 1992 | 1334518 |
| second generation anti-hcv test: seroprevalence in hemodialysis patients and blood donors. | | 1992 | 1334595 |
| transmission of hepatitis c virus by kidney transplantation. | | 1992 | 1334596 |
| detection of replicative hepatitis c virus sequences in hepatocellular carcinoma. | several serologic studies suggest that infection by hepatitis c virus (hcv) may be associated with the development of hepatocellular carcinoma (hcc). therefore, we examined tumor tissue and/or the surrounding liver of 20 patients for viral sequences by the polymerase chain reaction (pcr). in 12 cases, liver and tumor tissues were separable for extraction. rna was extracted from frozen tissues and used as a template for reverse transcription followed by double pcr with nested primers for the 5'-u ... | 1992 | 1334635 |
| [essential mixed cryoglobulinemia and the hepatitis c virus]. | | 1992 | 1334715 |
| partitioning of hepatitis c virus during cohn-oncley fractionation of plasma. | because of concern about the safety of immune globulins with respect to transmission of hepatitis c, the partitioning of hepatitis c virus (hcv) during alcohol fractionation of a plasma pool prepared exclusively from anti-hcv-reactive donations was examined. quantitation of hcv rna was accomplished by nested polymerase chain reaction (pcr) at limiting dilutions. one pcr unit was arbitrarily defined as the minimum amount of hcv rna from which an amplified product could be detected. the starting p ... | 1992 | 1335184 |
| [the serodiagnosis of viral hepatitis c and b in different population groups]. | data on the frequency of detection of serological markers of hepatitis c and b viruses using enzyme immunoassay for examinations of blood donors in different regions of the country, of patients with hepatitis of obscure etiology and patients with hemophilia regularly receiving hemostatic therapy are presented. the rate of detection of antibody to hepatitis c (anti-hcv) in reserve donors varied from 2% (baltic states) to 9.2% (moldavia), that of the carrier state of hbsag from 0.3% to 10.3%, resp ... | 1992 | 1335191 |
| hepatitis c and non-a non-b hepatitis revisited: hepatitis e, f and g. | | 1992 | 1335466 |
| association of hepatitis c virus in human sera with beta-lipoprotein. | hepatitis c virus (hcv)-rna in sera of patients with viral hepatitis c is supposed to be included, at least partially, into hcv particles. we found that the density of hcv-rna-carrying material was variable, as determined by sucrose gradient density centrifugation (1.03-1.20 g/cm3). in some of the sera examined hcv-rna was restricted to low densities between 1.03 and 1.08 g/cm3. in other sera additional densities of hcv-rna were found distributed over the whole gradient with peaks at 1.12 and 1. ... | 1992 | 1335546 |
| full-length nucleotide sequence of a japanese hepatitis c virus isolate (hc-j1) with high homology to usa isolates. | | 1992 | 1335573 |
| hepatitis c antibodies in patients with alcoholic liver disease commonly have an identifiable risk factor. | hepatitis c virus (hcv) antibodies detected by radioimmunoassay (ria) and immunoblot assay were determined in 139 patients with alcoholic liver disease (ald). risk factors for exposure to hcv were recorded. anti-hcv was detected by ria in 38 (27%), but only 18 (13%) were confirmed with an immunoblot assay. one hundred seven (77%) had risk factors. of these 107, anti-hcv was detected in 36 by ria and in 17 by immunoblot assay. only 2 of 32 patients without risk factors had anti-hcv detected by ri ... | 1992 | 1336022 |
| recent advances in the study of hepatocellular carcinoma. | among the known causes for the occurrence of hepatocellular carcinoma (hcc), chemical carcinogens, chronic alcoholic intake and hepatitis b virus (hbv), especially in asia, has been emphasized. hbv has been industriously studied and many queries about the relationship between hbv infection and hepatocarcinogenesis have been clarified. recent discovery of hepatitis c virus (hcv) revealed that there may be the participation of this virus in hepatocarcinogenesis. however, a precise mechanism in suc ... | 1992 | 1336081 |
| [hepatitis c virus markers in the population quota assigned to a general medicine service]. | | 1992 | 1336087 |
| [hepatitis c; a new virus for an old disease]. | | 1992 | 1336130 |
| hepatitis c virus infection in kidney graft recipients: a study with second-generation assays. | | 1992 | 1336147 |
| proteins encoded in the 5' region of the pestivirus genome--considerations concerning taxonomy. | the first protein encoded within the pestivirus open reading frame is a nonstructural protein which removes itself from the polyprotein by autoproteolytic cleavage. the following nucleocapsid protein ends just before a putative signal sequence preceding three glycosylated proteins. all three glycoproteins are part of the viral envelope and exist in the form of disulfide-linked dimers. pestiviruses have recently been reclassified as members of the family flaviviridae which now comprises three gen ... | 1992 | 1336240 |
| virus inactivation of fresh frozen plasma by a solvent detergent procedure: biological results. | in order to increase the safety of blood products, we have developed a procedure for the virus inactivation of fresh frozen plasma. several batches have been prepared and with the first 10 batches, each of them composed of 60 litres of plasma, we have determined a set of biological parameters. virus inactivation was realised using tnbp (1%) and octoxynol 9 (1%). after their elimination with castor oil using chromatography on insolubilized c18 resin, glycine was added and the ph of the plasma was ... | 1992 | 1336258 |
| anti-hepatitis c virus immunoglobulin m antibody in patients with chronic hepatitis type c. | anti-hepatitis c virus immunoglobulin m antibody titers were measured by enzyme-linked immunosorbent assay in 28 patients with chronic hepatitis c. nine patients were treated with alpha-interferon at a dosage of 3 miu/day for 4 weeks. anti-hcv igm was positive in 12 of the 28 patients, and these 12 patients also had significantly higher anti-hcv antibody titers than those patients who were negative for anti-hcv igm. among the 9 patients treated with alpha-interferon, anti-hcv igm positivity was ... | 1992 | 1336473 |
| detection of hepatitis c virus rna by nested polymerase chain reaction in sera of patients with chronic non-a, non-b hepatitis treated with interferon. | to evaluate the effect of interferon-alpha treatment on the levels of serum aminotransferase (salt) and of hepatitis c virus (hcv)-rna, we studied 19 patients with chronic non-a, non-b (nanb) hepatitis. before therapy, 14 patients were positive by nested polymerase chain reaction (pcr) with primers deduced from the 5'-non-coding region of the hcv genome. serum hcv-rna had disappeared in 12 (85.7%) of them by the end of therapy, but then reappeared 6 months later in 4 of these 12 patients. a mark ... | 1992 | 1336504 |
| serum hepatitis c virus rna and hepatitis b virus dna in non-a, non-b post-transfusional and sporadic chronic hepatitis. | the sera of 36 french patients with post-transfusional and sporadic non-a, non-b chronic hepatitis were investigated, for hbv and hcv infections using a combination of serological and polymerase chain reaction (pcr) assays. anti-hcv was detected in 75% (27/36) of the patients by elisa1 and/or riba2 tests. hcv-rna sequences were found in 75% (27/36) of the sera by a single step pcr, using a set of primers located in the 5' non-coding region. altogether, 89% (32/36) of the patients were found posi ... | 1992 | 1336507 |
| detection of hepatitis c virus (hcv) rna sequences in liver tissue by in situ hybridization. | in situ hybridization was used to identify the cell types infected by hepatitis c virus (hcv) in the liver. using an antisense hcv-rna probe from the 5' non-coding region, hcv-rnas molecules were detected in liver sections of 4/11 patients with chronic hepatitis c. these 4 positive subjects were also infected by hiv. hcv-rna-positive strands were detected in scattered hepatocytes as well as in cells identified as mononuclear cells within the inflammatory infiltrates. hcv-rna negative strands, li ... | 1992 | 1336509 |
| hepatitis c virus, autoimmune liver disease and cryoglobulinaemic hepatitis. | | 1992 | 1336510 |
| [survey of the antibody to hcv in national leprosarium suruga]. | blood specimens from 210 leprosy patients (average age 67.4 years old) and 84 staff members (average age 43.5 years old) in national leprosarium suruga were tested for anti-hcv antibody using ortho's ab elisa system. among the patients, 17 patients had chronic hepatic dysfunction as well as leprosy. twenty of the 210 patients (9.5%) had anti-hcv antibody in their blood. eleven of the 17 patients (65%) with chronic hepatic dysfunction were positive for anti-hcv antibody. only one of the staff mem ... | 1992 | 1336773 |
| replication of hepatitis c virus in peripheral blood mononuclear cells. effect of alpha-interferon therapy. | hepatitis c virus (hcv) is a positive-stranded rna virus which replicates through a negative-stranded rna intermediate. using a pcr procedure to detect positive and negative strands, we investigated the existence of hcv replication in lymphoid cells. both positive and negative strands were found in the peripheral blood mononuclear cells (pbmc) of all patients (n = 10) with untreated chronic hepatitis c. no hcv sequences were detected in pbmc in any of the 8 healthy controls. fifteen patients wit ... | 1992 | 1336787 |
| anti gor and hepatitis c virus in autoimmune liver disease. | | 1992 | 1336907 |
| hepatitis c in chronic renal failure patients. | the occurrence of hepatitis c virus (hcv) infection amongst chronic renal failure (crf) patients in our nephrology unit was investigated over a period of 1 year. a total of 71 patients was studied comprising 26 chronic haemodialysis (chd) patients, 6 acute haemodialysis patients, 4 peritoneal dialysis patients and 35 crf patients not on dialysis. patients were screened before and after haemodialysis, and their baseline and postdialysis values of liver enzymes were determined. eleven (15.5%) of t ... | 1992 | 1336933 |
| [the role of hbv and hcv viruses in the pathogenesis of hepatic lesions in alcoholics]. | due to the high prevalence in the population of both viral hepatitis and alcoholism it is not uncommon to find patients with chronic hepatitis in whom both these stigmata are simultaneously present. in such cases, the risk of evolution to cirrhosis is thought to be greater. in this study was evaluated 104 alcoholic patients (50 of whom had hepatic biopsy), with or without antibodies (ab) to hepatitis c virus (hcv). in addition, we retrospectively examined 183 hepatic biopsies of alcoholic patien ... | 1992 | 1337153 |
| [drug addiction and hepatitis c: does a correlation exist between the laboratory and clinical pictures?]. | the prevalence of anti-hcv antibodies reaches 90% positive among intravenous drug addicts. the authors evaluate the possibility of relating laboratory findings to clinical evolution of hepatitis c. besides, a comparison between first generation anti-hcv system elisa and the newer riba detection system showed no significant difference. | 1992 | 1337387 |
| polymerase chain reaction assay for hepatitis c virus rna using a single tube for reverse transcription and serial rounds of amplification with nested primer pairs. | a procedure is described for the detection of hepatitis c virus (hcv) rna in blood by means of the polymerase chain reaction (pcr) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. this results in fewer manipulations, reduced risk of contamination, and economy of time. the procedures are generally applicable to other assays based on the pcr. we describe the preparation (from 100 microl serum) of test samples that remain stable for at least ... | 1992 | 1337550 |
| hepatitis c virus testing of sera in africa. | | 1992 | 1337635 |
| viral infections in a sample of intravenous drug abuser prisoners. | the authors point out the high frequency of viral infections in a sample of ivdas. the most significant infections that emerged are cmv and hcv diseases, in contrast with other epidemiological studies. | 1992 | 1338122 |
| [detection of hcv-rna copies in sera in patients with chronic hepatitis c]. | | 1992 | 1338209 |
| hepatitis c: molecular biology, pathogenesis, epidemiology, clinical features, and prevention. | | 1992 | 1338348 |
| anti-hcv antibodies and hepatocellular carcinoma. relationship in a medium-risk population. | by means of an accurate immunoenzymatic assay, the prevalence was studied of antibodies to hepatitis c virus (hcv) in three different populations: 74 patients affected with hepatocellular carcinoma (hcc) on preexisting cirrhosis, 82 patients with liver cirrhosis but with no apparent neoplasm, and 70 control subjects, hospitalized for various conditions, of internal medicine or geriatric interest. 70.2% of hcc patients exhibited anti-hbc antibodies, versus 47.5% of cirrhotic subjects with no tumo ... | 1992 | 1338660 |
| [hepatitis c]. | | 1992 | 1338677 |
| [the causative agents of hepatitis non-a, non-b]. | | 1992 | 1338779 |
| [antibodies against hepatitis c virus. study in voluntary blood donors. cuba 1991]. | four hundred and sixty one samples randomly selected among blood donors from three blood banks in havana city were investigated for hcv antibodies. samples repeatedly reactive to organcon tecknica immunoenzymatic assay were considered positives and were further confirmed by an ortho test. an epidemiological survey was conducted among all donors included in the sample to assess infection risk factors. seven subjects (1.5%) resulted repeatedly reactive to the test, slightly higher than in develope ... | 1992 | 1339033 |
| [current status in hepatitis virus research]. | seroconversion from eag to anti-e is frequently seen in the course of chronic hepatitis b infection. this phenomenon is closely related to mutation of the precore region; a g-to-a substitution of nucleotide position 1986 replaces tryptophan to translational stop codon. this mutant is responsible for the fulminant hepatitis or acute exacerbation of chronic hepatitis b. the hepatitis c virus shows a high rate of genome variations, especially at the envelope (e and ns1) region, where a neutralizing ... | 1992 | 1339164 |
| [incidence of hepatitis c virus in kidney transplantation recipients]. | the investigation was carried on 122 waiting renal transplantation hospitalized patients. detection of hcv antibodies was done before transplantation and after renal transplantation. hcv antibodies were detected by immunosorbent assay (elisa) for c 100-3 protein of hc virus (lab. ortho). positive results were checked by a second test (immunoblot riba ii) to detect antibodies against c100-3, 5-1-1, c33, c22 proteins of hcv genome. before transplantation, 112 patients were negative and 10 positive ... | 1992 | 1340101 |
| [the prevalence of anti-hepatitis virus c antibodies in chronic hemodialysis patients]. | liver involvement with a variety of viral diseases is a frequent finding in chronic renal failure patients on regular hemodialysis treatment. we evaluated the prevalence of igg anti-hepatitis c virus antibodies (hvc) in our dialysis unit, looking for risk factors associated with seropositivity and we assessed the type and degree of liver involvement by means of a liver biopsy in those patients with biochemical abnormalities of liver function test. we studied 50 patients aged 13 to 77 years, and ... | 1992 | 1340899 |
| detection of hepatitis c virus rna sequences by polymerase chain reaction in fixed liver tissue. | currently, the most reliable method for the diagnosis of hepatitis c virus (hcv) infection is the detection of viral sequences by the reverse transcription double polymerase chain reaction (rt/pcr) in serum or liver samples. we demonstrate here that noncoding region sequences (nt) of hcv were amplifiable by rt/pcr in guanidinium extracts of formalin-fixed (for 6 to 48 h), paraffin-embedded liver sections of patients with chronic hepatitis c. in contrast, core and nonstructural region sequences o ... | 1992 | 1344813 |
| anti-gor and hepatitis c virus in autoimmune liver diseases. | anti-gor is an autoantibody found in hepatitis c virus (hcv) infection. we have studied the specificity of this antibody for hcv infection in various groups of autoimmune liver diseases. anti-hcv was detected by a second generation hcv enzyme-linked immunosorbent assay in 14 of 29 patients with liver-kidney-microsomal (lkm-1) -antibody-positive autoimmune hepatitis type 2 and in all 6 control patients with hcv-rna-positive chronic hepatitis c. anti-hcv was not found in those with antinuclear-ant ... | 1992 | 1346282 |
| lymphocytic sialadenitis of sjögren's syndrome associated with chronic hepatitis c virus liver disease. | viral infection has often been suggested as a possible cause of sjögren's syndrome or chronic lymphocytic sialadenitis, and epstein-barr virus has been found in the salivary glands of patients with this condition. after we had noted sjögren's syndrome in several patients infected with hepatitis c virus (hcv), a virus also excreted in saliva, we set up a prospective study to investigate the association of chronic lymphocytic sialadenitis, with or without symptoms, to chronic hcv liver disease. th ... | 1992 | 1346409 |
| hcv confirmatory testing of blood donors. | | 1992 | 1346452 |
| hepatitis c virus, a causative infectious agent of non-a, non-b hepatitis: prevalence and structure--summary of a conference on hepatitis c virus as a cause of hepatocellular carcinoma. | | 1992 | 1346540 |
| need for second-generation anti-hcv testing in haemophilia. | | 1992 | 1346863 |
| history of previous drug misuse in hcv-positive blood donors. | | 1992 | 1346864 |
| hcv genotypes in different countries. | | 1992 | 1347826 |
| hcv confirmatory testing of blood donors. | | 1992 | 1348314 |
| hcv confirmatory testing of blood donors. | | 1992 | 1348316 |
| hepatitis b virus and apparent fulminant non-a, non-b hepatitis. | while there is evidence that hepatitis c virus (hcv) does not cause fulminant non-a, non-b hepatitis, the causal agent remains unknown. to evaluate the role of hepatitis b virus (hbv) in this disease, we used a two-step polymerase chain reaction (pcr) to amplify the surface and core regions of hbv dna in serum and liver samples taken prospectively from twenty-six patients (mean age 36 years, range 1 to 64) with acute hepatic failure undergoing liver transplantation. hbv dna was absent from the s ... | 1992 | 1348798 |
| hepatitis c virus and mixed cryoglobulinaemias. | | 1992 | 1348816 |
| sensitivity of serological assays to identify blood donors with hepatitis c viraemia. | blood donors at high risk of hepatitis c virus (hcv) infection were tested for viraemia by the polymerase chain reaction (pcr). pcr results were accepted as positive only if reactive in 3 of 4 tests and if confirmed in an independent laboratory. the sera were also tested by 6 different assays to determine the ability of current serological assays to detect viraemic blood donors. of 19 pcr-positive sera, only 13 (68%) were detected by the most sensitive of the serological assays. if these results ... | 1992 | 1349053 |
| hcv and sjögren's syndrome. | | 1992 | 1350842 |
| contamination of immunoassay controls with hepatitis c virus. | | 1992 | 1351575 |
| detection of antibodies to hepatitis c virus in urine. | | 1992 | 1351576 |
| evaluation of anti-hcv capsid indeterminate serum samples. | | 1992 | 1353178 |
| detection of hcv in seronegative donors with raised alt. | | 1992 | 1353179 |
| transmission of hepatitis c with pasteurised factor viii. | | 1992 | 1353214 |
| hepatitis c viraemia and liver disease in symptom-free individuals with anti-hcv. | there is controversy about clinical management of patients who persistently have antibodies to hepatitis c virus (anti-hcv) but who have no symptoms and signs of liver disease. we have taken liver biopsy samples from 23 such patients (16 of whom had normal alanine aminotransferase [alt] values) to assess prevalence of liver disease and to see whether anti-hcv and hcv-rna correlated with histological findings. 16 patients had histological evidence of chronic hepatitis, which was not predicted by ... | 1992 | 1355801 |
| blood donation. | | 1992 | 1357379 |
| low cost anti-hcv screening of blood donors. | | 1992 | 1357481 |
| passively transfused hepatitis c antibody. | | 1992 | 1359346 |
| hepatitis c virus and porphyria cutanea tarda: evidence of a strong association. | porphyria cutanea tarda in human beings is believed to be due to reduced hepatic uroporphyrinogen decarboxylase activity. however, extrinsic factors such as alcohol abuse and drug intake are required for clinical manifestation of the disease. in addition to typical cutaneous lesions, patients with porphyria cutanea tarda usually have chronic liver disease and moderate iron overload. of 74 italian patients with porphyria cutanea tarda, hepatitis c virus antibodies were detected in 76% by enzyme-l ... | 1992 | 1359994 |
| correlation between elisa and recombinant immunoblot assay in serum samples positive for anti-hcv. | to determine the false-positivity of antibody to hepatitis c virus (hcv) (anti-c100-3), the correlation between elisa optical density (od), anti-hcv titer and recombinant immunoblot assay (riba) in 87 anti-hcv-positive sera was analyzed. the results showed that > 90% of the serum samples with an anti-hcv od > 2.0 or with titers > 10 x were riba reactive. these findings are applicable in daily clinical practice. | 1992 | 1360305 |
| vertical transmission of hepatitis c virus infection. | | 1992 | 1360919 |
| molecular cloning and sequence analysis of an isolate of hepatitis c virus from southern california, u.s.a. | we obtained a cdna clone representing a partial rna sequence of a human hepatitis c virus (hcv) isolate from southern california. rna was extracted from the liver tissue of a patient with post-transfusion hepatitis, and used for cdna synthesis and polymerase chain reaction (pcr) amplification using oligonucleotide primers specific for the prototype hcv rna. the cdna clone obtained contains 585 base pairs of hcv sequences and represents the region encoding the putative nonstructural protein ns3 o ... | 1992 | 1364214 |
| [hepatitis c viral infection and the consumption of intravenous drugs]. | to know the antibodies seroprevalence against hepatitis c virus (hcv), as well as other factors associated to these antibodies presence in a group of intravenous drug users (ivdu) in baleares. to know what screening test would be the most adequate to detect antibodies against hcv in this group. | 1992 | 1366219 |
| effects of oxygen on recombinant protein production by suspension cultures of spodoptera frugiperda (sf-9) insect cells. | spodoptera frugiperda (sf-9) insect cells have been grown in serum-free medium in 250-ml spinner flasks. the maximum cell density obtained in these cultures was dependent on the aeration rate of the culture. similar yields of uninfected cells were obtained when cultures were stirred in spinner flasks at 80 rev min-1 and in a 4-1 stirred-tank bioreactor and the dissolved oxygen in the bioreactor was controlled at 20% of air saturation. cells were infected with a recombinant baculovirus at differe ... | 1992 | 1369406 |
| detection of hepatitis c virus rna in serum of patients with chronic hepatitis c treated with interferon-alpha. | we tested serial serum samples for hepatitis c virus rna from patients undergoing treatment for chronic hepatitis c with interferon-alpha using an assay that combined reverse transcription and polymerase chain reaction. the subjects studied were 20 patients with chronic hepatitis who had serum antibody to hepatitis c virus (anti-c100-3). before therapy, hepatitis c virus rna was detected in 18 (90%) and 20 (100%) patients using primer sets derived from the ns3 region or the 5'-noncoding region o ... | 1992 | 1370161 |
| seroprevalence of hepatitis c virus nucleocapsid antibodies in patients with cryptogenic chronic liver disease. | the serological responses to two different hepatitis c virus antigens were studied by enzyme-linked immunosorbent assay in a variety of chronic liver diseases and in healthy blood donors. the study population comprised 97 cases of cryptogenic chronic liver disease (40% with a history suggestive of parenterally transmitted non-a, non-b hepatitis and 60% without such a history), 87 cases of other well-characterized chronic liver diseases and 96 voluntary blood donors. the commercially available c1 ... | 1992 | 1370945 |
| nucleotide sequences of 5-1-1 of hepatitis c virus in patients with chronic liver disease. | the discovery of hepatitis c virus (hcv) has led to the development of an assay against a viral peptide (c100-3), which is now used worldwide. it has been shown that the majority of hcv-infected individuals are positive for the antibody. however, there are patients who are repeatedly seronegative for the antibody despite the presence of hcv rna in the serum. the nucleotide sequences of 5-1-1 (a major epitope of c100-3) obtained from five antibody-positive patients and five negative patients with ... | 1992 | 1371485 |
| reactivity to c33c antigen as a marker of hepatitis c virus multiplication. | | 1992 | 1371538 |
| hepatitis c antibody in renal transplant patients. | | 1992 | 1371619 |
| clinical implications of the presence of antibodies to hepatitis c after renal transplantation. | | 1992 | 1371627 |
| improved serodiagnosis of non-a, non-b hepatitis by an assay detecting antibody to hepatitis c virus core antigen. | we examined sequential serum samples from 12 patients with well-characterized posttransfusion non-a, non-b hepatitis who had an acute, resolving self-limited type of clinical course for the presence of antibody to the hepatitis c virus nucleocapsid (core) protein (p22) expressed by a recombinant baculovirus. these sera were simultaneously examined for antibody to the hepatitis c virus nonstructural protein (c100-3) that is presently used for blood screening worldwide. in three patients, both ant ... | 1992 | 1371981 |
| the development of hepatitis c antibody shortly after acute icteric non-a non-b hepatitis. | to determine the relationship between the development of hepatitis c antibody (anti-hcv) and the clinical symptoms in acute hepatitis c. | 1992 | 1372078 |
| hepatitis c virus and organ donor cards. | | 1992 | 1372193 |
| antibodies to hepatitis c virus in blood donors. | | 1992 | 1372593 |
| serological markers of posttransfusion hepatitis c viral infection. | serological markers for hepatitis c virus (hcv) infection were measured in serial samples from 14 posttransfusion chronic non-a, non-b hepatitis patients by a semiquantitative dot blot immunoassay. the assay detected antibodies to hcv by use of recombinant proteins that represent putative hcv capsid (core), nonstructural protein 3 (ns3) (33c), and ns4 (c100) epitopes. seroconversion to anti-hcv antibodies (anti-hcv) was detected in all patients. the average time to active antibody production det ... | 1992 | 1372618 |
| the prevalence of antibody to hepatitis c virus in a dialysis population. | | 1992 | 1373230 |
| prevalence of anti-hepatitis c virus antibodies in positive fta-abs non-drug abusing female prostitutes in spain. | the prevalence of antibodies against hepatitis c virus (anti-hcv antibodies) and its association with the presence of treponemal antibodies (fta-abs) was studied in 227 non-drug abusing female prostitutes. the overall anti-hcv antibody prevalence was 8.8%, and a significantly higher prevalence of anti-hcv antibodies was found in prostitutes testing positive for fta-abs (19.4%) than in prostitutes testing negative for fta-abs (3.9%) (pr = 5.023, p less than 0.001). no statistical association was ... | 1992 | 1373253 |
| detection of hepatitis c virus-specific antigens in semen from non-a, non-b hepatitis patients. | semen samples from nine patients clinically diagnosed as having non-a, non-b hepatitis (nanbh) were tested by an elisa using antibodies raised in rabbits against hcv-specific antigens. the semen from all nine patients had elevated levels of hcv-specific antigen in comparison to semen from five healthy donors. semen from five of the nine patients had significant levels of the hcv-specific antigen. seven of the eight serum samples from these patients were reactive with the standard c-100 hcv elisa ... | 1992 | 1373358 |