Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| transfusion-associated non-a, non-b hepatitis: an assessment of the causative agent and its clinical impact. | 1988 | 2856531 | |
| discovery of the non-a, non-b hepatitis virus: the end of the beginning or the beginning of the end. | 1989 | 2562477 | |
| localization of hepatitis c virus (hcv) antigen by immunohistochemistry on fixed-embedded liver tissue. | an immunohistochemical method of staining hcv-related antigens in fixed-embedded liver biopsies is described. two primary antisera were used: 1) a high titre anti-hcv human igg separated from an anti-hcv positive serum; and 2) rabbit anti-hcv antibodies. in our experience this method proves to be reproducible and shows a good correlation with serologic results. | 1990 | 1720059 |
| [detection of non-a, non-b hepatitis viral gene by polymerase chain reaction]. | 1990 | 1962866 | |
| [treatment of cryptogenic chronic liver diseases with recombinant alpha-2a interferon. preliminary results of a randomized controlled clinical trial]. | fifty-six consecutive patients with cryptogenic chronic active liver disease were enrolled in a randomized controlled clinical trial of alpha-2a interferon and randomly assigned to a control group (28 patients) and to a treated one (28 patients). all had a histologic diagnosis of chronic active hepatitis (with superimposed cirrhosis in 50% of them) and presented a persistent elevation in aminotransferases, during the last year. ad-interim analysis shows that 19 out of 28 treated patient (68%) ha ... | 1990 | 1963071 |
| phenprocoumon-induced hepatitis mimicking non-a, non-b hepatitis. | a 58-year-old man presented with jaundice 6 months after aortic valve replacement. although non-a, non-b hepatitis was initially suspected, the final diagnosis of phenprocoumon (marcoumar)-induced hepatitis progressing to cirrhosis was based on recurrence of jaundice after re-exposure to the drug, improvement after withdrawal and centrilobular necrosis with eosinophilic infiltration in the liver biopsy. antibodies to hepatitis c virus were absent. the aortic valve was replaced by a bioprosthesis ... | 1990 | 1963177 |
| prevalence of antibodies to hepatitis c in different etiologies of cirrhosis. | 1990 | 1963179 | |
| detection of hepatitis c virus rna by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5'-noncoding region. | the 5'-noncoding region of hepatitis c virus (hcv) genomes is highly conserved. a two-stage polymerase chain reaction (pcr), involving two pairs of primers deduced from the 5'-noncoding region of the hcv genome, was developed for a sensitive and specific detection of hcv rna. the first stage of pcr was performed for 35 cycles with primers capable of multiplying fragments of 221 base pairs. pcr products in samples negative for hcv rna were subjected to the second stage of pcr for 30 cycles with p ... | 1990 | 1963453 |
| enzyme-linked immunosorbent assay for antibodies against the capsid protein of hepatitis c virus with a synthetic oligopeptide. | an enzyme-linked immunosorbent assay was developed for the determination of antibodies against the putative capsid protein of hepatitis c virus (hcv). a 36-mer oligopeptide with a sequence of rrgprlgvratrktsersqprgrrqpipkvrrpegr (cp9) was synthesized; it was selected on the translation product of the presumptive hcv core gene, because of a high local hydrophilicity and excellent conservation by different hcv strains. the synthetic peptide was immobilized on a solid-support to capture antibodies ... | 1990 | 1963454 |
| blood protein derivative viral safety: observations and analysis. | the well-documented viral safety of albumin arises from several factors operating in concert, including virus removal during preparation, immune neutralization, serendipitous inactivation, virus sterilization through pasteurization. safety with respect to hbv transmission was achieved even prior to the development of sensitive screening tests for hbsag, as can be predicted given the initial virus load and the influence of factors affecting removal and inactivation. coagulation factor concentrate ... | 1990 | 1963504 |
| seroepidemiologic study of hepatitis b and c viruses in federal correctional institutions in british columbia. | 1990 | 1963577 | |
| sporadic acute hepatitis in hospital employees: mainly non-a, non-b type. | eighty-two patients with sporadic acute viral hepatitis were detected at okayama university hospital between january 1982 and march 1989. thirty of them had non-a, non-b hepatitis (nanbh); 20 had hepatitis a (ha); and 32 had hepatitis b (hb). ten (33%) of nanbh, five (25%) of ha, and nine (28%) cases of hb were hospital employees. two (20%) of 10 hospital employees with nanbh developed chronic hepatitis. antibody to hepatitis c virus (anti-hcv, elisa) was tested in 10 patients with nanbh (five h ... | 1990 | 1963730 |
| antibodies to hepatitic c virus and chronic liver disease among finnish patients with haemophilia. | antibodies to hepatitis c virus, hepatitis b serology and liver enzymes were examined in 137 finnish haemophiliac patients to detect signs of chronic viral hepatitis and its possible aetiological associations. the prevalence of raised alanine aminotransferase values was 37%. these were significantly associated with hepatitis c seropositivity but not with hepatitis b antibodies, severity of haemophilia or the type of clotting factor used in replacement therapy. the prevalence of hepatitis c serop ... | 1990 | 1963789 |
| virology without virus? | 1990 | 1963882 | |
| sequence diversity of hepatitis c viral genomes. | the nucleotide sequences of cdnas (275 base-pairs) in the non-structural protein 5 regions of japanese isolates of hepatitis c virus (hcv-j) from the plasma of 11 patients with non-a, non-b hepatitis and the livers of five patients with hepatocellular carcinoma were analyzed. approximately 14 to 17% of nucleotide sequences of the hcv-js examined differed from that of the original isolate in the united states (hcv-us). furthermore, 2.5 to 11% sequence diversity was found among the hcv-js. the nuc ... | 1990 | 1963917 |
| clinical, virological and epidemiological basis for the treatment of chronic non-a, non-b hepatitis. | non-a, non-b hepatitis is the most common serious complication of blood transfusion and also occurs in a sporadic form whose routes of transmission are currently unknown. while generally mild in its acute presentation, non-a, non-b hepatitis frequently progresses to chronic hepatitis which may eventuate in cirrhosis and hepatocellular carcinoma. the disease is caused by a small, enveloped rna virus now designated hepatitis c virus, which has similarities to the flaviviruses. studies using the re ... | 1990 | 1964164 |
| prevalence of hepatitis c virus infection in africa: anti-hcv antibodies in the general population and in patients suffering from cirrhosis or primary liver cancer. | anti-hepatitis-c-virus (anti-hcv) antibody was tested for in sera from 410 adults living in tunisia, senegal, burundi and madagascar, and in 209 tunisian and senegalese patients suffering from liver diseases. anti-hcv antibodies were detected in 4.2% of the adult population from africa, in 51% of patients suffering from liver cirrhosis and in 37% of patients suffering from primary liver cancer. however, higher proportions of anti-hcv antibodies were detected in hbsag+ patients than in hbsag- pat ... | 1990 | 1964239 |
| low prevalence of antibody to hepatitis c virus in north east england. | the prevalence of antibodies to hepatitis c virus (anti-hcv) was studied in north east england in blood donors, local multiply transfused patients, local high risk individuals, and chronic liver disease patients. anti-hcv was detected by enzyme-linked immunosorbent assay (elisa) in 2/1120 (0.18%) blood donors; 1/84 chronic renal failure patients on haemodialysis who had received 1,992 units of blood (seroconversion rate of 0.05% per unit transfused), 1/207 cardiac patients 6 months post cardiac ... | 1990 | 1964476 |
| effectiveness of a preventive programme for non-a, non-b hepatitis in a large dialysis unit. | 1990 | 1965018 | |
| loss of antibodies against hepatitis c virus in hiv-seropositive intravenous drug users. | a time-point survey of 262 ex-intravenous drug users (ivdus) on methadone treatment showed serological evidence of hepatitis c virus (hcv) in 64%, hepatitis b virus in 74% and hiv in 36%. analysis of previously collected frozen stored sera of the same patients showed that 31 further ivdus had had anti-hcv antibodies in the past and had lost them in a time-dependent fashion. most hcv seroreversion was detected in hiv-positive people: 20 out of 85 in the hiv-positive group versus 11 out of 18 in t ... | 1990 | 1965126 |
| hepatitis c and e. | 1990 | 1965321 | |
| [diagnosis and epidemiology of hepatitis c]. | after years of unsuccessful attempts to isolate the causative agent of hepatitis non-a-non-b this has recently been accomplished. the nucleic acid of a virus, now called hepatitis c, was cloned which formed the basis of a diagnostic elisa. in this test antibodies against a non-structural protein of the virus can be detected. these antibodies are not actually protective but rather indicate virus activity. the first generation test is hampered by some unspecificity but mostly by the fact that sero ... | 1990 | 1965468 |
| viral safety and inhibitor development associated with factor viiic ultra-purified from plasma in hemophiliacs previously unexposed to factor viiic concentrates. the monoclate study group. | a multicenter study evaluated the potential for transmitting non-a/non-b hepatitis, as well as other viruses, with the use of the factor viiic product monoclate. this product is purified from plasma via the monoclonal process that includes heat treatment for ultra-purification as a final step. twenty different lots of monoclate were used, and each patient received the assigned lot for the first 6 months of the trial. nineteen of 38 patients adhered strictly to international committee on thrombos ... | 1990 | 1965615 |
| prevalence and significance of anti-hcv antibodies in kidney graft recipients. | 1990 | 1965737 | |
| hepatitis c infection in hemodialysis units. | we performed an epidemiological study of the hepatitis c infection on 112 patients of 3 urban hemodialysis units using a recently developed anti-hcv recombinant based assay. eleven patients (9.8%) were positive for anti-hcv. among them, 8 (72.7%) were positive for anti-hbc, one of whom was hbsag positive and 6 of whom were also anti-hbs positive. surprisingly, all of the anti-hcv (+) patients were normal alanine aminotransferase. the mean age of the anti-hcv (+) patients was 50.7 +/- 3.3 (mean + ... | 1990 | 1965882 |
| aetiological association of virus like particles with hepatitis c. | isolation of virus-like particles from sera of anti-hcv positive patients and their ultrastructural characterization are reported. particles were identified in sera of 5 out of 6 patients tested. immunoelectron microscopy assay revealed small aggregates of viral particles. size and morphological criteria suggest that these particles can be classified as togaviridae. | 1990 | 1966067 |
| the hepatitis c virus and its relationship to the clinical spectrum of nanb hepatitis. | 1990 | 1966485 | |
| prevalence of antibody to hepatitis c virus (anti-hcv) in chronic liver diseases (cld) in southern italy. | two hundred and sixty-three patients consecutively admitted to our unit for cld were investigated for the antibody to hepatitis c virus (anti-hcv) in the serum using the recently developed enzyme immunoassay. the overall prevalence of anti-hcv was 45%; in patients with cryptogenic cld it was significantly higher (69%) than in patients with markers of viral hepatitis (15%). anti-hcv was found in 62% of the patients with hepatocellular carcinoma; this finding favours a potential role of hcv in det ... | 1990 | 1966702 |
| high prevalence of co-occurrence of anti-hcv and anti-hbc antibodies in chronic hepatitis patients from southern italy. | the prevalence of anti-hcv and anti-hbv antibodies was investigated in a series of 77 adult patients with hbsag negative, non autoimmune chronic liver disease. anti-hcv were found in 68% and anti-hbc in 71% of the cases; 68% of the patients carried both antibodies. there was no difference in the proportion of anti-hbc positive cases between the anti-hcv positive or negative patients. the intense circulation of hbv infection in southern italy during the past decades might explain the high percent ... | 1990 | 1966708 |
| familial clustering of hepatitis c virus infection and chronic liver disease. | the present study was undertaken to assess the prevalence of hcv antibodies (anti-hcv) and of chronic liver disease in relatives of anti-hcv positive subjects suffering from chronic active liver disease. we studied 122 subjects from 24 families. each family had at least one positive anti-hcv component with histologically proven chronic liver disease. anti-hcv was found in 32% of subjects; 82% of these were suffering from chronic liver disease diagnosed on the basis of physical examination and bi ... | 1990 | 1966878 |
| [anti-hepatitis c viral antibodies in different pathological entities in chile]. | in order to determine the prevalence of anti-hepatitis c viral antibodies in chile, we determined antibody titers in preserved serum from 91 subjects using an elisa method. the number and percentage of patients with positive titers was as follows: hemophiliacs without evidence of liver disease 88% (n = 25); prostitutes 0% (n = 25); sporadic non a non b hepatitis 0% (n = 12); chronic non alcoholic hepatitis with negative anti-hbsag and anti hbc 63% (n = 10); hepatomas with negative titers as indi ... | 1990 | 1967082 |
| [hepatitis c virus]. | 1990 | 1967084 | |
| [hepatitis c in venezuela. preliminary report]. | five hundred serum samples from volunteers blood donors were investigated in order to determine the presence of anti-hcv antibodies by an enzyme-linked immunoassay recently developed worldwide. prevalence of true-positive samples was 1.2% (6/500), 2 fold higher than the reported prevalence in most of the industrialized countries. from these 6 sera, one (16.6%) showed simultaneous reactivity for hbv anticore antibody. three sera (25%) from 12 patients with diagnosis of non-a non-b hepatitis were ... | 1990 | 1967086 |
| [antibodies against hepatitis c virus in patients with liver diseases and in risk subjects. preliminary report]. | the recent discovery of an antigenic component of the causative agent of non-a, non-b hepatitis, has led to the characterization of this virus--hepatitis c virus (hcv)--and to the identification of an antibody present in infected subjects (anti-hcv) detected by means of the c-100 antigen derived from a nonstructural region of the viral genome. using a commercial kit (ortho diagnostic inc.), the incidence of anti-hcv antibody was studied in the military hospital "dr. carlos arvelo" of caracas, ve ... | 1990 | 1967087 |
| [prevalence of the antibody of hepatitis c virus in hbsag negative patients with chronic liver diseases: children, prostitutes, and homosexuals]. | we present the results of the determination of hepatitis c virus antibody, recently discovered on a group of 34 patients with chronic liver disease, divided in: a) 23 patients with cirrhosis. b) 8 with protracted hepatitis. c) 2 with steatosis of unknown origin++. d) one patients with hepatocellular carcinoma. another group examined was integrated by persons of promiscuous sexual activity (15 prostitutes and 14 homosexuals). finally we included 20 children chronically infected with vb. in the 23 ... | 1990 | 1967088 |
| washing with 8 mol/l urea to correct false-positive anti-hcv results. | 1990 | 1976899 | |
| enhanced detection by pcr of hepatitis c virus rna. | 1990 | 1976900 | |
| demonstration of viraemia patterns in haemophiliacs treated with hepatitis-c-virus-contaminated factor viii concentrates. | sequential serum samples from previously untreated haemophiliacs in whom non-a, non-b hepatitis (nanbh) developed after they received factor viii concentrate contaminated with hepatitis c virus (hcv) were tested by a commercial assay for the presence of antibodies to the c100 protein of hcv (anti-c100) and by "nested" pcr for the presence of hcv rna sequences. three temporal patterns of viraemia were observed: transient viraemia in acute resolving nanbh; viraemia lasting for several years in chr ... | 1990 | 1977017 |
| proceedings of the international symposium on molecular biology of hepatitis b virus and non-a non-b hepatitis virus. june 1, 1989, kanazawa, japan. | 1990 | 1977652 | |
| the a to f of viral hepatitis. | 1990 | 1978030 | |
| is anti-hcv blood donor screening useful? | 1990 | 1978051 | |
| hepatitis c quantification and sequencing in blood products, haemophiliacs, and drug users. | the polymerase chain reaction (pcr) detected specific hepatitis c viral (hcv) rna sequences in plasma from 15 of 21 haemophiliacs (12 hcv-antibody positive) and 7 of 27 intravenous drug users (13 hcv-antibody positive). quantification of rna-positive samples showed high levels of hcv (10(5) to 10(6) copies of rna/ml) in infected patients. hcv was more frequently found in haemophiliacs infected with human immunodeficiency virus (11/11 hiv-positive and 4/10 hiv-negative patients). hcv-rna was dete ... | 1990 | 1979093 |
| hepatitis c virus rna in saliva. | 1990 | 1979130 | |
| specificity of anti-hcv elisa assessed by reactivity to three immunodominant hcv regions. | 1990 | 1979411 | |
| [markers of hepatitis b and hepatitis c viruses in blood donors with positive or indeterminate immunoblot tests for human retroviruses]. | 1990 | 2148389 | |
| hepatitis c virus infection in sexually promiscuous groups. | as there is insufficient data available on the spread of hepatitis c virus (hcv) infection by sexual transmission, the prevalence of anti-hcv was determined in several sexually promiscuous groups (78 female prostitutes, 55 clients of prostitutes and 146 homosexual men) who denied intravenous drug abuse. the overall hcv infection rate was much higher in the sexually promiscuous groups (8.97%, 16.36% and 5.48% respectively) than in voluntary blood donors (0.48%), suggesting that hcv infection can ... | 1990 | 2170134 |
| prevalence of antibodies against hepatitis c virus in norwegians with congenital coagulation factor defects treated with plasma products from small pools. | the prevalence of antibodies against hepatitis c virus (hcv) in sera from 266 norwegians with coagulation factor defects of different types and degrees of severity was assessed by an enzyme immunoassay. the overall prevalence was 41%, the highest rates being found in persons with severe hemophilia a (64%) or b (67%). these prevalence rates are below those found in hemophiliacs in most other countries in the western hemisphere. this may be due to the strategy for coagulation factor substitution u ... | 1990 | 2170135 |
| implications of anti-hepatitis c reactivity in 'autoimmune' chronic active hepatitis. | 1990 | 2170224 | |
| hepatitis c viral cdna clones isolated from a healthy carrier donor implicated in post-transfusion non-a, non-b hepatitis. | using a specific hepatitis c virus (hcv) antibody assay, positive blood donors responsible for the transmission of post-transfusion non-a, non-b hepatitis (pt-nanbh) were identified. cdna fragments were isolated from one of the plasma samples of such healthy hcv carriers by using polymerase chain reactions. nucleotide (nt) sequence analyses of the cdna from three different regions of the viral genome revealed that they were derived from a japanese hcv isolate that was similar but not identical t ... | 1990 | 2170237 |
| interrelationship of blood transfusion, non-a, non-b hepatitis and hepatocellular carcinoma: analysis by detection of antibody to hepatitis c virus. | to clarify the relationship between hepatitis c virus infection and the development of hepatocellular carcinoma as sequelae of non-a, non-b posttransfusion hepatitis, 231 patients with chronic non-a, non-b hepatitis (96 with chronic hepatitis, 81 with cirrhosis and 54 with hepatocellular carcinoma) were analyzed for antibody to hepatitis c virus and were compared with 125 patients with chronic hepatitis b (50 with chronic hepatitis, 46 with cirrhosis and 29 with hepatocellular carcinoma). antibo ... | 1990 | 2170265 |
| isolation and purification of a non-a, non-b hepatitis-associated microtubular aggregates protein. | blood-borne type non-a, non-b (nanb) hepatitis-associated microtubular aggregates protein was isolated and partially sequenced. the microtubular aggregates were isolated from the hepatocytes of nanb-infected chimpanzees and were found to have a buoyant density in sucrose solution of 1.21 to 1.23 g/ml. a single protein, recognized by our anti-microtubular aggregates monoclonal antibodies, was found to have an mr of 44,000 (p44). this p44 protein was not found in uninfected chimpanzees. we determi ... | 1990 | 2170569 |
| cloning, sequencing and expression in escherichia coli of cdna for a non-a, non-b hepatitis-associated microtubular aggregates protein. | a 1.7 kb cdna encoding a novel antigen (p44; apparent mr 44k) associated with non-a, non-b (nanb) hepatitis, was isolated from the hepatic cdna library of a chimpanzee infected with nanb hepatitis. the library was screened with a monoclonal antibody against this antigen. the cdna cloned contained an open reading frame encoding a 444 amino acid protein with an mr calculated to be 50,468. the cdna hybridized to a 1.9 kb mrna obtained from chimpanzee hepatocytes infected with either the nanb or hep ... | 1990 | 2170570 |
| risk factors for acute non-a, non-b hepatitis in the united states and association with hepatitis c virus infection. | the centers for disease control conducted intensive surveillance for acute non-a, non-b hepatitis in four sentinel counties over a 7-year period. testing for antibody to hepatitis c virus was performed with the newly developed enzyme immunoassay. the incidence of non-a, non-b hepatitis remained relatively stable (average, 7.1 cases per 100,000, but there were significant changes in disease transmission patterns. the proportion of patients with a history of blood transfusion declined from 17% to ... | 1990 | 2170702 |
| the 5'-terminal sequence of the hepatitis c virus genome. | the 5'-terminal sequence of the genome of hepatitis c virus (hcv) was determined for two distinct hcv strains in human and chimpanzee carriers. it had a 5'-noncoding region of at least 324 nucleotides, well preserved by the two strains with a high homology (99.1%), followed by 1348 nucleotides that continued to the documented sequence of prototype hcv spanning 7310 nucleotides (european patent application #88310922.5). based on these results, hcv is considered to possess an uninterrupted open re ... | 1990 | 2170712 |
| frequency and significance of antibody to hepatitis c virus in severe corticosteroid-treated cryptogenic chronic active hepatitis. | to determine the frequency and significance of antibody to hepatitis c virus (anti-hcv) in severe cryptogenic chronic active hepatitis (cah), we tested sera from 17 corticosteroid-treated patients by an enzyme immunoassay. specificity of the antibodies to hcv-encoded antigens was assessed by recombinant immunoblot assay. the findings in patients with and without anti-hcv were contrasted, and the frequency of seropositivity was compared with that in patients who had other types of chronic liver d ... | 1990 | 2170781 |
| hepatitis c: from laboratory to bedside. | 1990 | 2170783 | |
| evaluation of antibodies to hepatitis c virus in a study of transfusion-associated hepatitis. | the hepatitis c virus (hcv) is now known to be the chief cause of transfusion-associated non-a, non-b hepatitis, but the prevalence of hcv among blood donors and the frequency of transmission by blood transfusion are unknown. | 1990 | 2170839 |
| [the hepatitis c virus--principal causative agent in non-a, non-b hepatitis]. | the diagnosis of non-a, non-b (nanb) hepatitis was based on the exclusion of hepatitis a and b infection and other known causes of hepatitis, such as other viruses, alcohol and toxic effects. more recently, an rna has been isolated from a nanb-infected chimpanzee serum and with its aid a polypeptide antigen was produced by molecular biological methods. antibodies to this antigen--as part of the infectious agent, called hepatitis c virus (hcv)--were detected in the sera of infected patients. stud ... | 1990 | 2170894 |
| antibodies against hepatitis c in a population of swedish haemophiliacs and heterosexual partners. | the prevalence of antibodies against hepatitis c virus in 160 swedish patients with haemophilia or related coagulation disorders and 13 heterosexual partners was assessed by means of an elisa method. a high prevalence of hcv antibodies was found in haemophiliacs with chronic hepatitis (87%), with hiv-antibodies (87%), with severe form of the coagulopathy (81%), and among those who at any point had received factor concentrates without viral inactivation (79%) or of foreign origin (84%). on the ot ... | 1990 | 2171136 |
| early appearance of antibodies to hepatitis c virus in community acquired acute non-a, non-b hepatitis is associated with progression to chronic liver disease. the copenhagen hepatitis acuta programme. | 24 consecutive patients (14 females; median age 36, range 18-77) with liver biopsy proven acute non-a, non-b hepatitis (nanbh) were assayed for antibodies to hepatitis c virus (hcv). 14 (58%) were positive initially or during follow-up. three patients were positive within 4 weeks following onset of symptoms and 7 patients in a serum sample obtained 4-8 weeks after clinical onset. seroconversion was documented in 7/8 patients in paired sera from the acute phase of the disease. anti-hcv was detect ... | 1990 | 2171137 |
| [serum antibodies against hepatitis c: study of 4,100 blood donors from the northeast of france]. | between june 1989 and february 1990 the prevalence of antibodies to hepatitis c virus (anti-hcv) was studied in blood donations from 4,100 donors tested in north east france. 37 samples were found reactive for anti-hcv (prevalence of anti-hcv: 0.90%) without any significant difference in sex (males: 0.94%; females: 0.87%) and age distributions. 178 (4.34%) of the 4,100 donors were found anti-hbc positive, 5 of these donors being anti-hcv positive (13.51% of all anti-hcv positive donors). 52 dono ... | 1990 | 2171543 |
| antibodies to hepatitis c virus in patients with positive hbsag. | 1990 | 2171886 | |
| detection of hepatitis c virus rna in sera and liver tissues of non-a, non-b hepatitis patients using the polymerase chain reaction. | sera obtained from patients with non-a, non-b hepatitis were examined for the presence of hepatitis c virus (hcv) genome by using the reverse transcription-polymerase chain reaction assay, as well as for antibody to hcv (anti-hcv) by using an enzyme-linked immunosorbent assay (elisa). we also examined the presence of hcv rna in liver tissue obtained by surgical resection of hepatocellular carcinoma. among 33 patients, hcv rna was detectable in 21 (64%), and the antibody was also positive in 21 ( ... | 1990 | 2172195 |
| [progress on diagnosis and therapy of hepatitis, a, hepatitis b and hepatitis c]. | 1990 | 2172427 | |
| [clinical significance of hepatitis c virus antibody in diagnosis of hepatitis c]. | 1990 | 2172428 | |
| specificity of the hepatitis c virus antibody elisa in patients with hepatocellular carcinoma. | 1990 | 2172561 | |
| [advances in the research on non-a, non-b hepatitis: hepatitis c virus. multicenter anti-hcv studies]. | in the introduction, a brief overview is given on the clinical aspects and classification of non-a, non-b hepatitis and on the discovery of hepatitis c virus. using a recombinant hepatitis c virus antigen in an elisa test system, for the demonstration of the antibody to hepatitis c virus, a new possibility became available in the serological diagnosis of non-a, non-b hepatitis. according to the previous hepatitis c virus antibody studies performed in western europe and in hungary, the results of ... | 1990 | 2172888 |
| [hepatitis c virus]. | 1990 | 2173037 | |
| prevalence of anti-hcv in norwegian blood donors with anti-hbc or increased alt levels. | testing for hepatitis b core antibodies (anti-hbc) was performed in 12,104 consecutive blood donors, 139 (1.15%) of whom were found to be positive. the first 6036 donors were also screened for alt; 91 (1.51%) had repeatedly elevated values. alt screening was of no help in detecting anti-hbc-positive donors. those with anti-hbc or repeatedly raised alt levels were further tested for hepatitis c virus antibodies (anti-hcv), and 3 (2.16%) and 1 (1.10%) anti-hcv-positive donors were detected, respec ... | 1990 | 2173175 |
| prevalence of anti-hcv antibody in blood donors in the tokyo area. | prospective studies of posttransfusion hepatitis carried out in the past decade showed that 18.1% of the blood transfusions resulted in non-a non-b hepatitis in japan. as an approach to the prevention of posttransfusion non-a non-b hepatitis (ptnanb), anti-hepatitis c virus (hcv) positivity was measured in 2,970 blood donations in the tokyo area, and in 200 children aged between 6 and 15 years. thirty-four cases were anti-hcv-positive, showing an overall positivity of 1.14%. none of the 200 chil ... | 1990 | 2173281 |
| detection, semiquantitation, and genetic variation in hepatitis c virus sequences amplified from the plasma of blood donors with elevated alanine aminotransferase. | hepatitis c virus (hcv) is the predominant etiologic agent of posttransfusion non-a, non-b hepatitis, characterized by undulating elevation of alanine aminotransferase (alt) and chronic liver disease. a commercial enzyme-linked immunosorbent assay detected antibodies to hcv (anti-hcv) in 11 specimens among 101 nontransfusable plasma units obtained from asymptomatic, volunteer blood donors with elevated levels' of alt. using a combined reverse-transcription polymerase chain reaction (rt-pcr) assa ... | 1990 | 2173725 |
| detection of hepatitis c virus ribonucleic acid in the serum by amplification with polymerase chain reaction. | hepatitis c virus (hcv) rna was detected in the sera of patients with non-a, non-b chronic liver disease by polymerase chain reaction (pcr). rna was extracted from the serum, reverse transcribed to cdna, and amplified by pcr. with this method, 30 patients with non-a, non-b chronic liver disease and 10 healthy subjects were tested. hcv rna was detected in 13 of 16 (81%) anti-hcv-positive patients and also in 7 of 14 (50%) anti-hcv-negative patients, but in none of 10 anti-hcv-negative healthy sub ... | 1990 | 2173727 |
| hepatitis non-a, non-b viruses become identified as hepatitis c and e viruses. | 1990 | 2173845 | |
| ["non-a-non-b" viruses have been identified]. | 1990 | 2174156 | |
| [discovery of the hepatitis c virus]. | after 15 years of unsuccessful attempts, the most frequent of non-a non-b (nanb) hepatitis viruses has recently been identified and designated hcv. it is by an original and direct molecular biology approach, leading to the cloning of nucleic acids presumed to be present in an infectious plasma, that this virus could be partially characterized. the viral genome was sequenced before the agent could be detected by serology or electron microscopy. hcv is a small rna virus with a lipid capsid, and it ... | 1990 | 2174157 |
| [has the hepatitis c virus been discovered?]. | 1990 | 2174481 | |
| hepatitis c viral sequences in sera during the acute phase of infection. | 1990 | 2174649 | |
| sexual transmission of hepatitis c virus and its relation with hepatitis b virus and hiv. | to determine the extent of transmission of hepatitis c virus in sexual partners of intravenous drug misusers and to examine the relation between the prevalences of hiv, hepatitis b virus, and hepatitis c virus infections in homosexual men and intravenous drug misusers and their sexual partners. | 1990 | 2174705 |
| antibodies to hepatitis c virus in blood donors. | recently a recombinant polypeptide of hepatitis c virus (hcv) has been developed by the chiron corporation in california. this antigen has been used to develop an elisa test (ortho diagnostic systems for serum anti-hcv antibodies. preliminary data have shown that this virus is the major cause of nanb hepatitis in the world. we examined differences in anti-hcv prevalence among subgroups of blood donors (total sera examined 639) classified for past or present exposure to hbv or not, and for alt le ... | 1990 | 2174795 |
| application of a numerical scoring system for assessment of histological outcome in patients with chronic posttransfusion non-a, non-b hepatitis with or without antibodies to hepatitis c. | a numerical scoring system was applied and compared to the conventional histological classification to assess the histological status of liver specimens from 37 patients with chronic posttransfusion non-a, non-b hepatitis followed for 7 to 105 months (mean 35 months). four histological categories of alterations were assessed and scored: piecemeal necrosis (pmn), fibrosis and cirrhosis, lobular necrosis and portal inflammation. sequential liver biopsies were obtained from 19 patients. pmn was gen ... | 1990 | 2175005 |
| kidney transplants with positive anti-hepatitis c virus donors. | 1990 | 2175058 | |
| [a comparative study of non-a, non-b hepatitis with a fecal-oral mechanism of the transmission of the causative agent in the ussr and india]. | 1990 | 2175067 | |
| [virus c]. | 1990 | 2175159 | |
| [hepatitis c in patients with transplanted organs]. | 1990 | 2175161 | |
| hepatitis c virus antibodies in chronic alcoholic patients: association with severity of liver injury. | the prevalence of hepatitis c virus antibody and its relationship to the severity of liver disease in chronic alcoholic patients has been assessed, using a recently developed enzyme immunoassay and confirmed by a recombinant immunoblot assay, in 144 patients (mean age +/- s.d. = 44.4 +/- 11.3 yr) who had consumed greater than 80 gm/day ethanol for greater than 5 yr. hepatic disease was evaluated by clinical and biochemical studies and by liver biopsy when appropriate. in addition, 76 liver biops ... | 1990 | 2175291 |
| association of autoimmune hepatitis with hla-bw54 and dr4 in japanese patients. | human leukocyte antigen-d region-related alleles (human leukocyte antigen dr and dq) and human leukocyte antigen class i alleles were typed serologically in 31 japanese patients with autoimmune hepatitis. these patients had increased serum levels of ast and igg, high titers of autoantibodies, no history of blood transfusion and were negative for hbsag and antibodies to hbc. three hundred eighty-six healthy subjects and 30 patients with cryptogenic chronic hepatitis served as control groups. the ... | 1990 | 2175292 |
| low frequency of hepatitis c antibodies among children from foreign countries adopted in swedish families. | 89 children from 20 countries adopted to swedish parents between 1984 and 1988 were tested for the presence of hepatitis c antibodies (anti-hcv). sera taken soon after their arrival to sweden were retrospectively analyzed. two children, an 18-month-old boy from poland and an 8-month-old girl from india, were anti-hcv positive. the girl from india had lost the anti-hcv antibodies in sera taken at the age of 13 and 14 months. these antibodies were probably passively derived from the mother. the po ... | 1990 | 2175449 |
| hepatitis c virus antibody in acute and chronic liver diseases in india. | 1990 | 2175450 | |
| [an epidemiological investigation on an outbreak of non-a, non-b hepatitis occurring after a dinner party]. | this paper reports an investigation on an outbreak of non-a, non-b hepatitis occurred after a dinner party. the attack rate was 25.89%. the ratio of male to female was 1.48 to 1. two patients were suffering from fulminant hepatitis. the attack rates showed no significant difference among various age groups. thirty-three percent of the patients had jaundice and 50% had fever. the results indicated that this outbreak was probably related to some food taken during the party. | 1990 | 2175674 |
| new blood safety tests. ottawa. | 1990 | 2175725 | |
| finally ... the hepatitis c virus. | 1990 | 2175759 | |
| molecular cloning of the human hepatitis c virus genome from japanese patients with non-a, non-b hepatitis. | the nucleotide sequence of the japanese type of hepatitis c virus (hcv-j) genome, consisting of 9413 nucleotides, was determined by analyses of cdna clones from plasma specimens from japanese patients with chronic hepatitis. hcv-j genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. comparison of hcv-j with the american isolate of hcv showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. thus hcv-j and the america ... | 1990 | 2175903 |
| transition of antibody to hepatitis c virus from chronic hepatitis to hepatocellular carcinoma. | fifty-eight patients with chronic hepatitis c were followed for more than 7 years. of them, 10 patients were found to develop hepatocellular carcinoma, 14 to develop liver cirrhosis, 30 to sustain chronic hepatitis, and 4 to show subsidence of hepatitis. antibody to hepatitis c virus (anti-hcv) disappeared from the 4 patients whose hepatitis subsided, but it persisted in the remaining 54 patients. the mean titer of anti-hcv was almost the same at the stages of chronic hepatitis and of cancer in ... | 1990 | 2176199 |
| a structural protein encoded by the 5' region of the hepatitis c virus genome efficiently detects viral infection. | a structural region of the hepatitis c virus genome was molecularly cloned. a protein expressed in vitro by transcription followed by translation was precipitated immunologically by sera from patients with chronic hepatitis or hepatocellular carcinoma that were positive for antibody against the non-structural protein, c100, of hepatitis c virus, but not by sera from healthy persons. thus, this structural protein should be useful for detection of infection with this virus. | 1990 | 2176200 |
| hepatitis c virus antibodies in subjects with and without liver disease in the united kingdom. | the prevalence of antibody to hepatitis c virus, evidence of previous or current infection with this agent of parenterally transmitted non-a, non-b hepatitis, was determined in 340 subjects residing in the united kingdom. the antibody was detected in 3 per cent of unselected blood donors and in 60 per cent of patients with chronic post-transfusion non-a, non-b hepatitis. evidence for infection was also found in 30 per cent of intravenous drug abusers, and in 75 per cent of haemophiliacs receivin ... | 1990 | 2176297 |
| antibody to hepatitis c virus after a vapour-heated factor viii concentrate. the study group of the fondazione dell'emofilia. | to evaluate whether or not clotting factor concentrates exposed to virucidal procedures transmitted hepatitis c, sera obtained in 1984-1986 from 27 previously untreated hemophiliacs infused with a vapour-heated factor viii concentrate were tested retrospectively for the antibody to the hepatitis c virus (anti-hcv). a 2-year-old hemophiliac, negative for anti-hcv before administration of concentrate, seroconverted at week 12 and remained anti-hcv positive thereafter. both his parents were anti-hc ... | 1990 | 2176748 |
| the putative nucleocapsid and envelope protein genes of hepatitis c virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers. | cdna fragments of a 5'-terminal region of the hepatitis c virus (hcv) genome were isolated by the reverse polymerase chain reaction from rna extracted from plasma samples of healthy japanese carriers. their nucleotide sequence was compared with that of the original isolate which had been passaged twice in chimpanzees. no deletions or insertions were observed between the two sequences in the regions examined. both the 5' untranslated and putative nucleocapsid (core) protein regions were highly co ... | 1990 | 2177094 |
| the significance of anti-hepatitis c virus antibodies measured in chronic liver disease. | the frequency of hepatitic c virus (hcv) antibodies was determined in two different laboratories in stored sera from 128 consecutive patients with chronic liver disease and from 41 healthy blood donors. repeated measurements were performed in most patients. at the first determination the frequency of hcv antibodies was 7% in primary sclerosing cholangitis, 42% in primary biliary cirrhosis, 40% in autoimmune chronic active hepatitis, and 27% in alcoholic liver disease. the reproducibility of the ... | 1990 | 2177219 |
| autoimmune chronic active hepatitis: changing reactivity for antibodies to hepatitis c virus after immunosuppressive treatment. | thirty-three patients fulfilling stringent criteria for autoimmune chronic active hepatitis (cah) were tested for antibodies against hepatitis c virus (anti-hcv) with an enzyme immunoassay. eleven of the 33 (33%) patients were anti-hcv-reactive in a serum drawn before initiation of immunosuppressive therapy. anti-hcv-reactive patients had significantly higher median s-igg level (32.3 g/l) than nonreactive patients (23.5 g/l) (p less than 0.01). when in remission with normalized s-igg levels as a ... | 1990 | 2177220 |
| [non-a, non-b hepatitis: hepatitis c]. | non-a, non-b hepatitis (nanb), whether following the transfusion of blood products or occurring sporadically without percutaneous exposure, is complicated in approximately 50% of cases by the development of chronic hepatitis. emerging as a consistent observation is the insidious progression of such cases of chronic nanb hepatitis to cirrhosis. among patients with chronic nanb hepatitis followed for up to 10 years, cirrhosis is demonstrable in 20%. in may, 1988, the identification of the agent of ... | 1990 | 2177314 |