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structural proteins of western equine encephalitis virus: amino acid compositions and n-terminal sequences.the structural proteins of western equine encephalitis virus, a member of the alphavirus group, have been characterized by the determination of their amino acid compositions and by n-terminal sequence analysis. more than 60 residues of the n-terminal sequences of each of the envelope glycoproteins have been determined. a comparison of these sequences with the previously determined sequences of two related alphaviruses. sindbis virus and semliki forest virus, strongly supports the view that all t ...19836834471
a variant of western equine encephalitis virus with nonglycosylated e3 protein.a variant clone (at/a125) of western equine encephalitis virus was isolated from a line of mosquito cells persistently infected with a temperature-sensitive parent strain, a125. variant-infected cells produced an altered form of pe2 protein which migrated with a higher electrophoretic mobility than wild type or a125 pe2. the altered pe2, like pe2 of wild type, was precipitated by anti-envelope proteins serum but not by anti-e1 serum. in pulse-chase experiments the altered pe2 protein was shown t ...19836829167
adjuvant activity of a novel metabolizable lipid emulsion with inactivated viral vaccines.studies were conducted in mice, hamsters, sheep, and two species of nonhuman primates which demonstrate the adjuvant activity of a new metabolizable lipid emulsion with marginally immunogenic doses of formalin-inactivated viral vaccines. the lipid base consists of highly refined peanut oil emulsified in aqueous vaccines with glycerol and lecithin. hamsters and mice inoculated with lipid emulsion plus western or venezuelan equine encephalitis vaccine were significantly more resistant than vaccina ...19806772571
lateralized eeg abnormalities in western equine encephalitis. 19846744162
focal neurologic signs in western equine encephalitis.focal neurologic signs developed in a 75-year-old woman who had presented with encephalitis. a diagnosis of western equine encephalitis was made from serologic findings. although she was improving clinically the patient died from acute dissection of an aortic aneurysm 11 days after admission to hospital. the pathological findings--perivascular infiltrates and multifocal necrosis in the deep grey matter--were consistent with the diagnosis of western equine encephalitis. similar findings in the ba ...19846704848
leads from the mmwr. arboviral encephalitides--united states, 1983. 19836644947
effect of temperature of extrinsic incubation on the vector competence of culex tarsalis for western equine encephalomyelitis virus.culex tarsalis was a less competent vector of western equine encephalomyelitis (wee) virus after 2-3 weeks' extrinsic incubation at 32 degrees c than after incubation at 18 degrees or 25 degrees c. the high temperature itself was not directly detrimental to mosquito infection as all mosquitoes were initially infected, but subsequently some females were able to limit viral multiplication and/or dissemination. elevated maintenance temperatures enhanced the expression of modulation, and elevated la ...19836625067
serodiagnosis of western equine encephalitis virus infections: relationships of antibody titer and test to observed onset of clinical illness.sera from horses and human beings with clinically diagnosed western equine encephalitis (wee) virus infections were tested for hemagglutination-inhibition (hi), complement-fixation (cf), and neutralizing (n) antibody to wee virus. these tests confirmed infection in 43.8% (hi), 56.3% (cf), and 80.4% (n) of horses and 54.5% (hi), 59.1% (cf), and 77.3% (n) of human beings. use of the n test as an adjunct to the hi and cf tests increased the likelihood of serologic confirmation to 91.7%. in both hor ...19836618970
arthropod-borne virus infections of the central nervous system.arthropod-borne viruses (arboviruses) are an important cause of meningoencephalitis in north america. this article considers the common features of the four major cns arbovirus infections in this country and gives detailed descriptions of the individual virus infections.19846503938
arboviral encephalitides--united states, 1983. 19836415386
opsonization of alphaviruses in hamsters.immune elimination of alphaviruses in immunized hamsters appears to involve formation of virus/antibody aggregates which are subsequently cleared from the circulation by cells of the reticuloendothelial system (res). virulent strains of venezuelan (vee) and western equine encephalitis (wee) viruses which were cleared slowly from the circulation of nonimmune hamsters, were cleared rapidly when inoculated into the blood of immunized hamsters. likewise, when these viruses were mixed with specific h ...19836311964
comparative arboviral susceptibility of female culex tarsalis (diptera: culicidae) collected in co2-baited traps and reared from field-collected pupae. 19836298431
arboviruses from western utah, usa, 1967-1976. 19836135808
permeability of the midgut basal lamina in the mosquito, culex tarsalis coquillett (insecta, diptera).the permeability of the basal lamina of the midgut of the mosquito, culex tarsalis, was determined for engorged and unfed mosquito midguts both pre- and postfixed. a range of materials, with diameters from less than 2nm (lanthanum) through 90 nm, (polystyrene spheres), was examined. the permeability of the basal lamina was constant under all experimental conditions; 5--8 nm particles (colloidal thorium) were the largest to consistently permeate. the discussion is centered on the question of how ...19816115555
nucleoside triphosphate phosphohydrolase produced in bhk cells infected with western equine encephalitis virus is probably associated with the 82k dalton nonstructural polypeptide. 19816115512
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 50-1984. a 53-year-old man with fever and rapid neurologic deterioration. 19846095078
culex tarsalis coquillett as a vector of an attenuated strain of western equine encephalomyelitis virus. 19665933958
western equine encephalitis virus in saskatchewan garter snakes and leopard frogs.western equine encephalitis virus was isolated from two naturally infected snakes on first bleeding and from seven others at subsequent bleedings, both with and without preliminary chilling. one snake, with neither detectable virus nor serum neutralizing antibodies when first bled, developed viremia later. viremia in garter snakes has a cyclic rhythm independent of the temperature of the environment. virus was isolated from 6 frogs, and 50 out of 179 had detectable serum neutralizing antibodies. ...19665919753
serologic evidence of california encephalitis virus and western equine encephalitis virus in snowshoe hares. 19645895901
influence of heavy water (deuterium oxide) on plaque size of western equine encephalitis virus. 19655895039
[variability of the western equine encephalomyelitis virus during the process of passage through several organisms]. 19655894662
attempts to induce recomibinants somatic cells in chicken erythrocyte chimaeras by repeated injection of western equine encephalomyelitis virus. 19695815870
isolation of western equine encephalitis virus from an opossum.the first isolation of an arbovirus (western equine encephalo-myelitis virus) from an opossum in the united states is reported.19695763879
replication of western equine encephalomyelitis virus. ii. cytoplasmic structure involved in the synthesis and development of the virions.analysis of the cytoplasmic fraction of chick embryo cells during the exponential phase of western equine encephalomyelitis (wee) virus growth showed that the viral ribonucleic acid (rna) labeled by a short pulse with (3)h-uridine was associated with a structure which sedimented in sucrose density gradients with a coefficient of 65s. the rna extracted from this structure sedimented in sucrose density gradients at 26s. after a longer period of exposure to (3)h-uridine, the radio-active viral rna ...19685723707
replication of western equine encephalomyelitis virus. i. some chemical and physical characteristics of viral ribonucleic acid.the ribonucleic acid (rna) from western equine encephalomyelitis (wee) virions sedimented through sucrose gradients with a sedimentation coefficient of 40s. another viral rna which was always associated with infected cells possessed a sedimentation coefficient of 26s. both 40s and 26s rna had identical base compositions and densities. the 40s rna displayed a hyperchromic effect when heated with a t(m) of 57.5 c. when 40s rna was heated at 90 c and cooled rapidly, it sedimented with a coefficient ...19685697470
preparation of a stable non-infective western equine encephalomyelitis virus antigen from allantoic fluid. 19685693066
suppressed penicillin toxicity in immunized guinea pigs surviving challenge with western equine encephalitis virus. 19685640390
nonspecific arbovirus hemogglutinin inhibitors in turtle sera. 19715574411
interfering factor in chicken tissues infected with infectious bursal agent against western equine encephalomyelitis virus. 19705453402
[the effect of beta-propiolactone and heat on the antigenic properties of a series of arboviruses]. 19695407009
isolation of two plaque mutants of western equine encephalitis virus differing in virulence for mice.two plaque mutants were isolated from tissue cultures infected persistently with western equine encephalitis virus. a large plaque mutant proved to be markedly avirulent for mice.19695360528
absence of an all-or-none type of complement requirement in early neutralizing antibody against western equine encephalitis virus. 19715313614
[cultivation of various arboviruses in new transplantable lines of mouse fibroblasts]. 19715168877
wild birds as eastern (eee) and western (wee) equine encephalitis sentinels. 19715156487
cross-protection in hamsters immunized with group a arbovirus vaccines.cross-protection between venezuelan, eastern, and western equine encephalomyelitis (vee, eee, wee) viruses was studied in the hamster by using challenge responses and neutralizing antibody titers as indexes of protection. formalin-inactivated vaccines induced only homologous protection regardless of the sequence of vaccination or the combination of vaccines employed. use of attenuated vee vaccine, singly, produced absolute homologous protection as well as 37 and 59% protection against wee and ee ...19715154876
relationship between neurovirulence and temperature sensitivity of an attenuated western equine encephalitis virus. 19715137591
trinity river serologic survey. a survey of residents of communities along the course of the trinity river between houston and dallas, texas, for antibodies to the viruses of st. louis encephalitis, western equine encephalitis and california encephalitis. 19715120543
neurologic, intellectual, and psychologic sequelae following western encephalitis. a follow-up study of 35 cases. 19715106260
inactivated western equine encephalomyelitis vaccine propagated in chick embryo cell culture. clinical and serological evaluation in man. 19715105896
interferon assays and quantitative virus determinations in a fatal infection in man with western equine encephalomyelitis virus. 19715093674
early protection in hamsters immunized with attenuated venezuelan equine encephalomyelitis vaccine.the rapid onset and persistence of homologous and heterologous protection induced by attenuated venezuelan equine encephalomyelitis (vee) vaccine (tc-83) were studied in the hamster, by using challenge response as the index of protection. at 8 hr postvaccination with 10(3) median immunizing doses of tc-83 vaccine, 15 to 20% of animals were protected against challenge with vee virus as well as eastern and western equine encephalomyelitis viruses. the percentage of protection increased with time p ...19725086903
[combined use of interferon and various styrylquinolines in cell cultures and experimental arbovirus infections]. 19725086860
virulence of a temperature-sensitive mutant of western equine encephalitis virus. 19725083407
radioimmune precipitation of group a arboviruses. 19725066343
inhibitors of western and venezuelan equine encephalitis viruses in cattle sera from hawaii. 19725061277
mouse potency assay for western equine encephalomyelitis vaccines.a potency assay for western equine encephalomyelitis vaccine was developed which utilized mice as the test animal instead of guinea pigs or hamsters. by immunizing several groups of mice with dilutions of the vaccine and challenging them intracerebrally with virulent virus, it was possible to determine mathematically a dose of vaccine capable of protecting 50% of the animals (ed(50)). when log dilutions of virulent virus were used to challenge mice which were immunized with dilutions of the vacc ...19725059614
recurrent st. louis encephalitis infection in residents of a flood plain of the trinity river, roosevelt heights (dallas, texas). 19725048980
arbovirus infections in man in british columbia.during the summer of 1971, the first laboratory-proved cases of acute encephalitis in man due to any of the known arboviruses occurred in the south-central region of british columbia. five human cases of encephalitis with two deaths were diagnosed; three of these patients, including one of the fatalities, were proven in the laboratory to have contracted western equine encephalitis.during 1968 and 1969, a human serum survey undertaken in approximately 2000 life-long residents of the province disc ...19725034698
arboviruses west and north. 19725034693
the pathogenesis of western equine encephalitis virus (w.e.e.) in adult hamsters with special reference to the long and short term effects on the c.n.s. of the attenuated clone 15 variant. 19725014245
experimental infection of bobwhite quail (colinus virginianus) with western equine encephalitis (wee) virus. 19725007901
immunosuppression: a means to assess the role of the immune response in acute virus infections. 19715001450
mechanism of enhancement of newcastle disease virus growth in cultured cells by co-infecting hog cholera virus. 19715000803
cytoplasmic localization of ribonucleic acid synthesis induced in the cells infected with some group a arboviruses. 19714997624
an attenuated strain of western equine encephalitis virus as a possible live immunizing agent. 19644955698
survival of western equine encephalitis virus in triatominae. 19684880889
virus inhibitory activity of some pyrimidine derivatives and trimethinium salts in tissue culture. 19744859585
enhanced detection of western equine encephalitis virus plaque variants in crowded cultures and plaque progeny--potential use in genetic studies.enhanced detection of large-plaque variants of western equine encephalitis virus in the presence of large numbers of small-plaque western encephalitis virus was achieved by using higher bicarbonate concentrations in the agar-overlay medium and increasing concentrations of co(2) in an enclosed environment during the time plaques were developed with neutral red. by this technique we were able to detect large-plaque mutants in cloned small-plaque suspensions for the first time and to show an increa ...19744858504
rapid identification of western equine encephalomyelitis virus. 19744856671
comparative electrophoretic studies on the structural proteins of selected group a arboviruses. 19744841596
proceedings: mathematical modeling of a virus vector: culex tarsalis. 19744824238
clinical studies of an attenuated virus cell culture venezuelan equine encephalomyelitis vaccine. 19734803525
[nonspecific resistance of tissue cultures, grown in a medium with serum lipid inhibitors, to arboviruses]. 19734797701
[study of the multiplication of tiuleniĭ, sakhalin and baku arboviruses in cell cultures]. 19734789979
[a cryomethod of concentrating antibodies]. 19734789252
serologic examination of some domestic animals from south moravia on the presence of antibodies to selected arboviruses of the a, b, california and bunyamwera groups. 19734777760
possible evidence for interference with venezuelan equine encephalitis virus vaccination of equines by pre-existing antibody to eastern or western equine encephalitis virus, or both.during 1971, an epizootic of venezuelan equine encephalitis (vee) reached the united states. laboratory tests were performed on a large number of sick, healthy, unvaccinated, and vaccinated horses. neutralization (n) tests in cell cultures revealed that 153 of 193 (79.3%) equines outside the state of texas and 175 of 204 (85.8%) within texas (82.6% overall) had detectable n antibody to vee virus a week or more after vaccination. twenty-six of 40 (65%) non-texas equines and 18 of 29 (62%) texas e ...19734751797
california encephalitis studies in iowa during 1969, 1970 and 1971. 19734735659
"zaliv terpeniya" virus, a new uukuniemi group arbovirus isolated from ixodes (ceratixodes) putus pick.-camb. 1878 on tyuleniy island (sakhalin region) and commodore islands (kamchatsk region). 19734727778
host defenses during primary venezuelan equine encephalomyelitis virus infection in mice. ii. in vitro methods for the measurement and qualitation of the immune response. 19734697832
surveillance of arboviral encephalitis in the united states, 1955-1971. 19734692993
ecology of western encephalitis virus. 19734692002
house sparrows, passer domesticus (l.), as hosts of arboviruses in hale county, texas. ii. laboratory studies with western equine encephalitis virus. 19734688421
house sparrows, passer domesticus (l.), as hosts of arboviruses in hale county, texas. i. field studies, 1965-1969. 19734688420
considerations on the ecology of several arboviruses in eastern long island. 19734684881
[low temperature thresholds of reproduction of group a arboviruses in tissue culture]. 19724672889
specific membranous structures associated with the replication of group a arboviruses.intracytoplasmic membranous structures of a unique type were associated with the replication of three group a arboviruses: semliki forest virus (sfv), sindbis virus, or western equine encephalomyelitis virus. the structures, referred to as type 1 cytopathic vacuoles (cpv-1), were membrane-limited and characteristically lined by regular membranous spherules measuring 50 nm in diameter. the membranous spherules typically contained a fine central density, but were neither virus cores nor virions. d ...19724672392
elimination of repeated clot formation in mouse ascitic fluid containing arbovirus antibodies.repeated clot formation in mouse ascitic fluids containing antiviral antibody was eliminated by acid precipitation of the fibrinogen.19724672320
susceptibility of the mongolian gerbil (meriones unguiculatus) to western equine encephalitis. 19724556102
japanese quail embryo cells as a host of group a arboviruses. 19734541936
a comparison of clinical manifestations and pathology of the equine encephalidites: vee, wee, eee. 19734534742
neutralizing antibody against akabane virus in precolostral sera from calves with congenital arthrogryposis-hydranencephaly syndrome. 19744451432
experimental infection with western equine encephalomyelitis virus in wild rodents indigenous to kern county, california.six species of rodents from kern county, california, were inoculated subcutaneously with western equine encephalomyelitis (wee) virus to determine their susceptibility to infection and their potential as natural hosts. ammospermophilus nelsoni, citellus beecheyi, dipodomys heermanni, dipodomys nitratoides, peromyscus maniculatus, and sciurus griseus were readily infected. infection was usually fatal in dipodomys species, c. beecheyi, and s. griseus, but was clinically inapparent in other species ...19744426699
immune response of horses after simultaneous or sequential vaccination against eastern, western, and venezuelan equine encephalomyelitis. 19744422323
the influence of cell-free extracts from ehrlich ascitic carcinoma and rous sarcoma on the action of antiviral inhibitors. 19724404902
[detection of hemagglutination-inhibiting antibodies to arboviruses in sera of young individuals in the federal republic of germany and in the republic of togo (west africa)]. 19714403535
ecology of arboviruses in a maryland freshwater swamp. 3. vertebrate hosts. 19724403123
ecology of arboviruses in a maryland freshwater swamp. i. population dynamics and habitat distribution of potential mosquito vectors. 19724403121
studies on arbovirus haemagglutinin: differential inhibition of haemagglutinin formation by 6-azauridine and uridine kinase-containing cell-free extract. 19724402254
agents encountered during arboviral ecological studies: tampa bay area, florida, 1963 to 1970. 19724400812
isolation of tick-borne encephalitis virus from the brain of a sick dog in switzerland. 19724400683
on the presence of viruses of the american equine encephalomyelitis in central europe. review. 19714399101
arboviruses isolated from mosquitoes collected in southeastern iowa in 1966. 19714398432
inhibition of virus multiplication by selected isothiocyanates. 19714398087
inhibition of virus multiplication by some derivatives of pyridine- and pyrazinecarboxylic acid. 19714398086
variability of western equine encephalomyelitis virus. ii. pathogenicity of apathogenic and pathogenic virus variants. 19674382130
variability of western equine encephalomyelitis virus. i. genetic markers of virus variants obtained by formaldehyde treatment and ultraviolet irradiation. 19674382128
experimental cyclic transmission of western equine encephalitis virus in chickens and culex tarsalis through a year. 19674381367
attempts to induce immunological tolerance to western equine encephalomyelitis virus. 19664380775
use of frozen chick and duck embryo cells for plaque assays of arboviruses and rickettsiae. 19744376554
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