Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| a survey of mosquito-borne and insect-specific viruses in hospitals and livestock markets in western kenya. | aedes aegypti and culex pipiens complex mosquitoes are prolific vectors of arboviruses that are a global threat to human and animal health. increased globalization and ease of travel have facilitated the worldwide dissemination of these mosquitoes and the viruses they transmit. to assess disease risk, we determined the frequency of arboviruses in western kenyan counties bordering an area of high arboviral activity. in addition to pathogenic viruses, insect-specific flaviviruses (isfs), some of w ... | 2021 | 34048473 |
| targeting lentiviral vectors to primordial germ cells (pgcs): an efficient strategy for generating transgenic chickens. | recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. however, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. in this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells (pgcs) to generate transgenic chickens. the lentiviral vectors were pseudotype ... | 2020 | 32274905 |
| arboviruses and blood meal sources in zoophilic mosquitoes at human-wildlife interfaces in kenya. | background: zoophilic mosquitoes play an important role in the transmission of arboviruses of medical importance at human-wildlife interfaces, yet arbovirus surveillance efforts have been focused mostly on anthropophilic mosquitoes. understanding the diversity of zoophilic mosquitoes and their associated feeding patterns and arboviruses can inform better vector control strategies. materials and methods: we morphologically identified mosquitoes collected from two game reserves in kenya, the maasa ... | 2020 | 32155389 |
| infection of western gray kangaroos (macropus fuliginosus) with australian arboviruses associated with human infection. | more than 75 arboviruses (arthropod-borne viruses) have been identified in australia. while alfuy virus (alfv), barmah forest virus (bfv), edge hill virus (ehv), kokobera virus (kokv), murray valley encephalitis virus (mvev), sindbis virus (sinv), ross river virus (rrv), stratford virus (strv), and west nile virus strain kunjin (kunv) have been associated with human infection, there remains a paucity of data regarding their respective transmission cycles and any potential nonhuman vertebrate hos ... | 2020 | 31556842 |
| common vole (microtus arvalis) and bank vole (myodes glareolus) derived permanent cell lines differ in their susceptibility and replication kinetics of animal and zoonotic viruses. | pathogenesis and reservoir host adaptation of animal and zoonotic viruses are poorly understood due to missing adequate cell culture and animal models. the bank vole (myodes glareolus) and common vole (microtus arvalis) serve as hosts for a variety of zoonotic pathogens. for a better understanding of virus association to a putative animal host, we generated two novel cell lines from bank voles of different evolutionary lineages and two common vole cell lines and assayed their susceptibility, rep ... | 2019 | 31513859 |
| activation of rnase l in egyptian rousette bat-derived roni/7 cells is dependent primarily on oas3 and independent of mavs signaling. | bats are reservoirs for many rna viruses that are highly pathogenic in humans yet relatively apathogenic in the natural host. it has been suggested that differences in innate immunity are responsible. the antiviral oas-rnase l pathway is well characterized in humans, but there is little known about its activation and antiviral activity in bats. during infection, oass, upon sensing double-stranded rna (dsrna), produce 2'-5' oligoadenylates (2-5a), leading to activation of rnase l which degrades v ... | 2019 | 31719180 |
| sindbis virus- a wild bird associated zoonotic arbovirus circulates in germany. | sindbis virus (sinv) is an arbovirus causing clinical symptoms such as arthritis, rash and fever following human infections in fennoscandia. its transmission cycle involves mosquito species as vectors as well as wild birds that act as natural reservoir hosts. in germany, sinv was first time observed in 2009 in different mosquito species in the upper rhine valley and one year later in a hooded crow in berlin. recently, sinv was also detected repeatedly at various locations in germany in the conte ... | 2019 | 31767092 |
| host-feeding patterns of culex mosquitoes in iran. | different mosquito-borne pathogens are circulating in iran including sindbis virus, west nile virus, filarioid worms and malaria parasites. however, the local transmission cycles of these pathogenic agents are poorly understood, especially because ecological data on vector species are scarce and there is limited knowledge about the host range; this understanding could help to direct species-specific vector control measurements or to prioritize research. | 2018 | 30587194 |
| linkages between chiropteran diversity and ecosystem services for sustainable fragmented forest conservation. | this data article informs about chiropteran diversity, new records, ecosystem services and possible pathogen carriers in fragmented forests (sub-divided by utility corridors, man-made structures, untouched and secondary plantations) within districts setiu (setiu research station), hulu terengganu (saok and lasir waterfalls) and besut (gunung tebu forest reserve) of state terengganu, peninsular malaysia. these bats were captured using harp traps and mist nets that were set 10 m apart across flywa ... | 2018 | 30533456 |
| arboviruses isolated from the barkedji mosquito-based surveillance system, 2012-2013. | a mosquito-based arbovirus surveillance system was set up at barkedji, senegal after the first outbreak of rift valley fever in west africa in 1988. this system was recently updated using more sampling methods and collecting in greater number of ponds and villages sites. | 2018 | 30541472 |
| imaging of viral neuroinvasion in the zebrafish reveals that sindbis and chikungunya viruses favour different entry routes. | alphaviruses, such as chikungunya virus (chikv) and sindbis virus (sinv), are vector-borne pathogens that cause acute illnesses in humans and are sometimes associated with neuropathies, especially in infants and elderly patients. little is known about their mechanism of entry into the central nervous system (cns), even for sinv, which has been used extensively as a model for viral encephalopathies. we previously established a chikv infection model in the optically transparent zebrafish larva; he ... | 2017 | 28483796 |
| identification of a newly isolated getah virus in the china-laos border, china. | in this study, we isolated a virus strain (yn12031) from specimens of armigeres subalbatus collected in the china-laos border. bhk-21 cells infected with yn12031 exhibited an evident cytopathic effect (cpe) 32 h post-infection. the virus particles were spherical, 70 nm in diameter, and enveloped; they also featured surface fibers. molecular genetic analysis revealed that yn12031 was closely related to alpha viruses such as chikungunya virus and sindbis virus, and located in the same clade as mm2 ... | 2017 | 28427491 |
| biological safety of a highly purified 10% liquid intravenous immunoglobulin preparation from human plasma. | a highly purified 10% liquid intravenous immunoglobulin, iqymune(®), has been developed using an innovative manufacturing process including an affinity chromatography step for the removal of anti-a and anti-b hemagglutinins. | 2017 | 28508264 |
| partially uncleaved alphavirus replicase forms spherule structures in the presence and absence of rna template. | alphaviruses are positive-strand rna viruses expressing their replicase as a polyprotein, p1234, which is cleaved to four final products, nonstructural proteins nsp1 to nsp4. the replicase proteins together with viral rna and host factors form membrane invaginations termed spherules, which act as the replication complexes producing progeny rnas. we have previously shown that the wild-type alphavirus replicase requires a functional rna template and active polymerase to generate spherule structure ... | 2017 | 28701392 |
| application of alphaviral vectors for immunomodulation in cancer therapy. | the lack of specific and efficient cancer therapies has influenced the development of novel approaches, such as immunotherapy, which from its original application of immunogenic protein delivery has developed into the use of more sophisticated recombinant gene delivery methods to achieve better safety and efficacy profiles. this approach involves viral and non-viral delivery systems. | 2017 | 28641531 |
| h-ras exerts opposing effects on type i interferon responses depending on its activation status. | using shrna high-throughput screening, we identified h-ras as a regulator of antiviral activity, whose depletion could enhance sindbis virus replication. further analyses indicated that depletion of h-ras results in a robust increase in vesicular stomatitis virus infection and a decrease in sendai virus (sev)-induced retinoic acid-inducible gene-i-like receptor (rlr) signaling. interestingly, however, ectopic expression of wild-type h-ras results in a biphasic mode of rlr signaling regulation: w ... | 2017 | 28848563 |
| oncolytic alphaviruses in cancer immunotherapy. | oncolytic viruses show specific targeting and killing of tumor cells and therefore provide attractive assets for cancer immunotherapy. in parallel to oncolytic viral vectors based on adenoviruses and herpes simplex viruses, oncolytic rna viruses and particularly alphaviruses have been evaluated as delivery vehicles. immunization studies in experimental rodent models for various cancers including glioblastoma, hematologic, hepatocellular, colon, cervix, and lung cancer as well as melanoma have be ... | 2017 | 28417936 |
| dorsal and ventral parts of thalamic nucleus submedius project to different areas of rat orbitofrontal cortex: a single neuron-tracing study using virus vectors. | the rodent orbitofrontal cortex is involved in a variety of cognitive and behavioral functions that require thalamic input to be successfully expressed. although the thalamic nucleus submedius (sm) is a major source of afferents to the orbitofrontal cortex, thalamocortical projection from the sm has not been fully elucidated. in the present study, we first divided the rat sm into dorsal and ventral parts according to the distribution of vesicular glutamate transporter 2-immunoreactive varicositi ... | 2017 | 28833113 |
| identification of interactions between sindbis virus capsid protein and cytoplasmic vrna as novel virulence determinants. | alphaviruses are arthropod-borne viruses that represent a significant threat to public health at a global level. while the formation of alphaviral nucleocapsid cores, consisting of cargo nucleic acid and the viral capsid protein, is an essential molecular process of infection, the precise interactions between the two partners are ill-defined. a clip-seq approach was used to screen for candidate sites of interaction between the viral capsid protein and genomic rna of sindbis virus (sinv), a model ... | 2017 | 28662211 |
| wolbachia elevates host methyltransferase expression to block an rna virus early during infection. | wolbachia pipientis is an intracellular endosymbiont known to confer host resistance against rna viruses in insects. however, the causal mechanism underlying this antiviral defense remains poorly understood. to this end, we have established a robust arthropod model system to study the tripartite interaction involving sindbis virus and wolbachia strain wmel within its native host, drosophila melanogaster. by leveraging the power of drosophila genetics and a parallel, highly tractable d. melanogas ... | 2017 | 28617844 |
| development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for imaging of replication and immune-mediated changes in intracellular viral rna. | viral rna studies often rely on in situ hybridization and reverse transcriptase-pcr to provide snapshots of rna dynamics in infected cells. to facilitate analysis of cellular rnas, aptamers spinach and spinach2 that bind and activate the conditional fluorophore 3, 5-difluoro-4-hydroxybenzylidene imidazolinon have been developed. to determine the feasibility of applying this technology to viral rna, we have used cdna clones of the te strain of sindbis virus (sinv) to construct multiple viruses co ... | 2017 | 28555544 |
| glutamine antagonist-mediated immune suppression decreases pathology but delays virus clearance in mice during nonfatal alphavirus encephalomyelitis. | infection of weanling c57bl/6 mice with the te strain of sindbis virus (sinv) causes nonfatal encephalomyelitis associated with hippocampal-based memory impairment that is partially prevented by treatment with 6-diazo-5-oxo-l-norleucine (don), a glutamine antagonist (potter et al., j neurovirol 21:159, 2015). to determine the mechanism(s) of protection, lymph node and central nervous system (cns) tissues from sinv-infected mice treated daily for 1 week with low (0.3mg/kg) or high (0.6mg/kg) dose ... | 2017 | 28531865 |
| operational mosquito and vector-borne diseases surveillance at incirlik air base, turkey. | arboviruses on incirlik air base, turkey, pose a threat to military personnel and civilians, but might also be relevant for understanding the threats in neighboring conflict zones such as syria. we reviewed 6 years of mosquito and arbovirus surveillance at incirlik air base. over 6,000 mosquitoes were identified as aedes caspius, anopheles claviger, culex mimeticus, cx. perexiguus, cx. pipiens, cx. sinaiticus, and culiseta longiareolata. almost all of the mosquitoes (more than 90%) were cx. pere ... | 2017 | 28511277 |
| inactivation of viruses by pasteurization at 60 °c for 10 h with and without 40% glucose as stabilizer during a new manufacturing process of α2-macroglobulin from cohn fraction iv. | pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. influence of pasteurization at 60 °c for 10 h on α2-macroglobulin activity and virus inactivation were studied. with 40% sugar as stabilizers more than 70% α2-macroglobulin activity was reserved after pasteurization compared with 20% in control. glucose presented a better activity protection effect than sucrose and maltose. by pasteurization without stabilizer the virus titers of pseudorabies virus, sind ... | 2017 | 28215695 |
| translation of sindbis subgenomic mrna is independent of eif2, eif2a and eif2d. | translation of sindbis virus subgenomic mrna (sgmrna) can occur after inactivation of eif2 by phosphorylation in mammalian cells. several studies have suggested that eif2 can be replaced by eif2a or eif2d. hap1 human cell lines knocked-out for eif2a, eif2d or both by crispr/cas9 genome engineering were compared with wild-type (wt) cells to test the potential role of eif2a and eif2d in translation. sindbis virus infection was comparable between the four cell lines. moreover, synthesis of viral pr ... | 2017 | 28240315 |
| microbiota activates imd pathway and limits sindbis infection in aedes aegypti. | aedes aegypti is the main vector of important arboviruses such as dengue, zika and chikungunya. during infections mosquitoes can activate the immune pathways toll, imd and jak/stat to limit pathogen replication. | 2017 | 28231846 |
| mitochondrial damage elicits a tcdd-inducible poly(adp-ribose) polymerase-mediated antiviral response. | the innate immune system senses rna viruses by pattern recognition receptors (prrs) and protects the host from virus infection. prrs mediate the production of immune modulatory factors and direct the elimination of rna viruses. here, we show a unique prr that mediates antiviral response. tetrachlorodibenzo-p-dioxin (tcdd)-inducible poly(adp ribose) polymerase (tiparp), a cysteine3 histidine (ccch)-type zinc finger-containing protein, binds to sindbis virus (sinv) rna via its zinc finger domain a ... | 2017 | 28213497 |
| neurotropic alphaviruses can propagate without capsid. | 2017 | 28129638 | |
| ablation of programmed -1 ribosomal frameshifting in venezuelan equine encephalitis virus results in attenuated neuropathogenicity. | the alphaviruses venezuelan equine encephalitis virus (veev), eastern equine encephalitis virus (eeev), and western equine encephalitis virus (weev) are arthropod-borne positive-strand rna viruses that are capable of causing acute and fatal encephalitis in many mammals, including humans. veev was weaponized during the cold war and is recognized as a select agent. currently, there are no fda-approved vaccines or therapeutics for these viruses. the spread of veev and other members of this family d ... | 2017 | 27852852 |
| trim25 is required for the antiviral activity of zinc-finger antiviral protein (zap). | zinc-finger antiviral protein (zap) is a host factor that specifically inhibits the replication of certain viruses by binding to viral mrnas, and repressing the translation and/or promoting the degradation of target mrna. in addition, zap regulates the expression of certain cellular genes. here, we report that tripartite motif-containing protein 25 (trim25), a ubiquitin e3 ligase, is required for the antiviral activity of zap. downregulation of endogenous trim25 abolished zap's antiviral activit ... | 2017 | 28202764 |
| an icosahedral virus as a fluorescent calibration standard: a method for counting protein molecules in cells by fluorescence microscopy. | the ability to replace genes coding for cellular proteins with dna that codes for fluorescent protein-tagged versions opens the way to counting the number of molecules of each protein component of macromolecular assemblies in vivo by measuring fluorescence microscopically. converting fluorescence to absolute numbers of molecules requires a fluorescent standard whose molecular composition is known precisely. in this report, the construction, properties and mode of using a set of fluorescence cali ... | 2017 | 28328099 |
| dynamics of molecular evolution in rna virus populations depend on sudden versus gradual environmental change. | understanding the dynamics of molecular adaptation is a fundamental goal of evolutionary biology. while adaptation to constant environments has been well characterized, the effects of environmental complexity remain seldom studied. one simple but understudied factor is the rate of environmental change. here we used experimental evolution with rna viruses to investigate whether evolutionary dynamics varied based on the rate of environmental turnover. we used whole-genome next-generation sequencin ... | 2017 | 28121018 |
| trim25 enhances the antiviral action of zinc-finger antiviral protein (zap). | the host factor and interferon (ifn)-stimulated gene (isg) product, zinc-finger antiviral protein (zap), inhibits a number of diverse viruses by usurping and intersecting with multiple cellular pathways. to elucidate its antiviral mechanism, we perform a loss-of-function genome-wide rnai screen to identify cellular cofactors required for zap antiviral activity against the prototype alphavirus, sindbis virus (sinv). in order to exclude off-target effects, we carry out stringent confirmatory assay ... | 2017 | 28060952 |
| spatial and temporal analysis of alphavirus replication and assembly in mammalian and mosquito cells. | sindbis virus (sinv [genus alphavirus, family togaviridae]) is an enveloped, mosquito-borne virus. alphaviruses cause cytolytic infections in mammalian cells while establishing noncytopathic, persistent infections in mosquito cells. mosquito vector adaptation of alphaviruses is a major factor in the transmission of epidemic strains of alphaviruses. though extensive studies have been performed on infected mammalian cells, the morphological and structural elements of alphavirus replication and ass ... | 2017 | 28196962 |
| palmitoylation of sindbis virus tf protein regulates its plasma membrane localization and subsequent incorporation into virions. | palmitoylation is a reversible, posttranslational modification that helps target proteins to cellular membranes. the alphavirus small membrane proteins 6k and tf have been reported to be palmitoylated and to positively regulate budding. 6k and tf are isoforms that are identical in their n termini but unique in their c termini due to a -1 ribosomal frameshift during translation. in this study, we used cysteine (cys) mutants to test differential palmitoylation of the sindbis virus 6k and tf protei ... | 2017 | 27852864 |
| individual mediodorsal thalamic neurons project to multiple areas of the rat prefrontal cortex: a single neuron-tracing study using virus vectors. | the prefrontal cortex has an important role in a variety of cognitive and executive processes, and is generally defined by its reciprocal connections with the mediodorsal thalamic nucleus (md). the rat md is mainly subdivided into three segments, the medial (mdm), central (mdc), and lateral (mdl) divisions, on the basis of the cytoarchitecture and chemoarchitecture. the md segments are known to topographically project to multiple prefrontal areas at the population level: the mdm mainly to the pr ... | 2017 | 27275581 |
| discovery of a novel antiviral agent targeting the nonstructural protein 4 (nsp4) of chikungunya virus. | chikungunya fever (chikf) is caused by chikungunya virus (chikv) infection which is a re-emerging mosquito-borne zoonosis. at present, there are no approved therapeutics for chikf. herein, we have investigated candidate compounds which can inhibit chikv infection. screening of chemical compound libraries were performed and one candidate, a benzimidazole-related compound designated compound-a was found to inhibit infection by several chikv strains and a sindbis virus strain at nanomolar concentra ... | 2017 | 28236746 |
| alphavirus-based vaccines. | alphavirus-based vectors have been engineered from semliki forest virus, sindbis virus, and venezuelan equine encephalitis virus and applied for vaccine development. immunization in preclinical animal models has been conducted with naked rna replicons, recombinant viral particles and layered dna-rna vectors. most commonly, the targets for the immunization have been viral surface proteins and tumor antigens, which have elicited strong immune responses and even provided protection against challeng ... | 2017 | 28374252 |
| selective transduction of astrocytic and neuronal cns subpopulations by lentiviral vectors pseudotyped with chikungunya virus envelope. | lentiviral vectors are gene delivery vehicles that integrate into the host genome of dividing and non-dividing mammalian cells facilitating long-term transgene expression. lentiviral vector versatility is greatly increased by incorporating heterologous viral envelope proteins onto the vector particles instead of the native envelope, conferring on these pseudotyped vectors a modified tropism and host range specificity. we investigated the pseudotyping efficiency of hiv-1 based lentiviral vectors ... | 2017 | 28152379 |
| obatoclax inhibits alphavirus membrane fusion by neutralizing the acidic environment of endocytic compartments. | as new pathogenic viruses continue to emerge, it is paramount to have intervention strategies that target a common denominator in these pathogens. the fusion of viral and cellular membranes during viral entry is one such process that is used by many pathogenic viruses, including chikungunya virus, west nile virus, and influenza virus. obatoclax, a small-molecule antagonist of the bcl-2 family of proteins, was previously determined to have activity against influenza a virus and also sindbis virus ... | 2017 | 27993855 |
| mapping of human b-cell epitopes of sindbis virus. | mosquito-transmitted sindbis virus (sinv) causes fever, skin lesions and musculoskeletal symptoms if transmitted to man. sinv is the prototype virus of genus alphavirus, which includes other arthritogenic viruses such as chikungunya virus (chikv) and ross river virus (rrv) that cause large epidemics with a considerable public health burden. until now the human b-cell epitopes have been studied for chikv and rrv, but not for sinv. to identify the b-cell epitopes in sinv-infection, we synthetised ... | 2016 | 27339177 |
| the presence and seroprevalence of arthropod-borne viruses in nasiriyah governorate, southern iraq: a cross-sectional study. | the knowledge on the presence and seroprevalence of arboviruses in iraq is fragmental. to assess the exposure of the population to arbovirus infections in southern iraq, we conducted a serological screening of the most common arbovirus groups using immunofluorescence, hemagglutination inhibition and neutralization tests. serum samples of 399 adult volunteers were collected in nasiriyah, iraq. antibodies were detected against west nile virus (wnv) (11.6%), sandfly-borne sicilian virus serocomplex ... | 2016 | 26880770 |
| alphavirus protease inhibitors from natural sources: a homology modeling and molecular docking investigation. | alphaviruses such as chikungunya virus (chikv), o'nyong-nyong virus (onnv), ross river virus (rrv), eastern equine encephalitis virus (eeev), venezuelan equine encephalitis virus (veev), and western equine encephalitis virus (weev), are mosquito-transmitted viruses that can cause fevers, rash, and rheumatic diseases (chikv, onnv, rrv) or potentially fatal encephalitis (eeev, veev, weev) in humans. these diseases are considered neglected tropical diseases for which there are no current antiviral ... | 2016 | 27387412 |
| an mrna-specific trnai carrier eif2a plays a pivotal role in cell proliferation under stress conditions: stress-resistant translation of c-src mrna is mediated by eif2a. | c-src, a non-receptor protein tyrosine kinase, activates nf-κb and stat3, which in turn triggers the transcription of anti-apoptosis- and cell cycle-related genes. c-src protein regulates cell proliferation, cell motility and programmed cell death. and the elevated level of activated c-src protein is related with solid tumor generation. translation of c-src mrna is directed by an ires element which mediates persistent translation under stress conditions when translation of most mrnas is inhibite ... | 2016 | 27899592 |
| identification of a nucleotide in 5' untranslated region contributing to virus replication and virulence of coxsackievirus a16. | coxsackievirus a16 (ca16) and enterovirus 71 (ev71) are two main causative pathogens of hand, foot and mouth disease (hfmd). unlike ev71, virulence determinants of ca16, particularly within 5' untranslated region (5'utr), have not been investigated until now. here, a series of nucleotides present in 5'utr of lethal but not in non-lethal ca16 strains were screened by aligning nucleotide sequences of lethal circulating changchun ca16 and the prototype g10 as well as non-lethal shzh05 strains. a re ... | 2016 | 26861413 |
| activation of rnase l is dependent on oas3 expression during infection with diverse human viruses. | the 2',5'-oligoadenylate (2-5a) synthetase (oas)-rnase l system is an ifn-induced antiviral pathway. rnase l activity depends on 2-5a, synthesized by oas. although all three enzymatically active oas proteins in humans--oas1, oas2, and oas3--synthesize 2-5a upon binding dsrna, it is unclear which are responsible for rnase l activation during viral infection. we used clustered regularly interspaced short palindromic repeats (crispr)-crispr-associated protein-9 nuclease (cas9) technology to enginee ... | 2016 | 26858407 |
| yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna. | mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ... | 2016 | 27849599 |
| peroxisomal protein pex13 functions in selective autophagy. | pex13 is an integral membrane protein on the peroxisome that regulates peroxisomal matrix protein import during peroxisome biogenesis. mutations in pex13 and other peroxin proteins are associated with zellweger syndrome spectrum (zss) disorders, a subtype of peroxisome biogenesis disorder characterized by prominent neurological, hepatic, and renal abnormalities leading to neonatal death. the lack of functional peroxisomes in zss patients is widely accepted as the underlying cause of disease; how ... | 2016 | 27827795 |
| deep sequencing analysis reveals the mycoviral diversity of the virome of an avirulent isolate of rhizoctonia solani ag-2-2 iv. | rhizoctonia solani represents an important plant pathogenic basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsrna elements in natural populations of the fungus. to date, eight different mycoviruses have been characterized in rhizoctonia and some of them have been reported to modulate its virulence. dsrna extracts of the avirulent r. solani isolate dc17 (ag2-2-iv) displayed a diverse pattern, indicating multiple infections with mycov ... | 2016 | 27814394 |
| genome investigations of vector competence in aedes aegypti to inform novel arbovirus disease control approaches. | dengue (denv), yellow fever, chikungunya, and zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, aedes aegypti. the "vectorial capacity" of a. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmi ... | 2016 | 27809220 |
| climate change influences on the global potential distribution of the mosquito culex quinquefasciatus, vector of west nile virus and lymphatic filariasis. | rapid emergence of most vector-borne diseases (vbds) may be associated with range expansion of vector populations. culex quinquefasciatus say 1823 is a potential vector of west nile virus, saint louis encephalitis virus, and lymphatic filariasis. we estimated the potential distribution of cx. quinquefasciatus under both current and future climate conditions. the present potential distribution of cx. quinquefasciatus showed high suitability across low-latitude parts of the world, reflecting the c ... | 2016 | 27695107 |
| a 3'-end structure in rna2 of a crinivirus is essential for viral rna synthesis and contributes to replication-associated translation activity. | the terminal ends in the genome of rna viruses contain features that regulate viral replication and/or translation. we have identified a y-shaped structure (yss) in the 3' terminal regions of the bipartite genome of lettuce chlorosis virus (lcv), a member in the genus crinivirus (family closteroviridae). the yss is the first in this family of viruses to be determined using selective 2'-hydroxyl acylation analyzed by primer extension (shape). using luciferase constructs/replicons, in vivo and in ... | 2016 | 27694962 |
| differential expression pattern of vago in bumblebee (bombus terrestris), induced by virulent and avirulent virus infections. | viruses are one of the main drivers of the decline of domesticated and wild bees but the mechanisms of antiviral immunity in pollinators are poorly understood. recent work has suggested that next to the small interfering rna (sirna) pathway other immune-related pathways play a role in the defense of the bee hosts against viral infection. in addition, vago plays a role in the cross-talk between the innate immune pathways in culex mosquito cells. here we describe the vago orthologue in bumblebees ... | 2016 | 27680717 |
| quantification of virus particles using nanopore-based resistive-pulse sensing techniques. | viruses have drawn much attention in recent years due to increased recognition of their important roles in virology, immunology, clinical diagnosis, and therapy. because the biological and physical properties of viruses significantly impact their applications, quantitative detection of individual virus particles has become a critical issue. however, due to various inherent limitations of conventional enumeration techniques such as infectious titer assays, immunological assays, and electron micro ... | 2016 | 27713738 |
| morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus. | candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ... | 2016 | 27630042 |
| molecular evolution of type 2 porcine reproductive and respiratory syndrome viruses circulating in vietnam from 2007 to 2015. | porcine respiratory and reproductive syndrome (prrs) virus is one of the most economically significant pathogens in the vietnamese swine industry. orf5, which participates in many functional processes, including virion assembly, entry of the virus into the host cell, and viral adaptation to the host immune response, has been widely used in molecular evolution and phylogeny studies. knowing of molecular evolution of prrsv fields strains might contribute to prrs control in vietnam. | 2016 | 27855667 |
| the conserved coronavirus macrodomain promotes virulence and suppresses the innate immune response during severe acute respiratory syndrome coronavirus infection. | adp-ribosylation is a common posttranslational modification that may have antiviral properties and impact innate immunity. to regulate this activity, macrodomain proteins enzymatically remove covalently attached adp-ribose from protein targets. all members of the coronavirinae, a subfamily of positive-sense rna viruses, contain a highly conserved macrodomain within nonstructural protein 3 (nsp3). however, its function or targets during infection remain unknown. we identified several macrodomain ... | 2016 | 27965448 |
| first report on human-biting culex pipiens in sweden. | culex mosquitoes are vectors of several bird-hosted arboviruses that cause outbreaks in europe, such as sindbis virus and west nile virus. recently, the human-biting form of culex pipiens, cx. pipiens biotype molestus, was found causing big nuisance in a housing cooperative in gothenburg in southern sweden, confirmed by molecular identification. this is the first report of human-biting culex in scandinavia, signalling increased risk of arbovirus infection in northern europe. | 2016 | 27927233 |
| high insecticides resistance in culex pipiens (diptera: culicidae) from tehran, capital of iran. | during recent years transmission of dirofilaria immitis (dog heart worm) by culex pipiens and west nile virus have been reported from iran. the present study was preformed for evaluating the susceptibility status of cx. pipiens collected from capital city of tehran, iran. | 2016 | 28032100 |
| acyclic nucleoside phosphonates containing the amide bond. | to study the influence of a linker rigidity and donor-acceptor properties, the p-ch2-o-chr- fragment in acyclic nucleoside phosphonates (e.g., acyclovir, tenofovir) was replaced by the p-ch2-hn-c(o)- residue. the respective phosphonates were synthesized in good yields by coupling the straight chain of ω-aminophosphonates and nucleobase-derived acetic acids with edc. based on the (1)h and (13)c nmr data, the unrestricted rotation within the methylene and 1,2-ethylidene linkers in phosphonates fro ... | 2016 | 27881885 |
| crystal structure of glycoprotein c from a hantavirus in the post-fusion conformation. | hantaviruses are important emerging human pathogens and are the causative agents of serious diseases in humans with high mortality rates. like other members in the bunyaviridae family their m segment encodes two glycoproteins, gn and gc, which are responsible for the early events of infection. hantaviruses deliver their tripartite genome into the cytoplasm by fusion of the viral and endosomal membranes in response to the reduced ph of the endosome. unlike phleboviruses (e.g. rift valley fever vi ... | 2016 | 27783673 |
| mutagenic effects of ribavirin on hepatitis e virus-viral extinction versus selection of fitness-enhancing mutations. | hepatitis e virus (hev), an important agent of viral hepatitis worldwide, can cause severe courses of infection in pregnant women and immunosuppressed patients. to date, hev infections can only be treated with ribavirin (rbv). major drawbacks of this therapy are that rbv is not approved for administration to pregnant women and that the virus can acquire mutations, which render the intra-host population less sensitive or even resistant to rbv. one of the proposed modes of action of rbv is a direc ... | 2016 | 27754363 |
| excess cerebral tnf causing glutamate excitotoxicity rationalizes treatment of neurodegenerative diseases and neurogenic pain by anti-tnf agents. | the basic mechanism of the major neurodegenerative diseases, including neurogenic pain, needs to be agreed upon before rational treatments can be determined, but this knowledge is still in a state of flux. most have agreed for decades that these disease states, both infectious and non-infectious, share arguments incriminating excitotoxicity induced by excessive extracellular cerebral glutamate. excess cerebral levels of tumor necrosis factor (tnf) are also documented in the same group of disease ... | 2016 | 27596607 |
| isg12a restricts hepatitis c virus infection through the ubiquitination-dependent degradation pathway. | interferons (ifns) restrict various kinds of viral infection via induction of hundreds of ifn-stimulated genes (isgs), while the functions of the majority of isgs are broadly unclear. here, we show that a high-ifn-inducible gene, isg12a (also known as ifi27), exhibits a nonapoptotic antiviral effect on hepatitis c virus (hcv) infection. viral ns5a protein is targeted specifically by isg12a, which mediates ns5a degradation via a ubiquitination-dependent proteasomal pathway. k374r mutation in ns5a ... | 2016 | 27194766 |
| mechanisms of viral mutation. | the remarkable capacity of some viruses to adapt to new hosts and environments is highly dependent on their ability to generate de novo diversity in a short period of time. rates of spontaneous mutation vary amply among viruses. rna viruses mutate faster than dna viruses, single-stranded viruses mutate faster than double-strand virus, and genome size appears to correlate negatively with mutation rate. viral mutation rates are modulated at different levels, including polymerase fidelity, sequence ... | 2016 | 27392606 |
| trim14 inhibits hepatitis c virus infection by spry domain-dependent targeted degradation of the viral ns5a protein. | tripartite motif 14 (trim14) was reported to function as a mitochondrial signaling adaptor in mediating innate immune responses. however, the involvement of trim14 in host defense against viral infection and molecular mechanisms remain unclear. here, we demonstrated that enforced expression of trim14 could potently inhibit the infection and replication of hcv in hepatocytes, whereas trim14 knockout cells became more susceptible to hcv infection. interestingly, further experiments revealed that s ... | 2016 | 27578425 |
| tnfα impairs rhabdoviral clearance by inhibiting the host autophagic antiviral response. | tnfα is a pleiotropic pro-inflammatory cytokine with a key role in the activation of the immune system to fight viral infections. despite its antiviral role, a few viruses might utilize the host produced tnfα to their benefit. some recent reports have shown that anti-tnfα therapies could be utilized to treat certain viral infections. however, the underlying mechanisms by which tnfα can favor virus replication have not been identified. here, a rhabdoviral infection model in zebrafish allowed us t ... | 2016 | 27351838 |
| the strica homolog aacasps16 is involved in apoptosis in the yellow fever vector, aedes albopictus. | caspases are a family of cysteine proteases playing essential roles during apoptosis. seven caspases identified in drosophila were dronc, dredd, strica, dcp-1, decay, drice and damm. among them, strica is an insect-specific caspase containing a long serine- and threonine- rich prodomain, of which function is not yet well studied. here we identified a homolog of strica from aedes albopictus, named as aacasps16. aacasps16 encoded a protein containing a putative serine- and threonine-rich prodomain ... | 2016 | 27351972 |
| interferon gamma: influence on neural stem cell function in neurodegenerative and neuroinflammatory disease. | interferon-gamma (ifnγ), a pleiotropic cytokine, is expressed in diverse neurodegenerative and neuroinflammatory conditions. its protective mechanisms are well documented during viral infections in the brain, where ifnγ mediates non-cytolytic viral control in infected neurons. however, ifnγ also plays both protective and pathological roles in other central nervous system (cns) diseases. of the many neural cells that respond to ifnγ, neural stem/progenitor cells (nspcs), the only pluripotent cell ... | 2016 | 27774000 |
| amyloid-β effects on synapses and memory require ampa receptor subunit glua3. | amyloid-β (aβ) is a prime suspect for causing cognitive deficits during the early phases of alzheimer's disease (ad). experiments in ad mouse models have shown that soluble oligomeric clusters of aβ degrade synapses and impair memory formation. we show that all aβ-driven effects measured in these mice depend on ampa receptor (ampar) subunit glua3. hippocampal neurons that lack glua3 were resistant against aβ-mediated synaptic depression and spine loss. in addition, aβ oligomers blocked long-term ... | 2016 | 27708157 |
| recent advances in lentiviral vaccines for hiv-1 infection. | the development of an effective hiv vaccine to prevent and/or cure hiv remains a global health priority. given their central role in the initiation of adaptive immune responses, dendritic cell (dc)-based vaccines are being increasingly explored as immunotherapeutic strategies to enhance hiv-specific t cells in infected individuals and, thus, promote immune responses that may help facilitate a functional cure. hiv-1-based lentiviral (lv) vectors have inherent advantages as dc vaccine vectors due ... | 2016 | 27446074 |
| mosquito saliva increases endothelial permeability in the skin, immune cell migration, and dengue pathogenesis during antibody-dependent enhancement. | dengue remains the most prevalent arthropod-borne viral disease in humans. while probing for blood vessels, aedes aegypti and ae. albopictus mosquitoes transmit the four serotypes of dengue virus (denv1-4) by injecting virus-containing saliva into the skin. even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens, the full impact of mosquito saliva on dengue pathogenesis is still not well understood. inoculating mice lacking the interferon-α ... | 2016 | 27310141 |
| global profiling of the cellular alternative rna splicing landscape during virus-host interactions. | alternative splicing (as) is a central mechanism of genetic regulation which modifies the sequence of rna transcripts in higher eukaryotes. as has been shown to increase both the variability and diversity of the cellular proteome by changing the composition of resulting proteins through differential choice of exons to be included in mature mrnas. in the present study, alterations to the global rna splicing landscape of cellular genes upon viral infection were investigated using mammalian reoviru ... | 2016 | 27598998 |
| consecutive inhibition of isg15 expression and isgylation by cytomegalovirus regulators. | interferon-stimulated gene 15 (isg15) encodes an ubiquitin-like protein that covalently conjugates protein. protein modification by isg15 (isgylation) is known to inhibit the replication of many viruses. however, studies on the viral targets and viral strategies to regulate isgylation-mediated antiviral responses are limited. in this study, we show that human cytomegalovirus (hcmv) replication is inhibited by isgylation, but the virus has evolved multiple countermeasures. hcmv-induced isg15 expr ... | 2016 | 27564865 |
| receptor-targeted nipah virus glycoproteins improve cell-type selective gene delivery and reveal a preference for membrane-proximal cell attachment. | receptor-targeted lentiviral vectors (lvs) can be an effective tool for selective transfer of genes into distinct cell types of choice. moreover, they can be used to determine the molecular properties that cell surface proteins must fulfill to act as receptors for viral glycoproteins. here we show that lvs pseudotyped with receptor-targeted nipah virus (niv) glycoproteins effectively enter into cells when they use cell surface proteins as receptors that bring them closely enough to the cell memb ... | 2016 | 27281338 |
| type-1 angiotensin receptor signaling in central nervous system myeloid cells is pathogenic during fatal alphavirus encephalitis in mice. | alphaviruses can cause fatal encephalitis in humans. natural infections occur via the bite of infected mosquitos, but aerosol transmissibility makes some of these viruses potential bioterrorism agents. central nervous system (cns) host responses contribute to alphavirus pathogenesis in experimental models and are logical therapeutic targets. we investigated whether reactive oxygen species (ros) generated by nicotinamide adenine dinucleotide phosphate (nadph) oxidase (nox) activity within the cns ... | 2016 | 27562117 |
| working with zika and usutu viruses in vitro. | usutu (usuv) and zika (zikv) viruses are emerging arboviruses of significant medical and veterinary importance. these viruses have not been studied as well as other medically important arboviruses such as west nile (wnv), dengue (denv), or chikungunya (chikv) viruses. as such, information regarding the behavior of zikv and usuv viruses in the laboratory is dated. usutu virus re-emerged in austria in 2001 and has since spread throughout the european and asian continents causing significant mortal ... | 2016 | 27541001 |
| entry sites of venezuelan and western equine encephalitis viruses in the mouse central nervous system following peripheral infection. | venezuelan and western equine encephalitis viruses (veev and weev; alphavirus; togaviridae) are mosquito-borne pathogens causing central nervous system (cns) disease in humans and equids. adult cd-1 mice also develop cns disease after infection with veev and weev. adult cd-1 mice infected by the intranasal (i.n.) route, showed that veev and weev enter the brain through olfactory sensory neurons (osns). in this study, we injected the mouse footpad with recombinant weev (mcmillan) or veev (subtype ... | 2016 | 27053560 |
| suppression of usp18 potentiates the anti-hbv activity of interferon alpha in hepg2.2.15 cells via jak/stat signaling. | ubiquitin-specific protease 18 (usp18, also known as ubp43) has both interferon stimulated gene 15 (isg15) dependent and isg15-independent functions. by silencing the expression of usp18 in hepg2.2.15 cells, we studied the effect of usp18 on the anti-hbv activity of ifn-f and demonstrated that knockdown of usp18 significantly inhibited the hbv expression and increased the expression of isgs. levels of hepatitis b virus surface antigen (hbsag), hepatitis b virus e antigen (hbeag), hbv dna and int ... | 2016 | 27227879 |
| infection pattern and transmission potential of chikungunya virus in two new world laboratory-adapted aedes aegypti strains. | chikungunya virus (chikv) is an emerging mosquito-borne virus belonging to the togaviridae, which is transmitted to humans by aedes aegypti and ae. albopictus. we describe the infection pattern of chikv in two new world ae. aegypti strains, hwe and orl. both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious addition ... | 2016 | 27102548 |
| pilot longitudinal mosquito surveillance study in the danube delta biosphere reserve and the first reports of anopheles algeriensis theobald, 1903 and aedes hungaricus mihályi, 1955 for romania. | mosquito-borne viruses (moboviruses) are of growing importance in many countries of europe. in romania and especially in the danube delta biosphere reserve (ddbr), mosquito and mobovirus surveillance are not performed on a regular basis. however, this type of study is crucially needed to evaluate the risk of pathogen transmission, to understand the ecology of emerging moboviruses, or to plan vector control programmes. | 2016 | 27066827 |
| ecological characterization and molecular differentiation of culex pipiens complex taxa and culex torrentium in eastern austria. | culex pipiens complex taxa differ in behaviour, ecophysiology and epidemiologic importance. despite their epidemiologic significance, information on genetic diversity, occurrence and seasonal and spatial distribution patterns of the cx. pipiens complex is still insufficient. assessment of seasonal and spatial distribution patterns of culex pipiens forms and their congener cx. torrentium is crucial for the understanding of their vector-pathogen dynamics. | 2016 | 27067139 |
| a new defective helper rna to produce recombinant sindbis virus that infects neurons but does not propagate. | recombinant sindbis viruses are important tools in neuroscience because they combine rapid and high transgene expression with a capacity to carry large transgenes. currently, two packaging systems based on the defective helper (dh) rnas dh(26s)5'sin and dh-bb(trna;te12) are available for generating recombinant sindbis virus that is neurotropic (able to infect neurons and potentially other cells). both systems produce a fraction of viral particles that can propagate beyond the primary infected ne ... | 2016 | 27252627 |
| hepatitis c virus infection induces autophagy as a prosurvival mechanism to alleviate hepatic er-stress response. | hepatitis c virus (hcv) infection frequently leads to chronic liver disease, liver cirrhosis and hepatocellular carcinoma (hcc). the molecular mechanisms by which hcv infection leads to chronic liver disease and hcc are not well understood. the infection cycle of hcv is initiated by the attachment and entry of virus particles into a hepatocyte. replication of the hcv genome inside hepatocytes leads to accumulation of large amounts of viral proteins and rna replication intermediates in the endopl ... | 2016 | 27223299 |
| functional translational readthrough: a systems biology perspective. | translational readthrough (tr) has come into renewed focus because systems biology approaches have identified the first human genes undergoing functional translational readthrough (ftr). ftr creates functional extensions to proteins by continuing translation of the mrna downstream of the stop codon. here we review recent developments in tr research with a focus on the identification of ftr in humans and the systems biology methods that have spurred these discoveries. | 2016 | 27490485 |
| cytoplasmic drosha activity generated by alternative splicing. | rnase iii enzyme drosha interacts with dgcr8 to form the microprocessor, initiating canonical microrna (mirna) maturation in the nucleus. here, we re-evaluated where drosha functions in cells using drosha and/or dgcr8 knock out (ko) cells and cleavage reporters. interestingly, a truncated drosha mutant located exclusively in the cytoplasm cleaved pri-mirna effectively in a dgcr8-dependent manner. in addition, we demonstrated that in vitro generated pri-mirnas when transfected into cells could be ... | 2016 | 27471035 |
| a de novo transcriptome analysis shows that modulation of the jak-stat signaling pathway by salmonid alphavirus subtype 3 favors virus replication in macrophage/dendritic-like to-cells. | the janus kinase (jak) and signaling transducer activator of transcription (stat) pathway mediates the signaling of genes required for cellular development and homeostasis. to elucidate the effect of type i ifn on the jak/stat pathway in salmonid alphavirus subtype 3 (sav3) infected macrophage/dendritic like to-cells derived from atlantic salmon (salmo salar l) headkidney leukocytes, we used a differential transcriptome analysis by rna-seq and the kyoto encyclopedia of genes and genomes (keggs) ... | 2016 | 27215196 |
| a universal next-generation sequencing protocol to generate noninfectious barcoded cdna libraries from high-containment rna viruses. | several biosafety level 3 and/or 4 (bsl-3/4) pathogens are high-consequence, single-stranded rna viruses, and their genomes, when introduced into permissive cells, are infectious. moreover, many of these viruses are select agents (sas), and their genomes are also considered sas. for this reason, cdnas and/or their derivatives must be tested to ensure the absence of infectious virus and/or viral rna before transfer out of the bsl-3/4 and/or sa laboratory. this tremendously limits the capacity to ... | 2016 | 27822536 |
| deah-rha helicase•znf cofactor systems in kinetoplastid rna editing and evolutionarily distant rna processes. | multi-zinc finger proteins are an emerging class of cofactors in deah-rha rna helicases across highly divergent eukaryotic lineages. deah-rha helicase•zinc finger cofactor partnerships predate the split of kinetoplastid protozoa, which include several human pathogens, from other eukaryotic lineages 100-400 ma. despite a long evolutionary history, the prototypical deah-rha domains remain highly conserved. this short review focuses on a recently identified deah-rha helicase•zinc finger cofactor sy ... | 2016 | 27540585 |
| a tale of two rnas during viral infection: how viruses antagonize mrnas and small non-coding rnas in the host cell. | viral infection initiates an array of changes in host gene expression. many viruses dampen host protein expression and attempt to evade the host anti-viral defense machinery. host gene expression is suppressed at several stages of host messenger rna (mrna) formation including selective degradation of translationally competent messenger rnas. besides mrnas, host cells also express a variety of noncoding rnas, including small rnas, that may also be subject to inhibition upon viral infection. in th ... | 2016 | 27271653 |
| a glance at subgenomic flavivirus rnas and micrornas in flavivirus infections. | the family flaviviridae comprises a wide variety of viruses that are distributed worldwide, some of which are associated with high rates of morbidity and mortality. there are neither vaccines nor antivirals for most flavivirus infections, reinforcing the importance of research on different aspects of the viral life cycle. during infection, cytoplasmic accumulation of rna fragments mainly originating from the 3' utrs, which have been designated subgenomic flavivirus rnas (sfrnas), has been detect ... | 2016 | 27233361 |
| use of aptamers as diagnostics tools and antiviral agents for human viruses. | appropriate diagnosis is the key factor for treatment of viral diseases. time is the most important factor in rapidly developing and epidemiologically dangerous diseases, such as influenza, ebola and sars. chronic viral diseases such as hiv-1 or hcv are asymptomatic or oligosymptomatic and the therapeutic success mainly depends on early detection of the infective agent. over the last years, aptamer technology has been used in a wide range of diagnostic and therapeutic applications and, concretel ... | 2016 | 27999271 |
| the role of phlebovirus glycoproteins in viral entry, assembly and release. | bunyaviruses are enveloped viruses with a tripartite rna genome that can pose a serious threat to animal and human health. members of the phlebovirus genus of the family bunyaviridae are transmitted by mosquitos and ticks to humans and include highly pathogenic agents like rift valley fever virus (rvfv) and severe fever with thrombocytopenia syndrome virus (sftsv) as well as viruses that do not cause disease in humans, like uukuniemi virus (uukv). phleboviruses and other bunyaviruses use their e ... | 2016 | 27455305 |
| amphotericin b inhibits enterovirus 71 replication by impeding viral entry. | enterovirus 71 (ev71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. there is thus an urgent need to find new treatments to control ev71 infection. in this study, we report potent inhibition of ev71 by a polyene antibiotic amphotericin b. amphotericin b profoundly diminished the expression of ev71 rna and viral proteins in the rd cells and the hek293 cells. as a result, ev71 production was inhibited by amphotericin b with an ... | 2016 | 27608771 |
| m2bp inhibits hiv-1 virion production in a vimentin filaments-dependent manner. | m2bp (also called 90k) is an interferon-stimulated gene product that is upregulated in hiv-1 infection. a recent study revealed that m2bp reduces the infectivity of hiv-1 by inhibiting the processing of the viral envelope protein. here we report that in addition to reducing viral infectivity, m2bp inhibits hiv-1 virion production. we provide evidence showing that m2bp inhibits hiv-1 gag trafficking to the plasma membrane in a vimentin-dependent manner. when vimentin filaments were collapsed by t ... | 2016 | 27604950 |
| exaptive origins of regulated mrna decay in eukaryotes. | eukaryotic gene expression is extensively controlled at the level of mrna stability and the mechanisms underlying this regulation are markedly different from their archaeal and bacterial counterparts. we propose that two such mechanisms, nonsense-mediated decay (nmd) and motif-specific transcript destabilization by ccch-type zinc finger rna-binding proteins, originated as a part of cellular defense against rna pathogens. these branches of the mrna turnover pathway might have been used by primeva ... | 2016 | 27438915 |
| a simple and efficient in vivo non-viral rna transfection method for labeling the whole axonal tree of individual adult long-range projection neurons. | we report a highly efficient, simple, and non-infective method for labeling individual long-range projection neurons (lrpns) in a specific location with enough sparseness and intensity to allow complete and unambiguous reconstructions of their entire axonal tree. the method is based on the "in vivo" transfection of a large rna construct that drives the massive expression of green fluorescent protein. the method combines two components: injection of a small volume of a hyperosmolar nacl solution ... | 2016 | 27047347 |
| autophagy in plasma cell ontogeny and malignancy. | autophagy is a highly conserved pathway that recycles cytosolic material and organelles via lysosomal degradation. once simplistically viewed as a non-selective survival strategy in dire straits, autophagy has emerged as a tightly regulated process ensuring organelle function, proteome plasticity, cell differentiation and tissue homeostasis, with key roles in physiology and disease. selective target recognition, mediated by specific adapter proteins, enables autophagy to orchestrate highly speci ... | 2016 | 26984755 |
| endogenously-expressed nh2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands. | the circumsporozoite protein is the most abundant polypeptide expressed by sporozoites, the malaria parasite stage capable of infecting humans. sporozoite invasion of mosquito salivary glands prior to transmission is likely mediated by a receptor/ligand-like interaction of the parasites with the target tissues, and the amino (nh2)-terminal portion of csp is involved in this interaction but not the tsr region on the carboxyl (c)-terminus. peptides based on the nh2-terminal domain could compete wi ... | 2016 | 26964736 |
| filopodia and viruses: an analysis of membrane processes in entry mechanisms. | filopodia are thin, actin rich bundles protruding from cell plasma membranes, serving physiological purposes, such as probing the environment and facilitating cell-to-cell adhesion. recent studies have highlighted that actively polymerized filopodial-protrusions are exploited during virus entry, trafficking, spread, and the development of clinical pathology of viral diseases. these observations have caused a surge in investigation of the key determinants of filopodial induction and their influen ... | 2016 | 27014223 |