Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| pathogenesis of infectious bursal disease in embryonally bursectomised chickens. | embryonally bursectomised and nonbursectomised chickens were infected with infectious bursal disease virus (ibdv) at 36 days of age. neither clinical signs nor gross lesions were observed in the infected, bursectomised (ib) chickens. no significant changes were observed in carcass, thymus or spleen weights of ib and noninfected bursectomised chickens. a mild lymphocytic necrosis and depletion were found in the spleen, thymus and caecal tonsil of the ib chickens. neither precipitating nor serum n ... | 1990 | 18679966 |
| effect of infectious bursal disease virus on infections produced by escherichia coli of high and low virulence in chickens. | the effect of infectious bursal disease virus (ibdv) on the infections caused by escherichia coli strains of high (expt 1) and low (expt 2) virulence was examined in specific-pathogen-free chickens. the chickens were inoculated orally with ibdv at 1 day of age and via the air sac with e. coli at 1 week of age. in the groups given 1 x 10(5) cfu of e.coli of high virulence (expt 1), mortality of ibdv-inoculated group (90%) was significantly higher than that in the non-ibdv-inoculated group (40%). ... | 1990 | 18679984 |
| pathogenicity of avian nephritis virus in chicks previously infected with infectious bursal disease virus. | pathogenicity of avian nephritis virus (anv) was studied in 7-day-old chicks previously infected with infectious bursal disease virus (ibdv) at 1 day of age. three ibdv+anv infected chicks were found dead with no apparent clinical signs within 10 days of inoculation with anv and they had marked urate deposits throughout the body. all of the surviving ibdv+anv infected chicks had reduced body weight gain, unlike the anv-only infected, ibdv-only infected and non-infected control chicks. in a chron ... | 1991 | 18680003 |
| a recombinant subunit vaccine that protects progeny chickens from infectious bursal disease. | the major protective immunogen of infectious bursal disease virus (ibdv), vp2, was produced in a highly immunogenic form by expression in the yeast saccharomyces cerevisiae. the recombinant protein, formulated as an oil-emulsion vaccine, induced both virus neutralising and elisa antibodies in specific pathogen free hens. these antibodies were passed, via the egg yolk, to progeny chickens and protected them against a challenge infection with virulent ibdv. the protective efficacy of maternal anti ... | 1991 | 18680041 |
| acute infectious bursal disease in poultry: isolation and characterisation of a highly virulent strain. | a highly virulent strain of infectious bursal disease virus (ibdv) was isolated from the field and propagated in spf chickens, causing up to 100% mortality. although it still belongs to the standard serotype 1 ibd viruses, serological typing with monoclonal antibodies showed an antigenic drift in this pathogenic strain. conventional 'intermediate' ibd vaccines are probably more antigenically related to the pathogenic strain than the mild ones and were effective in protecting spf chickens against ... | 1991 | 18680006 |
| effect of infectious bursal disease virus infection on the severity of aspergillus flavus aspergillosis of chickens. | two groups of broiler chickens were infected with infectious bursal disease virus (ibdv) and aspergillus flavus or a. flavus alone, respectively. loss in weight was 2.5 times more severe in the ibdv + a flavus birds than in those infected with a. flavus alone. mottling of liver with grey spots was observed only in the ibdv + a. flavus broilers. histopathological sections of the lungs showed more necrosis, granulomas and fibrosis in the ibdv + a. flavus group, and the fungus was reisolated for a ... | 1991 | 18680009 |
| infectious bursal disease virus type 1-induced suppression of chicken lymphocyte response to mitogen. | infectious bursal disease type 1 (ibdv-1) caused severe suppression of the proliferative response of normal chicken peripheral blood lymphocytes (pbl) to concanavalin a (con a). the factors capable of such suppression were not associated with virus particles and were stable at 56 degrees c for 1 h. their molecular weight ranged from under 100 kda to over 300 kda. they were not prostaglandin-like material, and their suppression of pbl was not due to interference with the con a action. | 1991 | 18680015 |
| health survey of backyard poultry and other avian species located within one mile of commercial california meat-turkey flocks. | a survey was conducted to characterize domestic and exotic bird populations, estimate seroprevalence to selected disease agents, and describe health management practices on 62 premises containing "backyard" flocks located within one mile of 22 commercial california meat-turkey flocks participating in national animal health monitoring system (nahms). chickens were present on 56 backyard premises and turkeys on seven. antibodies were identified against mycoplasma gallisepticum, m. synoviae, m. mel ... | 1991 | 1854324 |
| action spectrum of antiviral factor from chicken sera. | rous sarcoma virus infections of regressor line chickens stimulate the transient production of antiviral factors in the serum. earlier the present authors reported that a viral neutralization factor (vnf) inactivated rous sarcoma virus during a 3-h incubation. the vnf is likely to have a broad antiviral and antimicrobial spectrum because it is active against several unrelated pathogenic poultry viruses. the present study measured the activity of vnf against newcastle disease virus, infectious bu ... | 1991 | 1664518 |
| pathobiology of cryptosporidiosis (c. baileyi) in broiler chickens. | pathologic and clinicopathologic changes were examined in broiler chickens inoculated with cryptosporidium baileyi (cb) alone or in combination with infectious bronchitis virus (ibv), infectious bursal disease virus (ibdv) or escherichia coli (ec). concurrent infections with cb and either ibv or ec resulted in a greater respiratory inflammatory response than either agent given alone. concurrent cb, ibv or ec infections resulted in a decreased density of respiratory cryptosporidial stages. no int ... | 1991 | 1667932 |
| first report of an infectious bursal disease outbreak in a vaccinated chicken flock in anambra state, nigeria. | infectious bursal disease was reported in a flock of 7-week old vaccinated chickens. clinical findings and post-mortem changes were classical as well as the microscopic pathology of the bursa. bursal homogenates from dead birds were positive for ibd virus antigen in agar gel diffusion test (agdt). convalescent sera obtained from birds 14 days following the onset of clinical signs were also positive for ibd virus antibody in agdt. seven-week old susceptible birds, each infected i/m with 0.1 ml of ... | 1991 | 1668870 |
| sequence analysis of infectious pancreatic necrosis virus genome segment b and its encoded vp1 protein: a putative rna-dependent rna polymerase lacking the gly-asp-asp motif. | the genome segment b sequence of infectious pancreatic necrosis virus was determined for both the jasper and sp serotypes. the sequences are 2784 and 2630 bp long, respectively, and contain a single large open reading frame encoding the vp1 protein, the putative rna-dependent rna polymerase (rdrp) of ipnv. the proteins exhibit an 88% homology with each other, but only 41% with infectious bursal disease virus (ibdv) vp1, another member of the birnaviridae. despite the low overall homology between ... | 1991 | 1901682 |
| efficacy of coarse-spray administration of a reovirus vaccine in young chickens. | coarse-spray (cs) administration of a commercial s1133 reovirus vaccine in chickens for prevention of clinical viral tenosynovitis (vt) infection was evaluated. in expt. 1, one-day-old specific-pathogen-free (spf) white leghorns were vaccinated with a combination of reovirus, newcastle disease (nd), and infectious bronchitis (ib) vaccines by cs and infectious bursal disease vaccine by the subcutaneous (sq) route. in expt. 2, one-day-old commercial broilers were vaccinated by cs with reovirus vac ... | 1991 | 1851416 |
| isolation of infectious bursal disease virus from turkeys with arthritic and respiratory symptoms in commercial farms in quebec. | infectious bursal disease viruses (ibdvs) were isolated from turkeys showing symptoms of arthritis and respiratory disease in commercial poultry farms in the province of quebec, canada. synovial fluids collected from hock joints of arthritic birds and peripheral blood leukocytes obtained from the birds with respiratory problems were used for virus isolation in embryonated chicken eggs, and vero and bgm-70 cell cultures. the infected cells were evaluated for the presence of ibdv by indirect immun ... | 1991 | 1851419 |
| physicochemical and immunological characterization of recombinant host-protective antigen (vp2) of infectious bursal disease virus. | small fusions to the n-terminal end of the host-protective antigen (vp2) of infectious bursal disease virus lead to stable expression of vp2 in escherichia coli and yeast, and reduce the levels of inclusion body formation in e. coli in comparison to vp2 constructs with larger n-terminal fusions. vp2 produced with small n-terminal fusions, like native viral vp2, can be fractionated into a high molecular weight 'multimeric' form and a monomeric form. a virus-neutralizing monoclonal antibody that o ... | 1991 | 1759490 |
| comparison of neutralizing antigens of recent isolates of infections bursal disease virus. | monoclonal antibodies (mabs) to a local turkey isolate (qt-1) of infectious bursal disease virus (ibdv) were produced to identify the virus-specific neutralizing proteins. radioimmunoprecipitation assays showed that all the mabs were specific for major viral protein, vp2. two of the mabs neutralized the local turkey and chicken isolates along with a reference strain belonging to serotype 1 but not the reference strain of serotype 2. the reactivities of the neutralizing mabs against two reference ... | 1991 | 1850233 |
| immunosuppressive effect of infectious bursal disease virus strains of variable virulence for chickens. | infection of chicks with attenuated lp and sp clones of the rf-1 strain of infectious bursal disease virus was shown to exert no immunosuppressive effect, whereas the parent strain rf-1tc and the original virulent strain rf-1wt were immunosuppressive. one-day-old chicks infected with lp and sp clones showed no suppression of immunological response to live newcastle disease vaccines b1 and tcnd, and to bivalent infectious coryza vaccine. on the other hand, infection with rf-1tc or rf-1wt strains ... | 1991 | 1850895 |
| the effect of infectious bursal disease virus on b lymphocytes and bursal stromal components in specific pathogen-free (spf) white leghorn chickens. | the effect of infectious bursal disease virus (ibdv) was studied on adult specific pathogen-free (spf) white leghorn chickens through analysis of peripheral blood cell suspensions and histological staining patterns on various tissue types, with specific mabs. a rapid, progressive loss of b lymphocytes was observed in the bursal cortex and medulla, peripheral blood and thymic medulla. there was, however, a resistant population of mui-36+ cells at the bursal cortico-medullary junction and scattere ... | 1991 | 1663462 |
| immunosuppression induced by infectious bursal disease virus. | immunosuppression caused by infectious bursal disease virus (ibdv) is of major interest because of the widespread occurrence of the infection in commercial chickens. infection with ibdv at an early age significantly compromises the humoral and local immune responses of chickens. the cellular immune response is also compromised by apparently to a lesser extent and for a short period. the immunosuppression seems to be a result of direct effect (lysis) of b cells or their precursors. other mechanis ... | 1991 | 1664162 |
| a recombinant fowlpox virus that expresses the vp2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus. | the coding sequences of vp2 from a virulent strain, 52/70, of infectious bursal disease virus (ibdv) were excised from a cdna clone and inserted into a fowlpox plasmid insertion vector. the resulting plasmid, pibd 1, was used to construct a recombinant fowlpox virus, fpibd 1, which expressed vp 2 as a beta-galactosidase fusion protein. chickens vaccinated with fpibd 1 at 1 and 14 days of age, were challenged at 28 days with either ibdv strain 52/70 or the highly virulent strain cs 89. these chic ... | 1991 | 1659797 |
| [effect of viral structure and replication characteristics on the pathogenesis of infectious bursal disease]. | infectious bursal disease (ibd) is a highly contagious disease of young chickens, which occurs world-wide, and is responsible for severe losses in poultry industries. in birds surviving an acute infection, lymphoid cells in the bursa of fabricius are destroyed, resulting in b-cell-dependent immunodeficiency. this causes increased susceptibility to diseases by otherwise harmless agents. the decisive role in the pathogenesis of ibd is played by the bursa, representing the target organ of the aetio ... | 1991 | 1648347 |
| stable synthesis of viral protein 2 of infectious bursal disease virus in saccharomyces cerevisiae. | viral protein 2 (vp2) from infectious bursal disease virus and its precursor polyprotein (n-vp2-vp4-vp3-c), in the absence of their native n-terminal region (19 amino acids), required fusion of yeast presequences for their stable synthesis in saccharomyces cerevisiae [jagadish et al., gene 95 (1990) 179-186]. restoration of the missing 19 aa resulted in stable synthesis of vp2, indicating the significance of the n-terminal region in protein stability. | 1991 | 1660840 |
| ascites syndrome in spf light sussex chickens. | an ascites syndrome was induced in 17 to 28 per cent of specific pathogen-free (spf) light sussex (lsx) chickens given a low protein (16 per cent crude protein) high calcium (3.5 per cent calcium) layer crumble feed on two separate occasions 6 months apart. affected chickens had increased right ventricular weight as a proportion of either total heart weight or live-weight at 3 weeks of age, compared with non-affected lsx chickens on the same feed, thus indicating right ventricular hypertrophy. t ... | 1991 | 1663140 |
| sequence analysis and expression of the host-protective immunogen vp2 of a variant strain of infectious bursal disease virus which can circumvent vaccination with standard type i strains. | the host-protective antigen vp2 of a variant strain of infectious bursal disease virus (ibdv) which emerged from a vaccinated flock and is able to circumvent vaccination with classic type i strains of ibdv, was cloned and its nucleotide sequence determined. virus-neutralizing monoclonal antibodies (mabs) raised against the australian 002-73 strain of ibdv did not react or reacted only very weakly with the expression product of the variant virus. the deduced amino acid sequence of vp2 from the va ... | 1991 | 1651980 |
| double-antibody sandwich elisa for detection of infectious bursal disease virus. | a double-antibody sandwich elisa was employed for detection of ibd virus in bursal suspension. fifty ibd-free white leghorn chickens aged 5 weeks were experimentally infected with ibd virus. bursae were collected 4, 8, 12, 24, 36 and 48 hours and 3, 4 and 5 days post-infection. an equal number of chickens acted as appropriate controls. the colour difference between a positive and a negative reaction was clearly distinguished with the naked eye. the cut-off level between elisa negative and elisa ... | 1991 | 1652348 |
| internal initiation and frameshifting in infectious bursal disease virus sequence expressed in escherichia coli. | as part of our efforts to produce native molecules of the host protective antigen (vp2) of infectious bursal disease virus (ibdv) in microorganisms, we have restored nucleotide sequences encoding the vp2 n-terminus which were not present in the original clone. in addition to this process, we produced constructs that contained +5, +4, -1, and -2 frameshifts that still allowed expression of the ibdv polyprotein, vp2 and vp3. the size of translation products and examination of the nucleotide sequen ... | 1991 | 1653499 |
| technological advances in poultry vaccines. | the work described in this paper represents an extended program utilizing both traditional and novel technologies to develop a range of poultry vaccines which target current or emerging opportunities in domestic and international marketplaces. the successes with the traditional products to date have been gratifying and the advent of a series of recombinant vaccines over the next few years holds promise for the generation of a range of novel immunobiologicals. | 1991 | 1369351 |
| infectious bursal disease--b cell dependent immunodeficiency syndrome in chickens. | infectious bursal disease of chickens can run an acute lethal course, or death can result from a b cell-dependent immunodefect due to destruction of the bursa of fabricius following infection with infectious bursal disease virus (ibdv). this member of the birnaviridae has been characterized, the nucleotide sequence and coding capacity of the two genomic segments of dsrna has been determined, and the functional significance of the four structural proteins has been largely elucidated. the antigeni ... | 1991 | 1656933 |
| variability in a commercially available enzyme-linked immunosorbent assay system. i. assay variability. | three experiments were conducted to characterize the variation in enzyme-linked immunosorbent assay (elisa) kits for infectious bronchitis virus (ibv) and infectious bursal disease virus (ibdv). expt. 1 was carried out to determine the variation in assay results when the same pools of low-, medium-, and high-titered serum were assayed. significant variation occurred among separate lots and among test plates within the same lots for the ibv and ibdv assays. in most cases, variability between days ... | 1991 | 1649588 |
| immunogenicity of infectious bursal disease viruses in chickens. | cross-protective properties of infectious bursal disease viruses (ibdvs) were studied. viruses represented different subtypes of serotype 1, including recently isolated viruses (variants), and a serotype 2 virus. chickens were vaccinated at 3 weeks of age with inactivated vaccines containing 10(5), 10(6), 10(7), or 10(8) mean tissue-culture infectious dose of a given virus and challenged 2 weeks later using either 10(2) or 10(3.5) mean embryo infectious dose (eid50) of either a standard virus or ... | 1991 | 1659364 |
| variability in a commercially available enzyme-linked immunosorbent assay system. ii. laboratory variability. | an experiment was conducted to determine the amount of variability that occurred in enzyme-linked immunosorbent assays when samples from common serum pools were assayed in five different labs on three consecutive days. low- (approximately 1:2000), medium- (approximately 1:4000), and high-titered (approximately 1:8000) serum pools were distributed to five poultry industry laboratories that cooperated in the study. results varied significantly among different laboratories and among different days ... | 1991 | 1649589 |
| effect of infectious bursal disease virus vaccines on persistence and pathogenicity of modified live reovirus vaccines in chickens. | two commercially available live reovirus vaccines, alone or in combination with two infectious bursal disease virus (ibdv) vaccines, were evaluated for safety and efficacy in specific-pathogen-free leghorn chicks. four trials were conducted to evaluate the vaccine combinations. at periodic intervals during the trials, tissues were collected and assayed for residual reovirus and examined for histological changes. six weeks following reovirus vaccination, all treatment groups were challenged with ... | 1991 | 1851413 |
| genome cloning and analysis of the large rna segment (segment a) of a naturally avirulent serotype 2 infectious bursal disease virus. | the genome of infectious bursal disease virus (ibdv) of serotype 2 (strain oh) has been cloned, and 3171 nucleotides of genome segment a cdna sequence have been determined for the first time. sequence homology of oh-ibdv with the most distant serotype 1 ibdv at the nucleotide level is 83.1%, and the amino acid sequence homology of the polyprotein is 89.6%. alignment of the polyprotein amino acid sequences showed the hypervariable region in vp2 to be 151-152 amino acid residues long in ibdv. a se ... | 1991 | 1651602 |
| determination of infectious bursal disease virus titration and neutralization endpoints using fluorogenic staining. | an automated method for determining infectious bursal disease virus (ibdv) titration and neutralization endpoints is described. the method employs the fluorogenic ester carboxyfluorescein-diacetate (cfda) to stain cell monolayers in 96-well plates and a fluorescence-concentration analyzer. titration results are compared with immunofluorescence and plaque assay titers. virus-neutralization endpoint determination is objective, and the endpoints of replicate tests were equivalent or within one dilu ... | 1991 | 1659366 |
| the structural polypeptide vp3 of infectious bursal disease virus carries group- and serotype-specific epitopes. | two independent non-overlapping epitopes could be demonstrated on the structural protein vp3 of infectious bursal disease virus by non-neutralizing monoclonal antibodies produced against serotypes i and ii. both serotypes have one epitope in common, whereas the second epitope is distinct for serotype i and serotype ii. | 1991 | 1716656 |
| virus-neutralizing and passively protective monoclonal antibodies to infectious bursal disease virus of chickens. | murine monoclonal antibodies (mabs) were produced to assist in the identification and characterization of the virus-neutralizing epitopes of infectious bursal disease virus (ibdv). only mabs that reacted in western blotting with viral protein 2 (vp2) or immunoprecipitated vp2 neutralized the infectivity of the virus in cell culture and passively protected young chickens from infection. three of the neutralizing mabs did not react with denatured viral proteins. additivity enzyme-linked immunosorb ... | 1991 | 1713030 |
| heterogeneity of the antigenic site responsible for the induction of neutralizing antibodies in infectious bursal disease virus. | using neutralizing monoclonal antibodies, three categories of escape mutants were selected from a stock of wild-type infectious bursal disease virus (ibdv). additional mutants were found, where alterations coexisted in two or three of these epitopes. although each group of mutants had a distinct reaction pattern with neutralizing monoclonal antibodies, all types of mutants were neutralized by convalescent chicken sera to the same extent. in spite of the lack of homogeneity in these antigenic sit ... | 1991 | 1715158 |
| localization of a vp3 epitope of infectious bursal disease virus. | an immunodominant region of vp3, one of the two structural proteins of infectious bursal disease virus (ibdv strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in escherichia coli, followed by immunoblot analysis of the synthesized products. the epitope located within 58 amino acids reacted very strongly with a mouse monoclonal antibody (mab 17/80) raised against ibdv 002-73. this immunodominant region may be useful in serodiagnosis of ibdv infection in ... | 1991 | 1716030 |
| subclinical infectious bursal disease in an integrated broiler production operation. | a field study was designed to determine the prevalence of subclinical infectious bursal disease (ibd) in broiler chickens from a commercial poultry company. bursae of fabricius (bf) from two vaccinated and three nonvaccinated broiler flocks were evaluated histologically, and antibody profiles of these broiler and matched parent breeder flocks were established. lesions of ibd, including lymphoid necrosis, stromal edema, and infiltrates of heterophils and macrophages, were first detected in bf at ... | 1992 | 1333817 |
| detection of infectious bursal disease virus in digested formalin-fixed paraffin-embedded tissue sections by polymerase chain reaction. | 1992 | 1333820 | |
| naturally occurring-neutralizing monoclonal antibody escape variants define the epidemiology of infectious bursal disease viruses in the united states. | a panel of two non-neutralizing and six neutralizing monoclonal antibodies (mabs) were used in antigen-capture enzyme immunoassays (ac-elisa) to examine the antigenicity of 1301 wild type infectious bursal disease viruses (ibdv) isolated from different poultry flocks throughout the united states over a three year period. analysis of these isolates with protective, neutralizing mabs directed against the vp2 structural protein of ibdv showed that four antigenically distinct groups of serotype 1 ib ... | 1992 | 1333761 |
| search for the origin of multiple sclerosis by first identifying the vector. | by intensive study of environmental conditions during the final stages in an active cluster area, an attempt is made to crack the enigmatic deadlock concerning the origin and etiological cause of multiple sclerosis (ms). previous investigations of other cluster areas have emphasized the probability that the outbreaks of ms were most likely caused by an unknown exogenous environmental agent, but unfortunately, the studies were conducted several years after the epidemics had occurred, making it vi ... | 1992 | 1316536 |
| structural proteins of classic and variant strains of infectious bursal disease viruses. | structural polypeptides of six tissue-culture-origin (bgm-70 continuous cell line) infectious bursal disease viruses representing classic and variant strains of serotype 1 and one serotype 2 strain were analyzed and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. additionally, two of the variant strains were propagated in vivo in bursa of fabricius and compared with those grown in cell culture. differences among the structural proteins of serotype 1 viruses were minor and ... | 1992 | 1336659 |
| infectious bursal disease of poultry: antigenic structure of the virus and control. | the present knowledge of genome organisation, structural basis of pathogenicity and antigenicity of infectious bursal disease virus (ibdv) are briefly reviewed. the current situation of ibdv infection in various countries is stated and recommendations for improved vaccination schemes are given. | 1992 | 1336239 |
| response of b congenic chickens to infection with infectious bursal disease virus. | six congenic lines of chickens that differ from the parental inbred line rprl-15i5 for genes in the major histocompatibility (b) complex were used to study the influence of the b haplotypes on the response of chickens to infection with virulent infectious bursal disease virus (ibdv) at 1 day or 4 weeks of age, and on the antibody response to vaccination with live or inactivated oil-emulsion (oe) ibdv vaccines at 7 weeks of age. ibdv-induced immunodepression and lesions in the bursa, spleen, and ... | 1992 | 1336660 |
| immunosuppressive effect of a highly virulent infectious bursal disease virus isolated in japan. | the immunosuppressive effect of infectious bursal disease virus (ibdv) on vaccination against newcastle disease (nd) was compared among 2-, 3-, and 4-week-old chickens inoculated with the highly virulent ibdv field isolate 90-11 and the reference serotype 1 strain gbf-1. in all age groups, isolate 90-11 severely suppressed antibody response to nd vaccination and protective vaccinal immunity against nd. in contrast, chickens inoculated with strain gbf-1 and vaccinated with nd vaccine were well pr ... | 1992 | 1336662 |
| isolation of chicken anaemia virus from broiler chickens. | chicken anaemia virus (cav) infection was demonstrated, by both serology and virus isolation, in 1- to 6-week-old broiler chickens originated from various parent flocks in hungary. total losses in the broiler flocks were estimated at 7 to 8% and about 25% of the chickens failed to reach target body mass by the 7th week of life. the clinical signs, postmortem lesions and histopathological changes of the affected chickens were similar to those of naturally occurring cav-induced infectious anaemia ... | 1992 | 1298167 |
| relationship of common avian pathogen antibody titers in so-called chicken anemia agent (caa)-antibody-positive chicks to titers in caa-antibody-negative chicks. | antibody titers for infectious bursal disease virus (ibdv), infectious bronchitis virus, newcastle disease virus, and reovirus from chicks with chicken anemia agent (caa) antibodies were compared with antibody titers from their caa-antibody-negative counterparts. these comparisons were made in 396 chickens that were 1 day, 2 weeks, 8-9 weeks, 10 weeks, 17 weeks, or 29-32 weeks old. only one serum sample was collected from any given chick or chicken. there were no significant differences between ... | 1992 | 1320864 |
| characterization of nonradioactive hybridization probes for detecting infectious bursal disease virus. | reverse transcription followed by the polymerase chain reaction was used to amplify a fragment of infectious bursal disease virus (ibdv) strain p3009 genome. the amplified dna fragment was annealed into the plasmid puc18 and used to transform escherichia coli strain jm109. a clone that contained ibdv-specific nucleotide sequences was selected and designated pc23. the dna fragment within pc23 was 320 base pairs in length and designated c23. radiolabeled probes prepared from c23 hybridized to geno ... | 1992 | 1322934 |
| susceptibility of chicken monocytic cell lines to infectious bursal disease virus. | 1992 | 1322719 | |
| genetic mechanisms of antigenic variation in infectious bursal disease virus: analysis of a naturally occurring variant virus. | the major immunogenic protein vp2 from a pathogenic field isolate (variant a virus) of infectious bursal disease virus (ibdv) was cloned and sequenced to examine antigenic variations. the vp2 open reading frame consists of 1509 nucleotides and codes for a 503 amino acid protein. overall, the vp2 amino acid sequence of the variant a virus shares 98.6% identity with vp2 genes from other published ibdv strains. however, within the central region of vp2 (amino acids 222-334) lies a highly divergent ... | 1992 | 1329340 |
| occurrence of acute infectious bursal disease with high mortality in japan and pathogenicity of field isolates in specific-pathogen-free chickens. | highly virulent infectious bursal disease virus (ibdv) was isolated from field cases, and the pathogenicity of the isolates was examined in specific-pathogen-free chickens. chickens inoculated with the isolates developed severe clinical disease with a high mortality rate. histopathologically, infectious bursal disease was characterized by bursal and thymic necrosis, aplastic anemia, acute hepatitis with fatty change, and systemic inflammatory response. in addition to functional abnormalities in ... | 1992 | 1329709 |
| pathogenicity and preliminary antigenic characterization of six infectious bursal disease virus strains isolated in france from acute outbreaks. | six isolates originating from acute outbreaks of infectious bursal disease recently reported in broiler and pullet flocks in france were studied with respect to their pathogenicity and their antigenic relatedness to the faragher 52/70 reference strain. although the mortality experimentally induced in susceptible chickens by the field strains was sometimes four times higher than that which followed the inoculation of the reference strain (16 to 48% versus 12%), neither mortality nor morbidity wer ... | 1992 | 1337234 |
| prevalence of subclinical infectious bursal disease and its significance in india. | from a total of 32 poultry flocks, 1,176 serum samples were screened by the agar gel precipitation test and 314 (26.7%) were positive for antibodies to infectious bursal disease virus (ibdv). prevalence of infection on the farms ranged from 8.88 to 53.84 per cent. the prevalence was highest (61.82%) in chickens between 7 and 11 weeks old and lowest (3.92%) in those above 22 weeks of age. in commercial broilers and layers 51.61 and 17.78% respectively were seropositive reactors. the high prevalen ... | 1992 | 1339037 |
| investigations of environmental conditions during cluster indicate probable vectors of unknown exogenous agent(s) of multiple sclerosis. | during the tail-end of an active cluster several environmental investigations indicated that wildbirds were very probably the vectors of the unknown exogenous agent of ms. canine distemper and genetic-autoimmune theories were very definitely eliminated because of the unusual pattern of the cluster. studies of several avian pathogens unveiled marek's (mdv) and/or ibd (gumboro) as the most likely candidates for exogenous agent of ms. | 1992 | 1312422 |
| immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus. i. kinetic alterations of avian lymphocyte subpopulations. | the potential effect of chicken anemia agent (caa) alone or in combination with infectious bursal disease virus (ibdv) on the immune system of young chickens was determined by measuring alterations in hematocrit values, lymphoid organ-to-body weight ratios and lymphoid cell concentrations at 4, 7, 10, 14, 17, 21, 28 and 42 days post-inoculation (pi). lymphocyte subpopulations were identified and counted by flow cytometry using cell suspensions stained with monoclonal antibodies (mabs) for panlym ... | 1992 | 1333676 |
| ultrastructural studies on interaction of infectious bursal disease virus (ibdv) and aflatoxin b1 on chick embryo fibroblast cell culture. | light and electron microscopic evaluation of chick embryo fibroblast (cef) cell culture inoculated with graded doses (0.25, 2.5 and 25 micrograms/ml medium) of aflatoxin b1 with and without infectious bursal disease virus (ibdv) was undertaken. the light microscopy revealed degeneration, detachment and necrosis of fibroblasts and multiple plaques formation in ibdv infected group without and with (0.25, 2.5 micrograms) aflatoxin b1. the cultures infected with virus, with or without 25 micrograms ... | 1992 | 1334042 |
| detection of infectious bursal disease virus infection using the polymerase chain reaction. | the method of reverse transcription (rt) followed by the polymerase chain reaction (pcr) was used to amplify two different fragments of the infectious bursal disease virus (ibdv) genome. two sets of primer framed two different regions within the genes coding for proteins vp2 and vp3, respectively. both sequences were detected in five strains of ibdv, whereas, none were obtained from uninfected control cells. the sensitivity of rt-pcr was carried out on nucleic acids from the ibdv infected cell c ... | 1992 | 1335456 |
| molecular detection of infectious bursal disease virus by polymerase chain reaction. | the polymerase chain reaction (pcr) technique was applied to the detection of infectious bursal disease virus (ibdv). reverse transcription followed by the pcr was used to amplify a portion of ibdv genome. a set of primers that specify a 150-base-pair segment of ibdv genome was chosen from an australian strain of ibdv. standard challenge strain and variant strains a, d, e, g, and gls-5 of ibdv serotype 1 and oh strain of serotype 2 from infected bursae were subjected to reverse transcription, fo ... | 1992 | 1320861 |
| isolation and propagation of infectious bursal disease virus using the ovine kidney continuous cell line. | twenty-six samples known to contain infectious bursal disease virus (ibdv) were examined by virus-isolation attempts on ovine kidney (ok) cell line, vero cell line, and chicken embryo fibroblast (cef) cultures. virus was isolated from two of 26 samples, three of 26 samples, and three of 25 samples on ok, vero, and cef cultures, respectively. however, in contrast to ibdv replication in vero and cef cultures, isolated virus was unable to induce serially sustained cytopathic effects (cpe) during su ... | 1992 | 1320862 |
| immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus. ii. alterations of in vitro lymphoproliferation and in vivo immune responses. | to determine the functional impact of alterations in lymphocyte concentrations and ratios following infection with chicken anemia agent (caa) alone or in combination with infectious bursal disease virus (ibdv) on the immune system of young chickens, in vitro lymphoproliferation assays and in vivo responses to vaccination with several common viral agents were assessed at various time intervals post-inoculation (pi). concanavalin a (con a), phytohemagglutinin (pha) and pokeweed mitogen (pwm) stimu ... | 1992 | 1333677 |
| tissue-print hybridization using a non-radioactive probe for the detection of infectious bursal disease virus. | tissue-print hybridization was evaluated as a simplified means for detection of infectious bursal disease virus (ibdv) in the bursa of fabricius from infected chickens. the assay employed a biotin-labeled synthetic oligonucleotide as a probe. the bound probe was detected using a color assay consisting of streptavidin conjugated to alkaline phosphatase. bursae were imprinted onto nitrocellulose and then hybridized with the biotinylated probe. bursal prints from ibdv-infected chickens were readily ... | 1992 | 1320859 |
| digoxigenin-labeled nucleic acid probe for the detection of infectious bursal disease virus in infected cells. | a cdna probe was synthesized from the vp-4 region of a virulent field isolate of infectious bursal disease virus (ibdv). the probe was labeled during synthesis with a non-radioactive steroid hapten, digoxigenin. the probe was used to develop a hybridization assay to detect the presence of ibdv in infected cell-culture and tissue suspensions from the bursa of fabricius of infected chickens. the test was rapid, reproducible, and sensitive, and it could detect four serologic subtypes of ibdv, inclu ... | 1992 | 1320860 |
| analysis of inter-serotypic structural relationships of infectious bursal disease virus using detergent solubilization and radioimmunoprecipitation with monoclonal antibodies. | monoclonal antibodies (mabs) neutralizing only the infectious bursal disease virus strains (ibdv) belonging to serotype 1 also immunoprecipitated the heterologous major antigenic proteins of serotype 2 ibdv. detergent-solubilization followed by radioimmunoprecipitation assays (ripa) using the mabs revealed structural similarities between the conformation-dependent antigenic determinants of ibdv of the two existing serotypes. the presence of non-ionic detergent triton x-100 determined the binding ... | 1992 | 1374071 |
| safety of infectious bursal disease vaccines: assessment of an acceptability threshold. | this study was undertaken to check the safety of commercially available infectious bursal disease (ibd) vaccines in terms of bursal damage, and their immunodepressive effects as evaluated by testing the birds after vaccination for their response to newcastle disease vaccination. further requirements are proposed to establish a suitable safety standard. | 1992 | 1337527 |
| effect of cell-culture passage on the pathogenicity and immunogenicity of two variant strains of infectious bursal disease virus. | two variant strains of infectious bursal disease virus, in and e, were adapted and passaged in an established cell line (bgm-70) 30 times and 40 times, respectively. passage in cell culture resulted in loss of pathogenicity. however, both viruses maintained their antigenicity and immunogenicity, as demonstrated by the immunofluorescence and virus-neutralization tests and by the satisfactory protection induced by vaccinating specific-pathogen-free (spf) chickens with inactivated preparations of b ... | 1992 | 1320871 |
| biological roles of the major capsid proteins and relationships between the two existing serotypes of infectious bursal disease virus. | neutralizing monoclonal antibodies (n-mabs) were produced against infectious bursal disease virus (ibdv) of serotypes 1 and 2. the n-mabs recognizing the major antigenic proteins vp2 and vp3, were characterized using different strains of ibdv representing the existing two serotypes and a variant subtype of serotype 1. the biological properties of these viral antigens as defined by the mabs in vitro, were studied utilizing post-adsorption virus neutralization tests and fluorescence-activated cell ... | 1992 | 1333752 |
| use of polymerase chain reaction for efficient cloning of dsrna segments of infectious bursal disease virus. | a method is described for efficient cloning of the large rna segment of infectious bursal disease virus (ibdv) after reverse transcription and cdna amplification. complementary dna segments of ibdv were prepared using reverse transcriptase and specific primers homologous to the conserved region at the 3' end of the ibdv sequence. the resulting cdna segments were amplified using taq dna polymerase and a pair of specific primers. three separate primer pairs were used for amplification, each yieldi ... | 1992 | 1329714 |
| differential detection of infectious bursal disease virus serotypes, using cdna probes to vp2 coding region. | two nonoverlapping clones, poh405 and poh632, containing cdna inserts in the vp2 coding region of genome segment a were selected from a cdna library prepared from the double-stranded rna genome of the oh strain of infectious bursal disease virus (ibdv) of serotype 2. clone poh405, which is located in the hypervariable segment of vp2, is 328 base pairs long, has nucleotide sequence homology of 72 to 73%, and amino acid sequence homology of 64 to 67% with ibdv strains of serotype 1. clone poh632, ... | 1992 | 1324628 |
| antigen dependent adjuvant activity of a polydispersed beta-(1,4)-linked acetylated mannan (acemannan). | the adjuvant properties of a polydispersed beta-(1,4)-linked acetylated mannan, acemannan (ace-m), were evaluated. day-old broiler chicks were randomly selected and allocated to four flocks (vac 1-4). the vac 1 flock was sham vaccinated with saline. the vac 2 flock was vaccinated with an oil-based vaccine (breedervac iii; newcastle disease virus (ndv), infectious bursal disease virus (ibdv) and infectious bronchitis virus). the vac 3 flock was vaccinated with a vaccine-ace-m mixture, and the vac ... | 1992 | 1320308 |
| virus disease as a consequence of viral pathogenicity and the anti-viral immune response. | the brief description of two virus systems, influenza and infectious bursal disease, shows enigmatically how at least two requirements must be met to render a virus pathogenic: the array of the whole genome rather than the formation of a particular "pathogenicity gene" and the capacity of the host cell to provide the appropriate microenvironment for an optimal posttranslational processing of structural proteins. in the case of influenza viruses this relates particularly to the cleavability of th ... | 1992 | 1326273 |
| detection of infectious bursal disease viruses using in situ hybridization and non-radioactive probes. | the in situ hybridization assay was developed for the detection of infectious bursal disease virus (ibdv) infections in chickens. bursal tissue samples were harvested 4 days following infection with the st-c, md, e, in, or sal ibdv strain. the cdna clones stc-243, located on genome segment a, and stc-119, located on genome segment b, were used to prepare non-radioactive probes. probes were labeled with digoxigenin and detected the homologous st-c virus and also heterologous viruses in bursal tis ... | 1992 | 1314553 |
| a monoclonal antibody capture enzyme-linked immunosorbent assay for detecting antibodies to infectious bursal disease virus. | a monoclonal antibody capture enzyme-linked immunosorbent assay (mab-elisa) for antibodies to infectious bursal disease virus (ibdv) in chicken sera was developed and compared with conventional elisa. when sera from farm chickens were tested by the two elisas and serum neutralization (sn), the correlation rate between sn and mab-elisa was 100% (49/49), and that between sn and conventional elisa was 81.6% (40/49). in mab-elisa, all of the sera that were antibody-negative by sn had low absorbance ... | 1992 | 1313039 |
| [evaluation of the results of elisa in the quantitative determination of antibodies against infectious bursitis in poultry]. | specific antibodies were investigated in serums of chicks vaccinated with live vaccine and revaccinated with inactivated vaccine against the infectious bursal disease virus, using three methods. an elisa technique was used to determine antibody titres at a fourfold serum dilution; the reaction was evaluated visually. the results were compared with the titres of neutralizing antibodies and percentage of samples with precipitin activity (fig. 1). the average values of neutralizing antibody titres ... | 1992 | 1321527 |
| isolation of virulent infectious bursal disease virus from field outbreaks with high mortality in japan. | 1992 | 1313701 | |
| recent advances in avian virology. | selected, recent research on the following avian diseases, and their causative viruses, has been reviewed: chicken anaemia, infectious bursal disease, turkey rhinotracheitis, avian nephritis, fowlpox, influenza, infectious bronchitis and turkey enteritis. | 1992 | 1319788 |
| use of an inactivated infectious bursal disease oil emulsion vaccine in commercial layer chicks. | commercial layer chicks of different ages were inoculated with either one-fifth, two-fifths or a full dose of an inactivated infectious bursal disease oil emulsion vaccine and then challenged with virulent virus. the partial doses given at seven or 10 days old gave only partial protection. a full dose given at 10, 14 or 28 days old failed to give full protection but a full dose administered at seven days old protected all the chicks after each challenge. | 1992 | 1311882 |
| the immunoglobulin m response in chicken serum to infectious bursal disease virus. | despite the severe bursal damage observed in 5-week-old chicks as a result of the replication of a virulent strain of infectious bursal disease virus, the virus-specific antibody response in serum followed a typical pattern. three methods were used to monitor the igm response, an indirect elisa using serum and elisas, carried out on gel chromatography fractions and eluants from affinity chromatography of serum. the profile of igm production was found to be similar by each method. possible future ... | 1992 | 18670968 |
| the use of monoclonal antibody probes for the detection of infectious bursal disease virus antigens. | two monoclonal antibodies (mab), 2e6 and 2g10, were used against infectious bursal disease virus (ibvd) p3009 in an immuno-dot assay to detect ibdv antigens from cell culture, and from bursa and spleen tissue samples of chickens. the limit of viral antigens detected by using both mab probes was 48 ng. the probes were used to detect five serotype 1 ibdv isolates and one serotype 2 ibdv strain. the result indicated that these probes had broad specificity. the probes, however, did not cross-react w ... | 1992 | 18670918 |
| a monoclonal antibody-based agar gel precipitin test for antigenic assessment of infectious bursal disease viruses. | five monoclonal antibodies (mabs) directed at different neutralization epitopes of infectious bursal disease virus (ibdv) precipitated ibdv antigens in double immunodiffusion. based on the positive or negative reaction of some of the mabs in agar gel precipitin tests (agpt), four different antigenic species of ibdv could be discerned. although the antigen-capture enzyme-linked immunosorbent assays (ac-elisa) were more sensitive than agpts for differentiating ibdv types, use of the mabs in agpt d ... | 1992 | 18670926 |
| pathogenesis of infectious bursal disease in cyclophosphamide-treated chickens. | broiler chickens inoculated with cyctophosphamide showed atrophic bursae and severe immuno-suppression. infectious bursal disease virus (ibdv) inoculation of the birds at 5 weeks of age caused neither clinical signs nor gross lesions. ibdv was re-isolated from some bursae samples. cyclophosphamide non-treated (cynt) chickens inoculated with ibdv showed bursal enlargement followed by atrophy. examination of sections of the organ showed severe lymphocytic necrosis and depletion. ibdv was re-isolat ... | 1992 | 18670979 |
| evaluation of vp2 epitopes of infectious bursal disease virus using in vitro expression and radioimmunoprecipitation. | a clone (pv 17-7) spanning a portion of the vp2 gene of infectious bursal disease virus (ibdv) was selected from a cdna library produced using the variant a virus strain. this clone was expressed in vitro and the protein products were immunoprecipitated with various virus-neutralizing antisera made against 6 different strains of ibdv. the antisera made against 4 variant strains immunoprecipitated the translation products from the pv 17-7 clone, but the antisera to the classic stc virus and the s ... | 1993 | 7679573 |
| the genetic basis for the antigenicity of the vp2 protein of the infectious bursal disease virus. | the genomic region coding for the antigenic structure responsible for the induction of neutralizing antibodies was localized in the central variable region of the vp2 gene by comparing the nucleotide sequence of five escape mutants derived from the standard infectious bursal disease virus strain cu-1. exchange of a single amino acid at one of the prominent hydrophilic parts of this region proved to be sufficient for altering the neutralizing properties. the reactivity of neutralizing antibodies ... | 1993 | 7688411 |
| a rapid quantitative method for detecting infectious bursal disease virus using polystyrene latex microspheres. | a monoclonal antibody (mab) to infectious bursal disease virus (ibdv) was bound to polystyrene latex microspheres. the microspheres agglutinated with extracts of bursae and sera from chickens infected with all strains or isolates of ibdv tested. agglutination appeared within a 10-min reaction time. the assay could detect a 10(3.7) to 10(4.5) mean embryo infective dose (eid50) of the virus in 0.01 ml and the titer of the assay was 10- to 40-times higher than that of the agar gel precipitin test. | 1993 | 8395538 |
| infectious bursal disease virus structural protein vp2 expressed by a fowlpox virus recombinant confers protection against disease in chickens. | two fowlpox virus recombinants were constructed which expressed the host-protective antigen, vp2, of infectious bursal disease virus (ibdv). recombinant fpv-vp 2.4.3 contained the gene for the vp 2-vp4-vp3 polyprotein under the control of the vaccinia virus late promoter p.l 11 inserted within the thymidine kinase (tk) gene of fpv. in infected chicken embryo skin (ces) cells vp2 and vp3 proteins were correctly processed from the polyprotein precursor molecule. recombinant fpv-vp2 contained only ... | 1993 | 8394069 |
| rapid and quantitative assay system for measuring anti-infectious bursal disease virus antibody using monoclonal antibody bound to polystyrene latex microspheres. | a monoclonal antibody (mab) to infectious bursal disease virus (ibdv) that has virus-neutralizing activity was bound to polystyrene latex microspheres the microspheres agglutinated with extracts of bursae from chickens infected with ibdv. agglutination was inhibited in a competitive manner by adding serum obtained from ibdv-infected chickens. the level of agglutination-inhibition depended on the serum antibody titer against ibdv. the reaction was visually accomplished within 5 minutes. the titer ... | 1993 | 8257372 |
| infectious bursal disease virus structural proteins expressed in a baculovirus recombinant confer protection in chickens. | plasmids were prepared that contained cdna segments of the large genomic segment a of infectious bursal disease virus (ibdv) strain gls-5. the genes encoding the ibdv structural proteins (vp2, vp3 and vp4) were introduced into the baculovirus transfer vector pbluebaci to obtain a recombinant baculovirus vibd-7. when insect cells were infected with recombinant viruses, the result was synthesis of ibdv precursor proteins which were processed by the viral protease. the recombinant ibdv antigens wer ... | 1993 | 8389805 |
| expression and rna dependent rna polymerase activity of birnavirus vp1 protein in bacteria and yeast. | birnaviruses typically encode a polyprotein that is the precursor to the structural proteins of the virus and a protein of rare abundance, vp1, that is the putative dsrna replicase and/or transcriptase. we have reconstructed the vp1 gene of ibdv from a library of cdna clones and expressed the gene in escherichia coli and in saccharomyces cerevisiae. we could not detect an rna polymerase activity associated with e. coli-derived vp1, and neither could we promote the yeast-derived vp1 to replicate ... | 1993 | 8220261 |
| studies on antigenic relatedness of classic and variant strains of infectious bursal disease viruses. | antigenic relatedness of six classic and variant strains of serotype 1 infectious bursal disease virus (ibdv) and one serotype 2 ibdv was investigated by western blotting and enzyme-linked immunosorbent assay (elisa) using polyclonal, monoclonal, and monospecific antibodies to single viral proteins (vp2 and vp3). all virus strains cross-reacted similarly, and the viruses were not distinguishable from each other by elisa or western blot analysis performed with polyclonal or non-neutralizing monoc ... | 1993 | 8257353 |
| comparative pathogenicity and serogrouping of three washington isolates of infectious bursal disease virus. | the pathology of three infectious bursal disease virus (ibdv) isolates of washington poultry origin (wa-678, wa-770, and wa-994) and seven other known ibdv strains (sal, d-78, mo, oh, var-a, 2512, and im) was studied in 3-week-old specific-pathogen-free chickens. inoculation with im and 2512 strains resulted in illness and death. no clinical signs or mortality were present with wa-678, wa-770, and wa-994. macroscopically, bursae were swollen and gelatinous with occasional hemorrhages. isolate wa ... | 1993 | 8257354 |
| presence of lesions without virus replication in the thymus of chickens exposed to infectious bursal disease virus. | specific-pathogen-free (spf) chickens were exposed to the im and va isolates of virulent infectious bursal disease virus (ibdv). both viruses induced rapidly progressing lymphoid cell depletion in the bursa. the bursal lesions persisted through the observation period of 16 days. the virus-exposed birds also had histologic lesions in the thymus. thymic lesions peaked at 3-4 days postinoculation (pi) and then subsided. immunofluorescence (if) and antigen-capture enzyme-linked immunosorbent assay ( ... | 1993 | 8257365 |
| antibody detection in matched chicken sera and egg-yolk samples by commercial enzyme-linked immunosorbent assay kits for newcastle disease virus, infectious bronchitis virus, infectious bursal disease virus, and avian reovirus. | elisa kits have been used to detect antibody in egg yolk. the major advantage eggs offer over blood samples is the ability to collect samples without compromising flock biosecurity. a disadvantage to using egg yolk over sera concerns the method of preparing yolk for antibody testing. the technique used in this study involved a simple dilution method with no mixing or extraction. to determine the adequacy of yolk samples to replace serum samples, a serum sample and the first six eggs were obtaine ... | 1993 | 8257378 |
| flow cytometric analysis of the neutralizing immune response against infectious bursal disease virus using reticuloendotheliosis virus-transformed lymphoblastoid cell lines. | infectious bursal disease virus (ibdv) is a lymphotropic virus with cytocidal effect on b lymphocytes of the bursa of fabricius. we investigated the susceptibility of clonal populations of reticuloendotheliosis virus-transformed chicken b lymphocytes of both spleen and bursal origin to ibdv infection. the infected cells were metabolically-labelled and the viral polypeptides were analyzed by immunoprecipitation using monoclonal antibodies (mabs). virus adsorption and the effects of neutralizing c ... | 1993 | 8263113 |
| agar gel precipitin line patterns and pathogenicity of infectious bursal disease viruses. | twenty-nine strains of infectious bursal disease virus could be classified into three groups by the agar gel precipitin line patterns using two representative base antigens of f539 and g691 strains. the precipitin line of the first group (16 strains including f539) did not fuse with that of g691 base antigen and spur was seen. the line of the second group (2 strains) did not fuse with those of both base antigens. the line of the third group (11 strains including g691) did not fuse with that of f ... | 1993 | 8384895 |
| genetic differences in susceptibility of chicken lines to infection with infectious bursal disease virus. | mortality rates in 11 inbred and partially inbred chicken lines inoculated with a very virulent strain (cs89) of infectious bursal disease virus (ibdv) varied considerably, being highest (almost 80%) in a brown leghorn line (brl). bursa of fabricius to body weight ratios were depressed in the survivors in each line, but no differences were observed between lines. however, histological examination of bursae from survivors showed that, although bursal damage occurred in every line, it was most sev ... | 1993 | 8385328 |
| immunomodulating effects of levamisole in chicks immunocompromised by infectious bursal disease virus. | immunomodulating effects of levamisole in experimentally ibd induced immunosuppressed 7-days old white leghorn chicks have been observed. for this, infectious bursal disease (ibd) virus (poona strain) was used. all the chicks were immunised with sheep red blood cells to monitor antibody responses. a group of chicks each from infected (ps-l) and uninfected (pbs-l) groups were given 4 injections of levamisole hydrochloride at the daily dose of 1.5 mg per 100 g body weight starting from the second ... | 1993 | 8385372 |
| chemiluminescent detection of infectious bursal disease virus with a pcr-generated nonradiolabeled probe. | a polymerase chain reaction (pcr)-generated digoxigenin-labeled nonradioactive oligonucleotide probe was developed and utilized in slot-blot hybridization coupled with chemiluminescence for the detection of infectious bursal disease virus (ibdv). the probe was prepared from the rna of the standard challenge strain (stc) of ibdv serotype 1 by reverse transcription followed by 2 pcr amplifications with 2 separate sets of primers. rna of stc viruses prepared from bursae infected with stc viruses wa ... | 1993 | 8389597 |
| [serologic monitoring of pullet and laying hen flocks in switzerland: results from the years 1990 and 1991]. | in 1990 and 1991 4522 blood samples from 398 pullet flocks and 1338 blood samples from 128 laying flocks were monitored for antibody against infectious bronchitis virus, infectious laryngotracheitis virus, adenovirus, reovirus, infectious bursal disease virus, newcastle disease virus, mycoplasma gallisepticum and mycoplasma synoviae. the results are discussed for pullets and laying hens. | 1993 | 8211056 |
| seroprevalence of infectious bursal disease (ibd) in parts of tamil nadu, india. | sera samples of 400 apparently healthy broiler poultry from 23 commercial farms and 120 sera samples from commercial layer poultry [with specific lesions of infectious bursal disease (ibd) in the internal organs] were screened for the presence of ibd serotypes i, ii and the variant strain by the agar gel immuno diffusion (agid) test, employing standard antigens. likewise the bursae, spleen and kidney specimen from 96 layer poultry with lesions suggestive of ibd were screened for the presence of ... | 1993 | 8403839 |
| production and characterization of monoclonal antibodies against variant a infectious bursal disease virus. | monoclonal antibodies (mabs) were produced against a variant subtype of infectious bursal disease virus (ibdv) from delaware variant isolate a (var-a). splenocytes from immunized mice were fused to myeloma cells, and antibody-producing hybridomas were screened by dot-blot enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence (if) against the homologous isolate. specificity of the mabs was tested against viral isolates representing all six serologic subtypes of ibdv and three ... | 1993 | 8395799 |