Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| quantitation of antibody to non-hemagglutinating viruses by single radial hemolysis: serological test for human coronaviruses. | a single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. with the human coronaviruses as models, this test utilizes the binding properties of the chromic cation to attach viruses to glutaraldehyde-treated sheep erythrocytes. the most satisfactory system consisted of stabilizing washed sheep erythrocytes with 0.0073% glutaraldehyde for 15 min at 23 degrees c, binding a high concentration of virus to a 25% erythrocyte suspension with 0.001 ... | 1977 | 195976 |
| solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody. | an indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. the test involved ethanol fixation of microcultures of bovine rotavirus-infected bsc-1 cells and reacetion with bovine antirotavirus serum, followed by 125i-labeled rabbit anti-bovine immunoglobulin g. the technique was shown to be virus specific and highly sensitive. the fixed microcultures could be stored at 4 degrees c for at least 2 months without affecting the sensitivity of the test. th ... | 1977 | 195982 |
| enterotoxigenic escherichia coli diarrhea of travelers: a prospective study of american peace corps volunteers. | travelers' diarrhea was studied prospectively in a group of 39 american peace corps volunteers (pcvs) during their first five weeks in kenya. twenty-seven developed diarrheal disease and 12 remained well. multiple episodes were documented in 11 of the symptomatic volunteers. enterotoxigenic escherichia coli of many serotypes producing heat-labile and/or heat-stable enterotoxin were isolated from 17 of the 27 volunteers with diarrhea and from 1 of the 12 well volunteers. the enterotoxigenic e. co ... | 1977 | 196131 |
| the action of disinfectants on lamb rotavirus. | 1977 | 197685 | |
| antigenic relationship between human and bovine rotaviruses as determined by neutralization, immune adherence hemagglutination, and complement fixation tests. | guinea pig antiserum to bovine rotavirus does not neutralize human rotavirus. bovine and human rotaviruses were, however, extensively cross-reactive when examined by complement fixation and immune adherence hemagglutination tests with antiserum to either virus. the immune adherence hemagglutination test was 16- to 32-fold more sensitive than the complement fixation test in detecting rotavirus. | 1977 | 198371 |
| pathological changes in the small intestine of neonatal pigs infected with a pig reovirus-like agent (rotavirus). | 1977 | 198438 | |
| further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses. | polyacrylamide gel electrophoretic analysis of purified preparation of the simian rotavirus sa-11 indicated eight polypeptide components that migrated in a manner remarkably similar to those of the previously characterized human and calf rotaviruses. analyses of preparations of single-shelled and double-shelled particles of human, calf, and simian an rotaviruses have also permitted assignment of the polypeptides to the inner or outer shells. the major components of the outer shells of each virus ... | 1977 | 198589 |
| human rotavirus and intussusception. | 1977 | 198658 | |
| [application of enzyme-linked immunosorbent assay (elisa) to the detection of calf rotavirus and rotavirus antibodies (author's transl)]. | 1977 | 199102 | |
| [rotavirus in children with severe malnutrition]. | the study comprised 19 children with severe protein-calorie malnutrition admitted to hospital nacional de niños. with one exception, all of the patients complained of diarrhea at the examination. samples of stools were collected for bacteriologic, parasitologic and virologic studies. rotavirus was shown in 4 cases and adenovirus in another 4. enteropathogenic escherichia coli, shigella and salmonella were isolated in 9 different children. it was possible to demonstrate at least one potentially p ... | 1977 | 199211 |
| rotavirus gastroenteritis. | 1977 | 199315 | |
| virions associated with acute gastroenteritis in vancouver, 1976. | virions were identified by electron microscopic observation of unconcentrated extracts of feces obtained from 55 (33%) of 167 children aged 3 weeks to 5 years who were hospitalized with acute gastroenteritis at children's hospital, vancouver between january and december 1976. morphologic types included 30 rotavirus, 15 adenovirus, 5 astrovirus and 5 picornavirus-like particles. | 1977 | 199339 |
| review of enterovirus studies in east africa. | 1977 | 199414 | |
| filter paper solid-phase radioimmunoassay for human rotavirus surface immunoglobulins. | a filter paper solid-phase radioimmunoassay has been developed. filter paper disks adsorbed a large amount of rotavirus and serum globulin and gave small mean variation of coating and low background binding. the rotavirus isolated from stools from infants with acute enteritis 1, 3, and 4 days after onset of symptoms was shown to be already covered with immunoglobulin g (igg), iga, and igm antibodies by this radioimmunoassay, by immunoelectrophoresis, and by immune electron microscopy. the immuno ... | 1977 | 199613 |
| human rotavirus enteritis induced in conventional piglets. intestinal structure and transport. | to better understand the pathogenesis of infantile viral gastroenteritis, we studied na+ and cl- fluxes in vitro in short-circuited jejunal epithelium from 8-10-day-old piglets after infection with a standard dose of human rotavirus given via nasogastric tube. 11 infected piglets, all of whom became ill, were compared with 9 uninfected, healthy litter-mates. when killed 72 h after infection, intestinal villi were shorter and crypts deeper (p less than 0.025) in duodenum, upper jejunum, and mid-s ... | 1977 | 199622 |
| separation and infectivity of two particle types of human rotavirus. | two morphological types of human rotavirus particles were separated in caesium chloride density gradients. the particles of higher density ('incomplete' particles without an outer capsid layer) banded at a density of 1-38 g/ml, while the less dense ('complete' particles with an outer as well as an inner capsid layer) banded at a density of 1-36 g/ml. some particles were found with an incomplete outer layer of capsomeres. the particle/infectivity ratio for tissue cultures of the fraction containi ... | 1977 | 199702 |
| enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. | an enzyme-linked immunosorbent assay for the diagnosis of rotavirus infection in calves is described. the assay was more efficient for the detection of rotavirus antigens in calf feces than were electron microscopy and immunoelectroosmorphoresis. | 1977 | 200635 |
| distribution and titres of rotavirus antibodies in different age groups. | three hundred and fifty-seven sera selected at random from hospital patients of all ages were examined for rotavirus antibodies using indirect immunofluorescence (fa) and complement fixation levels (cft). three hundred and fourteen of these were also tested for neutralizing antibodies to human rotavirus. sera from patients admitted with a diagnosis of acute gastroenteritis were excluded from this survey. fa antibodies were found in newborn infants but fell to undetectable titres at 3 months. the ... | 1977 | 200676 |
| cell culture propagation of porcine rotavirus (reovirus-like agent). | two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ... | 1977 | 201198 |
| diarrhea in calves: diagnosis and incidence in the north central states. | 1977 | 220632 | |
| review of rotavirus infections of man and animals. | 1977 | 220639 | |
| rotaviruses in venezuelan children with gastroenteritis. | rotaviruses were identified by electron microscopy in 19 of 26 (73%) venezuelan children with gastroenteritis. noncultivable adenoviruses and small (29-30 nm), spherical viruses were also observed in a few cases. bacterial pathogens were isolated from five children (19%), all of whom were also carrying rotaviruses. | 1977 | 842776 |
| biochemical studies on a reovirus-like agent (rotovirus) from lambs. | ten polypeptides were detected in double-capsid lamb rotavirus; four of these appeared to be associated with the outer capsid. lamb rotavirus rna, which consisted of 11 or 12 segments, differed from pig rotavirus rna in the electrophoretic mibility of one of the genome segments. | 1977 | 850308 |
| rotavirus infections in calves. ii. experimental infections with a dutch isolate. | rotaviruses were observed electron microscopically in clarified faecal samples from calves with diarrhoea. several herds with a history of scouring among neonatal calves were shown to be infected with rotaviruses. a bacteria-free faecal filtrate from a rotavirus positive sample administered orally induced diarrhoea in one out of two colostrum-deprived calves. both calves excreted rotaviruses in their faeces and serum antibody responses were demonstrated by complement-fixation and indirect immuno ... | 1977 | 857337 |
| propagation of the rotavirus of neonatal calf diarrhea in fetal intestinal cell cultures. | bovine fetal intestinal cells were successfully propagated in monolayer cultures for up to 21 passages. infection of these cells with the rotavirus of neonatal calf diarrhea resulted in a cytopathic effect that was more obvious than in infected bovine fetal kidney cells. | 1977 | 861843 |
| a survey of rotaviruses in sheep in scotland. | 1977 | 867760 | |
| clinical, laboratory, and epidemiologic features of a viral gastroenteritis in infants and children. | we studied 27 infants admitted to the hospital with acute diarrhea caused by human rotavirus (hrv) and obtained additional data on fecal excretion from ten outpatients with the same infection. the disease was characterized by watery diarrhea with fever and vomiting at the onset, isotonic dehydration, compensated metabolic acidosis, and increased concentrations of sodium and chloride but low concentrations of sugar in stools. diarrhea usually ceased in three to four days when oral feedings were r ... | 1977 | 887336 |
| [seroepidemiological investigations on the epidemiology of human rotavirus infections (author's transl)]. | 263 sera of infants and children up to 10 years of age were tested for antibodies (cf-test) against nebraska calf diarrhoea virus (ncdv) which is antigenically related to the rotavirus of man; in addition the mean antibody titers in different age groups were investigated. antibodies of maternal origin were eliminated during the first year of life. most of the infections occurred during the second year of life and at the end of this year 40-50% of the investigated children demonstrated antibodies ... | 1977 | 899370 |
| [infectious agents in diarrhea of hospitalized children in costa rica]. | shigella, toxigenic escherichia coli (stable toxin) and rotaviruses were frequently found among 50 children hospitalized with diarrhea studied during one year. these agents were less common among 45 controls without diarrhea, of comparable age and from the same wards as the cases reported. there was a greater frequency of respiratory symptoms in the diarrhea associated with rotaviruses. these were characterized by frequent bowel movements and vomiting and often fever. the bacterial diarrheas sho ... | 1977 | 911458 |
| [diarrhea outbreak and bacterial resistance to ampicillin in newborns]. | twenty-eight healthy neonates from the san juan de dios hospital were studied to determine the pattern of antibiotic resistance of indigenous intestinal bacteria. sixty-eight per cent of infants had enterobacteriaceae resistant to several wide-spectrum antibiotics, including ampicillin; 28 per cent of the cultures had plasmid-mediated ampicillin resistance. in the course of the study, an outbreak of 10 acute cases of diarrhea occurred, not associated to any of the commonly known agents, includin ... | 1977 | 242927 |
| a microbiological investigation of acute summer gastroenteritis in black south african infants. | a microbiological investigation of black infants suffering from severe acute summer gastroenteritis revealed enteropathogenic agents in 30 out of 37 patients (81%). enterotoxigenic bacteria were isolated from 15 patients (41%). a total of 16 enterotoxigenic strains were isolated, comprising 9 enterotoxigenic escherichia coli strains secreting labile and stable toxin on their own and in combination, and labile-toxin secreting strains of klebsiella pneumoniae (4), enterobacter cloacae (2) and prot ... | 1977 | 325127 |
| gastroenteritis in children: a two-year review in manitoba. i. etiology. | during two years, 1,217 children hospitalized with gastroenteritis at the children's centre in winnipeg, manitoba, canada were studied. bacterial pathogens were present in 25% of these children: enteropathogenic escherichia coli in 120, shigella in 139, salmonella in 24, and multiple pathogens in 18. rotavirus was detected in 54 (11%) of 472 patients examined. rotavirus and enteropathogenic e. coli were the most common pathogens in infants, and shigella was the most common in older children. bac ... | 1977 | 330769 |
| a microbiological study of gastro-enteritis in black infants. | a survey was carried out to determine the aetiological agents responsible for acute gastro-enteritis in black infants. bacteriological examination of 191 patients and 178 age-matched controls showed that salmonellae and shigellae together accounted for only 15% of cases and were harboured by almost 7% of controls. seropositive 'enteropathogenic' escherichia coli was an important cause of diarrhoea, especially in the summer months. enterotoxin-producing e. coli was not a significant cause of gast ... | 1977 | 331509 |
| reovirus-like agent in acute epidemic gastroenteritis in japanese infants: fecal shedding and serologic response. | the reovirus-like agent, sometimes referred to as duovirus or rotavirus, was visualized by electron microscopy in stool extracts from japanese infants and young children with acute epidemic gastroenteritis. the virus particles measured 70 nm in diameter and had double-shelled capsids. one hundred ten (89%) of 124 patients with the gastroenteritis had such virus particles in stools obtained during the acute phase. the virus particles were excreted in the stools usually during the first eight days ... | 1977 | 402427 |
| diagnosis of rotavirus infection by cell culture. | rotaviruses were detected by electronmicroscopy in 35 of 84 specimens of faeces from infants with diarrhoea, and in 31 by fluorescent staining of tissue cultures infected with help of centrifugation. llc-mk2 cells were found to be the most sensitive, although primary and secondary human-embryo-kidney and primary calf-kidney cells could also be used. a micromodification of the tissue-culture method provides a relatively simple technique for the diagnosis of rotavirus infection, for the titration ... | 1977 | 402472 |
| complement-fixing antibody response to rotavirus infection. | a human rotavirus complement-fixing (cf) antigen, prepared by purification of large volumes of fluid feces collected from children with winter diarrhea, was used to study the development and persistence of antibody in children with diarrhea and the prevalence of rotavirus antibody in melbourne. in children with diarrhea, antibody rises were detectable within 4 to 6 weeks of the onset of illness, and the titers usually remained elevated for the next 1 to 2 years. cf antibody did not develop in tw ... | 1977 | 403196 |
| stool viruses in babies in glasgow. i. hospital admissions with diarrhoea. | stools from 183 babies under 2 years of age admitted to ruchill hospital with diarrhoea were examined by electron microscopy, virus culture, bacterial culture and light microscopy. as far as possible, several stools were examined from each patient and the results showed rotaviruses, astroviruses and other viruses in association with symptoms, as well as the expected bacterial pathogens. examination of several stools from the same patient also showed that in this age group the viral flora of the ... | 1977 | 403233 |
| rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis. | eighty-seven faecal samples from infants and children suffering from acute gastroenteritis were investigated for the presence of rotavirus by immunoelectroosmophoresis (ieop) and electron microscopy (em). sixty-one % of the samples contained rotavirus antigens when examined by ieop whereas only 50% were diagnosed as positive em. however, where it was possible to perform em within the same day that the sample was received it took 24 h to establish the diagnosis by ieop. the high sensitivity of th ... | 1977 | 406356 |
| aetiology of acute gastroenteritis in infancy and early childhood in southern india. | the aetiology of acute gastroenteritis was studied in 50 infants and young children. bacterial pathogens were isolated in 33, enteropathogenic e. coli (epec), salmonella, and shigella being the commonest isolates. rotaviruses were detected in the stools of 13 of the cases. all children with gastroenteritis in whom rotavirus was detected were seen during the months july to december. in 30 children who served as controls, epec were isolated in 6, but rotavirus was detected in none. it is concluded ... | 1977 | 406857 |
| demonstration of size variation of rna segments between different isolates of calf rotavirus. | polyacrylamide gel electrophosesis of rna extracted from wild rotavirus isolates and cell culture adapted virus revealed a significant variation in the molecular weight of individual rna segments. the major differences were observed between wild isolates on the one hand and the adapted strain on the other hand. the slight variations that were observed between different wild isolates were found regularly and appeared to be related to the origin of the samples. | 1977 | 407327 |
| [rotavirus and gastroenteritis in the newborn infant. initial observations]. | a simplified method for the diagnosis of rotavirus by electron microscopy is presented. the incidence of these viruses is observed in new-born infants and the relationship with acute gastroenteritis is recalled. | 1977 | 408044 |
| solid-phase radioimmunoassay for the detection of rotavirus. | a solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. the parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. compared with electron microscopy, the assay is up to 10 times as sensitive for detection of the virus in stool and up to 128 times as sensitive for detection of a purified virus antigen. in a field study on stool specimens it was at least as effici ... | 1977 | 863510 |
| breast-feeding: main promoter of infant health. | unique immunological factors in breast milk are responsible for the protection it affords infants against pathogenic agents. this protection is particularly effective against the infectious agents which invade or colonize the small and large intestine. factors interfering with enterotoxigenic and enteroinvasive bacteria, and with entero- and rotaviruses, have already been detected in colostrum and mature human milk. moreover, the biochemical composition of human milk makes it the best infant foo ... | 1978 | 362883 |
| the rate of development of postmortem artefact in the small intestine of neonatal calves. | the rate of development of postmortem artefacts was investigated in the mucosa of the small intestine of a calf dually infected with an enteropathogenic strain of esch. coli and rotavirus, and an uninfected control calf. samples were removed under general anaesthesia from the proximal, middle and distal small intestine and compared with those taken from adjacent sites 1-30 min after severing the major blood vessels of the neck. in the challenged calf, changes occurred in the villous mucosa by 3 ... | 1978 | 656317 |
| viruses and diarrhea--a review. | a critical analysis of the literature shows that only a few viral agents have been proved by accepted criteria to cause acute gastroenteritis. rotavirus, of the rna family reoviridae, is the cause of most childhood gastroenteritis. the parvovirus-like agents (norwalk, hawaii, mc, and w) are proved agents of epidemic gastroenteritis. there is good evidence that the marcy, fs, and niigata agents were the causes of gastroenteritis outbreaks. thirty percent of all acute gastroenteritis episodes in l ... | 1978 | 343580 |
| a prospective study of enteropathogenic escherichia coli in endemic diarrheal disease. | the rate of isolation of escherichia coli belonging to the traditional serotypes enteropathogenic for infants was studied prospectively in two groups. group 1 consisted of children with diarrhea and of controls without gastrointestinal disease who were matched for age and inpatient or outpatient status. group 2 consisted of families entered in a prospective study of rotavirus infections. in group 1 enteropathogenic escherichia coli were found in 13 (6%) of 220 children younger than 12 months of ... | 1978 | 344810 |
| interventions in diarrheas of infants and young children. | to prevent diarrhea, efforts must be directed toward decreasing environmental contamination of enteropathogens, altering exposure of persons to naturally occurring agents, or to increasing host tolerance to prevalent enteropathogens. a vaccine is feasible in the control of certain enteric infections among high risk groups: typhoid fever and cholera, in areas of hyperendemicity or during an epidemic, shigellosis in institutionalized populations that are at risk to develop illness due to known pre ... | 1978 | 359524 |
| simplified radioimmunoassay for detection of human rotavirus in stools. | a simplified radioimmunoassay (ria) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. this microtiter solid-phase ria utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. one hundred thirteen pediatric stool samples were tested. thiry-five specimens known to be positive for rotavirus by electron microscopy were also positive by ria. all ... | 1978 | 216754 |
| [detection of an agent similar to rotavirus in infantile gastroenteritis]. | the etiology of nonbacterial enterovirus infections in children was studied in autumn and winter under conditions of focal outbreaks in moscow hospitals. electron microscopy revealed a reovirus-like agent in concentrated suspensions of feces collected in the acute period of the disease from children suffering from diarrhea. examinations of paired sera from the patients by the cf test with the antigen of nebraska calf disease virus established significant rises in antibody titers in 68.3% of case ... | 1978 | 216151 |
| rotavirus infections in families. a clinical and virological study. | among 25 family members of 8 children with acute rotavirus gastroenteritis (8 siblings and 17 adults) gastroenteritis was recorded in 9 (5 siblings and 4 adults), and minor symptoms in 8 (2 siblings and 6 adults). a rotavirus infection was diagnosed in 7 of the family members with gastroenteritis and was probably the cause of the disease in the remaining 2 in this group. four of the 8 family members with minor symptoms were infected with rotavirus, whereas no infection was detected among the 8 f ... | 1978 | 214850 |
| the detection of rotavirus specific antibody in colostrum and milk by elisa. | the blocking method of elisa for the detection and titration of rotavirus-specific antibody in colostrum is described. the results obtained were positively correlated with those of a neutralizing antibody test. on one farm colostrum samples were obtained over a period of 18 months. no relationship was found between the titer of colostrum obtained shortly after calving, and the development of rotavirus-associated diarrhoea in calves. on a second farm only samples obtained during the calving seaso ... | 1978 | 218492 |
| the influence of colostrum on neonatal rotaviral infections. | 1978 | 218491 | |
| the effect of vaccination on titres of antibody to rotavirus in colostrum and milk. | the continued presence of antibody to rotavirus in the gut of the neonatal ruminant has been shown to be important in protecting against clinical disease associated with rotavirus infections. this effect is dependant upon the amount of antibody included in the diet. titres of antibody to rotavirus may be relatively high in ruminant mammary secretions immediately after parturition but decline rapidly over the course of the first day. the influence of vaccination on the titres of antibody, the con ... | 1978 | 218490 |
| comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces. | immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. the enzyme-linked immunosorbent a ... | 1978 | 214679 |
| the experimental production of diarrhoea in colostrum deprived axenic and gnotoxenic calves with enteropathogenic escherichia coli, rotavirus, coronavirus and in a combined infection of rotavirus and e. coli. | we attempted to produce diarrhoea experimentally in the newborn calf by orally injecting 17 colostrum-deprived calves with two serotypes of escherichia coli ent+ k99+, a rotavirus and a coronavirus. with e. coli alone, a dose of 2 x 10(8) bacteria administered 24 hours after birth causes a mild attack of diarrhoea, whereas 1 x 10(10) bacteria leads to dehydration and death. an inoculation of rotavirus is followed by diarrhoea which always contains large quantities of rotavirus. these animals wer ... | 1978 | 219751 |
| scanning electron microscopy of abomasium and intestine of gnotoxenic calves infected either with rotavirus, coronarivus or enteropathogenic escherichia coli or with rotavirus and e. coli. | neonatal calf diarrhoea induced with several agents of infection was studied by scanning electron microscopy. in a gnotoxenic calf infected with e. coli k99+ ent+, slight lesions of the small intestine were observed and desquamation or puffiness of microvilli occurred. in rotavirus-infected calves, the abomasum was covered with abudant mucous film and appeared to be desquamated. in the small intestine, no desquamation of epithelium was observed. inoculation of the rotavirus and e. coli induced s ... | 1978 | 219752 |
| agents affecting health of mother and child in a rural area of kenya. xi. antibodies against rotavirus in sera from children living in the machakos district of kenya. | 207 sera from children under 5 years of age and living in the machakos district in kenya were tested in the complement fixation test for antibodies against rotavirus. two different antigens were used. the superiority of 'o' antigen over nebraska calf diarrhoea virus antigen was confirmed. after a fall to 29 percent in the 6-8 months age group, the percentage of children with antibodies quickly rises with age to 80 percent at the age of 24 months and to practically 100 percent at the age of 30 mo ... | 1978 | 219569 |
| production of calf rotavirus hemagglutinin inhibitors in the infected cell culture fluid. | 1978 | 216892 | |
| [corona- and rotavirus enteritis in newborn calves in bulgaria]. | in the course of investigations into enteritis enzootics in newly-born calves, 55 fecal samples have been studied through direct electron microscopy by the negative contrast method. rota viruses were found in eight of them, corona viruses in 38.2 per cent of the cases, and both rota- and corona viruses in 49.1 per cent of the cases. two types of rota-viral particles were observed: with and without supercapsids. also two types of corona-viral particles were established. the first type was relativ ... | 1978 | 223276 |
| rotavirus gastroenteritis in hospitalized infants and young children in mexico city. | 1978 | 228369 | |
| elisa: enzyme-linked immunosorbent assay. | similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. the end point is a color change that can be assessed by colorimetry or with the naked eye. various techniques of elisa are described, along with examples of current and potential clinical applications. | 1978 | 215505 |
| prevalence of antibody to the norwalk agent by a newly developed immune adherence hemagglutination assay. | an immune adherence hemagglutination assay (iaha) for the detection of antibody to the norwalk agent of acute epidemic nonbacterial gastroenteritis was developed using as antigen virus purified from stool from an experimentally infected volunteer. the assay was sensitive and specific and was efficient for detecting norwalk antibody seroresponses. the prevalence of norwalk antibody in various groups in the united states was studied. antibody to the norwalk agent was acquired gradually, beginning ... | 1978 | 215713 |
| enteropathogenic organisms. new etiologic agents and concepts of disease. | 1978 | 215856 | |
| machakos project studies: agents affecting health of mother and child in a rural area of kenya. vii. the incidence of diarrhoeal disease in the under-five population. | the outcome of three years of fortnightly diarrhoea surveillance of about 4000 children under the age of five is described. the two-weekly incidence of diarrhoea in children in the age group 0-5 months, 6-11 months and 12-23 months, who were reported and/or observed to be ill, was 3.4%, 5.6% and 3.4% respectively. a decline in the attack rates of diarrhoea not associated with measles at the end of 1975 and the beginning of 1976 corresponds with a similar decline in the incidence of measles and m ... | 1978 | 216135 |
| [rotavirus gastroenteritides in infants and toddlers]. | 1978 | 216499 | |
| prevalence of rotavirus antibody in infants and children. | 1978 | 217819 | |
| [rota virus infantile gastroenteritis: clinical and epidemiological study]. | 1978 | 218164 | |
| hemagglutination and hemagglutination-inhibition studies with a strain of nebraska calf diarrhea virus (bovine rotavirus). | a hemagglutinin has been prepared from nebraska calf diarrhea virus (ncdv) propagated in bs-c-1 cell line. after cesium chloride centrifugation, the hemagglutinin of the bovine rotavirus was found to be associated with intact virions (density 1.355 g/ml) but not with virions lacking an outer capsid layer (density 1.375 g/ml). in hemagglutination-inhibition (hai) tests, the hemagglutinin reacted specifically with ncdv serum, and hai seroconversions were detected in some sera tested. cross-reactio ... | 1978 | 201585 |
| [epidemiological aspects of gastroenteritis dut to rotavirus]. | during december 1976 to january-march 1977, 13/53 children less than 5 years of age were admitted with acute enteritis caused by rotaviruses to the hospital del niño, dif. there is already convincing evidence that this new virus will prove to be the most important etiological agent of sporadic acute enteritis in young children. | 1978 | 202294 |
| a simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters. | organic compounds in cell culture harvests known as membrane-coating components (mcc) prevent virus adsorption to membrane filters. blending cell culture harvests with fluorocarbon removed the mcc and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. subsequent elution with high ph buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold. | 1978 | 202572 |
| the role of breast-feeding in the prevention of rotavirus infection. | breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. an experimental murine-rotavirus model was established by infecting the second litters of dams 4 weeks after infecting their first litters. antibodies were absent from human and murine colostrum and milk specimens despite being present in virtually all sera, ... | 1978 | 202710 |
| location of type-specific antigens in calf rotaviruses. | rotavirus antisera with and without neutralizing activity to calf rotavirus were compared for their ability to agglutinate calf rotavirus particles with and without an outer capsid layer. particles without the outer capsid layer were agglutinated by antisera with immunofluorescent antibody activity and by antisera with immunofluorescent antibody and neutralizing activity. particles with the outer capsid layer were agglutinated only by antisera with neutralizing activity. the neutralization test ... | 1978 | 84819 |
| search by immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii in crohn's disease. | crohn's-disease tissue was tested by indirect immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii. no reactions were obtained with anti-serum to p. maltophilia and m. kansasii, and a granular fluorescence seen with rotavirus antibody was probably non-specific. | 1978 | 80631 |
| rotavirus in travelers' diarrhea: study of an adult student population in mexico. | the role of rotavirus in adult diarrhea was evaluated in 165 students attending a mexican university. students were divided into three groups: newly arrived summer students from the united sttes, regular students from the united states, and mexican and venezuelan students. ninety-one students with diarrhea and 74 corresponding, matched, asymptomatic control students were included in the study. the frequency of rotavirus in stools was determined by electron microscopy with use of the pseudoreplic ... | 1978 | 204710 |
| prevalence of antibodies to a rotavirus in black and white populations in south africa. | a serological survey of antibodies to a rotavirus in 252 sera from black and white south africans was performed by means of an immunofluorescence test using cells infected with simian rotavirus, sa 11, as antigen. the results indicate widespread presence of this agent in south africa and also that the black population is exposed to a greater extent than the white population. | 1978 | 206980 |
| [pediatrics in 1977]. | 1978 | 206954 | |
| rotavirus as a cause of diarrhea in pigs. | a rotavirus (reovirus-like agent) was associated with diarrheal diseases occurring in 1- to 4-week-old suckling pigs in 8 herds and in weaned pigs in 2 herds. transmissible gastroenteritis virus was also detected in 2 of these herds, as was enteropathogenic escherichia coli in 5 herds. morbidity was generally greater than 80% in pigs of the affected age group within these herds, and mortality from diarrhea ranged from 7 to 20%. the disease due to rotavirus in suckling pigs appeared similar to th ... | 1978 | 203565 |
| [virus diarrhea in calves in south africa]. | the importance of diarrhoea in calves is briefly discussed. the role of viruses as primary invaders of the intestinal epithelium is stressed. the pathogenesis and problems encountered in diagnosing virus associated diarrhoea as well as the morphology of rota and coranavirus are discussed. possible methods of prevention and treatment are briefly mentioned. | 1978 | 212561 |
| epidemiology of human rotavirus types 1 and 2 as studied by enzyme-linked immunosorbent assay. | to determine the relative importance of two known serotypes of human rotavirus, we developed an enzyme-linked immunosorbent assay to differentiate serotype-specific rotavirus antigen and antibody. using this technic, we studied the epidemiology of the two serotypes in acute gastroenteritis. seventy-seven per cent of 414 rotavirus isolates were type 2, and the remainder were type 1. the serotype distribution was similar in specimens from children in washington, d.c., and other parts of the world. ... | 1978 | 212678 |
| rotavirus diarrhoea of foals. | 1978 | 213048 | |
| concurrent rotavirus and salmonella infections in foals. | 1978 | 213413 | |
| a long-term survey of rotavirus infection in japanese children with acute gastroenteritis. | human rotavirus was detected by electron microscopic examination of the stools of 320 (63%) of 506 infants and young children hospitalized with acute gastroenteritis between december 1974 and march 1977. serologic responses to infection with the rotavirus were revealed by the complement-fixation test in 130 (70%) of 185 patients examined. during the study period three epidemics of human rotavirus infection occurred during the winter months. the peak incidences occurred in january 1975 (88% of pa ... | 1978 | 213501 |
| an investigation into the possible role of the family unit in the transmission of rotavirus infections of children. | 1978 | 213802 | |
| rotavirus infection in avian species. | 1978 | 213873 | |
| [coronavirus and "astrovirus" observed in stools of children with gastroenteritis (author's transl)]. | in a year's survey (december 1976-november 1977) of 190 children admitted to hospital with acute gastroenteritis, an "aetiological" agent was found in stools by electron microscopy in 41% of cases. rotavirus were found in 34% of patients. we also found coronavirus-like particles and astrovirus-like particles in 25 and 5 stools, respectively. improvements are awaited to demonstrate the responsibility of these virus in gastroenteritis. | 1978 | 214002 |
| acute infantile gastroenteritis caused by rotavirus in japan. | we had an outbreak of acute infantile gastroenteritis accompanied by milky-white stool (called hakuri in japanese) during the winters of 1976 and 1977. stool specimens collected from 72 cases of hakuri were studied by negative-staining electron microscopy. rotavirus was detected with a very high frequency (89%). rotavirus obtained from one of the patients was isolated and passaged in cultures of primary human embryonic kidney cells. viral antigens could be detected in the cytoplasm of the cells ... | 1978 | 214305 |
| role of rotavirus (reo-like) in weanling diarrhea of pigs. | piglets weaned abruptly and precociously at 3 weeks of age and placed in a crowded nursery commenced diarrhea 3 to 5 days later. death losses were low (approximately 6%), but weight gain ceased for 2 weeks. large numbers of rotavirus (reo-like) particles were seen by electron microscopy in diarrhetic fluids. sections of intestines showed a loss of adsorptive surface in that villi were shortened and fused with adjacent villi. immunofluorescence revealed rotaviral antigens within damaged enterocyt ... | 1978 | 214458 |
| secretory antibody directed against rotavirus in human milk--measurement by means of enzyme-linked immunosorbent assay. | human milk contains antibodies to a variety of enteropathic agents. we utilized the method of enzyme-linked immunosorbent assay to investigate anti-rotavirus secretory iga in 113 human milk and colostral specimens from a rural area in guatemala, 32 colostral specimens from an urban area of costa rica, and 12 from an urban area of the united states. anti-rotavirus sciga was found in all colostral samples and in 94% of the milk specimans. both the absolute concentration of anti-rotavirus sciga and ... | 1978 | 214534 |
| nosocomial infantile gastroenteritis associated with minirotavirus and calicivirus. | 1978 | 214535 | |
| detection of antibody to rotavirus by counterimmunoelectrophoresis in human serum, colostrum, and milk. | 1978 | 214536 | |
| jejunal microbial flora of southern indian infants in health and with acute gastroenteritis. | the microbial flora of the jejunal lumen of 28 infants with acute gastroenteritis was compared with that of a group of 10 normal infants. the jejunum of control subjects harboured an "oral" type of flora and in a few instances enterobacteria in small numbers. the concentrations of all but one of the groups of organism were higher in the patients than in controls, and the differences were of statistical significance for enterobacteria and lactobacilli. in eight subjects, the same pathogen was ide ... | 1978 | 214565 |
| isolation of a reovirus-like agent (rotavirus) from neonatal calf diarrhea in japan. | 1978 | 214670 | |
| a study on the ultrastructure of human rotavirus. | 1978 | 214956 | |
| viral diarrhea. etiology and control. | 1978 | 215028 | |
| synthesis of coreless, probably defective virus particles in cell cultures infected with rotaviruses. | pk-15 cells infected with pig and lamb rotavirus strains which were not adapted to serial growth in cell cultures were examined by electron microscopy. a major difference between virus morphogenesis in the initial passage in pk-15 cells and in intestinal epithelial cells was the generation of large numbers of coreless virus particles in pk-15 cells. the numbers of coreless particles increased with increasing multiplicity of infection. infectious virus was synthesized in pk-15 cells, but a variab ... | 1978 | 215104 |
| isolation of rotavirus from foals with diarrhoea. | a rotavirus, morphologically similar to other known rotaviruses, was demonstrated in the faeces of 5 foals with diarrhoea on two properties. four of these 5 samples produced specific intracytoplasmic fluorescence in cell culture when reacted with calf rotavirus antiserum conjugate. sixteen affected foals from both properties were depressed, did not suckle and became recumbent. most had a watery diarrhoea which lasted for 3 days and resulted in some dehydration and loss of body condition. sick fo ... | 1978 | 215112 |
| comparison of human rotavirus disease in tropical and temperate settings. | human rotaviruses (hrv) are a common cause of acute nonbacterial gastroenteritis in pediatric patients. a prospective study of hrv disease in a temperate (dallas) and a tropical (san jose, costa rica) setting demonstrated differences in seasonal distribution. in both locales, hrv accounted for 50% to 60% of acute nonbacterial gastroenteritis episodes from december through february; this period corresponded to the cooler months of winter in dallas and to the dry season in san jose. during the res ... | 1978 | 210656 |
| isolation of rotavirus from calves in the federal republic of germany. | 1978 | 207058 | |
| central nervous system involvement in patients with rotavirus gastroenteritis. | two children with rotavirus gastroenteritis are presented. the first case developed a fatal reye's syndrome and the other one encephalitis with slow recovery. the rotavirus diagnosis was made in both cases by electron microscopy and a significant rise in antibody titres to nebraska calf diarrhea virus was seen in one of the two patients. | 1978 | 204984 |
| [rotaviruses as pathogens for calf diarrhea: prevalence of complement fixing antibodies in fattening calves]. | 1978 | 204995 |