TitleAbstractYear(sorted ascending)
effect of tunicamycin on human rotavirus morphogenesis and infectivity. brief the presence of tunicamycin, decreases in the number of double-shelled human rotavirus particles coincided with a reduction in cell lysates infectivity. the majority of particles within the endoplasmic reticulum were membrane bound, "enveloped" particles.19846089709
[virologic and immunologic studies on rotavirus excretion in children]. 19846089974
comparative study of bovine rotavirus isolates by plaque assay.rotaviruses were isolated on bsc-1 cells from counterimmunoelectrophoresis and/or electron microscopy positive intestinal contents from two asymptomatic and six diarrheic calves from quebec. the plaque assay was performed using these lines and agar overlay medium containing trypsin and deae-dextran. this assay was used to compare the quebec isolates to an attenuated american strain (ncdv) and another strain (th) obtained from france. the ncdv strain produced plaques that were significantly large ...19846089982
prevalence of rotavirus and coronavirus antigens in the feces of normal cows.the prevalence of rotavirus and coronavirus shedding by adult cows was investigated using capture enzyme-linked immunosorbent assays. fecal samples from 121 cows in a single herd were tested for the presence of rotavirus and coronavirus, either free or complexed with immunoglobulin. free rotavirus was not detected in any samples while rotavirus-immunoglobulin complexes were detected in 53 of 121 (44%) samples tested. in contrast, free coronavirus was detected in six (5%) samples and coronavirus- ...19846089985
the radiographic findings in severe rotavirus-induced colitis.rotavirus infections are a common cause of childhood gastroenteritis but are rarely severe enough to justify radiographs. however, there appears to be a small subset of children in whom a rotavirus-induced diarrheal illness accompanied by bloody stools is sufficiently severe and protracted to warrant barium studies. we have observed spasm and minute mucosal ulcerations of the colon in three of these children. these findings, under other circumstances, would have prompted the diagnosis of ulcerat ...19846090466
acute diarrhoea and rotavirus infections in young children in kuwait.the role of rotavirus infections in acute diarrhoea in young children was studied over a period of one year. rotavirus was detected by electron microscopy and enzyme immunoassay methods in 40.2% of faecal specimens from 343 children with acute diarrhoea and in 4.7% of 86 controls. the infections were most common in children aged 2-12 months (42.3%). twelve per cent of the rotavirus infected children were also infected with bacterial enteropathogens (salmonellae, shigellae and enteropathogenic es ...19846083746
aetiology of diarrhoea in adequately nourished young african children in durban, south africa.two inter-related hospital-based studies on the causes of diarrhoea in african children who were not overtly malnourished, were performed over a period of two years in durban, south africa. the first study involved 126 inpatients selected for previously untreated diarrhoea of less than 96 h duration. on examination and culture of the stools of these children bacteria were identified in 60%, rotavirus in 20% and parasites in 4%. no pathogens were identified in 33%. the bacteria most frequently en ...19846084467
antigenic and pathogenic relationships of three bovine rotaviruses and a porcine rotavirus.the antigenic relationships between three bovine rotaviruses, uk, cp-1 and pp-1, and a porcine rotavirus were investigated; their pathogenicity for piglets was also assessed. after propagation and cloning in cell culture, the four viruses were used to produce convalescent and hyperimmune antisera in gnotobiotic animals. for the uk and pp-1 viruses, reciprocal virus neutralization tests gave ratios of homologous to heterologous titres in the range 57 to greater than 2300. taking a 20-fold differe ...19846086808
oligosaccharide composition of calf rotavirus.the oligosaccharides of the structural glycoprotein (vp7) of calf rotavirus were characterized. the precursor of vp7 produced in infected cells in the presence of tunicamycin migrated on sds-polyacrylamide gels with an apparent molecular weight 6000 less than the glycosylated glycoprotein. endoglycosidase (endo) h digestion of the mature virus resulted in a decrease of 5000 in the molecular weight of vp7 in two discrete stages. analysis of endo h-treated, 3h-labelled digestion products of vp7 on ...19846086809
propagation of rotaviruses in the presence of chicken serum.the ability of mammalian rotaviruses to replicate in bsc-1 and cv-1 cell cultures was facilitated by the presence of 4% chicken serum. viral plaques tended to be larger and appear more quickly than in cultures without added chicken serum. it is proposed that chicken serum facilitates plaquing of rotavirus due to its lack of trypsin inhibitors.19846086811
comparison of bovine, simian and human rotavirus structural glycoproteins.the structural glycoproteins of calf (bdv 486), human (wa) and simian (sa11) rotaviruses were compared for sensitivity to endoglycosidase (endo) h. the calf and human virus glycoproteins were reduced by approximately 5000 molecular weight in two equal stages. the simian rotavirus glycoprotein sustained only one 2500 molecular weight decrease. the effect of the host cell on the viral oligosaccharide composition was examined. endo h digests of calf rotavirus 82-124 extracted from stool and its pro ...19846086812
identification of a bovine rotavirus gene and gene product influencing cellular attachment.preliminary characterization of naturally occurring temperature-sensitive bovine rotavirus mutants, generated via gene reassortment, indicated that the product of gene segment 10 can influence virus adsorption to ma-104 cells. cell-free translation of this gene segment in a rabbit reticulocyte system was then utilized to identify a polypeptide with a molecular weight of ca. 21,500 as the primary translation product. this identification provides a basis for further studies of rotavirus assembly a ...19846086958
pseudoreplica electron microscopy for the detection of rotavirus: comparison with high-speed centrifugation electron microscopy and elisa.three-hundred and sixty-three stool specimens from patients with diarrhoea were examined for rotaviruses to compare the sensitivity of the pseudoreplica technique (psd-em) to that of high-speed centrifugation em (hsc-em) in relation to a commercially available (rotazyme, abbott) enzyme-linked immunosorbent assay (elisa). in elisa-positive cases, both methods were of equal sensitivity. however, in borderline (+/-) and elisa-negative specimens, psd-em detected 31 of 48 (64.6%) and 18 of 229 (8%) p ...19846094611
a cryptogram for recording rotavirus strains: the rotacode.the rna genome of rotaviruses consists of 11 segments in four size-classes which can be separated by polyacrylamide gel electrophoresis, although 11 separate bands are not shown by all strains. we propose a cryptogram (rotacode) based on the relative distance of migration of adjacent bands in each size-class for coding the typical pattern of each strain of virus. this provides a shorthand for recording details of each strain and for grouping electrophoretically similar strains. rotacode was foun ...19846094663
molecular cloning and sequencing of the reovirus (serotype 3) s1 gene which encodes the viral cell attachment protein sigma 1.the complete sequence of the reovirus (serotype 3) s1 gene was obtained by using cloned cdna derived from the rna segment. this gene is 1416 nucleotides in length and contains two open reading frames. the first reading frame has a coding capacity of 455 amino acids, sufficient to account for the known s1 product, protein sigma 1 (42,000 mw). it possesses a signal peptide as well as three possible glycosylation sites. no homology could be detected when this gene sequence and the deduced amino aci ...19846095208
enzyme immunoassay for subgrouping human rotaviruses using monoclonal antibodies.rotavirus group-specific (common in human rotaviruses) and subgroup 1-specific monoclonal antibodies were established and an enzyme immunoassay was developed for subgrouping human rotaviruses.19846095489
milk fat and gastrointestinal illness.the population of a pediatric practice was followed prospectively and determinants of acute gastrointestinal illness were evaluated in a nested age-matched case-control study. children over age one taking low fat milk as their only milk source in the three weeks prior to illness had five times the risk of a doctor's visit for acute gastrointestinal illness as did children taking only whole milk during the same time period. this increased risk could not be explained by numerous potentially confou ...19846095691
[rotavirus excretion in infants. virologic and immunologic aspects]. 19846095723
[detection of rotavirus in the feces of children with diarrhea].bacteriological and virological examinations of feces from sick children diagnosed as having an "enteric infection of obscure etiology" were carried out. out of 30 fecal specimens 6 showed positive results in agar gel diffusion test with antiserum to calf diarrhea rotavirus confirmed by electron microscopy. the relationship of virological and bacteriological findings and age of the patients was established. differences in the clinical manifestations of the disease in virus-positive and virus-neg ...19846097048
synergism in experimental mixed infections of newborn colostrum-deprived calves with bovine rotavirus and enterotoxigenic escherichia coli (etec). 19846097066
vaccination of cows with k 99 and rotavirus antigen: potency of k 99 antigen combined with different adjuvants in stimulating milk antibody secretion. 19846097069
pathogenesis and immunoprophylaxis of bovine rotavirus infection. 19846097086
infectious agents associated with diarrhoea in commercial rabbits: a field study.over a period of 9 months the occurrence of parasites, bacteria and viruses has been examined in diarrhoeic rabbits from 21 commercial rabbitries and related with clinical signs and gross and microscopic lesions. infectious disease agents have been found in 71.5% of the animals. escherichia coli (atec) were found to be attached to the luminal intestinal border of 40% of the rabbits examined. this was associated with moderate to high mortality, caecal oedema, severe swelling of mesenteric lymph n ...19846097152
[experimental infection of fattening swine with rotavirus: study of local immunity using coproantibodies].fattened pigs were experimentally infected with porcine rotavirus (osu strain). viral antigens found in the faeces were analysed over a period of two months (elisa method). we have found no correlation between virus excretion and the clinical manifestation of disease. the local immunity has been analysed in the faeces by the coproantibodies antirotavirus. the different classes of antibody, g, m, a and immune complexes have been determined using the elisa method. the level of iga antibodies was h ...19846097155
a rapid screening assay for detecting individual rna species in field isolates of rotaviruses.a method is described that allows the rapid screening of field isolates of rotavirus for the detection of specific viral rna segments. cloned cdna copies of viral genomic rnas are employed for detection in the assay which makes use of the dot-hybridization technique of thomas (1980). the assay developed was shown to be sufficiently sensitive and specific to allow the genetic composition of virions to be determined over a wide range of concentrations. the feasibility of using the method developed ...19846096390
rapid detection of enteric adenovirus and rotavirus: a simple method using polyacrylamide gel electrophoresis.a simple and rapid procedure for identifying adenovirus and rotavirus in stool extracts has been developed. the technique is based on polyacrylamide gel electrophoresis of the virus nucleic acid, but sample preparation is straightforward and does not entail phenol extraction or the use of a radioactive label. furthermore, processing is not influenced by the amount of specimen obtained and is thus suitable for application as a batch testing method. this approach removes the need for specific anti ...19846096404
antigenic variation in latin american human pararotaviruses (a typical rotaviruses) 19846096406
viral diarrhoeas. 19846096438
clinical features and laboratory diagnosis of rotavirus-associated gastroenteritis in infants and children.this study was undertaken to compare countercurrent immunoelectrophoresis (cie) and enzyme-linked immunosorbent assay (elisa) using rotazyme kit to detect rotavirus antigen in the stools of children with gastroenteritis and to compare and contrast the signs and symptoms of rotavirus antigen-positive with antigen-negative children. over 2 years, 171 infant and children admitted to the pediatric in-patient unit at st. luke's hospital, cleveland, ohio, with gastroenteritis were evaluated. stools ...19846096439
the detection of rotaviruses in products of wastewater treatment.rotaviruses present in products of wastewater treatment were assayed in ma104 cells by indirect immunofluorescence. levels in settled sewage, activated sludge and effluent were greater than 10(3) per litre in march and april but virus was not detected during later months. this pattern correlated with the decline in laboratory reports of human rotavirus infection.19846096445
[epidemiologic and electron microscopic study of viral gastroenteritis in kagawa prefecture, japan, revealed three distinct causative agent groups]. 19846096456
identification of an outer capsid glycoprotein of human rotavirus by concanavalin outer capsid glycoprotein of human rotavirus (serotype 1) was localized and characterized by use of lectins, electron microscopy and a modified western blotting technique. lectins with specificity for fucose, galactose, glucose and mannose were mixed with purified single- or double-shelled human rotavirus. aggregates observed by electron microscopy were obtained with double-shelled virus and concanavalin a, the only tested lectin with mannose specificity. by sds-polyacrylamide gel electrophor ...19846096494
faecal and pharyngeal shedding of rotavirus and rotavirus iga in children with 70 children 0-4 years of age with acute diarrhoea, the shedding of rotavirus and the excretion of rotavirus-specific iga antibody in the stools were examined throughout the period of clinical symptoms. quantitative detection of rotavirus and iga was performed by an elisa technique. the maximal rotavirus shedding was found between the second and fifth day and the maximal excretion of iga antibody about the seventh day, which marked the clinical recovery of most children. throat swabs were exam ...19846096502
a serological survey on rotavirus infection in dogs by immune adherence hemagglutination test. 19846096619
canine parvovirus enteritis 3: scanning electron microscopical features of experimental infection.a group of 10-week-old puppies was orally inoculated with canine parvovirus of faecal origin. scanning electron microscopy was used to study and compare the surface topography in both control and inoculated animals. in control dogs the villi were tall and finger-like in shape and numerous irregular transverse circumferential grooves were present on the surface. at higher magnification, the outlines of individual epithelial cells and depressions, interpreted as goblet cells, could be discerned. i ...19846097019
study of genetic reassortment between two human rotaviruses.eight different human rotavirus strains were adapted to replicate in cell culture. in vitro, rotavirus genetic reassortment has been performed between two virus strains, one with a long pattern of segmented genomic rnas and the other with a short pattern in the electrophoretic mobility of genomic rnas. this genetic reassortment was detectable despite the lack of selective markers in the parental strains. moreover, some of the reassorted virus exhibited mixed rna patterns containing extra genomic ...19846097030
[viruses in the feces of patients with viral hepatitis and other enterovirus infections].direct and immune electron microscopy was used to determine the frequency of finding of hepatitis a virus (hav) and other viral agents in feces of patients in relation to the diagnosis and epidemiological situation. hav-containing excretions from patients were analysed ultrastructurally. the highest frequency of hav detection was established in patients in a water-borne and food-borne outbreak of hepatitis a (ha) and was 40.9% and 36.9%, respectively. in patients with ha diagnosis in the period ...19846097043
[immunofluorescent study of the reproduction of human rotavirus in cell culture].the mechanism of rotavirus antigen amplification in cell culture was studied by immunofluorescence. reproduction of human rotavirus in cell culture was shown to begin 6 h after inoculation and to be accompanied by a regular increase in the number of antigen-containing cells. a good correlation with the results of electron microscopic studies was demonstrated. the possibility of reliable detection of rotavirus antigen in cell culture by the fluorescent antibody technique at relatively early inter ...19846097047
an attempt to cultivate human rotavirus in human leukocytes culture (preliminary report). 19846097999
rotavirus in pediatric diarrhoea at siriraj hospital, bangkok, thailand.between june 1982 and may 1983, rotavirus was found in 29% of 248 pediatric patients between 1 month to 5 years of age at siriraj hospital, bangkok, thailand. among 413 household contacts, rv was detected in 9 (2%) of whom 6 developed diarrhoea. rv was not found in age-matched healthy controls. in a serological study, the serological response was 81% in rotavirus diarrhoea cases (17 out of 21) by elisa. by the elisa blocking test, the seroconversion rate was only 29.6% in rotavirus diarrhoea cas ...19846098022
"enveloped" rotavirus particles detected in culture a negative contrast preparation of human rotavirus culture fluid were found membrane-bound "enveloped" virus particles which had been observed only in thin sections of the infected cells. the "enveloped" particles comprised a single-shelled particle and degenerating membrane structure.19846098053
[enteropathogenicity of bovine coronaviruses under field conditions]. 19846098103
studies on the growth of bovine rotavirus in cell cultures. 19846098104
[viral etiology of gastroenteritis]. 19846097282
epidemiology of rotavirus gastroenteritis in infants from the area of bochum as revealed by electrophoresis of genome rna.rotaviruses were detected by enzyme-linked immunosorbent assay (elisa) in stool samples from 504 infants admitted with gastroenteritis to the children's hospital of the university bochum in the period from 1979 to 1984. in most infants rotaviruses persisted in the stools for 6-8 days. rotavirus rna was extracted from stool samples from 203 infants. by acrylamide gel electrophoresis, bands characteristic of rotavirus double-stranded rna were detected in 172 (85%) of these 203 samples in which the ...19846097451
[new developments in rotavirus research]. 19846097517
isolation of a rotavirus from canine diarrheal feces. 19846097734
[acute gastroenteritis and breast feeding in gabon. preliminary results].76 diarrheic infants (from 15 days to 2 years) had a stool examination for rotavirus (elisa method) and a stool culture and were classified according to breast feeding or not. this study was performed in a semi-urban region (haut-ogooué, gabon). the preliminary results suggest a protection by breast feeding against rotavirus and enteropathogenic bacteria, rapid elimination of diarrheic agents may be facilitated by human milk.19846097788
the molecular biology of rotaviruses. vii. detailed structural analysis of gene 10 of bovine rotavirus.cdna cloning and nucleotide sequence analysis have allowed detailed structural studies on rna segment 10 of the u.k. bovine rotavirus to be undertaken. the complete sequence of 751 nucleotides was determined and found to contain only a single long open reading frame capable of coding for a protein of 175 amino acids. the gene has an unusually long 3' untranslated region of 184 nucleotides or some 24.5% of the total sequence, whose start was confirmed by analysing the carboxyterminal amino acid o ...19846099939
isolation and propagation of bovine rotavirus in cell culture. 19846100726
comparison of the polypeptides of bovine rotavirus serotypes. 19846100727
effect of s-adenosylmethionine on human rotavirus rna synthesis.the characteristics of human rotavirus-associated rna polymerase activity have been examined in relation to the effects of ribonucleoside triphosphate analogs and s-adenosylmethionine. these effects were analyzed by testing two forms of activated virus particles: edta- and heat-treated virions. the former lack outer shell proteins, and activation by means of heat treatment does not introduce any apparent modification in virion structure. virus-associated rna polymerase shows similar properties i ...19846090696
infectious diarrhea of infant rats produced by a rotavirus-like agent.during the investigation of an outbreak of diarrhea in suckling rats, a virus morphologically identical to but antigenically distinct from rotaviruses was identified. the disease was characterized clinically by erythema and cracking and bleeding of the perianal skin associated with the excretion of poorly formed fecal pellets, liquid, and gas. light microscopy-observable changes consisted of small intestinal villous atrophy, villous epithelial necrosis, and villous epithelial syncytial cell form ...19846090714
isolation of human rotaviruses with a distinct rna electrophoretic pattern from indonesia. 19846090872
the use of beta-lactamase in enzyme immunoassays for detection of microbial antigens.the sensitivity and performance characteristics of enzyme immunoassays (eia) depend to a great extent on the kinetics of the enzyme-substrate system used as indicator. we labeled a variety of polyclonal and monoclonal immunoglobulins with purified beta-lactamase and used them in sensitive eia systems for the detection of a number of microbial antigens. polyclonal antibodies to rotavirus, adenovirus, and haemophilus influenzae type b polyribitol phosphate and monoclonal antibodies to dengue virus ...19846092474
production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination.nonneutralizing monoclonal antibodies were prepared against two strains, s2 and yo, of human rotaviruses isolated in cell culture. s2-37 and yo-5 antibodies had subgroup i and subgroup ii specificities, respectively. the remaining antibodies (s2-65, yo-71, yo-89, and yo-156) reacted commonly with all the rotaviruses examined. all of the monoclonal antibodies agglutinated exclusively single-shelled particles and immunoprecipitated 42,000-dalton protein, a major component of inner capsid. using th ...19846092528
rotavirus shedding by newborn children.we studied the shedding of rotavirus by newborn children in the nurseries of a large maternity hospital in caracas, venezuela, throughout the year 1982. sixty-two (57%) of 108 children examined shed the virus within the first few days of life. four (6%) of the 62 children who shed rotavirus had diarrhea but only one of them required oral rehydration therapy. the rotavirus specimens were identified as subgroup 2 in an elisa subgrouping assay that employs monoclonal antibodies. analysis of the rna ...19846092529
[comparative studies on the rotavirus syndrome following infection with human rotavirus of the subgroup 1 or 2].human rotavirus infection which heals spontaneously causes gastroenteritis in newborns and infants. 150 pediatric patients infected with rotavirus as diagnosed by elisa suffered from diarrhoea for an average of 3 days, from vomiting for 1 day, and/or fever for 1-2 days. nowadays this disease is known as "human rotavirus syndrome". human rotaviruses can be divided into at least 4 serotype antigens and some 3 further subgroup antigens. the serotype antigens are only detectable biologically (e.g. b ...19846092948
[detection of rotavirus in patients with malabsorption].investigations were carried out to evaluate frequency and pathogenic effects of rotaviruses. for this purpose 53 biopsy specimens were taken from the jejunum, especially of children at an age up to 15. because of their malabsorption the patients have undergone a special gastroenterological treatment for years or they only recently began with it, being suspected of suffering from this disease. rotavirus antigen was indicated in 43% of the cases by means of immunohistology, at which only less part ...19846092960
the commonest rotaviruses from neonatal lamb diarrhoea in england and wales have atypical electropherotypes. 19846093328
cloning of bovine rotavirus (rf strain): nucleotide sequence of the gene coding for the major capsid protein.the genes of the rf strain of bovine rotavirus have been cloned into pbr 322 following the synthesis and hybridization of cdna transcribed from both strands of in vitro polyadenylated genomic rna. cloned rotavirus dnas were assigned to most of the 11 genomic rna segments by northern blot hybridization. the complete sequence of gene 6 that codes for the major inner capsid protein has been determined. the gene is 1356 nucleotides long and possesses an unique long open reading frame that could enco ...19846093360
comparison of a commercial enzyme-linked immunosorbent assay with electron microscopy, fluorescent antibody, and virus isolation for the detection of bovine and porcine rotavirus.the purpose in this study was to compare the sensitivity of a commercial enzyme-linked immunosorbent assay (elisa) with electron microscopy (em), fluorescent antibody (fa), and virus isolation (vi) for the detection of bovine and porcine rotavirus (rv). seventy-three bovine and 116 porcine accessions were evaluated by 1 or all 4 diagnostic tests, where suitable specimens were available. for the bovine samples, agreement was 33% between fa and em, 33% between fa and elisa, and 92% between em and ...19846093642
isolation of murine rotavirus in cell cultures. brief report.murine rotavirus was isolated in primary monkey kidney cells. electrophoretic pattern of genome rna of murine rotavirus was different from that of human rotaviruses. oral administration of cultured murine rotavirus caused mild diarrhea in newborn balb/c mice.19846093743
comparison of three enzyme-linked immunosorbent assays suitable for the detection of antibodies to rotaviruses in epidemiological studies.three enzyme-linked immunosorbent assays which use commercially available reagents are presented as alternatives to the complement fixation procedure for large-scale detection of rotavirus antibodies. comparison of results in 75 sera tested by indirect, competition and blocking elisa and by complement fixation demonstrated that the elisa techniques were rapid, easy to perform and 26 to 29 times more sensitive than complement fixation; furthermore the elisa techniques permitted extrapolation of a ...19846098473
influence of breast milk on nosocomial rotavirus infections in prevent nosocomial rotavirus infections in hospitalized children with various non-gastrointestinal diseases, 30 children (mean age five months) received 200 ml of fresh human milk per day in addition to the normal diet for their age. a matched group of children on formula diet served as a control. fecal samples were routinely screened for rotavirus by a commercial elisa test. in stools containing rotavirus, the virus rna segments were analysed by gel electrophoresis to identify the different ...19846088396
shifts in the electrophoretic pattern on the rna genome of rotaviruses under different electrophoretic conditions.substantial differences in the rna electrophoretic patterns of rotaviruses were observed when the conditions for gel electrophoresis were varied. electropherotypes which seem alike under one set of conditions, may appear significantly different under other conditions such as distinct proportions of acrylamide and bisacrylamide or different running buffer. different patterns were observed for the same rotavirus rna sample even when different voltages were applied to otherwise identical runs; thes ...19846088565
efficiency of human rotavirus propagation in cell culture.this study was designed to find methods to reproducibly propagate human rotaviruses from fecal specimens and to determine the relationship between particle numbers and infectivity. growth of virus was initially compared in primary and continuous lines of monkey kidney cells. primary cells (african green and cynomolgus monkey kidney) supported virus growth directly from fecal specimens much more efficiently than did continuous lines of african green (cv-1) or rhesus (ma104) monkey kidney cells. r ...19846088569
detection of rotavirus in human stools by using monoclonal antibody.a monoclonal antibody, 3f7, that reacts with the common rotavirus antigen on the sixth viral gene product was prepared. it was used in a direct monoclonal antibody radioimmunoassay (ria) as a diagnostic reagent for detection, in 3.5 h, of rotavirus in human pediatric stool specimens. in the 177 samples tested, a concordance of 96% was seen between the monoclonal ria and the well-established and commonly used commercially available rotazyme test. six discrepant specimens that were positive by mon ...19846088573
rotavirus infections in high-risk neonates.during a 12-month period, 260 neonates in a referral neonatal nursery were examined weekly for rotavirus infection. a total of 15.4 per cent were found positive during their stay in the nursery and one-quarter of these neonates had symptoms of diarrhoea. most infected neonates excreted rotavirus in only one stool sample. no seasonal variation was found in the incidence of infections. rotavirus infection was related to low birthweight and length of stay in the neonatal nursery. breast-fed newborn ...19846085100
the major surface glycoprotein of simian rotavirus (sa11) contains distinct epitopes.the polypeptide specificities on monoclonal antibodies previously derived against the sa11 simian, nic bovine, and wa human strains of rotavirus were determined by radioimmunoprecipitation of infected cell lysates. all the monoclonal antibodies derived using nic and wa were found to be directed against the major component of the inner capsid, while most of the sa11 monoclones were directed against the major outer capsid glycoprotein. when several sa11 glycoprotein-specific monoclonal antibodies ...19846085822
[current virus diseases in horses. diseases in foals and respiratory tract infections].at the moment, horse praxis is confronted by two disease complexes which are difficult to fight against as well in prophylaxis as in therapy, but which get an increasing importance. first they concern virus infections of the foals and second primary virus-caused respiratory diseases. foals get infected during the embryonal/fetal development, in the perinatal or postnatal period. normally the infection is caused by latent infected, clinical healthy mares, or in the postnatal period by ubiquitous, ...19846098971
[rotavirus and acute gastroenteritis in children]. 19846098983
[immunofluorescent diagnosis of rota-, corona- and pestivirus infections in calves].bovine hyperimmune fluorescent sera were obtained for indication of the rota- and corona-virus infections in calves, which were highly specific and of immunofluorescent titers within the 1:32-64 range. their testing was carried out via the direct immunofluorescence method, using histologic cross-sectioned material and impression preparations of the mucous membrane of calves died of enteritis as well as cell cultures of fetal calf kidney and organic cultures of calf trachea all contaminated, resp ...19846098987
[human rotavirus persistence].among 250 tissue specimens collected by aspiration biopsy from the duodenum, small intestine, and rectum of 187 patients, human rotavirus was found in 50 cases in marked cryostate sections by means of fluorescent-serological examinations. at a total rate of findings of 26.7%, rotavirus was repeatedly detected in 5.3% in rebiopsies during more than 10 months, therefore active persistence of human rotavirus must be taken into consideration.19846098998
[light and electron microscopic studies of experimental and spontaneous rotavirus infection in calves]. 19846099106
[comparison of diagnostic methods in rotavirus infection: immunofluorescence, elisa and the latex agglutination test].to examine the sensitivity and reliability of a latex agglutination test for detecting rotavirus in faecal samples, we have tested 145 stool specimens from pediatric patients with or without diarrhea, comparing such latex test to immunofluorescent antibody technique (if) and elisa. among all samples positive by if and elisa, 52.9% were positive by latex test. this percentage was raised to 85.7% when only positive samples from patients with diarrhea were considered. we conclude that the latex tes ...19846099580
[virological and bacteriological research on the etiology of gastroenteritis in newborn calves].virologic and bacteriologic studies were carried out of samples taken from calves with clinical gastroenteritis and from calves that died at the age of 2 to 10 days. a total of 199 samples were investigated of calves on 48 cattle-breeding farms. in 69 of the cases (34.2 per cent) strains of escherichia coli were isolated, prevailing being those of the o8, o9, o15, o21, o26, o55, and o78 serogroups. three of the strains isolated from fecal samples of clinically affected calves possessed the k99 a ...19846099625
[antirotavirus iga antibody in nasal washes]. 19846099628
ultrastructural localization of rotavirus antigens using colloidal gold.colloidal gold was used to localize six of the ten known proteins of the simian rotavirus sa11 within infected cells by ultrastructural immunocytochemistry. monospecific or monoclonal antibodies to selected structural and nonstructural proteins were the primary antisera. the major outer capsid glycoprotein, vp7, was associated with nonenveloped particles, with particles de-enveloped by triton x-100 and with both nuclear and cytoplasmic inclusions. the protease-sensitive outer capsid protein, vp3 ...19846099654
comparative study of rotavirus strains of bovine and rabbit origin.newborn calves were susceptible to infection with a rotavirus strain isolated from rabbits and, conversely, rabbits 4-5 weeks of age became ill and some died when inoculated with the rotavirus strain of bovine origin. however, the latter isolate appeared to be more virulent than the rabbit isolate in either calves or rabbits. moreover, in reciprocal cross protection tests, carried out in calves and in rabbits, the rabbit rotavirus antiserum afforded only weak protection to challenge infection wi ...19846099786
[seroepidemiology of rotavirus in a population of children, goiânia, goiás, brazil]. 19846099909
role of rotavirus as the cause of acute paediatric diarrhoea in calcutta.of the 245 acute paediatric diarrhoea cases admitted to the infectious diseases hospital, calcutta between july, 1979 and june, 1981, rotavirus was detected in the faeces of 55 (22.4%) patients either as the sole aetiological agent or in association with other enteropathogens. children aged six months to two years were most commonly infected. the virus was detected throughout the year with higher incidence during the winter months of both years. the frequency of detection of major enteropathogen ...19846099923
increased "take" rate of oral rotavirus vaccine in infants after milk feeding. 19846147727
electron microscopic investigations of rotavirus morphogenesis in cell cultures.the replication of simian rotavirus sa11 in gmk cells and of bovine rotavirus in calf kidney cells was studied by electron microscopy. by 30 min post-inoculation (p.i.) sa11 virus was absorbed to the cell membrane in the absence of trypsin and became engulfed into the cell; clusters of viral particles were internalized also into cytoplasmic vacuoles. at 2 hr p.i., viral particles were seen in lysosomes and 6 hr p.i., the first progeny virus was found in the cisternae of endoplasmic reticulum (er ...19846147993
a bovine rotavirus developed as a candidate vaccine for use in humans. 19846098585
false positive rotazyme results. 19846141413
comparison of indirect double antibody and double antibody sandwich elisa techniques with latex agglutination test for the diagnosis of human rotavirus infection.94 faecal samples from infants and children suffering of acute gastroenteritis were investigated for rotavirus by indirect double antibody sandwich elisa kit (who, geneva), rotavirus elisa kit (dakopatts a/s, copenhagen) and rotalex latex-agglutination kit (orion diagnostica, helsinki). the elisa techniques gave almost identical results and seemed to be of same sensitivity and specificity. rotalex agglutination had an overall agreement of 88% with elisa. it is concluded that strongly positive re ...19846143499
protection of infants against rotavirus diarrhoea by rit 4237 attenuated bovine rotavirus strain vaccine.a randomised, double-blind, placebo-controlled trial was conducted to evaluate the ability of rit 4237 live attenuated bovine rotavirus (subgroup 1) vaccine strain to protect against natural rotavirus infection in children. 178 infants aged 8 to 11 months received a single oral dose of rit 4237 vaccine or placebo and were followed up serologically and clinically during a subgroup 2 rotavirus epidemic. no side-effects attributable to the vaccine were observed. during the 5 months' observation aft ...19846143964
an enveloped virus in stools of children and adults with gastroenteritis that resembles the breda virus of calves.pleomorphic virus-like particles about 100 nm in diameter with a fringe of closely applied peplomers (7-9 nm in length) were observed by electron microscopy in the stools of 20 children and adults with gastroenteritis. in most of the samples no other viral or bacterial pathogens were detected. in form and under immune electron microscopy these virus-like particles resembled the breda virus isolated from diarrhoeic calves. these objects may be a viral pathogen of humans.19846143978
waterborne outbreak of rotavirus diarrhoea in adults in china caused by a novel rotavirus.during two epidemics of acute diarrhoea in china in late 1982/early 1983, more than 12 000 adults in two coal mining districts were affected. the virus isolated from stool samples resembled a rotavirus morphologically. antigenically it lacked the group antigen shared by known rotaviruses. like other rotaviruses it had a double-stranded rna with 11 discrete segments, but the pattern of migration of the segments on polyacrylamide electrophoresis differed from those of other rotaviruses.19846144874
planar lattices in faeces of babies. 19846150267
seroepidemiological study of rotavirus infection in rural ghana.children in a village in rural ghana were found to have acquired antibody to rotavirus by the age of two years. the results of our study suggest that primary infection takes place early in infancy. fifty-six per cent of the three to 20-months-old had a four-fold rise in antibody levels during the six months follow-up period and 72.2% of them had one or more episodes of diarrhoea compared with 42.8% of those whose antibody levels stayed the same or decreased. the major epidemic season is consider ...19846210042
epidemiological aspects of porcine rotavirus infection in venezuela.the prevalence of porcine rotavirus infection in venezuela was studied from november 1980 to november 1981. rotaviruses were identified in 21.3 per cent of 286 diarrhoeic samples analysed by electron microscopy and in none of the 48 controls. rotaviruses were readily detected throughout the year, which may correspond to the absence of seasonal climatic variations in a tropical country such as venezuela. rotavirus infection was frequently observed in pigs that were two to six weeks old and was ra ...19846205426
sialodacryoadenitis in the rat: effects of immunosuppression on the course of the disease.eight-week-old outbred male and female crl:cd(sd)br rats were treated with prednisolone (pr) or cyclophosphamide (cy) and were inoculated intranasally with sialodacryoadenitis (sda) virus. the course of the disease was compared with nonimmunosuppressed, sda virus-inoculated rats of the same stock. criteria used to compare sda in the 3 groups, included histologic changes in salivary and lacrimal glands, immunofluorescent microscopy of paraffin-embedded tissues, serum amylase levels, and antibody ...19846208823
[viral diarrhea]. 19846209435
interferon response in colostrum-deprived newborn calves infected with bovine rotavirus: its possible role in the control of the pathogenicity.colostrum-deprived newborn calves were experimentally infected with cell-culture rotavirus. a similar process of infection was observed when the animals were inoculated immediately after birth or at the age of three days, with a corresponding delay in the onset of virus excretion and interferon production in the later case. with high doses of virus, interferon was produced very early and no symptoms were observed. with lower doses of virus, interferon production was delayed and preceded by a sev ...19846207759
monoclonal antibodies directed against different antigenic determinants of rotavirus.we have developed a series of monoclonal antibodies against the calf strain rit 4237 (subgroup 1) and the human strain 82-561 (subgroup 3) of rotavirus, both grown in tissue culture, and also against the human rotavirus 81-2162 (subgroup 2), extracted from a fecal specimen. a variety of different specificities was observed among these antibodies, namely, antibodies against group and subgroup determinants, as well as neutralizing antibodies. by using monoclonal antibodies against the subgroup ant ...19846202884
the molecular epidemiology of rotavirus gastroenteritis. 19846199812
serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization.a total of 16 different strains of rotavirus derived from seven mammalian species (four each from human and porcine species, two each from equine and simian species, and one each from canine and bovine species) and two avian species (one each from turkeys and chickens) were examined in plaque-reduction neutralization tests. seven antigenically distinct serotypes were established on the basis of a greater than or equal to 20-fold difference between titers of homologous and heterologous reciprocal ...19846202807
interaction of mucosal immune system and infections in infancy: implications in allergy.the immune responses to viral antigens and other environmental agents to which the mucosal surfaces of the neonate are exposed during the perinatal period and early infancy are intimately related to the mechanism of innate immunologic reactivity of the mother-and, possibly, to the immunologic elements in the products of lactation for a breast-feeding infant-and the nutritional homeostasis of the neonate. the role of these factors in protecting against pathogenicity of viral infections in the res ...19846239576
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