Publications

analysis of human rotavirus mixed electropherotypes.	mixed human rotavirus electropherotypes were detected in stool samples from patients with acute gastroenteritis in santiago, chile. these electropherotypes accounted for 10% of 149 samples studied. the finding of extra rna fragments with respect to the regular 11 genome segments suggests the possibility of simultaneous or sequential infection by more than one electropherotype in a single diarrhea event or occurrence of modification in the length of the rna segments during an infection. these pos ...	1983	6299944
an electron microscope study of wild human rotavirus replication in cell cultures.		1983	6302447
virus-specific immunity in neonatal and adult mouse rotavirus infection.	mouse rotavirus (epizootic diarrhea of infant mice) was used as a model to study the role of virus-specific immunity in infection and diarrheal disease. the distribution of viral antigen in intestinal tissues was determined by immunofluorescent staining with anti-simian rotavirus (sa-11) serum. the location and proportion of antigen-positive cells appeared to vary as a function of time postinfection and age of the animal at the time of infection. in animals infected at 1 and 7 days of age, antig ...	1983	6299952
[elaboration of a technic for the detection of rotaviruses in sewage].		1983	6303245
[comparative studies using the immunofluorescence technic in experimental transmissible gastroenteritis and rotavirus infection of piglets].		1983	6303246
[immunohistological detection of rotavirus antigen in the intestinal epithelium of children and adults].		1983	6303247
[electron-optic demonstration of rotaviruses in human small intestine epithelial cells].		1983	6303248
[model studies of the infectivity of aerosolized rotaviruses].		1983	6303249
[long-term study of the etiology of viral gastroenteritis in a children's institution].		1983	6303250
[cultivation of the human rotavirus].		1983	6303252
[morphological studies of rotavirus replication in cell cultures].		1983	6303253
[hemagglutinating properties of man and monkey rotaviruses].		1983	6303254
[cross infection with rotavirus of different species].		1983	6303255
[immunoelectron microscopy in the diagnosis of rotaviruses].		1983	6303256
[symposium on gastroenteritis-inducing viruses in man and animal. potsdam, december 1981].		1983	6303257
[rotavirus antibody determination by the neutralization test and electron microscopic detection of viruses in children with acute gastroenteritis].		1983	6303258
[diagnosis of rotavirus infections in infants and small children].		1983	6303259
[current possibilities in the routine diagnosis of rotavirus infections in the slovakian socialist republic].		1983	6303260
[epidemic acute gastroenteritis in newborn infants with the immunoelectronoptic detection of virus].		1983	6303261
[use of immunoelectroosmophoresis, agar gel precipitation and electron microscopy in the diagnosis of rotavirus infections in children and calves].		1983	6303262
[comparison of electron microscopy, gel electrophoresis and the immunofluorescence technic in the diagnosis of rotavirus infection in calves].		1983	6303263
derivation of neutralizing monoclonal antibodies against rotavirus.	monoclonal antibodies were derived against the sa11 simian, nic bovine, and wa human rotavirus strains and characterized by enzyme-linked immunosorbent assay, plaque neutralization, and hemagglutination inhibition. several strain sa11-specific antibodies were found to have neutralizing and hemagglutination-inhibiting capacity.	1983	6300449
dose effect on experimental reproduction of rotavirus diarrhoea in colostrum-deprived newborn calves.		1983	6302970
molecular cloning of double-stranded rna virus genomes.	genome double-stranded rnas isolated from purified human reovirus (serotype 3) and rotavirus (wa strain) were modified at the 3' termini by addition of oligo(c) approximately 15 with t4 rna ligase. these rnas were transcribed into cdna by oligo(dg)-primed reverse transcriptase and cloned after insertion into pbr322 at the pst i site. hybridization of plasmid-transformed escherichia coli rr1 colonies with 32p-labeled viral genome rnas demonstrated the presence of dna clones representative of each ...	1983	6300836
effects of tunicamycin on rotavirus morphogenesis and infectivity.	the functions of the two rotavirus glycoproteins were investigated by using tunicamycin and a variant of sa11 rotavirus having nonglycosylated vp7. results showed that glycosylation of vp7 is not required for normal viral morphogenesis and infectivity and suggested that the nonstructural glycoprotein is involved in assembly of the outer capsid.	1983	6298460
serum and intestinal immune response to rotavirus enteritis in children.	this study was designed to assess the serum and mucosal immune response to naturally acquired rotavirus enteritis in children. serum and duodenal secretions were collected 1 week and again 4 to 5 weeks after the onset of illness from 10 children. in two of these children, the procedure was repeated 12 to 15 months later. another 10 children with bacterial enteritis were studied as controls. the antibody response in serum included a significant elevation of rotavirus-specific immunoglobulin m (ig ...	1983	6301996
gene mapping of rotavirus double-stranded rna segments by northern blot hybridization: application to segments 7, 8, and 9.	cloned dna copies of double-stranded rna segments 7, 8, and 9 of uk bovine rotavirus were nick-translated with [alpha-(32)p]atp and hybridized to double-stranded rna of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o-aminophenylthioether paper. specific hybridization of the uk calf clones to the separated rna segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.	1983	6298465
propagation of bovine rotavirus by young dogs.	ten young dogs were experimentally infected twice with different isolates of bovine rotavirus and 2 uninfected dogs were kept in contact with them. none of the animals developed diarrhoea, but all of them excreted rotavirus in their faeces over a period of up to 10 days after each inoculation, as shown by counterimmunoelectro-osmophoresis and virus isolation. dogs may thus play a role in the epizootiology of rotavirus diarrhoea in calves. seroconversion occurred in 6 of the 10 infected dogs but ...	1983	6302138
diarrhea among infants and young children in canada: a longitudinal study in three northern communities.	diarrhea among neonates and their siblings was studied in 98 families living in winnipeg, manitoba, and in 31 native indian families and in 15 inuit (eskimo) families living in isolated settlements in northern canada. the rate of infection due to rotavirus in neonates was significantly higher and infection occurred more often in the first six months of life in the northern communities (range, 0.36 in winnipeg to 1.07 in eskimo point). no protective effect of breast-feeding was discerned, since i ...	1983	6302173
rotavirus persistence in cell cultures: selection of resistant cells in the presence of foetal calf serum.	the lincoln strain of bovine rotavirus, cytocidal for bovine au-bek cells, can establish in the same cell cultures in the presence of foetal calf serum (fcs) a persistent infection that depends on selection of highly resistant cells. in fact, after the induction of the carrier state only a small fraction of the cell population was infected. the parental and the carried viruses are not demonstrably different, the cultures are resistant to superinfection by the homologous virus, the cultures can b ...	1983	6302210
detection of rotavirus in handwashings of attendants of children with diarrhoea.		1983	6295543
purification of an outer capsid glycoprotein of neonatal calf diarrhea virus and preparation of its antisera.	an outer capsid glycoprotein, vp 7, was purified from a bovine rotavirus, neonatal calf diarrhea virus, by isoelectric focusing in glycerol gradients after disruption of the outer capsid. its isoelectric point was found to be about 4.5. guinea pigs were immunized with this vp 7 preparation. the antisera possessed both neutralizing and hemagglutination-inhibiting activities as well as complement-fixing activity, suggesting that vp 7 is a protein involved in hemagglutination and initiation of infe ...	1983	6295955
simple apparatus for collecting estuarine sediments and suspended solids to detect solids-associated virus.	laboratory trials of a new sampler for collection of estuarine sediment-associated virus resulted in a recovery effectiveness averaging 30% for two enteroviruses and rotavirus sa11. a minimal recovery potential of 54% was calculated when losses caused by virus concentration procedure inadequacies were excluded. both sediment-associated and suspended solids-associated viruses were collected with the sampler. recoveries of 61 and 60% poliovirus and rotavirus, respectively, were obtained from salt ...	1983	6297395
isolation and characterization of anti-rotavirus immunoglobulins secreted by cloned hybridoma cell lines.	five monoclonal hybridoma cell lines secreting antibodies against bovine rotavirus have been produced and four of them characterized by immunostaining of structural polypeptides electrophoretically transferred on to nitrocellulose sheets. three hybridomas appeared to be directed against the major structural polypeptide (vp39) of the virion. these three monoclonals cross-reacted with the major polypeptide of simian rotavirus and human rotavirus. a fourth hybridoma appeared to react specifically w ...	1983	6296301
rotavirus screening in adult cancer patients.		1983	6298949
epidemiology of rotavirus diarrhea in the highlands of papua, new guinea, in 1979, as revealed by electrophoresis of genome rna.	results of gel electrophoresis of rotavirus genome rna from feces of children in two provinces in papua, new guinea, suggest that the epidemiology of rotavirus infection in small communities with a total population of 3,000 may differ from that in urban or closely settled rural areas.	1983	6298273
oral administration of human rotavirus to volunteers: induction of illness and correlates of resistance.	four of 18 volunteers challenged orally with human rotavirus strain d (subgroup 2, serotype wa) developed a diarrheal illness two to four days after inoculation. viral shedding was detected in five of the 18 volunteers, whereas 12 (67%) developed serologic evidence of infection. two volunteers who developed diarrheal illness after the initial inoculation were given the same inoculum 19 months later; neither developed diarrhea, although one developed constitutional and gastrointestinal symptoms. ...	1983	6296243
identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation.	temperature-sensitive mutants of bovine rotavirus, uk compton strain, and rhesus monkey rotavirus, mmu18006 strain, were used to derive 16 reassortants by coinfection of ma104 cells. the parental viruses differed phenotypically in their neutralization specificity, their ability to hemagglutinate, and their requirement for exogenous trypsin for infectivity. when the reassortants were assayed for neutralization specificity and hemagglutination, four phenotypes were observed, indicating that these ...	1983	6299006
neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks.	to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ...	1983	6299483
isolation of a human pararotavirus.	during a survey of 350 different isolates of human rotaviruses, a single isolate with unique properties was obtained. unlike all rotaviruses tested this unique isolate failed to react in an elisa test with antibody directed against the group-specific rotavirus antigen. the morphology of this unusual virus isolate was identical in electron micrographs with other rotaviruses and the virus also contained 11 double-stranded rna segments characteristic of this virus group. the electrophoretic migrati ...	1983	6297152
development of vaccines against rotavirus disease.		1983	6318264
lifestyle and antiviral antibody studies among homosexual men in denmark.	two-hundred and fifty-nine male homosexuals (hs) of a large and a small danish town were studied for antibodies to various virus and their statistical relationship to sex habits, drug use and other lifestyle factors. prevalence rates against cytomegalovirus (cmv) were 86.7% of hs men from copenhagen and 73.3% of hs men from the smaller community of aarhus, against 30% of a control group. antibody prevalence in homosexuals was significantly related to number of years of homosexual activity (p les ...	1983	6318513
prevalence of bovine herpesvirus-1, parainfluenza-3, bovine rotavirus, bovine viral diarrhea, bovine adenovirus-7, bovine leukemia virus and bluetongue virus antibodies in cattle in mexico.		1983	6316006
detection of antibody to epizootic diarrhea of infant mice (edim) virus.	cultured mouse liver cells containing either eipzootic diarrhea of infant mice viral antigen or nebraska calf diarrhea vaccine virus antigen were equally effective for detecting serum antibody to epizootic diarrhea of infant mice virus by immunofluorescence. the presence of rotavirus in infant mouse intestinal suspensions used as cell culture inocula was confirmed by polyacrylamide gel electrophoresis of the double-stranded rna viral genome. when the immunofluorescence assay was tested in a limi ...	1983	6316027
cytokine-stimulated human natural killer cytotoxicity: response to rotavirus-infected cells.	the ability of rotavirus-infected cells to stimulate leukocytes to release a cytokine which enhanced the subsequent leukocyte cytotoxicity to a second set of [51cr] labeled rotavirus-infected cells was analyzed. human interferon increased leukocyte cytotoxicity to simian rotavirus (sa-11)-infected target cells. similarly, 11 of 12 supernates of sa-11-stimulated peripheral blood leukocyte cultures increased the killing of sa-11-infected cells (p less than 0.005). this resulted in a calculated cyt ...	1983	6316244
[rotavirus infection in infants with acute diarrhea. clinical and epidemiologic aspects].		1983	6316454
[clinical symptoms and therapeutic possibilities in rotavirus infections].	author reviews his personal experience on 189 cases of gastroenterocolitis produced by rotavirus during a two year period (1980-81). after exposing diagnostic and clinical features, he brings into consideration future possible therapy of this viral infection.	1983	6318619
canine viral enteritis.	canine viral enteritis should be suspected in dogs with an acute onset of vomiting and diarrhea, especially in puppies and where several animals are affected simultaneously. definitive diagnosis requires laboratory confirmation, most often detection of viral particles in the stool. no diagnostic test is entirely specific or absolutely sensitive, however, and laboratory findings should be weighed accordingly. immunization is the key to successful control. effective vaccines for canine parvovirus ...	1983	6316616
[attempt at the experimental reproduction of rotavirus diarrhea in newborn calves deprived of colostrum].	colostrum-deprived newborn calves were orally inoculated with different doses of cell-culture bovine rotavirus or with bacterium-free filtrates of calf stools containing rotavirus. none of the animals that received high doses of cell-culture rotavirus developed diarrhoea or any other clinical sign, although all of them excreted virus for several days and produced specific antibodies; calves inoculated with lower doses of cell-culture virus or with stool filtrates showed a transient diarrhoea 48 ...	1983	6318646
longitudinal survey of rotavirus infection in calves.	a longitudinal survey of rotavirus infection in heifer calves was carried out on a closed friesian dairy herd over two successive calving seasons. rotavirus was detected by electron microscopy in the faeces of 45 of 57 (79 per cent) calves examined. on average the virus was first detected at 6.1 days of age. clinically the disease associated with rotavirus infection was of mild to moderate severity. only one infected calf required intravenous fluid therapy. diarrhoea or excretion of abnormal fae ...	1983	6316619
isolation and genetic characterization of temperature-sensitive mutants that define five additional recombination groups in simian rotavirus sa11.	nineteen independent temperature-sensitive (ts) mutants were isolated from sa11 following mutagenesis with proflavin or 5-azacytidine. fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. five of the ts mutants defined five recombination groups (f, g, h, i, and j) that had not been previously identified. thus, 10 of the 11 expected mutant groups have been identified in sa11. the prototype mutants of the 10 mutant groups were tested for re ...	1983	6316640
isolation of new serotypes of bovine rotavirus. brief report.	two cytopathogenic bovine rotavirus strains were isolated. the infectivity of both isolates was relatively stable at ph 3.0 and resistant to ether. their replication was not affected by 5-iodo-2'-deoxyuridine. the new isolates shared immunofluorescent antigen with the lincoln strain of bovine rotavirus. by neutralization test, however, these strains were clearly distinguished from one another.	1983	6316874
antibody response in rabbits following oral administration of human rota-, calici- and adenovirus. brief report.	rota-, calici-, adeno- and astrovirus extracted from human faeces were administered orally to eight rabbits. antibody rise was demonstrated in some of the animals against rota-, calici- and adenovirus. clinical illness or virus excretion was not observed.	1983	6316877
rotavirus as a cause of severe gastroenteritis in adults.	rotavirus was identified as the only etiological agent in 5% of adults (28 of 526) with diarrhea who were admitted to bamrasnaradura hospital in nonthaburi, thailand, during a 1-year period. infection was determined by detection of rotavirus in diarrheal stools by enzyme-linked immunosorbent assay accompanied by a greater than fourfold rise in serum complement fixation and radioimmunoassay antibody titers to rotavirus. adults with clinical rotavirus infections were as severely ill as patients wi ...	1983	6313748
virological survey of children in nyeri and mombasa. monthly survey of rotavirus in faeces.		1983	6317333
ultrastructural evidence for the cellular uptake of rotavirus by endocytosis.	the ultrastructural aspects of rotavirus sa11 infection were studied in ma104 cells, with particular attention directed to the early stages of virus adsorption and penetration. within the first few minutes postadsorption, rotavirus was seen at the plasma membrane, associated with coated pits and coated vesicles, indicating that sa11 virus is taken into the cell by adsorptive or receptor-mediated endocytosis. multiple virus particles were seen, beginning at 1 h and continuing throughout infection ...	1983	6317607
protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge.	studies were performed to evaluate the potential use of the bovine rit 4237 rotavirus strain as a vaccine candidate against infection with human rotaviruses. initial experiments revealed that colostrum-deprived piglets were susceptible to infection with several human strains, except for those belonging to subgroup 1. subsequently, different immunization procedures with rit 4237 were studied in this animal model. it was found that a two-dose administration, either given intramuscularly (twice) or ...	1983	6317767
coding assignment and nucleotide sequence of simian rotavirus sa11 gene segment 10: location of glycosylation sites suggests that the signal peptide is not cleaved.	a cloned dna copy of simian rotavirus sa11 genomic segment 10 was used to confirm the assignment of the nonstructural glycoprotein ncvp5 to this gene. determination of the nucleotide sequence for gene 10 indicated that ncvp5 is 175 amino acids in length and has an n-terminal hydrophobic region with the characteristics of a signal sequence for membrane translocation. unexpectedly, this region was also the location for the only two potential glycosylation sites within the molecule, asparagine resi ...	1983	6312090
necrotizing enterocolitis in full-term newborns.	during a 17-month period, 19 full-term newborn infants were hospitalized for necrotizing enterocolitis (nec). 10 of these infants had no apparent risk factors. only 7 suffered acute fetal distress. 16 of the 19 infants were born in the same hospital. toxicological and bacteriological investigations of possible cause revealed no specific agent. virological analysis of 8 stool samples revealed the presence of corona virus in 5 of the 8.	1983	6313080
a study of cytopathic rotavirus strains isolated from calves with acute enteritis.	nine cytopathic bovine rotavirus strains were isolated in ma-104 cell cultures from fecal specimens of dairy calves suffering from diarrhea. isolation of the virus was accomplished from three outbreaks which occurred on dairy farms located in central and southern italy. fecal suspensions were treated with a high concentration (1000 micrograms/ml) of trypsin, and inoculated into ma-104 cell cultures grown out in eagle's minimum essential medium (mem) containing 5 micrograms/ml of the enzyme. cyto ...	1983	6313289
rotaviruses: a review.		1983	6313296
association of rotavirus infection with kawasaki syndrome.		1983	6309994
induction of cross-reactive serum neutralizing antibody to human rotavirus in calves after in utero administration of bovine rotavirus.	sera from calves infected in utero or postnatally with bovine rotavirus ncdv or postnatally with human rotavirus d (serotype 1) were tested by plaque reduction neutralization assay for antibody to bovine rotavirus and to three serotypes of human rotavirus. homologous antibody developed in all animals, but antibody to heterologous rotaviruses developed mainly in animals exposed in utero to bovine rotavirus. the development of heterologous antibody may explain the immunological implications for cr ...	1983	6313744
an atypical rotavirus detected in a child with gastroenteritis in rio de janeiro, brazil.		1983	6318014
detection of rotavirus in respiratory secretions of children with pneumonia.		1983	6312006
isolation and characterization of an equine rotavirus.	a rotavirus, designated as the h-1 strain, was isolated from a diarrheic foal in primary african green monkey kidney cells and ma104 cells. this cell culture-adapted strain hemagglutinated erythrocytes of human group o, rhesus monkeys, guinea pigs, and sheep. it was found to be similar, if not identical, to porcine rotaviruses (strains osu, ee, and a-580) by plaque reduction neutralization and hemagglutination inhibition tests, and, in addition, it was found to belong to subgroup 1. this equine ...	1983	6313746
survival and detection of rotaviruses on environmental surfaces in day care centers.	previously, we demonstrated that children in day care centers commonly experience diarrhea due to rotavirus, giardia, and bacterial pathogens. multiple agents frequently coexist, and the environment is heavily contaminated with enteric bacteria during outbreaks. a study of environmental surface contamination with rotavirus was performed during three non-outbreak periods. of 25 samples collected from environmental surfaces and teachers hands at a day care center, 4 (16%) were positive for rotavir ...	1983	6314896
[rotavirus infections in a neonatal pathology unit].	from february to june 1981, 38 newborn babies admitted to a special care unit were examined to determine the incidence of rotavirus infections. fecal specimens were collected from all babies on the fifth day after birth and thereafter weekly. the stool samples were examined with an immunoenzymatic test. serum was collected from the newborn babies and from their mothers three days after delivery. thereafter more serum was collected from the babies at intervals. serum was also collected from the h ...	1983	6315031
concentration of rotavirus and enteroviruses from blue crabs (callinectes sapidus).	a simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. virus was separated from tissue homogenates at ph 9.5, concentrated by adsorption to protein precipitates at ph 3.5, and recovered by elution of precipitates at ph 9.2. test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. cat-floc precipitation was used to remove sample toxicity for cell cultures. recovery effectiveness averaged 52% with poliovirus 1, echoviru ...	1983	6318667
[demonstration of an experimentally induced antigenic drift in rotaviruses].		1983	6318682
spread of rotavirus within families: a community based study.	the spread of rotavirus infection was studied over four weeks in a sample of 28 families exposed to a child with rotavirus infection. the results showed a high incidence of intrafamilial infection, with 46% of members of these families developing rotavirus infections compared with none in another series of 18 families. children in the families with an index case were more frequently affected than adults: 75% of the children developed rotavirus infection but only 33% of the adults. children tende ...	1983	6309316
prevalence of neutralizing antibodies against a candidate rotavirus vaccine in adults.	because of the morbidity and mortality from infection with rotaviruses in children under 2 years, in many parts of the world but especially in developing countries, an urgent need for a vaccine has been recognized. at present, a vaccine candidate of bovine origin, the rit 4237 strain vaccine developed from a strain received from mebus et al., is available. this strain has been shown to induce cross protection by challenge of colostrum deprived piglets with human rotavirus strains. before testing ...	1983	6307775
studies in volunteers with human rotaviruses.		1983	6307776
effect of feeding human milk on nosocomial rotavirus infections in an infants ward.	children aged 1-15 months hospitalized for different respiratory diseases (including otitis media) at an infants ward frequently developed diarrhoea during their stay in the clinic. bacteriological examinations of stool samples were usually negative but rotavirus excretion was frequent according to elisa tests. children fed with human milk are assumed to be less susceptible to rotavirus infections due to the protection by maternal antibody. in view of preventive measures for avoiding rotavirus n ...	1983	6307777
[studies on the use of staphylococcus protein a solid phase immune electron microscopy to increase the detection rate of rotavirus].		1983	6305465
clinical, virological and serological evaluation of the efficacy of peroral live rotavirus vaccination in calves kept under normal husbandry conditions.		1983	6306961
the effect of relative humidity and temperature on the survival of bovine rotavirus in aerosol.	the effect of relative humidity and temperature on the survival of bovine rotavirus in airborne particles was investigated. bovine rotavirus was found to be stable at low and high relative humidity; infectivity was lost more rapidly at high temperature than at lower temperature.	1983	6307226
detection of antigenically distinct rotaviruses from infants.	antigenically distinct rotaviruses, i.e., viruses morphologically identical to conventional rotaviruses by electron microscopy, yet lacking the common group antigen(s) detected by an enzyme-linked immunosorbent assay, were found in 2 of 51 fecal samples from bulgarian infants with rotavirus gastroenteritis. these antigenically distinct viruses contained 11 segments of double-stranded rna, but they demonstrated a unique rna migration profile after electrophoresis of the genome rna in polyacrylami ...	1983	6307873
[the viral incidence in childhood diarrhea].		1983	6309062
passive immunity to bovine rotavirus in newborn calves fed colostrum supplements from immunized or nonimmunized cows.	colostrum was collected and pooled from each of five cows in three experimental groups: group i cows received intramuscular and intramammary inoculations of adjuvanted modified live ohio agricultural research and development center rotavirus vaccine; group ii cows were injected intramuscularly with a commercial modified-live rota-coronavirus vaccine; and group iii cows were uninoculated controls. pooled colostrum from group i cows had higher (p less than 0.05) enzyme-linked immunosorbent assay ( ...	1983	6309660
epidemiologic background for the need of rotavirus vaccine in finland. preliminary experience of rit 4237 strain of live attenuated rotavirus vaccine in adults.	epidemiologic evidence from finland indicates a dramatic change in the seasonal pattern of acute diarrhoea over the past 30 years. while in the 1950's the majority of cases occurred in late summer the seasonal peak in the 1970's was in winter and spring, coinciding with the prevalence of rotavirus infections. it is not possible to determine retrospectively the aetiologic agents involved in the summer diarrhoea, but epec may have been one of them, as epec are quite rare in finland today compared ...	1983	6307778
vaccination of cows with a combined rotavirus/enterotoxigenic "e. coli" k99 vaccine to protect newborn calves against diarrhoea.	the protective effect of a combined rotavirus/enterotoxigenic e. coli k99 vaccine against naturally occurring neonatal diarrhoea was evaluated in two dairy and two beef herds. before vaccination, frequency of diarrhoea in these herds was 46% up to 60% with losses of 7% to 20%. after vaccination of pregnant cows and heifers mild diarrhoea occurred in 14% to 20% of their calves, one out of 144 calves died with enteric symptoms. in 13 out of 26 calves of nonvaccinated controls where diarrhoea was n ...	1983	6307779
passive protection of newborn calves against rotavirus by vaccination of their dams.	the rotavirus is considered as being one of the major causes of acute gastroenteritis; it occurs in cattle farms where, either by itself or in association with other pathogenic agents, it is responsible for a high percentage of neonatal morbidity and mortality. over two consecutive years, field trials were done using inactivated rotavirus vaccine in oily excipient inoculated to pregnant cows. results obtained prove a significant increase in the rate of colostral antibodies and a very real prolon ...	1983	6307780
infection of baboons ("papio cynocephalus") with rotavirus (sa11).	a recent survey of nonhuman primate sera indicated that antibody to a rotavirus (sa11) was prevalent among a wide spectrum of animals. both new and old world species were found with antibody, many with surprisingly high titers (1:320). whether or not infection per se was due to sa11 or an antigenically closely related agent could not be determined by that study; however, it is apparent from this study as well as from a survey of the literature that natural as well as experimental infection of no ...	1983	6307781
characterization of rotaviral rna isolated from children with gastroenteritis in two hospitals in rotterdam.	rotavirus rna was extracted from stools collected from children with gastroenteritis in two hospitals in rotterdam. electrophoresis of the rnas showed that at least 19 different electropherotypes were isolated from stools collected in the period from 1977 to 1982. it was possible to identify the index case in some hospital-acquired cases of rotaviral gastroenteritis.	1983	6311968
characteristics of the cell-mediated immune response to rotavirus in suckling mice.	the kinetics of rotavirus specific cell mediated immunity and rotavirus infection were compared in infected and uninfected inbred balb-c mice. lymphocytes transformation (ltf) in spleen cells exposed to varying doses of mouse rotavirus (mrv) and control antigens was carried out at intervals up to 60 days following infection. rotavirus infection was monitored by the detection of rotavirus antigen in isolated small intestinal enterocytes using direct immunofluorescent assay. the severity of diarrh ...	1983	6307782
unreliability of rotazyme elisa test in neonates.		1983	6308197
detection of rotavirus in horses with and without diarrhea by electron microscopy and rotazyme test.	a total of 142 equine fecal samples (93 field fecal and 49 experimental fecal specimens) were examined for rotavirus using direct electron microscopy (em) and the rotazyme test. eighty-six stool specimens were diarrhea samples. the rotazyme test sensitivity and accuracy as compared to em was determined by the visual (color reaction) and spectrophotometric methods. the overall agreement was 94.8% and 92.3% between em and rotazyme visual and spectrophotometric methods, respectively when suspect re ...	1983	6309476
characteristics and analysis of electropherotypes of human rotavirus isolated in chile.	electropherotypes of human rotavirus from stool samples of hospitalized and ambulatory infants with acute diarrhea were analysed. thirty-two different electropherotypes were observed in 142 cases in which the viral genome rna was characterized. the seasonal distribution of the electropherotypes, from 1979 to 1981, showed that the same electropherotypes were detected throughout the period of study while others were found only occasionally. electropherotypes were also analyzed depending on whether ...	1983	6309997
[incidence of viral particles in children with acute or protracted diarrhea, seen at the hospital das clinicas of the faculdade de medicina de botucatu from february 1980 to february 1981].		1983	6310721
human rotaviruses and genome rna.		1983	6309998
rotavirus-associated traveler's diarrhea: neutralizing antibody in asymptomatic infections.	the sera of patients with traveler's diarrhea were assayed for rotavirus-specific immunoglobulins in enzyme-linked immunosorbent and micro-neutralization assays. the antigenically related simian rotavirus sa-11 was used in both assays. although similar titers of rotavirus-specific binding immunoglobulin g and a were observed in the sera of both symptomatic and asymptomatic subjects, clinical illness appeared to correlate with the failure to detect increased (greater than or equal to fourfold) ti ...	1983	6307883
an outbreak of rotavirus-associated neonatal necrotizing enterocolitis.	an outbreak of necrotizing enterocolitis and hemorrhagic gastroenteritis occurred in two nurseries during 25 days in august 1982. eleven of the 40 patients in these nurseries during that time developed disease (attack rate 27.5%). in seven of the 10 patients with gastrointestinal disease, stool samples tested for human rotavirus were positive by elisa, whereas in 20 unaffected infants, no stools tested demonstrated hrv (p = 0.0001). eleven staff members had serologic evidence of recent hrv infec ...	1983	6310072
response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization.	sera from (i) gnotobiotic balb/c, cd-1, and cfw mice and (ii) conventional balb/c mice were evaluated by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization, using the wa, sa-11, and wc-3 (bovine) strains of rotavirus as the detecting antigens. the gnotobiotic mice had no antirotavirus antibody detectable by radioimmunoprecipitation and no neutralizing antibody at a dilution of 1:50 by plaque reduction neutralization. all sera from the conventional mice had rotavirus- ...	1983	6311747
isolation, propagation, and characterization of a second equine rotavirus serotype.	a rotavirus designated strain h-2 was isolated in primary african green monkey kidney cells from a foal with diarrhea. this cell culture-adapted strain was found to be similar, if not identical, to simian rotavirus (strains mmu18006 and sa-11) and canine rotavirus (strain cu-1) and, in addition, demonstrated a one-way antigenic relationship with five human rotavirus strains (p, b, no. 14, no. 15, and yo) of the third human rotavirus serotype by the plaque reduction neutralization test. this is t ...	1983	6309657
pediatric viral gastroenteritis during eight years of study.	during the period january 1974 through july 1982, fecal samples from 1,537 pediatric inpatients with gastroenteritis were tested for enteric viruses by electron microscopic and rotavirus enzyme-linked immunosorbent assay techniques. rotaviruses were detected in 34.5% of these patients, enteric adenoviruses were detected in 4.7%, approximately 27-nm viruses were detected in 1.6%, and at least one of these agents was found in 40.1% of the study subjects. three infections were by an apparently new ...	1983	6309901
infectious gastroenteritis in norfolk island and recovery of viruses from drinking water.	a high incidence of gastroenteritis in both islanders and tourists has been recorded in recent years on norfolk island--a popular tourist resort for australians and new zealanders. no bacterial cause has been found. however, electron microscopic examination of 28 faecal specimens revealed viruses associated with gastroenteritis in 21 (75%). no viruses were isolated in cell cultures. bore water is used for drinking purposes on the island and 32 samples from 15 bores were examined for viruses by e ...	1983	6309956
use of electrophoresis of rna from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery.	during two nosocomial outbreaks in a tertiary care nursery, 22 of 102 infants had infections due to human rotavirus (hrv). the time sequence and proximity of the infected infants in the nursery rooms had suggested infant-to-infant spread of a single hrv strain in each outbreak. polyacrylamide gel electrophoresis of virus permitted demonstration of four different hrv electropherotypes (strains) during the first outbreak period and seven during the second. one of the strains was detected during bo ...	1983	6309996
completion of the gene coding assignments of sa11 rotavirus: gene products of segments 7, 8, and 9.	improved fractionation of double-stranded rna segments 7, 8, and 9 of simian rotavirus sa11 has permitted their isolation and individual translation in vitro. segment 7 codes for p31 (ns2), segment 8 codes for p33 (ns1), and the segment 9 gene product resembles the gp34 precursor observed in sa11 virus-infected cells. in vitro glycosylation of translation products of segments 5 and 10 was also observed.	1983	6310157
molecular characterization of rotaviruses with distinct group antigens.	comparative serological and nucleic acid studies were carried out on rotavirus isolates, with and without the original group antigen. indirect immunofluorescence allowed the atypical isolates to be divided into two groups each with its own distinct group antigen hence giving a total of at least three distinct rotavirus groups. genome profile analysis of the atypical isolates again divided them into two groups which corresponded with those obtained by immunofluorescence and within which the overa ...	1983	6311947