TitleAbstractYear(sorted ascending)
letter: "rotavirus" in stored specimens of faecal extracts. 19744138251
letter: "rotavirus" in gastroenteritis. 19744143001
epidemic viral enteritis in a long-stay children's ward.two outbreaks of acute gastroenteritis occurred in 1974 in a long-stay children's ward. electron microscopy demonstrated rotaviruses in faeces from the affected children in the first outbreak, and adenoviruses in faeces from affected children and a nurse in the second outbreak. the illness in both outbreaks was very mild; but the diarrhoea associated with rotavirus infection usually lasted 5-8 days (in one patient it lasted for 28 days) and sometimes started with vomiting; whereas the adenovirus ...197546358
importance of a new virus in acute sporadic enteritis in a year-long survey of children admitted with acute enteritis to hospital in melbourne, australia, an aetiological agent was found in approximately 75% of patients. during most months of the year a newly described virus belonging to the family reoviridae was detected by electron microscopy of faecal extracts from more than 50% of patients. recovery of this virus reached a peak (73%) during the winter. all age-group up to five years of age were susceptible to infection, which was occasionally f ...197546387
editorial: rotaviruses of man and animals. 197546393
letter: rotaviruses of man and animals. 197547983
letter: rotavirus infections in a maternity unit. 197549677
letter: rotavirus infections in obstetric hospitals. 197549709
rotavirus enteritis in the west midlands during 1974.during 1974 in the west midlands of england, 38% of children less than 6 years old with enteritis were excreting rotaviruses. children aged from 6 months to 3 years were those most commonly infected. rotavirus infections were most common during winter with only a few sporadic cases during summer. a possible pathogen was detected in 59% of patients examined.197549794
letter: rotavirus infections in neonates. 197551252
letter: isolation of human rotavirus using human embryonic gut monolayers. 197553455
in-vitro detection of human rotaviruses. 197578193
neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. 1975172192
characterisation of a rotavirus.20b. 1975173999
[infantile gastroenteritis: a new viral agent, the rotavirus]. 19751101185
transmission of human rotaviruses to gnotobiotic piglets.faecal filtrates containing rotavirus particles, from children with acute infectious diarrhoea, were inoculated intranasally into gnotobiotic piglets. the piglets developed no symptoms, but birus was readily found by electron microscopy in their faeces during three serial passages. among 11 piglets tested 3 weeks after inoculation of virus, all had developed fluorescent antibodies against tissue-culture-adapted calf rotavirus but only two had neutralising antibody. growth of human rotavirus did ...19751107554
reovirus and rotavirus infections. 1975220624
reovirus-like agent (rotavirus) from lambs.rotavirus particles were demonstrated by electron microscopy in the feces of lambs with diarrhea. rotavirus antigen was synthesized in cell cultures infected with filtrates of the diarrheic feces, but the virus was not adapted to grow serially in cell cultures. an antigenic relationship between rotaviruses from lambs, pigs, and calves was demonstrated by immunofluorescence. colostrum-deprived lambs were infected with the lamb rotavirus, and the virus was passaged in lambs. viral replication occu ...1976793991
characteristics of the genome of human infantile enteritis virus (rotavirus).thermal denaturation studies and polyacrylamide gel electrophoretic analysis have shown that the genome of human infantile enteritis virus (human rotavirus) consists of approximately 11 double-stranded segments. differences in the molecular weights of some of the corresponding genome segments are apparent between different isolates of the virus.1976820876
[rotavirus as a cause of acute gastroenteritis]. 1976824559
the morphogenesis of a cytopathic bovine rotavirus in madin-darby bovine kidney cells.the morphogenesis of a cytopathic bovine rotavirus isolate was examined in mdbk cells. distension of cisternae of the rough endoplasmic reticulum was seen 14 h post-infection (p.i.), but few virus particles were present. many virus particles were observed within distended cisternae of the endoplasmic reticulum 22 h p.i., and matrices of viroplasm were found close to developing virus particles. the virus particles measured 68 to 72 nm in diam. and consisted of an electron-dense nucleoid surrounde ...1976826605
letter: rotavirus as a cause of gastro-enteritis in black south african infants. 1976959923
morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals.the reovirus-like particles present in the feces of young pigs and foals with acute enteritis and the virus causing epizootic diarrhea of infant mice were found to be indistinguishable morphologically from each other, from the south african sa. 11 and "o" viruses, and from the rotaviruses of children and calves. the inner capsid layer of each of these viruses reacted seriologically with sera of children, calves, mice, piglets, and foals convalescent from infection with their respective rotavirus ...1976965097
isolation of a rotavirus from deer. 1976997186
rotavirus infection in lambs: studies on passive protection.gnotobiotic lambs were protected against rotavirus infection by the presence in the gut at the time of infection of colostrum or serum containing antibodies to rotavirus. this protection was observed even when passively-acquired antibody was not present in the serum of the infected lamb. infection under these conditions may have conferred immunity to subsequent challenge.1976999520
winter diarrhoea and rotaviruses in the winter fewer bacterial pathogens are isolated from patients with gastro-enteritis than in the summer. the incidence of rotavirus infection is, however, at its greatest during the winter months and the virus is rarely found in cases of gastro-enteritis which occur during the warm season. the clinical pattern in winter diarrhoea is characteristically severe and acute but there has been no mortality or cross-infection.19761006454
[rotavirus infection in calves (author's transl)].several laboratory workers have demonstrated a viral agent, suspected of playing an important role in the aetiology of calf diarrhoea during the first weeks of life. based on its morphology this agent was designated a reo-like virus. later the name rotavirus was introduced. rotaviruses were proved experimentally to induce diarrhoea in calves. usually the course of the disease after similar experimental infection is mild, but could become worse with secondary bacterial infections. the available i ...19761013979
[in vitro culture of a rotavirus associated with neonatal calf diarrhea].about fifty diarrheic fecal samples in which the calf rotavirus was present in variable quantities were passed in tissue cultures and the ability of the virus to replicate was analysed under different experimental conditions by means of an indirect immunoflourescent test. several cell species were shown to be susceptible to the virus but the best results were obtained in primary or secondary cultures of embryonic calf kidney cells in presence of maintainance medium containing 2 to 4 p. 100 foeta ...19761028381
a rotavirus in lambs with diarrhoea.a reovirus-like agent was identified from an outbreak of enteritis in young lambs. from its morphology and immunological relationship with calf rotavirus, it was concluded that it was a rotavirus which infects lambs.19761257621
rotaviruses and acute gastroenteritis of infants and means of electron microscopy and ultracentrifugation techniques rotavirus-like particles have been found in 84 of 269 specimens of faeces collected from infants and young children admitted to the royal alexandra hospital for children, camperdown, n.s.w. during the usual winter outbreak of infantile gastroenteritis. morphologically intact viruses were also found in faecal specimens stored at -20 degrees c for up to nine years. rotaviruses were found in specimens collected from five newborn inf ...19761263949
an epidemic of diarrhoea in human neonates involving a reovirus-like agent and 'enteropathogenic' serotypes of escherichia coli.during december 1974, an epidemic of diarrhoea occurred in the royal children's hospital, melbourne, in a ward caring for neonates with acute or chronic medical and surgical problems. electron microscopy of diarrhoeal faeces revealed a reovirus-like particle ('duovirus' or 'rotavirus') known to cause acute enteritis in older children. this virus is considered to have been primarily involved in the aetiology of the epidemic. in addition, three 'enteropathogenic' serotypes of escherichia coli were ...1976175099
a reovirus-like agent (rotavirus) in gastroenteritis of children. virus detection and serological studies.a reovirus-like agent (rotavirus) was detected in 26 children (44%) when fecal specimens from 59 children with acute gastroenteritis were examined by electron microscopy. no rotavirus was detected in the feces of 49 children with other diseases. sera from the acute and the convalescent phase from 40 children with acute gastroenteritis and from 18 other patients were examined for antibodies against a bovine rotavirus by an indirect fluorescent antibody test. 26 of the patients with gastroenteriti ...1976178049
characterization of two particle types of calf rotavirus.two distinct types of rotavirus particle were isolated in caesium chloride density gradients. the higher density particle banded at 1-38 g/ml and measured 55 +/- 0-4 nm in diameter while the less dense particle banded at 1-36 g/ml, measured 66 +/- 0-4 nm in diameter and appeared to possess an extra outer capsid layer. some forms intermediate between the two types were seen. since the two particles had a similar but not identical morphology and polypeptide composition, they were considered to be ...1976180243
the isolation of reovirus-like agents (rota-viruses) from acute gastroenteritis of piglets.isolations of reovirus-like agents (rotaviruses) were made from nine of 23 outbreaks of piglet diarrhoea on different farms and from both weaned and unweaned piglets. the viruses were shown to be morphologically and anti-genically similar to the rotaviruses of children and calves. gnotobiotig piglets given intranasal inoculations of five different isolates developed acute gastroenteritis, and the virus was re-isolated from the faeces or intestinal contents. the piglet virus was not adapted to re ...1976180294
[responsibility of the rotavirus in infantile diarrheas]. 1976180494
gnotobiotic piglets experimentally infected with neonatal calf diarrhoea reovirus-like agent (rotavirus).gnotobiotic piglets were infected with a strain of rotavirus that had been isolated from a calf and passaged four times in piglets. the resulting disease was studied by light and electron microscopy at 21, 44 and 68 h after inoculation. in the small intestine infection caused desquamation of the epithelial cells of the villi resulting in severe stunting. there was severe damage to microvilli and accumulation of lipid within the cytoplasm. virus particles were seen in epithelial cells covering th ...1976180653
comparison of methods for diagnosis of rotavirus infection of calves. 1976180654
isolation of a cytopathic calf rotavirus.the isolation and serial passage in secondary and continuous cultures of calf kidney cells of a cytopathic calf rotavirus is described.1976181805
characterization of pig rotavirus rna.pig rotavirus was purified from faeces. the rna from this virus was resistant to pancreatic ribonuclease, indicating that it is double-stranded. when electrophoresed on polyacrylamide-agarose gels, pig rotavirus rna migrated as 9 bands comprised of 11 or 12 rna segments with a total mol. wt. of approx. 11 x 10(6). co-electrophoresis experiments revealed that the rnas from the pig virus and two isolates of the calf rotavirus were indistinguishable.1976185325
studies on rotavirus infection and diarrhoea in young calves.the occurrence of diarrhoea in calves was monitored during the first three weeks of life. calves fed amounts of colostrum sufficient to produce serum ig levels in excess of 30 mg per ml did not develop diarrhoea, whereas calves fed less colostrum did. rotaviruses and mycoplasma-like particles were observed in the faeces of calves with and without diarrhoea. the epidemiology of rotavirus infection in calves is discussed.1976185778
rotavirus in calves... 1976185781
rotavirus and rabbits. 1976185782
reovirus-like agent (rotavirus) associated with neonatal calf gastroenteritis in france.a reo-like virus (calf rotavirus) was shown to be associated with cases of neonatal calf diarrhea in france. the virus could be detected in more than 50 p. 100 of diarrheic fecal samples, while it was practically absent in control samples originating from healthy calves that had never had diarrhea. the results obtained by electron microscopy and immunofluorescent studies indicate that the virus is closely related or identical to the agent isolated originally in the united states.1976185934
implications of recent virological researches.rotaviruses (duoviruses) can be found in more than half the cases of acute diarrhoea in children up to the age of six or seven. about that age almost everyone has antibodies to them. second infections occur and may not be as rare as laboratory findings so far suggest. very young infants sometimes get subclinical disease-the effect of maternal antibody transmitted across the placenta? very similar viruses, all possessing a common antigen detectable by immunofluorescence, are known to infect and/o ...1976186237
pathogenic rotaviruses isolated from pigs and calves.rotavirus is commonly isolated from diarrhoeic calves and pigs. bacterium-free faecal filtrates containing rotavirus from five different outbreaks of disease in calves all caused diarrhoea and clinical illness in gnotobiotic calves and five different isolates from pigs were inoculated into gnotobiotic pigs with similar results. the author was unsuccessful in finding an avirulent strain although one of the calf isolates was from a non-diarrhoeic calf. the laboratory strain of calf virus retained ...1976186238
recent advances in the aetiology of viral gastroenteritis.studies with the human reovirus-like (hrvl) agnet, also designated rotavirus and duovirus, have revealed that it is a major aetiological agent of diarrhoea of infants and young children in many parts of the world. in a study of patients admitted with a diarrhoeal illness to the children's hospital of the district of columbia in the united states from january 1974 to june 1975, it was found that half of the patients studied by both virus shedding (by electron microscopy) and serological (compleme ...1976186239
experimental rotavirus infection in lambs. 1976186496
rotavirus infections. 1976186931
[rotaviruses as pathogen organism of acute gastroenteritis in adults]. 1976187550
virus-specific igm antibodies in acute gastroenteritis due to a reovirus-like agent (rotavirus).62 serum samples from 24 patients with rotavirus gastroenteritis were tested for igm antibodies against a bovine rotavirus by an indirect fluorescent antibody technique. igm antibodies were detected in one or more of the serum samples from all but one of the patients. igm antibodies were not detected in samples obtained from 11 of the patients after the 5th week of illness. absorption of sera for igg with staphylococcus aureus increased the sensitivity of the igm antibody test. it is concluded t ...1976188117
the clinical features of infantile gastroenteritis due to rotavirus.the symptoms of 100 hospitalised cases of rotavirus infantile gastroenteritis are described. most patients presented with high fever between the 2nd and 5th day, having started with diarrhoea or vomiting or both. 42% of the infants had upper respiratory tract symptoms. severe electrolyte disturbance did not occur, although there was a suggestion of a correlation between the higher blood ureas and the number of rotavirus particles in the stools. the mean duration of illness of uncomplicated cases ...1976188118
rotavirus-associated gastroenteritis in two adults probably caused by virus reinfection.rotavirus infection was diagnosed by virus detection and by serological methods in 2 women with acute gastroenteritis, aged 22 and 29 years, respectively. both patients had been in close contact with children with rotavirus gastroenteritis. rotavirus-specific antibodies were detected in serum specimens obtained prior to the illness in one of the patients, and the serological response in both patients suggested a reinfection with rotavirus as cause of the disease.1976188119
rotavirus associated with acute gastroenteritis in adults. 197673889
letter: child-mother transmission of rotavirus. 197654622
letter: rotavirus growth in bovine monolayers. 197656573
is lactase the receptor and uncoating enzyme for infantile enteritis (rota) viruses?rotaviruses are now regarded as important causes of diarrhoea in man, cattle, pigs, mice, and possibly other animals. characteristically, disease occurs in newborn and young animals, and infection seems limited to the differentiated gut epithelial cells. the major surface polypeptide of the calf scours rotavirus is glycosylated, and highly purified beta-galactosidase (lactase) interacts with the virus in vitro causing removal of the outer shell of the capsid (uncoating). it is suggested that lac ...197659021
haemagglutinin from rotavirus. 197662238
human rotavirus in young dogs. 1976190523
rotavirus infections in a maternity unit.between may and august 1975, rotaviruses were detected in the stools of 76 out of 174 (44%) newborn babies in the maternity unit at this hospital. infection occurred less frequently in breast-fed than in bottle-fed babies (p less than 0.001). however, only 7 out of 76 (8%) babies who excreted rotaviruses had symptoms and these were mild. complement fixation tests did not show any apparent difference in the antibody titres or serological responses between mothers of rotavirus positive or negative ...1976189702
probable role of viruses in calfhood diseases.bovine enteroviruses, bovine viral diarrhea virus, rotavirus (formerly called reovirus-like agent), coronavirus-like agent, bovine adenovirus, and bovine parainfluenza-3 virus have been isolated from calves suffering from neonatal disease. the experimental disease produced by these viruses is not necessarily severe or fatal, but under farm and ranch conditions, each probably serves as an added to mortality from neonatal disease. after initial losses following the introduction of a virus into a h ...1977190279
rotavirus and winter gastro-enteritis in white south african a microbiological investigation of winter gastro-enteritis in 23 white children rotaviruses were found in 14 (61%) and parvovirus-like particles in 1 (4%). bacteriological examination of stools from 11 of these patients yielded one isolate of salmonella eastbourne, but no enterotoxigenic or invasive bacteria were found. rotavirus appears to be the main aetiological agent of acute winter gastro-enteritis in white infants.1977204064
a study of the prevalence of rotavirus infection in children with gastroenteritis admitted to an infectious diseases a 12 month survey of infants and children with gastroenteritis admitted to fairfield hospital, melbourne, rotavirus was found in approximately 42% of patients. this virus was detected more often during the winter months, particularly in children aged between 12 months and 3 years. detection of rotavirus by electron microscopy was found to be more sensitive than by counterimmunoelectrophoresis. routine bacterial and viral studies revealed that bacterial pathogens and common enteric viruses wer ...1977204738
diarrhea with rotaviruses in a pediatric surgery in brussels. 1977205070
rotavirus isolation and cultivation in the presence of trypsin.rotaviruses are generally difficult to isolate and culture in vitro; therefore, virus isolation has not been used as a method of diagnosing this group of agents. the present report describes a simple procedure for isolating bovine rotaviruses directly from feces after pretreatment of fecal samples with trypsin. this procedure resulted in virus isolation from five of five samples that contained virus particles, as demonstrated by electron microscopy, and four of seven samples where virus particle ...1977201663
astrovirus associated gastroenteritis in a children's ward.during an outbreak of gastroenteritis in a paediatric ward astroviruses were found in faeces from 17 to 27 symptomatic children and from four of 14 members of the staff with diarrhoea. no viruses were found in 10 asymptomatic children. fourteen of the 21 astrovirus excretors were free of any recognised pathogens, but in the other seven, rotaviruses or pathogenic bacteria were also present. serological evidence of astrovirus infection was obtained in five adults and two children.1977201670
[rotavirus infections]. 1977201913
[duovirus (rotavirus) in belém, state of para, brazil (preliminary report)]. 1977202009
[acute gastroenteritis caused by rotavirus. a summary of danish studies]. 1977202050
[clinical significance of rotavirus infections in children with acute diarrhea. a preliminary study]. 1977202051
[acute diarrhea in childhood--new aspects]. 1977202052
rotavirus infection in lambs: pathogenesis and pathology.experimental lamb rotavirus infections were studied by immunofluorescence, histopathology and electron microscopy of tissues from infected gnotobiotic lambs killed at intervals from the incubation period to recovery. the rotavirus was demonstrated by immunofluorescence only in epithelial cells of villi in the small and large intestine, and virus antigen was most abundant during the incubation period. an increased enterocyte turnover rate was suggested by the rapid movement of virus-infected cell ...1977202226
[occurrence of rota- and coronavirus as a cause of neonatal calf diarrhea in the federal republic of germany]. 1977202239
detection of rotavirus in experimentally infected piglets.scouring and vomiting was induced in piglets by experimental infection with a field strain of rotavirus. virus or viral antigen was detected in the small intestine by the fluorescent antibody technique and virus could also be demonstrated in infected tissue culture cells by immuno-fluorescence. progeny particles in the epithelial layer of the small intestine were identified by electron microscopy. three morphological types could be distinguished, often associated with electron-dense inclusion, a ...1977190658
an extensive rotavirus outbreak in aboriginal infants in central australia.faecal specimens were obtained from 92 aboriginal infants admitted to alice springs hospital during an extensive outbreak of gastroenteritis in the surrounding area in july, 1976. specimens were examined for potentially pathogenic viruses, bacteria and parasites. rotavirus proved to be by far the most common potential pathogen, being detected in 54% of specimens.1977191738
[infantile diarrhea and rotavirus (author's transl)].the acute and convalescent sera of 328 children with acute enteritis were checked with the bovine diarrhea virus as antigen in complement fixation test. 13% had a seroconversion. some cases were associated with skin rashes or signs of respiratory infection.1977191779
transmission of rotavirus gastroenteritis from children to a monkey.a pooled suspension of rotavirus was prepared from the stools of eight children with acute non-bacterial gastroenteritis. the suspension was infused into the duodenum and stomach of an infant monkey (nemestrina macaque). biopsy samples of duodenal mucosa were taken at several intervals after inoculation, examined by light and electron microscopy, and assayed for lysosomal activity. virus-like particles were seen within and around microvilli and intracellularly within vesicles as early as 20 minu ...1977192637
rotavirus epidemic in adults. 1977193006
human rotavirus in lambs: infection and passive protection.a human stool filtrate containing rotavirus which was administered orally to gnotobiotic lambs caused diarrhea, virus excretion, development of antibodies to rotavirus, and pathological changes in the villi of the small intestine. thus, lambs may serve as experimental animals for the study of human rotavirus infections. this model system was used to study passive protection. human immunoglobulin g (igg) containing antibody to rotavirus was fed to lambs 24 to 78 h after birth, and the lambs were ...1977194837
enteropathogens associated with pediatric diarrhea in mexico city.enteropathogens were investigated as possible agents in pediatric diarrhea occurring in mexico city during the summer of 1975. pathogens were identified in 47 (76%) of 62 cases. rotavirus particles were detected in 16 cases. enterotoxigenic escherichia coli was detected in 29 cases; 11 were positive for heat-labile enterotoxin and 18 were positive for only the heat-stable form of enterotoxin. multiple pathogens were found simultaneously in 15 (24%) of the study population. this study indicates t ...1977195030
[gastroenteritis due to rotavirus and spreading of the infection through the hospital]. 1977195230
viruses associated with acute gastroenteritis in young children.conventional virologic investigations generally failed to implicate viruses as a cause of acute gastroenteritis in young children. when negative-contrast stain electron microscopy was used for the examination of stool samples from affected patients, several candidate etiologic agents were discovered. rotavirus (otherwise known as orbivirus, reo-like agent, and infantile gastroenteritis virus) emerged as an important causative agent. in a considerable number of patients, however, no causative age ...1977195461
intestinal damage in rotavirus and adenovirus gastroenteritis assessed by d-xylose malabsorption.the absorption of d-xylose infused into the duodenum was assessed in infants with acute gastroenteritis. 1-hour blood-xylose levels were low in 6 patients found to harbour rotavirus in the small intestinal aspirate. normal levels (greater than 1-26 mmol/l) were obtained in the absence of virus particles in the small intestine in a further 6 patients: in 3 of these adenovirus or rotavirus was recovered from the stools. three patients with adenovirus in the small intestinal juice and ill with acut ...1977195541
quantitation of antibody to non-hemagglutinating viruses by single radial hemolysis: serological test for human coronaviruses.a single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. with the human coronaviruses as models, this test utilizes the binding properties of the chromic cation to attach viruses to glutaraldehyde-treated sheep erythrocytes. the most satisfactory system consisted of stabilizing washed sheep erythrocytes with 0.0073% glutaraldehyde for 15 min at 23 degrees c, binding a high concentration of virus to a 25% erythrocyte suspension with 0.001 ...1977195976
solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. the test involved ethanol fixation of microcultures of bovine rotavirus-infected bsc-1 cells and reacetion with bovine antirotavirus serum, followed by 125i-labeled rabbit anti-bovine immunoglobulin g. the technique was shown to be virus specific and highly sensitive. the fixed microcultures could be stored at 4 degrees c for at least 2 months without affecting the sensitivity of the test. th ...1977195982
enterotoxigenic escherichia coli diarrhea of travelers: a prospective study of american peace corps' diarrhea was studied prospectively in a group of 39 american peace corps volunteers (pcvs) during their first five weeks in kenya. twenty-seven developed diarrheal disease and 12 remained well. multiple episodes were documented in 11 of the symptomatic volunteers. enterotoxigenic escherichia coli of many serotypes producing heat-labile and/or heat-stable enterotoxin were isolated from 17 of the 27 volunteers with diarrhea and from 1 of the 12 well volunteers. the enterotoxigenic e. co ...1977196131
the action of disinfectants on lamb rotavirus. 1977197685
antigenic relationship between human and bovine rotaviruses as determined by neutralization, immune adherence hemagglutination, and complement fixation tests.guinea pig antiserum to bovine rotavirus does not neutralize human rotavirus. bovine and human rotaviruses were, however, extensively cross-reactive when examined by complement fixation and immune adherence hemagglutination tests with antiserum to either virus. the immune adherence hemagglutination test was 16- to 32-fold more sensitive than the complement fixation test in detecting rotavirus.1977198371
pathological changes in the small intestine of neonatal pigs infected with a pig reovirus-like agent (rotavirus). 1977198438
further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses.polyacrylamide gel electrophoretic analysis of purified preparation of the simian rotavirus sa-11 indicated eight polypeptide components that migrated in a manner remarkably similar to those of the previously characterized human and calf rotaviruses. analyses of preparations of single-shelled and double-shelled particles of human, calf, and simian an rotaviruses have also permitted assignment of the polypeptides to the inner or outer shells. the major components of the outer shells of each virus ...1977198589
human rotavirus and intussusception. 1977198658
[application of enzyme-linked immunosorbent assay (elisa) to the detection of calf rotavirus and rotavirus antibodies (author's transl)]. 1977199102
[rotavirus in children with severe malnutrition].the study comprised 19 children with severe protein-calorie malnutrition admitted to hospital nacional de niños. with one exception, all of the patients complained of diarrhea at the examination. samples of stools were collected for bacteriologic, parasitologic and virologic studies. rotavirus was shown in 4 cases and adenovirus in another 4. enteropathogenic escherichia coli, shigella and salmonella were isolated in 9 different children. it was possible to demonstrate at least one potentially p ...1977199211
rotavirus gastroenteritis. 1977199315
virions associated with acute gastroenteritis in vancouver, 1976.virions were identified by electron microscopic observation of unconcentrated extracts of feces obtained from 55 (33%) of 167 children aged 3 weeks to 5 years who were hospitalized with acute gastroenteritis at children's hospital, vancouver between january and december 1976. morphologic types included 30 rotavirus, 15 adenovirus, 5 astrovirus and 5 picornavirus-like particles.1977199339
review of enterovirus studies in east africa. 1977199414
filter paper solid-phase radioimmunoassay for human rotavirus surface immunoglobulins.a filter paper solid-phase radioimmunoassay has been developed. filter paper disks adsorbed a large amount of rotavirus and serum globulin and gave small mean variation of coating and low background binding. the rotavirus isolated from stools from infants with acute enteritis 1, 3, and 4 days after onset of symptoms was shown to be already covered with immunoglobulin g (igg), iga, and igm antibodies by this radioimmunoassay, by immunoelectrophoresis, and by immune electron microscopy. the immuno ...1977199613
human rotavirus enteritis induced in conventional piglets. intestinal structure and better understand the pathogenesis of infantile viral gastroenteritis, we studied na+ and cl- fluxes in vitro in short-circuited jejunal epithelium from 8-10-day-old piglets after infection with a standard dose of human rotavirus given via nasogastric tube. 11 infected piglets, all of whom became ill, were compared with 9 uninfected, healthy litter-mates. when killed 72 h after infection, intestinal villi were shorter and crypts deeper (p less than 0.025) in duodenum, upper jejunum, and mid-s ...1977199622
separation and infectivity of two particle types of human rotavirus.two morphological types of human rotavirus particles were separated in caesium chloride density gradients. the particles of higher density ('incomplete' particles without an outer capsid layer) banded at a density of 1-38 g/ml, while the less dense ('complete' particles with an outer as well as an inner capsid layer) banded at a density of 1-36 g/ml. some particles were found with an incomplete outer layer of capsomeres. the particle/infectivity ratio for tissue cultures of the fraction containi ...1977199702
antiviral activity of ribavirin in rotavirus gastroenteritis of mice.ribavirin was inactive against the rotavirus of murine gastroenteritis; this may be due to the presence of guanosine inhibitors in the gut.1977200170
enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in enzyme-linked immunosorbent assay for the diagnosis of rotavirus infection in calves is described. the assay was more efficient for the detection of rotavirus antigens in calf feces than were electron microscopy and immunoelectroosmorphoresis.1977200635
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