PMID(sorted ascending)
viruses associated with acute gastroenteritis in young children.conventional virologic investigations generally failed to implicate viruses as a cause of acute gastroenteritis in young children. when negative-contrast stain electron microscopy was used for the examination of stool samples from affected patients, several candidate etiologic agents were discovered. rotavirus (otherwise known as orbivirus, reo-like agent, and infantile gastroenteritis virus) emerged as an important causative agent. in a considerable number of patients, however, no causative age ...1977195461
intestinal damage in rotavirus and adenovirus gastroenteritis assessed by d-xylose malabsorption.the absorption of d-xylose infused into the duodenum was assessed in infants with acute gastroenteritis. 1-hour blood-xylose levels were low in 6 patients found to harbour rotavirus in the small intestinal aspirate. normal levels (greater than 1-26 mmol/l) were obtained in the absence of virus particles in the small intestine in a further 6 patients: in 3 of these adenovirus or rotavirus was recovered from the stools. three patients with adenovirus in the small intestinal juice and ill with acut ...1977195541
quantitation of antibody to non-hemagglutinating viruses by single radial hemolysis: serological test for human coronaviruses.a single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. with the human coronaviruses as models, this test utilizes the binding properties of the chromic cation to attach viruses to glutaraldehyde-treated sheep erythrocytes. the most satisfactory system consisted of stabilizing washed sheep erythrocytes with 0.0073% glutaraldehyde for 15 min at 23 degrees c, binding a high concentration of virus to a 25% erythrocyte suspension with 0.001 ...1977195976
solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. the test involved ethanol fixation of microcultures of bovine rotavirus-infected bsc-1 cells and reacetion with bovine antirotavirus serum, followed by 125i-labeled rabbit anti-bovine immunoglobulin g. the technique was shown to be virus specific and highly sensitive. the fixed microcultures could be stored at 4 degrees c for at least 2 months without affecting the sensitivity of the test. th ...1977195982
enterotoxigenic escherichia coli diarrhea of travelers: a prospective study of american peace corps' diarrhea was studied prospectively in a group of 39 american peace corps volunteers (pcvs) during their first five weeks in kenya. twenty-seven developed diarrheal disease and 12 remained well. multiple episodes were documented in 11 of the symptomatic volunteers. enterotoxigenic escherichia coli of many serotypes producing heat-labile and/or heat-stable enterotoxin were isolated from 17 of the 27 volunteers with diarrhea and from 1 of the 12 well volunteers. the enterotoxigenic e. co ...1977196131
the action of disinfectants on lamb rotavirus. 1977197685
antigenic relationship between human and bovine rotaviruses as determined by neutralization, immune adherence hemagglutination, and complement fixation tests.guinea pig antiserum to bovine rotavirus does not neutralize human rotavirus. bovine and human rotaviruses were, however, extensively cross-reactive when examined by complement fixation and immune adherence hemagglutination tests with antiserum to either virus. the immune adherence hemagglutination test was 16- to 32-fold more sensitive than the complement fixation test in detecting rotavirus.1977198371
pathological changes in the small intestine of neonatal pigs infected with a pig reovirus-like agent (rotavirus). 1977198438
further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses.polyacrylamide gel electrophoretic analysis of purified preparation of the simian rotavirus sa-11 indicated eight polypeptide components that migrated in a manner remarkably similar to those of the previously characterized human and calf rotaviruses. analyses of preparations of single-shelled and double-shelled particles of human, calf, and simian an rotaviruses have also permitted assignment of the polypeptides to the inner or outer shells. the major components of the outer shells of each virus ...1977198589
human rotavirus and intussusception. 1977198658
[application of enzyme-linked immunosorbent assay (elisa) to the detection of calf rotavirus and rotavirus antibodies (author's transl)]. 1977199102
[rotavirus in children with severe malnutrition].the study comprised 19 children with severe protein-calorie malnutrition admitted to hospital nacional de niños. with one exception, all of the patients complained of diarrhea at the examination. samples of stools were collected for bacteriologic, parasitologic and virologic studies. rotavirus was shown in 4 cases and adenovirus in another 4. enteropathogenic escherichia coli, shigella and salmonella were isolated in 9 different children. it was possible to demonstrate at least one potentially p ...1977199211
rotavirus gastroenteritis. 1977199315
virions associated with acute gastroenteritis in vancouver, 1976.virions were identified by electron microscopic observation of unconcentrated extracts of feces obtained from 55 (33%) of 167 children aged 3 weeks to 5 years who were hospitalized with acute gastroenteritis at children's hospital, vancouver between january and december 1976. morphologic types included 30 rotavirus, 15 adenovirus, 5 astrovirus and 5 picornavirus-like particles.1977199339
review of enterovirus studies in east africa. 1977199414
filter paper solid-phase radioimmunoassay for human rotavirus surface immunoglobulins.a filter paper solid-phase radioimmunoassay has been developed. filter paper disks adsorbed a large amount of rotavirus and serum globulin and gave small mean variation of coating and low background binding. the rotavirus isolated from stools from infants with acute enteritis 1, 3, and 4 days after onset of symptoms was shown to be already covered with immunoglobulin g (igg), iga, and igm antibodies by this radioimmunoassay, by immunoelectrophoresis, and by immune electron microscopy. the immuno ...1977199613
human rotavirus enteritis induced in conventional piglets. intestinal structure and better understand the pathogenesis of infantile viral gastroenteritis, we studied na+ and cl- fluxes in vitro in short-circuited jejunal epithelium from 8-10-day-old piglets after infection with a standard dose of human rotavirus given via nasogastric tube. 11 infected piglets, all of whom became ill, were compared with 9 uninfected, healthy litter-mates. when killed 72 h after infection, intestinal villi were shorter and crypts deeper (p less than 0.025) in duodenum, upper jejunum, and mid-s ...1977199622
separation and infectivity of two particle types of human rotavirus.two morphological types of human rotavirus particles were separated in caesium chloride density gradients. the particles of higher density ('incomplete' particles without an outer capsid layer) banded at a density of 1-38 g/ml, while the less dense ('complete' particles with an outer as well as an inner capsid layer) banded at a density of 1-36 g/ml. some particles were found with an incomplete outer layer of capsomeres. the particle/infectivity ratio for tissue cultures of the fraction containi ...1977199702
antiviral activity of ribavirin in rotavirus gastroenteritis of mice.ribavirin was inactive against the rotavirus of murine gastroenteritis; this may be due to the presence of guanosine inhibitors in the gut.1977200170
enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in enzyme-linked immunosorbent assay for the diagnosis of rotavirus infection in calves is described. the assay was more efficient for the detection of rotavirus antigens in calf feces than were electron microscopy and immunoelectroosmorphoresis.1977200635
distribution and titres of rotavirus antibodies in different age groups.three hundred and fifty-seven sera selected at random from hospital patients of all ages were examined for rotavirus antibodies using indirect immunofluorescence (fa) and complement fixation levels (cft). three hundred and fourteen of these were also tested for neutralizing antibodies to human rotavirus. sera from patients admitted with a diagnosis of acute gastroenteritis were excluded from this survey. fa antibodies were found in newborn infants but fell to undetectable titres at 3 months. the ...1977200676
observations on the morphology of two rotaviruses.the negative staining technique was used to study the morphology of two rotaviruses, the epizootic diarrizootic diarrhoea of infant mice (edim) and the simian virus sa ii. it is proposed that the inner capsid of the virion has icosahedral symmetry and consists of 180 morphological units arranged in an open lattice formation with the 12 spaces at the apices being surrounded by 5 capsomeres and the other 80 spaces each surrounded by six capsomeres. the outer capsid of the virion consists of a hone ...1977200716
cell culture propagation of porcine rotavirus (reovirus-like agent).two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ...1977201198
rotavirus neutralisation by human milk. 1977201336
hemagglutination and hemagglutination-inhibition studies with a strain of nebraska calf diarrhea virus (bovine rotavirus).a hemagglutinin has been prepared from nebraska calf diarrhea virus (ncdv) propagated in bs-c-1 cell line. after cesium chloride centrifugation, the hemagglutinin of the bovine rotavirus was found to be associated with intact virions (density 1.355 g/ml) but not with virions lacking an outer capsid layer (density 1.375 g/ml). in hemagglutination-inhibition (hai) tests, the hemagglutinin reacted specifically with ncdv serum, and hai seroconversions were detected in some sera tested. cross-reactio ...1978201585
rotavirus isolation and cultivation in the presence of trypsin.rotaviruses are generally difficult to isolate and culture in vitro; therefore, virus isolation has not been used as a method of diagnosing this group of agents. the present report describes a simple procedure for isolating bovine rotaviruses directly from feces after pretreatment of fecal samples with trypsin. this procedure resulted in virus isolation from five of five samples that contained virus particles, as demonstrated by electron microscopy, and four of seven samples where virus particle ...1977201663
astrovirus associated gastroenteritis in a children's ward.during an outbreak of gastroenteritis in a paediatric ward astroviruses were found in faeces from 17 to 27 symptomatic children and from four of 14 members of the staff with diarrhoea. no viruses were found in 10 asymptomatic children. fourteen of the 21 astrovirus excretors were free of any recognised pathogens, but in the other seven, rotaviruses or pathogenic bacteria were also present. serological evidence of astrovirus infection was obtained in five adults and two children.1977201670
[rotavirus infections]. 1977201913
[duovirus (rotavirus) in belém, state of para, brazil (preliminary report)]. 1977202009
[acute gastroenteritis caused by rotavirus. a summary of danish studies]. 1977202050
[clinical significance of rotavirus infections in children with acute diarrhea. a preliminary study]. 1977202051
[acute diarrhea in childhood--new aspects]. 1977202052
rotavirus infection in lambs: pathogenesis and pathology.experimental lamb rotavirus infections were studied by immunofluorescence, histopathology and electron microscopy of tissues from infected gnotobiotic lambs killed at intervals from the incubation period to recovery. the rotavirus was demonstrated by immunofluorescence only in epithelial cells of villi in the small and large intestine, and virus antigen was most abundant during the incubation period. an increased enterocyte turnover rate was suggested by the rapid movement of virus-infected cell ...1977202226
[occurrence of rota- and coronavirus as a cause of neonatal calf diarrhea in the federal republic of germany]. 1977202239
[epidemiological aspects of gastroenteritis dut to rotavirus].during december 1976 to january-march 1977, 13/53 children less than 5 years of age were admitted with acute enteritis caused by rotaviruses to the hospital del niño, dif. there is already convincing evidence that this new virus will prove to be the most important etiological agent of sporadic acute enteritis in young children.1978202294
a simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane compounds in cell culture harvests known as membrane-coating components (mcc) prevent virus adsorption to membrane filters. blending cell culture harvests with fluorocarbon removed the mcc and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. subsequent elution with high ph buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.1978202572
the role of breast-feeding in the prevention of rotavirus infection.breast-fed infants are less susceptible to gastroenteritis than bottle-fed infants. antibodies against rotavirus, the major pathogen of infantile gastroenteritis, were sought in human sera, colostrum and milk specimens by immunofluorescence. an experimental murine-rotavirus model was established by infecting the second litters of dams 4 weeks after infecting their first litters. antibodies were absent from human and murine colostrum and milk specimens despite being present in virtually all sera, ...1978202710
rearing regimen producing piglet diarrhea (rotavirus) and its relevance to acute infantile diarrhea.piglets were weaned when 1 day old and thus were denied further access to the antibodies supplied by their dam's milk. they were placed in a nursery in which contamination by the ubiquitous rotavirus steadily increased with continuous use causing a progressive increase in the incidence of vomiting, diarrhea, and death among the piglets. a similar syndrome involving an antigenically related rotavirus and analogous management practices occurs in human infants.1978203032
[existence of 2 types of rotavirus associated with acute gastroenteritis in children].rotaviruses found in the stools of different infants, hospitalized in the hospital del niño del dif, may be grouped into at least two types according to their rna composition. the viral rna composition was analyzed by electrophoresis in agarose gels. the different types can be distinguished by the mobility of the second larger of the 11 segments that make up the viral rna.1978203294
[diagnosis of rotavirus using viral rna electrophoresis].rotavirus has emerged as a major agent of acute enteritis of infants. a rapid and simple technique has been developed for its detection, based on the visualization of the viral rna electrophoretic pattern. this technique takes advantage of the large amount of rotavirus present in the stools and the high sensitivity for detection of rna obtained with ethidium bromide.1978203295
mechanisms in the pathogenesis of diarrhea: a review. 1978203563
rotavirus as a cause of diarrhea in pigs.a rotavirus (reovirus-like agent) was associated with diarrheal diseases occurring in 1- to 4-week-old suckling pigs in 8 herds and in weaned pigs in 2 herds. transmissible gastroenteritis virus was also detected in 2 of these herds, as was enteropathogenic escherichia coli in 5 herds. morbidity was generally greater than 80% in pigs of the affected age group within these herds, and mortality from diarrhea ranged from 7 to 20%. the disease due to rotavirus in suckling pigs appeared similar to th ...1978203565
rotavirus and winter gastro-enteritis in white south african a microbiological investigation of winter gastro-enteritis in 23 white children rotaviruses were found in 14 (61%) and parvovirus-like particles in 1 (4%). bacteriological examination of stools from 11 of these patients yielded one isolate of salmonella eastbourne, but no enterotoxigenic or invasive bacteria were found. rotavirus appears to be the main aetiological agent of acute winter gastro-enteritis in white infants.1977204064
pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs.porcine rotavirus was shown to infect gnotobiotic pigs and induce an acute enteric disease clinically characterized by diarrhea, anorexia, depression, and occasional vomition. onset of clinical signs correlated closely with the appearance of lesions within the small intestinal mucosa, and recovery from infection was associated with the regeneration of normal, functional villous epithelium. villous atrophy, especially in the caudal two-thirds of the small intestine, was the consistent lesion obse ...1978204232
calf rotavirus: detection of outer capsid glycoproteins by lectins. 1978204517
epidemic gastroenteritis in children associated with rotavirus infection. 1977204575
routine diagnosis of human rotaviruses in stools.electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.1978204665
rotavirus in travelers' diarrhea: study of an adult student population in mexico.the role of rotavirus in adult diarrhea was evaluated in 165 students attending a mexican university. students were divided into three groups: newly arrived summer students from the united sttes, regular students from the united states, and mexican and venezuelan students. ninety-one students with diarrhea and 74 corresponding, matched, asymptomatic control students were included in the study. the frequency of rotavirus in stools was determined by electron microscopy with use of the pseudoreplic ...1978204710
a study of the prevalence of rotavirus infection in children with gastroenteritis admitted to an infectious diseases a 12 month survey of infants and children with gastroenteritis admitted to fairfield hospital, melbourne, rotavirus was found in approximately 42% of patients. this virus was detected more often during the winter months, particularly in children aged between 12 months and 3 years. detection of rotavirus by electron microscopy was found to be more sensitive than by counterimmunoelectrophoresis. routine bacterial and viral studies revealed that bacterial pathogens and common enteric viruses wer ...1977204738
central nervous system involvement in patients with rotavirus gastroenteritis.two children with rotavirus gastroenteritis are presented. the first case developed a fatal reye's syndrome and the other one encephalitis with slow recovery. the rotavirus diagnosis was made in both cases by electron microscopy and a significant rise in antibody titres to nebraska calf diarrhea virus was seen in one of the two patients.1978204984
[rotaviruses as pathogens for calf diarrhea: prevalence of complement fixing antibodies in fattening calves]. 1978204995
trypsin and bovine rotavirus replication. 1978205033
diarrhea with rotaviruses in a pediatric surgery in brussels. 1977205070
comparison between adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs.adsorption of poliovirus and rotavirus by aluminum hydroxide and activated sludge flocs was studied. both aluminum hydroxide and activated sludge flocs adsorbed greater amounts of poliovirus than rotavirus. aluminum hydroxide flocs reduced the titer of poliovirus in tap water by 3 log10, but they only reduced the titer of a simian rotovirus (sa-11) in tap water by 1 log10 or less and did not noticeably reduce the number of human rotavirus particles present in a dilute stool suspension. activated ...1978205173
comparison of rotavirus strains by hemagglutination inhibition.rotaviruses have been shown to be of importance as aetiologic agents of gastroenteritis in infants and in domestic animals of several species. hemagglutinins were prepared from two canadian isolates of bovine rotavirus and from one isolate of a simian rotavirus. a united kingdon isolate of bovine rotavirus was shown not to possess hemagglutinating activity, indicating a strain difference between a canadian and united kingdom bovine rotavirus. in hemagglutination-inhibition (hai) tests a rabbit h ...1978205329
a study of etiologic agents in infantile gastroenteritis. 1978205524
a simplified method for detecting fluorescent antibody to rotavirus: its application to sero-epidemiology. 1978205558
the immunoprophylaxis of of rotavirus infections in lambs.the effect of colostrum or serum feeding on subsequent rotavirus infections was investigated in neonatal gnotobiotic lambs. immunity after feeding colostrum did not depend on absorption of passively acquired antibody into the circulation. protection against clinical disease depended on the volume of colostrum ingested. the protection afforded by feeding serum was specific, since serum free of rotavirus antibody failed to confer protection. immune serum fed at a rate of 2.5 ml per kg twice daily ...1978205988
multiple viruses present in the faeces of a scouring calf. 1978205989
fatal rotavirus gastroenteritis: an analysis of 21 cases.during the period of may 1972 to march 1977, twenty-one fatal cases of rotavirus acute gastroenteritis were recorded in the city of toronto. the mean age of these subjects was approximately 1 year. boys outnumbered girls by 12 to 9. death occurred within three days of onset of symptoms in all cases. sixteen of the subjects were profoundly dehydrated and had sodium levels (serum or vitreous humor) in excess of 150 meq/liter. in 11 subjects, sodium values were greater than 160 meq/liter. although ...1978206132
lapine rotavirus: preliminary studies on epizoology and transmission.antibody to rotavirus was found in sera of domestic rabbits, wild cottontail rabbits and snowshoe hares. eight out of 187 stools from a rabbitry contained rotavirus, though no clinical symptoms were apparent. the virus extracted from one such stool infected a five week old seronegative rabbit and led to distention and congestion of the colon, excretion of virus in the stool and a serological response to virus. human and simian rotaviruses did not replicate in the rabbits.1978206325
characteristics of neonatal calf diarrhea virus ribonucleic acid.the nucleic acids of neonatal calf diarrhea virus were characterized by isopycnic centrifugation in cs2so4, electron microscopy, ultraviolet absorbance temperature profiles and polyacrylamide gel electrophoresis. these studies indicated that the neonatal calf diarrhea virus genome consists of 11 segments of double stranded rna with a total molecular weight of 10.75 million daltons.1978206326
diarrhea in newborn cynomologus monkeys infected with human rotavirus.of six newborn cynomolgus monkeys (macaca fascicularis) naturally delivered and normally nursed five developed diarrhea after oral administration of human rotavirus. virus excretion was observed in the stool of four animals. this virus was transmitted to four out of six other monkeys causing diarrhea in only one animal.1978206515
hemagglutination by simian rotavirus.a simian rotavirus (sa-11) was shown to hemagglutinate human group "o" and guinea pig erythrocytes. the hemagglutinin appeared to be associated with the outer capsid of the sa-11 virus and was inhibited by specific hyperimmune anti-sa-11 guinea pig serum.1978206575
morphogenesis of porcine rotavirus in porcine kidney cell cultures and intestinal epithelial cells.the morphogenesis of porcine rotavirus was similar in vitro in porcine kidney (pk) cell cultures and in vivo in porcine epithelial cells as examined by electron microscopy. infected cells contained cytoplasmic, non-membrane-bound viroplasm and accumulations of virus particles within cisternae of the rough endoplasmic reticulum (rer). three types of virus particles were noted: double-shelled or complete particles which averaged 77 nm in diam.; single-shelled or naked particles which ranged from 5 ...1978206653
diarrhoea in pigs induced by rotavirus.electron microscope examination of the faeces of scouring pigs revealed virus particles which were morphologically indistinguishable from rotavirus (reo-like), a virus associated with diarrhoea in neonatal pigs (lecce, king & mock, 1976). this is the first record of the virus in the republic of south africa.1977206862
[pediatrics in 1977]. 1978206954
prevalence of antibodies to a rotavirus in black and white populations in south africa.a serological survey of antibodies to a rotavirus in 252 sera from black and white south africans was performed by means of an immunofluorescence test using cells infected with simian rotavirus, sa 11, as antigen. the results indicate widespread presence of this agent in south africa and also that the black population is exposed to a greater extent than the white population.1978206980
nosocomial rotavirus gastroenteritis in a neonatal nursery. 1978206997
intestinal damage in rotavirus infected calves assessed by d-xylose malabsorption. 1978207007
isolation of rotavirus from calves in the federal republic of germany. 1978207058
[rotavirus as a causative agent of infantile gastroenteritis. diagnosis and epidemiology (author's transl)].some years ago it was not possible in spite of intensive bacteriological and virological work on the etiology of acute infantile gastroenteritis to find any agent that may readily be linked to this disease. since five years now early sporadic reports about virus-like particles have proved worldwide to be correct and in the meantime have substantially been supported by new findings in that these particles are the main causative agent of infantile gastroenteritis. as part of our electron-microscop ...1978207634
human rotavirus and its antibody: their coexistence in feces of infants.rotavirus and its antibody were detected by paper disk solid-phase radioimmunoassay or electron microscopy in feces of infants and young children with acute diarrhea. the fecal specimens in which rotavirus was detectable often contained a high titer of antibodies, which were shown by radioimmunoassay to belong mainly to the immunoglobulin g class. rotavirus was rarely detected in the specimens containing immunoglobulin a antibodies. by dissociation tests carried out by radioimmunoassay, it was s ...1978207717
rna polymerase associated with human rotaviruses in diarrhea stools.rna polymerase activity was detected in six stools which were partially purified by high-speed centrifugation from infants with rotavirus gastroenteritis, but was not detected in five stools which were negative for rotavirus by counterimmunoelectrophoresis and radioimmunoassay. the polymerase activity was associated with the 1.38-g/ml rotavirus band after purification in a cscl gradient.1978207902
a survey of rotavirus associated with gastroenteritis in aboriginal children in western a year-long survey which was carried out between april, 1975, and march, 1976, faecal specimens from aboriginal and some non-aboriginal children who suffered from gastroenteritis were examined by electron microscopy for rotavirus, and also tested for bacteria and parasites. the children were under six years of age and came from all parts of western australia, except perth. rotaviruses, "astroviruses" and adenoviruses were detected as well as the usual potentially pathogenic bacteria and paras ...1978207954
enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species.rotaviruses cause gastroenteritis in man and a wide variety of animal species. they cross-react in many immunologic tests and have a similar appearance by electron microscopy, making differentiation among them difficult. rotaviruses derived from different host species were distinguished by postinfection serum blocking virus activity in an enzyme-linked immunosorbent assay (elisa). thirty-three rotavirus isolates from children living in three different parts of the world could not be differentiat ...1978208150
pathological changes in the small intestine of neonatal calves naturally infected with reo-like virus (rotavirus).nine calves, of which six had been challenged with an enteropathogenic strain of escherichia coli, were found to be naturally infected with rotavirus. rotavirus was recovered from the faeces of six calves and rotavirus antigen was detected in the intestinal mucosa of all calves. stunting and fusion of villi were seen principally in the proximal and middle small intestine, where rotavirus antigen was detected by immunofluorescence. typical lesions of enteric colibacillosis were found in the dista ...1978208222
comparison of human and animal rotavirus strains by gel electrophoresis of viral rna. 1978208274
rotavirus gastroenteritis. 1978208471
diagnosis of viral agents associated with neonatal calf diarrhea.during this study, 134 samples have been examined for the detection of the viruses associated with neonatal calf diarrhea. the presence of nebraska viruses (rotavirus and coronavirus) has been demonstrated by using the electron microscope and the fluorescent antibody techniques while the presence of other viruses has been detected by the observation of a cytopathic effect on monolayer cells of calf testis. the nebraska viruses have been demonstrated in 107 (80%) out of 134 field case specimens. ...1978208735
noncultivable viruses and neonatal diarrhea: fifteen-month survey in a newborn special care nursery.during a 15-month period of surveillance, diarrhea developed in 257 of 913 babies (28%) admitted within 2 hours of birth to a special care nursery in melbourne, australia. diarrhea was seasonal, affecting a maximum of 43% of babies admitted during one winter month (july) and a minimum of 13% of babies admitted during one summer month (december). diarrhea was no more frequent nor more severe in babies of very low birth weight or of very early gestational age. two noncultivable viruses were locate ...1978209058
oral hydration rotavirus diarrhoea: a double blind comparison of sucrose with glucose electrolyte solution.of 57 male children, aged 5 months to 2 1/2 years with rotavirus diarrhoea, 28 were given oral therapy with sucrose electrolyte solution and 29 were given glucose electrolyte solution in a randomised double-blind trial. all were rehydrated and remained so on oral therapy alone. these patients were compared with 44 children, also with rotavirus, who were treated only with intravenous hydration. the oral therapy and intravenous therapy groups did not differ clinically in the rate of rehydration or ...1978209263
effect of serum albumin on hemagglutination with nebraska calf diarrhea virus. 1978209292
[identification of rotavirus by counter-immunoelectrophoresis in cases of infantile diarrhea]. 1978209531
antibody to rotavirus in dogs and cats. 1978209605
epidemiological aspects of rotavirus infection in hospitalized venezuelan children with gastroenteritis.the prevalence of rotavirus infection in hospitalized venezuelan children with gastroenteritis was studied during the period november 1975 to december 1976. rotaviruses were the pathogens most frequently associated with gastroenteritis, being found in 121 of 293 (41.3%) patients and in only 3 of 66 (4.5%) controls. other viruses (adenoviruses, enteroviruses, and small icosahedrical viruses) were detected at a lower frequency both in cases and controls. rotaviruses were detected at a lower freque ...1978209696
rotavirus infections in adults in association with acute gastroenteritis.during an epidemic of acute gastroenteritis in helsinki, in march--may 1976, 18 out of 40 adult patients showed electron microscopic and/or serologic evidence for rotavirus infection. rotavirus was most frequently seen in the fecal suspensions from 2 to 6 days after the onset of the symptoms but persisted in one patient for as long as 10 days. an increase in the complement-fixing (cf) serum antibody titers against the related nebraska calf diarrhea virus (ncdv), or an initially high titer and su ...1978210251
serial studies of virus multiplication and intestinal damage in gnotobiotic piglets infected with rotavirus.a serial study of rotavirus infection in gnotobiotic piglets is described. they were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. there appeared to be two stages of recovery of the intestinal wall, the earlier stage involving non-immune mechanisms and the later invloving antibody.1978210280
[rotavirus infections]. 1978210442
studies on cross protection induced in calves by rotaviruses of calves, children and foals.inoculation at birth with a live attenuated strain of a bovine rotavirus isolated in the usa (scourvax-reo) induced protection in five gnotobiotic calves seven to 21 days later against a uk isolate of pathogenic bovine rotavirus. however, no protection was induced in three calves challenged three to five days after vaccination. there was a close antigenic relationship demonstrated between the two bovine rotavirus isolates. in contrast only one of three gnotobiotic calves inoculated with foal rot ...1978210557
comparison of human rotavirus disease in tropical and temperate settings.human rotaviruses (hrv) are a common cause of acute nonbacterial gastroenteritis in pediatric patients. a prospective study of hrv disease in a temperate (dallas) and a tropical (san jose, costa rica) setting demonstrated differences in seasonal distribution. in both locales, hrv accounted for 50% to 60% of acute nonbacterial gastroenteritis episodes from december through february; this period corresponded to the cooler months of winter in dallas and to the dry season in san jose. during the res ...1978210656
diarrhoea in piglets inoculated with rotavirus.a rotavirus isolated from a field outbreak of diarrhoea in artificially reared piglets was purified, filtered and administered orally to gnotobiotic and conventional piglets. four successive passages of the virus in gnotobiotic piglets produced severe diarrhoea within 20 to 24 hours of administration. the diarrhoea lasted several days causing dehydration, emaciation, loss of body weight and some deaths. virus was demonstrated in the faeces of the infected piglets by electron microscopy. conventi ...1978210755
[role of bacteria and viruses in the etiology of acute gastroenteritis in children]. 1978211023
panel report on the colloquium on selected diarrheal diseases of the young. 1978211109
the effect of trypsin on the growth of has been found that 1000-fold more bovine rotavirus is obtained when trypsin is incorporated in the maintenance medium and allowed to remain throughout the growth cycle. this holds true for primary calf kidney (ck) cells and also for several continuous and semi-continuous cell lines. in the presence of trypsin it has been possible to pass the virus serially on continuous cell lines seven times. concentrations of 1 to 10 microgram/ml of trypsin are found to be effective. preliminary results su ...1978211180
prospective study of enteropathogens in children with diarrhea in houston and mexico.during a 22-month period, 595 children with diarrhea and 210 age-matched controls attending clinics in houston (367 children) and mexico (438) were prospectively evaluated for enteric pathogens. enteropathogens associated with disease were shigella (18%), rotavirus (14%), salmonella (9%), toxigenic escherichia coli (6%), and others (12%), including 14 proteus isolates that caused rounding of adrenal cells. enteropathogens were isolated from a greater (p less than 0.001) number of children with d ...1978211215
a clinical study of rotavirus gastroenteritis. 1978211217
rotavirus (reovirus-like) infection of neonatal ruminants in a zoo outbreak of a pneumoenteric disease occurred in neonates in a zoo nursery. four of seven affected animals died. rotaviruses were observed in the feces of an affected 4-day old impala (aepyceros melampus), a thomson's gazelle (gazella thomsonii) and an addax (addax nasomaculatus). encapsulated escherichia coli also were isolated from the feces. the recovered rotaviruses was antigenically related to bovine rotavirus. a bovine rotavirus vaccine was given orally and no adverse effects were noted.1978211273
[viruses and children's diarrhoea]. 1978211647
epizootiology of bovine rotavirus infection.published information on rotaviruses as pathogens, the source of virus infection and the method of transmission of infection under normal conditions are reviewed. the antigenic differences between rotavirus isolates from children, calves, pigs, foals and mice are discussed. bovine rotaviruses isolated in the usa and the uk were shown to be closely related antigenically and the us vaccine strain protected calves from challenge with the uk rotavirus. nineteen normally reared calves, with 20 or mor ...1978211696
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