Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification of the cell binding domain in nipah virus g glycoprotein using a phage display system. | nipah virus (niv) is a highly pathogenic zoonotic paramyxovirus with unusual broad host tropism and is designated as a category c pathogen by the u.s. national institute of allergy and infectious diseases. niv infection is initiated after binding of the viral g glycoprotein to the host cell receptor. the aim of this study was to map the niv g glycoprotein cell binding domain using a phage display system. the niv g extracellular domain was truncated and displayed as attachment proteins on m13 pha ... | 2017 | 28082163 |
| design and engineering of a transmissible antiviral defense. | we propose, model, and implement a novel system of population-level intervention against a virus. one context is a treatment against a chronic infection such as hiv. the underlying principle is a form of virus 'wars' in which a benign, transmissible agent is engineered to protect against infection by and spread of a lethal virus. in our specific case, the protective agent consists of two entities, a benign virus and a gene therapy vector mobilized by the benign virus. | 2016 | 27752283 |
| simulation of the m13 life cycle i: assembly of a genetically-structured deterministic chemical kinetic simulation. | to expand the quantitative, systems level understanding and foster the expansion of the biotechnological applications of the filamentous bacteriophage m13, we have unified the accumulated quantitative information on m13 biology into a genetically-structured, experimentally-based computational simulation of the entire phage life cycle. the deterministic chemical kinetic simulation explicitly includes the molecular details of dna replication, mrna transcription, protein translation and particle as ... | 2017 | 27644585 |
| hierarchical self-assembly of responsive organoplatinum(ii) metallacycle-tmv complexes with turn-on fluorescence. | here we report that the rod-like tobacco mosaic virus (tmv), having a negatively charged surface, can be assembled into three-dimensional micrometer-sized bundle-like superstructures via multiple electrostatic interactions with a positively charged molecular "glue", namely, a tetraphenylethylene (tpe)-based discrete organoplatinum(ii) metallacycle (tpe-pt-mc). due to the nanoconfinement effect in the resultant tmv/tpe-pt-mc complexes and the aggregation-induced emission (aie) activity of the tpe ... | 2016 | 27608138 |
| direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle. | the wall shear stress (wss) that a moving fluid exerts on a surface affects many processes including those relating to vascular function. wss plays an important role in normal physiology (e.g. angiogenesis) and affects the microvasculature's primary function of molecular transport. points of fluctuating wss show abnormalities in a number of diseases; however, there is no established technique for measuring wss directly in physiological systems. all current methods rely on estimates obtained from ... | 2015 | 27570611 |
| simulation of the m13 life cycle ii: investigation of the control mechanisms of m13 infection and establishment of the carrier state. | bacteriophage m13 is a true parasite of bacteria, able to co-opt the infected cell and control the production of progeny across many cellular generations. here, our genetically-structured simulation of m13 is applied to quantitatively dissect the interplay between the host cellular environment and the controlling interactions governing the phage life cycle during the initial establishment of infection and across multiple cell generations. multiple simulations suggest that phage-encoded feedback ... | 2017 | 27569186 |
| g5, a phage single-stranded dna-binding protein, fused with a nuclear localization signal, attenuates symptoms and reduces begomovirus-betasatellite accumulation in transgenic plants. | cotton leaf curl disease is caused by several monopartite begomoviruses and is the major threat to cotton production in the indian subcontinent. the disease has been shown to be associated with four distinct species, including cotton leaf curl kokhran virus (clcukov), and a specific betasatellite-cotton leaf curl multan betasatellite (clcumub). transgenic nicotiana benthamiana plants were produced which constitutively express the escherichia coli phage m13 encoded, sequence nonspecific single-st ... | 2016 | 27364491 |
| injected phage-displayed-vp28 vaccine reduces shrimp litopenaeus vannamei mortality by white spot syndrome virus infection. | white spot syndrome virus (wssv) is the most important viral pathogen for the global shrimp industry causing mass mortalities with huge economic losses. recombinant phages are capable of expressing foreign peptides on viral coat surface and act as antigenic peptide carriers bearing a phage-displayed vaccine. in this study, the full-length vp28 protein of wssv, widely known as potential vaccine against infection in shrimp, was successfully cloned and expressed on m13 filamentous phage. the functi ... | 2016 | 27241285 |
| virus based full colour pixels using a microheater. | mimicking natural structures has been received considerable attentions, and there have been a few practical advances. tremendous efforts based on a self-assembly technique have been contributed to the development of the novel photonic structures which are mimicking nature's inventions. we emulate the photonic structures from an origin of colour generation of mammalian skins and avian skin/feathers using m13 phage. the structures can be generated a full range of rgb colours that can be sensitivel ... | 2015 | 26334322 |
| cell-adhesive rgd peptide-displaying m13 bacteriophage/plga nanofiber matrices for growth of fibroblasts. | m13 bacteriophages can be readily fabricated as nanofibers due to non-toxic bacterial virus with a nanofiber-like shape. in the present study, we prepared hybrid nanofiber matrices composed of poly(lactic-co-glycolic acid, plga) and m13 bacteriophages which were genetically modified to display the rgd peptide on their surface (rgd-m13 phage). | 2014 | 26331065 |
| using the m13 phage as a biotemplate to create mesoporous structures decorated with gold and platinum nanoparticles. | by taking advantage of the physical and chemical properties of the m13 bacteriophage, we have used this virus to synthesize mesoporous silica structures. major coat protein p8 was chemically modified by attaching thiol groups. as we show, the resulting thiolated phage can be used as a biotemplate able to direct the formation of mesoporous silica materials. simultaneously, this thiol functionality acts as an anchor for binding metal ions, such as au(3+) and pt(4+), forming reactive m13-metal ioni ... | 2015 | 26275033 |
| virus-templated fret platform for the rational design of ratiometric fluorescent nanosensors. | we report here the construction of a bacteriophage m13-templated supramolecular nanosystem, i.e. m13-β-cd/ada-fitc/ada-rhb, which can be used as effective ratiometric fluorescent sensors for intracellular sensing. | 2015 | 26012560 |
| m13 bacteriophage and adeno-associated virus hybrid for novel tissue engineering material with gene delivery functions. | 2016 | 26010471 | |
| the nmr-rosetta capsid model of m13 bacteriophage reveals a quadrupled hydrophobic packing epitope. | filamentous phage are elongated semiflexible ssdna viruses that infect bacteria. the m13 phage, belonging to the family inoviridae, has a length of ∼1 μm and a diameter of ∼7 nm. here we present a structural model for the capsid of intact m13 bacteriophage using rosetta model building guided by structure restraints obtained from magic-angle spinning solid-state nmr experimental data. the c5 subunit symmetry observed in fiber diffraction studies was enforced during model building. the structure c ... | 2015 | 25587134 |
| detection of viruses by counting single fluorescent genetically biotinylated reporter immunophage using a lateral flow assay. | we demonstrated a lateral flow immunoassay (lfa) for detection of viruses using fluorescently labeled m13 bacteriophage as reporters and single-reporter counting as the readout. avitag-biotinylated m13 phage were functionalized with antibodies using avidin-biotin conjugation and fluorescently labeled with alexafluor 555. individual phage bound to target viruses (here ms2 as a model) captured on an lfa membrane strip were imaged using epi-fluorescence microscopy. using automated image processing, ... | 2015 | 25581289 |
| accelerated detection of viral particles by combining ac electric field effects and micro-raman spectroscopy. | a detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-raman spectroscopy is demonstrated for the case of m13 virus in water. the proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an ac electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. the same ... | 2015 | 25580902 |
| development and evaluation of single domain antibodies for vaccinia and the l1 antigen. | there is ongoing interest to develop high affinity, thermal stable recognition elements to replace conventional antibodies in biothreat detection assays. as part of this effort, single domain antibodies that target vaccinia virus were developed. two llamas were immunized with killed viral particles followed by boosts with the recombinant membrane protein, l1, to stimulate the immune response for envelope and membrane proteins of the virus. the variable domains of the induced heavy chain antibodi ... | 2014 | 25211488 |
| single virus detection by means of atomic force microscopy in combination with advanced image analysis. | in the present contribution virions of five different virus species, namely varicella-zoster virus, porcine teschovirus, tobacco mosaic virus, coliphage m13 and enterobacteria phage psp3, are investigated using atomic force microscopy (afm). from the resulting height images quantitative features like maximal height, area and volume of the viruses could be extracted and compared to reference values. subsequently, these features were accompanied by image moments, which quantify the morphology of t ... | 2014 | 25196422 |
| dying for good: virus-bacterium biofilm co-evolution enhances environmental fitness. | commonly used in biotechnology applications, filamentous m13 phage are non-lytic viruses that infect e. coli and other bacteria, with the potential to promote horizontal gene transfer in natural populations with synthetic biology implications for engineering community systems. using the e. coli strain tg1, we have investigated how a selective pressure involving elevated levels of toxic chromate, mimicking that found in some superfund sites, alters population dynamics following infection with eit ... | 2012 | 25114551 |
| using an aqueous two-phase polymer-salt system to rapidly concentrate viruses for improving the detection limit of the lateral-flow immunoassay. | the development of point-of-need (pon) diagnostics for viruses has the potential to prevent pandemics and protects against biological warfare threats. here we discuss the approach of using aqueous two-phase systems (atpss) to concentrate biomolecules prior to the lateral-flow immunoassay (lfa) for improved viral detection. in this paper, we developed a rapid pon detection assay as an extension to our previous proof-of-concept studies which used a micellar atps. we present our investigation of a ... | 2014 | 24942535 |
| virus-templated au and au/pt core/shell nanowires and their electrocatalytic activitives for fuel cell applications. | a facile synthetic route was developed to make au nanowires (nws) from surfactant-mediated bio-mineralization of a genetically engineered m13 phage with specific au binding peptides. from the selective interaction between au binding m13 phage and au ions in aqueous solution, au nws with uniform diameter were synthesized at room temperature with yields greater than 98 % without the need for size selection. the diameters of au nws were controlled from 10 nm to 50 nm. the au nws were found to be ac ... | 2012 | 24910712 |
| identification of peptides that bind hepatitis c virus envelope protein e2 and inhibit viral cellular entry from a phage-display peptide library. | hepatitis c virus (hcv) envelope protein e2 is required for the entry of hcv into cells. viral envelope proteins interact with cell receptors in a multistep process, which may be a promising target for the development of novel antiviral agents. in this study, a heptapeptide m13 phage-display library was screened for peptides that bind specifically to prokaryotically expressed, purified truncated hcv envelope protein e2. elisa assay was used to quantify the binding of the peptides to hcv e2 prote ... | 2014 | 24573486 |
| no effect of femtosecond laser pulses on m13, e. coli, dna, or protein. | data showing what appears to be nonthermal inactivation of m13 bacteriophage (m13), tobacco mosaic virus, escherichia coli (e. coli), and jurkatt t-cells following exposure to 80-fs pulses of laser radiation have been published. interest in the mechanism led to attempts to reproduce the results for m13 and e. coli. bacteriophage plaque-forming and bacteria colony-forming assays showed no inactivation of the microorganisms; therefore, model systems were used to see what, if any, damage might be o ... | 2014 | 24474502 |
| biomimetic virus-based colourimetric sensors. | many materials in nature change colours in response to stimuli, making them attractive for use as sensor platform. however, both natural materials and their synthetic analogues lack selectivity towards specific chemicals, and introducing such selectivity remains a challenge. here we report the self-assembly of genetically engineered viruses (m13 phage) into target-specific, colourimetric biosensors. the sensors are composed of phage-bundle nanostructures and exhibit viewing-angle independent col ... | 2014 | 24448217 |
| an analysis of tobacco mosaic virus replicative structures synthesized in vitro. | the rna structures synthesized in vitro by a crude enzyme complex from tobacco mosaic virus (tmv)-infected leaves have been analyzed; the major viral-specific products were similar to tmv-replicative form (rf) and-replicative intermediate (ri) in electrophoretic behavior and ribonuclease sensitivity. synthesis of these rf-like and ri-like structures neither required nor responded to added viral rna, but did require all four ribonucleotide triphosphates. enriched radiolabeled rf-like and ri-like ... | 1986 | 24307422 |
| fabrication of a silica nanocable using hydroxyl-group core-engineered filamentous virus. | biological materials with surface-active proteins can be genetically modified to bind target materials. in particular, filamentous-shaped m13 bacteriophages (m13 phage) are attractive scaffolds for functional nanostructures due to their highly ordered protein-coat surface. this paper demonstrates a simple method for fabricating silica nanocables along a modified m13 phage. the m13 phage was genetically engineered to display the amino acid serine on the surface to provide hydroxyl groups for a so ... | 2013 | 24205629 |
| self-assembly of rodlike virus to superlattices. | rodlike tobacco mosaic virus (tmv) has been found to assemble into superlattices in aqueous solution using the polymer methylcellulose to induce depletion and free volume entropy-based attractive forces. both transmission electron microscopy and small-angle x-ray scattering show that the superlattices form in both semidilute and concentrated regimes of polymer, where the free volume entropy and the depletion interaction are the dominant driving force, respectively. the superlattices are nacl and ... | 2013 | 24044529 |
| facile method for large scale alignment of one dimensional nanoparticles and control over myoblast orientation and differentiation. | a facile and robust method to align one-dimensional (1d) nanoparticles (nps) in large scale has been developed. using flow assembly, representative rod-like nanoparticles, including tobacco mosaic virus (tmv), gold nanorods, and bacteriophage m13, have been aligned inside glass tubes by controlling flow rate and substrate surface properties. the properties of 1d nps, such as stiffness and aspect ratio, play a critical role in the alignment. furthermore, these hierarchically organized structures ... | 2013 | 24004197 |
| a simple and rapid method to isolate purer m13 phage by isoelectric precipitation. | m13 virus (phage) has been extensively used in phage display technology and nanomaterial templating. our research aimed to use m13 phage to template sulfur nanoparticles for making lithium ion batteries. traditional methods for harvesting m13 phage from escherichia coli employ polyethylene glycol (peg)-based precipitation, and the yield is usually measured by plaque counting. with this method, peg residue is present in the m13 phage pellet and is difficult to eliminate. to resolve this issue, a ... | 2013 | 23807666 |
| virus-based photo-responsive nanowires formed by linking site-directed mutagenesis and chemical reaction. | owing to the genetic flexibility and error-free bulk production, bio-nanostructures such as filamentous phage showed great potential in materials synthesis, however, their photo-responsive behaviour is neither explored nor unveiled. here we show m13 phage genetically engineered with tyrosine residues precisely fused to the major coat protein is converted into a photo-responsive organic nanowire by a site-specific chemical reaction with an aromatic amine to form an azo dye structure on the surfac ... | 2013 | 23673356 |
| virus activated artificial ecm induces the osteoblastic differentiation of mesenchymal stem cells without osteogenic supplements. | biochemical and topographical features of an artificial extracellular matrix (aecm) can direct stem cell fate. however, it is difficult to vary only the biochemical cues without changing nanotopography to study their unique role. we took advantage of two unique features of m13 phage, a non-toxic nanofiber-like virus, to generate a virus-activated aecm with constant ordered ridge/groove nanotopography but displaying different fibronectin-derived peptides (rgd, its synergy site phsrn, and a combin ... | 2013 | 23393624 |
| generating thermal stable variants of protein domains through phage display. | often in protein design research, one desires to generate thermally stable variants of a protein or domain. one route to identifying mutations that yield domains that remain folded and active at a higher temperature is through the use of directed evolution. a library of protein domain variants can be generated by mutagenic pcr, expressed on the surface of bacteriophage m13, and subjected to heat, such that the unfolded forms of the domain, showing reduced or no binding activity, are lost during ... | 2012 | 23276752 |
| virus-poly(3,4-ethylenedioxythiophene) biocomposite films. | virus-poly(3,4-ethylenedioxythiophene) (virus-pedot) biocomposite films are prepared by electropolymerizing 3,4-ethylenedioxythiophene (edot) in aqueous electrolytes containing 12 mm liclo(4) and the bacteriophage m13. the concentration of virus in these solutions, [virus](soln), is varied from 3 to 15 nm. a quartz crystal microbalance is used to directly measure the total mass of the biocomposite film during its electrodeposition. in combination with a measurement of the electrodeposition charg ... | 2012 | 22856875 |
| engineered phage-based therapeutic materials inhibit chlamydia trachomatis intracellular infection. | developing materials that are effective against sexually transmitted pathogens such as chlamydia trachomatis (ct) and hiv-1 is challenging both in terms of material selection and improving bio-membrane and cellular permeability at desired mucosal sites. here, we engineered the prokaryotic bacterial virus (m13 phage) carrying two functional peptides, integrin binding peptide (rgd) and a segment of the polymorphic membrane protein d (pmpd) from ct, as a phage-based material that can ameliorate ct ... | 2012 | 22494890 |
| phage displayed hbv core antigen with immunogenic activity. | hepatitis b is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. the hepatitis b core antigen (hbcag) is one of the major viral proteins, which forms the inner core of hepatitis b virus (hbv) particles. in this study, filamentous bacteriophage m13 was genetically modified to display the polypeptides of hbcag in order to develop an alternative carrier system. hbcag gene was inserted into the minor coat protein (piii) ... | 2011 | 21915589 |
| RNA-binding domain in the nucleocapsid protein of gill-associated nidovirus of penaeid shrimp. | Gill-associated virus (GAV) infects Penaeus monodon shrimp and is the type species okavirus in the Roniviridae, the only invertebrate nidoviruses known currently. Electrophoretic mobility shift assays (EMSAs) using His(6)-tagged full-length and truncated proteins were employed to examine the nucleic acid binding properties of the GAV nucleocapsid (N) protein in vitro. The EMSAs showed full-length N protein to bind to all synthetic single-stranded (ss)RNAs tested independent of their sequence. Th ... | 2011 | 21857914 |
| [preparation and characterization of the recombinant protein containing immunomimetic peptide of benzo[a]pyrene]. | two recombinant plasmids were constructed. the first plasmid contained the hybrid gene composed of immunomimetic peptide of benzo[a]pyrene, of the protein piii of bacteriophage m13 and of cellulose binding domain encoding sequences. the second plasmid contained the hybrid gene composed of the signal peptide of the protein piii of bacteriophage m13, of immunomimetic peptide of benzo[a]pyrene, of the protein pill of bacteriophage m13 and of cellulose binding domain sequences. the obtained recombin ... | 2011 | 21789801 |
| virus-mediated fcc iron nanoparticle induced synthesis of uranium dioxide nanocrystals. | a reducing system involving m13 virus-mediated fcc fe nanoparticles was employed to achieve uranium reduction and synthesize uranium dioxide nanocrystals. here we show that metastable face-centered cubic (fcc) fe nanoparticles were fabricated around the surface of the m13 virus during the specific adsorption of the virus towards fe ions under a reduced environment. the fcc phase of these fe nanoparticles was confirmed by careful tem characterization. moreover, this virus-mediated fcc fe nanopart ... | 2008 | 21730559 |
| visualizing cell extracellular matrix (ecm) deposited by cells cultured on aligned bacteriophage m13 thin films. | topographical features ranging from micro- to nanometers can affect cell orientation and migratory pathways, which are important factors in tissue engineering and tumor migration. in our previous study, a convective assembly of bacteriophage m13 resulted in thin films which could be used to control the alignment of cells. however, several questions regarding its underlying reasons to dictate cell alignment remained unanswered. here, we further study the nanometer topographical features generated ... | 2011 | 21678980 |
| inhibition of bacterial conjugation by phage m13 and its protein g3p: quantitative analysis and model. | conjugation is the main mode of horizontal gene transfer that spreads antibiotic resistance among bacteria. strategies for inhibiting conjugation may be useful for preserving the effectiveness of antibiotics and preventing the emergence of bacterial strains with multiple resistances. filamentous bacteriophages were first observed to inhibit conjugation several decades ago. here we investigate the mechanism of inhibition and find that the primary effect on conjugation is occlusion of the conjugat ... | 2011 | 21637841 |
| variable epitope libraries: new vaccine immunogens capable of inducing broad human immunodeficiency virus type 1-neutralizing antibody response. | the extreme antigenic variability of human immunodeficiency virus (hiv) leads to immune escape of the virus, representing a major challenge in the design of effective vaccine. we have developed a novel concept for immunogen construction based on introduction of massive mutations within the epitopes targeting antigenically variable pathogens and diseases. previously, we showed that these immunogens carrying large combinatorial libraries of mutated epitope variants, termed as variable epitope libr ... | 2011 | 21600948 |
| anti-heparan sulfate peptides that block herpes simplex virus infection in vivo. | heparan sulfate (hs) and its highly modified form, 3-o sulfated heparan sulfate (3-os hs) contribute strongly to herpes simplex virus type-1 (hsv-1) infection in vitro. here we report results from a random m13-phage display library screening to isolate 12-mer peptides that bind specifically to hs, 3-os hs, and block hsv-1 entry. the screening identified representative candidates from two-different groups of anti-hs peptides with high positive charge densities. group 1 represented by g1 peptide ( ... | 2011 | 21596749 |
| virus-templated self-assembled single-walled carbon nanotubes for highly efficient electron collection in photovoltaic devices. | the performance of photovoltaic devices could be improved by using rationally designed nanocomposites with high electron mobility to efficiently collect photo-generated electrons. single-walled carbon nanotubes exhibit very high electron mobility, but the incorporation of such nanotubes into nanocomposites to create efficient photovoltaic devices is challenging. here, we report the synthesis of single-walled carbon nanotube-tio(2) nanocrystal core-shell nanocomposites using a genetically enginee ... | 2011 | 21516089 |
| [screening of polypeptides binding to porcine reproductive and respiratory syndrome virus by phage display library]. | to find specific polypeptides that bind porcine reproductive and respiratory syndrome virus (prrsv) with high affinity and inhibit replication of prrsv, we screened ligands on intact prrsv virion by phage display library. | 2011 | 21465799 |
| identification and safety evaluation of bacillus species occurring in high numbers during spontaneous fermentations to produce gergoush, a traditional sudanese bread snack. | gergoush is a naturally fermented sudanese bread snack produced in three fermentation steps (primary starter, adapted starter and final dough), followed by three baking steps for a half to one hour at above 200 °c. this study examines the microbiota of two sets of fermentations performed at a traditional production site in khartoum, sudan in 2006 and 2009, respectively. in 2006 four different milk/legume based primary starters (faba bean, chick pea, lentil and white bean) were sampled in order t ... | 2011 | 21429611 |
| genotypic characterization of cutaneous leishmaniasis at al baha and al qasim provinces (saudi arabia). | fifty samples of skin ulcers were collected from the western region of saudi arabia kingdom (al baha and al qasim) to study genotypic characterization of cutaneous leishmaniasis in this area. thirty-six samples were recorded as leishmania isolates. the same isolates were subsequently tested with fingerprinting with single arbitrary primers. the primers used derived from the core sequence of the phage m13, and the repeat sequences (gtg)5 and (gaca)4. the 36 isolates were all identified as leishma ... | 2011 | 21417928 |
| synthetic inter-species cooperation of host and virus for targeted genetic evolution. | cooperative inter-species interaction is another way of evolution in nature. such cooperation leading to mutual benefits provides a new view on the interaction of biological systems, and engineering such inter-species interaction offers an opportunity for diverse potential applications in biotechnology. here we show a synthetic system with artificially created inter-species cooperation of host and virus. a genetically engineered host (escherichia coli) provides nutrients and energies necessary f ... | 2011 | 21396406 |
| screening of specific binding peptide targeting blood vessel of human esophageal cancer in vivo in mice. | cancer of the esophagus and gastroesophageal junction remains a virulent malignancy with poor prognosis. rapid progresses were made in chemotherapeutic agents and the development of molecular markers allowed better identification of candidates for targeted therapy. this study aimed to identify the candidate peptides used for anti-angiogenic therapy of esophageal cancer by in vivo screening c7c peptide library for peptides binding specifically to blood vessels of human esophageal cancer. | 2011 | 21362285 |
| study of robustness of filamentous bacteriophages for industrial applications. | the development of a whole new class of industrial agents, such as biologically based nanomaterials and viral vectors, has raised many challenges for their large-scale manufacture, principally due to the lack of essential physical data and bioprocessing knowledge. a new example is the promise of filamentous bacteriophages and their derivatives. as a result, there is now an increasing need for the establishment of strong biochemical engineering foundations to serve as a guide for future manufactu ... | 2011 | 21328313 |
| characterisation of methacrylate monoliths for bacteriophage purification. | binding of three different bacteriophages (phages), namely t7, lambda and m13 on methacrylate monoliths was investigated. phage m13 exhibited the highest dynamic binding capacity of 4.5×10(13) pfu/ml while t7 and lambda showed capacity of 1×10(13) pfu/ml, all corresponding to values of around 1mg/ml. interestingly, capacity for lambda phage was increased 5-fold by increasing nacl concentration in a loaded sample from 0 to 0.2m while there was a constant capacity decrease for t7 and m13 phages. u ... | 2010 | 21238969 |
| selective cloning, characterization, and production of the culicoides nubeculosus salivary gland allergen repertoire associated with equine insect bite hypersensitivity. | salivary gland proteins of culicoides spp. have been suggested to be among the main allergens inducing ige-mediated insect bite hypersensitivity (ibh), an allergic dermatitis of the horse. the aim of our study was to identify, produce and characterize ige-binding salivary gland proteins of culicoides nubeculosus relevant for ibh by phage surface display technology. a cdna library constructed with mrna derived from c. nubeculosus salivary glands was displayed on the surface of filamentous phage m ... | 2010 | 21071100 |
| novel peptide inhibiting both tem-1 β-lactamase and penicillin-binding proteins. | 9g4h9, a catalytic antibody displaying β-lactamase-like activity, has been developed by the anti-idiotypic approach using β-lactamase as the first antigen. thus 9g4h9 represents the 'internal image' of β-lactamase. we selected a cyclic peptide anchored to a bacteriophage m13 library using 9g4h9 as the target. pep90 is a cyclic heptapeptide enclosed between two cysteine residues. we showed that pep90 could inhibit both tem-1 β-lactamase (k(i) = 333 μm) and several penicillin-binding proteins (ic ... | 2010 | 21044268 |
| phenotypic and genotypic characteristics of mycobacterium isolates from fighting fish betta spp. in malaysia. | mycobacteriosis due to mycobacteria is one of the most common bacterial diseases in ornamental fish. we describe here the phenotypic and genotypic characteristics of mycobacterium isolates from fighting fish betta spp. using atcc mycobacterium marinum, mycobacterium fortuitum and mycobacterium chelonae as references. a total of four isolates (m1, m2, m3, m4) were obtained from four out of 106 fish samples using selective agar, and identified to mycobacterium genus using acid-fast staining and 16 ... | 2010 | 20971487 |
| m13-microsatellite pcr and rdna sequence markers for identification of trichoderma (hypocreaceae) species in saudi arabian soil. | seven fungal isolates were identified as pan-global hypocrea/trichoderma species, from section trichoderma, on the basis of their morphology. these species were h. lixii/t. harzianum and h. orientalis/t. longibrachiatum. pcr-based markers with primer m13 (core sequence of phage m13) and internal-transcribed spacer sequences of ribosomal dna were used to confirm the identity of the two trichoderma species. sequence identification was performed using the trichokey version 2.0 barcode program and t ... | 2010 | 20957605 |
| enhancing the lateral-flow immunoassay for viral detection using an aqueous two-phase micellar system. | availability of a rapid, accurate, and reliable point-of-care (poc) device for detection of infectious agents and pandemic pathogens, such as swine-origin influenza a (h1n1) virus, is crucial for effective patient management and outbreak prevention. due to its ease of use, rapid processing, and minimal power and laboratory equipment requirements, the lateral-flow (immuno)assay (lfa) has gained much attention in recent years as a possible solution. however, since the sensitivity of lfa has been s ... | 2010 | 20865404 |
| binding of the dimeric deinococcus radiodurans single-stranded dna binding protein to single-stranded dna. | deinococcus radiodurans single-stranded (ss) dna binding protein (drssb) originates from a radiation-resistant bacterium and participates in dna recombination, replication, and repair. although it functions as a homodimer, it contains four dna binding domains (ob-folds) and thus is structurally similar to the escherichia coli ssb (ecossb) homotetramer. we examined the equilibrium binding of drssb to ssdna for comparison with that of ecossb. we find that the occluded site size of drssb on poly(dt ... | 2010 | 20795631 |
| phage display: concept, innovations, applications and future. | phage display is the technology that allows expression of exogenous (poly)peptides on the surface of phage particles. the concept is simple in principle: a library of phage particles expressing a wide diversity of peptides is used to select those that bind the desired target. the filamentous phage m13 is the most commonly used vector to create random peptide display libraries. several methods including recombinant techniques have been developed to increase the diversity of the library. on the ot ... | 2010 | 20659548 |
| [selecting the phage displaying tetrodotoxin mimic epitope by phage random peptide library]. | to screen the positive phage displaying the mimic epitope of tetrodotoxin (ttx) by using phage random peptide display library technology and to establish immunoassay for the detection of tetrodotoxin. | 2010 | 20568457 |
| purification of bacteriophage m13 by anion exchange chromatography. | m13 is a non-lytic filamentous bacteriophage (phage). it has been used widely in phage display technology for displaying foreign peptides, and also for studying macromolecule structures and interactions. traditionally, this phage has been purified by cesium chloride (cscl) density gradient ultracentrifugation which is highly laborious and time consuming. in the present study, a simple, rapid and efficient method for the purification of m13 based on anion exchange chromatography was established. ... | 2010 | 20538529 |
| biologically templated photocatalytic nanostructures for sustained light-driven water oxidation. | over several billion years, cyanobacteria and plants have evolved highly organized photosynthetic systems to shuttle both electronic and chemical species for the efficient oxidation of water. in a similar manner to reaction centres in natural photosystems, molecular and metal oxide catalysts have been used to photochemically oxidize water. however, the various approaches involving the molecular design of ligands, surface modification and immobilization still have limitations in terms of catalyti ... | 2010 | 20383127 |
| oligovalent fab display on m13 phage improved by directed evolution. | efficient display of antibody on filamentous phage m13 coat is crucial for successful biopanning selections. we applied a directed evolution strategy to improve the oligovalent display of a poorly behaving fab fragment fused to phage gene-3 for minor coat protein (g3p). the fab displaying clones were enriched from a randomly mutated fab gene library with polyclonal anti-mouse igg antibodies. contribution of each mutation to the improved phenotype of one selected mutant was studied. it was found ... | 2010 | 20012710 |
| molecular characterisation of sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in rio de janeiro, brazil. | an epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus sporothrix schenckii, is ongoing in rio de janeiro, brazil, in which cases of human infection are related to exposure to cats. in an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by dna-based typing methods 19 human and 25 animal s. schenckii isolates from the epidemic, as well as two control strains. to analyse the isolates, the random amplified polymorphic d ... | 2009 | 19820840 |
| replication of the single-stranded dna of the male-specific bacteriophage m13: circular forms of the replicative dna. | equilibrium centrifugation in caesium chloride, band sedimentation in alkaline solutions and electron microscopy have been used to study purified preparations of the m13 replicative dna. the buoyant density of the m13 replicative dna in a cscl density gradient is 1.701, compared to a density of 1-710 for escherichia coli dna. when the replicative dna is sedimented in alkaline solutions of cscl (p = 1.35), two components are observed with uncorrected s-values of 48.6 +/- 0.2 and 15.2 +/- 0.5. tre ... | 1966 | 19768865 |
| replication of the single-stranded dna of the male-specific bacteriophage m13. isolation of intracellular forms of phage-specific dna. | the intracellular dna has been isolated from escherichia coli f+ cells infected with the small male-specific bacteriophage m13 and fractionated on a methylated albumin-kieselguhr column. two infectious forms of phage-specific dna were isolated and identified as a double-stranded replicative form and single-stranded dna on the basis of their elution from the methylated albumin-kieselguhr column, infectivity to spheroplasts before and after heating, sedimentation at low ionic strength and buoyant ... | 1966 | 19768864 |
| sources of bacillus cereus contamination in a pasteurized zucchini purée processing line, differentiated by two pcr-based methods. | in previous work, raw materials used for processing pasteurized zucchini purée (zucchini, milk proteins and starch) and cultivation soil of zucchinis were found to be potential sources of stored product contamination with bacillus cereus. 134 b. cereus strains originating from these sources and from the stored product were typed using coliphage m13 sequence-based polymerase chain reaction (m13-pcr) and dna amplification fingerprinting. combined patterns from the two methods were compared using d ... | 2003 | 19719681 |
| asymmetric dipping of bacteriophage m13 coat protein with increasing lipid bilayer thickness. | knowledge about the vertical movement of a protein with respect to the lipid bilayer plane is important to understand protein functionality in the biological membrane. in this work, the vertical displacement of bacteriophage m13 major coat protein in a lipid bilayer is used as a model system to study the molecular details of its anchoring mechanism in a homologue series of lipids with the same polar head group but different hydrophobic chain length. the major coat proteins were reconstituted int ... | 2009 | 19715663 |
| viruses: incredible nanomachines. new advances with filamentous phages. | during recent decades, bacteriophages have been at the cutting edge of new developments in molecular biology, biophysics, and, more recently, bionanotechnology. in particular filamentous viruses, for example bacteriophage m13, have a virion architecture that enables precision building of ordered and defect-free two and three-dimensional structures on a nanometre scale. this could not have been possible without detailed knowledge of coat protein structure and dynamics during the virus reproductio ... | 2010 | 19680644 |
| transmembrane helix association affinity can be modulated by flanking and noninterfacial residues. | the gxxxg sequence motif mediates the association of transmembrane (tm) helices by providing a site of close contact between them. however, it is not sufficient for strong association. for example, both bacteriophage m13 major coat protein (mcp) and human erythrocyte protein glycophorin a (gpa) contain a gxxxg motif in their tm domains and form a homodimer, but the association affinity of mcp, measured by the toxcat in vivo assay, is dramatically weaker than that of gpa. even when all interfacia ... | 2009 | 19486666 |
| insertion of bacteriophage m13 coat protein into membranes. | 1982 | 19431483 | |
| surface rigidity change of escherichia coli after filamentous bacteriophage infection. | in this study, the feasibility using atomic force microscopy (afm) to study the interaction between bacteriophages (phages) and bacteria in situ was demonstrated here. filamentous phage m13 specifically infects the male escherichia coli, which expresses f-pili. after infection, e. coli become fragile and grows at a slower rate. afm provides a powerful tool for investigating these changes in a near-physiological environment. using high-resolution afm in phosphate-buffered saline, the damage to th ... | 2009 | 19366225 |
| from 'i' to 'l' and back again: the odyssey of membrane-bound m13 protein. | the major coat protein of the filamentous bacteriophage m13 is a surprising protein because it exists both as a membrane protein and as part of the m13 phage coat during its life cycle. early studies showed that the phage-bound structure of the coat protein was a continuous i-shaped alpha-helix. however, throughout the years various structural models, both i-shaped and l-shaped, have been proposed for the membrane-bound state of the coat protein. recently, site-directed labelling approaches have ... | 2009 | 19362002 |
| membrane protein frustration: protein incorporation into hydrophobic mismatched binary lipid mixtures. | bacteriophage m13 major coat protein was reconstituted in different nonmatching binary lipid mixtures composed of 14:1pc and 22:1pc lipid bilayers. challenged by this lose-lose situation of hydrophobic mismatch, the protein-lipid interactions are monitored by cd and site-directed spin-label electron spin resonance spectroscopy of spin-labeled site-specific single cysteine mutants located in the c-terminal protein domain embedded in the hydrophobic core of the membrane (i39c) and at the lipid-wat ... | 2009 | 19217858 |
| peptide-mediated reduction of silver ions on engineered biological scaffolds. | herein we report the spontaneous reduction of silver ions into nanostructures by yeast surface-displayed glutamic acid (e(6)) and aspartic acid (d(6)) peptides. light spectroscopy and electron microscopy reveal that silver ions are photoreduced in the presence of the polycarboxylic acid-containing peptides and ambient light, with an increase in reduction capability of e(6) expressing yeast over d(6) yeast. the importance of tethering peptides to a biological scaffold was inferred by observing th ... | 2008 | 19206318 |
| synthesis of a virus electrode for measurement of prostate specific membrane antigen. | though relatively unexploited in biosensor applications, phage display technology can provide versatile recognition scaffolds for detection of cancer markers and other analytes. this chapter details protocols for covalent attachment of viruses directly to electrodes for reagent-free detection of analytes in real-time. customization of binding specificity leverages selections with large phage display libraries prior to covalent attachment of the selected virus to the electrode. the methods descri ... | 2009 | 19159102 |
| primary endemic cryptococcosis gattii by molecular type vgii in the state of pará, brazil. | in order to study the infectious agents causing human disseminated cryptococcosis in the state of pará, north brazil, 56 isolates of cryptococcusspp. (54 isolated from cerebral spinal fluid and two from blood cultures) from 43 cases diagnosed between 2003-2007 were analysed. the species were determined through morphological and physiological tests and genotypes were determined by ura5-rflp and pcr-fingerprinting (wild-type phage m13). the following species and genotypes were identified: cryptoco ... | 2008 | 19148422 |
| controllable g5p-protein-directed aggregation of ssdna-gold nanoparticles. | we assembled single-stranded dna (ssdna) conjugated nanoparticles using the phage m13 gene 5 protein (g5p) as the molecular glue to bind two antiparallel noncomplementary ssdna strands. the entire process was controlled tightly by the concentration of the g5p protein and the presence of double-stranded dna. the g5p-ssdna aggregate was disintegrated by hybridization with complementary ssdna (c-ssdna) that triggers the dissociation of the complex. polyhistidine-tagged g5p was bound to nickel nitri ... | 2009 | 19072316 |
| malassezia furfur fingerprints as possible markers for human phylogeography. | malassezia furfur was the first species described within the cosmopolitan yeast genus malassezia, which now comprises 13 species. reported isolation rates of these species from healthy and diseased human skin show geographic variations. pcr-fingerprinting with the wild-type phage m13 primer (5'-gagggtggcggttct-3') was applied to investigate phylogeographic associations of m. furfur strains isolated from scandinavians residing permanently in greece, in comparison to clinical isolates from greek, ... | 2009 | 19020555 |
| oriented cell growth on self-assembled bacteriophage m13 thin films. | fibrillar m13 bacteriophages were used as basic building blocks to generate thin films with aligned nanogrooves, which, upon chemical grafting with rgd peptides, guide cell alignment and orient the cell outgrowth along defined directions. | 2008 | 18956063 |
| regional pattern of the molecular types of cryptococcus neoformans and cryptococcus gattii in brazil. | the molecular types of 443 brazilian isolates of cryptococcus neoformans and cryptococcus gattii were analyzed to determine their geographic distribution within brazil and their underlying host conditions. the following data, imported from previous epidemiological studies as well as two culture collections, were analyzed for: place of isolation, source (clinical or environmental), host risk factors, species, serotype, mating type, and molecular type. molecular typing by pcr-fingerprinting using ... | 2008 | 18797758 |
| single-molecule dna sequencing technologies for future genomics research. | during the current genomics revolution, the genomes of a large number of living organisms have been fully sequenced. however, with the advent of new sequencing technologies, genomics research is now at the threshold of a second revolution. several second-generation sequencing platforms became available in 2007, but a further revolution in dna resequencing technologies is being witnessed in 2008, with the launch of the first single-molecule dna sequencer (helicos biosciences), which has already b ... | 2008 | 18722683 |
| lysogenisation of spiroplasma citri by a type 3 spiroplasmavirus. | a short-tailed polyhedral spiroplasmavirus, ai, with a plaque morphology typical of temperate phages and a linear double-stranded dna genome which can circularise due to the presence of cohesive ends, was able to lysogenise spiroplasma citri. lysogenic sporoplasmas spontaneously released ai virus at a low level, and were immune to superinfection by the released virus, but not to infection by two serologically related viruses. these properties were retained following repeated cultivation in virus ... | 1985 | 18640554 |
| replication of tobacco mosaic virus. viii. characterization of a third subgenomic tmv rna. | in an earlier study we concluded that tobacco mosaic virus (tmv) infections engender a third subgenomic rna in infected tissue (p. palukaitis, f. garcia-arenal, m. a. sulzinski, and m. zaitlin (1983), virology 131, 533-545). this rna of approximate mw of 1.1 x 10(6), termed i1-rna, was shown to be polyribosome-associated and thus was presumed to serve as a messenger rna in vivo. upon in vitro translation of i1-rna in a rabbit reticulocyte lysate system, a major product of mw approximately 50k wa ... | 1985 | 18640547 |
| on the origin of the helper component of tobacco vein mottling virus: translational initiation near the 5' terminus of the viral rna and termination by uag codons. | the nature of the polypeptide products encoded by the 5'-terminal region of the rna of the potyvirus, tobacco vein mottling virus (tvmv), was investigated. single-stranded dna probes complementary to either nucleotides 1100-2100 or 2100-2820 from the 5' terminus of the rna were prepared by subcloning recombinant plasmids in bacteriophage m13. these were hybridized to tvmv rna, the dna:rna hybrids translated in a reticulocyte lysate cell-free translation system (hybrid-arrested translation), and ... | 1985 | 18639848 |
| subgenomic rnas with nucleotide sequences derived from rnas 1 and 2 of cucumber mosaic virus can act as messenger rnas in vitro. | encapsidated rnas of cucumber mosaic virus (cmv) were analyzed by hybridization to specific probes after gel electrophoresis. [32p]-complementary dna (cdna) probes were prepared by transcription of genomic rna 1 and rna 2 nucleotide sequences that had been cloned in a bacteriophage m13 vector. probes that correspond to unique sequences near the 3' ends of rna 1 and rna 2 revealed over 20 smaller rnas. the subgenomic rnas derived from each genomic rna were analyzed more definitively by hybrid sel ... | 1985 | 18639845 |
| direct electrical transduction of antibody binding to a covalent virus layer using electrochemical impedance. | electrochemical impedance spectroscopy is used to detect the binding of a 148.2 kda antibody to a "covalent virus layer" (cvl) immobilized on a gold electrode. the cvl consisted of m13 phage particles covalently anchored to a 3 mm diameter gold disk electrode. the ability of the cvl to distinguish this antibody ("p-ab") from a second, nonbinding antibody ("n-ab") was evaluated as a function of the frequency and phase of the measured current relative to the applied voltage. the binding of p-ab to ... | 2008 | 18590279 |
| recombinant adeno-associated viral vectors are deficient in provoking a dna damage response. | adeno-associated virus type 2 (aav2) provokes a dna damage response that mimics a stalled replication fork. we have previously shown that this response is dependent on ataxia telangiectasia-mutated and rad3-related kinase and involves recruitment of dna repair proteins into foci associated with aav2 dna. here, we investigated whether recombinant aav2 (raav2) vectors are able to produce a similar response. surprisingly, the results show that both single-stranded and double-stranded green fluoresc ... | 2008 | 18463154 |
| single-molecule dna sequencing of a viral genome. | the full promise of human genomics will be realized only when the genomes of thousands of individuals can be sequenced for comparative analysis. a reference sequence enables the use of short read length. we report an amplification-free method for determining the nucleotide sequence of more than 280,000 individual dna molecules simultaneously. a dna polymerase adds labeled nucleotides to surface-immobilized primer-template duplexes in stepwise fashion, and the asynchronous growth of individual dn ... | 2008 | 18388294 |
| real-time kinetics of restriction-modification gene expression after entry into a new host cell. | most type ii restriction-modification (r-m) systems produce separate restriction endonuclease (rease) and methyltransferase (mtase) proteins. after r-m system genes enter a new cell, protective mtase must appear before rease to avoid host chromosome cleavage. the basis for this apparent temporal regulation is not well understood. pvuii and some other r-m systems appear to achieve this delay by cotranscribing the rease gene with the gene for an autogenous transcription activator/repressor (the 'c ... | 2008 | 18334533 |
| opto-fluidic micro-ring resonator for sensitive label-free viral detection. | we have demonstrated sensitive label-free virus detection using the opto-fluidic ring resonator (ofrr) sensor. the ofrr is a novel sensing platform that integrates the microfluidics and photonic sensing technology with a low detection limit and small volume. in our experiment, filamentous bacteriophage m13 was used as a safe model system. virus samples were flowed through the ofrr whose surface was coated with m13-specific antibodies. we studied the sensor performance by monitoring in real-time ... | 2008 | 18299750 |
| immunoassays in nanoliter volume reactors using fluorescent particle diffusometry. | a model analyte, the m13 virus, was detected through the change in the brownian motion of a population of microparticles. epi-fluorescence microscopy was used to simultaneously track antibody-coated and bare microparticles to unambiguously measure the diffusion coefficient and demonstrate multiplexed detection. the sensitivity of the diffusometry assay was high enough that individual virus-to-particle binding ratios could be detected. analysis of the experimental errors indicated that the primar ... | 2008 | 18294011 |
| mapping protein-protein interactions with phage-displayed combinatorial peptide libraries. | this unit describes the process and analysis of affinity selecting bacteriophage m13 from libraries displaying combinatorial peptides fused to either a minor or major capsid protein. direct affinity selection uses target protein bound to a microtiter plate followed by purification of selected phage by elisa. alternatively, there is a bead-based affinity selection method. these methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus seq ... | 2003 | 18228422 |
| covalent virus layer for mass-based biosensing. | m13 virus particles were covalently attached to a planar gold-coated quartz crystal microbalance (qcm) through reaction with a self-assembled monolayer of n-hydroxysuccinimide thioctic ester, followed by incorporation of the blocking agent bovine serum albumin. this immobilization chemistry produced a phage multilayer having a coverage equivalent to approximately 6.5 close-packed monolayers of the virus. the properties of this "covalent virus surface" or cvs for the mass-based detection of a 148 ... | 2008 | 18198846 |
| transposon tn7 directs transposition into the genome of filamentous bacteriophage m13 using the element-encoded tnse protein. | the bacterial transposon tn7 has a pathway of transposition that preferentially targets conjugal plasmids. we propose that this same transposition pathway recognizes a structure or complex found during filamentous bacteriophage replication, likely by targeting negative-strand synthesis. the ability to insert into both plasmid and bacteriophage dnas that are capable of cell-to-cell transfer would help explain the wide distribution of tn7 relatives. | 2007 | 17921297 |
| presentation of the functional receptor-binding domain of the bacterial adhesin f17a-g on bacteriophage m13. | bovine enterotoxigenic escherichia coli (etec) carrying f17a fimbriae attach to the intestinal epithelium by means of the f17a-g adhesin. since filamentous bacteriophages can be employed for the display of foreign peptides, we tested the applicability of this system to f17a-g. the receptor-binding domain of the f17a-g adhesin was expressed on bacteriophage m13, as an amino-terminal fusion with the phage protein piii. this domain retained its n-acetyl-beta-d: -glucosamine binding activity. the ph ... | 2008 | 17701282 |
| influence of charge and flexibility on smectic phase formation in filamentous virus suspensions. | we present experimental measurements of the cholesteric-smectic phase transition of suspensions of charged semiflexible rods as a function of rod flexibility and surface charge. the rod particles consist of the bacteriophage m13 and closely related mutants, which are structurally identical to m13, but vary either in contour length and therefore ratio of persistence length to contour length, or surface charge. surface charge is altered in two ways; by changing solution ph and by comparing m13 wit ... | 2007 | 17677469 |
| inactivation of viruses by coherent excitations with a low power visible femtosecond laser. | resonant microwave absorption has been proposed in the literature to excite the vibrational states of microorganisms in an attempt to destroy them. but it is extremely difficult to transfer microwave excitation energy to the vibrational energy of microorganisms due to severe absorption of water in this spectral range. we demonstrate for the first time that, by using a visible femtosecond laser, it is effective to inactivate viruses such as bacteriophage m13 through impulsive stimulated raman sca ... | 2007 | 17550590 |
| probing the low-frequency vibrational modes of viruses with raman scattering--bacteriophage m13 in water. | raman spectroscopy is used to study low-wave-number (</=20 cm(-1)) acoustic vibrations of the m13 phage. a well-defined raman line is observed at around 8.5 cm(-1). the experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate raman selection rules derived from a bond polarizability model. the observed raman mode is shown to belong to one of the raman-active axial modes of the m13 phage protein coat. it is expected that the detection and ... | 2007 | 17477724 |
| the position of the gly-xxx-gly motif in transmembrane segments modulates dimer affinity. | although the intrinsic low solubility of membrane proteins presents challenges to their high-resolution structure determination, insight into the amino acid sequence features and forces that stabilize their folds has been provided through study of sequence-dependent helix-helix interactions between single transmembrane (tm) helices. while the stability of helix-helix partnerships mediated by the gly-xxx-gly (gg4) motif is known to be generally modulated by distal interfacial residues, it has not ... | 2006 | 17215886 |
| unit-length, single-stranded circular dnas of both polarity of begomoviruses are generated in escherichia coli harboring phage m13-cloned begomovirus genome with single copy of replication origin. | replication of genomic dnas of plant-pathogenic begomoviruses has been demonstrated in prokaryotes, which supported the possibility of analyzing dna replication process of begomoviruses in bacteria. however, previous studies indicated that the replication of begomovirus dnas in prokaryotes requires tandem constructs of viral genomes with at least two copies of the origin of replication (ori). in this study, phage m13 vector harboring the unit-length genome with only a single copy of ori of a mon ... | 2007 | 17204346 |
| fret study of membrane proteins: determination of the tilt and orientation of the n-terminal domain of m13 major coat protein. | a formalism for membrane protein structure determination was developed. this method is based on steady-state fret data and information about the position of the fluorescence maxima on site-directed fluorescent labeled proteins in combination with global data analysis utilizing simulation-based fitting. the methodology was applied to determine the structural properties of the n-terminal domain of the major coat protein from bacteriophage m13 reconstituted into unilamellar dopc/dopg (4:1 mol/mol) ... | 2007 | 17114224 |