Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| sequence-specific 1h-nmr assignment and secondary structure of the tyr41----his mutant of the single-stranded dna binding protein, gene v protein, encoded by the filamentous bacteriophage m13. | sequence-specific 1h-nmr assignments are reported for the tyr41----his (y41h) mutant of the single-stranded dna binding protein, encoded by gene v of the filamentous bacteriophage m13 (gvp). the mutant protein was chosen for this purpose because it exhibits significantly improved solubility characteristics over wild-type gvp [folkers et al. (1991) eur. j. biochem. 200, 139-148]. the secondary structure elements present in the protein are deduced from a qualitative interpretation of the nuclear o ... | 1991 | 1761038 |
| experience in shotgun sequencing a 134 kilobase pair dna molecule. | until now, large dna sequences have been obtained by cloning fragments of the target molecule into plasmid, cosmid or bacteriophage lambda vectors. the 134 kbp dna sequence of channel catfish virus was determined with relative ease by shotgun cloning of random fragments of genomic dna directly into a bacteriophage m13 vector, sequencing by dideoxynucleotide chain termination, and compilation of the data using staden's database handling programs. experience gained during this endeavour indicates ... | 1991 | 1768862 |
| shuttle plasmid vectors for lactobacillus casei and escherichia coli with a minus origin. | recombinant plasmids which can be used as shuttle vectors between escherichia coli and the industrially used strains of lactobacillus casei were constructed. they have replication regions closely related to those of pub110 and are likely to replicate by a rolling-circle mechanism via a plus-strand-specific dna intermediate in l. casei. both orientations of pala from the staphylococcal plasmid pc194 and those of the intergenic region from coliphage m13 are identified as active minus origins in l. ... | 1991 | 1781687 |
| [use of dna polymorphism detected by m13 phage dna in population studies]. | hypervariable "minisatellite" regions detected in human genome by wild-type phage m13 dna were found to have high polymorphism and somatic stability. analysis of individual specific patterns of hybridization of 44 human dnas from the kirov province is presented. molecular weight of fragments varied from 2 to 6 kb. mean frequency of a fragment in the population under study is p = 0.294 +/- 0.158. the mean number of fragments per individual is 11.6 +/- 1.8. comparison between the kirov population ... | 1991 | 1830281 |
| dna fingerprinting of the human intestinal parasite giardia intestinalis with hypervariable minisatellite sequences. | individual isolates of the giardia duodenalis group of protozoan intestinal parasites were identified by dna fingerprinting with hypervariable minisatellite sequences. a morphologically identical parasite is found in some forty different animal species. although the species name intestinalis is reserved for the human isolates, electrophoretic karyotyping suggests that most duodenalis isolates fall into the same species grouping. distinction based upon morphology, restriction endonuclease cleavag ... | 1991 | 1831167 |
| [analysis of genetic distances between populations using human dna "fingerprints" detected by a phage m13 dna probe]. | the frequencies of different electrophoretic bands in dna fingerprints detected by phage m13 dna probe in two populations of the kirov district were determined. the dna polymorphisms observed in these two populations were compared with each other and with those of the krasnodar populations, and pseudogenetic distances were calculated. the mean genetic distance between two kirov populations was 0.072, this between every kirov and krasnodar population being 0.21 and 0.22. | 1991 | 1838094 |
| plasmid co1ib contains an ssi signal close to the replication origin. | taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid psm32, a mini-co1ib plasmid. this ssi signal was situated in the 350-nt haeiii segment of the 1.8-kb s7 fragment, and located nearly 400 nt downstream of the origin of dna replication. introduction of the ssi signal into a mutant of filamentous phage m13 lacking oric resulted in restoration of phage growth and rfi dna synthesis. interestingly, dna homology studies showed that the nu ... | 1991 | 1857752 |
| dynamic light scattering from weakly bending rods: estimation of the dynamic bending rigidity of the m13 virus. | a theory is presented for the dynamic structure factor [s(k,t]) of weakly bending rods. this treatment is based on a discrete bead model for the brownian dynamics in which all bead motions associated with bending are constrained to occur in a plane perpendicular to the end-to-end vector, thus prohibiting extension or contraction along that axis. preset hydrodynamic interactions are incorporated in a numerically exact manner. the predicted normalized dynamic structure factor s(k,t)/s(k,0) should ... | 1991 | 1868169 |
| the replication termination signal terb of the escherichia coli chromosome is a deletion hot spot. | hybrids composed of phage m13, plasmid pbr322 and the termination signal of escherichia coli chromosome replication terb were used to show that arrest of dna synthesis creates a very efficient deletion hot spot. up to 80% of deletions occurring in these hybrids had one deletion end-point at terb provided that (i) terb was oriented to arrest m13 and pbr322 leading strand synthesis; and (ii) the host cells contained the tus protein necessary for arresting dna synthesis at terb. the position of ter ... | 1991 | 1868840 |
| characterization of wild-type and mutant m13 gene v proteins by means of 1h-nmr. | recording of good quality nmr spectra of the single-stranded dna binding protein gene v of the bacteriophage m13 is hindered by a specific protein aggregation effect. conditions are described for which nmr spectra of the protein can best be recorded. the aromatic part of the spectrum has been reinvestigated by means of two-dimensional total correlation spectroscopy. sequence-specific assignments were obtained for all of the aromatic amino acid residues with the help of a series of single-site mu ... | 1991 | 1879419 |
| assembly of combinatorial antibody libraries on phage surfaces: the gene iii site. | a phagemid system was developed for the monovalent display of combinatorial antibody fab libraries on the surface of filamentous phage m13. fab fragments were fused to the carboxyl-terminal domain of the gene iii protein. phage displaying fab fragments on their surface, or phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. the method may replace current antibody cloning techniques. | 1991 | 1896445 |
| regulation of expression of the genome of bacteriophage m13. gene v protein regulated translation of the mrnas encoded by genes i, iii, v and x. | with the aid of a binary plasmid in vivo testsystem it was demonstrated that the single-stranded dna binding protein encoded by gene v of bacteriophage m13 not only regulates the synthesis of its cognate dna replication proteins at the level of translation, but also of the assembly proteins and the coat proteins encoded by genes i and ii, respectively. furthermore, gene v protein functions as a translational autoregulator of its own synthesis. comparison of the mrna levels of genes i and x in th ... | 1991 | 1905158 |
| differentiation of species and strains among filamentous fungi by dna fingerprinting. | we have analyzed 11 strains and clones, representing five species (penicillium janthinellum, p. citrioviridae, p. chrysogenum, aspergillus niger, trichoderma harzianum) and three genera of filamentous fungi, for the presence of hypervariable loci in their genomes by hybridization with simple repeat oligonucleotides and the dna of phage m13. the oligonucleotide probes (ct)8, (gtg)5 and (gaca)4, as well as m13 dna, are informative probes for fingerprinting in all genera and species tested. the pro ... | 1991 | 1907892 |
| 2'-deoxy-6-thioguanosine 5'-triphosphate as a substrate for purified human dna polymerases and calf thymus terminal deoxynucleotidyltransferase in vitro. | 2'-deoxy-6-thioguanosine 5'-triphosphate (s6dgtp), a metabolite of the antileukemia agent 6-thioguanine, was evaluated as a substrate for purified human dna polymerases. using bacteriophage m13 single-strand dna as a template, s6dgtp substituted efficiently for dgtp and stimulated dna synthesis in reactions without dgtp, with dna polymerases alpha, delta, and gamma from the human leukemia cell line k562. the apparent km values for dgtp and s6dgtp were very similar, i.e., 1.2 microm each for poly ... | 1991 | 1921985 |
| dna fingerprints of sheep using an m13 probe. | the bacteriophage m13 dna was used to detect hypervariable minisatellites in several families of booroola sheep as well as merino and suffolk sheep. digestion of sheep dna gave rise to three to eight fragments with different restriction enzymes demonstrating considerable polymorphism between the different breeds. the length of informative dna fragments varied in size from 6 to 20kb. the dna fingerprints generated were individual specific and allowed for differentiation between closely related an ... | 1991 | 1928833 |
| the in situ aggregational and conformational state of the major coat protein of bacteriophage m13 in phospholipid bilayers mimicking the inner membrane of host escherichia coli. | the major coat protein of bacteriophage m13 has been reconstituted into phospholipids with a composition comparable to that found in the host (escherichia coli) inner membrane. reconstitution experiments have revealed conditions in which the alpha-oligomeric state is favored over the beta-polymeric state. discrimination between the two states of the membrane-bound coat protein (alpha-oligomeric and beta-polymeric states) has been achieved using high-performance size-exclusion chromatography and ... | 1991 | 1932035 |
| the escherichia coli terb sequence affects maintenance of a plasmid with the m13 phage replication origin. | replication initiated at the bacteriophage m13 origin can be affected by interaction of a properly oriented termination signal terb and the tus protein. the effect can be alleviated by overproduction of the m13 replication gene protein ii. | 1991 | 1938965 |
| formation of single and double strand breaks in dna ultraviolet irradiated at high intensity. | the induction of single-strand breaks (ssb) by two quantum processes in dna is well established. we now report that biphotonic processes result in double-strand breaks (dsb) as well. puc19 and bacteriophage m13 rf dna were irradiated using an excimer laser (248 nm) at intensities of 10(7), 10(9), 10(10) and 10(11) w/m2 and doses up to 30 kj/m2. the proportion of dna as supercoil, open circular, linear and short fragments was determined by gel electrophoresis. linear molecules were noted at fluen ... | 1991 | 1946694 |
| viable transmembrane region mutants of bacteriophage m13 coat protein prepared by site-directed mutagenesis. | bacteriophage m13 coat protein - a 50-residue protein located at the e. coli host membrane during phage reproduction - is subjected to cytoplasmic, membrane-bound, and dna-interactive environments during the phage life cycle. in research to examine the specific features of primary/secondary structure in the effective transmembrane (tm) region of the protein (residues 21-39: yigyawamvvvivgatigi) which modulate its capacity to respond conformationally to the progressive influences of these varying ... | 1991 | 1953741 |
| bst dna polymerase permits rapid sequence analysis from nanogram amounts of template. | a simple, rapid method is presented for the enzymatic sequence analysis of nanogram amounts of single-stranded or double-stranded dna. this approach employs the thermostable dna polymerase from bacillus sterothermophilus and exploits its ability to efficiently extend all of the template-primer complex, even at low substrate concentrations. the procedure requires few pipetting steps, no preannealing step and very short reaction time. this method can significantly reduce the cost associated with d ... | 1991 | 1954022 |
| isolation of the dna minisatellite probe mz 1.3 and its application to dna 'fingerprinting' analysis. | a minisatellite probe, mz 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. a repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. the mz 1.3 repeat shows variable homology of 53-73% to the repetitive sequence of the protein iii gene of the bacteriophage m13 genome. polymorphic restriction fragment patterns were found with mz 1.3 using the enzymes hinf i, bstn i, hae iii, mbo i, psti/pvu ii, and rsa i. an ave ... | 1990 | 1969380 |
| an improved method for the detection of dcm methylation in dna molecules. | the intrinsic insensitivity of ecorii recognition sites in rf dnas of phage m13 and vector m13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. since dcm- dna but not dcm(+)-methylated dna becomes susceptible under these conditions, this procedure constitutes an improvement of the dcm methylation assay. | 1990 | 1979301 |
| effects of mutations in glycosylation sites and disulphide bonds on processing, cd4-binding and fusion activity of human immunodeficiency virus envelope glycoproteins. | site-directed mutagenesis was used to study the biological significance of a disulphide bridge and two n-linked oligosaccharides in the cd4-binding region of the envelope glycoproteins of human immunodeficiency virus type 1. mutagenesis was performed in a phage m13 system at sites corresponding to the cysteine residue (amino acid 402) and the asparagine residues (390 and 447) of the env gene. the mutated env gene was inserted into a recombinant vaccinia virus under the control of the vaccinia vi ... | 1991 | 2045792 |
| genetic requirements for frameshift reversion induced by bulky dna adducts in m13 dna. | in order to analyze the genetic requirements and mechanisms of frameshift mutagenesis by activated aflatoxin b1 (afb1), in vitro-modified phage m13 replicative form (rf) dna was transfected into appropriate escherichia coli cells and +1 or -1 frameshift revertants in the lacz(alpha) gene were isolated. this analysis shows that both +1 and -1 frameshift mutagenesis by afb1 is significantly reduced in a umuc- background. on the other hand, in the absence of reca, +1 frameshift mutagenesis is parti ... | 1991 | 2067532 |
| molecular cloning of human satellite dna sequences and their use in detecting dna polymorphism. | a approximately 400 bp haeiii human genomic satellite dna band was cloned into puc18 to construct a partial library. a fragment of bacteriophage m13 containing a sequence homologous to the human minisatellite core was cloned in puc18 and was used as a probe to isolate a approximately 350 bp human satellite clone (ptrf5.6) from the partial library. other clones from this library showed a wide variation in terms of size and hybridization to the ptrf5.6 clone. human dna from different individuals w ... | 1990 | 2079331 |
| neutron and gamma-irradiation of bacteriophage m13 dna: use of standard neutron irradiation facility (snif). | we describe here the use of the van de graaff accelerator as a source of high energy neutrons for biological irradiation. single-stranded bacteriophage m13 dna was chosen as the system to determine the relative biological effectiveness of monoenergetic neutrons. a standard neutron irradiation facility (snif) was established using a 3 mv van de graaff accelerator. the 2d (d,n)3he nuclear reaction was used to produce neutron fluxes of 3 x 10(8) cm 2 sec-1 yielding dose rates as high as 50 gy h-1. ... | 1990 | 2098554 |
| a critical arginine in the large subunit of ribulose bisphosphate carboxylase/oxygenase identified by site-directed mutagenesis. | rapid inactivation by phenylglyoxal of ribulose bisphosphate carboxylase/oxygenase (ribulose-p2 carboxylase) from the cyanobacterium anacystis nidulans suggests the presence of an essential arginine, the modification of which is reduced in the presence of the substrate ribulose bisphosphate. arginine 292 in the large subunit of ribulose-p2 carboxylase from a. nidulans was chosen for site-directed mutagenesis studies on the basis of the complete conservation of this residue in corresponding seque ... | 1990 | 2108139 |
| translational regulation of m13 gene ii protein by its cognate single-stranded dna binding protein. | to unravel the mechanism by which the single-stranded dna binding protein encoded by gene v of the filamentous phage m13 regulates the synthesis of its cognate dna replication protein encoded by gene ii, an in vivo test system has been developed. the system consists of two recombinant plasmids with compatible replication origins. one plasmid contains m13 gene v under the control of the inducible arab promoter of salmonella typhimurium. the other plasmid contains a fusion gene, whose expression i ... | 1990 | 2110060 |
| stable expression of meningococcal class 1 protein in an antigenically reactive form in outer membranes of escherichia coli. | the entire gene encoding the class 1 outer membrane protein of neisseria meningitidis is located on a 2.2kb fragment, obtained on digestion of chromosomal dna with xbal. this xbal fragment from strain mc50 (subtype p1-16), which had previously been cloned in bacteriophage m13, has been transferred to the plasmid vector pmtl20. the resulting plasmid (ppora100) was propagated in escherichia coli (jm109) and cell lysates were subjected to sds-page. western blotting with anti-class 1 protein antibod ... | 1990 | 2117694 |
| the function of a leader peptide in translocating charged amino acyl residues across a membrane. | insertion of bacteriophage coat proteins into the membrane of infected bacterial cells can be studied as a model system of protein translocation across membranes. the coat protein of the filamentous bacteriophage pf3--which infects pseudomonas aeruginosa--is 44 amino acids in length and has the same basic structure as the coat protein of bacteriophage m13, which infects escherichia coli. however, unlike the pf3 coat protein, the m13 coat protein is synthesized as a precursor (procoat) with a typ ... | 1990 | 2124001 |
| random peptide libraries: a source of specific protein binding molecules. | libraries of random peptide sequences were constructed and screened to identify peptides that specifically bind to proteins. in one of these about 2 x 10(7) different 15-residue peptide sequences were expressed on the surface of the coliphage m13. each phage encoded a single random sequence and expressed it as a fusion complex with piii, a minor coat protein present at five molecules per phage. phage encoding nine different streptavidin-binding peptide sequences were isolated from this library. ... | 1990 | 2143033 |
| single-stranded-dna-dependent atpase from hela cells that stimulates dna polymerase alpha-primase activity: purification and characterization of the atpase. | a single-stranded dna-dependent atpase that cofractionates during the early stages of purification of a multiprotein dna polymerase alpha complex from hela cells has been purified to homogeneity. the atpase is part of a 16s multienzyme dna polymerase alpha complex that is fully active in sv40 dna replication in vitro. the atpase hydrolyzes atp to adp in a reaction that is completely dependent on the presence of dna. dna in single-stranded form is strongly preferred as a cofactor, and polydeoxynu ... | 1990 | 2148684 |
| detergent-solubilized m13 coat protein exists as an asymmetric dimer. observation of individual monomers by 15n, 13c and 1h nuclear magnetic resonance spectroscopy. | m13 coat protein is a simple integral membrane protein isolated from the filamentous coliphage m13. isotopic labels (13c and 15n) may be incorporated biosynthetically into the protein backbone. 13c nuclear magnetic resonance spectroscopy of carbonyl carbon atoms and two-dimensional 1h-detected 15n-1h heteronuclear shift correlation of coat protein in dodecylsulphate micelles have shown many residues throughout the protein to give rise to two distinct resonances of equal intensity. chemical shift ... | 1990 | 2157019 |
| esr of spin-labeled bacteriophage m13 coat protein in mixed phospholipid bilayers. | bacteriophage m13 major coat protein was spin-labeled with a nitroxide derivative of iodoacetamide, preferentially at the single methionine that is located in the hydrophobic region of the protein. the spin-labeled protein was incorporated at different lipid-to-protein ratios in phospholipid bilayers composed of dimyristoylphosphatidylglycerol (dmpg), dimyristoylphosphatidylcholine (dmpc), or the 1:1 molar mixture of these lipids. both conventional and saturation transfer (st) esr studies were p ... | 1990 | 2159806 |
| escherichia coli recq protein is a dna helicase. | the escherichia coli recq gene, a member of the recf recombination gene family, was set in an overexpression plasmid, and its product was purified to near-homogeneity. the purified recq protein exhibited a dna-dependent atpase and a helicase activity. without dna, no atpase activity was detected. the capacity as atpase cofactor varied with the type of dna in the following order: circular single strand greater than linear single strand much greater than circular or linear duplex. as a helicase, r ... | 1990 | 2164680 |
| the production and purification of pcr-derived recombinant simian immunodeficiency virus p27 gag protein; its use in detecting serological and t-cell responses in macaques. | the polymerase chain reaction (pcr) was used to amplify a region of the gag gene, encompassing the core protein p27, from genomic dna of cells infected with sivmac251 (32h isolate). the 767 base pair pcr product was cloned into the bacteriophage m13 and fully sequenced before sub-cloning into the expression vector puc19. the 30 kilodalton (kda) fusion protein of lacz-p27 was expressed as a soluble protein in e. coli jm101 cells and purified to greater than 90% purity by affinity chromatography. ... | 1990 | 2166751 |
| [the use of a molecular probe containing a specific cdna consisting of bacteriophage m13 for detecting the hepatitis a virus in clinical specimens]. | a probe was constructed containing a fragment of dna replica of hepatitis a virus (hav) rna within bacteriophage m13 single-stranded dna which allowed 10(-12) g of viral rna to be tested. hybridization of 32p-labeled probe with total rna from 559 samples of blood, saliva, and urine from patients with viral hepatitis a revealed the presence of hav rna in 14% of the samples. in the 1st week of the jaundice period hav rna was detected in 40% (15 positive samples out of the 39 tested), in the 2nd we ... | 1990 | 2176421 |
| [dnases of the cell nuclei: mn2+-dependent endonuclease]. | comparison of catalytic properties of a mn2(+)-dependent and a ca2+, mg2+ dependent endonucleases of rat liver cell nuclei was carried out. the mn2(+)-dependent endonuclease has mr 31 kda by sds-paag-electrophoresis; ph optimum 5.5; calcium-magnesium synergism less than 3 in rat liver dna, rf m13 dna and phage m13 dna. the rate of hydrolysis of single strand and double strand circular dna was the same. the mn2(+)-dependent endonuclease split dna by double hit manner, and didn't change the manner ... | 1990 | 2177666 |
| structure and dynamics of detergent-solubilized m13 coat protein (an integral membrane protein) determined by 13c and 15n nuclear magnetic resonance spectroscopy. | the major coat protein of the filamentous bacteriophage m13 is inserted as an integral protein in the inner membrane of the escherichia coli host upon infection. m13 coat protein is an ideal model membrane protein and has been the target of many biophysical studies. an overview is presented here of the application of nuclear magnetic resonance spectroscopy to the study of the structure and dynamics of m13 coat protein in several lipid-mimetic environments. the coat protein may be biosyntheticall ... | 1990 | 2190619 |
| [increase in stability of the recombinant phage m13 carrying escherichia coli genes rpijl by reducing expression of cloned genes]. | a recombinant phage mp9mw/rpob containing the bglii-b fragment of the escherichia coli rpljl-rpobc gene cluster was constructed on the basis of filamentous phage m13. stability of the phage was increased by insertion of a transcription terminator t beta' which blocked transcription of cloned genes from the plac of the vector. | 1990 | 2191898 |
| efficient translocation of positively charged residues of m13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion. | the coat protein of bacteriophage m13 is inserted into the escherichia coli plasma membrane as a precursor protein, termed procoat, with a typical leader peptide of 23 amino acid residues. its membrane insertion requires the electrochemical potential but not the cellular components seca and secy. since the electrochemical gradients result in the periplasmic side of the membrane being positively charged, the membrane potential could contribute to the transfer of the negatively charged central reg ... | 1990 | 2196172 |
| initial steps in protein membrane insertion. bacteriophage m13 procoat protein binds to the membrane surface by electrostatic interaction. | bacteriophage m13 procoat protein is synthesized on free polysomes prior to its assembly into the inner membrane of escherichia coli. as an initial step of the membrane insertion pathway, the precursor protein interacts with the cytoplasmic face of the inner membrane. we have used oligonucleotide-directed mutagenesis to study the regions of the procoat protein involved in membrane binding. we find that there is an absolute requirement for positively charged amino acids at both ends of the protei ... | 1990 | 2202592 |
| interactions between potential anti-tumour 2,5-bis(1-aziridinyl)-1,4-benzoquinone derivatives and glutathione: reductive activation, conjugation and dna damage. | the interaction between glutathione and potential anti-tumour 3,6-disubstituted 2,5-bis(1-aziridinyl)-1,4-benzoquinone (babq) derivatives has been studied using u.v. spectrophotometry and h.p.l.c. the formation of babq-glutathione adducts was demonstrated in vitro for the babq parent compound (tw13), triaziquone (2,3,5-tris(1-aziridinyl)-1,4-benzoquinone) and for babq derivatives containing halogen substituents. the clinically-used babq derivative diaziquone (azq; 2,5-bis(1-aziridinyl)-3,6-bis(e ... | 1990 | 2205226 |
| hydrogen exchange kinetics in a membrane protein determined by 15n nmr spectroscopy: use of the inept experiment to follow individual amides in detergent-solubilized m13 coat protein. | the coat protein of the filamentous coliphage m13 is a 50-residue polypeptide which spans the inner membrane of the escherichia coli host upon infection. amide hydrogen exchange kinetics have been used to probe the structure and dynamics of m13 coat protein which has been solubilized in sodium dodecyl sulfate (sds) micelles. in a previous 1h nuclear magnetic resonance (nmr) study [o'neil, j. d. j., & sykes, b. d. (1988) biochemistry 27, 2753-2762], multiple exponential analysis of the unresolved ... | 1990 | 2207075 |
| deletion mutagenesis in m13 by polymerase chain reaction using universal sequencing primers. | a simple procedure is described for the efficient deletion of large dna sequences. the method involves a combination of oligonucleotide-directed mutagenesis in bacteriophage m13 and amplification of the mutagenized product by polymerase chain reaction. in contrast to other protocols employing polymerase chain reaction, synthesis of only one specific primer is required. the efficiency of heteroduplex formation between mutagenic primers directing large deletions and single-stranded template is dis ... | 1990 | 2221375 |
| export and purification of a cytoplasmic dimeric protein by fusion to the maltose-binding protein of escherichia coli. | a hybrid between the maltose-binding protein (male) of escherichia coli and the gene 5 protein (g5p) of phage m13 was constructed at the genetic level. male is a monomeric and periplasmic protein while g5p is dimeric and cytoplasmic. the hybrid (male-g5p) was synthesized in large amounts from a multicopy plasmid and efficiently exported into the periplasmic space of e. coli. the export was dependent on the integrity of the signal peptide. male-g5p was purified from a periplasmic extract by affin ... | 1990 | 2226455 |
| complex between single-stranded dna and gene 5 protein of bacteriophage m13 studied with linear dichroism and ultraviolet absorption. | we have studied complexes between the gene 5 protein (gp5) of bacteriophage m13 and various polynucleotides, including single-stranded dna, using ultraviolet absorption and linear dichroism. upon complex formation the absorption spectra of both the protein and the polynucleotides change. the protein absorption changes indicate that for at least two of the five tyrosine residues per protein monomer the environment becomes less polar upon binding to the polynucleotides but also to the oligonucleot ... | 1990 | 2258937 |
| detection of genomic alterations in carcinogen-induced mouse liver tumors by dna fingerprint analysis. | dna fingerprint analysis was used to study structural abnormalities in the genome of mouse liver tumor cells. liver tumors were induced in three strains of mice, namely c57bl/6j, c3h/he and b6c3f1, by a single injection of 20 micrograms/g body wt. diethylnitrosamine on day 15 after birth. dna from liver tumors was digested with hinfl restriction enzyme and hybridized on southern blots with wild-type bacteriophage m13 dna as probe. the resulting fingerprints of tumor dna were compared with those ... | 1990 | 2278631 |
| specificity of spontaneous mutation in the laci gene cloned into bacteriophage m13. | we have studied the specificity of spontaneous mutation in the laci gene of escherichia coli cloned into bacteriophage m13. the comparison of the spectrum of 85 spontaneous mutations with that of the laci gene carried on an e. coli f' episone revealed the following characteristics: (i) base substitution was predominant, accounting for 80% of spontaneous events compared with only 11% on the f' episome; (ii) among the base substitutions, the majority were g:c----a:t transitions (86%); (iii) not on ... | 1990 | 2300081 |
| structure of the dna binding wing of the gene-v encoded single- stranded dna binding protein of the filamentous bacteriophage m13. | the structure in solution of a beta-loop in mutant y41h of the single-stranded dna binding protein encoded by gene-v of the filamentous phage m13 has been elucidated using 2-dimensional 1h-nuclear magnetic resonance techniques. furthermore, these studies enabled us to demonstrate that an identical structural element is present in wild-type gene-v-protein and that this element intimately is involved in the binding of gene-v-protein to single-stranded dna. it is shown that the structure of the dna ... | 1990 | 2307226 |
| modification of dna by bile acids: a possible factor in the etiology of colon cancer. | bile acids have been implicated as promoters and cocarcinogens in the etiology of colon cancer and as comutagens and mutagens in bacteria. these observations suggest the hypothesis that bile acids may interact directly with dna. we treated the single stranded circular dna of phage m13 with bile acids and found that the transfection efficiency of this dna declined up to a 1000-fold. this result suggests that bile acids can damage dna and thus may play an important role in the etiology of colon ca ... | 1990 | 2317781 |
| assessment of inbreeding by dna fingerprinting: development of a calibration curve using defined strains of chickens. | by analyzing dna fingerprints of chickens from seven well-defined genetic groups, a calibration curve was established relating the degree of inbreeding with the average band frequency, allelic frequency and band sharing. the probe used was bacteriophage m13 dna and digestion of the genomic dna was carried out with the mspi restriction enzyme. the analysis also provided an estimate of the average allelic frequency at a hypervariable locus and the average mutation frequency per locus and generatio ... | 1990 | 2341028 |
| [nonisotope variant of genomic fingerprinting based on the phage m13 dna in kinship studies in man]. | dna "fingerprinting" with the 32p-labeled or biotinylated m13 phage dna was used in man parentage studies. it was shown that the non-isotopic method, having sensitivity, similar to that of isotopic one, gave higher level of band resolution. the method is safe and requires 3-5 h to visualize the bands. | 1990 | 2344951 |
| dna fingerprinting of the intestinal parasite giardia duodenalis with the m13 phage genome. | a dna fingerprint procedure has been established for the intestinal parasite, giardia duodenalis. this permits the identification of individual strains from both human and animal sources. analysis of strain movement, resurgence and variation is now possible. the fingerprint probe is based on the tandem repetitive sequence found in bacteriophage m13 which hybridizes to a set of hypervariable polymorphic minisatellite sequences found in higher eukaryotes. this probe recognizes a weakly homologous ... | 1990 | 2358315 |
| [the use of filamentous phage m13 in protein engineering]. | m13b1 vector based on the filamentous phage m13 has been constructed. m13b1 phage carries the gene of resistance to ampicillin and contains the unique site of recognition for bamhi restriction endonuclease in gene viii coding for the major coat protein. bamhi restriction site has been inserted into the gene of the major coat protein by means of oligonucleotide directed mutagenesis. the synthetic dna fragment coding for the model peptides has been inserted through bamhi site into the m13b1 dna. t ... | 1990 | 2362594 |
| analysis of time-resolved fluorescence anisotropy in lipid-protein systems. i. application to the lipid probe octadecyl rhodamine b in interaction with bacteriophage m13 coat protein incorporated in phospholipid bilayers. | fluorescent probes located in heterogeneous environments give rise to anomalous time-resolved fluorescence anisotropy. a simple analytical expression of anisotropy has been derived for the case of a small difference in local fluorescence lifetimes. the expression has the diagnostic advantage that the time dependence of the fluorescence anisotropy can be predicted from the differences in fluorescence lifetimes and residual anisotropies of the probes located in different sites. using this model, t ... | 1990 | 2369870 |
| analysis of time-resolved fluorescence anisotropy in lipid-protein systems. ii. application to tryptophan fluorescence of bacteriophage m13 coat protein incorporated in phospholipid bilayers. | the subnanosecond fluorescence and motional dynamics of the tryptophan residue in the bacteriophage m13 coat protein incorporated within pure dioleoylphosphatidylcholine (dopc) as well as dioleoylphosphatidylcholine/dioleoylphosphatidylglycerol (dopc/dopg) and dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (dmpc/dmpg) bilayers (80/20 w/w) with various l/p ratio have been investigated. the fluorescence decay is decomposed into four components with lifetimes of about 0.5, 2.0, 4.5 ... | 1990 | 2369871 |
| subtyping hepatitis b virus dna in free or integrated forms by amplification of the s-gene sequences by the polymerase chain reaction and single-track sequencing for adenine. | the s-gene fragments of hepatitis b virus (hbv) dna in serum, or integrated in chromosomes of human hepatoma cells (plc/prf/5), were amplified by the polymerase chain reaction, cloned into an m13 phage vector, and then sequenced only for adenine. the subtype determinant d or y was established by the presence or absence of adenine as nucleotide 365, and w or r by that of nucleotide 479 in the s gene. the results were identical with those obtained by enzyme immunoassay with monoclonal antibodies. ... | 1990 | 2370286 |
| biochemical and biophysical studies on the folding of the core region of the origin of replication of bacteriophage m13. | dna oligonucleotides with the sequence corresponding to the plus strand origin of replication of the filamentous bacteriophage m13 are studied. biochemical structure probing and uv melting studies, supplemented with initial nmr experiments, are used to investigate structural features of a 51-nucleotides long synthetic oligonucleotide and two oligonucleotides that are integral parts of this latter molecule. the results demonstrate the feasibility and complementarity of the use of methidiumpropyl. ... | 1990 | 2395637 |
| sequence analysis of the translational elongation factor 3 from saccharomyces cerevisiae. | the gene yef-3 encoding the elongation factor for protein synthesis in saccharomyces cerevisiae is an essential gene as shown by one-step gene disruption and is located on chromosome xii as determined by orthogonal field alternation gel electrophoresis. the nucleotide sequence of the gene was determined from a sequential series of subclones generated from the yef-3 gene cloned into bacteriophage m13. the homol1 sequence and the rpg box, which are considered to be enhancer elements involved in co ... | 1990 | 2404974 |
| initiation of dna replication on single-stranded dna templates catalyzed by purified replication proteins of bacteriophage lambda and escherichia coli. | initiation of bacteriophage lambda dna replication at the chromosomal origin depends on the lambda o and p replication proteins. these two viral initiators, together with an escherichia coli protein fraction, promote the replication in vitro of single-stranded circular dna chromosomes such as that of bacteriophage m13. this nonspecific strand initiation reaction, which we have termed the "lambda single-strand replication reaction," has now been established with eight purified proteins, each of w ... | 1985 | 2408273 |
| multiple crosslinks of proteins s7 and s9 to domains 3 and 4 of 16s ribosomal rna in the escherichia coli 30s particle. | rna-protein cross-links were introduced into escherichia coli 30s subunits by treatment with 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. 16s rrna, cross-linked to 30s ribosomal proteins, was isolated and hybridized with seven single-stranded bacteriophage m13-dna probes. these probes, each carrying an inserted rdna fragment, were used to select contiguous rna sections covering domains 3 and 4 (starting at nucleotide 868 and ending at the 3'oh terminus) of the 16s rrna. the proteins covalently ... | 1986 | 2429836 |
| biosynthesis of reovirus-specified polypeptides. molecular cdna cloning and nucleotide sequence of the reovirus serotype 1 lang strain bicistronic s1 mrna which encodes the minor capsid polypeptide sigma 1a and the nonstructural polypeptide sigma 1bns. | human reovirus serotype 1 lang strain s1 mrna, which encodes the minor capsid cell attachment protein sigma 1a and the nonstructural protein sigma 1bns, was cloned as a cdna:mrna heteroduplex in escherichia coli using phage m13. the lang strain s1 mrna is 1462 nucleotides in length and possesses two open reading frames. the first begins at nt 14 and has a coding capacity of 418 amino acids, sufficient to account for sigma 1a; the second begins at nt 75 and has a coding capacity of 119 amino acid ... | 1986 | 2430568 |
| a single base change in the shine-dalgarno region of 16s rrna of escherichia coli affects translation of many proteins. | a single base mutation was constructed at position 1538 of escherichia coli 16s rrna, changing a cytidine to a uridine. this position is in the shine-dalgarno region, thought to be involved in base-pairing to mrna during initiation of protein synthesis. the mutation was constructed by using a synthetic oligodeoxynucleotide that differs in sequence by one base from the wild-type sequence of 16s rrna. this oligonucleotide was used as a primer on single-stranded dna of phage m13, into which was clo ... | 1987 | 2440027 |
| a system for rapid dna sequencing with fluorescent chain-terminating dideoxynucleotides. | a dna sequencing system based on the use of a novel set of four chain-terminating dideoxynucleotides, each carrying a different chemically tuned succinylfluorescein dye distinguished by its fluorescent emission is described. avian myeloblastosis virus reverse transcriptase is used in a modified dideoxy dna sequencing protocol to produce a complete set of fluorescence-tagged fragments in one reaction mixture. these dna fragments are resolved by polyacrylamide gel electrophoresis in one sequencing ... | 1987 | 2443975 |
| isolation of cdnas of scrapie-modulated rnas by subtractive hybridization of a cdna library. | we have developed a subtractive cloning procedure based on the hybridization of single-stranded cdna libraries constructed in pi h3m, a vector containing the phage m13 origin of replication. we have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. dna sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein ii, and the b chain of alpha-crystallin; the latter two may represent a response to stress. | 1988 | 2456582 |
| rapid synthesis and cloning of complementary dna from any rna molecule into plasmid and phage m13 vectors. | we describe several modifications of the gubler and hoffman procedure [gene 25 (1983) 263-269] for complementary dna (cdna) synthesis that expand the versatility of this method for the rapid synthesis and cloning of double-stranded (ds) cdna. these modifications include: (1) the combination of first and second strand synthesis into a single two-step reaction, which reduces the time for synthesis of blunt-ended ds-cdna to less than 4 h. (2) the use of random hexadeoxyribonucleotide primers (rp) f ... | 1988 | 2464528 |
| nearest neighbor influences on dna polymerase insertion fidelity. | the kinetics of forming all possible single base substitution errors are measured for drosophila melanogaster dna polymerase alpha and avian myeloblastosis virus reverse transcriptase. seventeen sites along bacteriophage m13 dna are investigated so that effects of nearest neighbor base stacking on misinsertion kinetics can be evaluated. polymerase alpha appears to be more error prone than reverse transcriptase. polymerase alpha forms transversion mispairs at rates comparable to transition mispai ... | 1989 | 2474545 |
| inhibition of human immunodeficiency virus reverse transcriptase by 2',3'-dideoxynucleoside triphosphates: template dependence, and combination with phosphonoformate. | the 2',3'-dideoxynucleoside triphosphates (ddntps) are potent substrate analog inhibitors of human immunodeficiency virus (hiv) reverse transcriptase and have clinical utility in the treatment of acquired immunodeficiency syndrome. several issues regarding the interaction of these compounds with hiv reverse transcriptase were examined. the potency of unsubstituted ddntps and the 3'-azido analog of dttp (azttp) was influenced by the choice of template. both compounds were more potent with the com ... | 1989 | 2474897 |
| specificity of the binding of bacteriophage m13 encoded gene-5 protein to dna and rna studied by means of fluorescence titrations. | the fluorescence quenching of the bacteriophage m13 encoded gene-5 protein was used to study its binding characteristics to different polynucleotides. experiments were performed at different salt concentrations and in some instances at different temperatures. the affinity of the protein depends on the base and sugar composition of the polynucleotides involved and may differ appreciably, i.e. by orders of magnitude. the salt dependence of binding is within experimental accuracy equal for all sing ... | 1985 | 2482044 |
| interaction of nucleolar phosphoprotein b23 with nucleic acids. | the interaction of eukaryotic nucleolar phosphoprotein b23 with nucleic acids was examined by gel retardation and filter binding assays, by fluorescence techniques, and by circular dichroism. all studies utilized protein prepared under native conditions by a newly developed purification procedure. electrophoretic gel mobility shift assays with phage m13 dna suggested that protein b23 is a single-stranded nucleic acid binding protein. this was confirmed in competition binding assays with native o ... | 1989 | 2482073 |
| crosslinking of ribosomal proteins s4, s5, s7, s8, s11, s12 and s18 to domains 1 and 2 of 16s rrna in the escherichia coli 30s particle. | rna-protein crosslinks were introduced into escherichia coli 30s ribosomal subunits by treatment with 1-ethyl-3(3-dimethylaminopropyl)carbodiimide (edc). complexes of 16s rrna cross-linked to 30s ribosomal proteins were isolated and hybridized with a series of single-stranded bacteriophage m13-rdna probes. these probes, each carrying an inserted rdna fragment, were used to select contiguous 16s rrna sections covering all of domain 1 and the major part of domain 2 (starting at the 5'-p terminus a ... | 1989 | 2505863 |
| dna polymerase from sulfolobus acidocaldarius. replication at high temperature of long stretches of single-stranded dna. | the activity of a homogeneous dna polymerase from the thermophilic archaebacterium, sulfolobus acidocaldarius, on a singly primed, single-stranded recombinant phage m13 dna has been examined. at the optimal temperature (70 to 75 degrees c) this template is efficiently replicated in ten minutes using a ratio of enzyme molecule to primed-template of 0.8. analysis of dna products during the course of polymerization shows that species of quite homogeneous size are observed and that the number of pri ... | 1989 | 2511325 |
| [the use of dna from phage m13 for the analysis of interindividual polymorphism of human dna as demonstrated by a population study in krasnodar city]. | hypervariable "minisatellite" regions detected in human genome by wild-type m13 dna were found to have high polymorphism and somatic stability. analysis of individual specific patterns of 34 human dnas from krasnodar population is presented. the observed length of fragments ranged from 2 to 6 kb. the mean frequency of a fragment in the population under study is p = 0.247 +/- 0.171, the mean number of fragments per individual being x = 9.35 +/- 1.95. the mean probability of individual identificat ... | 1989 | 2533908 |
| genetic analysis of atttn7, the transposon tn7 attachment site in escherichia coli, using a novel m13-based transduction assay. | the large (14 kb; kb = 10(3) bases) bacterial transposon, tn7 (encoding resistance to trimethoprim and streptomycin/spectinomycin), has unusual properties. like other elements, tn7 transposes with low efficiency and low target-site specificity, but tn7 also transposes, with high frequency in a unique orientation, to a preferred "attachment" site, called atttn7, in the escherichia coli chromosome and similarly into plasmids containing atttn7. we developed a novel bacteriophage m13-based assay sys ... | 1989 | 2544739 |
| [selective inhibition of 3'-5'-exonuclease activity of dna-polymerase i from escherichia coli by a fluoride ion]. | the effect of naf on the enzymatic activities of the large fragment of e. coli dna polymerase i (klenow enzyme-ke) with different dna-substrates was studied. it was shown that fluoride ion at concentrations of 5-10 mm efficiently inhibits the 3'----5' exonuclease activity of ke but does not affect the polymerase activity of the enzyme. selective inhibition of the 3'----5' exonuclease activity of ke is mg-dependent and is observed with double- or single-stranded dnas. in reaction with the 14-mer ... | 1989 | 2544797 |
| localization of dna repair synthesis by human cell extracts to a short region at the site of a lesion. | double-stranded bacteriophage m13 dna molecules were constructed containing a single specifically placed 2-(acetylamino)fluorene adduct or a single 4'-hydroxymethyl-4,5',8-trimethylpsoralen monoadduct. these circular dna molecules were used to analyze in vitro dna repair synthesis by cell extracts from normal human lymphoid cell lines. both types of lesions stimulate dna repair synthesis at the site of the adduct. dna repair synthesis induced by the 2-(acetyl-amino)fluorene adduct took place in ... | 1989 | 2557339 |
| cystein 402 of hiv gp 120 is essential for cd4-binding and resistance of gp 120 to intracellular degradation. | a dna fragment encoding the cd4-binding region of human immunodeficiency virus type 1 (hiv) gp 120 was excised from an sv40-based expression vector containing gp 160, and subcloned into phage m13 for site-directed mutagenesis. mutant vectors were constructed and cv-1 cells were transfected with constructs, where cys402 was substituted for a serine, and metabolically labelled with [3h]-n-acetylglucosamine (glcn). radioimmunoprecipitation with an hyperimmunserum, specific for gp 120/gp 160, and su ... | 1989 | 2558637 |
| spin-label esr of bacteriophage m13 coat protein in mixed lipid bilayers. characterization of molecular selectivity of charged phospholipids for the bacteriophage m13 coat protein in lipid bilayers. | bacteriophage m13 major coat protein has been incorporated at different lipid/protein ratios in lipid bilayers consisting of various ratios of dimyristoylphosphatidylcholine (dmpc) to dimyristoylphosphatidylglycerol (dmpg). spin-label esr experiments were performed with phospholipids labeled at the c-14 position of the sn-2 chain. for m13 coat protein recombinants with dmpc alone, the relative association constants were determined for the phosphatidylcholine, phosphatidylglycerol, and phosphatid ... | 1989 | 2559776 |
| a phage-linked immunoabsorbant system for the detection of pathologically relevant antigens. | this report describes a novel system for the immunological detection of immobilized antigen. the detection of herpes simplex virus (hsv) antigen was used as an example. bacteriophage m13, containing the e. coli lac z gene, was used as the "reporter" molecule in an immunoassay which is otherwise analogous to the enzyme-linked immunoabsorbant assay (elisa). briefly, hsv infected cells were incubated with a mouse monoclonal antibody specific for hsv antigen, followed by rabbit anti-mouse serum and ... | 1989 | 2561061 |
| fimbriae of bacteroides nodosus: protein engineering of the structural subunit for the production of an exogenous peptide. | the pattern of sequence variation between bacteroides nodosus fimbrial subunits of different serotypes suggests a degree of flexibility, which might be exploited for protein engineering approaches for the expression of other peptides. we have tested this using the well-characterized peptide epitope from vp1 of foot-and-mouth disease virus (fmdv), residues 144-159: lrgdlqvlaqkvartl (strain 01-bfs). using bacterial codon usage, several oligonucleotides were designed for the substitution of this se ... | 1989 | 2564674 |
| hypervariable dna fingerprinting in escherichia coli: minisatellite probe from bacteriophage m13. | extensive restriction-fragment-length polymorphism was revealed in escherichia coli strains by using a region of the bacteriophage m13 genome as a dna hybridization probe. this variation was observed across natural strains, in clinical samples, and to a lesser extent in laboratory strains. the sequence in m13 which revealed this fingerprint pattern was a region of the gene iii coat protein, which contains two clusters of a 15-base-pair repeat. oligonucleotides made to a consensus of these repeat ... | 1989 | 2565332 |
| dna primase-dna polymerase alpha from simian cells: sequence specificity of initiation sites on simian virus 40 dna. | unique single-stranded regions of simian virus 40 dna, phage m13 virion dna, and several homopolymers were used as templates for the synthesis of (p)pprna-dna chains by cv-1 cell dna primase-dna polymerase alpha. intact rna primers, specifically labeled with an rna capping enzyme, were typically 6 to 8 ribonucleotides long, although their lengths ranged from 1 to 9 bases. the fraction of intact rna primers 1 to 4 ribonucleotides long was 14 to 73%, depending on the template used. rna primer leng ... | 1985 | 2582240 |
| non-radioactive hybridization probes prepared by the chemical labelling of dna and rna with a novel reagent, photobiotin. | a photo-activatable analogue of biotin, n-(4-azido-2-nitrophenyl)-n'-(n-d-biotinyl-3-aminopropyl)-n'-methyl-1,3- propanediamine (photobiotin), has been synthesized and used for the rapid and reliable preparation of large amounts of stable, non-radioactive, biotin-labelled dna and rna hybridization probes. upon brief irradiation with visible light, photobiotin formed stable linkages with single- and double-stranded nucleic acids yielding probes which were purified from excess reagent by 2-butanol ... | 1985 | 2582358 |
| [stable recombinants of bacteriophage m13 and plasmid pbr322]. | two recombinants between the phage m13 and the plasmid pbr322 were isolated, analyzing the plasmid content of over one hundred colonies obtained by transduction. the study of the structure of both recombinants indicates that a fragment of the m13 genome has been integrated to pbr322. in both cases, the fragment contains a part of the phage replication region inserted either in the vicinity or within the pbr322 replicon. the fact that the phage and plasmid replicons seem to be involved in the rec ... | 1989 | 2640765 |
| site-specific recombination at orit of plasmid r1162 in the absence of conjugative transfer. | r1162 is efficiently comobilized during conjugative transfer of the self-transmissible plasmid r751. bacteriophage m13 derivatives that contain two directly repeated copies of orit, the site on r1162 dna required in cis for mobilization, were constructed. phage dna molecules underwent recombination during infection of escherichia coli, with the product retaining a single functional copy of orit. recombination was strand specific and depended on r1162 gene products involved in mobilization, but d ... | 1989 | 2644236 |
| mutagenic specificity of alkylated and oxidized dna bases as determined by site-specific mutagenesis. | this work demonstrates the use of the tools of site-specific mutagenesis to study the mutagenic activity of two dna adducts, o6-methylguanine and cis-thymine glycol. the former adduct is one of the methylated bases formed by carcinogenic and mutagenic alkylating agents. it was built into the single-stranded genome of bacteriophage m13 and replicated in escherichia coli (e. coli). the mutation frequency of o6-methylguanine was 0.4% in physiologically normal cells. in cells in which the repair sys ... | 1989 | 2665597 |
| [cloning and expression of the hemagglutinin gene of influenza virus subtype h1 in escherichia coli]. | the full-length copy of the hemagglutinin gene of influenza virus was inserted into m13 phage dna. the dna sequence coding for the hydrophobic prepeptide was removed from the gene by oligonucleotide-directed mutagenesis. the possibilities of expression of the full-length and mutant genes in e. coli were investigated. the beta-galactosidase-hemagglutinin fusion proteins were isolated. the fusion proteins exhibited specific binding to antiviral antibodies. this binding could be competitively inhib ... | 1989 | 2671677 |
| environmental modulation of m13 coat protein tryptophan fluorescence dynamics. | the effects of detergent [deoxycholate (doc) and phospholipid [dimyristoylphosphatidylcholine (dmpc)] environments on the rotational dynamics of the single tryptophan residue 26 of bacteriophage m13 coat protein have been investigated by using time-resolved single photon counting measurements of the fluorescence intensity and anisotropy decay. the total fluorescence decay of tryptophan-26 is complex but rather similar in doc as compared to dmpc when analyzed in terms of a lifetime distribution ( ... | 1989 | 2675970 |
| aggregation-related conformational change of the membrane-associated coat protein of bacteriophage m13. | the state of the coat protein of bacteriophage m13, reconstituted into amphiphilic media, has been investigated. the in situ conformation of the coat protein has been determined by using circular dichroism. minimum numbers for the protein aggregation in the system have been determined after disruption of the lipid-protein system and subsequent uptake of the protein in cholate micelles. the aggregational state and conformation of the protein were affected by (1) the method of coat protein isolati ... | 1989 | 2690954 |
| [genomic "dactyloscopy" with the use of bacteriophage m13 as a dna probe (the expertise of material evidence and personal identification)]. | in this article the authors give a scientific evaluation of genetic dactyloscopy method in which the sites of human chromosomal dna, possessing structural polymorphism, act as genetic markers. technology of genome "dactyloscopy" including both the series of standard conventional methods and new methods is presented. the method is highly sensitive and requires small amounts of material for investigation. a practical case is described when genome "dactyloscopy" gave positive results which led to a ... | 1989 | 2694453 |
| detection and location of single-base mutations in large dna fragments by immunomicroscopy. | a technique whereby single-base mutations can be detected by immunomicroscopy of dna heteroduplexes is described. four constructs of the filamentous phage m13 were prepared so as to differ by a single base at the same site. heteroduplexes were prepared and reacted with a water-soluble carbodiimide, with polyclonal antibodies specific for the carbodiimide, and then with a second antibody linked to an electrondense marker. electron microscopy of the heteroduplexes indicated that the label was loca ... | 1989 | 2744763 |
| evidence for unlinked rrn operons in the planctomycete pirellula marina. | southern hybridization of rrnas to chromosomal bamhi-digested dna of the eubacterium pirellula marina revealed the presence of two sets of 16s and 23s rrna genes. the two copies of the 23s rrna genes, located on 11- and about 13-kilobase (kb) inserts, were isolated from a lambda bacteriophage charon 35 library. the 11-kb fragment was cloned directly into pbr322, while a 5.4-kb bamhi-psti rdna subfragment of the approximately 13-kb insert was cloned into puc18. both recombinant plasmids, ppi1100 ... | 1989 | 2768196 |
| rapid isolation of dna from complex biological samples using a novel capture reagent--methidium-spermine-sepharose. | we have synthesized and analyzed the functional properties of a novel dna capture reagent containing a methidium moiety attached to a sepharose bead by a spermine linker. dna present in a biological fluid or other complex sample binds to the reagent. the dna-capture reagent complex is then separated from the sample by centrifugation and the dna is released from the reagent by brief incubation in 0.1 to 0.5 n naoh or koh. capture of dna from complex samples is independent of the salt concentratio ... | 1989 | 2780316 |
| effect of bacteriophage m13 infection on phosphorylation of dnak protein and other escherichia coli proteins. | 1. the effects of infection with the filamentous phage m13 on the phosphorylation of escherichia coli proteins were studied. phosphorylated proteins were labeled with [32p]orthophosphate and analyzed by the o'farrell two-dimensional gel technique and autoradiography. 2. phage infection was shown to induce significant changes in the pattern of protein phosphorylation. at least eight different proteins were found to be phosphorylated to a larger extent while seven others were, by contrast, much le ... | 1987 | 2822422 |
| spin-label electron spin resonance study of bacteriophage m13 coat protein incorporation into mixed lipid bilayers. | the major coat protein of bacteriophage m13 was incorporated in mixed dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (80/20 w/w) vesicles probed with different spin-labeled phospholipids, labeled on the c-14 atom of the sn-2 chain. the specificity for a series of phospholipids was determined from a motionally restricted component seen in the electron spin resonance (esr) spectra of vesicles with the coat protein incorporated. at 30 degrees c and ph 8, the fraction of motionally r ... | 1987 | 2827755 |
| characterization of the promoter region of the bacillus subtilis spoiie operon. | mutations that define the spoiie locus of bacillus subtilis block sporulation at an early stage and recently were shown to prevent the proteolytic processing of sigma e (sigma 29) into its active form, an event that is believed to control critical changes in gene expression during the second hour of development. by taking advantage of two tn917-mediated insertional mutations in spoiie, we have cloned dna spanning the locus. gene disruption experiments with subcloned fragments transferred to inte ... | 1988 | 2832371 |
| promoter-detection vectors for escherichia coli with multiple useful features. | two promoter-detection vectors have been constructed which enable the cloning and characterization of promoters recognized by the rna polymerase of escherichia coli k-12. the intergenic region of phage m13 dna, present in opposite orientations in the two vectors, permits the preparation of single-stranded dna of either strand of the insert thus facilitating oligodeoxyribonucleotide heteroduplex mutagenesis and sequencing of both strands by the dideoxy method of chain termination. after mutagenes ... | 1988 | 2841194 |
| [subcloning of dna fragments of the simian adenovirus sa7 oncogene in bacteriophage m13]. | the xmai/psti and xmai dna fragments of adenovirus sa7 oncogene and the adjacent region (16.7% of the physical map of sa7 left end dna) were recloned in m13 bacteriophages mp8 and mp9 in order to obtain the singlestranded fragments eia and eib from the dna region of monkey adenovirus sa7 located on the recombinant plasmid pasp carrying the dna apsti fragment including the adenovirus sa7 oncogene. | 1988 | 2842670 |
| mutational spectrum and recombinogenic effects induced by aminofluorene adducts in bacteriophage m13. | double-stranded replicative form (rfi) dna of bacteriophage m13 strain m13mp10 which carries partial lacz gene has been modified in vitro to various extents with n-hydroxy-2-amino-fluorene (n-oh-af) and then transfected into e. coli cells. high-performance liquid chromatography (hplc) analysis results demonstrate that the sole adduct (95%) formed in modified dna is n-(deoxyguanosine-8-yl)-2-aminofluorene (dg-c8-af). approximately 20 adducts per rfi molecule constitute 1 lethal event when plaque- ... | 1988 | 2843766 |